25 December 2017, Volume 43 Issue 12
    

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  • ZHAO Wei-xin et al
    Food and Fermentation Industries. 2017, 43(12): 1-6. https://doi.org/10.13995/j.cnki.11-1802/ts.014923
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    Self-assembling amphipathic peptides (SAPs) are a kind of functional peptides, which have alternating hydrophilic and hydrophobic residues and can affect the thermal stability and catalytic properties on the fused enzymes. In this study, several SAPs fusion protein which comprised S1 peptide (AEAEAKAKAEAEAKAK) derivatives and Bacillus sp.WSHB04-02 alkaline polygalacturonatelyase (PGL) or PGL-S1 with different linker peptides were constructed, and the effect of the linker peptides and the amino acids composition of SAPs on expression quantity the fusion enzymes were examined. The results showed that the hydrophobicity of hydrophobic amino acids make a critical difference on the expression quantity of PGL. The SAPs which contained the weaker hydrophobic alanine and glycine residues could make the fused protein expressed normally. The PGL-S1v1 possessed the relative high crude enzyme activity. Compared with the PGL and PGL-S1, the extracellular enzyme activity was increased by9-fold and 1.5-fold, respectively. As for the linker peptides, the flexible linkers enhanced the extracellular expression of the fusion protein more efficiently compared with the rigid counterparts. Of which the crude enzyme activity of PGL-F-S1 with flexible linker peptide were increased 14-fold than that of PGL. These results suggested that the amino acids composition of SAPs and the flexibility of the linker peptides could effectively affect the expression quantity the fusion protein.
  • CHEN Peng-cheng et al
    Food and Fermentation Industries. 2017, 43(12): 7-12. https://doi.org/10.13995/j.cnki.11-1802/ts.015305
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    In this paper, sugarcane bagasse residue, polypropylene microfiber membrane and cotton cloth were adopted as supports to immobilizeActinobacillussuccinogenesfor the fermentative production of succinic acid. The usage of supports was optimized to improve the yield of succinic acid and repeated batch fermentation was conducted to study the reusable stability of supports. Polypropylene microfiber membrane performed best in the production of succinic acid while cotton cloth performed best in the reusable stability test. As a result, cotton cloth was used in the construction of a fibrous-bed bioreactor. In the study of the effect of pH regulator on bioreactor efficiency, there were 32% and 20% increase in succinic acid concentration when using MgCO3as the pH regulator compared with that using NH3×H2O-Na2CO3and KOH-K2CO3. Repeated fed-batch fermentation was operated in the bioreactor with the average batch time of 35 h and the total operation time of 175 h. Results showed that the average concentration, productivity and yield of succinic acid were87.8 g/L, 2.51 g/L×h and 0.86 g/g, respectively. Results showed that the fibrous-bed bioreactor reported in this paper helped to achieve high concentration, high productivity and high yield of succinic acid.
  • LIU Li-li et al
    Food and Fermentation Industries. 2017, 43(12): 13-19. https://doi.org/10.13995/j.cnki.11-1802/ts.015222
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    Collagenase is a kind of enzymes thatspecificallyhydrolyze natural collagen or gelatin. In order to prepare a new type of collagen protease and explore its ability to degrade collagen,a novelbone specific collagenase (BSC) was obtained fromBacillus cereuscrude enzyme liquid throughthe purification steps of ammonium sulfate precipitation, DEAE-Sepharose Fast Flow ion exchange chromatography and Sephadex G-100 gel chromatography. Furthermore, itsmolecular weight of the purified enzyme, substrate specificity and degradation ability were analyzed. What’s more, we derived the kinetic model of hydrolyzing bovine bone collagen. The results showed that the specific activity of BSC was 5.57×103U/mg, the purification times was up to 42.85 times, and its molecular weight was about 52.0 kDa. The specificity analysis showed that the substrate was a bone collagen enzyme, and its ability to hydrolyze type I collagen was significant.The comparative test indicated that the hydrolysis capacity of BSC was higher than that of other proteases.The dynamic model of the enzymatic degradation of collagen was:the degradation rate,degree of hydrolysis,Enzyme inactivation constant K4=64.1157 h-1. Theresults provided a novel collagenolytic protease for the development of animal bone collagen industrial.
  • HE Xiao-yong et al
    Food and Fermentation Industries. 2017, 43(12): 20-24. https://doi.org/10.13995/j.cnki.11-1802/ts.015196
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    In order to improve the stability of laccase, the effects of three additives(glycerol, inositol and sorbitol)on the acid-base stability of laccase were studied, and the protection mechanism of the additives on laccase was analyzed by UV spectroscopy, fluorescence spectroscopy and circular dichroism. The results indicated that the protective effect of the additives on laccase was related to the type and concentration of the additives. When the concentration of inositol was 0.2 mol/L in buffer at pH = 3, the relative activity of laccase reached the maximum(125%). With increasing the concentration of glycerol or sorbitol, the relative activity of laccase increased gradually. When the concentration was 0.6 mol / L, glycerol increased the relative activity of laccase by 29%, and sorbitol increased the relative activity of laccase by 15%. The optimum pH range for laccase should be at pH = 2 to 5, and the use of additives could improve the acidity of laccase. Spectral analysis showed that these additives could form hydrogen bonds with laccase molecules in the solution, resulting in the improvement on the acid-base stability of laccase.
  • YANG Fang et al
    Food and Fermentation Industries. 2017, 43(12): 25-29. https://doi.org/10.13995/j.cnki.11-1802/ts.015518
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    The study was aimed to investigate the change of antioxidant activities in silver carp surimi during fermentation and clarify the reason for its production by correlation analysis to degree of hydrolysis (DH), free amino acids and molecular weight distribution. The results showed that ABTS scavenging activities and Fe2+chelating activities increased during fermentation, while DPPH scavenging activities grew up firstly and then went down. Meanwhile, DH and free amino acid content increased, with upward trends in content of low-molecular-weight peptides and hydrophobic amino acids. Correlation analysis showed that Met, Cys and peptides in the range ofMr1000-5000 may be the reason for the production of antioxidant properties.
