25 March 2018, Volume 44 Issue 3
    

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  • LIU Chun-li et al
    Food and Fermentation Industries. 2018, 44(3): 1. https://doi.org/10.13995/j.cnki.11-1802/ts.015189
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    With the detection of ethanol by ethanol sensor, the effects of ethanol on cell growth and astaxanthin production by Phaffia rhodozyma JMU-MVP14 were investigated in shake flask. The results indicated that 1~2 g/L ethanol was conducive to astaxanthin accumulation in initial medium with 10 g/L or 20 g/L glucose, while high concentration of alcohol inhibited the cell growth and astaxanthin production of P. rhodozyma. When ethanol concentration was maintained at 2 g/L in initial medium with 10 g/L glucose, astaxanthin production was 50.1 mg/L, which increased by 20.3% compared with the control group. The yield of astaxanthin in a cell was 13.1 mg/g and the utilization rate of glucose was over 77.2%. The results demonstrated that the constant ethanol control strategy was conductive to astaxanthin accumulation in yeast cells. The maximum yield of astaxanthin was 55.3 mg/L under the optimized condition of 10 g/L initial glucose concentration, 2 g/L constant ethanol concentration and 5 g/L glucose supplemented per 24 h during the fermentation. And the yield of astaxanthin in a cell was 19.7 mg/g, which increased by 39.7% compared with the control group (14.1 mg/g).
  • LAN Na-na et al
    Food and Fermentation Industries. 2018, 44(3): 8. https://doi.org/10.13995/j.cnki.11-1802/ts.015358
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    Komagataella phaffii is a famous host for heterologous protein expression. However, methanol is needed as inducer and lead to toxic and explosive danger when it is used at large amount of industrial scale. Therefore, non-methanol inducer is urgent needed and thus become a hot topic in this research field. In this research, a recombinant Komagataella phaffii expressing xylanase was used to explore non-methanol inducer. The conditions of methanol induction were optimized to obtain 3 403.8 U/mL xylanase, and 97.5 U/mL OD600 specific xylanase. 40.9% intensity of AOX1 promoter under methanol induction was obtained under 0.5% ammounim formate induction. And 2 032.0 U/mL xylanase was obtained at the condition of 0.5% ammonium formate and 0.5% sorbitol addition per 24 h, which was 1.54 fold of AOX1 promoter intensity at the condition of methanol induction. Therefore, ammonium formate combined with sorbitol was an efficient inducer for heterologous protein expression of Komagataella phaffii.
  • XU Le et al
    Food and Fermentation Industries. 2018, 44(3): 15. https://doi.org/10.13995/j.cnki.11-1802/ts.015964
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    Glucose tolerance factor (GTF) can effectively regulate sugar metabolism, improve the affinity of insulin to receptor, and improve fat metabolism. Up to now, its chromic value in GTF is unclear, which limited its application in reducing sugar health food. In this paper, extraction of GTF with ammonia and ethanol method from high chromium yeast was optimized. And then glucose tolerance factor (GTF) with high purity was obtained by two stages of gel chromatography. HPLC-ICP-MS was used to analyze chromium valence of GTF. The high chromium yeast was treated with 0.1 mol/L of ammonium for 4 h and then 30% of ethanol for 20 min. The total chromium in this extraction was 133 μg. Compared with ammonia extraction alone, the ratio of chromium to protein was increased by 6 times in this way. After extraction, glucose tolerance factor was purified by tow chromatography consecutively. The purified GTF was analyzed by HPLC-ICP-MS. Its chromatography showed that there was only one peak of chromium in this sample. It appeared in range from 31 s to 90 s, which was in agreement with standard of trivalent chromium. Therefore, it could be concluded that chromium in GTF exist in the state of trivalent. This will provide basis for research and development of high chromium yeast for dietary supplement.
  • DONG Wen-hua et al
    Food and Fermentation Industries. 2018, 44(3): 22. https://doi.org/10.13995/j.cnki.11-1802/ts.015589
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    A strain which could produce a high concentration of jasmonic acid was screened from more than 100 molds in nature and preserved in the laboratory. It was named as Lasiodiplodia iranensis DWH-2. Optimized fermentation medium composition contained sucrose 50 g/L, NaNO3 4.5 g/L, KH2PO4 2 g/L, KCl 0.4 g/L, MgSO4·7H2O 6 g/L, FeSO4·7H2O 0.9 g/L, corn pulp 1.5g/L, trace element 10 ml/L, soybean oil 10g/L, and its initial pH value was 5.5. The culture media volume and culture temperature was optimized to 100 mL/500 mL and 28 ℃, respectively. Under these conditions, the yield of jasmonic acid could reach 1.543 g/L after 7 days.
  • LIU Zun et al
    Food and Fermentation Industries. 2018, 44(3): 30. https://doi.org/10.13995/j.cnki.11-1802/ts.014230
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    To evaluate antifungal activity of these flaxseed cyclolinopeptides, FCLPs, two strains, Paecilomyces varioti bainier(AS 3.776)and Aspergillus flavus(CICC 2476),were selected as test strains and inoculated on potato dextrose agar (PDA) with FCLPs of serial concentrations added before or after autoclave. IC50 of FCLPs mixtures on spores formation of P. varioti bainier were 0.53 mg/mL (autoclaved) and 0.34 mg/mL (not autoclaved), and IC50 on A. flavuswere were 0.31 mg/mL (autoclaved) and 0.24 mg/mL (not autoclaved), respectively. Most of antifungal activity of FCLPs was retained after autoclave. Residual antifungal activity against Paecilomyces varioti bainier were found as much as 76.8% (radial growth) and 76.7% (spores formation) of original activity, and those gainst A. flavus were as much as 61.6% (radial growth) and 41.7% ( spores formation), respectively. Results of spores germination test indicated that the average inhibition rate against P. varioti bainier (17.8%) was higher than that against A. flavus(7.8%). After fungi culture, FCLPs were extracted from PDA and analyzed by high performance liquid chromatography (HPLC). Results indicated that the content of FCLPs in the media was decreased due to autoclave and thus led to the decrease of antifungal activity. In this study, FCLPs were found to have antifungal activity. And the activity partially remained after autoclave. It is indicated that FCLPs had the advantages over current antimicrobial peptides applied in food processing involved with heat treatment。
  • MA Ya-ru et al
    Food and Fermentation Industries. 2018, 44(3): 35. https://doi.org/10.13995/j.cnki.11-1802/ts.015983
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    The foaming and emulsifying properties of a range of sucrose stearates were studied by measuring the content of monoester, diester, polyester as well as their hydrophile-lipophile balance (HLB) values. The relationship between its composition and functional properties has been revealed. Furthermore, using sucrose and stearic acid ethyl ester as the substrates, mono-sucrose stearate was enzymatic synthesized in the presence of phase transfer catalyst (myristyltrimethylammonium bromide). The physico-chemical properties of the associate product were investigated. Results showed that the HLB values were decreased with the decreasing of the content of sucrose stearate monoester, which showing a significant correlation. The lower HLB values and lower content of sucrose stearate monoester usually resulted in decreased foaming ability, foam stability and emulsion stability of the series of sucrose stearate. However, the emulsifying ability was observed to be increased prior to decreasing in this process. The structure of mono-sucrose stearate synthesized herein has been confirmed by 1H-NMR, 13C-NMR and ESI-MS with a HLB value of 14.90. Its foaming ability, foam stability and emulsifying ability as well as emulsion stability were also evaluated. The present work provided a theoretical basis for the application and green synthesis of sucrose stearates.
