研究报告

明胶与多糖复合膜制备及对冷藏虹鳟脂质氧化的影响

  • 李晨辉 ,
  • 黄丽 ,
  • 白淑艳 ,
  • 葛凯博 ,
  • 覃东立 ,
  • 陈中祥 ,
  • 王鹏 ,
  • 高磊
展开
  • 1(中国水产科学研究院黑龙江水产研究所,黑龙江 哈尔滨,150070)
    2(农业农村部渔业环境及水产品质量监督检验测试中心(哈尔滨),黑龙江 哈尔滨,150070)
    3(农业农村部淡水水产生物技术与遗传育种重点实验室,黑龙江 哈尔滨,150070)
第一作者:硕士,助理研究员(高磊助理研究员和王鹏研究员为共同通信作者,E-mail:gaolei@hrfri.ac.cn;wangpeng@hrfri.ac.cn)

收稿日期: 2023-10-17

  修回日期: 2023-12-11

  网络出版日期: 2024-10-29

基金资助

中央级公益性科研院所基本科研业务费专项(HSY202112Q);中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金资助项目(2023TD60)

Preparation of composite films of gelatin and polysaccharides and effect on lipid oxidation of rainbow trout during cold storage

  • LI Chenhui ,
  • HUANG Li ,
  • BAI Shuyan ,
  • GE Kaibo ,
  • QIN Dongli ,
  • CHEN Zhongxiang ,
  • WANG Peng ,
  • GAO Lei
Expand
  • 1(Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China)
    2(Supervision, Inspection and Testing Center for Fishery Environment and Aquatic Products(Harbin), Ministry of Agriculture and Rural Affairs, Harbin 150070, China)
    3(Key Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Harbin 150070, China)

Received date: 2023-10-17

  Revised date: 2023-12-11

  Online published: 2024-10-29

摘要

寻找天然大分子化合物制备可食用可降解食品包装材料已成为研究热点。该研究以明胶为基质材料制备可食性保鲜膜,并分别复合壳聚糖、果胶及海藻酸钠3种多糖,研究多糖的添加对明胶膜物理性质、微观结构及对冷藏虹鳟脂质氧化情况的影响。膜的微观结构表明多糖与明胶之间形成了均一稳定的结构,红外光谱显示添加多糖影响了O—H键的弯曲和伸缩振动,从而影响了氢键的形成。果胶和海藻酸钠的添加将膜的水蒸气透过性提高,同时壳聚糖将明胶膜的透光率最多降低30%。虹鳟冷藏实验结果表明,复合膜处理的虹鳟将菌落总数可接受限值从9 d延长至12 d左右,硫代巴比妥酸反应产物值也有类似结果,脂肪酸构成表明复合膜的处理延缓了不饱和脂肪酸的氧化。综上,明胶经多糖复合后形成的活性包装材料具有良好的应用前景。

本文引用格式

李晨辉 , 黄丽 , 白淑艳 , 葛凯博 , 覃东立 , 陈中祥 , 王鹏 , 高磊 . 明胶与多糖复合膜制备及对冷藏虹鳟脂质氧化的影响[J]. 食品与发酵工业, 2024 , 50(19) : 209 -215 . DOI: 10.13995/j.cnki.11-1802/ts.037685

Abstract

The search for natural macromolecular compounds to prepare edible and degradable food packaging materials has become a research hotspot.Edible preservation film was prepared by gelatin as matrix material, and chitosan, pectin, and sodium alginate were compounded separately.The research aimed to study the effects of the addition of polysaccharides in gelatin film on the physical properties, microstructure, and lipid oxidation of rainbow trout during cold storage.The microstructure of the films showed that a homogeneous and stable structure was formed between the polysaccharide and gelatin.The infrared spectrum showed that the addition of polysaccharide affected the bending and stretching vibration of the O—H bond, thus affecting the formation of the hydrogen bond.The addition of pectin and sodium alginate improved the water vapor permeability of the film, while chitosan reduced the light transmittance of the gelatin film by up to 30%.The acceptable limit of total viable counts of rainbow trout treated with composite film was extended from 9 days to about 12 days, and the thiobarbituric acid reactive substance(TBARs)value had similar results.The fatty acid composition showed that the oxidation of unsaturated fatty acid was delayed by the treatment of composite film.In conclusion, the active packaging material formed by the compound of gelatin and polysaccharide has a good application prospect.

