该文探讨桑椹贮藏年份与5-羟甲基糠醛(5-HMF)含量的相关性。收集贮藏了1、2、3、5年的桑椹样品,日光下观察色泽,烘干法测定水分,高效液相色谱法(HPLC)测定5-HMF的含量。结果表明,桑椹呈现紫黑色,样品之间差异不明显,水分含量为6.35%~15.43%(质量分数)。贮藏1年和2年的共4批样品中5-HMF含量均低于检测限(2.914×10-4 μg)或定量限(9.925×10-4 μg)。贮藏3年的9批样品中5-HMF含量较高,含量为3.18~23.64 μg/g。贮藏5年的2批样品中5-HMF含量最高,分别为74.60 μg/g和83.78 μg/g。桑椹的色泽不能反映贮藏的年份,水分含量与色泽、贮藏年份无关,但5-HMF含量随着贮藏年份的延长从无到有、从低到高,呈现快速上升趋势,具有显著的相关性。该研究为评价和判断桑葚贮藏年份提供基础研究数据。
This study aims at exploring the correlation between storing-year of Mori Fructus (MF) and its 5-hydroxymethylfurfural (5-HMF) content. MF samples stored respectively for 1, 2, 3 and 5 years were collected to observe their color under sunlight, measure their water content by oven-drying method and determine their 5-HMF content by HPLC method. Results showed that the color of MF was purple in black with no obvious variation between samples, that the mass fraction of water content ranged from 6.35% to 15.43%. Among all samples of which the storing year could be clearly defined, the 5-HMF contents in a total 4 batches of MF samples stored for 1 or 2 years were below the limit of detection (2.914×10-4 μg) or limit of quantitation (9.925×10-4 μg), the 5-HMF contents of 9 batches of MF samples stored for 3 years were higher, ranging from 3.180 μg/g to 23.64 μg/g; while the 5-HMF contents of 2 batches of MF samples stored for 5 years were the highest, respectively 74.60 μg/g and 83.78 μg/g. The color of MF cannot indicate the storing year, and the water content was irrelevant to the color or the storing-year of MF; However, the 5-HMF content showed a sharp increase from zero, with significant correlation with storing-year. As the first study on the correlation between storing-year and chemical components of dark-colored Chinese medicinal materials, this study has provided basic research data for the evaluation and determination of storing-year.
[1] 国家药典委员会. 中华人民共和国药典:一部[S]. 北京:中国医药科技出版社, 2015:300.
[2] 钮成拓,李正学,范林旭,等. 桑葚果酒发酵过程中功能性物质的检测及其变化情况[J]. 食品与发酵工业, 2019,45(3):83-88.
[3] 崔素芬,廖芬,何全光,等. 采用离心喷雾干燥技术制备桑葚果粉[J]. 食品与发酵工业, 2012, 38(11):73-78.
[4] 邓娜娜,马永昆,张龙,等. 不同杀菌处理桑椹果醋香气质量的主成分分析[J]. 食品与发酵工业, 2014, 40(4):172-177.
[5] 李春英. 更年期综合征的中医治疗[J]. 中国中医基础医学杂志, 2002, 10(8):57-62.
[6] 郎海燕,陈信义,杨文华. 缺铁性贫血中医药防治康复一体化专家共识[J]. 中华中医药杂志, 2018, 33(8):3 487-3 492.
[7] 胡佑志. 桑葚芝麻蜂蜜膏治少白头[J]. 蜜蜂杂志, 2018, 38(12):38.
[8] 孔凡刚,张丽,巩丽丽,等. DPPH法测定桑椹不同极性部位的抗氧化活性[J]. 山东中医杂志, 2015, 34(11):865-868.
[9] 朱翠玲,陈铭,汪孟涵,等. 桑葚提取物体外抗炎作用及机制的研究[J]. 现代食品科技, 2017, 33(4):61-66.
[10] 尹爱武,田润,邓胜国,等. 桑椹多糖对急慢性酒精中毒小鼠的护肝作用[J]. 现代食品科技, 2016, 32(12):13-19.
[11] 杨美莲,樊志峰,蔡圣宝,等. 桑葚花色苷提取工艺优化及抗氧化活性研究[J]. 云南民族大学学报(自然科学版), 2019, 28(1):9-15.
[12] 陈诚,王柳萍,辛华. 桑椹的质量分析方法研究[J]. 广西中医药, 2016, 39(5):396-399.
[13] 刘洋,周玉,肖会敏,等. HPLC法测定桑椹颗粒中绿原酸的含量[J]. 中医药导报, 2017, 23(1):63-65.
[14] 吴翠,马玉翠,巢志茂. 龙眼肉理化指标与两类贮藏库温度积的动态分析[J]. 中国中医基础医学杂志, 2017, 23(8):1 158-1 161.
[15] 吴翠,马玉翠,晋文慧,等. 简易库和冷藏库贮藏中牛膝理化指标的月动态变化研究[J]. 中国药学杂志, 2017, 52(24):2 151-2 156.
[16] 于莉,吴晓毅,梁曜华,等. 山萸肉不同仓储时间与5-羟甲基糠醛含量的相关性研究[J]. 中国中医药信息杂志, 2015, 22(6):95-98.
[17] 吴翠,高岳瑞,巢志茂,等. 五味子中5-羟甲基糠醛含量与仓储和色泽的相关性[J]. 中国实验方剂学杂志, 2016, 22(15):24-27.
[18] 王超,王淳,李青,等. Maillard反应在中药领域的研究进展[J]. 中国药房, 2011, 22(11):1 038-1 040.
[19] WANG M Y, ZHAO F M, PENG H Y, et al. Investigation on the morphological protective effect of 5-hydroxymethylfurfural extracted from wine-processed fructus corni on human L02 hepatocytes [J]. Journal of Ethnopharmacology, 2010, 130(2):424-428.
[20] 梅国栋,赵玲,陈建平,等. 5-HMF抑制A375细胞增殖的信号转导通路[J]. 中国科技论文, 2014, 9(9):1 005-1 008.
[21] ULBRICHT R J, NORTHUP S J, THOMAS J A. A review of 5-hydroxymethylfurfural (HMF) in parenteral solutions [J]. Fundamental and Applied Toxicology, 1984, 4(5):843-853.
[22] HARDT-STREMAYR M, MATTIOLI S, GREILBERGER J, et al. Determination of metabolites of 5-hydroxymethylfurfural in human urine after oral application [J]. Journal of Separation Science, 2013, 36(4):670-676.