通过固相微萃取-气相色谱-质谱联用(solid phase micro-extraction-gas chromatography-mass spectrometer, SPME-GC-MS)法测定了β-环糊精在溶液中对香芹酮的保留率及两者之间的形成常数,并用冷冻干燥法制备了β-环糊精与香芹酮的包合物,采用扫描电镜(scanning electron microscopy,SEM)、红外光谱(infrared spectrum,IR)、X-衍射(X-ray diffraction,XRD)及热重分析(thermogravimetric analysis,TGA)对包合物进行结构表征。结果表明,香芹酮与β-环糊精在水溶液中形成了1∶1的包合物,且两者之间的形成常数为1 066.7 mol-1。冷冻干燥法制备的包合物的包合率为75.04%,SEM以及XRD分析结果都表明,β-环糊精在包合前后,其晶体形态发生了变化;IR和TG结果证明β-环糊精与香芹酮形成了包合物。由此得出,β-环糊精适合包埋香芹酮,可以提高香芹酮的热稳定性,从而使香芹酮的应用更加广泛。
The retention rate of carvone in β-cyclodextrin solution and the formation constant between these two compounds were determined by SPME-GC-MS. The inclusion complex of β-cyclodextrin and carvone was prepared by freeze-drying method, and it was characterized by scanning electron microscopy (SEM), infrared spectroscopy (IR), X-ray diffraction (XRD) and thermogravimetric analysis (TGA). The results showed that carvone and β-cyclodextrin formed an inclusion complex in a ratio of 1∶1 in aqueous solution, and the formation constant was 1 066.7 mol-1. Moreover, the encapsulation efficiency of the complex was 75.04%, and SEM and XRD showed that the crystal morphology of β-cyclodextrin changed before and after inclusion. Furthermore, IR and TGA also showed that β-cyclodextrin and carvone formed an inclusion complex. Therefore, it can be concluded that β-cyclodextrin is suitable for embedding carvone to improve its thermal stability, which will be beneficial for broadening the application of carvone.
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