25 November 2017, Volume 43 Issue 11
    

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  • PENG Zhi-fu et all
    Food and Fermentation Industries. 2017, 43(11): 1-8. https://doi.org/10.13995/j.cnki.11-1802/ts.014640
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    The formula of five grains including sorghum, wheat, corn, rice and sticky rice is the particular feature for Wuliangye when compared with other Chinese liquors. In this study, the five grains powder was distilled for two times by water and the distillates were collected separately. The aroma extraction dilution analysis (AEDA) was applied to determine the odor-active compounds in two distillates. The results showed that 2-methylbutanal, hexanal, (E)-2-heptenal, benzaneacetaldehyde, guaiacol, p-cresol, m-cresol, ethyl hexanoate, dimethyl trisulfide and β-damascenone were the most important odor-active compounds (flavor dilution factor (FD)≥81) in 1st distillation, and 2-methypropanal, (E)-2-heptenal, dimethyl trisulfide and guaiacol (FD≥81) were the most important odor-active compounds in the 2nd distillation. The FD values of aldehydes (hexanal, (Z)-2-nonenal), aromatic compounds (benzaldehyde, benzeneacetaldehyde, acetophenone, 4-methylguaiacol, p-cresol and m-cresol), and ethyl hexanoate in 1stdistillation were higher than that in 2nd distillation. The FD factors of guaiacol, 4-ethylphenol, 2,3-butanedione and hexanol in the 2nd distillation were higher than that in the 1st distillation. This study compared the difference of odor-active compounds between the 1st and 2nd distillation of five grains powder and it provided an important database for future researches.
  • WAN Qing-hui et al
    Food and Fermentation Industries. 2017, 43(11): 9-15. https://doi.org/10.13995/j.cnki.11-1802/ts.014780
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    The accumulation is a key process in the production of sesame flavor liquor, and directly affects the subsequent fermentation and the quality of the final sample. Effected by the temperature and oxygen concentration in the accumulation process, at the end of the accumulation, there are significant differences in the amount of yeast and bacteria between the surface and the core, and the saccharification enzymes, starch, acidity, reducing sugar and alcohol between them were also different. In order to investigate the effect of two different accumulation grains on the production of sesame flavor liquor, the actual temperature of fermentation in the pit was simulated by laboratory temperature control, and the surface grains and the core grains were mixed according to different mixing ratio for fermentation. The dynamic changes of physical and chemical indexes in the fermentation process were studied and the final samples were analyzed by GC-MS combined with sensory evaluation. The results showed that there are differences in the physical and chemical indexes of different mixing ratio. The actual production of mixed fermented grains was the closest to the original wine samples of the factory. The middle grains in a larger proportion produced the fermented wine with sesame flavor, it was speculated that the formation of the core grains and the sesame flavor liquor had a great relationship.
  • YUAN Feng-jiao et al
    Food and Fermentation Industries. 2017, 43(11): 16-21. https://doi.org/10.13995/j.cnki.11-1802/ts.015007
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    In orderto construct a genetic engineering phenylpyruvate reductase strain (E. coli/ppr), the expression conditions and bioconversion medium were optimized to increase the production of PLA with whole-cell transformation. A phenylpyruvate reductase-encoding gene, lpppr, was obtained by PCR from the genomic DNA of Lactobacillus plantarum and heterologously expressed in E. coli BL21 (DE3). Taking phenylpyruvic acid as the substrate, single factor and orthogonal experiments was used to optimized the induced expression and whole-cell catalysis conditions. Results showed that after inductionwith 0.5 mmol/L IPTG at 20 ℃ for 8 h, E. coli/ppr displayed the highest LpPPR activity. When phenylpyruvic acid was added up to 25 mmol/L, the product enantiomeric excess percent was over 99.9% and the final yield of PLA could reach 98.38% at 5 h under the obtained optimal catalytic conditionsas follows: temperature 40 ℃, initial pH 6.5 and 20 mmol/L glucose.
