15 June 2026, Volume 52 Issue 11
    

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  • LU Xingmiao, ZHU Qingquan, HOU Feihong, YANG Chaohao, REN Xiaofei, LU Fuping, LIU Fufeng
    Food and Fermentation Industries. 2026, 52(11): 1-9. https://doi.org/10.13995/j.cnki.11-1802/ts.044229
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    α-L-Rhamnosidase is a glycosidase capable of specifically hydrolyzing terminal rhamnose residues in flavonoids, widely used in food and pharmaceutical industries.However, its industrial application faces challenges of high cost and poor stability.Thus, this study optimized fermentation conditions and employed immobilization techniques to enhance enzyme activity and stability using a previously constructed recombinant Pichia pastoris strain GS115/pPIC9K-Rha-gcw61.The results showed that using BMGY as the medium, with 1.59% glycerol, 2.49% (NH4)2SO4 (mass fraction), and 2.90% inoculum (volume fraction), yield the highest enzyme activity of 170 U/mL in the fermentation broth.Bioreactor cultivation further increased enzyme activity to 1 380 U/mL after 86 h at 30% dissolved oxygen, which is 8.12-fold compared to shake-flask cultures.The enzyme solution was used to convert rutin into isoquercitrin, the conversion rate exceeded 90% within 24 h for rutin mass concentrations below 75 g/L, while higher concentrations (100, 125, and 150 g/L) required 48 h to achieve similar conversion rates.The optimal temperature and pH for the immobilized α-L-rhamnosidase were 60 ℃ and 5.0, respectively.Furthermore, the immobilized enzyme retained more than 70% of its initial activity after storage at 4 ℃ for 50 days, indicated excellent storage stability.These findings provide both technical and theoretical foundations for the industrial production of α-L-rhamnosidase and efficient biotransformation of high-value flavonoid compounds.
  • DONG Yunpeng, CAI Jun
    Food and Fermentation Industries. 2026, 52(11): 10-17. https://doi.org/10.13995/j.cnki.11-1802/ts.044396
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    The yield of chitosan oligosaccharides (COS) produced by enzymatic hydrolysis can be enhanced by optimizing both the enzyme characteristics and the selection of suitable substrates.In this study, a chitosanase with an approximate molecular weight of 40 kDa was purified from the fermentation broth of a Bacillus strain using ammonium sulfate precipitation, DEAE FF 16/10, and Superdex 75 10/300 GL column chromatography.The enzyme exhibited optimal activity at pH 5.5 and remained stable between pH 5.0 and 7.0.Its highest activity was observed at 55 ℃, with stability maintained from 30 ℃ to 55 ℃.Thin-layer chromatography analysis revealed that the enzyme hydrolyzed chitosan and COS with a degree of polymerization greater than four, producing primarily (GlcN)2, (GlcN)3, and (GlcN)4.Additionally, two commercially available chitosan powders were utilized to prepare a series of substrates, which were characterized by Fourier transform infrared spectroscopy, X-ray diffraction and gel permeation chromatography.Post-reaction analysis indicated that the chitosan substrate with approximately 80% deacetylation and a molecular weight of 100 000 yielded the highest concentration of oligosaccharides:(GlcN)2 at 1.112 mg/mL, (GlcN)3 at 1.636 mg/mL, and (GlcN)4 at 0.824 mg/mL.These findings suggest that the enzyme’s efficiency is influenced by the substrate’s molecular weight and deacetylation degree.This study provides valuable insights for optimizing enzymatic processes in the industrial production of COS.
  • LU Xingmiao, QIN Lihong, ZHAO Danyang, LU Fuping, LIU Fufeng
    Food and Fermentation Industries. 2026, 52(11): 18-26. https://doi.org/10.13995/j.cnki.11-1802/ts.044359
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    Surfactin is a lipopeptide biosurfactant with good physicochemical properties and biological activity.However, natural bacterial strains exhibit low surfactin yield and high costs, severely limits its industrial application.Therefore, Bacillus subtilis ATCC 21332 served as the starting strain, homologous recombination and CRISPR-Cas9 gene editing, combined with fermentation condition optimization, were employed to enhance surfactin expression and yield.Homologous recombination was used to replace the native PsrfA promoter with three strong promoters.The highest yield was achieved with Pveg promoter, reached 3.59 g/L, which was 28.2% higher than the wild type.To further increase yield, seven key genes were knocked out by CRISPR-Cas9, including srfA synthase negative regulatory factors (phoP, codY, sinI), spore synthesis key genes (spoIIIE, spoIVB), and biofilm synthesis key genes (sipW, tasA).The results showed that knocking out the phoP gene resulted in the greatest increase, reached 4.17 g/L, which was 49% higher than the wild-type.Fermentation optimization identified the following optimal conditions:4% inoculum (volume fraction), 30 mL/250 mL loading, 37 ℃, initial pH 7, and addition of 2 g/L glutamic acid.Under these conditions, engineered strains achieved approximately 1.5-2.0-fold improvements in surfactin production.The highest yield was obtained from B.subtilis ATCC 21332- Δ sipW, reached 5.98 g/L after 48 h of fermentation, representing a 113.7% increase over the wild type.This study offers theoretical support for elucidating the biosynthetic mechanism of surfactin and lays a foundation for industrial production and application.
  • LIU Mengyuan, WU Xia, REN Juan, ZHA Jian
    Food and Fermentation Industries. 2026, 52(11): 27-35. https://doi.org/10.13995/j.cnki.11-1802/ts.045447
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    Caffeic acid is an important phenolic acid compound widely used in food, biomedical, and nutraceutical industries.Its microbial biosynthesis receives increasing attention, and the highest production titer is achieved in recombinant Escherichia coli.However, in all the reported E.coli systems, the pathway genes for caffeic acid biosynthesis are expressed episomally, requiring antibiotic supplementation during fermentation.These lead to strain instability, high production costs, and dissemination of antibiotic-resistance genes.To this end, the biosynthetic pathway genes were inserted into E.coli genome for the construction of an integrative strain capable of de novo biosynthesis of caffeic acid from glucose.Via comparison of hydroxylases from various sources, comparison of various gene integration sites, and optimization of gene copy number, a three-copy strain CA8 was obtained via gene integration in three sites, and the corresponding titer of caffeic acid in shake flasks reached 488 mg/L.Further optimization of fermentation media, inoculation size, and inducer concentration promoted the titer to 598 mg/L in shake flasks.Under the optimal condition, this recombinant strain produced 1.55 g/L of caffeic acid in a 5 L bioreactor using fed-batch fermentation.This study provides guidance to efficient microbial biosynthesis of caffeic acid and other plant natural products.
  • HUANG Zheng, ZHANG Zhiyi, YE Wei, GU Yang
    Food and Fermentation Industries. 2026, 52(11): 36-44. https://doi.org/10.13995/j.cnki.11-1802/ts.044087
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    Clostridium is a group of Gram-positive anaerobic bacteria widely distributed in natural environments and living organisms, playing a significant role in human health, biomedicine, and industrial production.However, due to the relatively limited molecular genetic tools available for Clostridium, the exploration of key functional genes, elucidation of molecular mechanisms, and the construction of high-performance engineered strains remain challenging.In recent years, transposon technology has emerged as an efficient genetic manipulation tool in research on Clostridium species, providing effective technical means for gene delivery, genome editing, functional genomics, and metabolic optimization.This article systematically reviews recent advances in transposon-related technologies for Clostridium, discusses their current applications and potential in gene function identification and strain engineering, and offers perspectives on future technological developments.The aim is to provide insights and references for both fundamental research and industrial applications of Clostridium.
  • LI Liuruolan, ZHAO Yurong, WANG Yi, YU Xihua, LU Wenwei, WANG Hongchao, WU Wenjun
    Food and Fermentation Industries. 2026, 52(11): 45-52. https://doi.org/10.13995/j.cnki.11-1802/ts.044104
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    Prediabetes is a chronic metabolic disease with high prevalence and may develop into type II diabetes.Thus, safe and effective early intervention strategies are urgently needed.Probiotics have great potential in improving glucose metabolism, but the specific effects and potential mechanisms of compound probiotics in prediabetes remain to be further explored.Therefore, in this study, Bifidobacterium longum subsp. longum CCFM1113 and Bifidobacterium adolescentis CCFM1386 were compounded to study their effects on prediabetes mice induced by high-fat diet combined with streptozotocin (STZ).The results showed that compared with the model group, the compound probiotics significantly reduced weight gain, improved glucose tolerance, modulate glucose metabolism, and reduced insulin resistance;In addition, through histopathological analysis, it was found that the compound probiotics had protective effects on the liver and pancreas of prediabetes mice, and also significantly reduced the contents of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in the liver of mice.Metagenomic sequencing of gut microbiota showed that the compound probiotics can increase microbiota diversity and help restore microbiota structure, promoting the growth of beneficial bacterial related to the regulation of metabolic disorders.The above results indicate that the compound probiotics can alleviate the symptoms of prediabetes mice by regulating metabolism and promoting the growth of beneficial bacteria.
  • YANG Haixin, LI Tong, WU Junrui, XIA Suning, AN Feiyu, SHI Haisu, GUO Jia, XUE Ruixia, WU Rina, ZANG Jian
    Food and Fermentation Industries. 2026, 52(11): 53-64. https://doi.org/10.13995/j.cnki.11-1802/ts.044052
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    The global health burden of alcohol-induced liver injury is increasing, necessitating effective hepatoprotective strategies.This study employs Lactiplantibacillus plantarum FS5-5 as a fermenting agent to bioferment Pueraria lobata extract.The optimization of fermentation parameters resulted in the production of a fermented product, fermented pueraria lobata extract by Lactiplantibacillus plantarum FS5-5 (FPE5).The results indicate that fermentation significantly enhances the flavonoid content, total phenolic compounds, and antioxidant capacity of FPE5.Animal studies demonstrate, a high dose of FPE5 (FH) demonstrated significant efficacy.FH prolonged the time to the loss of righting reflex, shortened the recovery time, and lowered the serum ethanol concentration.FH effectively reduces serum alanine aminotransferase and aspartate aminotransferase activities, ameliorates hepatic histopathological damage, while concurrently enhancing hepatic alcohol dehydrogenase and aldehyde dehydrogenase activities.It also elevates total superoxide dismutase activity, reduces the content of malondialdehyde, increases the content of glutathione, and suppresses pro-inflammatory cytokine expression.Mechanistic investigations indicate that FPE5 mitigates oxidative stress by downregulating Cyp2e1 gene expression and modulating the Keap1/Nrf2/HO-1 signaling pathway, thereby exerting hepatoprotective and anti-alcoholic effects.This study confirms that fermentation with Lactiplantibacillus plantarum can significantly enhance the antioxidant capacity and hepatoprotective effects of Pueraria lobata, providing a theoretical foundation for the development of functional products aimed at alcohol detoxification and liver protection.