  • LI Chan-juan et al
    Food and Fermentation Industries. 2018, 44(3): 41. https://doi.org/10.13995/j.cnki.11-1802/ts.015979
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    Overlapping extension PCR was used to replace the amino acid residue His 297 (H) by Phe (F) near the activity center of XynA from Geobacillus stearothermophilus. XynA and XynAH297F were separately expressed in Escherichia coli BL21 (DE3) and were both purified and characterized. The optimum pH for XynAH297F was 5.5, which was similar to that for XynA. After treatment in pH4-10 buffers over 20hs, the residual activities of XynA and XynAH297F were both above 90%, which indicated that they had good pH stability. The optimum temperature for XynAH297F (75 ℃) was increased compared with XynA (70 ℃). In addition, the XynA and XynAH297F respectively remained about 10% and 30% residual activities after incubated at 80 ℃ for 20 min. It proved that the thermal stability of XynAH297F was improved at certain degree. Compared with XynA, XynAH297F displayed increases in Km, Vmax and Kcat. The results showed that the affinity of enzyme to substrate was decreased, but the catalytic rate was increased.
  • DING Lin et al
    Food and Fermentation Industries. 2018, 44(3): 47. https://doi.org/10.13995/j.cnki.11-1802/ts.016233
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    Taking apparent enzyme activity of immobilized papain as the index, the effects of ultrasound frequency and power on the kinetics of immobilized papain chemical reaction were investigated. It was found that the decomposing reaction of casein by the immobilized papain is consistent with the first-order reaction rate formula. Ultrasonic treatment can significantly reduce the apparent activation energy. The ultrasonic power was 0.05 W/cm2, and the ultrasonic frequency was 28, 40, 50 and 135 kHz, the apparent activation energy decreased gradually, and the apparent activation energy of 38.23±0.53 kJ/mol at 135 kHz 0.25 W/cm2 was the lowest. When the ultrasonic frequency was 135 kHz and the ultrasonic power was increased from 0.05 W/cm2 to 0.45 W/cm2, the apparent activation energy of 32.81±0.09 kJ/mol was the lowest under the ultrasonic condition of 135 kHz 0.25 W/cm2, which was decreased by 52.48% compared with that before ultrasonic treatment.
  • MA Wen-rui et al
    Food and Fermentation Industries. 2018, 44(3): 52. https://doi.org/10.13995/j.cnki.11-1802/ts.015744
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    Using Vitis vinifera as raw material, the effect of main components in polymerase chain reaction (PCR) system on the simple sequence repeats (SSR) amplification of vitis vinifera was studied to optimize dosage of each factor. Total DNA of Grape was extracted by improved hexadecyl trimethyl ammonium bromide (CTAB) extraction method. The influences of the concentrations of main components in SSR-PCR system such as Mg2+, dNTPs, Taq DNA polymerase, primers and the template the vitis vinifera DNA were analyzed through the single factor experiments. Based on orthogonal optimization design, the optimal 25μL reaction system for SSR-PCR of vitis vinifera DNA was established as Mg2+ of 2.5 mmol/L, dNTPs of 0.15 mmol/L, Taq DNA polymerase of 1.5 U, primers of 0.5 μmol/L, and DNA template of 40 ng. Based on visual analysis and variance analysis of optimization experiments, the influences of main components in this PCR system were Mg2+>Taq DNA polymerase>dNTPs>primers>template DNA. 4 kinds of vitis vinifera DNA were amplified using this SSR-PCR amplification system and products were detected using non denaturing polyacrylamide gel electrophoresis. There are clear and stable amplification bands in gels, which indicated that this system could be used to study the SSR mark of vitis vinifera.
  • SHA Xiao-mei et al
    Food and Fermentation Industries. 2018, 44(3): 58. https://doi.org/10.13995/j.cnki.11-1802/ts.014933
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    The glycated grass carp (Ctenopharyngodon idella) scale gelatin was tested in vitro simulated gastrointestinal digestion to study the antioxidant properties of enzymatic hydrolysis peptides. In the process of gastric digestion, the scavenging capacity of DPPH· and ·OH first increased and then decreased, while the Fe2+ chelating ability first decreased and then increased. In the process of gastrointestinal digestion, DPPH· scavenging ability increased to a stable value, while ·OH scavenging ability of glycation products were less than non-glycated fish scale gelatin. In addition, Fe2+ chelating ability showed inconspicuous change. When the glycation reaction time extended, the degree of glycation increased gradually, and glycation reaction reduces the digestive properties of fish scale gelatin in the gastrointestinal tract. In addition, the glycation reaction can reduce the digestive activity of fish gelatin in gastrointestinal and affect the anti-oxidant activities of its digestions.