  • .YANG Qian et al
    Food and Fermentation Industries. 2017, 43(11): 22-29. https://doi.org/10.13995/j.cnki.11-1802/ts.015008
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    The research on fungal α-amylase mainly focused on the biochemical properties such as the substrate catalytic mechanism, and the relationship between the composition of amino acids residues and the functional properties of this enzyme is unclear. In this paper, three special conserved histidine residues (H120, H200 and H286) that existed in the catalytic domain of Rhizopus oryzae α-amylase were identified by bioinformatics analysis methods of molecular docking and comparative analysis. A series of mutants were constructed base on the above three identified sites using site-directed mutagenesis. The results showed that the structure of the amino acid residue at position 286 has been proved to be related with the optimum temperature, optimum pH, acid resistance and maltose-forming ability of the enzyme. The acid resistance and maltose-forming ability on soluble starch of the mutant H286L were significantly improved. Changes in 120 and 200 amino acid residues exhibited no effects on the optimal temperature, optimum pH and maltose-forming ability, but the combined mutations at both sites of 120 and 200 could improve its acid resistance. The resulted data indicated that the high maltose-forming ability of the enzyme was not directly related to its affinity on maltotriose, which may provide a theoretical reference for the mechanism of high maltose-forming ability and its directed evolution for fungal α-amylase.
  • LI Xiao-min et a
    Food and Fermentation Industries. 2017, 43(11): 30-33. https://doi.org/10.13995/j.cnki.11-1802/ts.014971
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    Filterability is one of the most important quality traits of malt. It is of great practical significance to resolve the problem of filterability defects of Chinese malt. The present study compared that proteomes of Scope and Dan’er malts that with distinct filterability. It was found that arabinoxylanarabinofuranohydrolase and feruloyl esterase involved in the degradation of arabinoxylan were the most different enzymes. In addition, the components analysis of WEAX from Scope and Dan’er malt revealed that WEAX from Dan’er malt contained more ferulic acid. Therefore, we proposed that lack or short of feruloyl esterase in Dan’er malt contributed majorly to its filterability defect. By adding purified recombinant feruloyl esterase in the mashing process using Dan’er malt, the filtration rate of wort was significantly raised, accompanied by the decreased turbidity and viscosity of wort. The feruloyl esterase could remove the ferulic acid in the chain of arabinoxylan effectively and therefore inhibit the formation of ferulic acid dipolymer, which lead to decreased content of poly-arabinoxylan.
  • WU Xiang-yi et al
    Food and Fermentation Industries. 2017, 43(11): 34-38. https://doi.org/10.13995/j.cnki.11-1802/ts.015087
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    To evaluate the antioxidative activity of yak milk casein hydrolysates in vitro, HepG2 cells were treated with H2O2 to establish the oxidative model. The protein carbonyl content and the activity of SOD, GSH-Px and CAT of HepG2 cells pretreated with yak milk casein hydrolysates were measuredto analyze the antioxidative activity of yak milk casein hydrolysates. The results showed that pretreatment with yak milk casein hydrolysates significantly alleviated the increase of protein carbonyl content induced by H2O2 and increased the activity of SOD, CAT and GSH-Px, suggesting the antioxidative capacity of yak milk casein hydrolysatesin vitro. The results suggested that yak milk casein hydrolysates possess protective effects against H2O2-induced oxidative damage in HepG2 cells, whichprovide a basis for the further utilization of yak milk resources and forthe application of the bioactive peptides as antioxidant functional foods.
  • BAN Jia et al
    Food and Fermentation Industries. 2017, 43(11): 39-43. https://doi.org/10.13995/j.cnki.11-1802/ts.015075
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    Docosahexaenic acid production by Schizochytrium sp.using molasses as carbon source was studied: Molasses from different sugar refineries were prepared as carbon source. Molasses sources and molasses proportion in the medium were investigated to evaluate the effect on the resultant dry cell weight, total lipid content and DHA yield. The results indicated that the colloid and excessive metal ions in the cane molasses had the noticeable negative effects on the Schizochytrium sp.growth, the result was depressing even if the proportion of molasses significantly reduced.The application of the cane molasses as a substitute carbon source in the actual production was limited.On this basis the prospect of refined sugar molasses was evaluated.The result was very close between glucose and refined sugar molasses when the refined sugar molasses proportion in the medium is below 80%, refined sugar molasses may partly substitute for glucose as carbon source of Schizochytrium sp.