  • WANG Hongyan, HAN Min, LIU Rui, CHENG Kun, MU Shuaicheng, SONG Zhiquan, LIU Yiru, XU Jin
    Food and Fermentation Industries. 2026, 52(11): 65-73. https://doi.org/10.13995/j.cnki.11-1802/ts.044978
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    Constipation has become an important factor affecting quality of life, and probiotics may improve constipation symptoms by modulating the gut microbiota.However, probiotic functions exhibit high strain specificity, necessitating evaluation at the strain level.This self-controlled clinical trial aimed to investigate changes in gut microbiota and functional pathways in constipated individuals following intervention with Bifidobacterium animalis subsp.lactis BL-99.This study enrolled 21 subjects suffering from constipation to undergo the trial, administering fermented yogurt containing BL-99 (1×1011 CFU/d) for a 4-week intervention period followed by a 2-week follow-up.This study collected patient assessment of constipation-symptom and fecal samples.Fecal samples underwent metagenomic sequencing and genome reconstruction for analysis using a self-controlled design.The results indicates that BL-99 intervention effectively improves constipation symptoms in participants, with effects persisting for at least two weeks post-intervention.The average relative abundance of Bifidobacterium animalis_B and unclassified Bifidobacterium significantly increase (P<0.05).Differential genes before and after intervention are enriched in KEGG pathways including biosynthesis of amino acids, biosynthesis of cofactors, and ribosome.Analysis of carbohydrate-active enzymes reveals that differential genes are primarily concentrated in glycoside hydrolase family 13, glycosyl transferase family 2, and carbohydrate-binding module family 48.These findings suggest that Bifidobacterium animalis_B and unclassified Bifidobacterium may serve as microbial markers for constipation relief efficacy by BL-99 intervention.BL-99 intervention induces functional alterations in biosynthesis of amino acids pathways and related carbohydrate-active enzymes, providing theoretical support for intervening in constipation.
  • WEI Bingqi, CHEN Liu, ZHANG Jianping, HAO Zailin, LIU Yanxin
    Food and Fermentation Industries. 2026, 52(11): 74-80. https://doi.org/10.13995/j.cnki.11-1802/ts.044047
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    To explore natural and novel functional foods for the prevention and treatment of chronic alcoholic liver injury (CALI), three novel active peptides complexes were developed through rational formulation.Their regulatory effects on liver protection, and inflammation in CALI mice were evaluated, and the underlying mechanisms involving oxidative stress, lipid metabolism, and gut microbiota modulation were investigated.Results showed that all three peptide complexes, particularly the concentrated formulation, effectively alleviated alcohol-induced behavioral changes in CALI mice.They significantly enhanced hepatic alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) activities, reduced liver index, and lowered serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), thereby improving hepatic histopathological damage.Moreover, these complexes downregulated hepatic TNF-α and IL-6 levels, while increasing superoxide dismutase, catalase, and glutathione contents, and reducing malondialdehyde levels.Additionally, they improved lipid profiles, including total cholesterol and triglycerides, and enhanced gut microbiota diversity and abundance.These findings provide a scientific basis for the development of novel functional foods with hepatoprotective and alcohol-metabolism-enhancing properties.
  • XING Minyu, WANG Mimi, ZHANG Yali, LU Xiaoyun, RAN Ganqiao
    Food and Fermentation Industries. 2026, 52(11): 81-91. https://doi.org/10.13995/j.cnki.11-1802/ts.045376
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    To investigate the regulatory effects of psicose-fructose syrup on glucose and lipid metabolism disorders and gut microbiota imbalance in diabetic mice, providing evidence for its application as a functional food ingredient.A diabetic mouse model was constructed using a high-energy diet combined with streptozotocin (STZ), and various proportions of D-psicose in psicose-fructose syrup were administered.The food intake, body weight, fasting blood glucose, insulin levels, oral glucose tolerance (OGTT), glycated hemoglobin (HbA1c), blood lipid profile, liver function, and thyroid function were measured.The gut microbiota structure was analyzed using 16S rRNA sequencing.Psicose-fructose syrup significantly reduced fasting blood glucose and HbA1c levels in diabetic mice, improving glucose tolerance and insulin resistance.The improvement in HbA1c and insulin levels was more pronounced with higher D-psicose content.Blood lipid analysis showed that psicose-fructose syrup significantly reduced triglyceride (TG) levels, and the high D-psicose group (DM-HPsi group) further significantly increased high-density lipoprotein cholesterol (HDL-C).Additionally, psicose-fructose syrup significantly lowered the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), alleviating liver damage in diabetic mice.Gut microbiota analysis showed significant dysbiosis in the diabetic mice model, characterized by a decreased Firmicutes/Bacteroidetes ratio, an increased proportion of Gram-negative bacteria, and a higher abundance of harmful bacteria such as Bacteroides and Alistipes.Intervention with psicose-fructose syrup significantly reversed this imbalance, restoring the Firmicutes/Bacteroidetes ratio and significantly increasing the abundance of beneficial bacteria, including Blautia, Faecalibaculum, Romboutsia, Mucispirillum and Dubosiella.Psicose-fructose syrup improves glucose and lipid metabolism disorders and liver damage in diabetic mice, exhibiting significant hypoglycemic, lipid-regulating, and hepatoprotective effects.It also effectively restores the gut microbiota structure, enhancing the abundance of beneficial bacteria, demonstrating a favorable microecological regulation effect.This study provides experimental evidence for the application of psicose-fructose syrup as a functional food in the prevention and treatment of diabetes and metabolic syndrome.
  • XIE Qiurong, HE Shuyu, LIN Jiyan, YE Yuehong, LI Jiapeng
    Food and Fermentation Industries. 2026, 52(11): 92-100. https://doi.org/10.13995/j.cnki.11-1802/ts.045271
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    Black tea, a widely consumed beverage, has garnered increasing attention for its potential anti-inflammatory and gut health regulatory properties.This study aimed to investigate the protective effect of black tea against dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice and its underlying mechanism involving the modulation of gut microbiota.C57BL/6 mice were randomly divided into a control group, a DSS-induced model group, a black tea treatment group [450 mg/(kg·d)], and a positive control mesalazine group [20 mg/(kg·d)].A chronic UC model was established using 2% (mass fraction) DSS solution, followed by the respective treatments.The results showed that, compared with the model group, black tea treatment significantly alleviated histopathological damage in colon tissue and inhibited the expression of pro-inflammatory cytokines IL-1β, IL-6, and TNF-α (P<0.05).Analysis of gut microbiota by 16S rRNA sequencing revealed that black tea treatment significantly restored microbial richness (Chao1 and ACE indices, P<0.05), reduced the relative abundance of harmful bacteria such as Proteobacteria, Gammaproteobacteria, Burkholderiales, Sutterellaceae, Parasutterella, Bacteroides acidifaciens, and Desulfovibrio (P<0.05), while increasing the abundance of beneficial bacteria such as Muribaculaceae and Blautia (P<0.05).Further functional prediction indicated that black tea treatment up-regulated beneficial metabolic pathways related to carbohydrate derivative synthesis and coenzyme regeneration, such as PWY-7315, PWY-6396, METH-ACETATE-PWY, and NAD-BIOSYNTHESIS-Ⅱ, while down-regulating harmful pathways associated with bacterial drug resistance and oxidative stress, such as P23-PWY, P461-PWY, HEMESYN2-PWY, and PWY-7377) (P<0.05).In conclusion, black tea effectively alleviated DSS-induced ulcerative colitis in mice, and its mechanism is closely related to multi-dimensionally remodeling of gut microbiota structure, restoration of microecological balance, and regulation of microbial functional metabolism, thereby synergistically exerting anti-inflammatory and intestinal mucosal protective effects.This study provides experimental evidence for black tea as a potential microecological modulator to assist in managing gut health.
  • WANG Yuxin, SHI Miaoying, WANG Xiaohong, YANG Hui, JIA Xudong
    Food and Fermentation Industries. 2026, 52(11): 101-106. https://doi.org/10.13995/j.cnki.11-1802/ts.045765
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    Deoxynivalenol (DON) is a common mycotoxin present in cereals in China and reduced body weight gain observed in mice was used as the critical effect in its risk assessment.However, its immunotoxicity has garnered increasing attention in recent years.To address limitations such as uncertainties in extrapolating animal data to humans, this study integrated in vitro toxicity data with a physiologically based toxicokinetic (PBTK) model to develop an assessment strategy based on new approach methodologies (NAMs) for assessing the risk of immunotoxic effects from DON exposure among Chinese.A PBTK model for Chinese adults was built and validated using parameters derived from literature and experiments.Subsequently, a PBTK modeling based reverse dosimetry approach was employed to extrapolate in vitro concentrations of DON reported in the literature to in vivo doses, thereby deriving points of departure (PoDs) based on immunotoxicity.Results indicated that PoDs for DON based on immunotoxic endpoints ranged from 2.7 to 282 μg/(kg BW·d), with some PoDs being lower than the PoD underlying the current tolerable daily intake, suggesting that immunotoxicity may serve as a more sensitive toxic endpoint than reduced body weight gain for the risk assessment of DON.This study provides a NAMs-based hazard assessment strategy, providing new insights for food safety risk assessment of DON in China.