  • REN Guang-xu et al
    Food and Fermentation Industries. 2018, 44(3): 65. https://doi.org/10.13995/j.cnki.11-1802/ts.014787
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    To investigate the effects of dual protein on exercise fatigue, 18 healthy and SPF grade wistar male rats were randomly divided into 3 groups: soy protein(n=6), whey protein(n=6), and dual protein groups(n=6). We used weight loaded force swimming test to evaluate anti-fatigue capacity. Tail venous blood was collected to detect the activity of creatine kinase and lactate dehydrogenase at certain time period. After six weeks of feeding, mean exhaustive time of the rats fed with dual protein can reach to135.8 s which was significantly higher than the whey protein group (88 s) and soybean protein group (99.8 s). Lactate dehydrogenase activity of dual protein group reached to pick value in 30 min and went back to the same level as others in 60 min. Thus, supplement of dual protein after exercise can help recover anti-fatigue quickly and improve the endurance capacity of wistar rats.
  • LIN Long et al
    Food and Fermentation Industries. 2018, 44(3): 70. https://doi.org/10.13995/j.cnki.11-1802/ts.015257
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    Engraulis japonicus cooking liquid is a by-product from fish processing. It contains various components and rich of nutrients. In order to purify the cooking liquid, chitosan was used as a safe flocculant to separate and recycle proteins from cooking liquid and help membrane concentrating efficiency in the later process. The effect of Chitosan on the flocculation of water soluble protein in cooking liquor was studied under different pH conditions. The analysis of solubility curve, infrared spectrum, molecular weight distribution, heavy metal and amino acid content showed that: the flocculation effect was the best at pH=3, and the removal rate of water soluble protein was 36.23 %. However, considering of the simplicity and economics in the industry, pH=5.5 was selected to move about about 30% water soluble protein. The flocculation mechanism of chitosan was different in different pH environment. In acidic environment, it was mainly depended on charge neutralization and bridging effects to form crosslinking structure between protein and chitosan; under alkaline conditions, the adsorption was dominant in the flocculating process. The pH affected the flocculation of macromolecular water-soluble proteins with chitosan, while the water-soluble proteins whose molecular weight was less than 19kDa remained unaffected. In addition, chitosan flocculation would reduce the content of heavy metals, but it does not affect the change of amino acid content.
  • WANG Zhi-hui et al
    Food and Fermentation Industries. 2018, 44(3): 77. https://doi.org/10.13995/j.cnki.11-1802/ts.015181
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    Consumer acceptance on yellow tea was studied according to chemical analysis on main taste compounds and taste attribute. The results showed that there was significant difference among 33 kinds of main taste compounds in yellow tea. The order of the significant contribution was: sweetness>mellow>flesh flavor>bitterness>astringency>sourness. Among these factors, the fresh flavor has the biggest difference. Consumer acceptance was significantly positive related to sweetness, mellow, flesh flavor, and negatively related to bitterness, astringency. Twenty- one kinds of main taste compounds and taste attributes have a great correlation; some synergistic effects were showed in main taste in yellow tea infusion. Cluster analysis can be used to collect the yellow tea samples with the same taste characteristics and consumer acceptance, and can be used as a new method to pair tea taste with consumer's acceptance of yellow tea. Moreover, a preliminarily partial least squares(PLS) regression model was constructed based on main taste compounds to predict the consumer acceptance of yellow tea.
  • ZHAO Tian-tian et al
    Food and Fermentation Industries. 2018, 44(3): 84. https://doi.org/10.13995/j.cnki.11-1802/ts.014986
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    This study was designed to analysis the effects of different degree of polymerization (DP) inulin on wheat dough rheological properties and bread quality. Inulin of 3 different DP [DP-L (DP=4.3), DP-M (DP=11.37), DP-H (DP=19.02)] were added to wheat flour at a certain amount, and wheat flour without inulin was set as the control. The quality of blended wheat, stretching, dynamic rheological properties, and bread quality were measured. Results showed that three kinds of DP inulin could obviously improve the quality and tensile property of wheat flour. Dough water absorption increased significantly with DP increased. Three DP inulin had also extended the dough formation time compared with the control group. Dough stability with DP-H increased and dough weakening reduced, while DP-M and DP-L group had no significant differences compared to the control group. DP inulin can increase the extent, tension ratio, curve area and the maximum tensile resistance of dough. From dynamic rheological properties results, G' and G'' value of DP-H group were the highest, followed by DP-M, DP-L and control group. It suggested that three DP inulin all could increase the elasticity of the dough. According to temperature scanning, three DP inulin can increase dough gelatinization temperature, elasticity and viscosity. DP-H and DP-M inulin decreased bread fermentation volume and bread specific volume which suggested that those two DP insulins had no improvement on bread quality. DP-L group could increase bread fermentation volume and bread specific volume indicating that DP-L inulin could delay the ageing of the bread and improve bread quality.
  • HOU Jing et al
    Food and Fermentation Industries. 2018, 44(3): 91. https://doi.org/10.13995/j.cnki.11-1802/ts.015999
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    Haloarchaeal strains isolated from Chaidamin Salt Lake, Inner Mongolia were screened for high protease activity. Their enzyme properties were studied. The results showed that CDM10 and CDM12 displayed the highest protease activity, and represented novel taxa. The optimal protease activity of CDM10 occurred at 70-75 ℃, pH 8.0-9.0, and 1.5 mol/L NaCl. The CDM12 protease exhibited maximal activity at 60-75 ℃, pH 7.0-9.0, and 1.5-2.5 mol/L NaCl. The CDM10 and CDM12 proteases were highly stable over wide ranges of temperatures and NaCl concentrations. The inhibitor experiment suggested that the CDM10 and CDM12 proteases belonged to serine protease family, and the cysteine residues and metal ions were important for the enzyme activities.
  • HUANG Guo-chang et al
    Food and Fermentation Industries. 2018, 44(3): 97. https://doi.org/10.13995/j.cnki.11-1802/ts.016064
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    Phenyllactic acid is a new biological preservative with broad antibacterial spectrum. In order to obtain high yield lactic acid bacteria, 2 lactic acid bacteria strains with high phenyllactic acid yield were isolated from 50 samples in different environment with the modified methods of dissolving calcium ring and bacteriostatic circle, which were named respectively as BLPC002 and SCPC008, and the strains were identified. The results showed that the yield of phenyllactic acid in shake flask fermentation with phenylalanine as substrate was 358 mg/L and 333 mg/L, respectively. The yields of phenyllactic acid were evidently improved compared with less than 100 mg/L before modification. It indicated that the modified method was more efficient and accurate. The strains were identified as Lactobacillus plantarum by morphological analysis and 16S rDNA sequencing.