  • JIAO Cong-rui et al
    Food and Fermentation Industries. 2017, 43(11): 44-50. https://doi.org/10.13995/j.cnki.11-1802/ts.014869
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    Xylanase, an enzyme that randomly cleaves internal β-1,4-xylosidic linkages of xylan backbone, has wide applications in feed, food, pulp and paper, and textile industries. In the present study, gene xynC encoding xylananse from Aspergillus niger CICIM F0510 was cloned and expressed in Pichia pastoris, and the recombinant strain GS115 (pPIC-xynC) was subsequently constructed. At the shake flask level, the activity of the recombinant enzyme XynC was 1.14 U/mL. The optimum temperature and pH of XynC was determined to be 30 ℃ and 2.5, respectively. This enzyme was also stable at 25-40 ℃ or pH 2.0-5.0. K+ slightly enhanced the activity of XynC, whereas Ca2+, Co2+, Mn2+, Zn2+, Cu2+, Fe3+, Sn2+ EDTA and SDS had different inhibitory effects on its activity. Besides, XynC can hydrolyze pentosan to yield short chain xylooligosaccharides with degree of polymerization of 3-6. Such good biochemical properties lay a solid foundation for the applications of XynC in feed and food fields.
  • LIU Wen-ying et al
    Food and Fermentation Industries. 2017, 43(11): 51-57. https://doi.org/10.13995/j.cnki.11-1802/ts.013679
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    The present study was aimed to investigate the effect of wheat oligopeptide and glutamine on non-steroidal anti-inflammatory drugs (NSAIDs) induced gastrointestinal mucosa damage in rats. Method: 70 SD rats were randomly divided into control group, damage group, low, medium and high doses of the wheat peptide group (the doses were 20, 100 and 500 mg/(kg·d), respectively), wheat gluten group and glutamine group (the doses were both 20 mg/(kg·d)). The rats were administered once a day for continuous 30 days. Before sacrificing, NSAIDs were infused into the digestive tract twice. Serum, stomach and small intestine mucosa samples were collected to observe the changes of the indicators of serum cytokine, pathological section of stomach and small intestine, enzyme activity of small intestinal antioxidase and opioid receptor mRNA expression. Results: Wheat oligopeptides administration reduced gastrointestinal mucosa damage, and significantly decreased the level of TNF-alpha in serum. Oxidative stress was significantly increased after NSAID infusion and was reduced by wheat oligopeptides. Wheat oligopeptides increased glutathion peroxidase (GSH-Px) activity in mucous membrane of small intestine. Mu opioid receptor mRNA expression decreased more significantly in wheat oligopeptides treated rats than in the model control group. Conclusion: Non-steroidal anti-inflammatory drugs can induce small intestinal damage in rats and wheat oligopeptides administration may be an effective tool for protecting gastrointestinal tissue against NSAID-induced gastrointestinal damage and oxidative stress. The effect of low doses of the wheat peptide group was the strongest, and stronger than that of glutamine group.
  • LI Bin et al
    Food and Fermentation Industries. 2017, 43(11): 58-64. https://doi.org/10.13995/j.cnki.11-1802/ts.014013
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    The goal of this study is going to shorten the drying time ,improve the quality and reduce the energy consumption of Chinese prickly ash for its vacuum drying. Weibull distribution was adopted to analysis the drying kinetics characteristics of Chinese prickly ash for its Scale parameter α, Shape parameter β, Moisture Effective diffusion coefficients Deff and Geometric factor Rg under the varied drying conditions of vacuum (0.02,0.04,0.06

    Mpa) and temperatures(50,60,70℃). And the quality of dried product was investigated by Weight Grade Method. The result showed that there is a positive correlation between drying time and vacuum(V) and drying temperatures(T); The value of β ranged between 1.3369 and 1.6132, the values are closed to 1 and that means the drying curves obeys the exponential distribution. The values of Deff are 0.715×10-8m2·s-1~2.244×10-8m2·s-1, and the value of Rg under varied drying conditions are closed to 1,and that means the model of Deff have a good fitness. The average value of Ea is 24.36Kj.mol-1. Besides, the color of the product was significant affected by drying temperature,and there was a positive correction between breakage rate and vacuum, drying temperature.
