为了探究鼠尾草提取物对冷藏(4 ℃)鲢鱼肌原纤维蛋白(myofibrillar protein,MP)结构和功能特性的影响,以质量浓度1 g/L茶多酚(TP)、0.1 g/L二丁基羟基甲苯(BHT)处理作为参照,试验组用10、20、30 g/L的鼠尾草提取物处理,在冷藏期内考察MP结构和功能指标的变化。结果表明,随着冷藏时间延长,所有组鲢鱼MP的溶解度、巯基含量、乳化性质、凝胶硬度和弹性、保水性以及白度值逐渐下降,表面疏水性、羰基含量逐渐上升,且20 g/L鼠尾草提取物处理的鲢鱼MP羰基含量比空白对照组降低了31.09%,显著抑制了鲢鱼MP的氧化,同时在一定程度促进了凝胶的形成。 添加鼠尾草提取物、TP、BHT均能显著抑制鲢鱼 MP的氧化,且20 g/L的鼠尾草提取物抑制效果最好,表明鼠尾草提取物是一种应用前景广阔的抗氧化剂。
This study investigated the effects of Salvia officinalis extracts on the structural and functional properties of Hypophthalmichthys molitrix myofibrillar protein (MP) stored at 4 ℃. MP was treated with 10 g/L, 20 g/L, and 30 g/L Salvia officinalis extracts, and 1 g/L tea polyphenol and 0.1 g/L dibutylhydroxytoluene (BHT) were used as references. With the prolongation of refrigerating time, the solubility, sulfhydryl content, emulsifying activity index (EAI) and emulsion stability index (ESI), gel hardness and elasticity, water retention capacity as well as whiteness of the MP in all experimental groups gradually decreased, while surface hydrophobicity and carbonyl content gradually increased. The carbonyl content of MP treated with 20 g/L Salvia officinalis extracts was 31.09% lower than that of the control, indicating that it significantly inhibited the oxidation of MP and also promoted the formation of gel to some extent. Therefore, this study reveals that Salvia officinalis can be used as a promising antioxidant.
[1] 王帮国.白鲢鱼肌肉脂肪氧合酶构象和酶学特性及其脂肪氧化调控研究[D].合肥:合肥工业大学,2017.
[2] 张进杰,阙婷婷,曹玉敏,等.壳聚糖、Nisin涂膜在鲢鱼块冷藏保鲜中的应用[J].中国食品学报,2013,13(8):132-139.
[3] 杨华,张建斌,吴晓,等.冰温贮藏对鲢、草、鲤鱼糜制品品质的影响[J].食品科学,2016,37(12):273-278.
[4] 王涛.川西鼠尾草属植物观赏资源调查与引种及果实和叶片形态学研究[D].雅安:四川农业大学,2012.
[5] SANTOS-GOMES P C, SEABRA R M, ANDRADE P B, et al. Determination of phenolic antioxidant compounds produced by calli and cell suspensions of sage (Salvia officinalis L.)[J]. Journal of Plant Physiology, 2003, 160(9): 1 025-1 032.
[6] PIZZALE L, BORTOLOMEAZZI R, VICHI S, et al. Antioxidant activity of sage (Salvia officinalis and S. fruticosa) and oregano (Origanmn onites and O. indercedens) extracts related to their phenolic compound content[J]. Journal of the Science of Food and Agriculture, 2002, 82(14): 1 645-1 651.
[7] 章林,冷雪娇,黄明.鼠尾草提取物多酚含量与抗氧化活性的研究[J].食品工业科技,2012,33(19):143-146.
[8] 李玉邯,麻莹,陈宇飞,等.鼠尾草对猪肉脯感官品质和抗氧化性能的影响[J].中国调味品,2016,41(12):55-58.
[9] MCCARTHY T L, KERRY J P, DERRY J F, et al. Assessment of the antioxidant potential of natural food and plant extracts in fresh and previously frozen pork patties[J].Meat Science, 2001, 57(2):177-184.
[10] ZHANG Z Y,YANG Y L,TANG X Z,et al. Effects of ionic strength on chemical forces heat-induced myofibrillar protein gel[J]. Food Science and Technology Research,2015,21(4):597-605.
[11] CHIN K B, GO M Y, XIONG Y L. Konjac flour improved textural and water retention properties of transglutaminase-mediated, heat-induced porcine myofibrillar protein gel: Effect of salt level and transglutaminase incubation[J]. Meat Science, 2009, 81(3): 565-572.
[12] 刘琴.金枪鱼肌原纤维蛋白冻藏过程中生化及流变特性研究[D].上海:上海海洋大学,2013.
[13] BAHAR T, KORAY K. The effects of an iron-catalyzed oxidation system on lipids and proteins of dark muscle fish[J]. Food Chemistry,2007,104(2):754-760.
