食品与发酵工业 >

2019 , Vol. 45 >Issue 22: 32 - 38

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.021267

研究报告

泡菜源屎肠球菌Enterococcus faecium R2的环境胁迫耐受性及安全性评价

  • 陈志娜 ,
  • 裴纪柳 ,
  • 叶韬 ,
  • 薛咏振 ,
  • 詹志强 ,
  • 李雅 ,
  • 刘天
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  • 1 (淮南师范学院 生物工程学院,安徽 淮南,232038)
    2 (资源与环境生物技术安徽普通高校重点实验室,安徽 淮南,232038)
博士,讲师(本文通讯作者,Email: chenzhina0625@163.com)。

收稿日期: 2019-06-03

  网络出版日期: 2020-02-16

基金资助

安徽省自然科学基金青年项目(1908085QC122);淮南市科技局科技计划项目(2018A365);淮南师范学院自然科学研究重点项目(2017xj03zd);淮南师范学院博士科研启动金(58886)

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Environmental stress tolerance and safety evaluation of Enterococcus faecium R2 isolated from pickles

  • CHEN Zhina ,
  • PEI Jiliu ,
  • YE Tao ,
  • XUE Yongzhen ,
  • ZHAN Zhiqiang ,
  • LI Ya ,
  • LIU Tian
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  • 1 (College of Biological Engineering, Huainan Normal University, Huainan 232038, China)
    2 (Key Laboratory of Bioresource and Environmental Biotechnology of Anhui Higher Education Institutes, Huainan 232038, China)

Received date: 2019-06-03

  Online published: 2020-02-16

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摘要

屎肠球菌R2是分离于泡菜中的优势产酸菌,可高效利用豆腐黄浆水中的低聚糖产酸制备酸浆,可作为一种新型酸浆纯种发酵剂。该文通过毒力基因检测、万古霉素耐药基因筛查、有害代谢产物实验来评价屎肠球菌R2的安全性,并进一步研究该菌株对外界环境的耐受性,为生产一种新型酸浆纯种发酵剂奠定基础并为其实际生产应用提供科学基础。结果表明,屎肠球菌R2的毒力基因检测和万古霉素耐药基因筛查及有害代谢产物检测结果均为阴性,也不会产生溶血现象;该菌株对外界环境中的酸性、胆盐及高渗透压耐受性较好且具有一定的自聚集性和疏水性。由此可见屎肠球菌R2具有安全性。

关键词： 屎肠球菌; 安全性; 毒力基因; 万古霉素耐药基因; 环境胁迫耐受性

本文引用格式

陈志娜 , 裴纪柳 , 叶韬 , 薛咏振 , 詹志强 , 李雅 , 刘天 . 泡菜源屎肠球菌Enterococcus faecium R2的环境胁迫耐受性及安全性评价[J]. 食品与发酵工业, 2019 , 45(22) : 32 -38 . DOI: 10.13995/j.cnki.11-1802/ts.021267

Abstract

Enterococcus faecium R2, which can efficiently ferment bean curd water and produce acid, is a dominant acid-producing bacterium in the pickles fermentation process. It has been considered as a new acid slurry pure starter. In this study, the safety and the environmental tolerance of E. faecium R2 were evaluated. E. faecium R2 shown negative results on tests of virulence factors, resistance genes and harmful metabolites, as well as no hemolytic activity was detected. It exhibited tolerance to acidity, bilt salt and high osmotic pressure in external environments. Additionally, self-aggregation and hydrophobicity of E. faecium R2 were observed during the study. Therefore, E. faecium R2 can be considered as safe.

Key words： Enterococcus faecium; safty; virulence genes; vancomycin resistant genes; environmental stresses tolenrance

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