该文探讨桑椹贮藏年份与5-羟甲基糠醛(5-HMF)含量的相关性。收集贮藏了1、2、3、5年的桑椹样品,日光下观察色泽,烘干法测定水分,高效液相色谱法(HPLC)测定5-HMF的含量。结果表明,桑椹呈现紫黑色,样品之间差异不明显,水分含量为6.35%~15.43%(质量分数)。贮藏1年和2年的共4批样品中5-HMF含量均低于检测限(2.914×10-4 μg)或定量限(9.925×10-4 μg)。贮藏3年的9批样品中5-HMF含量较高,含量为3.18~23.64 μg/g。贮藏5年的2批样品中5-HMF含量最高,分别为74.60 μg/g和83.78 μg/g。桑椹的色泽不能反映贮藏的年份,水分含量与色泽、贮藏年份无关,但5-HMF含量随着贮藏年份的延长从无到有、从低到高,呈现快速上升趋势,具有显著的相关性。该研究为评价和判断桑葚贮藏年份提供基础研究数据。
This study aims at exploring the correlation between storing-year of Mori Fructus (MF) and its 5-hydroxymethylfurfural (5-HMF) content. MF samples stored respectively for 1, 2, 3 and 5 years were collected to observe their color under sunlight, measure their water content by oven-drying method and determine their 5-HMF content by HPLC method. Results showed that the color of MF was purple in black with no obvious variation between samples, that the mass fraction of water content ranged from 6.35% to 15.43%. Among all samples of which the storing year could be clearly defined, the 5-HMF contents in a total 4 batches of MF samples stored for 1 or 2 years were below the limit of detection (2.914×10-4 μg) or limit of quantitation (9.925×10-4 μg), the 5-HMF contents of 9 batches of MF samples stored for 3 years were higher, ranging from 3.180 μg/g to 23.64 μg/g; while the 5-HMF contents of 2 batches of MF samples stored for 5 years were the highest, respectively 74.60 μg/g and 83.78 μg/g. The color of MF cannot indicate the storing year, and the water content was irrelevant to the color or the storing-year of MF; However, the 5-HMF content showed a sharp increase from zero, with significant correlation with storing-year. As the first study on the correlation between storing-year and chemical components of dark-colored Chinese medicinal materials, this study has provided basic research data for the evaluation and determination of storing-year.
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