将不同结构的人工乳脂肪球膜作为乳化剂和营养强化剂加入到婴配乳中,并以富含天然乳脂肪球膜(milk fat globule membrane, MFGM)成分的MFGM-10为对照,并通过模拟婴幼儿体外消化模型对比了其在胃和小肠消化阶段的粒径分布、电位变化和游离脂肪酸释放量等关键指标,以探究不同结构人工MFGM对婴配乳理化性质和脂肪消化特性的影响。结果表明,添加单层人工MFGM的婴配乳具有较小的平均粒径和较大的ζ-电位绝对值,同时具有较高的乳化活性指数和乳化稳定性指数,说明单层人工MFGM在改善婴配乳理化性质和提高乳液稳定性方面效果最佳。在消化结束后,各组释放游离脂肪酸总量的顺序为对照组1 560 μmol/mL＞双层组1 415 μmol/mL＞单层组1 361 μmol/mL＞空白组1 048 μmol/mL,说明添加人工MFGM能够达到与天然MFGM相似的脂肪消化效果。该研究为人工MFGM代替天然MFGM在婴配乳中的应用提供一定理论依据。
The artificial milk fat globule membrane with different structures was added into infant formula milk as an emulsifier and nutrient enhancer, and the MFGM-10 rich in natural MFGM components was used as a control.Through the simulation of an infant in vitro digestion model, the key indicators such as particle size distribution, electric potential change, and free fatty acid release in the digestive stage of the stomach and small intestine were compared to explore the effects of different structures of artificial MFGM on the physicochemical properties and fat digestion characteristics of infant formula milk.Results showed that the infant formula added with monolayer artificial MFGM had a smaller average particle size and larger absolute value of ζ-potential, high emulsifying activity index and emulsifying stability index, and the smallest range of particle size change during digestion indicated that single-layer artificial MFGM had the best effect in improving the physical and chemical properties of infant formula milk and improving the stability of lotion.The order of the total amount of released free fatty acids among different groups after digestion completion is as follows:control group 1 560 μmol/mL >bilayer group 1 415 μmol/mL >monolayer group 1 361 μmol/mL >blank group 1 048 μmol/mL, indicating that adding artificial MFGM could achieve similar fat digestion effect to natural MFGM.This study provides a theoretical basis for the application of artificial MFGM instead of natural MFGM in infant formula.
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