从云南大理市弥渡县石夹泉热泉的55 ℃底泥中筛选到1株高温脂肪酶的高产菌株,进行显微形态及生理生化特征、16S rRNA基因序列分析,将其初步鉴定为不动杆菌属(Acinetobacter sp.)的一株菌,命名为Acinetobacter sp. Lip-55。对其生长条件及酶学性质进行了研究,结果表明,该菌株耐高温性较好,菌株在55 ℃仍能生长,菌株最适生长温度为37 ℃。所产脂肪酶最适酶活温度为55℃,最适反应pH值为5.0。该酶在25 ℃以下,能保持良好的稳定性,Zn2+、Ca2+、Mg2+对该酶有一定的抑制作用,Mn2+、K+、Fe2+、Cu2+均对该酶活力起到促进作用,其中Fe2+的促进效果最为显著。
A strain Lip-55 producing thermostable lipase was isolated from the sediment of Shijiaquan hot spring. It was identified as Acinetobacter sp. According to its morphology, physiological and biochemical characteristics, and 16S rRNA sequence analysis. The growth conditions and enzymatic properties were studied. The results showed that it could grow at 55 ℃ and the optimum growth temperature was 37 ℃. The lipase had maximum activity at 55 ℃ and t pH 5.0. Zn2+, Ca2+ and Mg2+ had a certain inhibitory effect on the enzyme activity. Mn2+, K+, Fe2+ and Cu2+ promoted the activity, of which Fe2+ was the most obvious.
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