  • YUAN Kai et al
    Food and Fermentation Industries. 2017, 43(12): 30. https://doi.org/10.13995/j.cnki.11-1802/ts.014490
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    Based on the technology of industrial surimi washing , this paper discusses the law of protein oxidation in the process of surimi processing, and provides scientific basis for surimi processing. At different temperatures (4, 12, 20℃) and different washing times (1, 2, 3), fresh silver carp surimi surimi had been prepared. Then the content of carbonyl group and total thiol content, Ca2+-ATPase enzyme activity, the degree of protein degradation and aggregation, chang of ssecondary protein structure would be detected. The results show that the content of carbonyl increased significantly with the increase in temperature, however increasing washing times decreased it. and the disordered structure increase with the increase in temperature and times, while the content of total sulfhydryl andβ-fold structure decreased gradually; Myosin degradation reaction exacerbated caused by washing treatment with increasing temperature; protein solubility and Ca2+-ATPase activity decreased; Among these indexes, the SDS-PAGE pattern and the changes of the secondary protein structure could be good indexes to evaluate oxidation denaturation. Washing 1-2 time at 4℃could effectively control protein oxidation degree while ensuring yield of protein.
  • ZHAO Wei et al
    Food and Fermentation Industries. 2017, 43(12): 37-43. https://doi.org/10.13995/j.cnki.11-1802/ts.015409
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    Monascusspp. is a traditional Chinese medicinal and edible microbial resource. Its main metabolites includingMonascuspigment (MPs), monacolin K (MK), etc. The previous results indicated that glycerol could improve MK and MPs production byMonascus. AMonascusstrain,Monascus pilosusMS-1, which could produce high yields of MK and MPs but non citrinin was used. And the effects of crude glycerol and analytical grade glycerol on the growth and production of MK and MPs by MS-1 were carried out. The results showed that analytical grade glycerol could improve MK and MPs production in solid-state fermentation by MS-1. The most suitable analytical grade glycerol concertations for MK and MPs produciton in solid-state fermentation was 6% and 3%, respectively, and the yields of MK and MPs was 1.57 and 1.53 folds to that of control, respectively. The highest yields of MK and MPs were obtained when 6% crude glycerol from fatty alcohol with 90% purity was used and the homologues were also more than that of control and which adding analytical grade glycerol in solid-state fermentation (p<0.05). In liquid fermentation, pH values of fermentation broths and biomass were significantly decreased and increased, respectively, when crude glycerol and analytical grade glycerol with concentration of 40 g/L were used (p<0.05). MK and MPs production were significantly enhanced when crude glycerol and analytical grade glycerol were used (p<0.05). MK and MPs yields were 11.57 and 11.08 folds to that of control when crude glycerol from biodiesel with 90% purity and crude glycerol from fatty alcohol with 90% purity were used, respectively. Moreover, the species of MK and MPs were increased compared to that of control. Crude glycerol possess huge potential use for MK or MPs production.
  • LI Juan et al
    Food and Fermentation Industries. 2017, 43(12): 44. https://doi.org/10.13995/j.cnki.11-1802/ts.013880
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    Millet bran, rice bran, wheat bran, brown rice, sticky rice were wet pulverized by the high-pressure microchannel fludizer.The effects of raw material, pressure and cycle times on particle sizes of the samples were investigated.Effects of the high-pressure microchannel fludizer on the microbial reduction were also studied. The experimental results showed that the particle sizes of millet bran, rice bran, wheat bran, brown rice and sticky rice were reduced significantly after the high-pressure microchannel fludizer treatments.Especially, the particle size was remarkably reducedafter the first cycle. The average particle diameter D50of brown rice and sticky rice was reduced to less than 10 μm after the high-pressure microchannel fludizer treatments. The results showed that the total number of microbialdecreased by 20 times, and the high-pressure microchannel fludizer played a role in bacteria-reducing. Therefore, the shelf-life of liquid products can be extended.
  • LI Tian-qi et al
    Food and Fermentation Industries. 2017, 43(12): 49-54. https://doi.org/10.13995/j.cnki.11-1802/ts.014389
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    In this paper, natural starch was used as raw material to synthesize starch based heavy metal trapping materials by stepwise molecular modification, and the trapping ability of copper and nickel ions was evaluated. The starch molecules first introduce cationic quaternary ammonium groups through etherification. On this basis, continue to introduce the phosphorylation of anionic groups. The addition of urea in the synthesis process could promote the cross-linked structure of various types of groups and introduced non ionic groups. Finally, modified starchproducts with multiple charge distribution were obtained. The results showed that the dosage of CHPTAC was 4%(mL/g) of starch quality, reaction temperature was 50℃ for 4h, the degree of cation substitution was 1.61%.The mass concentration of sodium tripolyphosphate was 4%, pH value as 6-7, reaction temperature was 120℃ for 2h, the degree of substitution of negative charged could reach0.026.Low concentration of urea can improve the reaction efficiency of phosphorylated starch. When the mass percentage concentration is higher than 3%, it can enhance the cross-linking of starch with the molecular carbamate. The products with different degree of substitution have a certain trapping effect on the copper and nickel ions. When the cationic and anionic phosphate esterification degree of substitution were 1.421% and 0.018, the removal rate of 30 mg/L copper and nickel ions solution could reach 98%.
  • GONG Wei-hua et al
    Food and Fermentation Industries. 2017, 43(12): 55-60. https://doi.org/10.13995/j.cnki.11-1802/ts.014627
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    Lignin was extracted from bamboo (Dendrocalamus latiforus)shoot shell by acetosolv process. The biological activities of lignin on glucose dialysis retardation index (GDRI), fermentation property and enzyme activity were investigated and compared with the same source of cellulose residues and coarse dietary fiber. The results showed that the GDRI ability of lignin was relatively low, but the fermentation properties were enhanced. The pH value in the fermentation liquid was decreased, the content of short-chain fatty acid(acetic acid,propionic acid and butynic acid) in the fermentation liquid was increased. In the enzymatic activity, the lignin activated the α-amylase activity and can be used as an activator of α-amylase. But there were no obvious influence on the lipase activity. Therefore, lignin has a positive effect onfermentation properties and α-amylase activity of dietary fiber of bamboo shoot shell.