  • WANG Jing, ZHONG Qiuping
    Food and Fermentation Industries. 2026, 52(11): 107-115. https://doi.org/10.13995/j.cnki.11-1802/ts.044221
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    This study employed metagenomic technology combined with headspace solid-phase microextraction/gas chromatography-mass spectrometry to investigate microbial community structure and functional characteristics during lychee wine fermentation at 15, 25, and 35 ℃ temperatures, while analyzing microbial associations with flavor compounds.The results revealed that, at the three fermentation temperatures, apart from the artificially inoculated Saccharomyces cerevisiae, the endogenous microorganisms carried by the lychee, such as Zymomonas mobilis, Leuconostoc litchii, L.pseudomesenteroides, L.suionicum, L.citreum, L.mesenteroides, Lactococcus lactis, L.cremoris, and Limosilactobacillus fermentum, were the dominant species during the fermentation process.KEGG functional annotation analysis demonstrated that carbohydrate metabolism, amino acid metabolism, energy metabolism, and nucleotide metabolism pathways dominated in the metabolic process with high abundance levels.The enrichment analysis of metabolic pathways of flavor compounds revealed three major metabolic pathways:galactose metabolism, ascorbate and aldarate metabolism, and biosynthesis of unsaturated fatty acids.Further correlation studies between microbial communities and flavor compounds demonstrated significant associations, with Lactococcus and Lactobacillus fermentum significantly positively correlated with specific flavor compounds.This research elucidates microbial community variations in lychee wine fermentation at three distinct temperatures, along with the impact of key microorganisms on flavor profiles.The findings provide theoretical foundations for optimizing the selection of core microorganisms and determining the optimal fermentation temperature parameters.
  • CHEN Xiaoru, ZHENG Jia, YIN Liguo, LIN Hanrui, WU Shengjie, WEI Mingying, SUI Ming, TANG Xianhua, ZHANG Chongjun, LIU Linpei
    Food and Fermentation Industries. 2026, 52(11): 116-123. https://doi.org/10.13995/j.cnki.11-1802/ts.044415
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    The integration of conventional screening techniques and ARTP mutagenesis presents a prominent advantage for strain improvement in the Baijiu fermentation industry.This study focused on various Daqu samples from Sichuan-producing regions.Through a combined approach of traditional screening and ARTP mutagenesis, high-yield pyrazine-producing strains were successfully bred and systematically characterized.Their practical application in actual Baijiu fermentation using fermented grains was further investigated.Results showed that the initially isolated strain X-23 was identified as Bacillus subtilis via molecular biology methods—a Gram-positive bacterium capable of producing 85.48 mg/kg of pyrazines.After ARTP mutagenesis, the mutant strain M-6 exhibited significant morphological changes under scanning electron microscopy and showed enhanced protein metabolism capabilities.Specifically, gelatin liquefaction and malonate test results turned from negative to positive.Under solid-state sorghum culture conditions, the total volatile compound production by M-6 increased by 140.26% compared to the wild strain, with pyrazine yield reaching 1 240.36 mg/kg-14.51 times higher than that of the original strain.The mutant also produced a more diverse profile of pyrazine compounds.Fermentation validation in actual grain compost further confirmed that inoculation with M-6 led to the detection of dimethyl-pyrazine [(21.37±2.82) mg/kg], trimethyl-pyrazine [(27.23±1.95) mg/kg], and tetramethyl-pyrazine [(60.71±1.42) mg/kg].Thus, M-6 demonstrates excellent pyrazine synthesis capacity in fermented grain systems.This study provides novel microbial resources and a theoretical foundation for enhancing product quality and innovating brewing processes in China’s traditional Baijiu industry.
  • ZHANG Zhengjie, XU Defu, MING Hongmei, XU Qiong, WEN Yue, LIAO Chuan, JIA Junjie, CHEN Zhilin, GAO Feng, LI Wenjin, HE Zhuo, WANG Jiali
    Food and Fermentation Industries. 2026, 52(11): 124-135. https://doi.org/10.13995/j.cnki.11-1802/ts.044391
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    The mixed use of Daqu and bran Qu is an innovative method in liquor-making process, which can improve liquor quality, optimize fermentation efficiency and reduce cost.In this study, the double koji brewing liquor process was taken as the object.The dynamic changes of microbial community structure and volatile flavor substances in fermented grains during fermentation and their correlation mechanisms were systematically analyzed by using headspace solid phase microextraction-gas chromatography-mass spectrometry and high-throughput sequencing technology.The results showed that 114 volatile flavor substances were detected in fermented grains, including 60 esters, 24 alcohols, 9 acids, 11 aldehydes and ketones, and 10 other types, showing a rich flavor spectrum.There were 10 dominant fungal genera (average relative abundance >1%), including Thermomyces, Rasamsonia and Aspergillus, and 11 dominant bacterial genera, including Lactobacillus, Limosilactobacillus and Weissella, were formed during the fermentation process, and their dynamic changes were significantly correlated with the fermentation stage.Microbial network analysis revealed that dominant genera such as Thermoactinomyces, Bacillus and Staphylococcus, and non-dominant genera such as Pseudomonas, Meniscus, and Companilactobacillus all had key regulatory effects on community stability.Correlation analysis showed that Rasamsonia, Lactobacillus and other bacteria were important contributors to the formation of flavor substances.Starch and reducing sugar content were highly significantly positively correlated with the dominant genera in the early stage of fermentation (P<0.001), while moisture and acidity were highly significantly positively correlated with the dominant genera in the late stage of fermentation (P<0.001).This study provides a theoretical basis for the precise regulation and optimization of hyperbolic brewing process, and has important practical value for improving liquor quality and process standardization.
  • YANG Yiqiu, YANG Junjie, MA Yuanmiao, CHENG Wenqian, DU Lijuan, SUN Qinju, WEI Lu, MENG Jianzong, SUN Yizhen
    Food and Fermentation Industries. 2026, 52(11): 136-144. https://doi.org/10.13995/j.cnki.11-1802/ts.043691
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    Sauce-flavored multicolored pickled peppers, a traditional fermented vegetable product from the Lingnan region, are indispensable as accompaniments to porridge, noodles, and meals, known for their salty, umami, crisp texture, and appetizing sour-spicy flavor.Traditionally produced through spontaneous fermentation, this study aims to enhance production efficiency by comparing the effects of inoculated fermentation and spontaneous fermentation on the quality dynamics of multicolored peppers during fermentation, and establishing kinetic models for the process.The results indicated that during fermentation, the total acid and polyphenol contents increased, while vitamin C and reducing sugar contents decreased.The lactic acid bacteria count and nitrite content initially increased and then declined.After 30 days of fermentation, the inoculated fermentation group exhibited a 52.84% reduction in nitrite content, along with increases of 5.75% in total acid, 7.15% in polyphenols, and 146% in vitamin C, compared to the spontaneous fermentation group.Model fitting revealed that the DoseResp model best described microbial growth and product formation in the spontaneous fermentation group, while substrate consumption followed the Logistic model.For the inoculated fermentation group, microbial growth and substrate consumption were optimally fitted by the Logistic model, whereas product formation followed the DoseResp model.All models demonstrated extremely high goodness-of-fit (R2>0.99).Although the quality trends of the pickled peppers were similar between the two fermentation methods, the inoculated fermentation group achieved significantly superior quality.The established kinetic models effectively describe the fermentation process, providing a theoretical foundation for quality control and industrial-scale production of sauce-flavored multicolored pickled peppers.
  • TAN Mei, LU Dan, YE Zhizhuang, CHEN Yunjing, XU Rui
    Food and Fermentation Industries. 2026, 52(11): 145-151. https://doi.org/10.13995/j.cnki.11-1802/ts.043951
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    This study investigated the effects of enzymatic pretreatment combined with fermentation on enhancing butter flavor.Lipase Palatase 20000L (L) and protease NS 37071 (P) pretreated milk fat and protein sequentially.Then, a mixture of lactic acid bacteria (Lactobacillus bulgaricus subsp.bulgaricusStreptococcus thermophilus subsp.thermophilus=3∶2) was used for fermentation (F).GC-MS analysis revealed formation mechanisms of key flavor compounds.Results demonstrated that stepwise enzymatic hydrolysis released flavor precursors (short chain fatty acids and hydrophobic peptides), significantly promoting subsequent flavor enhancement during fermentation.Compared with traditional methods, the combined L→P→F group achieved:processing time reduced to 18 h, 2.8-fold increase in total volatile compounds.Significant increases (P<0.05) in key flavorants:ethyl butyrate, 2-heptanone. The enzymatic-fermentation synergy effectively enhances butter flavor characteristics.The strategy provides an efficient method for producing flavor-enhanced butter with high industrial applicability.
  • SHENG Yanan, CHAO Jin, WU Haoren, YUAN Yong, LIU Xinguo, LI Xiaohua, YIN Feiyan, SHI Meng, WU Yuanjie
    Food and Fermentation Industries. 2026, 52(11): 152-160. https://doi.org/10.13995/j.cnki.11-1802/ts.044176
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    To explore the inhibitory effect of solid-state fermentation of Astragali radix by Eurotium cristatum on xanthine oxidase (XOD) activity and develop new uric acid-lowering products, this study isolates and purifies E.cristatum AH10 from Fuzhuan tea.Using XOD inhibition rate as the index, it optimizes the solid-state fermentation process through single-factor experiments and L9(34) orthogonal tests.It determines the total polysaccharide content in aqueous extracts of Astragali radix before and after fermentation, analyzes the total flavonoid content and antioxidant activities (DPPH and ABTS scavenging rates) in ethanol extracts, and conducts correlation analysis.Results show that the optimal fermentation conditions are 8 days of fermentation, 40% water content, and 3% inoculum size.Under these conditions, the XOD inhibition rate of the ethanol extract from fermented Astragali radix reaches [(79.13±4.39)%], significantly higher than that of the positive drug group with 50 μg/mL allopurinol [(63.81±1.89)%].After fermentation, the total flavonoid content in Astragali radix increases from (3.16±0.08) mg/g to (8.06±0.16) mg/g, while the total polysaccharide content decreases from (250.19±16.50) mg/g to (125.56±7.33) mg/g.The maximum DPPH and ABTS scavenging rates of fermented Astragali radix are (86.82±0.52)% (1.6 mg/mL) and (87.84±6.15)% (2.0 mg/mL), respectively, both significantly higher than those of the unfermented group at the same concentration [(35.73±2.20)% and (41.48±2.18)%].Correlation analysis indicates that XOD inhibition rate, DPPH scavenging rate, and ABTS scavenging rate are positively correlated with total flavonoid content and negatively correlated with total polysaccharide content.Fermentation by E.cristatum significantly enhances the XOD inhibitory capacity of Astragali radix by increasing total flavonoid content, providing a theoretical basis for the development of uric acid-lowering products.
