综述与专题评论

果蔬花色苷的理化特性、提取技术及功能活性研究进展

  • 张淑淑 ,
  • 吕想 ,
  • 刘伟 ,
  • 张菊华
展开
  • 1(湖南大学 生物学院隆平分院,湖南 长沙,410125)
    2(湖南省农业科学院农产品加工研究所,湖南 长沙,410125)
第一作者:硕士研究生(刘伟副研究员和张菊华研究员为共同通信作者,E-mail:175244822@qq.com;498282528@qq.com)

收稿日期: 2022-10-14

  修回日期: 2022-11-11

  网络出版日期: 2024-02-27

基金资助

湖南省农业科技创新资金项目(2022CX45);湖南省农业科技创新资金项目(2022CX133)

Research progress on physical and chemical characteristics, extraction technology, and functional activity of anthocyanins from fruits and vegetables

  • ZHANG Shushu ,
  • LYU Xiang ,
  • LIU Wei ,
  • ZHANG Juhua
Expand
  • 1(Longping Branch, College of Biology, Hunan University, Changsha 410125, China)
    2(Agricultural Product Processing Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China)

Received date: 2022-10-14

  Revised date: 2022-11-11

  Online published: 2024-02-27

摘要

花色苷是一种水溶性类黄酮化合物,是果蔬等植物中的主要呈色物质。果蔬花色苷具有抗氧化、改善心血管健康、保护视力、预防肥胖、保护神经以及抗肿瘤等生理功能。该文系统介绍了不同果蔬来源花色苷的理化特性、提取方法及其功能活性的研究进展,以期为其在食品和医药领域的深入研究和应用提供参考。

本文引用格式

张淑淑 , 吕想 , 刘伟 , 张菊华 . 果蔬花色苷的理化特性、提取技术及功能活性研究进展[J]. 食品与发酵工业, 2024 , 50(2) : 360 -371 . DOI: 10.13995/j.cnki.11-1802/ts.033971

Abstract

Anthocyanin is a kind of water-soluble flavonoid, which is the main coloring matter in fruits and vegetables. Fruit and vegetable anthocyanins have physiological functions, such as antioxidation, cardiovascular health improvement, eyesight protection, obesity prevention, nerve protection, and anti-tumor. The research progress of physical and chemical characteristics, extraction methods, and functional activities of anthocyanins from different fruits and vegetables were systematically introduced to provide a reference for their further research and application in the field of food and medicine.

