贮运与保鲜

低温贮藏期间梨果皮酚类物质及抗氧化性变化

  • 黄怡 ,
  • 高春丽 ,
  • 毕阳 ,
  • 李冬美 ,
  • 董玉鹏 ,
  • 张彦东 ,
  • 李永才
展开
  • 甘肃农业大学 食品科学与工程学院,甘肃 兰州,730070

修回日期: 2019-06-19

  网络出版日期: 2019-11-15

基金资助

梨果皮绿原酸介导的Alternaria alternata活性氧代谢对其侵染的调控(3146053)

Changes in phenolic compounds and antioxidant activity of pear peel during cold storage

  • HUANG Yi ,
  • GAO Chunli ,
  • BI Yang ,
  • LI Dongmei ,
  • DONG Yupeng ,
  • ZHANG Yandong ,
  • LI Yongcai
Expand
  • College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China

Revised date: 2019-06-19

  Online published: 2019-11-15

摘要

为了研究甘肃省主要梨果低温贮藏期间果皮酚类物质含量及其抗氧化性的变化,在苹果梨、早酥梨和皇冠梨果皮总酚、总黄酮测定的基础上,采用HPLC法分析8种主要酚类物质含量;并通过FRAP、DPPH和ABTS+·法评价不同时期酚类提取物的抗氧化能力,及其与酚类物质含量的相关性。8种主要酚类物质中熊果苷含量最高,其在苹果梨、早酥梨和皇冠梨果皮的平均含量分别达到10.22、16.92和11.17 mg/g FW;除早酥梨果皮中儿茶素和苹果梨、皇冠梨中芦丁含量在低温贮藏期间呈升高趋势外,总酚、总黄酮及其他主要酚类物质均呈下降趋势;果皮酚类提取物的抗氧化能力随贮藏期的延长而下降,且与总酚、总黄酮、阿魏酸含量呈显著正相关(P<0.05)。梨果皮酚类物质含量及特性因品种、贮藏期而异,该研究为进一步揭示梨果皮酚类物质的变化规律及其开发利用提供理论依据。

本文引用格式

黄怡 , 高春丽 , 毕阳 , 李冬美 , 董玉鹏 , 张彦东 , 李永才 . 低温贮藏期间梨果皮酚类物质及抗氧化性变化[J]. 食品与发酵工业, 2019 , 45(19) : 219 -226 . DOI: 10.13995/j.cnki.11-1802/ts.021147

Abstract

This study aimed to investigate the changes of phenolic compounds and antioxidant capacity of peel phenol extracts of main pear cultivars in Gansu province during cold storage. 8 main phenolic compounds in the peel of Pingguoli, Zaosu and Huangguan pear were analyzed by HPLC method based on determining total phenols and flavonoids content. Antioxidant activities of phenolic extracts from pear peels were evaluated by the FRAP, DPPH and ABTS+· antioxidant indexes. The correlation between the content of phenolic compounds and 3 antioxidant indexes were also analyzed. The results indicated that the arbutin was the highest among 8 main phenolics, and the average contents of arbutin in peel of Pingguoli, Huangguan and Zaosu pear were 10.22, 16.92 and 11.17 mg/g, respectively. In addition, the content of total phenols, and total flavanoids and the main content of phenolics decreased, while the catechin in Zaosu pear and rutin in Pingguoli pear as well as Huangguan pear increased. The antioxidant capacity of phenolic extracts showed a decreasing trend, and there was a significant positive correlation with the content of total phenols, total flavonoids and ferulic acid (P<0.05). It is suggested that the phenolic compounds in pear peels varies from pear cultivars and storage time, and provides a theoretical basis for further elucidating dynamic change rules of phenolic compounds in pear peel and their comprehensive utilization.