参考文献

[1] 刘磊. 罗非鱼鳞胶原蛋白与多糖复合材料的制备及表征[D].南宁:广西大学, 2022.
LIU L.Preparation and characterization of tilapia scale collagen and polysaccharide composites[D].Nanning:Guangxi University, 2022.
[2] 王娇. 明胶基可食性复合膜的制备及性能研究[D].杨凌:西北农林科技大学, 2022.
WANG J.Preparation and properties of gelatin-based edible composite film[D].Yangling:Northwest A & F University, 2022.
[3] 陈洪彬, 李书亮, 蒋璇靓, 等.鱼皮明胶-壳聚糖复合涂膜对‘黄金'西番莲的保鲜效果[J].食品与发酵工业, 2022, 48(18):134-141.
CHEN H B, LI S L, JIANG X J, et al.Effects of fish skin gelatin-chitosan composite coating film on the preservation of passion (Passiflora caerulea L.) fruit[J].Food and Fermentation Industries, 2022, 48(18):134-141.
[4] YE X Q, LIU R R, QI X Y, et al.Preparation of bioactive gelatin film using semi-refined pectin reclaimed from blueberry juice pomace:Creating an oxidation and light barrier for food packaging[J].Food Hydrocolloids, 2022, 129:107673.
[5] 巫丽君, 黄邵丰, 周涛, 等.鱼明胶改性技术及其产品在食品包装中应用研究进展[J].食品与发酵工业, 2022, 48(21):341-348.
WU L J, HUANG S F, ZHOU T, et al.Research process on fish gelatin modification technologies and its application in food packaging[J].Food and Fermentation Industries, 2022, 48(21):341-348.
[6] LEE M, RÜEGG N, YILDIRIM S.Evaluation of the antimicrobial activity of sodium alginate films integrated with cinnamon essential oil and citric acid on sliced cooked ham[J].Packaging Technology and Science, 2023, 36(8):647-656.
[7] ZHANG N, HAN J, CHEN F L, et al.Chitosan/gum Arabic complexes to stabilize Pickering emulsions:Relationship between the preparation, structure and oil-water interfacial activity[J].Food Hydrocolloids, 2022, 129:107532.
[8] WANG P K, GAO Y F, WANG D Q, et al.Amidated pectin with amino acids:Preparation, characterization and potential application in Hydrocolloids[J].Food Hydrocolloids, 2022, 129:107662.
[9] CENGIZ N, GUCLU G, KELEBEK H, et al.GC-MS-olfactometric characterization of key odorants in rainbow trout by the application of aroma extract dilution analysis:Understanding locational and seasonal effects[J].Food Chemistry, 2023, 407:135137.
[10] 杜宁宁, 黄晓丽, 郝其睿, 等.八种鲑科鱼类肌肉营养成分分析与营养价值评价[J].水产学杂志, 2022, 35(4):9-18;37.
DU N N, HUANG X L, HAO Q R, et al.Analysis of nutritional components and evaluation of nutritive quality in muscle of eight members in Salmonidae[J].Chinese Journal of Fisheries, 2022, 35(4):9-18;37.
[11] SURÓWKA K, RZEPKA M, ÖZOĞUL F, et al.Nucleotide degradation, biogenic amine level and microbial contamination as quality indicators of cold-stored rainbow trout (Oncorhynchus mykiss) gravad[J].Food Chemistry, 2021, 346:128904.
[12] 李晨辉, 于晓倩, 董汝月, 等.明胶/壳聚糖/迷迭香复合膜的制备与表征[J].食品工业科技, 2019, 40(10):57-61;67.
LI C H, YU X Q, DONG R Y, et al.Preparation and characterization of composite films incorporated with chitosan, gelatin and rosemary[J].Science and Technology of Food Industry, 2019, 40(10):57-61;67.
[13] 陈柯君, 杨周昊, 余虹达, 等.基于黑苹果果皮花青素的虾鲜度指示膜的制备及应用[J].食品科学, 2023,44(21):274-281.
CHEN K J, YANG Z H, YU H D, et al.Preparation and application of shrimp freshness indicator film based on black apple peel anthocyanin[J].Food Science, 2023,44(21):274-281.
[14] LONG J Y, ZHANG W Y, ZHAO M Z, et al.The reduce of water vapor permeability of polysaccharide-based films in food packaging:A comprehensive review[J].Carbohydrate Polymers, 2023, 321:121267.
[15] 刘俊霞, 赵萍, 金晶, 等.基于气相色谱-离子迁移谱结合化学计量学分析大鲵肉冷藏期间挥发性成分[J].食品与发酵工业, 2022, 48(22):269-278.
