食品与发酵工业 >

2024 , Vol. 50 >Issue 17: 47 - 57

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039682

研究报告

稀土盐与泡沫分离强化乳酸乳球菌生产乳链菌肽

  • 艾慕凡 ,
  • 刘伟 ,
  • 杨春燕 ,
  • 殷昊 ,
  • 卢珂
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  • (河北工业大学 化工学院,天津,300401)
第一作者：硕士研究生(刘伟副教授为通信作者,E-mail:liuw16018@hebut.edu.cn)

收稿日期: 2024-04-24

  修回日期: 2024-06-03

  网络出版日期: 2024-10-10

基金资助

国家自然科学基金青年项目(22108059)

收起

Promotion of Nisin production by Lactococcus lactis through adding rare earth salt and performing foam fractionation

  • AI Mufan ,
  • LIU Wei ,
  • YANG Chunyan ,
  • YIN Hao ,
  • LU Ke
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  • (School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300401, China)

Received date: 2024-04-24

  Revised date: 2024-06-03

  Online published: 2024-10-10

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摘要

乳链菌肽(Nisin)是一种重要的食品防腐剂,但其工业应用却受限于较低的生产效率。该研究拟采用稀土盐和泡沫分离强化乳酸乳球菌生产Nisin。利用摇瓶试验研究了8种稀土盐对菌体生长和Nisin生物合成的影响。以Nisin的回收率和发酵液的总Nisin效价为指标,利用单因素试验和响应面优化试验获得了发酵与泡沫分离耦合操作的最佳工艺条件。结果表明,所选的8种稀土盐中,添加氯化铥(TmCl3)至发酵培养基能够大幅增加菌体生物量和Nisin产量。在发酵9 h添加1×10-4 mol/L的TmCl3,发酵液的Nisin效价达到(5 643.23±156.57) IU/mL。在发酵与泡沫分离初始耦合时间18.50 h,气体分布器孔径300 μm和气体体积流率135 mL/min条件下,Nisin的回收率和发酵液的总Nisin效价分别为(89.43±2.35)%和(6 159.00±89.35) IU/mL。综上,适量添加稀土盐和执行泡沫分离,能够在促进菌体生长的同时缓解产物抑制,实现Nisin的高效生产。该研究提出的外源辅因子刺激和产物原位分离方法对于强化Nisin的工业生产具有重要意义。

关键词： 乳链菌肽; 稀土元素; 泡沫分离; 响应面优化试验

本文引用格式

艾慕凡 , 刘伟 , 杨春燕 , 殷昊 , 卢珂 . 稀土盐与泡沫分离强化乳酸乳球菌生产乳链菌肽[J]. 食品与发酵工业, 2024 , 50(17) : 47 -57 . DOI: 10.13995/j.cnki.11-1802/ts.039682

Abstract

Nisin is an important food preservative but its industrial utilization is limited by the low production efficiency.This work attempted to promote Nisin production by Lactococcus lactis through adding rare earth salt and performing foam fractionation.Flask-shaking tests had been conducted to investigate the effects of eight rare earth salts on strain growth and Nisin biosynthesis.With the recovery percentage of Nisin and the total Nisin titer of fermentation broth as the indexes, the optimal conditions of fermentation-foam fractionation coupling operation were obtained by using single factor test and response surface optimization test.Results indicated that among the selected eight rare earth elements, cell biomass and Nisin production could be dramatically enhanced by adding TmCl3 into fermentation medium.When adding time of TmCl3 was 9 h, the Nisin titer of fermentation broth was as high as (5 643.23±156.57) IU/mL.Under the conditions of initial coupling time of fermentation and foam fractionation 18.50 h, pore diameter of gas distributor 300 μm and volumetric air flow rate 135 mL/min, the recovery percentage of Nisin and total Nisin titer of fermentation broth were (89.43±2.35)% and (6 159.00±89.35) IU/mL, respectively.To sum up, it was conducive to achieving the efficient production of Nisin by moderately adding rare earth salt and performing foam fractionation, which could promote strain growth and mitigate product inhibition, simultaneously.In this work, the strategies of exogenous cofactor stimulation and in-situ product separation were of great significance to promoting the industrial production of Nisin.

Key words： Nisin; rare earth element; foam fractionation; response surface optimization test