  • GAO Jinyang, WU Shiru, CHENG Yin, WANG Boyang, LIU Chunlei, LI Dan
    Food and Fermentation Industries. 2026, 52(11): 161-169. https://doi.org/10.13995/j.cnki.11-1802/ts.045557
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    Silica gel column chromatography was adopted for the isolation and purification of extracts from the pileus of Dictyophora indusiata.Based on the antibacterial activity against Shewanella putrefaciens, the optimal active fraction was screened out, and liquid chromatography-mass spectrometry identified 2-methylbenzyl alcohol as the component with the highest relative content.The antimicrobial activity of the extract was systematically evaluated via indicators including inhibition zone diameter, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC).To thoroughly explore its antimicrobial mechanism, cellular morphology, intracellular fluorescence intensity, electrical conductivity, ultramicroscopic Na+-K+-ATPase activity, and bacterial growth kinetics were determined.Results demonstrated that the purified fraction from Dictyophora indusiata pileus exerted a significant antibacterial effect against S.putrefaciens (P<0.05), with a maximum inhibition zone diameter of (24.28±0.49) mm and MIC and MBC values of 0.781 3 and 6.25 mg/mL, respectively.The extract exerts its antibacterial effect by disrupting the integrity of S.putrefaciens cell membranes, increasing membrane permeability to induce intracellular substance leakage, and inhibiting Na+-K+-ATPase activity—thereby interfering with the bacterium’s metabolic processes.These findings provide theoretical support for developing Dictyophora indusiata pileus extracts as natural food preservatives and open new avenues for the high-value utilization of agricultural byproducts.
  • NIE Xiaozhong, XU Zhiqiang, LIU Wei
    Food and Fermentation Industries. 2026, 52(11): 170-177. https://doi.org/10.13995/j.cnki.11-1802/ts.045075
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    Astaxanthin, a lipophilic carotenoid, exhibits excellent antioxidant and anti-inflammatory bioactivity.However, its application in foods is largely limited due to poor water solubility, susceptibility to oxidative degradation, and low bioaccessibility.In this study, a ternary complex consisting of sodium caseinate (NaCas), γ-cyclodextrin (γ-CD), and dihydromyricetin (DMY) was prepared using a pH-driven method, and further employed as an emulsifier to construct an interfacial antioxidant astaxanthin-loaded emulsions.The effects of DMY concentration on the physicochemical properties, astaxanthin loading capacity, bioaccessibility, and stability of the emulsion were systematically investigated.The results indicated that although the astaxanthin encapsulation efficiency slightly decreased with increasing DMY content, it remained above 87.45%, while the interfacial protein adsorption showed an initial increase followed by a decline.During storage, the retention of astaxanthin consistently improved with higher DMY addition, reaching a maximum of (87.27±1.80)% (0.1% DMY, 40 ℃, 28 days).In vitro digestion simulation demonstrated that DMY facilitated the hydrolysis of triglycerides into free fatty acids (FFA), significantly enhancing the bioaccessibility of astaxanthin to (36.02±2.36)%.This study offers a promising strategy for the efficient encapsulation and delivery of easily degradable lipophilic bioactive compounds, and provides a theoretical foundation for developing functional emulsion products with high interfacial antioxidant capacity and improved bioaccessibility.
  • DENG Chunli, CHEN Weiling, HUANG Shuangquan, NIE Hui, LUO Yanghe
    Food and Fermentation Industries. 2026, 52(11): 178-185. https://doi.org/10.13995/j.cnki.11-1802/ts.044234
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    In this study, water chestnut starch octenylsuccinate with different degrees of substitution was prepared by aqueous phase method, using water chestnut starch was used as raw material and octenyl succinate anhydride (OSA) as esterification.The emulsifying property, anticoagulation and viscosity of OSA water chestnut starch with different degree of substitution were analyzed.The structure of starch esters was characterized by fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and scanning electron microscopy (SEM).The results showed that the degree of substitution of OSA water chestnut starch octenylsuccinate increased with the increase of OSA dosage, and the emulsifying power increased with the increase of substitution degree, while the anticoagulation showed a trend of first increasing and then decreasing with the increase of the degree of substitution.The peak viscosity, hold viscosity, final viscosity, and break down value of esterified starch were higher than that of the original starch.The FTIR results showed that a new characteristic peak was formed at 1 722 cm-1 after esterification, and the peak gradually increased with the increase of substitution.XRD results indicated that the crystallization of water chestnut starch was still C-type, the relative crystallinity decreased with the increase of degree of substitution.Through the observation of SEM, it was found that after the esterification treatment, the particle size of water chestnut starch increase, and the depression on the particle surface became more obvious with the increase of substitution.
  • CHEN Jinfeng, HOU Furong, WANG Wenliang, CUI Wenjia, JIA Fengjuan, SONG Shasha, GONG Zhiqing, SHI Junyan, JIA Chengli, ZHANG Jian, CHEN Jingxin, WANG Yansheng
    Food and Fermentation Industries. 2026, 52(11): 186-195. https://doi.org/10.13995/j.cnki.11-1802/ts.043153
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    The complex of sodium hyaluronate (SH) and whey protein (WP) was used as the external aqueous phase W2.A water-in-oil-in-water (W1/O/W2) double emulsion co-delivery system loaded with lipophilic dihydroquercetin (DHQ) and hydrophilic (-)-epigallocatechin gallate (EGCG) was constructed by single factor test and central composite design response surface method.Taking the concentration of polyglyceryl-6 polyricinoleate (PGPR) and the volume ratio of water to oil as the influencing factors, the optimum ratio of W1/O colostrum was screened by emulsion surface, microstructure and particle size:the concentration of PGPR was 4% (v/v), and the volume ratio of water to oil was 20% (v/v).Through single factor experiments, the mass ratio of W2 (SH-WP) in the external aqueous phase, the volume ratio of colostrum, the dispersion speed and time were screened.Based on this, the star point design effect surface method (CCD-RSM) was used to investigate the effects of W2 mass ratio and colostrum volume ratio on the particle size and Zeta potential of W1/O/W2 emulsions.The binomial and multiple linear regression models were used to fit and establish the relationship between indicators and factors, and the optimal process parameters were determined:SH-WP mass ratio of 3∶1, colostrum volume ratio of 63.32%, dispersion speed of 12 000 r/min, and dispersion time of 3 min.The predicted values of various indicators in the optimized process were close to the target values.The prepared emulsion has a white appearance and a uniform and stable system.The confocal laser scanning microscope (CLSM) staining image shows that the emulsion is a typical “two-film three-phase” interface structure.The structure of SH-WP complex was investigated by Fourier transform infrared spectroscopy, circular dichroism and fluorescence spectroscopy.The results showed that SH and WP were mainly electrostatic adsorption and hydrogen bond interaction.The three-phase contact angle investigation showed that the surface wettability of the SH-WP composite was significantly improved.The embedding rates of EGCG and DHQ in the prepared double emulsion were 41.24% and 95.34%, respectively, and the double emulsion had good stability.In summary, the W1/O/W2 double emulsion prepared in this study can simultaneously achieve the co-delivery of hydrophilic EGCG and lipophilic DHQ, and has good potential for active ingredient delivery.
  • LONG Tingting, LUO Yimeng, PENG Qinyan, WANG Han, XU Shijun, YE Jinsong
    Food and Fermentation Industries. 2026, 52(11): 196-204. https://doi.org/10.13995/j.cnki.11-1802/ts.044340
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    Aiming to improve the emulsifying performance of egg yolk liquid, this study explored the effects of ultrasonication pre-treatment combined with protein-glutaminase (PGase) and arginine (Arg) modification.The impact of different modification conditions was systematically evaluated.We characterized changes in average particle size, zeta-potential, surface hydrophobicity, free sulfhydryl group content, SDS-PAGE, Fourier transform infrared spectroscopy, intrinsic fluorescence spectroscopy, ultraviolet-visible absorption spectroscopy, and scanning electron microscopy.These analyses revealed the underlying mechanisms.The results demonstrated a strong synergistic effect between ultrasonication, enzymatic deamidation by PGase, and chemical modification by Arg.This combination effectively altered protein structural characteristics by inducing protein unfolding, exposing hydrophobic groups, enhancing intermolecular electrostatic repulsion, thickening the adsorbed layer at the oil-water interface, and accelerating the migration rate of proteins to the interface.Consequently, emulsion stability improved.Specifically, the sequential treatment involving ultrasound pre-treatment assisted PGase modification followed by Arg addition yielded the most significant enhancement.Emulsifying activity increased by 185.59% and emulsifying stability by 249.42% compared to the control group.This group also exhibited the smallest average particle size, the highest absolute zeta-potential value, significantly increased surface hydrophobicity and free sulfhydryl group content, and distinct alterations in protein secondary and tertiary structures.In conclusion, the combination of ultrasound pre-treatment with PGase and Arg modification is an effective strategy for improving the emulsifying properties of egg yolk liquid.
  • XU Jinglei, CHEN Mengke, WEN Jiping, SHEN Yuxiang, AO Tianfang
    Food and Fermentation Industries. 2026, 52(11): 205-211. https://doi.org/10.13995/j.cnki.11-1802/ts.044002
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    This study used cassava starch as the raw material and modified it through wet heat treatment with different moisture contents (15%, 20%, 25%, and 30%).The effects of wet heat treatment on the gel properties and 3D printing performance of cassava starch were investigated.The results showed that after wet heat treatment, the storage modulus (G′) and loss modulus (G″) of cassava starch gels increased, while tanδ decreased.The gel strength of the starch was enhanced, and all samples exhibited gel characteristics dominated by elasticity, improving the stability of the starch gel and facilitating the maintenance of the shape of 3D printed products.The viscosity, retrogradation value, hardness, and chewiness of the starch gel system decreased, with the viscosity dropping by up to 419 mPa·s and the retrogradation value by up to 454 mPa·s.The binding ability of starch molecules with hydroxyl groups increased, and the water-holding capacity of the starch improved.The pore size decreased, and the porous network structure became denser and more uniform, enhancing the ability of the starch gel to maintain its shape.When the moisture content for wet heat treatment was 20%, the G′ and G″ of the samples increased, the surface texture of the printed samples was clear, and the printing error was the smallest, resulting in the best printing effect of the gel system.
