食品与发酵工业 >

2024 , Vol. 50 >Issue 23: 276 - 283

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038292

研究报告

机械伤胁迫对香蕉采后膜脂代谢的影响

  • 陈瑜娴 ,
  • 黄春霞 ,
  • 李丽 ,
  • 孙健 ,
  • 易萍 ,
  • 李静 ,
  • 黄方 ,
  • 黄敏 ,
  • 甘婷 ,
  • 隆宇涵
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  • 1(桂林理工大学 化学与生物工程学院,广西 桂林,541006)
    2(广西壮族自治区农业科学院农产品加工研究所,广西 南宁,530007)
    3(广西果蔬贮藏与加工新技术重点实验室,广西 南宁,530007)
    4(广西壮族自治区农业科学院,广西 南宁,530007)
第一作者:硕士研究生(孙健研究员和易萍助理研究员为共同通信作者,E-mail:jiansun@gxaas.net;pingyi@gxaas.net)

收稿日期: 2023-12-18

  修回日期: 2024-02-01

  网络出版日期: 2024-12-27

基金资助

国家自然科学基金项目(32160732,32272784);国家香蕉产业技术体系项目(CARS-31);广西科技基地和人才专项项目(桂科AD23023014);科技先锋队‘强农富民’‘六个一’专项行动(桂农科盟202315)

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Effects of mechanical damage stress on membrane lipid metabolism of postharvest banana

  • CHEN Yuxian ,
  • HUANG Chunxia ,
  • LI Li ,
  • SUN Jian ,
  • YI Ping ,
  • LI Jing ,
  • HUANG Fang ,
  • HUANG Min ,
  • GAN Ting ,
  • LONG Yuhan
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  • 1(College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541006, China)
    2(Agro-Food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China)
    3(Guangxi Key Laboratory of Fruits and Vegetables Storage-Processing Technology, Nanning 530007, China)
    4(Guangxi Academy of Agricultural Sciences, Nanning 530007, China)

Received date: 2023-12-18

  Revised date: 2024-02-01

  Online published: 2024-12-27

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摘要

该研究从细胞膜降解角度研究机械损伤对香蕉果实的影响。为保持香蕉采后品质提供理论依据,该研究以“桂蕉6号”香蕉为试验材料,对香蕉样品进行机械伤处理,并在25 ℃、相对湿度90%~95%条件下贮藏15 d,测定贮藏过程中香蕉果皮细胞膜渗透率、细胞膜降解关键酶活性、细胞膜磷脂含量及磷脂酶D(phospholipase D,PLD)基因表达量等相关指标的变化。结果表明,机械伤处理导致采后香蕉贮藏期间果皮细胞膜渗透率上升,香蕉果皮的磷脂酶C(phospholipase C,PLC)、PLD、脂氧合酶(lipoxygenase,LOX)、甘油二酯激酶(diacylglycerol kinase,DGK)、脂磷酸磷酸酶(lipid phosphate phosphatase,LPP)的酶活性上升,且显著高于对照组(P＜0.05);机械伤胁迫使果皮膜磷脂含量发生变化,磷脂酰乙醇胺(phosphatidyl ethanolamine,PE)含量下降,磷脂酸(phosphatidic acid,PA)、磷脂酰胆碱(phosphatidylcholine,PC)、甘油二酯(diacylglycerol,DAG)含量累积;机械伤诱导果皮MaPLD1及MaPLD4基因表达量增加,且显著高于对照组(P＜0.05),同时抑制了MaPLD3基因的表达。综合分析可知,机械损伤胁迫通过加速膜脂代谢,导致香蕉果皮细胞膜结构和功能破坏,是香蕉果实采后品质下降的主要原因之一。该研究旨在探讨机械损伤对香蕉果实采后品质的影响及其机理,为提高香蕉果实采后储存品质提供理论依据。

关键词： 机械损伤; 香蕉; 膜脂代谢; 酶活; 磷脂酶

本文引用格式

陈瑜娴 , 黄春霞 , 李丽 , 孙健 , 易萍 , 李静 , 黄方 , 黄敏 , 甘婷 , 隆宇涵 . 机械伤胁迫对香蕉采后膜脂代谢的影响[J]. 食品与发酵工业, 2024 , 50(23) : 276 -283 . DOI: 10.13995/j.cnki.11-1802/ts.038292

Abstract

The mechanism of mechanical damage in banana fruit after harvest was studied from the perspective of cell membrane degradation, which provided a theoretical foundation for preserving the postharvest quality of bananas.In this study, banana fruit (Guijiao No.6) was subjected to mechanical damage and then stored at a temperature of 25 ℃ and a relative humidity of 90%-95% for 15 days.Changes in cell membrane permeability, activity of crucial enzymes for cell membrane degradation, content of phospholipids in the cell membrane and gene expression of PLD during the storage of banana peels were determined.Results showed that mechanical damage treatment resulted in an increase in the permeability of peel cell membrane during postharvest banana storage.The activity levels of PLC, PLD, LOX, DGK, and LPP of banana peel during storage were significantly higher than those in the control group (P<0.05).Additionally, the mechanical damage treatment resulted in a decrease in the content of PE and an accumulation of PA, PC, and DAG.The relative expression level of MaPLD1 and MaPLD4 genes increased, which were significantly higher than that of the control group (P<0.05), whereas the relative expression of the MaPLD3 gene in banana peel was inhibited by mechanical damage treatment.Comprehensive analysis revealed that mechanical damage stress is a primary cause of postharvest quality decline in banana fruit.This stress accelerated membrane lipid metabolism, leading to the destruction of the structure and function of banana pericarp cell membranes.The objective of this study is to examine the impact of mechanical damage on the postharvest quality of banana fruit and its underlying mechanism.This will provide a theoretical foundation for enhancing the postharvest storage quality of banana fruit.

Key words： mechanical damage; banana; membrane lipid metabolism; enzyme activity; phospholipase

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