  • LI Juan et al
    Food and Fermentation Industries. 2017, 43(12): 61-65. https://doi.org/10.13995/j.cnki.11-1802/ts.013722
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    The black millet has been favored because of its unique color, rich in nutrients and beneficial to human health. The differences between components of black millet and yellow millet were analyzed in this work. Besides, the rheological characteristics of black and yellow millet were measured by Mixolab in order to evaluate the functional properties of protein and starch in millet. The product of millet crust were also prepared by three different processing techniques. The results showed that the black millet has a higher nutritional value than the ordinary yellow millet, especially in protein, oil and mineral salts content. The results of Mixolab showed that the black millet had a relative good protein stability, starch thermostability and processing characteristics. Our work will supply scientific evidences and technical supports in application of black millet and its products in future.

  • ZHANG Li et al
    Food and Fermentation Industries. 2017, 43(12): 66-70. https://doi.org/10.13995/j.cnki.11-1802/ts.013399
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    Astilbin is a unstabledihydroflavonol anditsisomerizationand decomposition is found in neutral and weak alkaline medium. In the present study, the effects of different food components on the stability of astilbin wasstudied by HPLC. The results showed that common food additives, VC、saccharose and β-cyclodextrin (β-CD) could significantly improve its stability, while sodium benzoate has no effect. β-CD could suppress the isomerization and decomposition of astilbin through complexation; while VC and saccharose accelerate the isomerization but suppress the decomposition of astilbin. Metal ions, such as Al3+、Mg2+、Ca2+、Na+、K+could slightly improve the stability of astilbin, and Na+showed best effect. Fe3+significantly improved the decomposition of astilbin and decreasedits stability; Al3+completely inhibited the isomerization of astilbin but has no effect on its decomposition. The major components of food, protein (bovine serum albumin) and starch, have slight effects on the stability of astilbin.
  • AI Xin et al
    Food and Fermentation Industries. 2017, 43(12): 71-74. https://doi.org/10.13995/j.cnki.11-1802/ts.015112
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    In this study, the physicochemical characteristics (color, odor, pH value, acidity and milk composition)of goat milk during lactations, theircoagulation characteristics by chymosin(hardness, cohesiveness and elasticity) andwater holding capacities were investigated to provide the suitable materials for the development of goat milk products. The results showed that the physicochemical characteristics of goat milk during lactations and theircoagulation characteristics varied. With the prolongation of lactation, the color of goat milk gradually changed from milky yellow to milky white, and the odor of goat faded, but pH values graduallyrised.The acidity, density, its main chemical components, the coagulation characteristics andwater holding capacities all declined. By comprehensive analysis, goat milk at 120 - 150 d lactation were the better materials for the production of cream, while the milk at 4 -150 d lactation would be suitable for the production of set yogurt and cheese. Goat milk at 7 - 240 d lactation could be used for the production of liquid milk or the milk powder, but the standardization process should be needed.
  • LUO Cheng et al
    Food and Fermentation Industries. 2017, 43(12): 75-80. https://doi.org/10.13995/j.cnki.11-1802/ts.015053
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    L.plantarumFCJX 102 andL. acidophiluFCJX 104 producing acetaldehyde dehydrogenase were selected from five kinds of lactic acid bacteria including Lactobacillus brevis, Lactobacillus paracasei, Lactobacillus plantarum, Lactobacillus acidophilus and Lactobacillus rhamnosus. On the basis of single factor experiment, the fermentation conditions of hawthorn puerarin juice producing aldehyde dehydrogenase were studied by single factor and orthogonal optimization, hawthorn pueraria ratio, strain ratio and fermentation time as impact factors. Experimental results showed that when the rate of hawthorn and puerarinin the medium was 1:2 and the amount ratio ofL. plantarumFCJX 102 andL. acidophilu FCJX104 was 2:1, and cultured at 37 for 16,the highest enzyme activity of acetaldehyde dehydrogenase was obtained after optimization was 16.083U / g.
  • JIANG Dong-lei et al
    Food and Fermentation Industries. 2017, 43(12): 81-87. https://doi.org/10.13995/j.cnki.11-1802/ts.015111
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    Due to the safety hazard of chemical synthetic antioxidants, the application of natural antioxidant in meat processing has become a hot research topic. In this study, the ethanol extracts of cinnamon was prepared by distillation and applied to the chinese-style sausageto study the influence on the physical and chemical quality of fermented sausage, as well as the antioxidant effect of lipid and protein.The results showed that the pH value increases after the first decrease, the Aw values of test groups with 0.3% and 0.6% cinnamon extracts maintained at about 0.72 in 28 days. Next, The L * and a * values of cinnamon extracts group was significantly higher than the blank group. Finally, the antioxidation effect for lipid and protein of each treatment group were also obviously higher than that of blank group and were correlation with the addition of extracts, in which the 0.6% of cinnamon extracts group has the same antioxidant effect of 0.02% BHT. Therefore, the cinnamon extracts can effectively inhibit associates value and carbonyl increased in chinese-style sausage so as to improve the quality of sausage.  Therefore, cinnamon extracts can be selected as an alternative for the synthetic antioxidants to a certain extent.
  • ZHANG Dong et al
    Food and Fermentation Industries. 2017, 43(12): 88-92. https://doi.org/10.13995/j.cnki.11-1802/ts.014293
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    To shorten the pickling time, reduce production costs, to guide the rapid bacon pickled, this study evaluated the atmospheric pressure rolling pickling, vacuum roll rubbing, pulsating vacuum roll rubbing on the quality of pork and curing rate samples including pH, marinade/meat ratio, cooking loss, shear force and salt content, with atmospheric pressure pickling as the control. The results showed: In the four kinds of pickling methods, the pH value of pork showed an increasing trend, but the difference between adjacent test points was not significant (P>0.05); pulsating vacuum roll rubbing, vacuum roll rubbing and atmospheric pressure rolling pickling the marinade/meat ratio is higher than atmospheric pressure pickling, the marinade/meat ratio of pulsating vacuum roll rubbing is the largest; The cooking loss and shear force of pulsating vacuum roll rubbing was minimal compared to the other three kinds of pickling methods; To achieved the same salt content, pulsating vacuum roll kneading for shortest time. Finally showed: Pulsating vacuum roll kneading marinated pork effect is better.