参考文献

[1] SILVA S, COSTA E M, CALHAU C, et al.Anthocyanin extraction from plant tissues:A review[J].Critical Reviews in Food Science and Nutrition, 2017, 57(14):3072-3083.
[2] 国石磊.黑果腺肋花楸花色苷分离纯化、结构鉴定及其抗氧化活性研究[D].秦皇岛:河北科技师范学院, 2015.
GUO S L. Purification, structure identification of anthocyanins and its antioxidant activity from Aronia melanocarpa.Qinhuangdao: Hebei Normal University of Science & Technology, 2015.
[3] CHEN L H, HU B, QIN Y H, et al.Advance of the negative regulation of anthocyanin biosynthesis by MYB transcription factors[J].Plant Physiology and Biochemistry, 2019, 136:178-187.
[4] ONGKOWIJOYO P, LUNA-VITAL D A, GONZALE Z DE MEJIA E.Extraction techniques and analysis of anthocyanins from food sources by mass spectrometry:An update[J].Food Chemistry, 2018, 250:113-126.
[5] CASTAÑEDA-OVANDO A, DE LOURDES PACHECO-HERNÁNDEZ M, PÁEZ-HERNÁNDEZ M E, et al.Chemical studies of anthocyanins:A review[J].Food Chemistry, 2009, 113(4):859-871.
[6] 聂泽坤.香樟果花色苷的高效提取及其稳态化性质研究[D].淮安:淮阴工学院, 2021.
NIE Z K. Efficient extraction of anthocyanins from Cinnamomum camphora fruit and its steady-state properties.Huaian: Huaiyin Institute of Technology, 2021.
[7] HOUGHTON A, APPELHAGEN I, MARTIN C.Natural blues:Structure meets function in anthocyanins[J].Plants, 2021, 10(4):726.
[8] HUGHES N M, CONNORS M K, GRACE M H, et al.The same anthocyanins served four different ways:Insights into anthocyanin structure-function relationships from the wintergreen orchid, Tipularia discolor[J].Plant Science, 2021, 303:110793.
[9] 刘晓芬, 李方, 殷学仁, 等.花青苷生物合成转录调控研究进展[J].园艺学报, 2013,40(11):2295-2306.
LIU X F, LI F, YIN X R, et al.Recent advances in the transcriptional regulation of anthocyanin biosynthesis[J].Acta Horticulturae Sinica, 2013,40(11):2295-2306.
[10] CHEN F F, SANG J E, ZHANG Y, et al.Development of a green two-dimensional HPLC-DAD/ESI-MS method for the determination of anthocyanins from Prunus cerasifera var.atropurpurea leaf and improvement of their stability in energy drinks[J].International Journal of Food Science & Technology, 2018, 53(6):1494-1502.
[11] WANG H J, SUN S, ZHOU Z, et al.Rapid analysis of anthocyanin and its structural modifications in fresh tomato fruit[J].Food Chemistry, 2020, 333:127439.
[12] KARAASLAN N M, YAMAN M.Anthocyanin profile of strawberry fruit as affected by extraction conditions[J].International Journal of Food Properties, 2017, 20(sup3):S2313-S2322.
[13] ZHANG Y, CHEN F F, SANG J.Green approach for sample preparation and determination of anthocyanins from Lycium ruthenicum Murr.using a β-cyclodextrin-based extraction method coupled with UPLC-DAD analysis[J].Food Analytical Methods, 2018, 11(8):2141-2148.
[14] XUE H K, TAN J Q, LI Q A, et al.Optimization ultrasound-assisted deep eutectic solvent extraction of anthocyanins from raspberry using response surface methodology coupled with genetic algorithm[J].Foods, 2020, 9(10):1409.
[15] TAN J Q, LI Q A, XUE H K, et al.Ultrasound-assisted enzymatic extraction of anthocyanins from grape skins:Optimization, identification, and antitumor activity[J].Journal of Food Science, 2020, 85(11):3731-3744.
[16] LIU C H, XUE H K, SHEN L Y, et al.Improvement of anthocyanins rate of blueberry powder under variable power of microwave extraction[J].Separation and Purification Technology, 2019, 226:286-298.
[17] SAINZ MARTINEZ A, KORNPOINTNER C, HASELMAIR-GOSCH C, et al.Dynamic streamlined extraction of iridoids, anthocyanins and lipids from haskap berries[J].LWT, 2021, 138:110633.
[18] DEL PILAR GARCIA-MENDOZA M, ESPINOSA-PARDO F A, BASEGGIO A M, et al.Extraction of phenolic compounds and anthocyanins from juçara (Euterpe edulis Mart.) residues using pressurized liquids and supercritical fluids[J].The Journal of Supercritical Fluids, 2017, 119:9-16.
[19] 贾赵东, 马佩勇, 边小峰, 等.植物花青素合成代谢途径及其分子调控[J].西北植物学报, 2014,34(7):1496-1506.
JIA Z D, MA P Y, BIAN X F, et al.Biosynthesis metabolic pathway and molecular regulation of plants anthocyanin[J].Acta Botanica Boreali-Occidentalia Sinica, 2014,34(7):1496-1506.
[20] 李栋, 李莉, 徐艳群, 等.植物中花色苷转运蛋白研究进展[J].食品安全质量检测学报, 2020,11(3):669-674.
