为了提高牛类芽孢杆菌BD3526发酵乳中α-葡萄糖苷酶抑制剂的产量,采用单因素实验和响应面法对发酵参数进行优化。BD3526合成α-葡萄糖苷酶抑制剂的优选参数为：培养时间43 h、培养温度29 ℃、脱脂乳质量浓度为5 g/100 g(水)、三角瓶装量为50 mL/250 mL。在此条件下,BD3526发酵乳对α-葡萄糖苷酶的半数抑制浓度IC50为15.1 mg/mL,与理论值(14.6 mg/mL)基本一致,比优化前(22.4 mg/mL)降低了33%。该研究为α-葡萄糖苷酶抑制剂的后续分离工作提供了保障,也为其他类似的研究提供了理论依据。
This study was constructed to increase the yield of α-glucosidase inhibitor in fermented milk by Paenibacillus bovis BD3526. The fermentation parameters were optimized by single-factor experiments and response surface method. The results showed that the optimal condition for BD3526 to synthesize α-glucosidase inhibitor was as follows: cultivated at 29 ℃ for 43 h in a 50 mL medium with 5 g/100 g H2O skimmed milk in a 250 mL flask. Under the optimized condition, the IC50 of BD3526 fermented milk to inhibit α-glucosidase was 15.1 mg/mL, which agreed with the predicted value (14.6 mg/mL) and was 33% lower than that before optimization. In conclusion, this study investigated the optimized process of preparing α-glucosidase inhibitor in fermented skimmed milk by microorganisms for the first time, which provides a guarantee for subsequent separation of α-glucosidase inhibitor and also lays a foundation for other similar studies.
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