  • XIONG Yan-yan et al
    Food and Fermentation Industries. 2018, 44(3): 101. https://doi.org/10.13995/j.cnki.11-1802/ts.015962
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    In order to obtain a chitosanase high-yield strain, separated and purified Aspergillus cervinus ZJOU-AC1 was used as raw material to carry out cold source plasma mutagenesis and screening, then the single factor and L9(34) orthogonal tests were used to optimize the fermentation conditions of the mutant strain. Through the experiment, the optimal mutation time was set as 90s. The stable mutant strain of Aspergillus cervinus, ZJOU-AC2, was obtained and its chitosanase activity was increased by 50.7% compared with the original strain. The optimum fermentation conditions were as follows: the fermentation temperature was 30℃, the initial pH was 6.0, the fermentation time was 90h and the inoculation amount was 3%. At this time, the chitosanase activity in the fermentation broth was improved by 15.07%.
  • ZHANG Xue-qin et al
    Food and Fermentation Industries. 2018, 44(3): 107. https://doi.org/10.13995/j.cnki.11-1802/ts.015936
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    Response surface methodology and fuzzy mathematics evaluation were applied to optimize the fermentation process of Antarctic krill. Taking sensory score and amino nitrogen content as indexes, the single factor experiment was carried out on the proportion of bacteria, fermentation temperature, fermentation time, and inoculation quantity of compound starter. Based on the results of single factor experiments, the experiment was designed for 3 factors and 3 levels according to the Box-Behnken center combination method, and the response surface analysis was carried out. The results showed that the optimum conditions for the fermentation of Antarctic krill were the ratio of Lactobacillus plantarum to Saccharomyces rouxii to Staphylococcus xylosus of 1∶2∶3, fermentation temperature of 31 ℃, fermentation time of 22h, inoculum size of 4%. Under these conditions, the total score of sensory evaluation of fermented Antarctic krill broth was 4.39. The fermented broth is nutritious and full of flavor with unique and harmonious taste.
  • HAN Xue et al
    Food and Fermentation Industries. 2018, 44(3): 121. https://doi.org/10.13995/j.cnki.11-1802/ts.016154
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    Jujube pulb was fermented by five kinds of lactic acid bacteria (Streptococcus thermophiles, Lactobacillusacidophilus, Lactobacillus casei, Lactobacillus delbrueckii subsp. Bulgaricus, Lactobacillus plantarum subsp. Plantarum), and the content, composition and antioxidant activity of free phenolic acid were determined. The results showed that five kinds of lactic acid bacteria all could increase the total free phenol content, DPPH radical scavenging ability and FRAP antioxidant capacity to different degree. And Lactobacillus plantarum subsp. Plantarum had advantage over the other lactic acid bacteria in improvement of the content of free phenolic acid and antioxidant ability. After 24 h, the total free phenol content, DPPH radical scavenging ability and FRAP antioxidant capacity were increased by 41.7%, 139.3% and 53.8%, respectively. The contents of salicylic acid, 4-hydroxybenzoic acid, vanillic acid, protocatechuic acid and coumaric acid were increased by 22.8%-121.1%. Therefore, the fermented jujube pulb with high antioxidant activity could be obtained after fermentation of Lactobacillus plantarum subsp. Plantarum for 24 h, and thus might be developed as functional food.
  • FAN Hong-mei et al
    Food and Fermentation Industries. 2018, 44(3): 128. https://doi.org/10.13995/j.cnki.11-1802/ts.015584
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    The β-glucan was hydrolyzed by β-glucanase. The single-factor and the response surface (RSM) test were used to optimize the enzymolysis process with the yield of water-soluble yeast β-glucan as an index. The structure and thermal stability of water-soluble yeast β-glucan were examined. The results showed that the optimum conditions were substrate concentration of 1.14 g/dL, enzyme activity of 0.16 U/mL, temperature of 44 ℃, pH value of 4.2. The highest yield of water-soluble yeast β-glucan was 39.89%. The results of infrared spectroscopy showed that the glycosidic bond of water-soluble yeast β-glucan and the original yeast β-glucanwere β-configuration, and their molecular conformation were the same. The thermostability analysis showed that the characteristic decomposition temperature of water-soluble yeast β-glucan and yeast β-glucan were 356 ℃ and 360 ℃, respectively. The final decomposition temperature was 740 ℃ and 780 ℃, respectively. The difference in thermal stability between the water-soluble yeast β-glucan and the original yeast β-glucan was not significant.
  • LU Rong-rong et al
    Food and Fermentation Industries. 2018, 44(3): 135. https://doi.org/10.13995/j.cnki.11-1802/ts.015670
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    In order to compare the effects of soy proteins and traditional fining agents on wine clarification and quality, 'Cabernet Sauvignon' dry red wine was selected as test material to study influence of five fining agents (soy protein, gelatin, bentonite, casein and PVPP) on clarity degree, physical and chemical indexes, color, phenolic and volatile compounds of sample wine. The results showed that transmittance of sample wines was increased and color intensity was decreased significantly after fining process (p<0.05). Total acids were decreased by -0.01-0.12 g/L, and volatile acids were increased by 0-0.06 g/L, but residual sugar, alcohol and hue had no significant change. Meanwhile, concentrations of total phenols, tannins, total anthocyanins, monomeric anthocyanins, polymeric anthocyanins and colored anthocyanins in treated sample wines were all lower than those of controls. And with increased dose of fining agents, their concentrations were decreased (except for polymeric anthocyanins). Among them, PVPP and gelatin had great influence on color and total anthocyanins of sample wines. In addition, aroma components in the wine samples were decreased after clarification. However, contents of volatile compounds especially esters in sample wine treated with soy proteins were obviously higher than those in other groups. In conclusion, high clarification degree, good color and aroma of sample wines treated with soy proteins of 200 mg/L indicated that soy protein could be used for the clarification of 'Cabernet Sauvignon' dry red wine.
