为筛选得到降解白酒酒糟中纤维素能力较强的菌株,该研究以酒醅和窖泥为研究对象,通过富集、分离,并以CMC-Na为唯一碳源初筛得到9株产纤维素酶菌株,菌株P7 D/d值最大,达到23;经复筛,菌株P7 CMC酶活最高,为49.31 U,在4 d将滤纸条完全降解,酒糟降解率为22.1 %。不同发酵条件对酶活的影响研究表明,菌株P7和P′16对酒糟利用较好,最适培养时间在48 h左右,在初始pH 4.0和44 ℃下酶活高于其他菌株。对菌株P7和P′16进行生理生化鉴定,结合16S rDNA测序对比,菌株P7为贝莱斯芽孢杆菌,P′16为解淀粉芽孢杆菌。
The purpose of this study is to screen strains that have strong cellulose degradation ability for the degrading distiller's grains. From the wine cellar and pit mud, 9 strains of microorganisms that produce cellulase were obtained using sodium carboxymethyl cellulose as the sole carbon source. The strain P7 had the highest D/d value (23) and produced the highest cellulase of 49.31 IU/mL. After 4 days of incubation, the filter paper was completely degraded, and the degradation rate of distiller's grains was 22.1%. In addition, the effect of different fermentation conditions on enzyme activity were studied. The strains P7 and P'16 had the highest performance in distiller's grains degradation. The optimum culture time was about 48 h. The enzyme activity of the two strains was higher than other strains at initial pH of 4.0 and temperature of 44 ℃. Using physiological and biochemical identification, combining with 16S rDNA sequencing, the strain P7 was identified to be Bacillus brevis, and P'16 to be B. amyloliquefaciens.
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