  • HUA Lei, LI Jinjin, WANG Jingxing, ZHAO Xiangru
    Food and Fermentation Industries. 2026, 52(11): 212-222. https://doi.org/10.13995/j.cnki.11-1802/ts.045352
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    To investigate the inhibitory effect of edible flowers on the formation of harmful substances in biscuits, this study incorporated floral powder as a food component into the biscuit formulation.Analysis was conducted on the basic nutritional components, polyphenolic composition, and changes in polyphenols before and after baking, alongside the correlation between polyphenols and harmful substance inhibition rates.This aimed to identify the key bioactive compounds in edible flowers responsible for inhibiting harmful substance formation, with validation through single-component addition experiments.Results indicated that among seven edible flowers, Osmanthus fragrans and Sophora japonica significantly inhibited acrylamide, 5-hydroxymethylfurfural, and advanced glycation end products by enhancing the antioxidant capacity of biscuits.Polyphenolic composition analysis revealed O. fragrans was rich in verbascoside. Following baking, all polyphenol contents in biscuits decreased significantly (P<0.05).Among these, cinnamic acid, p-hydroxybenzoic acid, and verbascoside showed strong correlations with harmful substance inhibition rates.The addition of verbascoside significantly inhibited the formation of harmful substances in the control biscuits, the negative control (S. japonica biscuits), and the positive control (rose biscuits).In summary, incorporating O. fragrans flower powder or verbascoside (1.54 mg/100 g wheat flour) into baked biscuits effectively inhibits the formation of heat-processed hazards, providing technical support and theoretical guidance for developing nutritious and healthy baked foods.
  • SHEN Yuxiang, WEN Jiping, ZHANG Zhaoyang, CHEN Mengke, GAO Qingyang, AO Tianfang, XU Jinglei
    Food and Fermentation Industries. 2026, 52(11): 223-232. https://doi.org/10.13995/j.cnki.11-1802/ts.043810
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    In the context of the vigorous development of food 3D printing technology, to improve the accuracy and storage stability of wheat starch gel 3D printing products, this study explored the effects of different germination time (0, 12, 24, 36, and 48 h) on the 3D printing characteristics of wheat starch, by using wheat starch as the raw material, and the applicability of 3D printing of wheat starch was optimized.It investigates the relationships between the 3D printing characteristics of germinated wheat starch and the gelatinization properties, rheological properties, texture, and microstructure of starch gels.Results showed that all samples could present complete shape contours in 3D printing, but there were significant (P<0.05) differences in details.At 36 h of germination, the pasting viscosity of wheat starch was moderate, and the rheological properties showed good synergy between storage modulus (G′) and loss modulus (G″), suitable cohesion and adhesiveness, and high crystallinity.The printed “Superman logo” and “Gear cylinder” samples had the smallest size deviation, the accuracy was 0.43% and 0.87%, and the stability (2.53% and 1.21%) was the best.In conclusion, appropriate germination treatment of wheat could improve the 3D printing characteristics of its starch gel, providing a theoretical basis and process reference for the development of 3D printed food materials.
  • WU Wanyi, WU Hongying, CAO Wenhong, LIN Haisheng, GAO Jialong, FAN Xiuping, ZHENG Huina, QIN Xiaoming
    Food and Fermentation Industries. 2026, 52(11): 233-242. https://doi.org/10.13995/j.cnki.11-1802/ts.044427
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    Understanding the structure-activity relationship and stability of marine shellfish-derived immunomodulatory peptides is crucial for the development of related functional foods.This study used molecular docking and kinetic simulations to identify potential immunoregulatory targets among 16 peptides with known sequences.Cellular experiments validated their activity, and the stability of the most promising peptide was evaluated.Results showed that QLNWD exhibited the strongest binding affinity to toll-like receptors2 (TLR2) and toll-like receptors4/myeloid differentiation factor 2 (TLR4/MD-2), with binding energies of -8.3 kcal/mol and -8.1 kcal/mol, respectively.Molecular dynamics simulations further revealed a more stable and compact conformation when QLNWD was bound to TLR4/MD-2.Among the four candidate peptides (QLNWD, RVAPEEHPVECRYLV, LLQLGSGR, and DNSIAMESMK), QLNWD enhanced phagocytic activity and nitric oxide release in RAW264.7 cells in a dose-dependent manner, indicating superior immunostimulatory potential.Stability analysis showed that QLNWD had a gastrointestinal digestion retention rate of only (10.48±0.39)%, and its immunological activity was sensitive to environmental stressors such as high temperature, strong alkaline conditions, and metal ions.These findings confirm the potential of QLNWD as a natural immunomodulator and provide a theoretical foundation for the rational screening of marine-derived bioactive peptides and the development of functional foods.
  • LYU Siying, BAO Jianqiang, JIN Yinzhe, LI Xueyan, CHENG Qianyi
    Food and Fermentation Industries. 2026, 52(11): 243-251. https://doi.org/10.13995/j.cnki.11-1802/ts.044497
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    In order to enhance the utilization value of a new species of conch Cymbium pepo, its physicochemical properties were analyzed.In particular, the effects of pepsin, trypsin, alcalase, neutrase, and flavourzyme on the physicochemical, structural, and functional properties of the C. pepo hydrolysates were investigated.Physicochemical properties were determined by degree of hydrolysis and peptide content, structural changes were characterized by UV spectra and CD spectra, and functional properties were investigated by solubility and emulsification.The experiments proved that C. pepo is a kind of conch with high protein and low fat, rich in various minerals. Hydrolysates prepared by different proteases featured a rich variety of amino acids, high degree of hydrolysis and peptide content, as well as smaller molecular weight and particle size. Moreover, enzymatic hydrolysis not only disrupted the secondary and tertiary structures, but also improved the functional properties of the hydrolysates. Especially, hydrolysates prepared by alcalase had a high degree of hydrolysis and peptide content of 23.10% and 5.505 mg/mL, respectively, and the molecular weight below 10 kDa, while the hydrolysis capacity of pepsin was relatively weak and differed significantly from that of other proteases.In conclusion, these findings can positively promote the further processing of C. pepo and provide a theoretical basis for the research and development of hydrolysates in C. pepo.
  • YUE Fengli, HUANG Zhongmin, CUI Xudong, LEI Mengmeng, FAN Wen, WANG Jiali, FU Saiya, PAN Zhili
    Food and Fermentation Industries. 2026, 52(11): 252-260. https://doi.org/10.13995/j.cnki.11-1802/ts.044546
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    This paper studies the effects of four different freezing methods, namely conventional freezing, low-temperature freezing, ultra-low-temperature freezing and liquid nitrogen immersion freezing, on the physicochemical quality, microstructure and sensory evaluation of braised Yellow River carp.The results showed that the time for generating the maximum ice crystal formation zone through the four freezing methods was 100, 35, 20 and 0.5 min respectively.The freezing rate of the LNF group was the fastest, the color was better, and the hardness, elasticity and chewiness were significantly higher than those of the other treatment groups (P<0.05), the thawing loss was the lowest, and the proportion of free water P22 was lower.The results of scanning electron microscopy showed that the LNF group had a relatively compact microstructure, with smaller myofibrillary gaps and more uniform dispersion.The analysis of electronic nose and electronic tongue revealed that the freezing method had a different effect on the flavor of braised Yellow River carp.The LNF group was closer to the fresh sample and had the highest sensory score, demonstrating higher acceptability.Overall comparison shows that LNF treatment can more effectively maintain the quality of braised Yellow River carp, which can provide certain theoretical support for the storage and preservation of pre-prepared dishes of braised Yellow River carp.
  • ZHENG Zengtuo, LIU Xinqi, TUYATSETSEG JAMBAL, YI Rule, DA Lichao, LIANG Jianzhong, LI Yuqi, JI Rimutu, MENGHE Bilige, MING Liang
    Food and Fermentation Industries. 2026, 52(11): 261-270. https://doi.org/10.13995/j.cnki.11-1802/ts.042636
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    This study focused on camel meat myofibrillar protein (MP), investigating a series of indicators across different pH value systems, including solubility, hydrophobicity, thiol content, carbonyl content, myofibrillar fragmentation index, particle size, zeta potential, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), gel color and whiteness, water-holding capacity, and microstructure.The objective was to explore the impact of various pH value buffer systems on camel MP and its thermally induced gels.Results indicated that as pH value increased, the solubility of MP initially increased then decreased, reaching its maximum at pH 6.5;hydrophobicity gradually decreased;total and reactive thiol contents both showed a decreasing trend;carbonyl content peaked at pH 6.0.Emulsification activity index was highest at pH 5.5, while emulsion stability index was highest at pH 6.5.The absolute value of the zeta potential reached its maximum at pH 6.5, whereas particle size was largest at pH 5.5, with particle size distribution becoming smaller overall as pH value increased.SDS-PAGE results revealed differences in the clarity and molecular weight distribution of protein bands under different pH value conditions.Gel brightness (L*) and whiteness increased with increasing pH, while a* and b* values decreased with rising pH value, and water-holding capacity was optimal at pH 6.5.Microstructural observations showed that at pH 6.5, the gel structure was the most compact and uniform.These findings suggest that pH value significantly affects the functional and gel-forming properties of camel MP, with pH 6.5 potentially being the optimal pH value for both MP and its gel formation.This study provides theoretical insights into the processing and research of camel meat products.
  • LI Jingyun, TANG Shanhu, LI Sining, MIAO Hao, XU Yingping
    Food and Fermentation Industries. 2026, 52(11): 271-277. https://doi.org/10.13995/j.cnki.11-1802/ts.043808
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    Muscle proteins are particularly susceptible to oxidation induced by reactive oxygen species generated during the processing and storage of meat products.Therefore, this study systematically investigated the influence mechanism of different taxifolin (Tax) mass fraction (0%, 0.2%, 0.8%, and 1.5%) on the oxidation degree and structural characteristics of myofibrillar protein (MP) in yak meat under the Fenton oxidation system.The results showed that introducing Tax into the yak meat system could significantly inhibit hydroxyl radical-induced carbonylation reactions.When the Tax mass fraction was within the range of 0-0.8%, it not only increased the content of active thiol groups and free amino groups in MP, but also effectively reduced system turbidity.Notably, when the Tax mass fraction reached 1.5%, a negative regulatory effect was observed, manifested as aggravated protein oxidative damage, significantly increased MP aggregation, and a marked decline in system stability.SDS-PAGE electrophoresis analysis further revealed that Tax mass fraction of 0-0.8% provided significant protection for myosin heavy chain and actin, whereas the high mass fraction of 1.5% Tax led to reduced myosin heavy chain band intensity.In summary, the findings confirm that a Tax mass fraction of 0.8% can effectively delay the oxidative damage process of MP and demonstrate promise for practical applications.