LIU J X, ZHAO P, JIN J, et al.Analysis of volatile components in giant salamander meat during cold storage based on gas chromatography-ion mobility spectrometer and chemometrics[J].Food and Fermentation Industries, 2022, 48(22):269-278.
[16] SINGH S, BHAT H F, KUMAR S, et al.Storage stability of chocolate can be enhanced using locust protein-based film incorporated with E.purpurea flower extract-based nanoparticles[J].Ultrasonics Sonochemistry, 2023, 100:106594.
[17] 刘冬敏, 王建辉, 刘永乐, 等.草鱼肌肉脂肪酸组成及其在冷藏中的含量变化[J].食品科学, 2013, 34(2):194-198.
LIU D M, WANG J H, LIU Y L, et al.Changes in fatty acid composition during cold storage of fresh grass carp muscle[J].Food Science, 2013, 34(2):194-198.
[18] LI X, SHA X M, YANG H S, et al.Ultrasonic treatment regulates the properties of gelatin emulsion to obtain high-quality gelatin film[J].Food Chemistry:X, 2023, 18:100673.
[19] 秦春歌. 果胶基膜的制备及其结构性能研究[D].泰安:山东农业大学,2022.
QIN C G.Preparation and structural properties of pectin-based films[D].Tai'an:Shandong Agricultural University, 2022.
[20] MARIA T M C, DE CARVALHO R A, SOBRAL P J A, et al.The effect of the degree of hydrolysis of the PVA and the plasticizer concentration on the color, opacity, and thermal and mechanical properties of films based on PVA and gelatin blends[J].Journal of Food Engineering, 2008, 87(2):191-199.
[21] 李夜平, 徐云, 程晓农, 等.反相细乳液交联法制备裂隙结构壳聚糖纳米粒[J].江苏大学学报(自然科学版), 2014, 35(1):120-124.
LI Y P, XU Y, CHENG X N, et al.Preparation of chitosan nanoparticles with fissuaring structure by inverse miniemulsion cross-linking method[J].Journal of Jiangsu University (Natural Science Edition), 2014, 35(1):120-124.
[22] 戴卿印, 周鑫, 黄茜, 等.大豆分离蛋白-壳聚糖可食用性抗菌膜的制备与性能评价[J].粮食与油脂, 2022, 35(6):89-95.
DAI Q Y, ZHOU X, HUANG X, et al.Preparation and performance evaluation of soybean protein isolate-chitosan edible antibacterial film[J].Cereals & Oils, 2022, 35(6):89-95.
[23] ŁUPINA K, KOWALCZYK D, DROZŁOWSKA E.Polysaccharide/gelatin blend films as carriers of ascorbyl palmitate - A comparative study[J].Food Chemistry, 2020, 333:127465.
[24] SHUANG F F, ZONG C M, WANG C C, et al.Chlorogenic acid and cellulose nanocrystal-assisted crosslinking preparation of a silk-based film to extend the shelf life of strawberries[J].LWT, 2022, 172:114218.
[25] 沈秋霞, 李明元, 朱克永, 等.3种保鲜剂及其复配对虹鳟鱼片冷藏品质的影响[J].食品与机械, 2019, 35(11):145-151.
SHEN Q X, LI M Y, ZHU K Y, et al.Effects of three kinds of preservatives and their complexation on the refrigerated quality of rainbow trout[J].Food & Machinery, 2019, 35(11):145-151.
[26] NABATI S, AMINZARE M, ROOHINEJAD S, et al.Electrospun polycaprolactone nanofiber containing Ganoderma lucidum extract to improve chemical and microbial stability of rainbow trout fillets during storage at 4 ℃[J].Food Control, 2023, 150:109777.
[27] SALDANHA T, BENASSI M T, BRAGAGNOLO N.Fatty acid contents evolution and cholesterol oxides formation in Brazilian sardines (Sardinella brasiliensis) as a result of frozen storage followed by grilling[J].LWT - Food Science and Technology, 2008, 41(7):1301-1309.
[28] 王馨云, 谢晶.不同冷藏条件下金枪鱼的水分迁移与脂肪酸变化的相关性[J].食品科学, 2020, 41(5):200-206.
WANG X Y, XIE J.Correlation between changes in water mobility and fatty acid profile of tuna during cold storage[J].Food Science, 2020, 41(5):200-206.
[29] 陈桂平, 赵晨, 卢君, 等.茶多酚对草鱼冷藏过程脂肪酸的影响[J].中国粮油学报, 2013, 28(10):44-48;54.
CHEN G P, ZHAO C, LU J, et al.Influence of tea polyphenols in lipid changes of grass carp during cold storage[J].Journal of the Chinese Cereals and Oils Association, 2013, 28(10):44-48;54.
文章导航

/