为了研究圆弧青霉发酵生成右旋糖酐酶的过程及动力学,测定发酵过程的菌体质量浓度、右旋糖酐酶酶活以及总糖(底物)质量浓度随时间的变化,分别采用Logistic方程、Luedeking-Piret方程和类Luedeking-Piret方程对实验数据进行非线性拟合,获得了圆弧青霉菌菌体生长、右旋糖酐酶生成和底物消耗的动力学模型,相关系数R2分别为0.994、0.992、0.991。对获得的动力学模型进行分析,计算值与实验值的误差合理,所建立的发酵动力学模型能较好地反映出圆弧青霉菌发酵产右旋糖酐酶的过程,为控制和预测发酵过程提供了理论基础。
In order to study the process and kinetics of dextranase production by Penicillium cyclopium, changes in cell density, dextranase activity and total sugar along fermentation time were measured. Logistic equation, Luedeking-piret equation and Luedeking-piret-like equation were used to arrange the experimental data to obtain kinetic models of cell growth (R2=0.994), dextranase synthesis (R2=0.992) and substrate consumption (R2=0.991), respectively. All established kinetic models had reasonable errors between calculated values and experimental values, therefore, they can reflect the fermentation process of Penicillum. cyclopium in a better way to produce dextranase, which provides a theoretical basis for controlling and predicting the fermentation process.
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