研究报告

负载叶黄素米糠油体乳液的制备及理化稳定性分析

  • 张文冠 ,
  • 郝佳 ,
  • 许朵霞
展开
  • (北京老年营养与健康教育部重点实验室,北京工商大学 食品与健康学院,北京,100048)
第一作者:硕士研究生(许朵霞教授为通信作者,E-mail:xdxbtbu@126.com)

收稿日期: 2022-12-23

  修回日期: 2023-04-24

  网络出版日期: 2024-07-12

基金资助

国家自然科学基金资助项目(32072216);中国食品科学技术学会食品科技基金—雅培食品营养与安全专项科研基金(2021-F04);北京市自然科学基金-北京市教委科技计划重点项目(23JF0005)

Preparation and physicochemical stability analysis of lutein-loaded rice bran oil body emulsion

  • ZHANG Wenguan ,
  • HAO Jia ,
  • XU Duoxia
Expand
  • (Key Laboratory of Geriatric Nutrition and Health (Beijing Technology and Business University), Ministry of Education, School of Food and Health, Beijing Technology and Business University (BTBU), 100048, China)

Received date: 2022-12-23

  Revised date: 2023-04-24

  Online published: 2024-07-12

摘要

叶黄素因其优异的生物活性被广泛关注,但水溶性差、易降解等缺点限制了其在食品加工中的应用。为解决以上问题,该研究以副产物米糠为原料,提取米糠油体作乳化剂,负载叶黄素的稻米油为油相,制备米糠油体叶黄素乳液,对乳液的物理稳定性、包封效果、流变特性、微观结构进行表征,探究乳液组分间相互作用以及乳液的储藏稳定性。研究结果表明,乳液的理化稳定性随着体系中油相浓度的增大而减小,当油相体积分数为10%时,乳液粒径为(481.03±34.39) nm,zeta电位值为(-38.07±0.46) mV;叶黄素与米糠油体主要通过氢键结合;在经过22 d的加速氧化后乳液中叶黄素的保留率接近65%。该研究结果可为开发植物油体递送载体提供理论与技术基础。

本文引用格式

张文冠 , 郝佳 , 许朵霞 . 负载叶黄素米糠油体乳液的制备及理化稳定性分析[J]. 食品与发酵工业, 2024 , 50(11) : 137 -142 . DOI: 10.13995/j.cnki.11-1802/ts.034697

Abstract

Lutein has been widely noticed for its excellent bioactivity, but the disadvantages such as poor water solubility and easy degradation limit its application in food processing.To solve the above problems, in this study, rice bran oil body was extracted as an emulsifier from the by-product rice bran, and rice oil loaded with lutein was used as the oil phase to prepare a lutein-loaded rice bran oil body emulsion.The physical stability, encapsulation effect, rheological properties, and microstructure of the emulsion were characterized, and the interaction between the emulsion components and the storage stability of the emulsion was investigated.Results showed that the physical and chemical stability of the emulsion decreased with the increase of the oil phase concentration in the system, the particle size of the emulsion was (481.03±34.39) nm, and the zeta potential value was (-38.07±0.46) mV when the oil phase concentration was 10%.The lutein and rice bran oil body were mainly bonded by hydrogen bonding, and the retention rate of lutein was nearly 65% after 22 days of accelerated oxidation.The research results can provide a theoretical and technical basis for the development of a plant oil body delivery system.

