食品与发酵工业 >

2023 , Vol. 49 >Issue 5: 74 - 80

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.033899

研究报告

糯红高粱中产蛋白酶内生酵母筛选鉴定及发酵特性研究

  • 胡连清 ,
  • 刘雯雯 ,
  • 刘婉茹 ,
  • 周万海 ,
  • 冯瑞章 ,
  • 魏琴 ,
  • 赵鑫 ,
  • 赵善梅 ,
  • 陈雨薇
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  • 1(宜宾学院 农林与食品工程学部,四川 宜宾,644000)
    2(香料植物资源开发与利用四川省高校重点实验室,四川 宜宾,644000)
    3(简阳市农业技术推广中心,四川 简阳,641400)
第一作者:硕士,助教(周万海副教授为通信作者,E-mail:wanhaizhou@126.com)

收稿日期: 2022-10-10

  修回日期: 2022-11-30

  网络出版日期: 2023-04-06

基金资助

四川省科技厅项目(2018JY0495)

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Screening, identification, and fermentation properties of protease-producing endophytic yeasts from waxy sorghum

  • HU Lianqing ,
  • LIU Wenwen ,
  • LIU Wanru ,
  • ZHOU Wanhai ,
  • FENG Ruizhang ,
  • WEI Qin ,
  • ZHAO Xin ,
  • ZHAO Shanmei ,
  • CHEN Yuwei
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  • 1(Experimental Teaching Center of Agriculture, Forestry and Food Engineering, Yibin University, Yibin 644000, China)
    2(Key Lab of Aromatic Plant Resources Exploitation and Utilization in Sichuan Higher Education, Yibin 644000, China)
    3(Jianyang Agricultural Technology Extension Center, Jianyang 641400, China)

Received date: 2022-10-10

  Revised date: 2022-11-30

  Online published: 2023-04-06

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摘要

以糯红高粱中分离出的内生酵母为研究对象,筛选具有产蛋白酶功能的内生酵母,采用形态学观察和26S rDNA D1/D2 区序列分析对内生酵母进行分类鉴定,并分析其氮源利用能力以及产酶发酵特性。结果发现:从糯红高粱分离得到33株产蛋白酶内生酵母;经26S rDNA D1/D2区序列分析,这些菌株隶属于1门、4目、13属,优势目为银耳目(Tremellales),占57.62%;优势种属为Papiliotrema rajasthanensis,占27.3%。氮源利用能力研究发现,7株内生酵母具有产利用菜籽饼粕氮源的蛋白酶,J3-74菌株产酶能力最强;5株能产利用明胶的蛋白酶,J6菌株产酶能力最优;7株能产利用干酪素的蛋白酶,J3-78菌株的产酶能力最强。对其中2株产蛋白酶菌株的发酵条件优化发现,J6菌株在接种量为1%、pH为9.0、温度为25 ℃、葡萄糖为碳源、牛肉膏为氮源时产蛋白酶的活力最高。而J24菌株在接种量为2%、pH为5.0、温度为25 ℃、葡萄糖为碳源、牛肉膏为氮源时产蛋白酶的活力最高。结果表明,糯红高粱内生酵母种类及其功能具有多样性,可为功能菌株的进一步开发和利用提供支撑。

关键词： 糯红高粱; 蛋白酶; 内生酵母; 发酵特性

本文引用格式

胡连清 , 刘雯雯 , 刘婉茹 , 周万海 , 冯瑞章 , 魏琴 , 赵鑫 , 赵善梅 , 陈雨薇 . 糯红高粱中产蛋白酶内生酵母筛选鉴定及发酵特性研究[J]. 食品与发酵工业, 2023 , 49(5) : 74 -80 . DOI: 10.13995/j.cnki.11-1802/ts.033899

Abstract

The endophytic yeasts isolated from different tissues of waxy sorghum were investigated. Protease-producing yeasts were identified and classified by morphology and 26S rDNA D1/D2 sequencing, and their nitrogen resource utilization and fermentation enzyme production were analyzed. Thirty-three protease-producing endophytic yeasts were isolated from waxy sorghum. Sequencing of 26S rDNA D1/D2 showed that these yeasts belonged to one phylum, four orders, and 13 genera, with the dominant order, Tremellales, accounting for 57.62% of the total. The dominant species was Papiliotrema rajasthanensis which accounted for 27.3% of the total. In terms of nitrogen sources, seven endophytic yeasts produced proteases using rapeseed meal as the nitrogen source, with strain J3-74 showing the highest enzyme production. Five strains produced gelatin-utilizing proteases, with the J6 strain producing the greatest amounts, while seven strains produced casein proteases, with strain J3-78 being the most efficient. The fermentation conditions for protease production were optimized in two strains, and it was found that the J6 strain had the highest protease activity at 1% inoculation, pH 9.0, and 25℃, with glucose as the carbon source and beef paste as the nitrogen source. The protease activity of the J24 strain was highest with 2% inoculation at pH 5.0 and 25 ℃, with glucose as the carbon source and beef paste as the nitrogen source. These findings demonstrate the abundance and diversity of endophytic yeasts in waxy red sorghum and can provide a reference for the further development and utilization of functional strains.

Key words： waxy sorghum; protease; endophytes yeast; fermentation properties

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