  • SUN Yan et al
    Food and Fermentation Industries. 2017, 43(12): 93-98. https://doi.org/10.13995/j.cnki.11-1802/ts.014673
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    Wheat flour was used as material to be extruded and made for koji of white soy sauce. The glucoamylase activity of koji is regarded as the index. The effect of different extrusion parameters on the glacoamylase activity of koji were studied. By single factor experiment, the effect of the moisture content of flour, screw speed, feeding spend and barrel temperature on the glucoamylase activity were investigated. Then the extrusion parameters were optimized by Response Surface Methodology (RSM). The results showed that the optimal glucoamylase activity was 1293.49 U/gunder the following extrusion conditions, including moisture content of 40%, screw speed of 100 r/min, feeding speed of 10kg/h, and barrel temperature of 80 °C. The koji of higher glucoamylase activity was utilized to ferment and the brewing experiment of white soy sauce was conducted by low-salt solid-state fermentation. After the end of fermentation, the contents of amino acid nitrogen and reducing sugar were 0.65 g/100mL and 243.25 mg/mL respectively in the white soy sauce. The white soy sauce tasted sweet and showed good flavor.
  • CHEN Xiao-long et al
    Food and Fermentation Industries. 2017, 43(12): 99-107. https://doi.org/10.13995/j.cnki.11-1802/ts.014711
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    This study synthesized the molecularly imprinted polymers by using the molecular structure analogues of α-(OH)-sanshool as the template molecule, 2-Vpy as functional monomer, EDMA as crosslinking agent at the mole ratio of 1:4:20, and then the molecularly imprinted solid-phase extraction (MISPE) was prepared. The optimal sample solvent, drip washing solvent and elution solvent were selected out and the SMIP was used to separate and purify the numb taste components in Zanthoxylum oleoresin, the results show that the SMIP had the especially specific adsorption to the numb taste components which the relative purity of numb taste components can add to 93.66%.
  • GUO Yu-cheng et al
    Food and Fermentation Industries. 2017, 43(12): 108-113. https://doi.org/10.13995/j.cnki.11-1802/ts.015155
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    Different types of fermented wines were made of “Niuxin” and “Huojing” persimmons separately by fermentation in low temperature. The quality characteristics of the fermented persimmon wines were investigated, and the phenolic compounds and the antioxidant activity were also examined. The results showed that, the average juice yield of persimmon fruit was about 55%, all the physicochemical indexes except higher total acid contents and the sensory quality of all the made wines were in line with the corresponding national standards. The fermented persimmon wines had higher phenolic compounds and stronger antioxidant activities, as gallic acid was the richest (above 80%) of the individual phenolic compounds determined, and there was a significant positive correlations between total phenols and ABTS+· radical scavenging activity (r=1,p<0.01), while a negative correlation between total flavanol content and DPPH radical scavenging activity (r= -1,p<0.01). Comparing with commercial Chardonnay dry white wine, persimmon wine seems to be much richer in total phenolics with higher antioxidant activity. It indicates that further work on developing fermented persimmon wine is promising in future.
  • DUAN Zhen-zhen et al
    Food and Fermentation Industries. 2017, 43(12): 114-123. https://doi.org/10.13995/j.cnki.11-1802/ts.015026
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    In this paper, with red flesh loquat as raw material, the effects of organic acids and volatile flavor substances change on loquat vinegar quality were studied by using high performance liquid chromatography (HPLC) and solid-phase microextraction (SPME) combined with gas chromatography-mass spectrometry (GC-MS). The results showed that the organic acid content increased significantly in the processing of loquat vinegar, acetic acid was the main acid of loquat vinegar, and malic acid, lactic acid, citric acid, succinic acid and tartaric acid were the accessory acid characteristic components of loquat vinegar. The content of citric acid and succinic acid was increased by artificial aging,which made the sour taste of loquat vinegar more prominent. Volatile aroma substances analysis showed that carbonyl compounds were the main volatile aroma compounds in loquat fresh juice, alcohols and esters were the main volatile aroma compounds in loquat wine and vinegar. The content of isoamyl alcohol was significantly reduced by artificial aging,which can improve the flavor quality of loquat vinegar. There are 9 kinds of aroma in fresh loquat vinegar, 6 kinds of aroma after accelerating aging. The main aroma components are phenethyl alcohol and ethyl acetate, a variety of aroma components make up the fragrance characteristics of loquat vinegar which includes esters, vinegar, andflowers and fruitaromas.Ultrasonic aging is better than microwave aging.
  • CHEN Mei-hua et al
    Food and Fermentation Industries. 2017, 43(12): 115-124. https://doi.org/10.13995/j.cnki.11-1802/ts.014523
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    Taking water content, oil content, color, sensory score and oil uptake as indexes, the effect of frying temperature(165,175,185℃)and time(60,90,120,150,180 s)on quality of fried, battered, and breaded oyster were evaluated. The results showed that with the increase of frying temperature and time, the water content decreased, oil content increased,L* andb* decreased,a* increased, sensory score increased first and then decreased, oil uptake increased. In addition, battered and breaded oysters fried at 175 ℃for 90 s or 120 s were with golden-brown surface color, crunchy crust, tender core, and outstanding characteristic flavor of oyster, so theirsensory quality were the best.