LI D, LI L, XU Y Q, et al.Research progress of anthocyanin transporters in plants[J].Journal of Food Safety & Quality, 2020,11(3):669-674.
[21] FEI P, ZENG F S, ZHENG S Y, et al.Acylation of blueberry anthocyanins with maleic acid:Improvement of the stability and its application potential in intelligent color indicator packing materials[J].Dyes and Pigments, 2021, 184:108852.
[22] CORTEZ R, LUNA-VITAL D A, MARGULIS D, et al.Natural pigments:Stabilization methods of anthocyanins for food applications[J].Comprehensive Reviews in Food Science and Food Safety, 2017, 16(1):180-198.
[23] ZHANG Y, BUTELLI E, MARTIN C.Engineering anthocyanin biosynthesis in plants[J].Current Opinion in Plant Biology, 2014, 19:81-90.
[24] CHEN Z Q, WANG C, GAO X D, et al.Interaction characterization of preheated soy protein isolate with cyanidin-3-O-glucoside and their effects on the stability of black soybean seed coat anthocyanins extracts[J].Food Chemistry, 2019, 271:266-273.
[25] 李彬.玫瑰茄花色苷稳定性优化及性质研究[D].天津:天津商业大学, 2022.
LI B.Study on stability optimization and properties of Roselle anthocyanins[D].Tianjin:Tianjin University of Commerce, 2022.
[26] TENA N, ASUERO A G.Up-to-date analysis of the extraction methods for anthocyanins:Principles of the techniques, optimization, technical progress, and industrial application[J].Antioxidants, 2022, 11(2):286.
[27] BARNES J S, NGUYEN H P, SHEN S J, et al.General method for extraction of blueberry anthocyanins and identification using high performance liquid chromatography-electrospray ionization-ion trap-time of flight-mass spectrometry[J].Journal of Chromatography A, 2009, 1216(23):4728-4735.
[28] CHANDRASEKHAR J, MADHUSUDHAN M C, RAGHAVARAO K S M S.Extraction of anthocyanins from red cabbage and purification using adsorption[J].Food and Bioproducts Processing, 2012, 90(4):615-623.
[29] NISTOR M, DIACONEASA Z, FROND A D, et al.Comparative efficiency of different solvents for the anthocyanins extraction from chokeberries and black carrots, to preserve their antioxidant activity[J].Chemical Papers, 2021, 75(2):813-822.
[30] INCE A E, SAHIN S, SUMNU G.Comparison of microwave and ultrasound-assisted extraction techniques for leaching of phenolic compounds from nettle[J].Journal of Food Science and Technology, 2014, 51(10):2776-2782.
[31] WU C Y, WANG H, FAN X H, et al.Waste Euryale ferox salisb.leaves as a potential source of anthocyanins:Extraction optimization, identification and antioxidant activities evaluation[J].Waste and Biomass Valorization, 2020, 11(8):4327-4340.
[32] LIU Y X, ZHAO Y H, ZHUO Y, et al.Ultrasound-assisted extraction of anthocyanins from Malus ‘Royalty’fruits:Optimization, separation, and antitumor activity[J].Molecules, 2022, 27(13):4299.
[33] 宋毓晔, 王渝, 朱千林, 等.植物中花色苷来源及提取方法研究进展[J].食品研究与开发, 2022,43(16):199-208.
SONG Y Y, WANG Y, ZHU Q L, et al.Research progress on sources and extraction methods of plant-based anthocyanin[J].Food Research and Development, 2022,43(16):199-208.
[34] YEDHU KRISHNAN R, RAJAN K S.Microwave assisted extraction of flavonoids from Terminalia bellerica:Study of kinetics and thermodynamics[J].Separation and Purification Technology, 2016, 157:169-178.
[35] 刘雪可, 苏梦飞, 杨宁, 等.不同提取方法对蓝靛果果渣花色苷提取效率的影响[J].食品研究与开发, 2018,39(24):44-48.
LIU X K, SU M F, YANG N, et al.Study on anthocyanins extraction efficiency by different extraction technologies from Lonicera caerulea residue[J].Food Research and Development, 2018,39(24):44-48.
[36] 任济夫, 夏建盟, 周振驰, 等.超临界流体在聚合物工业中的应用研究进展[J].塑料工业, 2022,50(7):12-16;124.
REN J F, XIA J M, ZHOU Z C, et al.Application research processes of supercritical fluid in polymer industry[J].China Plastics Industry, 2022,50(7):12-16;124.
[37] POURMORTAZAVI S M, SAGHAFI Z, EHSANI A, et al.Application of supercritical fluids in cholesterol extraction from foodstuffs:A review[J].Journal of Food Science and Technology, 2018, 55(8):2813-2823.
[38] DEL PILAR SÁNCHEZ-CAMARGO A, PARADA-ALONSO F, IBÁÑEZ E, et al.Recent applications of on-line supercritical fluid extraction coupled to advanced analytical techniques for compounds extraction and identification[J].Journal of Separation Science, 2019, 42(1):243-257.
[39] 田密霞, 李亚东, 胡文忠, 等.响应面分析法优化超临界CO2萃取蓝莓花色苷工艺条件的研究[J].食品工业科技, 2016,37(1):208-212.