  • TIAN Jun-qing et al
    Food and Fermentation Industries. 2017, 43(11): 65. https://doi.org/10.13995/j.cnki.11-1802/ts.014287
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  • NIU Yi-qing et al
    Food and Fermentation Industries. 2017, 43(11): 75. https://doi.org/10.13995/j.cnki.11-1802/ts.014160
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    The ‘Hass’ avocado had been tested by DMA to study the stress relaxation properties under different storage temperatures. The Maxwell model and kinetics model were also used to build the shelf life prediction model. The results showed that there were no significant changes of stress relaxation property in 7 days when stored under 5℃, while it softened quickly after 5 days at 8℃. The stress relaxation properties changed significantly in 3 days at 25℃. The shelf life prediction models had been built by extracting the equilibrium modulus of elasticity(E0), the main relaxation time(τ1) and the viscous coefficient(η) of samples, respectively. Among them, the model based on the main relaxation time,with the relative prediction error of 8.57%, could predict the shelf life more accurately than the others. This conclusion is expected to be potential applications for shelf life prediction of ‘Hass’ avocado during the cold chain transportation and storage.
  • YAO Hang-hang et al
    Food and Fermentation Industries. 2017, 43(11): 81. https://doi.org/10.13995/j.cnki.11-1802/ts.014288
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    The acid soluble collagen (ASC) of skin of Yunnan bream was extracted, and the amino acids composition, subunit composition, structural characteristics, thermal stability and solubility of which were investigated. Research showed that ASC was rich in Gly, Pro, Ala, low in Tyr, Met, Cys, and the hydroxylation rate of proline was 35.47%; SDS-PAGE revealed that ASC was mainly composed of α1、α2 and β chains, which was in accordance with the structural features of type I collagen; The maximum UV absorption wavelength was at 230 nm; XRD and FTIR showed that the molecule of ASC was closely and regularly arranged and the helical structure of which was maintained; DSC showed the thermal denaturation temperature of ASC were 86.5 and 226.2℃; SEM showed that ASC was uniformly distributed and had a multi-layered micro structure, which was suitable to be used as carrier in food, medicine and cosmetics industry, and base material in biomedicine; the solubility of ASC was relatively high under acidic (pH<4) and low NaCl concentration (<4% (w/v)) conditions. This paper provided a theoretical basis for the comprehensive utilization of skin of Yunnan bream.
  • FAN Zi-wei et al
    Food and Fermentation Industries. 2017, 43(11): 87. https://doi.org/10.13995/j.cnki.11-1802/ts.014430
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    This study compared physicochemical properties of Yunnan soft rice starch with indica rice, japonica rice and glutinous rice starches. The results showed that the four rice starches content were between97.90% and 99.29%.The soft rice of the content of amylose higher than glutinous rice, which belong to low amylose content rice.The four rice starches performed low clarity;compared with the other three rice starches, soft rice starch performed a lower solubility and highest swelling power.The anti-precipitability of soft rice starch behind the glutinous rice starch; the hardness, gumminess and chewiness of soft rice were between japonica rice and glutinous rice, however, springiness and cohesiveness were less than japonica starch; With the increase of shear rate, the apparent viscosity of soft rice starch fall at the fastest rate; the pasting temperature of soft rice starch was in the range of 59.9℃ to 73.9℃, and the retrogradation and cold stability of soft rice starch are slightly worse than that of glutinous rice starch. The enthalpy of soft rice was the lowest in four rice starches. The morphology of soft rice starch are irregular polygonal, and part of the starch surface was hollow, 92.60% of soft rice starch size ranged from 0 to 20μm.
