For the further development and application of natural composite fresh-keeping coating, chitosan-silymarin composite fresh-keeping coating was prepared with chitosan as film-forming substrate, silymarin as main bacteriostatic agent and 0%, 2%, 4%, 6% and 8% based on the mass of chitosan.The effects of different silymarin addition on the properties of composite fresh-keeping coating were investigated by measuring DPPH radical scavenging rate, zeta potential and rheological properties.At the same time, the pH, juice loss rate and total volatile basic nitrogen (total volatile basic nitrogen, TVB-N), thiobarbituric acid reactive substances (TBARS), the total number of colonies and sensory evaluation to explore the effects of different silymarin addition on pork cold storage.The experimental results showed that with the increase of silymarin content, the antioxidant activity of each group of fresh-keeping coatings increased significantly, and the zeta potential was greater than 30 mV, which showed typical non-Newtonian shear thinning fluid behavior and had good stability.Among them, the preservative coating with 8% silymarin had the best effect on the preservation of pork in cold storage.After 12 days of cold storage, compared with the control group, the preservative coating with 2%, 4%, 6%, and 8% silymarin reduced the pH of pork by 15.92%, 19.39%, 21.56%, and 23.30%, respectively, and the juice loss rate decreased by 19.58%、22.76%, 31.33%, and 39.16%, TBARS decreased by 25.97%, 39.75%, 58.35%, and 64.9%, and TVB-N decreased by 23.50%, 30.56%, 47.71%, and 52.35%.Therefore, chitosan-silymarin composite coating can delay the deterioration of chilled pork quality, and can be used as an excellent edible coating in food preservation.
[1] 陈智慧, 陈健晴, 刘香香, 等.多糖类、蛋白类及其复合涂膜在采后浆果保鲜中应用研究进展[J].保鲜与加工, 2022, 22(3):97-103.
CHEN Z H, CHEN J Q, LIU X X, et al.Research progress of application of polysaccharides, proteins and their composite coatings in preservation of postharvest berries[J].Storage and Process, 2022, 22(3):97-103.
[2] 王安杏, 曹川, 张庆, 等.壳聚糖复合膜在果蔬保鲜中的应用[J].食品安全质量检测学报, 2023, 14(5):164-172.
WANG A X, CAO C, ZHANG Q, et al.Application of chitosan composite film in fruits and vegetables preservation[J].Journal of Food Safety & Quality, 2023, 14(5):164-172.
[3] 戴照琪, 罗元鑫, 许俊齐, 等.茶多酚-壳聚糖复合涂膜对秀珍菇保鲜效果的影响[J].食品研究与开发, 2022, 43(22):42-47.
DAI Z Q, LUO Y X, XU J Q, et al.Effect of chitosan-tea polyphenol composite coating on the preservation of Pleurotus geesteranus[J].Food Research and Development, 2022, 43(22):42-47.
[4] 陈洪彬, 李书亮, 蒋璇靓, 等.鱼皮明胶-壳聚糖复合涂膜对‘黄金’西番莲的保鲜效果[J].食品与发酵工业, 2022, 48(18):134-141.
CHEN H B, LI S L, JIANG X J, et al.Effects of fish skin gelatin-chitosan composite coating film on the preservation of passion (Passiflora caerulea L.) fruit[J].Food and Fermentation Industries, 2022, 48(18):134-141.
[5] 李佳艺, 陈赛, 刘永乐, 等.壳聚糖复合保鲜对草鱼肌肉品质变化的影响[J].食品科学, 2021, 42(7):220-225.
LI J Y, CHEN S, LIU Y L, et al.Effect of chitosan coating incorporated with food preservatives on the muscle quality of grass carp during cold storage[J].Food Science, 2021, 42(7):220-225.
[6] ESMAEIL N, ANARAKI S B, GHARAGOZLOO M, et al.Silymarin impacts on immune system as an immunomodulator:One key for many locks[J].International Immunopharmacology, 2017, 50:194-201.
[7] SHAH M, ULLAH M A, DROUET S, et al.Interactive effects of light and melatonin on biosynthesis of silymarin and anti-inflammatory potential in callus cultures of Silybum marianum (L.) gaertn[J].Molecules, 2019, 24(7):1207.
[8] 杨思进, 白雪, 蒲清荣, 等.一种调节肝脏机能的饮料及其制备方法和用途:中国,CN110973411A[P].2020-04-10.
YANG S J, Bai X, PU Q R, et al.A beverage for regulating liver function, its preparation method and use:China,CN110973411A[P].2020-04-10.
[9] 石崧. 一种用于保护肝脏的含有水飞蓟素的新型食品配方:中国,CN113367340A[P].2021-09-10.
SHI J.A new food formula containing silymarin for protecting liver:China,CN113367340A[P].2021-09-10.
[10] 马琳,马兴群,韩强, 等.一种具有能够修复肝脏的甜菜碱功能饮品:中国,CN111296837A[P].2020-06-19.