  • ZHANG Peng et al
    Food and Fermentation Industries. 2017, 43(12): 130-136. https://doi.org/10.13995/j.cnki.11-1802/ts.014266
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    In order to study the box-type spontaneous atmosphere ofActinidia argutain the most suitable period of cold storage and to improve the storage and transportation ofActinidia argutaand ensure the fresh quality ofActinidia arguta, three different atmospheric elements (12, 7, 6) Effects of spontaneous formation of microenvironment gas content, fresh quality and electronic nose on the fruit of 'Yangtze River No.1'. The results showed that the decay rate, soluble solids content, titratable acid content and Vc content of the kiwi fruit were significantly higher than those of the control group (CO2: 4.3% - 5.0%, O2: 15.9% - 16.4%) (CO2: 3.7% - 4.7%, O2: 16.2% - 17.1%) was lower than that of the other two components in the effect of slowing down the hardness and decreasing the respiration intensity and ethylene production rate. And 7 (CO2: 2.6% - 3.3%, O2: 17.6% - 18.3%) were better than CK group, and the decay rate, hardness, content of sugar and acid, Vc content, respiration intensity and ethylene production rate were the best, and there was significant difference between each index and CK group (P<0.05). In addition, it is possible to obtain the results of PCA and linear discriminant analysis (LDA) analysis of the processedActinidia argutaby electrons. After a period of storage, the flavor components of element 7 are still the closest to the initial flavor, and LDA It is clear that the flavor of the CK group is different from that of the three different air conditioning elements (12, 7, 6). In summary, the No. 12 air conditioning element preservation effect is the worst, 6,7 gas regulator can inhibit the occurrence of decay rate, delay the reduction of titratable acid and Vc content, delay the fruit ripening to ensure its soluble solids content , The inhibition of fruit respiration peak and ethylene production rate, and the No. 7 gas conditioning element is most suitable for storage ofActinidia argutaduring storage.
  • LIU Cai-hua et al
    Food and Fermentation Industries. 2017, 43(12): 137-143. https://doi.org/10.13995/j.cnki.11-1802/ts.014470
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    The changes of textural properties (Hardness, adhesiveness, springiness, cohesiveness, gumminess, chewiness and resilience) and shear force ofEsox luciusunder different refrigeration temperature (4?℃and 0?℃) were measured by Texture Profile Analysis (TPA) model and the Warner-Brazler of texture analyzer, meanwhile, changes of pH value, electroconductivity and water-holding capacity were studied. The results obtained from this study reveal that pH value of theEsox luciusmuscle showed a downward trend in earlier stages of storage and then showed a upward trend with the storage time increased, while electroconductivity and adhesiveness generally showed a upward trend during the whole storage period, and the two indicators ofEsox luciusmuscle stored at 0?℃ were less than that stored at 4?℃, what’s more, water-holding capacity, hardness, springiness, cohesiveness, gumminess, chewiness, resilience and shear force of the samples showed a declining trend at vary degrees, and these indicators under 0?℃ were significantly higher than those under 4?℃.
  • GU Ren-yong et al
    Food and Fermentation Industries. 2017, 43(12): 144-149. https://doi.org/10.13995/j.cnki.11-1802/ts.014587
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    Effects and conditions of stable chlorine dioxide combined with heat treatment were explored to prolong the storage time of Chestnut. With preservation rate as index, a response surface methodology was used to investigate the effects ofChlorine Dioxide mass concentration, heat treatment temperature and time on the preservation rate to optimize the storage conditions.The results showed that synergy effects existed between Chlorine Dioxide mass concentration and treatment temperature and between Chlorine Dioxide mass concentration and treatment time. The best storage conditions wereChlorine Dioxide mass concentration 167 mg/L ,treatment temperature 57 ℃ , treatment time 22 min. Under the conditions,after storage at temperatures of 5-15 ℃ for 60 days, the preservation rate of Chestnut was up to 93.61%。
  • WANG Wen-xia et al
    Food and Fermentation Industries. 2017, 43(12): 150-156. https://doi.org/10.13995/j.cnki.11-1802/ts.014465
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    Four potato pectin polysaccharides were extracted from potato residue which had been adopted the enzymatic removal of starch and protein by hydrochloric acid solution , citric acid solution, alkaline phosphate solution and compound salt solution respectively; furthermore, ultra-filtrated, precipitate by ethanol and lyophilized. Effect of different extraction methods on yield, the composition characters of potato pectic polysaccharide were researched. The results show that the bigger extraction yield, produced by alkaline phosphate and compound salt extraction ,were 29.89% and 21.01% , respectively. The bigger galacturonic acid content of pectic polysaccharide that was used alkaline phosphate solution and compound salt solution were 29.71% and 31.57% , respectively. The ash content of pectic polysaccharide that was used alkaline phosphate solution (22.38%) is significantly higher than other three kinds of one (2.09%-2.48%). Pectic polysaccharide extracted by four kinds of processing methods was of low-methoxyl pectin. There was no significant difference between protein content of four kinds of pectic polysaccharide (less than 3.0%).Galactose was the major neutral monosaccharide units along with a small portion of arabinose, rhamnose and glucose. The molecular weights (Mp) of four pectic polysaccharide that was hetetopolysaccharide ranged from 1.65×104u to 1.17×106u.The infrared spectra showed that all pectins extracted from potato had typical functional groups of pectin. The research has theoretical and practical significance of development and production of potato pectin.
  • ZHANG Bi-ying et al
    Food and Fermentation Industries. 2017, 43(12): 157-163. https://doi.org/10.13995/j.cnki.11-1802/ts.014531
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    In order to investigate the effect of different extracting juice technologies on quality and antioxidant activity of sweet potato juice, four pretreatment methods were selected.The light transmittance, color, sensory quality and main nutritional ingredient(protein,Vc,β-carotene,toal phenolics,flavonoids) were compared and analyzed. The antioxidant activity of sweet potato juice was evaluted by means of five vitro antioxidant systems(DPPH,ABTS+,·OH,·O2-radical scavenging and total reducing force mathod).The results showed that The retention rate of sweet potato juice and the main components after atmospheric cooking were slightly inferior to microwave cooking, but its color and stability were better. The nutrient loss of sweet potato juice was the highest after the high pressure cooking, and the loss rate of vitamin C and β-carotene was Up to 92.54% and 72.25%, and the browning is serious.Moreover, after screw extrusion,the nutrient retention rate of sweet potato juice was the highest, and the storage rate of soluble protein was 94.55%, the antioxidant activity was high and the comprehensive quality was good.