  • XU Jinchao, ZHAO Xiaolong, LI Jing, ZHAO Jie, CHEN Hongyu, BAI Ma, LI Liang, LIU Zhendong
    Food and Fermentation Industries. 2026, 52(11): 278-287. https://doi.org/10.13995/j.cnki.11-1802/ts.044331
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    To investigate the effects of different ingredient combinations on the quality of stewed yak meat, this study stewed yak meat with potatoes, radishes, and tomatoes, respectively.The nutritional, physicochemical, textural, and flavor characteristics of yak meat after different ingredient combinations were measured.The results revealed certain differences in the quality of stewed yak meat with different ingredients:the highest protein content was observed in plain stewed yak meat [(37.95±0.63)%].The highest fat content was found in plain-stewed yak meat [(1.23±0.10)%], significantly higher than the other groups (P<0.05).Potato-stewed yak meat exhibited the highest lightness (L*), while tomato-stewed yak meat had the highest redness (a*), and plain-stewed yak meat had the highest yellowness (b*).Tomato-stewed yak meat had the highest acidity (pH 5.94±0.02).The steaming loss of plain stewed yak meat was the highest [(49.95±0.15)%], significantly higher than the other groups (P<0.05).Potato- and tomato-stewed yak meat had lower hardness and better springiness.Scanning electron microscopy analysis revealed that the muscle fiber structure of potato-stewed yak meat was relatively loose, while that of tomato- and radish-stewed yak meat was relatively dense.GC-MS analysis revealed a total of 38 compounds, with aldehydes being the most abundant.The ingredient combinations influenced the release of aromatic and acidic substances.Potato-stewed yak meat performed best in terms of texture and the release of aromatic substances, while tomato- and radish-stewed yak meat excelled in color difference and pH, as well as the release of acidic and alcohol substances, respectively.This study provides theoretical support for the selection of ingredient combinations for stewed yak meat.
  • XU Yao, HUANG Jiaqi, FAN Xiuping, QIN Xiaoming
    Food and Fermentation Industries. 2026, 52(11): 288-298. https://doi.org/10.13995/j.cnki.11-1802/ts.044325
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    To investigate the quality and flavor changes of oyster sauce under different storage temperatures after opening, this study employed flat-plate simulations to monitor three oyster sauce samples over an 80-day period at 25 ℃ and 4 ℃ following opening.Timed sampling was conducted to analyze sensory changes, total mold counts, quality indicators, and flavor compounds in order to determine the optimal storage temperature.Results indicate that at 25 ℃, three oyster sauces exhibited significant sensory changes by day 15 post-opening, accompanied by marked increases in total mold count, moisture content, amino nitrogen, volatile basic nitrogen, and total acidity (P<0.05), while protein content decreased significantly (P<0.05) and viscosity also declined.Electronic nose analysis revealed notable flavor differences between the stored and original samples.Electronic tongue analysis and free amino acid measurements indicated a reduction in umami flavor in the stored samples.In contrast, all samples stored at 4 ℃ showed no significant changes in sensory characteristics, total mold count, or physicochemical parameters throughout the storage period.Analysis using the electronic nose, electronic tongue, and free amino acid assays indicated a reduction in flavor compounds over time.Comprehensive analysis revealed that the flavor of oyster sauce diminished at both storage temperatures;however, storage at 4 ℃ was more effective in maintaining overall product quality compared to 25 ℃.
  • TIAN Feifan, XU Mingqiang, ZHANG Jian, MIAO Ruiyin, WANG Ruizhu, CHEN Kai
    Food and Fermentation Industries. 2026, 52(11): 299-309. https://doi.org/10.13995/j.cnki.11-1802/ts.044004
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    To investigate the effects of plasma treatment time and heat pump drying temperature on the drying characteristics and quality of jujube slices, this study employed atmospheric pressure plasma jet (APPJ) to pretreat jujube slices (0, 2, 5, and 8 s), followed by heat pump drying at different temperatures (40, 50, and 60 ℃).The effects of different plasma treatment times and drying temperatures on the drying characteristics, small-molecule sugar content, total phenols, total flavonoids, texture properties, and color of jujube slices were analyzed.A fuzzy mathematical sensory evaluation model was established to validate their acceptability.The results showed that heat pump drying after plasma treatment required less time.The APPJ5s-60 ℃ group exhibited the greatest reduction in drying time compared to APPJ0s-60 ℃ (20% decrease).Scanning electron microscope observations revealed that plasma treatment etched the surface of jujube slices, creating larger pores and forming new moisture transport channels, which facilitated water loss and directly improved the drying rate.By fitting four commonly used empirical drying models (Page, Logarithmic, Verma model, and Wang and Singh), it was found that the Page model accurately described the moisture change of jujube slices dried at 40 ℃ and 60 ℃, while the Logarithmic model provided a better fit for those dried at 50 ℃.Plasma treatment also influenced the content of small-molecule sugars, total phenols, and total flavonoids in jujube slices.Compared with APPJ0s-50 ℃, the APPJ5s-50 ℃ group showed a 1.73% increase in total small-molecule sugar content, an 11.92% increase in total phenols, and a 4.24% increase in total flavonoids.Additionally, plasma treatment created a complex pore structure in the jujube slices, enhancing their crispness and chewiness, thereby improving their texture properties.Based on the constructed fuzzy sensory mathematical model, the comprehensive evaluation scores of plasma-treated jujube slices increased, with the APPJ5s-50 ℃ group scoring the highest at 91.96.In conclusion, plasma treatment can alter the surface structure of jujube slices, accelerating the drying process and directly reducing thermal reaction time during drying, thereby improving drying efficiency and the retention of nutritional quality.The APPJ5s-50 ℃ group preserved the original quality of jujube slices and achieved the highest fuzzy mathematical sensory evaluation score, making it more acceptable to consumers.
  • ZHUANG Weiran, ZHU Xiaoyi, ZHANG Yumei, YANG Yi, FANG Weirui, WU Jing, WU Qiumei, LIU Kunlin, YI Chengjing, LIU Yaowen
    Food and Fermentation Industries. 2026, 52(11): 310-321. https://doi.org/10.13995/j.cnki.11-1802/ts.044292
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    Long’an pomelos are susceptible to pathogen infection after harvest, and traditional chemical agents are not effective in preserving them.In order to extend the storage life of Long’an pomelos, this experiment loaded Bacillus subtilis into edible coatings of different concentrations and substances to determine their performance, screened out the most suitable coating solution, and applied it to the storage and preservation of Long’an pomelos.The results shows that 2.0% sodium alginate (SA) is the most suitable coating for B.subtilis, and the optimal antibacterial concentration of B.subtilis is 1×108 CFU/mL.The B. subtilis/SA coating solution effectively reduces water vapor permeability to (3.22±0.19) g·mm/(m2·d·kPa) and increases elongation at break to (3.56±0.23)%.Furthermore, the coating demonstrates excellent preservation properties.At day 90, the B.subtilis/SA coating-treated group exhibits the highest hardness (18.62±0.55) N and the lowest weight loss rate (7.6±0.91)%, with the smallest decreases in soluble solids and titratable acid content, decreasing by (2.25±0.3)%, (0.17±0.01) mg/g, respectively, indicating that the coating can slow down the deterioration of the fruit.The Long’an pomelo treated with B.subtilis/SA coating shows a slow decline in flavonoids and lycopene during storage, reaching (3.07±0.16) OD325/g FW and (0.11±0.01) OD502/g FW, respectively.with small increases in malondialdehyde (21.37±2.48) mmol/g and lignin (0.16±0.02) OD502/g FW, thereby enhancing the antioxidant capacity of Long’an pomelos and extending their post-harvest storage period from 30 days to 90 days.In summary, the B.subtilis/SA edible coating solution prepared in this study ensures the post-harvest storage and preservation quality of Long’an pomelos, providing a reference for improving the preservation effect of Long’an pomelos.
  • SU Tingting, WANG Xueqing, DING Zhenzhen, FENG Yan, WANG Yutao, YU Yigang
    Food and Fermentation Industries. 2026, 52(11): 322-328. https://doi.org/10.13995/j.cnki.11-1802/ts.044136
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    To investigate the fruit quality, nutrient composition and their in vitro antioxidant activities of different pomegranate varieties, four varieties of pomegranates fruit from Southern Xinjiang (soft-seeded, Piyaman, Caohu, Baixiang) were used for the determination of the single fruit weight, longitudinal/transversal diameters, chromaticity, soluble solids, titratable acidity, total phenolics, total flavonoids, anthocyanins, and their antioxidant activities [DPPH radical, ABTS radical cation scavenging capacity and ferric ion reducing antioxidant power (FRAP)].The results showed that the soft-seeded pomegranate exhibited the highest single fruit weight (444.46 g) and fruit shape index (0.93).Its color parameters L* (61.09) and b* (28.12) were significantly higher than those of other varieties, indicating a bright appearance with a distinct yellow hue.The Piyaman pomegranate exhibited the highest a* (39.44), representing the best red saturation.The Caohu pomegranate had the highest soluble solids content, while the Piyaman pomegranate has the highest titratable acidity.Notably, total phenolics (6.44 mg GAE/g) and total flavonoids (2.58 mg RE/g) were the highest in Caohu, while anthocyanins was the highest in Piyaman at 0.093 mg/g.In addition, all four pomegranate varieties demonstrated strong in vitro antioxidant activity.Among them, the Caohu pomegranate showed significantly superior ABTS radical cation scavenging capacity (88.33%) and FRAP (6.56 μmol Trolox/g), which may be attributed to its higher levels of total phenolics, total flavonoids and anthocyanins.This study provides a theoretical basis for the quality evaluation and development of high-value functional products of pomegranate resources in Southern Xinjiang.
