以玉米蛋白和大豆蛋白为原料,按m(玉米蛋白)∶m(大豆蛋白)=7∶3复配后,研究其水解产物的抗氧化活性。首先用米曲霉(Aspergillus oryzae)种曲和碱性蛋白酶(alcalase)协同水解玉米-大豆蛋白复配物,制备玉米-大豆蛋白水解物(CSPHs);然后通过测定CSPHs的DPPH自由基清除率和金属离子(Fe2+)螯合能力,分析其体外抗氧化活性;再建立双氧水(H2O2)氧化应激模型,探讨CSPH的细胞抗氧化活性。结果表明,CSPHs在体外具有较强的抗氧化活性,并且在加入质量分数为10%的米曲霉种曲与加入质量分数0.8%的碱性蛋白酶情况下,协同水解后的产物CSPH4具有良好的细胞抗氧化活性。此时的水解度、可溶性蛋白含量分别为46.10%和(73.04±1.68) mg/mL,·DPPH清除率和亚铁离子螯合能力分别为(41.26±0.69)%和(50.23±3.15)%。CSPH4可降低H2O2诱导的氧化应激人结肠癌细胞(Caco-2)细胞内活性氧(reactive oxygen species, ROS)的含量。米曲霉种曲和Alcalase协同水解复配物得到的产物具有良好的抗氧化活性,具有运用到食品工业中作为抗氧化剂的潜力。
Using corn gluten meal and soy protein as raw materials, the antioxidant activity of the hydrolysate was studied after the corn protein: soy protein content ratio was 7∶3. Firstly, Aspergillus oryzae and Alcalase synergistic hydrolysis of the mixture of corn protein and soy protein to prepare corn soy protein hydrolysate(CSPHs); then the DPPH free radical scavenging rate and metal ion (Fe2+) chelation ability of CSPHs were determined to analyze its antioxidant activity in vitro. Establish a model of oxidative stress of hydrogen peroxide (H2O2) to investigate the cellular antioxidant activity of CSPH. The results showed that CSPHs had strong antioxidant activity in vitro, and the synergistically hydrolyzed product CSPH4 had good cellular antioxidant activity when 10% mass fraction of Aspergillus oryzae was added and 0.8% mass fraction of Alcalase was added, the hydrolysis degree and soluble protein content of the hydrolysate were 46.10% and (73.04±1.68) mg/mL, the scavenging capacities for DPPH radical and the chelating capacity of ferrous ions of the hydrolysate were (41.26±0.69)% and (50.23±3.15)%, respectively. CSPH4 could reduce the content of ROS in Caco-2 cells induced by H2O2-induced oxidative stress. The products obtained by the synergistic hydrolysis of Aspergillus oryzae and Alcalase have excellent antioxidant activity and intracellular ROS scavenging ability, which has the potential to be used as an antioxidant in the food industry.
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