To explore the difference of microbial community structure and its influencing factors in different parts of mechanical and artificial Daqu. Finished mechanical and artificial Daqu were taken as the research objects, their physicochemical and biochemical indexes were measured, and the microbial community diversity of each part was studied by high-throughput sequencing. The correlation between core microbes and physicochemical and biochemical indexes was analyzed by redundancy analysis in two kinds of Daqu. The results showed that the changes in physicochemical and biochemical indexes of different parts of the mechanical and artificial Daqu were the same, while there were some differences in the fungal composition. Similarly, the bacterial composition of different parts of mechanical Daqu was quite different, while that of different parts of artificial Daqu was quite similar. The main different microbes in the two kinds of Daqu were Aspergillus, Geotrichum, Saccharomycopsis, Dipodascus, Thermoactinomyces and Staphylococcus. Among the core microbes, Thermomyces and Thermoactinomyces were mainly distributed in the JX, JB, CX and CB, while Bacillus, Issatchenkia, and Wickerhamomyces were mainly distributed in the JP, JZ, CP and CZ. Redundancy analysis showed that moisture, acidity, reducing sugar and amino acid nitrogen were the main environmental factors affecting two kinds of Daqu microbes, and Bacillus, Rhizomucor, Trichosporon, Saccharomycopsis, Candida and Issatchenkia were the main microbes affecting the function of Daqu.
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