  • QIN Ya-nan et a
    Food and Fermentation Industries. 2017, 43(11): 95. https://doi.org/10.13995/j.cnki.11-1802/ts.013770
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    Grape seed oil was used as the research object, and the oxidative stability of grape seed oil was analyzed after every steps of refining. And adopt the Rancimat method use the induction period as oil stability evaluation index, study effects of antioxidant when they use individually, such as tert butyl hydroquinone (TBHQ) and butylated hydroxyanisole (BHA), ascorbic acid palmitic acid sodium, dl-a- tocopherol, rosemary extract, and antioxidation effect of single composite agent on the oxidative stability of grape seed oil. The results showed that the process of the greatest influence on the acid value of grape seed was the acid removal process, and the most influential technology for the peroxide value was the deodorization process, while the induction period showed a downward trend in the whole refining process.when adding grape antioxidant to the grape seed oil refining, using TBHQ, BHA, ascorbic acid, sodium palmitate and rosemary extract the four antioxidants individually has a better effect, can improve the oxidative stability of grape seed oil significantly, compound four kinds of antioxidants, except TBHQ, the effect is better than composite antioxidants antioxidants when used alone; when the TBHQ:BHA is 3:1 ,the effect is the best.
  • LEI Lin et al
    Food and Fermentation Industries. 2017, 43(11): 101. https://doi.org/10.13995/j.cnki.11-1802/ts.014046
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    Cabbages are playing a vital role in providing minerals, vitamins, antioxidants, and essential amino acids for human diet. However, the data about the effect of maturity on these nutrients in cabbages is not available. Therefore, the present study was performed to examine the compositions of crude protein, crude fat, total soluble sugar, vitamin B1, B2, and C, chlorophylls, total carotenoids, polyphenols, and total flavonoids in cabbages collected at different maturity stages (I~V). As the leaf matured from tender to mature, the contents of crude protein were decreased while crude fat and total soluble sugar were increased. Meanwhile, the contents of vitamin B2 declined while the contents of vitamin B1, vitamin C, chlorophyll a, chlorophyll b, total carotenoids, total polyphenols, total flavonoids, K, Na, Ca, and Mg fluctuated; the contents of Fe、Cu、Mn、Cr、Pb、Hg and Asreached the highest levels at stage Ⅳ. During the ripening of cabbages, the contents of both essential and non-essential amino acids decreased. The comprehensive nutritive value (CNV) was the lowest in cabbages at maturity stage Ⅱ (77), whereas cabbages at maturity stage V had the lowest CNV (60).
  • ZHAO Xiang-ying et al
    Food and Fermentation Industries. 2017, 43(11): 107. https://doi.org/10.13995/j.cnki.11-1802/ts.014898
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    The effects of glucose on xylitol fermentation byCandida tropicalis SFX-Y9 was studied in this paper. Results showed that strains SFX - Y9 with glucose as carbon source to cultivate seeds can shorten training time, improve the concentration of cells, and had little influence upon conversion of xylose.When the fermentation medium was supplemented with a small amount of glucose, it can increase the consumption rate of xylose and shorten the fermentation cycle. Through the control of dissolved oxygen and the glucose fed-batchcan further increase the rate of xylose consumption in 5L fermentor, and the conversion rate of xylose increased by about 15%.Under the condition ofoptimal oxygensupply and glucose as a secondarycarbon source, the production rate of xylitol can reach 5.0 g/L/h,and the rate of conversion can reach more than 80%.The production cost of xylitol by microbial conversion can compete with the chemical hydrogenation, and it has a bright application future.
  • ZHANG Zhe-yuan.et al
    Food and Fermentation Industries. 2017, 43(11): 112. https://doi.org/10.13995/j.cnki.11-1802/ts.014952
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    In this study, goat milk was concentrated by vacuum concentration to study the effect of different concentration of goat milk (12%, 14%, 16% and 18%) on the quality of yogurt.The quality of goat milk yogurt was analyzed by determination of the coagulation characteristics, texture, flavor substances, and viable counts of lactic acid bacteria. Meanwhile, the microstructure of yogurt fermented with different concentration goat milk was observed by scanning electron microscope. The results showed that the fermentation time decreased with the increase of goat milk concentration, accompanied by the increment in acidity and water holding capacity; High concentrations of 2,3-butanedione and acetaldehyde were recorded in yogurt fermented with goat milk with solids of 16%. The viscosity of goat milk yogurt increased by 149.07% with the total solids of goat milk increased.Yogurt fermented with goat milk with solids of 16% displayed with goodtexture characteristics evaluated by the hardness, adhesiveness and gumminess and cohesiveness; The viable counts of lactic acid bacteria in the yogurt was 6.35×109?cfu/mL which is the highest among different milk concentration.The most compact microstructure was measured in yogurt fermented with 16% goat milk with pore diameter of 2.85?μm. In conclusion, when the concentration of goat milk is 16%, goat milk yogurt showed the best quality measured by the flavor and texture characteristics, and viable counts of lactic acid bacteria.


