为提高羊骨的高值利用,研究了木瓜蛋白酶和胃蛋白酶分步酶解提取羊骨中可溶性钙的最佳工艺条件。以羊骨粉为原料,游离钙含量为指标,通过单因素试验和响应面优化2种酶的酶解工艺,并在不同加入顺序和时间组合下进行分步水解,提取羊骨中可溶性钙。结果表明,木瓜蛋白酶和胃蛋白酶的最佳酶解条件分别为酶解温度55、40 ℃,酶解时间均为4 h,酶解pH 6.0、2.5,加酶量5 000、5 300 U/g,所测实际游离钙含量是79.02和1 008.68 mg/100 g;在双酶各自最优酶解条件下,按先木瓜蛋白酶后胃蛋白酶的加入顺序,分别酶解3 h,游离钙含量达到了1 839.14 mg/100 g,极显著高于单酶和其他组合(P<0.01)。
The optimum condition for extracting soluble calcium from sheep bone by papain and pepsin was studied. Using free calcium content as an index, the enzymatic hydrolysis processes of papain and pepsin were optimized by single factor test and response surface analysis, and the two step enzymolysis was carried out under different addition sequences and time combinations of the two enzymes. The results showed that the optimal extraction conditions for papain and pepsin were at 55 ℃, pH 6.0 and at 40 ℃, pH 2.5, hydrolyzed for 4 h with the additions of 5 000 U/g and 5 300 U/g, respectively. Under these conditions, the free calcium contents extracted were 79.02 mg/100 g and 1 008.68 mg/100g, respectively. Based on the optimal hydrolysis conditions of papain and pepsin, the optimum condition for the two-step enzymolysis was to add papain first followed by pepsin and hydrolyzed for 3 h each. Under this condition, the free calcium content reached 1 839.14 mg/100 g, which was significantly higher than those of single enzyme extraction and other combinations (P<0.01). In conclusion, this study intended to lay a foundation for high value utilization of sheep bones and development of related products.
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