  • LI Su-lin et al
    Food and Fermentation Industries. 2017, 43(12): 164-168. https://doi.org/10.13995/j.cnki.11-1802/ts.014158
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    hecultivationandaplicationofthemedicinalplantsofPanaxginsenginChina,includingPanax notoginsenghasalonghistoryanditisofgreatsignificancetothedevelopmentofthesepreciousresourcesbyfermen tation.Inthispaper,westudiedthefermentationprocesesoftheserawmaterialswithmicrobialstrainsbelongtoLac tobacilusbulgaricus,Baciluscoagulans,LactobacilusrhamnosusandEurotium cristatum etc.whichcommonlyused infod industry. HPLC wasused toanalyzethechangesofginsenosidecompositon afterdiferentfermentaion. Throughthefermentationwithdiferentmicrobialstrains,Rb1,Reandothercommonginsenosidewhichcontainedin Panaxnotoginseng,ginsengandAmericanginsengwerebiotransformedrespectivelyintoRh1,CK andRd,while ginsenosideRh2wasnotdetectedintheproduct.Theresultssugestedthattheglycosidasesfrom thesestrainswere veryspecificandprovidedabasisforthebiotransformationandutilizationofspecialtargetsaponins.
  • DANG Yan-ting et al
    Food and Fermentation Industries. 2017, 43(12): 169-175. https://doi.org/10.13995/j.cnki.11-1802/ts.014722
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    In recent years, Chinese Maca industry has been through a roller coaster development, along with the price skyrocketed and plummeted. When Maca held at high price, the phenomenon adding the inferior powder (corn flour, radish powder and turnip powder etc.) into commercial Maca powder was overwhelmingly universal. In the present study, the microstructures of corn flour, radish powder, turnip powder and Maca powder were investigated by desktop scanning electron microscopy (SEM), and elemental compositions of turnip and Maca powder were analyzed. The result illustrated that radish powder and corn flour were evidently different from the micro-morphology of Maca powder. Turnip powder was the closest to Maca powder, but there were still apparent

    differences. By X-ray energy spectrum analysis, the result showed: if N and O element mass ratio were close to 43.75% ± 0.79% and 29.92% ± 0.74% respectively, the sample was considered pure Maca powder preliminarily; otherwise the measured was suspected to fake Maca powder .Meanwhile, electronic nose was used to detectedMaca powder adding different proportions turnip powder. It was suggested that the fingerprints of Maca powder with distinct degree adulteration appeared a certain regularity. Electronic nose not only could effectively identify the authenticity of Maca powder, but might also roughly determine the proportional range of adulteration. Additionally, quality control of raw materials and products could be attained by statistical quality control (SQC) analysis model.
  • LIU Pan-pan et al
    Food and Fermentation Industries. 2017, 43(12): 176-183. https://doi.org/10.13995/j.cnki.11-1802/ts.014144
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    Fresh tea leaves plunked from Chibiquntizhong in Chibi tea area were taken as raw materials to make Qingzhuan tea. Volatile components of Qingzhuan tea during the primary and pile fermentation process were analyzed using headspace solid-phase micro-extraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS). The result showed that 62 volatile compounds were identified from Qingzhuan tea samples, while 45, 41, 41, 40, 43, 42 and 48 compounds were identified from fresh leaves to the end of pile fermentation 7 samples in total. In theprevious stage of pile fermentation, aldehydes, alcohols, ethers and ketones had a relatively higher content. However, the relative amount of olefin aldehydes, ketones, methoxybenzene and furans significantly increased, alcohols and ethers obviously decreased which caused by microbial action during pile fermentation. Twelve high OAV (>1) ofβ-ionone, linalool, (Z)-4-heptenal, (E,Z)-2,6-nonadienal, decanal, (E)-2-nonenal, (E,E)-2,4-heptadienal, 1-octen-3-ol, nonanal,α-ionone, 1-penten-3-one and 2-octen-1-al indicated their importance to the aroma character of Qingzhuan tea in the different process stages. Pile fermentation was the key step in the formation of volatile flavor compounds in Qingzhuan tea. The characteristic aroma components of Qingzhuan raw tea were include (E,E)-2,4-heptadienal, 2-octen-1-al, (E,Z)-2,6-nonadienal, nonanal, benzeneacetaldehyde,β-cyclocitral,β-ionone,α-ionone, 1,2,3-trimethoxybenzene andα-terpineol, et

  • JIANG Bao et al
    Food and Fermentation Industries. 2017, 43(12): 184-190. https://doi.org/10.13995/j.cnki.11-1802/ts.015255
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    Volatile compounds of young Cabernet Sauvignon wines from the flat and slope lands grown in Loess Plateau region (China) were firstly investigated in this research. Among the volatile compounds analyzed by HS-SPME with GC-MS, a total of 43 and 45 volatile compounds were identified and quantified in the flat and slope lands wines, respectively. In the volatiles detected, higher alcohols formed the most abundant group in the aromatic components of the 2 wines, followed by esters and fatty acids. According to their odor active values (OAVs) and relative odor contribution (ROC), the aromatic profiles for the 2 wines were similar, showing only quantitative but not qualitative differences. Ethyl octanoate, ethyl hexanoate and isoamyl acetate were found to jointly contribute to 98.8% and 99.2% of the global aroma of the flat and slope wines, respectively. These odorants are associated with “fruity’’ and ‘‘ripe fruit’’ odor descriptors.
  • TAN Xiao et al
    Food and Fermentation Industries. 2017, 43(12): 191-198. https://doi.org/10.13995/j.cnki.11-1802/ts.015173
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    Flavors change of mulberry wine which was fermented by Saccharomyces cerevisiae JM037 posessing γ-aminobutyric acid(GABA) production capacity were analyzed during fermentation process. The changes of organic acid and volatile compounds were evaluated by high performance liquid chromatography (HPLC) and head-space solid phase micro-extraction associated with gas chromatography-mass spectrometry (HS-SPME/GC-MS), respectively. Meanwhile, the production of GABA in mulberry wine was measured. The results showed that 7 kinds of organic acids were detected, and the content of them were firstly increased and then decreased, totally; 54 volatile compounds were identified in mulberry wine, including 19 esters, 16 acohol, 8 aldehydes, 3 ketones, 3 phenols, 4 others., and the relative content of volatile compounds from great to small was as follows: alcohols, esters, ketones, aldehydes and phenols. Moreover, the kinds and relative content of volatile compounds were changed during fermentation process. Finally the principal component analysis (PCA) demonstrated that aldehydes, ketones and phenols of mulberry provided the precursor substance for other flavor compounds of the fermented mulberry wine. Furthermore, the concentration of GABA in mulberry wine reached to 1 180 mg/L.