TIAN M X, LI Y D, HU W Z, et al.Optimization of supercritical CO2extraction of blueberry anthocyanins using response surface methodology[J].Science and Technology of Food Industry, 2016,37(1):208-212.
[40] PURI M, SHARMA D, BARROW C J.Enzyme-assisted extraction of bioactives from plants[J].Trends in Biotechnology, 2012, 30(1):37-44.
[41] NADAR S S, RAO P, RATHOD V K.Enzyme assisted extraction of biomolecules as an approach to novel extraction technology:A review[J].Food Research International, 2018, 108:309-330.
[42] SWER T L, CHAUHAN K.Stability studies of enzyme aided anthocyanin extracts from Prunus nepalensis L[J].LWT, 2019, 102:181-189.
[43] LI X A, ZHU F Y, ZENG Z W.Effects of different extraction methods on antioxidant properties of blueberry anthocyanins[J].Open Chemistry, 2021, 19(1):138-148.
[44] JOSÉ ALIAÑO GONZÁLEZ M, CARRERA C, BARBERO G F, et al.A comparison study between ultrasound-assisted and enzyme-assisted extraction of anthocyanins from blackcurrant (Ribes nigrum L.)[J].Food Chemistry:X, 2022, 13:100192.
[45] SANTOS L B, SANTOS ASSIS R, BARRETO J A, et al.Deep eutectic solvents in liquid-phase microextraction:Contribution to green chemistry[J].TrAC Trends in Analytical Chemistry, 2022, 146:116478.
[46] WANG Y, CHENG H, HE J R, et al.Enzymes-catalyzed Knoevenagel condensation promoted by ionic liquid and deep eutectic solvent[J].Catalysis Letters, 2022, 152(4):1215-1223.
[47] HIKMAWANTI N P E, RAMADON D, JANTAN I, et al.Natural deep eutectic solvents (NADES):Phytochemical extraction performance enhancer for pharmaceutical and nutraceutical product development[J].Plants, 2021, 10(10):2091.
[48] OZTURK B, PARKINSON C, GONZALEZ-MIQUEL M.Extraction of polyphenolic antioxidants from orange peel waste using deep eutectic solvents[J].Separation and Purification Technology, 2018, 206:1-13.
[49] BI Y H, CHI X W, ZHANG R, et al.Highly efficient extraction of mulberry anthocyanins in deep eutectic solvents:Insights of degradation kinetics and stability evaluation[J].Innovative Food Science & Emerging Technologies, 2020, 66:102512.
[50] ZANNOU O, KOCA I.Greener extraction of anthocyanins and antioxidant activity from blackberry (Rubus spp) using natural deep eutectic solvents[J].LWT, 2022, 158:113184.
[51] FU X Z, WANG D, BELWAL T, et al.Natural deep eutectic solvent enhanced pulse-ultrasonication assisted extraction as a multi-stability protective and efficient green strategy to extract anthocyanin from blueberry pomace[J].LWT, 2021, 144:111220.
[52] 位路路, 林杨, 王月华, 等.黑果腺肋花楸花色苷提取工艺优化及其抗氧化活性和组成鉴定[J].食品科学, 2018,39(12):239-246.
WEI L L, LIN Y, WANG Y H, et al. Optimization of extraction of anthocyanins from berries of Aronia melanocarpa and their antioxidant activity and composition. Food Science, 2018, 39(12):239-246.
[53] NUNES MATTOS G N, PESSANHA DE ARAÚJO SANTIAGO M C P, SAMPAIO DORIA CHAVES A C S D, et al.Anthocyanin extraction from jaboticaba skin (Myrciaria cauliflora Berg.) using conventional and non-conventional methods[J].Foods, 2022, 11(6):885.
[54] DEMIRDÖVEN A, KARABıYıKLı Ş, TOKATLÚ K, et al.Inhibitory effects of red cabbage and sour cherry pomace anthocyanin extracts on food borne pathogens and their antioxidant properties[J].LWT-Food Science and Technology, 2015, 63(1):8-13.
[55] HASSELLUND S S, FLAA A, KJELDSEN S E, et al.Effects of anthocyanins on cardiovascular risk factors and inflammation in pre-hypertensive men:A double-blind randomized placebo-controlled crossover study[J].Journal of Human Hypertension, 2013, 27(2):100-106.
[56] MAURAY A, MILENKOVIC D, BESSON C, et al.Atheroprotective effects of bilberry extracts in apo E-deficient mice[J].Journal of Agricultural and Food Chemistry, 2009, 57(23):11106-11111.
[57] HONG J M, KIM M, KIM B.The effects of anthocyanin-rich bilberry extract on transintestinal cholesterol excretion[J].Foods, 2021, 10(11):2852.
[58] LIU Y X, SONG X, ZHANG D, et al.Blueberry anthocyanins:Protection against ageing and light-induced damage in retinal pigment epithelial cells[J].The British Journal of Nutrition, 2012, 108(1):16-27.