参考文献

[1] 吴小华, 颉敏华,陈柏,等. GA_3处理对黄冠梨采后常温贮藏品质变化及病害发生的影响[J]. 甘肃农业科技, 2019(3): 11-17.
[2] 孙琰, 马捷,张静荣,等. 三个主栽白梨品种鲜切后品质及微生物变化的比较[J]. 食品与发酵科技, 2018, 54(2): 41-47;86.
[3] JIMÉNEZ-AGUILAR D, MÚJICA-PAZ H, WELTI-CHANES J. Phytochemical characterization of prickly pear (Opuntia spp) and of its nutritional and functional properties: a review[J]. Current Nutrition & Food Science, 2014, 10(1): 57-69.
[4] KOLNIAK-OSTEK J, OSZMIANSKI J.Characterization of phenolic compounds in different anatomical pear (Pyrus communis L) parts by ultra-performance liquid chromatography photodiode detector-quadrupole/time of flight-mass spectrometry (UPLC-PDA-Q/TOF-MS)[J]. International Journal of Mass Spectrometry, 2015, 392: 154-163.
[5] SOTO-VACA A, GUTIERREZ A, LOSSO J N, et al.Evolution of phenolic compounds from color and flavor problems to health benefits[J]. Journal of Agricultural and Food Chemistry, 2012, 60(27): 6 658-6 677.
[6] 夏乐晗, 陈玉玲,冯义彬,等. 不同品种杏果实发育过程中类黄酮、总酚和三萜酸含量及抗氧化性研究[J]. 果树学报, 2016, 33(4): 425-435.
[7] JARAMILLO S, LOPEZ S, VARELA L M, et al.The flavonol isorhamnetin exhibits cytotoxic effects on human colon cancer cells[J]. Journal of Agricultural and Food Chemistry, 2010, 58(20): 10 869-10 875.
[8] 郭海丽. 梨皮化学成分的LC-MS和NMR研究[D]. 武汉:华中师范大学, 2014.
[9] 李丽梅, 赵哲,何近刚,等. 不同品种梨果实酚类物质和抗氧化性能分析[J]. 食品科学, 2014, 35(17): 83-88.
[10] 李磊, 寇晓虹. 黄冠梨储藏期间果皮酚类物质代谢与鸡爪病发生的关系[J]. 河南工业大学学报(自然科学版), 2011, 32(4): 74-79.
[11] RIVERO R M, RUIZ J M, GARCÍA P C, et al. Resistance to cold and heat stress: accumulation of phenolic compounds in tomato and in watermelon plants[J]. Plant Science, 2001, 160(2): 315-321.
[12] 周广志, 鲁敏,安华明. 刺梨果实发育过程中主要活性物质含量及其抗氧化性分析[J]. 食品科学, 2018, 39(22): 20-25.
[13] GUAN J F, HE J G, SHEN C G, et al.Chapter 17-how cultivars influence fruit composition: total phenols, flavonoids contents, and antioxidant activity in the pulp of selected Asian pears[M]. Processing and Impact on Active Components in Food, 2015:139-145.
[14] KOLNIAK-OSTEK J.Content of bioactive compounds and antioxidant capacity in skin tissues of pear[J]. Journal of Functional Foods, 2016, 23: 40-51.
[15] 翟锐, 房晨,范二婷,等. ‘早酥’梨及其红皮芽变‘红早酥’梨类黄酮组分变化与合成模式研究[J]. 果树学报, 2016, 33(S1): 75-82.
[16] 李磊, 谢冰,寇晓虹. 高效液相色谱-串联质谱测定黄冠梨果皮中主要酚类物质[J]. 食品工业, 2012, 33(5): 132-135.
[17] SINGLETON V L, ORTHOFER R, LAMUELA-RAVENTóS R M. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent[J]. Methods in Enzymology, 1999, 299: 152-178.
[18] DEWANTO V, WU X, ADOM K K, et al.Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity[J]. Journal of Agricultural and Food Chemistry, 2002, 50(10): 3 010-3 014.
[19] JIA Z S, TANG M C, WU J M.The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals[J]. Food Chemistry, 1999, 64(4): 555-559.
[20] LOCATELLI M, GINDRO R, TRAVAGLIA F, et al.Study of the DPPH-scavenging activity: development of a free software for the correct interpretation of data[J]. Food Chemistry, 2009, 114(3): 889-897.
[21] RE R, PELLEGRINI N, PROTEGGENTE A, et al.Antioxidant activity applying an improved ABTS radical cation decolorization assay[J]. Free Radical Biology and Medicine, 1999, 26(9-10): 1 231-1 237.