     

  • WANG Yin et al
    Food and Fermentation Industries. 2017, 43(11): 119. https://doi.org/10.13995/j.cnki.11-1802/ts.015149
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    In this search, yogurt was fermented using the goat milk treated with different concentrations of transglutaminase (TG; 1,2,3 u/g protein)at 40℃ for 2h to investigate the effects of TG concentration on yogurt coagulation time, and the acidity, water holding capacity, texture characteristics and microstructure at the end of fermentation and 24 hours’ post-fermentation.The results showed that the coagulation time of the goat milk treated by TG gradually decreased with the increase of TG concentration, but the acidity in curd and fermentation of 24 h had no significant change (p> 0.05) when added different concentrations of TG. The water holding capacity of goat yogurt could be improved significantly(p<0.05) with TG treatment, especially when the concentrationof TG was 2 to 3 u/g protein.The texture analysis showed that hardness and viscosity at the end of fermentation and post-fermentation of 24h ofgoat yogurtincreased significantly with the increase of TG concentration(p>0.05). TG processing had no significant effect on cohesiveness of goat milk at the end of fermentation (p>0.05), while it had significant influence on cohesiveness after fermentation of 24 h (p<0.05). However, TG treatment had little effect on goat yogurt elasticity (p>0.05). By observing the microstructure of acid goat milk, it was found that the protein in goat's milk treated with TG concentration of 2 ~ 3 u/g protein easier to form denser network structure, which facilitated the formation of goat yogurtgels. Overall , the gel properties of goat milk could be improved with the TG treatment in concentration of 2 ~ 3u/g protein.
  • JU Ning et al
    Food and Fermentation Industries. 2017, 43(11): 125. https://doi.org/10.13995/j.cnki.11-1802/ts.014953
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    Studies have shown that non Saccharomyces can produce more aroma components at the pre- fermentation stage of fruit wine, which contributes to the improvement of wine flavor. In order to obtain the non- Saccharomyces suitable for wolfberry wine, 82 strains of yeast were isolated from wolfberry orchard soil, fresh fruit of wolfberry and naturally fermented wolfberry liquid. 3 strains were showed good performance in aroma and tolerance aspects through preliminary screening and tolerance study. By the analysis of 26S rDNA sequence, GF-60 and GF-85were identified as Hanseniaspora uvarum, GB-1 was identified as Torulaspora delbrueckii. Through GC-MS analysis, the wolfberry wine fermented by GF-60 and commercial yeast (ratio 3:1) produced more kinds of  aroma components than which was fermented only by commercial yeast.
  • JIA Ya-nan et al
    Food and Fermentation Industries. 2017, 43(11): 132. https://doi.org/10.13995/j.cnki.11-1802/ts.014921
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    The endophytes separated and purified from mulberry twigs, using morphological observation, physiological and biochemical characteristics and molecular biology method to identify the species.And we have examined the activities of inhibition α-glucosidase andantioxidants of the different extractive phases of fermentation broth ofthe endophytes. The preliminary activities screening shows that three strains of endophyte Pseudomonas fermentation broth hasagood inhibiting activityα-glucosidase and antioxidant ,so we carried out the further study.Two kinds of solvents with different polarity were used, respectively, EAE, NBAE and WAE. And the fermentation liquid was extracted, and the three segment of activity was detected. The EAE(Ethyl Acetate Extract) of strain 4cfermentation broth has a good α-glucosidase activity and ABTS free radical scavenging capacity. IC50 respectively:1.749 mg/mL, 1.376 mg/mL.The EAE of strain 2s fermentation broth the strongest on the DPPH free radical scavenging, the IC50 is 0.048 mg/mL.And the total reducing power was the bestest, and the ascorbic acid equivalent (AEE) is 191.627 mg AAE/g dw. This research is so vital to offer a reference for the use of mulberry endophytic bacteria producefunction food.