[14] LAEMMLI U K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4[J]. Nature,1970,227(5 259) :680-685.
[15] AGYARE K K, ADDO K, XIONG Y L. Emulsifying and foaming properties of transglutaminase-treated wheat gluten hydrolysate as influenced by pH, temperature and salt[J]. Food Hydrocolloids, 2009, 23(1): 72-81.
[16] AGYARE K K, XIONG Y L, ADDO K. Influence of salt and pH on the solubility and structural characteristics of transglutaminase-treated wheat gluten hydrolysate[J]. Food Chemistry, 2008, 107(3): 1 131-1 137.
[17] 李艳青. 蛋白质氧化对鲤鱼蛋白结构和功能性的影响及其控制技术[D]. 哈尔滨: 东北农业大学, 2013.
[18] SALVADOR P, TOLDRA M, SAGUER E, et al. Microstructure-function relationships of heat-induced gels of porcine haemoglobin[J]. Food Hydrocolloids, 2009, 23(7): 1 654-1 659.
[19] PARK J W. Functional protein additives in surimi gels[J]. Journal of Food Science, 1994, 59(3): 525-527.
[20] 牛思思,汪建明,贺雅欣,等.羟基自由基氧化对蛋清蛋白质结构的影响[J].食品工业科技,2017,38(8):113-117.
[21] 丁景,王晓君,白登荣,等.五种抗氧化剂处理对微冻贮藏泥鳅肌原纤维蛋白功能性质的影响[J].食品与发酵工业,2018,44(8):231-238.
[22] THANONKAEW A, BENJAKUL S, VISESSANGUAN W, et al. The effect of antioxidants on the quality changes of cuttlefish(Sepia pharaonis) muscle during frozen storage[J]. LWT-Food Science and Technology, 2008, 41(1):161-169.
[23] 荣建华,甘承露,丁玉琴,等.低温贮藏对脆肉鲩鱼肉肌动球蛋白特性的影响[J].食品科学,2012,33(14):273-276.
[24] UTRERA M,ESTEVEZ M. Oxidation of myofibrillar proteins and impaired functionality: Underlying mechanisms of the carbonylation pathway[J]. Journal Agriculture and Food Chemistry, 2012, 60 (32): 8 002-8 011.
[25] WU S J,PAN S K,WANG H B. Effect of trehalose on Lateolabrax japonicus myofibrillar protein during frozen storage[J]. Food Chemistry,2014, 160:281-285.
[26] HERRERA J R, MACKIE I M.Cryoprotection of frozen-stored actomyosin of farmed rainbow trout (Oncorhynchus mykiss) by some sugars and polyols[J]. Food Chemistry, 2004, 84(1): 91-97.
[27] XIONG Y L, AGYARE K K, ADDO K K. Hydrolyzed wheat gluten suppresses transglutaminase-mediated gelation but improves emulsification of pork myofibrillar protein[J]. Meat Science,2008,80(2):535-544.
[28] 吕卫金,赵进,汪金林,等.茶多酚延缓冷藏大黄鱼肌原纤维蛋白变性降解机理研究[J].中国食品学报,2014,14(1):60-67.
[29] 孔保华,刘骞,陈洪生,等.肉制品品质及质量控制[M].北京:科学出版社,2015:39-41.
[30] 白登荣,董唯,齐昕宇,等.γ-聚谷氨酸对鸡肉肌原纤维蛋白功能特性的影响[J].食品与机械,2017,33(9):35-40.
[31] 林静.茶多酚处理对微冻泥鳅保鲜效果的影响[D].重庆:西南大学,2017.
[32] 雷叶斯,杨巨鹏,吕春霞,等.羟自由基氧化体系对大黄鱼肌原纤维蛋白结构的影响[J].食品工业科技,2019,40(1):29-33.
[33] 李银.蛋白氧化对肌肉保水性的影响机制研究[D]. 北京: 中国农业科学院, 2014.
[34] 于建行.泥鳅贮藏保鲜及鱼糜凝胶制备工艺研究[D].重庆:西南大学,2015.
[35] XIONG Y L, BLANCHARD S P, OOIZUMI T,et al. Hydroxyl radical and ferryl-generating systems promote gel network formation of myofibrillar protein[J].Journal of Food Science,2010,75(2): C215-C221.
[36] HWANG J S, LAI K M, HSU K C. Changes in textural and rheological properties of gels from tilapia muscle proteins induced by high pressure and setting[J].Food Chemistry,2007,104(2): 746-753.
[37] 姜晴晴.冻融过程中带鱼脂肪和蛋白氧化及其对肌肉品质影响的研究[D].杭州:浙江大学,2015.