  • WU Hao et al
    Food and Fermentation Industries. 2018, 44(3): 146. https://doi.org/10.13995/j.cnki.11-1802/ts.015501
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    Using pomegranate and medlar as the main raw material, the fermentation conditions were optimized by response surface method based on single factor test. Pomegranate wolfberry juice ratio, yeast inoculation quantity, and fermentation temperature were chosen as the independent variables. The alcohol content of pomegranate-medlar wine was chosen as the response value. The Box-Behnken central composite experimental design mathematical model was used to carried out response surface analysis. The results showed that the surface fitting of the regression equation was good. Under the optimal fermentation conditions with fermentation temperature of 28 ℃,ratio of pomegranate to wolfberry of 2∶1, and yeast inoculum of 3% conditions, the alcohol content reached 9.94%vol, which was consistent with the predicted model value of 9.93%vol. This wine had pure and thick fragrance, pleasant taste, shiny red brown color, and high transparency. Optimization of the fermentation process of pomegranate and wolfberry wine by response surface methodology is significant.
  • GAO Hui-juan et al
    Food and Fermentation Industries. 2018, 44(3): 151. https://doi.org/10.13995/j.cnki.11-1802/ts.014590
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    Selenium-enriched of Lyophyllum decastes mycelium was cultured in 20L fermenter as raw material in this study. The extraction process of selenium polysaccharide from mycelium of Lyophyllum decastes was optimized and the structure of refined polysaccharides were characterized by IR spectroscopy. One-factors-at-a-time experiments and Box-Behnken experimental design coupled with response surface methodology were carried out to optimize the process parameters of Se-polysaccharides. The results showed that the optimum conditions were: 50 min at 83 ℃, the ratio of solid to liquid 1∶130 (g∶mL), and two times extraction. Under the above conditions, the extraction rate of polysaccharide was 44.62%, which was 0.02% more than predicted, and the selenium content achieved 31.9 μg/g in polysaccharide. After purifying, the refined polysaccharides were characterized by IR spectroscopy. The result showed that the water soluble polysaccharides were pyranopolysaccharide linked by β-glycosidic. The primary structure of water-soluble polyaccharides was not changed, but absorption peaks of pyranring was changed. This further experiment demonstrated that Se participated in the synthesis of Se-polysaccharides during the cultivation of Se-enriched Lyophyllum decastes mycelium.
  • HONG Qing-yue et al
    Food and Fermentation Industries. 2018, 44(3): 159. https://doi.org/10.13995/j.cnki.11-1802/ts.015178
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    Peony seed were extracted by ultrasonic-assisted extraction to produce peony seed oil. The influence of extraction reagent, time, temperature, ultrasound power and liquid-solid ratio on the peony seed oil extraction rate were studied by single factor and orthogonal test. The optimum process conditions of ultrasonic-assisted extraction of peony seed oil was obtained and the composition of peony seed oil fatty acid was analyzed by Gas chromatography. The results showed that the best extraction conditions of ultrasonic-assisted extraction of peony seed oil were: extraction reagent N-hexnane, extraction time 50min, extraction temperature 50 C, ultrasound power 140W, and liquid-solid ratio 1∶15 (g∶mL). Under the above conditions, the peony seed oil extraction yield was (26.11±0.01)%. Gas chromatography analysis showed that the main composition of peony seed oil is palmitic acid, stearic acid, oleic acid, linoleic acid and linolenic acid. Unsaturated fatty acid content was as high as (92.35±0.51) %, linoleic acid and linolenic acid content were (28.80±0.24)% and (41.13±0.09)%, respectively. Peony seed oil is a high nutritional edible oils.
  • XIE Guo-fang et al
    Food and Fermentation Industries. 2018, 44(3): 165. https://doi.org/10.13995/j.cnki.11-1802/ts.014162
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    In order to understand the effect of the size on the quality characteristics of blueberry, blueberries were divided into large, medium, and small based on the weight. The comparison of physical-chemical properties, nutritional composition, antioxidant activity, juice produce ability and volatile compounds were analyzed. The results indicated that the effects of fruit size on varieties and indexes were different, the juice yield were decreased with the fruit size decreasing for all four cultivars. However, fruit size has little effect on fruit index, water content, sugar, TSS, and fruit juice color. The total phenol content of medium fruit was significantly lower than that of large fruit and small fruit. The volatile compounds of juice of Gardenblue is higher than the other three cultivars, and the fruit size has some influence on it without an obvious pattern. It can be seen, for cross-planting blueberry orchard, blueberry picking should be separated by the size to avoid different varieties.
  • SHE Wan-wan et al
    Food and Fermentation Industries. 2018, 44(3): 170. https://doi.org/10.13995/j.cnki.11-1802/ts.015264
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    Coolant temperature controlled foam box is widely used in fresh fruits and vegetables package. However, it is found that the increase of the amount of coolant can't improve the quality of fresh fruits and vegetables. The mechanism of the problem was studied. The tip of the lettuce was packed in a foam box with different amount of coolant (100, 400, 800 g) which simulated normal temperature (25 ℃) logistics transport process. The results showed that in the early and middle logistics process, each treatment group could maintain low weight loss rate, good color and nutritional quality (chlorophyll content, vitamin C content); but at the end of the logistics process, the respiration of lettuce consumed O2 and produced CO2 which caused gas damage and decay eventually. Coolant controlled temperature foam box has obvious stage effect on the preservation of lettuce; it can be divided into three stages: low temperature-high humidity, modified atmosphere and gas injury. Increasing the amount of coolant used in the insulating package is beneficial to maintain the quality of lettuce tip at the early stage of the logistics process, but the effect of prolonging the shelf life is limited. The main factor that restricted the preservation effect of the coolant controlled temperature foam box is the anaerobic respiration caused by the change of gas composition in the box.