  • ZHANG Yang et al
    Food and Fermentation Industries. 2017, 43(11): 140. https://doi.org/10.13995/j.cnki.11-1802/ts.014017
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    In order to study the effect of material composition on the oil absorption of frying sample, the restructured potato chips mixed with different proportions of potato starch, wheat gluten or soy protein isolated and water were used as materials. Different oil fractions were determined by staining method. The structural characteristics of the samples before and after frying were observed by scanning electron microscopy (SEM) and stereomicroscope (SM). It was found that the total oil content and structural oil content decreased as the protein concentration increased. The total oil contents of wheat gluten restructured system were less than soy protein isolate system, for 10.04%-16.69%. The addition of protein affected the surface and internal structure of the restructured potato chips before and after frying, and the oil content decreased with the protein concentration increased.
  • LI Sheng et al
    Food and Fermentation Industries. 2017, 43(11): 146. https://doi.org/10.13995/j.cnki.11-1802/ts.013771
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    To increase the proportion of purple sweet potato flour(PSPF)added in the noodles and improve its nutritional quality,Blending wheat flour with high content of PSPF (HPSPF )(50% wheat flour replacement rate) were used to make noodles. The paper investigated the effects of three different types of improvers, for gluten, Konjac glucomannan(KGM), Sodium stearoyl lactylate(SSL), on the cooking and sensory quality of HPSPF dried noodles. The results obtained with the use of response surface experiment showed that the addition of blending improvers of gluten (3.8%), KGM (0.7%), and SSL(0.06% )could significantly improve the cooking quality,texture profile and sensory quality of HPSP dried noodles, and the mentioned-above qualities of the products were similar to the normal dried noodles.
  • TIAN Xue-zhi et al
    Food and Fermentation Industries. 2017, 43(11): 153. https://doi.org/10.13995/j.cnki.11-1802/ts.014496
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     In order to explore the effect of high hydrostatic pressure on the functional properties of polysaccharide-protein mixture system. Using konjac glucomannan(KGM) and soybean protein isolate(SPI) as raw materials, the emulsifying activity, emulsifying stability, viscosity properties and microstructure of the composite sol were investigated after high hydrostatic pressure processing. The results showed that the functional properties were significantly improved when KGM-SPI composite sol was treated at 400 MPa pressure; And under 400 MPa treatment, the emulsifying activity and emulsifying stability achieved the best at 10 - 15 min(increased by 5.6 - 6.2% and 2.5 - 2.7% ),while the viscosity got the highest value at 15min (increased by 145.3%), compared to the control group. Moreover, the analysis of SEM showed that the composite sol presented a more uniform and dense three-dimensional network structure after 400 MPa treatment, and the interaction between them was enhanced.
  • ZHANG Dong et al
    Food and Fermentation Industries. 2017, 43(11): 159. https://doi.org/10.13995/j.cnki.11-1802/ts.014096
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    To protect people's health, reduce production costs, to guide the development of low-salt meat products,  bacon with different salt dosage was tested for difference of amino acid, moisture content, press loss, thiobarbituric acid (TBA), total soluble protein and sensory quality, (The amount of salt dosage is the quality of the meat’s 2%, 3%, 4%, 5%, 6% ) among them, the amount of salt used was 6% preserved bacon as the control group. The results showed: When bacon adding amount of 4% salt, the highest total amino acid is 45.35 g/100 g; When bacon adding amount of 4% and 5% salt, the moisture content and press loss are the smallest, respectively, about 31% and 32%; When bacon adding amount of 4%, 5% and 6% salt, the TBA are the smallest, respectively, about 0.25 mg/kg; The increase in salt content will promote the degradation of total soluble protein in bacon; The final result of the sensory evaluation showed a maximum weight score of 8.34 when the salt was added at 4% and 5%. Finally showed: Add 4% salt salted bacon better.