参考文献

[1] GOMBAČ Z, ČRNIVEC I G O, SKRT M, et al.Stabilisation of lutein and lutein esters with polyoxyethylene sorbitan monooleate, medium-chain triglyceride oil and lecithin[J].Foods, 2021, 10(3):500.
[2] SAINI R K, KEUM Y S.Carotenoid extraction methods:A review of recent developments[J].Food Chemistry, 2018, 240:90-103.
[3] BUSCEMI S, CORLEO D, DI PACE F, et al.The effect of lutein on eye and extra-eye health[J].Nutrients, 2018, 10(9):1321.
[4] 陈艾霖, 洪鹏志, 宋春勇, 等.热处理对负载叶黄素的罗非鱼分离蛋白乳液稳定性和体外消化的影响[J].食品与发酵工业, 2021, 47(16):173-180.
CHEN A L, HONG P Z, SONG C Y, et al.Effect of heat treatment on stability and in vitro digestion of lutein loaded tilapia protein isolate[J].Food and Fermentation Industries, 2021, 47(16):173-180.
[5] WEIGEL F, WEISS J, DECKER E A, et al.Lutein-enriched emulsion-based delivery systems:Influence of emulsifiers and antioxidants on physical and chemical stability[J].Food Chemistry, 2018, 242:395-403.
[6] STEINER B M, MCCLEMENTS D J, DAVIDOV-PARDO G.Encapsulation systems for lutein:A review[J].Trends in Food Science & Technology, 2018, 82:71-81.
[7] 郭静, 胡坦, 潘思轶.食品运载体系包埋叶黄素的研究进展[J].食品科学, 2022, 43(1):313-320.
GUO J, HU T, PAN S Y.Progress in research on food delivery systems for lutein encapsulation[J].Food Science, 2022, 43(1):313-320.
[8] LI J N, GUO R, HU H, et al.Preparation optimisation and storage stability of nanoemulsion-based lutein delivery systems[J].Journal of Microencapsulation, 2018, 35(6):570-583.
[9] XU D X, AIHEMAITI Z, CAO Y P, et al.Physicochemical stability, microrheological properties and microstructure of lutein emulsions stabilized by multilayer membranes consisting of whey protein isolate, flaxseed gum and chitosan[J].Food Chemistry, 2016, 202:156-164.
[10] ABDULLAH, WEISS J, ZHANG H.Recent advances in the composition, extraction and food applications of plant-derived oleosomes[J].Trends in Food Science & Technology, 2020, 106:322-332.
[11] NANTIYAKUL N, FURSE S, FISK I, et al.Phytochemical composition of Oryza sativa (rice) bran oil bodies in crude and purified isolates[J].Journal of the American Oil Chemists’ Society, 2012, 89(10):1867-1872.
[12] SUN Y F, ZHONG M M, WU L C, et al.Loading natural emulsions with nutraceuticals by ultrasonication:Formation and digestion properties of curcumin-loaded soybean oil bodies[J].Food Hydrocolloids, 2022, 124:107292.
[13] HAO J, XU J Z, ZHANG W G, et al.The improvement of the physicochemical properties and bioaccessibility of lutein microparticles by electrostatic complexation[J].Food Hydrocolloids, 2022,125:107381.
[14] LI J L, SHIN G H, LEE I W, et al.Soluble starch formulated nanocomposite increases water solubility and stability of curcumin[J].Food Hydrocolloids, 2016, 56:41-49.
[15] 曾庆晗, 马培华, 邰克东, 等.油相浓度对姜黄素纳米乳液稳定性的影响[J].食品工业科技, 2017, 38(22):17-21.
ZENG Q H, MA P H, TAI K D, et al.Effect of oil phase concentration on the stability of curcumin nanoemulsion[J].Science and Technology of Food Industry, 2017, 38(22):17-21.
[16] 王成祥, 刘辉, 段胜林, 等.应用快速稳定性分析方法研究增稠剂对燕麦饮料稳定性的影响[J].食品与发酵工业, 2018, 44(3):253-259.
WANG C X, LIU H, DUAN S L, et al.Research on the effect of thickening on the stability of oat beverage using rapid stability analysis instrument[J].Food and Fermentation Industries, 2018, 44(3):253-259.
[17] NAKAUMA M, FUNAMI T, NODA S, et al.Comparison of sugar beet pectin, soybean soluble polysaccharide, and gum Arabic as food emulsifiers.1.Effect of concentration, pH, and salts on the emulsifying properties[J].Food Hydrocolloids, 2008, 22(7):1254-1267.
[18] 瓦文强, 秦娟娟, 杨敏, 等.酪蛋白胶束乳液凝胶性质及其在大黄素负载中的应用[J].食品与发酵工业, 2023, 49(1):132-139.
WA W Q, QIN J J, YANG M, et al.Properties of emulsion gel based on casein micelles and its application in emodin loading[J].Food and Fermentation Industries, 2023, 49(1):132-139.
[19] LÓPEZ-MONTERRUBIO D I, LOBATO-CALLEROS C, VERNON-CARTER E J, et al.Influence of β-carotene concentration on the physicochemical properties, degradation and antioxidant activity of nanoemulsions stabilized by whey protein hydrolyzate-pectin soluble complexes[J].LWT, 2021, 143:111148.
[20] CHEVALIER R C, GOMES A, CUNHA R L.Role of aqueous phase composition and hydrophilic emulsifier type on the stability of W/O/W emulsions[J].Food Research International, 2022, 156:111123.
[21] YU X X, ZHAO Y J, SUN M, et al.Effects of egg yolk lecithin/milk fat globule membrane material ratio on the structure and stability of oil-in-water emulsions[J].LWT, 2022,168:113891.
[22] 刘杰, 邓利玲, 曾云军, 等.糖基化改性对明胶乳化性和抗氧化活性的影响[J].食品与发酵工业, 2023, 49(19):175-182.
LIU J, DENG L L, ZENG Y J, et al.Effects of glycosylation modification on emulsifying property and antioxidant activity of gelatin[J].Food and Fermentation Industries, 2023, 49(19):175-182.
[23] LIU G, WANG Q, HU Z Z, et al.Maillard-reacted whey protein isolates and epigallocatechin gallate complex enhance the thermal stability of the Pickering emulsion delivery of curcumin[J].Journal of Agricultural and Food Chemistry, 2019, 67(18):5212-5220.
[24] 李文婧. 醇溶蛋白/原花青素/果胶复合纳米颗粒稳定Pickering乳液的制备及其负载姜黄素的研究[D].泰安:山东农业大学, 2022.
LI W J.Pickering emulsions stabilized by zein-proanthocyanidins-pectin composites and its application for curcumin loading[D].Tai’an:Shandong Agricultural University, 2022.
[25] GONG T, CHEN B, HU C Y, et al.Resveratrol inhibits lipid and protein co-oxidation in sodium caseinate-walnut oil emulsions by reinforcing oil-water interface[J].Food Research International, 2022, 158:111541.
文章导航

/