  • LI Chun-xia et al
    Food and Fermentation Industries. 2018, 44(3): 179. https://doi.org/10.13995/j.cnki.11-1802/ts.014443
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    The effect of selenium nutrient solution were studied on nutrition quality and antioxidant function of blueberry, so as to provide evidence in developing new natural function food. Solution of selenium nutrient was sprayed on the leaves of blueberry at the late stage of fruit setting and the fruit formation. The fruit nutrition qualities of blueberry, such as nutrient composition, function elements and superoxide dismutase (SOD) activity were analyzed by spectrophotometric method, kjeldah method, atomic fluorescence photometer method and QRS method. In order to study the effects of organic selenium blueberry on ROS in cells, the HepG2 cell were pretreated with organic selenium blueberry before exposure to DCFH-DA, and then the variation of ROS was detected using laser scanning confocal microscope. For the antioxidant level experiment of organic selenium blueberry in vivo, the mouse was fed with organic selenium blueberry for one month. Then the blood and liver of mice were collected for analysis. The results showed that compared with the water and sodium selenite groups, the content of protein, soluble sugar,vitamin C, total phenolics, flavone, selenium, and the activity of SOD raised significantly treated with selenium solution. The content of vitamin E and anthocyanidin sprayed selenium nutrient solution was also improved in a certain degree. The increase of ROS was obviously inhibited by organic selenium blueberry. Comparing to control group, When the mouse were feeded with organic selenium blueberry or selenium blueberry, the content of MDA was obviously reduced to 51.53 % or 29.81%, the activity of SOD was obviously increased to 45.93% and 29.62%, and the activity GSH-Px was increased to 55.94% or 38.02%. The influence of antioxidant enzymes by organic selenium blueberry was greater. Our results indicated that organic selenium blueberry has a significant effect against oxidative damage by nutrition elements optimization and immune function enhancement.
  • CAO Sen et al
    Food and Fermentation Industries. 2018, 44(3): 186. https://doi.org/10.13995/j.cnki.11-1802/ts.013569
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    This paper is about to investigate the effect of ethylene adsorbent coupling with 1-MCP on the postharvest storage quality and after-ripening textures of 'Guichang' Kiwifruit. The best taste samples were obtained by naturally ripen. The harvest fruits were treated by different MCP treatments (0.25 μL/L 1-MCP, 0.5 μL/L 1-MCP, 2%ethylene adsorbent+0.25 μL/L 1-MCP), the control was no treatment. All indexes were tested regularly after 120 d storage. Optimizing treatment was obtained by principal component analysis and compared with best-taste sample(M0). The results show that the treatment of 2% ethylene adsorbent coupling with 0.25 μL/L1-MCP significantly inhibited fruit rotting rate, delayed the decreasing of nutritional quality and significantly decreased the concentration of ethylene in fruit micro-environment. The treatment of 0.5 μL/L 1-MCP can reduced respiration rate and ethylene production rate. The principal component analysis shows that the best texture close to M0 is sample M11-ethylene adsorbent Coupling 0.25 μL/L 1-MCP (6 d), sample M8-0. 5 μL/L 1-MCP (6 d), and sample M12-ethylene adsorbent Coupling 0.25 μL/L 1-MCP (9 d) in the order. It is found that ethylene adsorbent coupling with 0.25 μL/L 1-MCP could maintain the best after-ripening textures of Kiwifruit. Therefore, the postharvest 2% ethylene adsorbent coupling with 0.25μL/L1-MCP treatment is the best in fresh-keeping "Gui Chang" Kiwifruit.
  • WANG Chun-yan et al
    Food and Fermentation Industries. 2018, 44(3): 194. https://doi.org/10.13995/j.cnki.11-1802/ts.014940
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    Salt content and cooking temperature affect processing characteristics and product quality of ground pork. In this paper, salt (1%, 2% and 3%) and cooking temperature (70 ℃, 80 ℃ and 90 ℃) on color, cooking yield, texture and rheological properties of ground pork were studied. The L* value, cooking yield, hardness, springiness and cohesiveness of ground pork increased significantly with the increase of salt content (p<0.05), but b* value was declined. From 25 ℃ to 95 ℃, the energy modulus (G′) of ground pork with 3% of salt was the highest. At the same salt content, the cooking yield and springiness showed the highest at 70 ℃, then decreased with temperature increasing. The b* value and cohesiveness was increased significantly with temperature (p<0.05). The G′ had the highest value at 89 ℃. Overall, appropriate salt content and cooking temperature can improve the quality of ground pork.
  • ZHANG Hai-long et al
    Food and Fermentation Industries. 2018, 44(3): 199. https://doi.org/10.13995/j.cnki.11-1802/ts.014972
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    Three kinds of different polar solvents were used to obtain Jietan tea extracts by ultrasonic-assisted extraction. A systematic study on antioxidation and frying properties of Jietan tea extracts on edible oil was tested by the Schaal oven method and frying test. The results showed that three kinds of extracts all showed certain antioxidative activities and the best one was the ethanol extract. All extracts were better than synthetic BHT, and some performances such as p-anisidine value 36.55 and acid value 1.22 KOHmg/g were even better than that of synthetic antioxidant TBHQ p-anisidine value of 50.80 and acid value of 1.52 KOH mg/g.
  • WANG Ling-zhao et al
    Food and Fermentation Industries. 2018, 44(3): 204. https://doi.org/10.13995/j.cnki.11-1802/ts.015404
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    The degradation products of polysaccharide can overcome its limitations including poor solubility and low bioavailability. In this work, several problems of the degradation of water-insoluble polysaccharide from Porphyrayezoensis by hydrogen peroxide were investigated, including the influence factors and the high performance liquid chromatography, infrared spectrum; ultraviolet spectrum, protein content and total sugar content of products. The optimal conditions of polysaccharide degradation were determined to be hydrogen peroxide concentration 1.8%, temperature 60 ℃, and time 6.0 h. The products contained three oligosaccharides, which might be disaccharide, disaccharide derivative, or oligosaccharides with polymerization degree ≥3. The main products were pyranose oligosaccharides with β-glucosidic bonds, sulphuric groups, and 3, 6-anhydrogalactose structures, and the products contained 2.12% protein and 93.66% total sugar.
  • CHENG Chao et al
    Food and Fermentation Industries. 2018, 44(3): 209. https://doi.org/10.13995/j.cnki.11-1802/ts.015003
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    In this article, the influences of three kinds of cooking methods such as high-pressure pot, microwave ovens and induction cooker on the flavor composition of Pogostemon auricularius (L.) Kassk were analyzed by HS-SPME-GC-MS and electronic nose. The results were as the following, the main volatile components were alkanes, acids and esters. The flavor components were changed after cooking. Compared with the control group, the alkanes components were dropped about 20 times after cooking by high-pressure pot and microwave ovens, and reduced half by induction cooker. The esters contents were increased about two times after cooking by high-pressure pot and microwave ovens and had a better fresh smell. The e-nose results showed the volatile flavor before and after cooking can be well distinguished.