[22] BENZIE I F, STRAIN J J.The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay[J]. Analytical Biochemistry, 1996, 239(1): 70-76.
[23] CHENG Y, LIU L, ZHAO G, et al.The effects of modified atmosphere packaging on core browning and the expression patterns of PPO and PAL genes in “Yali” pears during cold storage[J]. LWT-Food Science and Technology, 2015, 60(2): 1 243-1 248.
[24] OCHOA-VELASCO C E, GUERRERO-BELTRAN J A. The effects of modified atmospheres on prickly pear (Opuntia albicarpa) stored at different temperatures[J]. Postharvest Biology and Technology, 2016, 111: 314-321.
[25] 张小双, 郑迎春,曹玉芬,等. ‘早酥’和‘南果梨’16个部位多酚物质组成及含量分析[J]. 中国农业科学, 2017, 50(3): 545-555.
[26] 毕阳, 郭玉蓉,李永才,等. 冷藏期间三种梨果皮中酚类物质含量及多酚氧化酶活性变化与褐变度的关系[J]. 制冷学报, 2002(4): 52-54.
[27] LI D, CHENG Y, DONG Y, et al.Effects of low temperature conditioning on fruit quality and peel browning spot in “Huangguan” pears during cold storage[J]. Postharvest Biology and Technology, 2017, 131: 68-73.
[28] CHENG Y D, LIU L Q, FENG Y X, et al.Effects of 1-MCP on fruit quality and core browning in “Yali” pear during cold storage[J]. Scientia Horticulturae, 2019, 243: 350-356.
[29] KOU X, WU M, LI L, et al.Effects of CaCl2 dipping and pullulan coating on the development of brown spot on “Huangguan” pears during cold storage[J]. Postharvest Biology and Technology, 2015, 99: 63-72.
[30] 商叶, 汪晓谦,刘洋,等. 采后PDJ处理对‘南果梨’果实酚类物质含量变化的影响[J]. 中国果树, 2017, (S1): 66-69;86.
[31] DAI J, MUMPER R J.Plant phenolics: extraction, analysis and their antioxidant and anticancer properties[J]. Molecules, 2010, 15(10): 7 313-7 352.
[32] SCALBERT A, MANACH C, MORAND C, et al.Dietary polyphenols and the prevention of diseases[J]. Critical Reviews in Food Science and Nutrition, 2005, 45(4): 287-306.
[33] SILVA F G D, O’CALLAGAHAN Y, O’BRIEN N M, et al. Antioxidant capacity of flaxseed products: the effect of in vitro digestion[J]. Plant Foods for Human Nutrition, 2012, 68(1): 24-30.
[34] MANGANARIS G A, GOULAS V, VICENTE A R, et al.Berry antioxidants: small fruits providing large benefits[J]. Journal of the Science of Food and Agriculture, 2014, 94(5): 825-833.
[35] DEBHI F, HASIB A, BOUAZIZ M, et al.Effects of phenolic compounds and betalain pigments on the antioxidant capacity of Moroccan prickly pear juices[J]. Nature & Technology, 2013, 9: 2-7.
[36] AWIKA J M, ROONEY L W, WU X, et al.Screening methods to measure antioxidant activity of sorghum (Sorghum bicolor) and sorghum products[J]. Journal of Agricultural and Food Chemistry, 2003, 51(23): 6 657-6 662.
[37] LI X, WANG T, ZHOU B, et al.Chemical composition and antioxidant and anti-inflammatory potential of peels and flesh from 10 different pear varieties (Pyrus spp)[J]. Food Chemistry, 2014, 152: 531-538.
[38] CHU Y H, CHANG C L, HSU H F.Flavonoid content of several vegetables and their antioxidant activity[J]. Journal of the Science of Food & Agriculture, 2000, 80(5): 561-566.
[39] DU G, LI M, MA F, et al.Antioxidant capacity and the relationship with polyphenol and vitamin C in Actinidia fruits[J]. Food Chemistry, 2009, 113(2): 557-562.
文章导航

/