参考文献

[1] 张阳玲, 吴昊, 乔建军, 等.乳酸链球菌肽的应用及研究进展[J].食品与发酵工业, 2020, 46(7):289-295.
ZHANG Y L, WU H, QIAO J J, et al.Application and research progress of Nisin[J].Food and Fermentation Industries, 2020, 46(7):289-295.
[2] GORAIN C, SINGH A, BHATTACHARYYA S, et al.Mucosal delivery of live Lactococcus lactis expressing functionally active JlpA antigen induces potent local immune response and prevent enteric colonization of Campylobacter jejuni in chickens[J].Vaccine, 2020, 38(7):1630-1642.
[3] NI Z J, ZHANG X Y, LIU F, et al.Effect of co-overexpression of nisin key genes on nisin production improvement in Lactococcus lactis LS01[J].Probiotics and Antimicrobial Proteins, 2017, 9(2):204-212.
[4] GIRGIN ERSOY Z, KAYIHAN C, TUNCA S.Higher nisin yield is reached with glutathione and pyruvate compared with heme in Lactococcus lactis N8[J].Brazilian Journal of Microbiology, 2020, 51(3):1247-1257.
[5] NAKAGAWA T, MITSUI R, TANI A, et al.A catalytic role of XoxF1 as La3+-dependent methanol dehydrogenase in Methylobacterium extorquens strain AM1[J].PLoS One, 2012, 7(11):e50480.
[6] 王硕, 时唐恩, 赵春光, 等.稀土元素对L-酪氨酸发酵的影响研究[J].中国调味品, 2023, 48(2):1-6;24.
WANG S, SHI T E, ZHAO C, et al.Study on effect of rare earth elements on fermentation of L-tyrosine[J].China Condiment, 2023, 48(2):1-6;24.
[7] 熊华仪, 陈曦, 刘月锋, 等.补料策略优化促进乳酸乳球菌HB03发酵合成Nisin[J].食品科学, 2023, 44(22):218-224.
XIONG H Y, CHEN X, LIU Y F, et al.Optimization of fed-batch culture strategy for improved synthesis of nisin by Lactococcus lactis HB03[J].Food Science, 2023, 44(22):218-224.
[8] LEE S H, KIM S, KIM J Y, et al.Enhanced butanol fermentation using metabolically engineered Clostridium acetobutylicum with ex situ recovery of butanol[J].Bioresource Technology, 2016, 218:909-917.
[9] 鲁吉珂, 黎业娟, 吴霄玥, 等.有机溶剂沉淀法提取乳酸链球菌素的效果[J].食品科学, 2012, 33(10):84-86.
LU J K, LI Y J, WU X Y, et al.Separation of nisin from fermentation broth concentrate by organic solvent precipitation[J].Food Science, 2012, 33(10):84-86.
[10] TAFRESHI S Y H, MIRDAMADI S, KHATAMI S.Comparison of different nisin separation and concentration methods:Industrial and cost-effective perspectives[J].Probiotics and Antimicrobial Proteins, 2020, 12(3):1226-1234.
[11] ORABY A, WEICKARDT I, ZIBEK S.Foam fractionation methods in aerobic fermentation processes[J].Biotechnology and Bioengineering, 2022, 119(7):1697-1711.
[12] HOU D D, YU W T, ZHANG D M, et al.Culture of yeast cells immobilized by alginate-chitosan microcapsules in aqueous-organic solvent biphasic system[J].Journal of Oceanology and Limnology, 2019, 37(3):863-870.
[13] CHENG Q Y, TAO J, LI Y, et al.Production of nisin and lactic acid from the starch of sweet potato by simultaneous saccharification and fermentation with two stage pH adjustment[J].3 Biotech, 2021, 11(7):320.
[14] ZHAO G, LIU J M, ZHAO J, et al.Efficient production of nisin A from low-value dairy side streams using a nonengineered dairy Lactococcus lactis strain with low lactate dehydrogenase activity[J].Journal of Agricultural and Food Chemistry, 2021, 69(9):2826-2835.
[15] KAZAK E S, KALITINA E G, KHARITONOVA N A, et al.Biosorption of rare-earth elements and yttrium by heterotrophic bacteria in an aqueous environment[J].Moscow University Geology Bulletin, 2018, 73(3):287-294.
[16] LYU K, WANG X, WANG L, et al.Rare-earth element yttrium enhances the tolerance of curly-leaf pondweed (Potamogeton crispus) to acute nickel toxicity[J].Environmental Pollution, 2019, 248:114-120.
[17] WEHRMANN M, BERTHELOT C, BILLARD P, et al.Rare earth element (REE)-dependent growth of Pseudomonas putida KT2440 relies on the ABC-transporter PedA1A2BC and is influenced by iron availability[J].Frontiers in Microbiology, 2019, 10:2494.
[18] 霍光华, 张冬艳, 张通.稀土离子(Nd3+)对金黄色葡萄球菌细胞壁结构的影响[J].分子细胞生物学报, 2007, 40(6):437-442.
HUO G H, ZHANG D Y, ZHANG T.Some effects of the neodymium ion on the cell wall structure of staphyloccocus aruea[J].Journal of Molecular Cell Biology, 2007, 40(6):437-442.
[19] HEIDARI S, HAMEDI J, OLAD G, et al.Structural and functional evaluation of recombinant histidine phosphokinase NisK and response regulator NisR:In silico and experimental approach[J].World Journal of Microbiology & Biotechnology, 2019, 35(11):169.
[20] YANG C Y, SU Z W, LI Z F, et al.Harvest of nisin from fermentation broth using foam separation with the assistance of ultrasonic treatment:Foam property evaluation and antimicrobial activity retention[J].Separation and Purification Technology, 2023, 311:123253.
[21] 胡滨, 朱海兰, 吴兆亮.气体分布器孔径对泡沫分离过程影响的研究[J].高校化学工程学报, 2014, 28(2):246-251.
HU B, ZHU H L, WU Z L.The effect of the pore size of gas distributor on foam separation process[J].Journal of Chemical Engineering of Chinese Universities, 2014, 28(2):246-251.
[22] KRIEGEL A T, DUCKER W A.Removal of bacteria from solids by bubbles:Effect of solid wettability, interaction geometry, and liquid-vapor interface velocity[J].Langmuir, 2019, 35(39):12817-12830.
[23] WU H, LIU J G, MIAO S, et al.Contribution of YthA, a PspC family transcriptional regulator of Lactococcus lactis F44 acid tolerance and nisin yield:A transcriptomic approach[J].Applied and Environmental Microbiology, 2018, 84(6):e02483-e02417.
[24] KÖRDIKANLIOĞLU B, ŞIMŞEK Ö, SARIS P E J.Nisin production of Lactococcus lactis N8 with hemin-stimulated cell respiration in fed-batch fermentation system[J].Biotechnology Progress, 2015, 31(3):678-685.
[25] QIAO W J, QIAO Y, GAO G, et al.A novel co-cultivation strategy to generate low-crystallinity bacterial cellulose and increase nisin yields[J].International Journal of Biological Macromolecules, 2022, 202:388-396.
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