[59] OOE E, KUSE Y, YAKO T, et al.Bilberry extract and anthocyanins suppress unfolded protein response induced by exposure to blue LED light of cells in photoreceptor cell line[J].Molecular Vision, 2018, 24:621-632.
[60] LIU Y X, LIU M, CHEN Q C, et al.Blueberry polyphenols ameliorate visible light and lipid-induced injury of retinal pigment epithelial cells[J].Journal of Agricultural and Food Chemistry, 2018, 66(48):12730-12740.
[61] WU T, TANG Q, YU Z P, et al.Inhibitory effects of sweet cherry anthocyanins on the obesity development in C57BL/6 mice[J].International Journal of Food Sciences and Nutrition, 2014, 65(3):351-359.
[62] XU H Y, LIU M H, LIU H M, et al.Anthocyanins from purple corn ameliorated obesity in high fat diet-induced obese mice through activating hepatic AMPK[J].Journal of Functional Foods, 2021, 84:104582.
[63] 韩雯.樱桃花色苷的降脂作用及其机制研究[D].杭州:浙江大学,2017.
HAN W. Study on lipid-lowering effect and mechanism of cherry anthocyanins.Hangzhou: Zhejiang University, 2017.
[64] PACHECO S M, SOARES M S P, GUTIERRES J M, et al.Anthocyanins as a potential pharmacological agent to manage memory deficit, oxidative stress and alterations in ion pump activity induced by experimental sporadic dementia of Alzheimer′s type[J].The Journal of Nutritional Biochemistry, 2018, 56:193-204.
[65] WEN H C, CUI H, TIAN H H, et al.Isolation of neuroprotective anthocyanins from black chokeberry (Aronia melanocarpa) against amyloid-β-induced cognitive impairment[J].Foods, 2020, 10(1):63.
[66] CHEN L L, JIANG B W, ZHONG C G, et al.Chemoprevention of colorectal cancer by black raspberry anthocyanins involved the modulation of gut microbiota and SFRP2 demethylation[J].Carcinogenesis, 2018, 39(3):471-481.
[67] ZHOU L P, WANG H, YI J J, et al.Anti-tumor properties of anthocyanins from Lonicera caerulea ‘Beilei’fruit on human hepatocellular carcinoma:In vitro and in vivo study[J].Biomedicine & Pharmacotherapy, 2018, 104:520-529.
[68] XU L, TIAN Z Z, CHEN H, et al.Anthocyanins, anthocyanin-rich berries, and cardiovascular risks:Systematic review and meta-analysis of 44 randomized controlled trials and 15 prospective cohort studies[J].Frontiers in Nutrition, 2021, 8: 747884.
[69] LUÍS Â, DOMINGUES F, PEREIRA L.Association between berries intake and cardiovascular diseases risk factors:A systematic review with meta-analysis and trial sequential analysis of randomized controlled trials[J].Food & Function, 2018, 9(2):740-757.
[70] DI PIETRO P, LIZIO R, IZZO C, et al.A novel combination of high-load omega-3 lysine complex (AvailOm©) and anthocyanins exerts beneficial cardiovascular effects[J].Antioxidants, 2022, 11(5):896.
[71] CAMIRE M E.Functional Foods:Herbs, Botanicals and Teas[M].Lancaster:CRC Press, 2000:307-338.
[72] LIU Y X, ZHANG D, HU J M, et al.Visible light-induced lipid peroxidation of unsaturated fatty acids in the retina and the inhibitory effects of blueberry polyphenols[J].Journal of Agricultural and Food Chemistry, 2015, 63(42):9295-9305.
[73] LI X Y, SUN M M, LONG Y.Cyanidin-3-O-glucoside attenuates lipopolysaccharide-induced inflammation in human corneal epithelial cells by inducing Let-7b-5p-mediated HMGA2/PI3K/Akt pathway[J].Inflammation, 2020, 43(3):1088-1096.
[74] LIU J H, HAO W J, HE Z Y, et al.Blueberry and cranberry anthocyanin extracts reduce bodyweight and modulate gut microbiota in C57BL/6 J mice fed with a high-fat diet[J].European Journal of Nutrition, 2021, 60(5):2735-2746.
[75] NUSSBAUM L, HOGEA L M, DANIELA C, et al.Modern treatment approaches in psychoses.Pharmacogenetic, neuroimagistic and clinical implications[J].Farmacia, 2017, 65(1):75-81.
[76] SALEHI B, SHARIFI-RAD J, CAPPELLINI F, et al.The therapeutic potential of anthocyanins:Current approaches based on their molecular mechanism of action[J].Frontiers in Pharmacology, 2020, 11:1300.
[77] CÁSEDAS G, GONZÁLEZ-BURGOS E, SMITH C, et al.Regulation of redox status in neuronal SH-SY5Y cells by blueberry (Vaccinium myrtillus L.) juice, cranberry (Vaccinium macrocarpon A.) juice and cyanidin[J].Food and Chemical Toxicology, 2018, 118:572-580.
[78] LIU J Q, ZHOU H B, SONG L, et al.Anthocyanins:Promising natural products with diverse pharmacological activities[J].Molecules, 2021, 26(13):3807.
文章导航

/