藏灵菇中含有丰富的乳酸菌种,其发酵乳具有较高的抗氧化能力。为得到具有高抗氧化能力的菌株,从14份藏灵菇样品中分离出了80株乳酸菌,其中包括30株开菲尔乳杆菌、9株植物乳杆菌、7株保加利亚乳杆菌、3株干酪乳杆菌、5株假肠膜明串珠菌、26株耐久肠球菌。该文从中选出了36株乳酸菌进行4种抗氧化活性测定:DPPH自由基的清除能力、超氧阴离子的清除能力、还原能力、抗脂质过氧化能力,发现完整细胞组M23、M31、M14、M22,破碎细胞组M30、M22、M14、M30有较高抗氧化活性,并测定了8株抗氧化能力优良的菌株发酵乳的抗氧化能力,发现完整细胞组抗氧化能力较高的4株菌,其发酵乳抗氧化能力也较高。
Tibetan kefir is rich in lactic acid bacteria, and its fermented milk has high antioxidant capacity. In order to obtain high antioxidant strains, 80 strains of lactobacillus were isolated from 14 samples of Tibetan kefir, including 30 strains of Lactobacillus kefir, 9 strains of Lactobacillus plantarum, 7 strains of Lactobacillus bulgaricus, 3 strains of Lactobacillus casei, 5 strains of Leuconostoc pseudoenterica and 26 strains of Enterococcus durans. Lactobacillus kefir and Enterococcus durans are dominant strains. 36 strains of lactic acid bacteria were evaluated based on their scavenging capacity of DPPH free radicals, superoxide anions, reducing capacity and anti-lipid peroxidation ability. Among them, M23, M31, M14, M22 in intact cell group and M30, M22, and M14 in broken cell group had higher antioxidant activity. These strains were used to ferment milk and the intact cell group exhibited higher antioxidant ability.
[1] 郑荣梁.自由基生物学[M]. 北京:高等教育出版社,2007.
[2] 张雅楠,梁鹏,谢静仪,等. 天然食品抗氧化剂的研究进展[J]. 中国食品与营养,2019,25(1):68-72.
[3] 赵彤,钟宜科,荀一萍,等. 乳酸菌抗氧化性及其作用机制研究进展[J]. 中国食品添加剂,2018, 175(9):172-179.
[4] 李唤宇,高原,牟光庆,等. 乳酸菌抗氧化活性研究进展[J]. 食品研究与开发, 2019,40(7):194-202.
[5] 杨明阳,田建军,景智波,等. 乳酸菌抗氧化调控体系研究进展[J]. 食品科学,2018,39(15):300-305.
[6] 刘珊春,赵欣,骞宇,等.西藏羊八井地区牦牛酸乳中乳酸菌菌种鉴定[J]. 食品工业科技,2016,37(10):208-212.
[7] 陈芝兰,杨吉霞,李梦寒,等. 西藏地区传统发酵乳中乳酸菌多样性及微生物数量分析[J]. 食品科学,2013,34(17):140-145.
[8] 刘珊春.传统发酵乳中抗氧化乳酸菌的筛选与功能评价[D]. 重庆:西南大学,2016.
[9] 张江巍,曹郁生,李海星,等. 乳酸菌抗氧化活性及检测方法[J]. 中国乳品工业,2005,33(9):53-56.
[10] 杨静秋.抗氧化乳酸菌的筛选及其对氧化损伤的CT-26细胞的保护作用[D]. 无锡:江南大学,2009.
[11] 刘洋,郭宇星,潘道东. 4种乳酸菌体外抗氧化能力的比较研究[J]. 食品科学,2012,33(11):25-29.
[12] 顾品品,邢家溧,王刚,等. 几种体外评价乳酸菌无细胞提取物抗氧化活性方法的比较研究[J]. 食品工业科技,2015,36(23):84-88.
[13] LIN M Y,YEN C L. Antioxidative ability of lactic acid bacteria[J]. Journal of Agricultural and Food Chemistry,1999,47(4):1 460-1 466.
[14] KULLISAAR T,ZILMER M,MIKELSAAR M,et al. Two antioxidative Lactobacilli strains as promising probiotics[J]. International Journal of Food Microbiology,2002,72(3):215-224.
[15] LI S Y,ZHAO Y J,ZHANG L,et al. Antioxidant activity of Lactobacillus plantarum strains isolated from traditional Chinese fermented foods[J]. Food Chemistry,2012,135(3):1 914-1 919.
[16] 米兰,赵小元,张炎,等. 藏灵菇酸乳的抗氧化性研究[J]. 食品科技,2014,39(1):78-83.
[17] 张书文.抗氧化乳酸菌的筛选及其特性研究[D]. 呼和浩特:内蒙古农业大学,2009.
[18] 罗章,陈历水,陈力俊,等.藏灵菇乳中具有优良抗氧化活性乳酸菌的筛选鉴定[J]. 食品科学,2015,36(3):109-113.
[19] 陈孝勇,李键,赵欣,等. 传统发酵牦牛酸乳中益生性乳酸菌的体外筛选[J]. 食品与发酵工业,2016,42(4):85-90.
[20] 余荷.假肠膜明串珠菌致血流感染2例报告[J]. 山东医药,2015,55(40):72-73.
[21] 瞿介明,龚炜,周春妹. 肠球菌耐药性监测[J]. 中华医院感染学杂志,2000,10(5):327-329.
[22] 胡东良.乳酸菌的抗肿瘤、抗变异原及免疫增强作用[J]. 中国乳品工业,1997,25(6):11-14.