The study investigated the formation of Listeria monocytogenes biofilms at 32 ℃ and 10 ℃, as well as treatments on the biofilms with lavender essential oil and ‘8-4’ brand disinfectant at 0.5, 1, 2, 4 folds of MIC. Crystal violet staining, XTT assay and PMA-qPCR were used to evaluate their removal effects. The results showed that L. monocytogenes could form stable biofilms at both temperatures, and the biomass of biofilms formed at 32℃ was significantly higher than that at 10 ℃. After 3 h treatment with four concentrations of lavender essential oil, the removal rates of biofilms formed at 32 ℃ and 10 ℃ were 62%-82% and 25%-56%, respectively. Besides, the removal rates of four concentrations of ‘8-4’ brand disinfectant against warm and cold biofilms were 47%-78% and 36%-56%, respectively. Moreover, after 3 h treatment with 4 folds of MIC (6.4%, v/v) lavender essential oil, the residual viable cell counts in cold biofilms were more than twice compared to that of warm biofilms. This study revealed that cold biofilms of L. monocytogenes were more resistant, and lavender essential oil had more significant removal effect than that of ‘8-4’ brand disinfectant, which shows positive application of plant essential oil in controlling L. monocytogenes.
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