  • QI Ning-li et al
    Food and Fermentation Industries. 2017, 43(12): 199-204. https://doi.org/10.13995/j.cnki.11-1802/ts.015034
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    A new method based on ultra performance convergence chromatography (UPC2) was developed for analysis of free amino acids in fruit wine. They were separated on an Acquity UPC2Torus Diol (3.0 ×100mm, 1.7 μm) with a mobile phase consisted of supercritical CO2and methanol: acetonitrile (1: 1 v/v, with 2.5 mmol/L ammonium acetate as mobile phase modifier), the flow rate is 2.0 mL/min and DAD detection was set at 280 nm with a compensation range of 330-430 nm, back pressure is 1800 psi. The results showed good linearity (R2= 0.9990-0.9998) and high resolution higher than 1.22. The limits of detection (LOD) and quantification (LOQ) ranged from 23-57 ng/L and 70-178 ng/L, respectively. The linear range was between 20 and 400 μg/mL. The spiked recoveries were ranged from 85.6% to 101.12%. It was applied for the free amino acids analysis of selected pitaya wine, which could also provide a new method for the analysis of free amino acid distribution in other food matrix from different sources.
  • LIU Sheng-nan et al
    Food and Fermentation Industries. 2017, 43(12): 205-208. https://doi.org/10.13995/j.cnki.11-1802/ts.014455
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    Seven different areas of salted fish were being used for investigating the volatile components by electronic nose technique. An e-nose technology was established through principal, linear discriminant analysis  and loadings analysis, and combined with sensory evaluation to analysis the quality of seven samples. The results showed that Qingdao-Shandong salty fish had the highest score of sensory evaluation. The principal component analysis  was more effective than linear discriminant analysis, and the content of total volatile base-nitrogen (TVB-N) plays an important role in distinguish salted fish.
  • YANG Li et al
    Food and Fermentation Industries. 2017, 43(12): 209-213. https://doi.org/10.13995/j.cnki.11-1802/ts.014402
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    PurposeTo establishment an objective method for detecting the brittleness of Sichuan traditional pickled cowpea.MethodThe brittleness detection was carried out with the puncture mode of the TA.XT Plus texture analyzer, and the test conditions as follows: P/2N-2 mm stainless steel needle as test probe, pre-test speed was 1.0 mm / s, test speed was 3.0 mm/s, post-test speed was 10.0 mm/s, test distance was 6 mm, trigger force was 3 g, data acquisition rate was 400 points per second,room temperature as test temperature, seedless part as testing place , chewiness as detection index.ResultThe validation test showed that chewiness value reflected the strength of brittleness objectively, and its data ranged between 100 and 500 g?sec, consisting with sensory evaluation.ConclusionThe method has the advantages of strong objectivity, high sensitivity and good stability, and can meet the need of quantitative detection of brittleness of pickled cowpea.
  • GU Ya-ping et al
    Food and Fermentation Industries. 2017, 43(12): 214-217. https://doi.org/10.13995/j.cnki.11-1802/ts.014659
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    Formaldehyde in Chinese cabbage was derivated with 2,4-dinitrophenylhydrazine, extracted by acetonitrile and tested by liquid chromatography tandem mass spectrometry. Results showed that the detection limit of formaldehyde in this method was 3.06μg/Kg. When the spiking amout in Chinese cabbage was 1-18mg / Kg, the formaldehyde amout was linearly correlated to peak area. (r2= 0.994). When the adding amount of Formaldehyde was 0.5,1,5 mg/Kg, recoveries were between 102% to 105%, and the relative standard deviations were between 3% and 8% with good stability and precision.
  • CHI Chao et al
    Food and Fermentation Industries. 2017, 43(12): 218-223. https://doi.org/10.13995/j.cnki.11-1802/ts.013902
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    Using the national standards methods, the appearance, grain weight, physical-chemical properties, nutrition components and bio-active compounds of five kinds of red raspberry (Heritage, FertodZamatos, Autumn Bliss, U.S. Raspberry and European red) seeds were analyzed and compared. The results showed that red raspberry seeds were small grain seeds with thousand seed weight of 1.06-1.60 g, water content of 3.23%-8.78%, crude fat content of 12.69%-16.65%, crude protein content of4.64%-8.54%, total sugar content of2.00%-4.26%, reducing sugar contentof 0.97%-2.40%,crude ash contentof0.84%-1.57%,total polyphenols contentof 15.21-18.53 mg/g, flavonoids contentof 8.19-11.50 mg/g, procyanidinscontentof 19.43-31.10mg/g.The contents of vitamin E in different varieties of red raspberry seed were 33.80-52.59 μg/g, but vitamin A, D, K were not detected.Proximate analysis showed that the raspberry seeds were rich in mineral elements, proved to be a good source for minerals Ca,Co,Fe,K,P, and Autumn Bliss seeds had the most abundant mineral element content which wasup to 3520.51 μg/g.The scientific and theoretical basis were provided for red raspberry seeds fully utilized.
  • WANG Rui et al
    Food and Fermentation Industries. 2017, 43(12): 224-231. https://doi.org/10.13995/j.cnki.11-1802/ts.015527
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    Lipase-catalyzed synthesis of fatty acid monoglycerides has obtained a lot of research interests in recent years, since its mild reaction conditions, high selectivity, safe and environmentally friendly. The key to the enzymatic synthesis of fatty acid monoglycerides is the maintenance of enzyme activity and the miscibility of the reaction substrates during the reaction, that is, the selection and regulation of the enzymatic reaction system. The research progress on several enzymatic reaction systems of enzymatic synthesis of fatty acid monoglycerides is mainly introduced, such as organic solvent system, reverse micellar system, solvent-free system, supercritical fluid system and ionic liquid system. Finally, the advantages and disadvantages of aforementioned enzymatic reaction systems were summarized and forecasted.