  • JIN Zixia, ZHOU Zhilei, MENG Nan, SUN Xiaotao, XIONG Yanfei, AO Ling, REN Qingxi, JI Zhongwei, MAO Jian
    Food and Fermentation Industries. 2026, 52(11): 329-338. https://doi.org/10.13995/j.cnki.11-1802/ts.044562
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    Retronasal perception better reflects the aromatic experience during the entire consumption of Baijiu.However, the detection of retronasal volatile compounds remains a challenge in the industry due to their low concentration and dynamic changes.A method was developed to detect dynamic retronasal volatile compounds in Baijiu.This was achieved by collecting a single nasal exhalation with a gas sampling bag, followed by direct injection into gas chromatography-ion mobility spectrometry (GC-IMS).The total absolute peak volumes (APVs) of compounds measured after swallowing were significantly higher than those after spitting.The total APVs increased with larger sip volumes but showed no significant change beyond 4 mL.With extended sip duration, the total APVs first increased and then decreased, peaking at 10 seconds.Using this method, the composition and dynamic release behavior of retronasal aroma compounds in four strong-aroma Baijiu from two representative regions were investigated.A total of 32, 34, 29, and 28 compounds were identified in the four Baijiu samples, primarily esters and alcohols.Based on their release behavior, the compounds were categorized into Group I (delayed release), Group II (slow release), and Group III (rapid release).Most esters were rapidly released, while longer carbon chains in alcohols correlated with faster release.The release of retronasal volatile compounds in strong-aroma Baijiu results from the interplay of their physicochemical properties, the complex matrix of Baijiu, and the oral environment.This study provides a theoretical basis for understanding the dynamic changes in flavor during the consumption of strong-aroma Baijiu.
  • XU Shiyong, ZHANG Shizhi, SHI Ting, ZHANG Mingjin
    Food and Fermentation Industries. 2026, 52(11): 339-345. https://doi.org/10.13995/j.cnki.11-1802/ts.044417
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    In response to the phenomenon of adulteration in the market of liquor, the search for rapid, non-destructive and scientific methods for identifying the quality of liquor has become one of the research hotspots in the relevant fields.This study aims to quickly identify the “Chinese Huzhu Qingke Liquor” (CHQL), a product protected by geographical indication in our country.Three types of liquor samples, namely CHQL, other brand Qingke Liquor (OBQL), and non-Qingke based Baijiu (NQBB), were collected.The spectral characteristics of these three types of liquor were studied using UV-near-infrared fusion spectroscopy.An attempt was made to establish a rapid identification method for CHQL.Firstly, the UV spectra and near-infrared spectra of the samples were collected, and the spectra were fused at the data level.Then, four preprocessing methods (standard normal transformation, Savitzky-Golay smoothing, first-order derivative, and second-order derivative) and three classification models (BiGRU, Bayes-BiGRU, Bayes-Transformer-BiGRU) were respectively examined for the identification effect of CHQL.The results show that the data obtained after second-order derivative preprocessing combined with Bayes-Transformer-BiGRU has the best classification recognition effect, with classification accuracy of 94.12%, response time of 492.89 seconds, and PAM of 0.87.This indicates that Bayes-Transformer-BiGRU combined with UV-NIR fusion spectroscopy can quickly, non-destructively and accurately distinguish CHQL.
  • LI Aimeng, WU Dong, YANG Jian, ZHOU Zonghui, QIU Zhiqiang, LI Jinzhuang, JIA Lirong
    Food and Fermentation Industries. 2026, 52(11): 346-357. https://doi.org/10.13995/j.cnki.11-1802/ts.044311
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    To investigate the differences in non-volatile metabolites between fermented and non-fermented hot pot bases, hot pot bases were prepared using Ciba chili paste and fermented Ciba chili paste as base materials.LC-MS/MS metabolomics was used to analyze metabolic differences between the fermented base (oil phase YX-F, water phase SX-F) and the non-fermented base (oil phase YX, water phase SX).The results showed that a total of 1 162 non-volatile metabolites were identified across the four sample groups, covering 13 categories of components such as lipids, organic acids and carbohydrates.Overall, the content of lipids in fermented samples decreased significantly.Comparative analysis revealed that 124 differential metabolites were identified in YX-F and YX, with 202 detected in SX-F and SX.These metabolites were predominantly enriched in metabolic pathways including arachidonic acid metabolism, galactose metabolism, and the TCA cycle.Changes in differential metabolites showed that probiotic-related organic acids (e.g., succinic acid, 3-phenyllactic acid) and carbohydrates (e.g., galactitol, sorbitol) were significantly increased in YX-F and SX-F, while irritating compounds (e.g., piperine, dihydrocapsaicin) were significantly reduced.These changes synergistically maintain gastrointestinal homeostasis.The study confirmed that fermentation of Ciba chili paste raw material can effectively increase gastrointestinal health-promoting compounds and reduce undesirable components in hot pot base products, thereby helping to alleviate gastrointestinal discomfort caused by traditional hot pot diets.The experimental results provide a theoretical basis for developing “gastrointestinal-friendly” hot pot base products.
  • WANG Yunhai, MA Wenqiang, XIANG Binbin, CUI Kuanbo, YANG Liling, MA Wenjie, HE Meiling, ZHU Zhaoshuai, SUN Lina
    Food and Fermentation Industries. 2026, 52(11): 358-367. https://doi.org/10.13995/j.cnki.11-1802/ts.044343
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    In order to realize the qualitative discrimination and quantitative prediction of key quality indexes in the storage stage, the ‘France’ plum during the storage period was taken as the object in this study.The near-infrared spectra of plum during the storage period of 0, 15, 30, 45 and 60 days were collected.Combined with pretreatment methods such as normalization, convolution smoothing, multiple scattering correction and standard normal transformation, and feature extraction methods such as competitive adaptive reweighted sampling, uninformative variable elimination and genetic algorithm, the qualitative discrimination models of support vector machine, K nearest neighbor and linear discriminant analysis were constructed respectively.Single quality benchmark prediction model of soluble solids content, moisture content and hardness with partial least squares regression.On this basis, the interpretable machine learning SHapley Additive exPlanations(SHAP) method is introduced by combining the characterization wavelengths of soluble solids content and water content with the characterization wavelengths of fruit firmness, and it was applied to the contribution evaluation and phased screening of the fused characteristic wavelengths, and an optimized fruit firmness fusion prediction model is constructed.The results showed that the qualitative discriminant model of MSC-GA-LDA storage stage was 100% accurate on the test set (the sample size of the test set n=75, covering all storage stages).In the single quality benchmark prediction model, 30,54 and 15 key response characteristics were screened out from the soluble solids content, water content and fruit firmness models, respectively.The optimal performances of the corresponding models were SNV-CARS-PLSR (R2p=0.922 2, RMSEp=0.663 9%), SNV-GA-PLSR (R2p=0.809 2, RMSEp=1.806 e-02%) and SNV-CARS-PLSR (R2p=0.809 0, RMSEp=0.103 6 N).The fusion model screened by SHAP significantly improved the prediction effect of fruit hardness (R2p=0.848 5, RMSEp=0.097 5 N), which provided theoretical basis and technical support for the non-destructive detection of storage quality of plums.
  • CHEN Haolei, HUANG Di, RAO Qinxiong, LI Huaxi, CAO Xiaolong, DENG Zhongsheng, SONG Weiguo
    Food and Fermentation Industries. 2026, 52(11): 368-375. https://doi.org/10.13995/j.cnki.11-1802/ts.044174
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    Polybrominated dibenzo-p-dioxins and furans (PBDD/Fs) have a similar structure to polychlorinated dibenzo-p-dioxins and furans (PCDD/Fs), thus exhibiting similar toxic effects and bioaccumulation characteristics in organisms.They can pose risks to humans through dietary intake via aquatic products and other sources.To investigate the dietary exposure risks of PCDD/Fs and PBDD/Fs in aquatic products from the East China Sea to the human body, this study used HRGC-HRMS to detect 14 PBDD/Fs congeners and 17 PCDD/Fs congeners in 24 species of aquatic products from the East China Sea, and calculated their toxic equivalent quantity (TEQ).The aim was to understand their potential sources, exposure levels, and to conduct risk assessment.The results showed that the detection rate of PCDD/Fs and PBDD/Fs in all aquatic products was 100%.The TEQ of fish, mollusks, crustaceans, and algae samples was 0.02-0.48, 0.05-1.55, 0.05-0.41, and 0.02-0.08 pg WHO-TEQ/g on a wet weight basis, respectively.The concentration of PBDD/Fs in aquatic products was higher than that of PCDD/Fs.In the risk assessment, the intake, daily safe exposure dose, and carcinogenic risk for each age group were all within the safe range specified by the World Health Organization, and the hazard quotient for all population groups was much less than 1.Except for mollusks and algae, the TEQ of other aquatic species shows ΣPCDD/Fs>ΣPBDD/Fs.The daily exposure amounts in descending order were children, adolescents, and adults, and the main contributing source for these three population groups was mollusks.In conclusion, the TEQ of PCDD/Fs and PBDD/Fs in east China sea aquatic products was relatively low, and the exposure risk to eastern coastal residents was low, remaining at a safe level.
  • ZHAN Yuting, QIU Xiaoyu, LIN Qinbao, YANG Qinghua, LU Shiqiang, LI Nannan, MA Hongsheng
    Food and Fermentation Industries. 2026, 52(11): 376-382. https://doi.org/10.13995/j.cnki.11-1802/ts.043791
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    The migration risk of per- and polyfluoroalkyl substances (PFAS) from food packaging paper into food remains a concern.However, the detection methods for PFAS are complex and time-consuming, presenting ongoing challenges to regulatory control systems.This study preliminarily validated the relationship between 24 kinds of PFAS and the levels of total fluorine (TF) and extractable organic fluorine (EOF).Measurement of paper samples spiked with these 24 PFAS demonstrated that the EOF indicator could effectively reflect their content.The contents of TF, inorganic fluorine (IF), and EOF in 19 commercially available food packaging papers were determined using online combustion-ion chromatography.The results showed that, compared to other paper materials, oil-resistant paper products exhibited significantly higher fluorine levels, with TF and EOF ranging from 458.55 to 741.30 mg/kg and 10.28 to 61.04 mg/kg, respectively.This might be associated with the addition of fluorinated oil-resistant additives in these products.The findings of this study provide a reference for the regulatory control of PFAS in food packaging paper.
