该实验在优化膜分离预处理工艺参数的基础上,考察了采用膜分离-树脂吸附的物理方法来调整黄酒酸度指标的工艺过程,并对降酸黄酒的风味品质进行了比较分析。结果表明,在料液温度25 ℃,运行压力1.0 MPa,截留分子质量为200、1 000、2 000 Da的不同膜预处理条件下,D354树脂对高酸黄酒的降酸率分别达到47.0%、53.8%、68.1%。降酸黄酒样品中共检测到32种挥发性风味物质,200、1 000 Da膜预处理组获得的降酸黄酒样品中挥发性风味物质总含量分别达到1 975.89、1 919.80 mg/L,均显著高于未过膜处理组(1 727.04 mg/L)。层次聚类分析表明,1 000 Da和2 000 Da组之间的风味相似度更高,正交偏最小二乘判别分析表明11种化合物对降酸黄酒的风味差异贡献较高。选用1 000 Da膜预处理,结合10%填充比的D354树脂处理50 L高酸黄酒,总酸下降率达57.7%,氨基酸态氮保留率达98.8%,挥发性风味物质的保留率达80.0%。该研究为高酸黄酒的物理降酸提供了新的视角和应用场景。
In this study, the parameters of membrane separation pretreatment were optimized, and the feasibility of reducing the acidity of Huangjiu by the method of membrane separation-resin adsorption was investigated. Then, the flavor of Huangjiu after reducing acidity was evaluated. The results showed that under the Huangjiu temperature of 25 ℃ and the operating pressure of 1.0 MPa, the acid-reducing rate of high-acid Huangjiu after D354 resin adsorption reached 47.0%, 53.8%, and 68.1% respectively, after being pretreated with different membranes with a molecular weight of 200 Da, 1 000 Da, and 2 000 Da. A total of 32 volatile substances were detected in the Huangjiu samples after reducing acidity. The content of volatile substances in the 200 Da (1 975.89 mg/L) and 1 000 Da (1 919.80 mg/L) membrane pretreatment groups were significantly higher than group without membrane pretreatment (1 727.04 mg/L). In addition, the hierarchical cluster analysis showed that higher flavor similarity between 1 000 Da and 2 000 Da groups. Eleven volatile substances with significant differences between different deacidification Huangjiu groups were obtained based on the results of OPLS-DA combined the variable importance in projection. Using 1 000 Da membrane pretreatment combined with 10% filling ratio D354 resin adsorption to reduce the acidity of 50 L high-acid Huangjiu. The results showed that the total deacidification rate reached 57.7% and the retention rates of amino acid nitrogen and volatile components were 98.8% and 80%, respectively. In summary, this study provides a new perspective and application scenario for the physical deacidification of high-acid Huangjiu.
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