  • Hao Jun-guang et al
    Food and Fermentation Industries. 2017, 43(12): 232-240. https://doi.org/10.13995/j.cnki.11-1802/ts.015341
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    It is common limiting technological problem and research focus of whole world brewing industry to improve the flavor stability with an aim to keep refreshing character of beer as long as possible to attract consumers in the shelf life of beer. A decrease of the bitterness intensity accompanied by a shift of the taste profile toward harsh and long lingering bitter after is well known phenomena observed during aging of beer. With the help of modern purification technologies, nuclear magnetic resonance spectroscopy and high resolution HPLC MS, the overseas researchers have took some breakthroughs in identifying the compounds responsible for harsh and lingering bitterness and other newly found bitter substances during brewing and storage process of beer as well as their possible transformation routs. In this paper, the highlights of the newly identified bitter compounds originated from hop a- acid were reviewed in detail, especially the formation mechanism, thresholds and elution of newly found tri- and tetra-cyclic molecules, namely main iso-α acids degraded compounds, during brewing and storage were introduced, in order to enlarge the knowledge of beer bitterness of beer brewing peers in China.
  • SUN Lu et al
    Food and Fermentation Industries. 2017, 43(12): 241-244. https://doi.org/10.13995/j.cnki.11-1802/ts.011539
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    Transmission electron microscopy (TEM)  can observe   ultrastructure of less than 0.2μm cell, consequently this technology can conduct the more in-depth research of the fruit and vegetables amples at the molecular level. The paper introduces the working principle and the history of transmission electron microscopy (TEM) technology, and also comprehensively discusses the experimental conditions the best harvest of fruit, the postharvest storage condition and appropriate preservation methods determined by TEM technology. The application prospect of TEM technology used in fresh-keeping and related research is also discussed. TEM technology will get more extensive application in fruits and vegetables preservation.
  • DUAN Zhen et al
    Food and Fermentation Industries. 2017, 43(12): 245-252. https://doi.org/10.13995/j.cnki.11-1802/ts.014637
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     Ultra-high pressure (UHP) or high hydrostatic pressure (HHP) treatment is an emerging food technology, which is an advanced research hotspot among the food high and new technology field at present. This paper elaborates part of the application principles of ultra-high pressure briefly, and then reviews and makes a preliminary outlook the researches and applications of the natural active ingredients extraction from plants by using UHP in recent years at home and abroad.
  • BAI Yu et al
    Food and Fermentation Industries. 2017, 43(12): 253-261. https://doi.org/10.13995/j.cnki.11-1802/ts.014216
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    As a sex hormone estradiol (E2) is the most important form of estrogen in humans and animals, it triggers many of the developments of the reproductive system that begin in puberty, which is involved in a number of physiological processes, including development of the female reproductive structures and secondary sexual characteristics. E2is a typical natural estrogen used extensively in livestock production for growth promotion and performance improvement, widely exist in the environment, and attract much attention due to their high biological estrogen-like effects and carcinogenicity. Therefore, in order to ensure food safety and human physiological health it has very important practical significance to monitor and control the estradiol residues in food source and improve detection technology research and application. In order to provide the references of E2biological activity research and establish convenient and effective detection techniques, this review clarify main residual sources, hazard analysis and detection techniques of food source E2residual.
  • GOU Zhen-qiong et al
    Food and Fermentation Industries. 2017, 43(12): 262-267. https://doi.org/10.13995/j.cnki.11-1802/ts.014120
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    Excessive heavy metals which are harm to human health, many countries have paid more attention to limit standards of heavy metals in food, and there are more and more studies on analysis and detection of heavy metals. The traditional detection methods are mature, high sensitivity. But the general defects of traditional detection methods such as tedious operation, high cost, large-scale equipment and skilled professionals. ELISA method has excellent specificity and sensitivity, and it can be used for rapid detection on site, so develop rapidly. ELISA detection of heavy metals including four key steps: sample pretreatment, synthesis of artificial antigen, preparation of specific antibody, selecting ELISA method. The recent progress of ELISA detection of heavy metals is briefly reviewed in this article. Future development trends and prospects are also proposed in order to provide a relevant gist for detection of heavy metals in food.
  • YUAN Miao-miao et al
    Food and Fermentation Industries. 2017, 43(12): 268-276. https://doi.org/10.13995/j.cnki.11-1802/ts.014924
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    Proper low temperature stress can influence yeast metabolism which is regulated by different mechanisms, and make the yeast react correspondingly and produce different amounts of metabolites, thereby affecting the quality of the wine. Low temperature fermentation can improve wine aromas and organoleptic properties, mainly increasing acetate and ethyl esters and decreasing higher alcohols and volatile acids. With the aim of providing some reference for low temperature fermentation, we mainly review the studies focused on wine aroma components, relative yeast metabolism and gene expression, and analyze the influence of low temperature on wine quality, combined with non-Saccharomyces cerevisiae suitable for low temperature fermentation.
  • GAO Huan et al
    Food and Fermentation Industries. 2017, 43(12): 277-282. https://doi.org/10.13995/j.cnki.11-1802/ts.014456
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    Strophariarugoso-annulata Farlow ex Murrillis one of the edible fungi recommended by the United Nations Food and Agriculture Organization (FAO) to be cultivated in developing countries. It is easy to cultivate, low cost and rich in active ingredients compared with other edible fungi. In this paper, the functional constituents, health functions, progress of processing and preservation technology of Strophariarugoso-annulata were summarized, future research direction of Strophariarugoso-annulata was ahead with the aim to provide a reference for the exploitation and utilization of this resource.
  • HUANG Yan et al
    Food and Fermentation Industries. 2017, 43(12): 283-288. https://doi.org/10.13995/j.cnki.11-1802/ts.015373
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    Nattokinase is a kind of serine protease produced by the fermentation of Bacillus subtilis, which has strong thrombolytic effect.Compared with other thrombolytic agents, nattokinase is easy to be absorbed and has a rapid effect and good safety. Therefore, it is a new thrombolytic agent to be developed.The mechanism and physicochemical properties of nattokinase were summarized,Several methods of isolation and purification of nattokinase were introduced,Including column chromatography, ultrafiltration, extraction and affinity particle adsorption, as well as integrated separation and purification technology,The new method of isolation and purification of nattokinase was prospected to improve the development and application of Nattokinase.