  • YANG Qing-qing et al
    Food and Fermentation Industries. 2018, 44(3): 215. https://doi.org/10.13995/j.cnki.11-1802/ts.015346
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    Barley biscuits product doesn't have many different kind of tastes, and its rough taste affects the quality of the biscuits. Therefore, blueberry barley biscuit formulation was studied. The effect of the amounts of barley powder (67%-100%), blueberry paste (25%-58%), sugar (4%-21%) and water (13%-29%) on the quality of the biscuits were inveistigated . The results showed that the sensory scores of biscuits initially increased with the amount of highland barley powder, blueberry jam, granulated sugar and water, but then decreased. The quality of the barley biscuits was improved when the amount of blueberry sauce was 42%-50%. In addition, the order of factors affecting the quality of biscuits was: water>white sugar> barley powder>blueberry sauce. Under the optimal conditions of biscuit formulation, the appearance, color, flavor, taste and physicochemical properties of the obtained biscuit were acceptable. This study provides a reference for the development of highland barley related products.
  • FU Shi-qian et al
    Food and Fermentation Industries. 2018, 44(3): 220. https://doi.org/10.13995/j.cnki.11-1802/ts.016187
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    A novel detection method had been established to detect Cronobacter sakazakii by Loop-mediated isothermal amplification method coupled with an electric-free heater using calcium oxide and water to provide heat through the LAMP processing. Meanwhile, specific primers were designed based on ITS sequences of Cronobacter sakazakii for the rapid, efficient point-of-care testing on Cronobacter sakazakii of powered infant formula. Finally, the results were determined by agarose gel electrophoresis. Sensitivity of the above method was determined and this non-electric heating detection system was compared with the traditional electric heating method. At the same time, sensitivity experiments were performed at different ambient temperatures. The results showed that the sensitivities of Cronobacter sakazakii in the pure cultures were both 2.6×102 CFU/mL by using two types of technologies while those in artificial contaminated milk were both 4.2×102 CFU/g. This non-electric heating test system had good stability and the sensitivity of 102 CFU/g at environment temperature of 4-37 ℃.
  • LIU Yang et al
    Food and Fermentation Industries. 2018, 44(3): 226. https://doi.org/10.13995/j.cnki.11-1802/ts.016165
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    Vancomycin resistant genes of strain Enterococcus faecalis EC-12 were screened in this study. PCR specific for seven typical genes vanA, vanB, vanC1, vanC2/C3, vanD, vane and vanG were carried out. Results showed that all of them were negative in strain EC-12. This specific PCR method could be used to detect vancomycin-resistant Enterococcus sp.
  • QIAN Shu et al
    Food and Fermentation Industries. 2018, 44(3): 230. https://doi.org/10.13995/j.cnki.11-1802/ts.016018
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    An electronic nose system based on flow modulation in the case of variable flow rates was designed herein to improve the recognition accuracy and shorten the detection time. The gas response range of sensors to different components and concentrations was maximized by changing the intake flow rate. The age of Chinese yellow wine was classified through adaptive principal component analysis(AD-PCA)to verify this system. The results from AD-PCA were compared with those from support vector machine(SVM)and back-propagation neural network(BPNN). The experimental results showed that among these 5 different ages of Chinese yellow wine, the average correct classification rate of AD-PCA was 93.6%, and that of SVM and BPNN was 92% and 100%, respectively. It was proved that the system could quickly classify the wine age on the basis of a higher accuracy rate, and can shorten the detection time compared with the fixed flow rate.
  • DENG Da-chuan et al
    Food and Fermentation Industries. 2018, 44(3): 235. https://doi.org/10.13995/j.cnki.11-1802/ts.014403
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    In order to determine flavors (hexanal and hexanoic acid) in rabbit meat, gas chromatography coupled with headspace sampling technique was developed. Hexanal and hexanoic acid in samples were extracted by static headspace sampler, then separated with Rtx-Wax capillary column and detected by FID. Retention time of the peaks was used for qualitative analysis, and external standard method was used for quantitative analysis. The results showed that the HS-GC-FID method had a good linearity over the concentration range of 5-100 μg/kg for hexanoic acid and hexanal with correlation coefficients of 0.999 3 and 0.999 5, respectively. The limits of detection (LOD) for hexanal and hexanoic were 1.49 μg/kg and 2.57 μg/kg, respectively, and the limits of quantification (LOQ) were 4.5 μg/kg and 7.78 μg/kg, respectively. The recoveries were in the range of 89%-98% for hexanal, and 90%-96% for hexanoic acid; the relative standard deviations (RSD) were 3.81% for hexanal and 2.41% for hexanoic acid. Hexanal detected in rabbit meat was in the range of 49.62-67.73 μg/kg; Hexanoic acid detected in rabbit meat was in the range of 10.32-14.92 μg/kg. OAV analysis showed that hexanoic acid and hexanal have a direct effect on rabbit meat odor.
  • SHI Miao-miao et al
    Food and Fermentation Industries. 2018, 44(3): 241. https://doi.org/10.13995/j.cnki.11-1802/ts.015059
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    In order to study the changes of the crystal structure of potato starch, we prepared acid hydrolyzed starch, B-type crystallite starch and V-type compounds of potato starch. The Raman spectroscopy was used to analyze the structure of those starch. By comparing different crystalline structure of Raman spectrum, it can be seen that the peak intensity gradually reduced. The relative peak area is also changed. The formation of complex makes the double helix conversion into the single helix structure. The position of characteristic peaks in different compound were almost the same, but the intensity of peaks showed a big difference.
  • YANG Xiao et al
    Food and Fermentation Industries. 2018, 44(3): 247. https://doi.org/10.13995/j.cnki.11-1802/ts.015045
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    An electronic nosed based nondestructive method was established for detecting frozen pork quality as well as determining whether the pork had been refrigerated at 4 ℃ for a long time before frozen. 5 cm thick square frozen pork was added into a 100 mL beaker and sealed by a membrane; PEN3 electronic nose was used to detect the gas in the upper beaker. The data were analyzed by principal component analysis and variance analysis to understand the distribution of multiple data. Secondly, the prediction model of frozen pork and whether it has been refrigerated at 4 ℃ for a long time before the frozen storage was established. There are significant differences of electronic nose data of frozen pork at different storage as well as whether it had been refrigerated at 4 ℃ for a long time. Both linear discriminant analysis and neural network algorithm based on multi-layer perceptron can establish a successful prediction model. The predictive model based on neural network algorithm has better prediction. The detection method is simple, fast and efficient and nondestructive. It lays a technical foundation in order to enhance the supervision and management for frozen meat.