MA L, MA X Q, HAN Q, et al.A betaine functional drink that can repair the liver:China,CN111296837A[P].2020-06-19.
[11] 刘剑桥,孟莹,朱艳华.水飞蓟素缓释制剂的研究进展[J].中国药房,2017,28(28):4011-4014.
LIU J Q, MENG Y, ZHU Y H.Research progress of silymarin sustained-release preparations[J].China Pharmacy, 2017,28(28):4011-4014.
[12] 杨延音, 杨治国, 胡世国, 等.水飞蓟素在皮肤科的功效及其在化妆品中的应用进展[J].日用化学工业, 2019, 49(4):259-263.
YANG Y Y, YANG Z G, HU S G, et al.The efficacy of silymarin in dermatology and its application in cosmetics[J].China Surfactant Detergent & Cosmetics, 2019, 49(4):259-263.
[13] DE OLIVEIRA D R, TINTINO S R, BRAGA M F B M, et al.In vitro antimicrobial and modulatory activity of the natural products silymarin and silibinin[J].BioMed Research International, 2015, 2015:292797.
[14] EVREN E, YURTCU E.In vitro effects on biofilm viability and antibacterial and antiadherent activities of silymarin[J].Folia Microbiologica, 2015, 60(4):351-356.
[15] WANG H S, MA Y L, LIU L, et al.Incorporation of clove essential oil nanoemulsion in chitosan coating to control Burkholderia gladioli and improve postharvest quality of fresh Tremella fuciformis[J].LWT, 2022, 170:114059.
[16] DAI Q Y, HUANG X, JIA R J, et al.Development of antibacterial film based on alginate fiber, and peanut red skin extract for food packaging[J].Journal of Food Engineering, 2022, 330:111106.
[17] SUN Y N, ZHANG M, BHANDARI B, et al.Nanoemulsion-based edible coatings loaded with fennel essential oil/cinnamaldehyde:Characterization, antimicrobial property and advantages in pork meat patties application[J].Food Control, 2021, 127:108151.
[18] MI H B, ZHAO B, WANG C, et al.Effect of chitosan coating enriched with 6-gingerol on red drum fillets quality and shelf life during refrigerated storage[J].Journal of Aquatic Food Product Technology, 2017, 26(9):1032-1041.
[19] 许萍, 黄敏, 廖涛, 等.大蒜素复配保鲜剂对大口黑鲈保鲜效果的影响[J].食品科学技术学报, 2021, 39(4):148-155.
XU P, HUANG M, LIAO T, et al.Effects of allicin compound preservative on preservation of Micropterus salmoides[J].Journal of Food Science and Technology, 2021, 39(4):148-155.
[20] 杨欧, 张晓湘, 徐小涵, 等.抗氧化型壳聚糖/大豆蛋白复合食用膜的制备与应用[J].食品工业科技, 2024,45(6):210-218.
YANG O, ZHANG X X, XU X H, et al.Preparation and application of antioxidant chitosan/soybean protein composite edible film[J].Food Industry Science and Technology, 2024,45(6):210-218.
[21] HUANG M Y, WANG H H, XU X L, et al.Effects of nanoemulsion-based edible coatings with composite mixture of rosemary extract and ε-poly-L-lysine on the shelf life of ready-to-eat carbonado chicken[J].Food Hydrocolloids, 2020, 102:105576.
[22] DU H J, HU Q H, YANG W J, et al.Development, physiochemical characterization and forming mechanism of Flammulina velutipes polysaccharide-based edible films[J].Carbohydrate Polymers, 2016, 152:214-221.
[23] 严碧云, 李西, 黄爱妮, 等.壳聚糖蒜姜复合膜在冷却猪肉保鲜中的应用[J].中国食品添加剂,2021, 32(12):72-81.
YAN B Y, LI X, HUANG A N, et al.Application of chitosan ginger garlic composite film in chilled pork preservation[J].China Food Additives, 2021, 32(12):72-81.
[24] 范小宁,陈敬敬,赵瑞娜,等.桑叶提取物-壳聚糖可食性涂膜对静宁烧鸡保鲜效果的研究[J].食品与发酵工业,2024,50(11):201-208.
FAN X N, CHEN J J, ZHAO R N, et al.Study on the fresh-keeping effect of mulberry leaf extract-chitosan edible coating on Jingning roast chicken[J].Food and Fermentation Industries, 2024,50(11):201-208.
[25] 郗泽文,成策,彭盛峰, 等.柠檬精油乳液可食用涂膜液对冷藏卤鸭脖的保鲜效果[J].食品科学,2020,41(1):237-243.
XI Z W, CHENG C, PENG S F, et al.Effect of lemon essential oil emulsion edible coating on quality preservation of refrigerated pot-stewed duck neck[J].Food Science, 2020,41(1):237-243.