食品与发酵工业 >

2019 , Vol. 45 >Issue 22: 83 - 89

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.021480

研究报告

酶转化三七茎叶总皂苷制备稀有人参皂苷C-K

  • 王彩艳 ,
  • 刘春莹 ,
  • 肖永坤 ,
  • 左康泽 ,
  • 陈双 ,
  • 徐龙权 ,
  • 宋建国 ,
  • 鱼红闪
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  • 1 (大连工业大学 生物工程学院,辽宁 大连,116034)
    2 (大连大学,辽宁 大连,116622)
    3 (葵花药业集团(天津)药物研究院,天津,300457)
硕士研究生(鱼红闪教授为通讯作者,E-mail: hongshan@dlpu.edu.cn)。

收稿日期: 2019-06-26

  网络出版日期: 2020-02-16

基金资助

国家科技重大专项“重大新药创制”项目(2012ZX095 03001-003);国家高端外国专家项目(GDT20152100019)

收起

Enzymatic production of minor ginsenoside C-K from notoginseng stem-leaf ginsenoside

  • WANG Caiyan ,
  • LIU Chunying ,
  • XIAO Yongkun ,
  • ZUO Kangze ,
  • CHEN Shuang ,
  • XU Longquan ,
  • SONG Jianguo ,
  • YU Hongshan
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  • 1 (Dalian Polytechnic University, Dalian 116034, China)
    2 (Dalian University, Dalian 116622, China)
    3 (Tianjin Pharm Institute of Sunflower Pharm Co. Ltd, Tianjin 300457, China)

Received date: 2019-06-26

  Online published: 2020-02-16

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摘要

为了提高三七茎叶总皂苷的利用率和C-K酶转化得率,采用Aspergillus niger sp.G8菌所产酶与Aspergillus niger sp.G4菌所产酶的等体积混合酶(以下简称G8-G4混合酶),研究了酶转化三七茎叶总皂苷进而制备C-K的方法。结果表明,市销的三七茎叶总皂苷中,主要成分是人参二醇类皂苷,其中含量最多的皂苷是Rb3、C-Mx1、Rc和Fc,质量分数分别为26.38%、14.28 %、12.73%、10.25%。G8-G4混合酶的最佳反应条件为45 ℃、pH 5.0、底物质量浓度25 g/L、反应24 h。三七茎叶总皂苷经G8-G4混合酶转化后得到4种产物为C-K、C-Mx、Fc、R7,其中C-K的转化得率为32.7%。

关键词： 三七茎叶总皂苷; 酶转化; 人参皂苷C-K; 人参皂苷C-Mc

本文引用格式

王彩艳 , 刘春莹 , 肖永坤 , 左康泽 , 陈双 , 徐龙权 , 宋建国 , 鱼红闪 . 酶转化三七茎叶总皂苷制备稀有人参皂苷C-K[J]. 食品与发酵工业, 2019 , 45(22) : 83 -89 . DOI: 10.13995/j.cnki.11-1802/ts.021480

Abstract

In order to improve the utilization rate of total saponins of Panax notoginseng and the related conversion rate to C-K, mixed enzymes produced by Aspergillus niger G8 and G4 strains were used to transform the total stem-leaf saponin from P. notoginseng to C-K. The results showed that the main component of commercially available notoginseng stem-leaf ginsenosides are propanediol type. In which the content of Rb3, C-Mx1, Rc and Fc was 26.38%, 14.28%, 12.73%, and 10.25%, respectively. The optimum enzymatic transformation was obtained after 24 h reaction at 45 ℃ and pH 5.0 using 2.5% substrate . Four products, C-K, C-Mx, Fc and R7 were obtained, of which 32.7% was C-K.

Key words： notoginseng stem-leaf ginsenoside; enzyme biotransformation; ginsenoside C-K; ginsenoside C-Mc

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