  • ZHANG Yao, HUI Qiaoni, GUO Ying, SONG Liya
    Food and Fermentation Industries. 2026, 52(11): 383-392. https://doi.org/10.13995/j.cnki.11-1802/ts.043638
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    Postbiotics, defined as non-viable microbial preparations containing functional components, comprise bioactive constituents such as organic acids, bacteriocins, and exopolysaccharides.Recently, postbiotics have emerged as a novel antimicrobial strategy to replace traditional probiotics, demonstrating significant application value in food preservation and infection control.Exhibiting high targeting specificity in host-microbe interactions, postbiotics demonstrate potent antimicrobial activity against diverse pathogens, while circumventing the risks inherent to live bacterial applications, along with enhanced manufacturing stability and storage tolerance.The antimicrobial efficacy of postbiotics primarily stems from their diverse active components and multifaceted mechanisms of action.This review summarized source strains of postbiotics and their main antibacterial substances.Classification covered low-molecular-weight metabolites, high-molecular-weight metabolites, and cellular components.Description included functional properties of organic acids, lipids, peptides, exopolysaccharides, and teichoic acids.The review then detailed several antibacterial mechanisms of postbiotics.This work will provide references for research on postbiotics as novel antibacterial substances, and support multi-angle development of antibacterial products.
  • LIU Wei, XI Xichun, WEN Xueqiu, CHEN Weibo, LIN Zeqian, ZENG Xiaofang, HUANG Guiying
    Food and Fermentation Industries. 2026, 52(11): 393-403. https://doi.org/10.13995/j.cnki.11-1802/ts.044240
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    As a special color, aroma, taste, and quality condiment, brew soy sauce was habited to flavour adding for Chinese people predisposition, and its history is of long standing and well established.The production processes of brew soy sauce were in sequence of raw material pretreatment, koji-making, fermentation, and oil extraction.Among there, the koji-making process was a key to the flavor and quality of brew soy sauce, because it determined the ecological structure of microbial flora, which affected the utilization rate of raw materials and the process of subsequent fermentation.At present, the quality improvement of chinese soy sauce has been hindered by a lack of systematic and comprehensive research on brewing raw materials, brewing strains and mixed strains, which limits its development toward high-end and international markets.The article pertinently discusses the aforementioned issues with Chinese brew soy sauce and systematically reviews the research progress of soy sauce koji-making technology in China from three aspects:optimizing koji-making materials, improving Aspergillus oryzae species, and mixed-strain koji-making (including double-strain and multi-strain koji-making).It also prospects the future development direction, aiming to enhance the domestic brewing soy sauce koji-making technology level and provide a strong reference for relevant theoretical research.
  • WANG Dachen, WANG Zijie, JIANG Yushan, JIANG Xuesong, JIANG Hongzhe, ZHOU Hongping, WANG Yingli
    Food and Fermentation Industries. 2026, 52(11): 404-415. https://doi.org/10.13995/j.cnki.11-1802/ts.044173
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    With the enhancement of consumers’ awareness of food quality and safety, nondestructive testing of meat quality has become an inevitable demand for the development of the modern meat industry.Spectroscopy and imaging technologies can rapidly and accurately assess the sensory characteristics, physicochemical properties, and hygiene and safety indicators of meat.This paper reviews the application of spectroscopy and imaging technologies in meat quality inspection, mainly introducing the theoretical basis, development status, current applications, advantages, and disadvantages of these technologies, and looks forward to the future development trends.Although these technologies have achieved remarkable results in laboratory environments, they still face challenges, including high costs, numerous interference factors in complex environments, and complex data analysis processes in industrial applications.In the future, multiple low-cost techniques can be integrated and combined with deep learning algorithms to improve the comprehensiveness and accuracy of detection, promoting their practical application in the meat industry.
  • LIU Jiaxuan, GAO Jiabin, WU Wenjin, CHEN Sheng, SHI Liu, CHU Shang, GUO Xiaojia, LIN Jiahao, WANG Lan, QIAO Yu, XIONG Guangquan, LIU Bin, CHEN Lang
    Food and Fermentation Industries. 2026, 52(11): 416-427. https://doi.org/10.13995/j.cnki.11-1802/ts.043999
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    Body weight stands as a pivotal measurable attribute of fish, recognized as a decisive factor in standardized size grading systems, precision processing parameterization frameworks, and market value evaluation mechanisms.The rapid advancement of computer hardware and intelligent optimization algorithms demonstrates the potential to drive revolutionary progress in non-destructive weight detection technologies based on machine vision, establishing these systems as robust tools for aquatic product inspection due to their exceptional accuracy and operational efficiency.The study primarily searched multiple academic literature databases, including CNKI,Web of Science,Food Science,IEEE Access Computers and Electronics in Agriculture and Fisheries Research The comprehensive search covered Chinese and English journals,conference proceedings, and dissertations published from 2015 to 2025.Through a systematic review and synthesis of existing domestic and international literature, a complete technical architecture for machine vision-based fish weight assessment was constructed, comprising three core modules:image acquisition devices, feature extraction algorithms, and weight prediction models.High quality image acquisition and high-resolution image data are identified as fundamental prerequisites and critical guarantees for achieving precise weight measurements, leading researchers to compare the advantages, disadvantages, and application scenarios of various acquisition devices.Subsequently, the optimal application ranges of diverse feature extraction algorithms were analyzed, followed by a comparative evaluation of machine vision and deep learning technologies under current environmental conditions.Finally, the study presents concrete prospects and assessments regarding future development trends and research directions for fish weight prediction methodologies.
  • ZHAN Xumin, CHEN Qumei, CHEN Fusheng, DUAN Xiaojie, XIA Yimiao
    Food and Fermentation Industries. 2026, 52(11): 428-438. https://doi.org/10.13995/j.cnki.11-1802/ts.043343
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    During thermal, alkaline, and other processing treatments, proteins often undergo the formation of covalent cross-links, including disulfide bond cross-linking, Maillard reaction-mediated cross-linking, dehydroprotein-derived cross-linking, isopeptide bond cross-linking, and dityrosine cross-linking.The formation of these covalent cross-links can alter the physicochemical and functional properties as well as the nutritional value of proteins, while also leading to the generation of harmful compounds such as 5-hydroxymethylfurfural, lysinoalanine, and dityrosine.Elucidating the mechanisms and applications of protein covalent cross-linking is crucial for optimizing food processing technologies and mitigating food safety risks.Consequently, a comprehensive review and analysis focus on the mechanisms, applications, and health risks of five major protein covalent cross-linking types.Interrelationships between distinct cross-linking types are rigorously dissected, aiming to establish theoretical foundations for protein structural configurations and functional properties.
  • YANG Liu, YU Yanhua, ZHOU Yujie, XIE Sida, ZHAO Ping, YANG Xiaoqin, ZOU Lihua
    Food and Fermentation Industries. 2026, 52(11): 439-450. https://doi.org/10.13995/j.cnki.11-1802/ts.044042
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    Polyphenols, as important representatives of plant-derived functional ingredients, are widely present in various edible plants.Their unique phenolic hydroxyl structure imparts remarkable antioxidant and antimicrobial activities, making them of great value in the development of functional foods.However, traditional organic solvent extraction methods suffer from low extraction efficiency, residual organic solvents, and environmental hazards, severely limiting their application.In recent years, deep eutectic solvents (DESs), as a new green solvent system, have provided an innovative solution for the efficient and green extraction of food polyphenols due to their low toxicity, biodegradability, high solubility, and designability.This article systematically reviews the structural characteristics and functional activities of food-derived polyphenols, the physicochemical properties, preparation methods, and advantages of food-grade DESs, the research progress in DESs extraction technology, key factors, and the mechanism of action of DESs in polyphenol extraction.Despite significant progress in polyphenol extraction technology based on DESs, challenges remain, including low mass transfer efficiency due to high viscosity of the extraction system, lack of theoretical guidance for system design, difficulty separating polyphenols from solvents, and poor recyclability and reuse.Future efforts should focus on integrating molecular dynamics simulation and machine learning to design low-viscosity, high-solubility, green DESs systems.Furthermore, efficient polyphenol-DESs separation and solvent recycling processes based on membrane separation and ice-salt bath condensation should be developed.These strategies will provide theoretical support for the development of safe and efficient polyphenol extraction processes for functional foods, promoting the green and sustainable development of the food industry.
  • QIAN Dongsheng, LIU Boye, CHEN Fusheng, GUO Xingfeng, ZHU Tingwei
    Food and Fermentation Industries. 2026, 52(11): 451-459. https://doi.org/10.13995/j.cnki.11-1802/ts.044151
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    The wet separation technology, represented by the alkali dissolution and acid precipitation method, is the mainstream method for extracting plant proteins, resulting in products with high purity.However, it faces issues such as high energy consumption and environmental pollution.In contrast, the green and environmentally friendly dry separation technology does not require the use of water or chemical reagents, eliminating wastewater discharge.It can better preserve the natural structure and functional properties of proteins, presenting great development potential.To promote the development of green extraction technology for plant proteins, this paper reviews the processing flow and key influencing factors of dry plant protein separation, deeply explores the functional properties of protein products obtained by this technology and their applications in the food industry, and provides an outlook on the future development of dry separation technology.
  • LI Liang, LIU Xiaoru, LI Jingfang
    Food and Fermentation Industries. 2026, 52(11): 460-471. https://doi.org/10.13995/j.cnki.11-1802/ts.045465
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    Lotus products primarily including lotus root and seeds, represent a nutritional and medicinal edible resource.However, they are highly susceptible to enzymatic browning during processing and storage, which severely compromises their appearance, nutritional quality, and market value.Enzymatic browning is a complex process driven by the oxidation and polymerization of phenolic compounds, catalyzed by enzymes such as polyphenol oxidase and peroxidase, leading to the formation of quinones and melanin.Its occurrence is associated with enzyme activity, phenolic substrate content, oxygen availability, and environmental factors like pH and temperature.In recent years, there have been many advances in research on browning control of lotus products.Current strategies can be categorized into physical methods (e.g., low-temperature storage, high-pressure processing, pulsed electric field treatment), chemical methods (e.g., application of organic acids, slightly acidic electrolytic water), and biological methods (e.g., using natural extracts, probiotic metabolites).Notably, combined control techniques demonstrate promising potential due to their synergistic effects.This review summarized the core mechanisms of enzymatic browning in lotus products and examined the current status, mechanisms, and application efficacy of various control methods.The review proposed future directions focused on developing natural, efficient, and green-oriented browning inhibition strategies, aiming to provide a theoretical foundation for enhancing product quality in the deep-processing lotus industry.