植物乳杆菌微胶囊可以增加菌体对不利条件的抵抗力,从而减少其活性损失。传统方法制备的微胶囊存在粒径分布不均匀、形状不规则等问题,而微流控技术可以精确控制微胶囊的大小,可通过高度阵列化的微通道来提高微囊化产量。该文设计了集成多个微流体液滴生成器的阵列芯片,以液滴直径大小、变异系数及液滴生成频率为评价指标,比较了2种流体分布层结构以及2种液滴生成器模式对液滴生成的影响。结果表明,树状分布的微通道间压力较平衡,更能实现流体的均匀输送;圆形阵列更有利于单分散液滴的形成。选择树状分布的圆形阵列芯片应用于植物乳杆菌的包埋,当流速比值为15时,液滴生成频率为20.3 Hz,包埋率为96.4%,实现了植物乳杆菌的高效率封装和受控释放。该研究为高通量制备益生菌微胶囊提供了新思路。
Lactobacillus plantarum microcapsules can increase the resistance of the bacteria to adverse conditions, thereby reducing their loss of activity. Microcapsules prepared by traditional methods have problems such as uneven particle size distribution and irregular shape, while microfluidic technology has the characteristics of precise control of the size and shape of microcapsules, and can improve microencapsulation output through highly arrayed microchannels. In this paper, array chips integrating multiple MFDGs were designed. The effects of two fluid distribution layer structures and two droplet generator modes on droplet generation were compared. The droplet diameter, coefficient of variation and droplet generation frequency were used as evaluation indicators. The results showed that the pressure between the microchannels distributed in the tree-like distribution is more balanced, and the fluid can be transported more uniformly; the circular array is more conducive to the formation of monodisperse droplets. The circular array chip with tree-like distribution was selected for the embedding of L. plantarum. When the flow rate ratio was 15, the droplet generation frequency was 20.3 Hz, and the embedding rate was 96.4%, which achieved high-efficiency encapsulation and controlled release of probiotics. This study provides a new idea for high-throughput preparation of L. plantarum microcapsules.
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