  • WANG Cheng-xiang et al
    Food and Fermentation Industries. 2018, 44(3): 253. https://doi.org/10.13995/j.cnki.11-1802/ts.014159
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    The influence of three kinds of thickening agents including carrageenan, gellan gum, microcrystalline cellulose amount on the stability of oat beverage were investigated by two rapid stability analysis instrument LUMiSizer and Turbiscan, combined with standing observation, centrifugal precipitation, and average particle size. The results showed good inhibition effect of sedimentation with 0.04% of microcrystalline cellulose. In terms of stability prediction, the LUMiSizer and Turbiscan have their own advantages and shortcomings. The centrifuge acceleration of LUMiSizer showed the internal system combination, such as flocculation. The static light scattering of Turbiscan showed the internal components migration under actual gravity, and precisely describes the extent of precipitation and floating.
  • SHI Xiao-ya et al
    Food and Fermentation Industries. 2018, 44(3): 260. https://doi.org/10.13995/j.cnki.11-1802/ts.015791
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    An indirect competitive enzyme linked immunosorbent assay (ic-ELISA) using monoclonal antibody was developed to determine furazolidone metabolite. Made 3-{[(4-carboxyphenyl) methylene]amino}-2-oxazolidinone (CPAOZ) was conjugated with Ovalbumin (OVA) using N-hyduoxysuccinimide ester method to obtain CPAOZ-OVA. Then the influences of several physicochemical factors such as dilution ratios of CPAOZ-OVA and monoclonal antibody, blocking solution formulation, competitive reaction time, incubation time of HRP-IgG, chromogenic reaction time were optimized and the sensitivity, specificity, accuracy and precision of the method were evaluated. In the optimized system, the dilution ratios of CPAOZ-OVA and monoclonal antibody were 800-fold and 6400-fold respectively; blocking solution contained 1% skimmed milk; competitive reaction time was 60 min; incubation time for HRP-IgG was 45 min; chromogenic reaction time was 15 min. Based on the established standard curve, the linear equation for ELISA was set as Y=-0.253X+1.336(R2=0.995). IC50 was 2.015 ng/mL and linearity range was 0.131-30.911 ng/mL. The recovery rate of negative pork samples was 84.8%-90.3%. The coefficients of variation in intra-assay and inter-assay were 3.3%-4.1% and 4.6%-6.7% respectively. The method was proved to have excellent specificity, accuracy, precision and reproducibility, thus could be applied to rapid detect AOZ content in animal-derived food.
  • ZHAO Xing-he et al
    Food and Fermentation Industries. 2018, 44(3): 266. https://doi.org/10.13995/j.cnki.11-1802/ts.016423
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    In order to determine the key odorants in Beijing dry yellow soybean sauce (BDYSS), the volatile constitutes in BDYSS were isolated by solid-phase microextraction, and the isolate was analyzed by gas chromatography-mass spectrometry-olfactometry(GC-MS-O). The structures of volatile components separated by GC were determined by MS, and conformed by comparison of retention indexes (RIs) of separated constituents with the RIs reported in the literature and by comparison of the retention times and odor characteristics of some separated constituents with those of authentic samples. A total of 53 volatile compounds, of which 20 compounds had odor activity, were identified. Their concentrations were determined by using 2-octanol as internal standard. The odor activity values (OAV) of aroma-active compounds who gave respond signals in total ion chromatogram were calculated. Seven key odorants in BDYSS were identified by OAV, they were 3-methylbutanal, dimethyl trisulfide, 2-methoxyphenol, benzeneacetaldehyde, ethyl butanoate, nonanal and 3-methylbutanoic acid.
  • QI Xiao-hui et al
    Food and Fermentation Industries. 2018, 44(3): 273. https://doi.org/10.13995/j.cnki.11-1802/ts.015364
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    Proteins play a crucial role in the quality of Chinese rice wine, which are mainly existed in the form of amino acids and peptides. The content of amino acid nitrogen is an important quality index contributing to their nutritive value. The non-biological turbidity in Chinese rice wine mainly caused by proteins is a major problem in the industry. Chinese rice wine is gradually got increasing attention due to its nutrition and health benefits, and more and more researches on Chinese rice wine proteins have been conducted and most of them are focus on haze proteins. In this review, the research status, problems, and future research direction of Chinese rice wine protein were discussed. The composition and source of bottled rice wine protein, as well as the specific removal of precipitated proteins such as dimeric-α-amylase inhibitors should be a research direction in future.
  • LIANG Ya-nan et al
    Food and Fermentation Industries. 2018, 44(3): 280. https://doi.org/10.13995/j.cnki.11-1802/ts.014752
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    Apple processing industry is a major fruit processing industry in the world. Apple juice is the most important and productive production in apple processing industry. The color of apple juice is a critical quality index used in consumers' judgement of the quality of apple juice. The color is mainly affected by its browning in the process. According to its browning mechanism, browning of apple juice mainly includes enzymatic browning and non-enzymatic browning. Due to the high content of phenolic compounds and high activity of polyphenol oxidase in raw apples, enzymatic browning is the main browning cause. Therefore, the control of apple juice browning, especially enzymatic browning, is of great significance to protect its quality and improve consumers′ acceptance. On the basis of an extensive review of relevant literature, this paper summarizes the mechanism of apple juice browning including enzymatic browning and non-enzymatic browning, as well as browning control technology including physical control technology, browning inhibitor and other control technologies. Moreover, the problems in this field and the future development direction are also proposed.
  • WANG Xin-yue et al
    Food and Fermentation Industries. 2018, 44(3): 287. https://doi.org/10.13995/j.cnki.11-1802/ts.014332
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    In recent years, with the increasing demand of fresh berry, berry industry in China developed rapidly. However, fresh berries are perishable and hard to store. Therefore, the preservation of fresh berry had become the primary issue which limits its development. As a natural and nutrient preservation technology, calcium treatment meets the requirements for food safety. In order to provide reference for the preservation of berries in the future and promote healthy development of berry industry, the effects of different calcium treatments on postharvest quality of berry fruit was summarized in this paper.