  • WANG An-feng et al
    Food and Fermentation Industries. 2017, 43(11): 165. https://doi.org/10.13995/j.cnki.11-1802/ts.014525
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    Response surface method was used to optimize the hydroiysis conditions of Pinctadafucata. The effects of the dose of protease, liquid-material ratio, enzymolysis time and enzymolysis temperature were investigated by degree of hydrolysis. The results showed that the optimized conditions were as follows : 5680 U/g of the amount of enzyme, liquid-material ratio 2.8:1(Vm), 6.5 h ofenzymolysis time, 55 ℃ of the enzymolysis temperature. Under this optimized conditions, the degree of hydrolysis was 44.27±1.23% which was almost same with the theoretical value. The hydrolysis process was simple, reliable, high hydrolysis rate and could beapply to practical production .
  • ZHOU Xin-ya et al
    Food and Fermentation Industries. 2017, 43(11): 172. https://doi.org/10.13995/j.cnki.11-1802/ts.014272
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     This study investigated the effects of different pH conditions on the function of myofibrillar protein in rabbit meat, the correlation between the functional indexes of myofibrillar protein and the mechanism of protein tertiary structure. It was found that, with the pH approaching the isoelectric point range, the aromatic amino acids inside the protein were exposed, the tertiary structure of the protein was unfolded, and the surface hydrophobicity became larger, which resulted in the increase of the hydrophobic interaction between the proteins. Changes in myofibrillar protein structure cause protein solubility to decrease, and accompanied by decreased emulsifying activity and changes in emulsion stability. At the same time, this structural change leads to the change of myofibrillar protein rheological properties. The decrease of pH leads to the decrease of shear stress, and the shear stress changes obviously at the near isoelectric point. With the decrease of pH, the hardness of myogenic protein gel increases, and becomes smaller at near isoelectric point. At the same time, the gel retention of gel is decreasing and the gel whiteness is increasing.
  • WU Peng et al
    Food and Fermentation Industries. 2017, 43(11): 180. https://doi.org/10.13995/j.cnki.11-1802/ts.014373
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    The infrared microwave puffing carrots chips technology research, analyzes the different microwave power and infrared power and heating time on carrots chips of sensory quality and the effect of using the experiment of three factors a[1]nd three levels.Get the best puffing carrot chips by establishing regression equation.At the same time, we analyzed the correlation between the two key evaluation indexes of sensory score and crispness.Results show that to obtain the best quality the parameters are microwave power 1019 w, infrared power 490 w, heating time 36 minutes.Under these circumstances puffing carrots chips of sensory evaluation score the highest, quality is the best. 
  • TANG Chun-hong et a
    Food and Fermentation Industries. 2017, 43(11): 186. https://doi.org/10.13995/j.cnki.11-1802/ts.014201
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Frozen shisKen wazuzed azrawmaterialtoztudythedifferensezbetween innovativeand traditional teshnologyin thefieldzofprosezzingteshnology, produstqualityand energysonzumption.Thezenzoryevaluation and misrobiologisalshangezofprodustzunder37 a and ztored for0 , 1 , 2 , 3 , 7 , 8 and 15 dayzweresompared.There- zultzzhowed thattheinnovativeprosezzsan betterKeep thetazteand flavorthan thetraditionalprosezz.Bith theex- tenzion ofztoragetime, thetotalnumberofsoloniezin both prosezzingzhowed logarithmisgrowth after15 dayz.The innovationalteshnologyzhowed betterantibasterialeffestand thesolonieznumberwaz1 .43 x103 €FU?gwhilethetra- ditionalprosezzingwaz2 .71 x104 €FU?g.Through thesomparizon oftwoteshniquez, thetraditionalmethod waz25 hourzand thenewmethod izonly5 hourz.Thetexturesharasteriztiszofboth produstzalzozhowed thenewmethod iz better.Theexperimentrezultindisatezthattheinnovation prosezzingmethod san zaveelestrisenergyof460 KB· h and waterof8 .92 m3 .
  • TANG Bin et al
    Food and Fermentation Industries. 2017, 43(11): 191. https://doi.org/10.13995/j.cnki.11-1802/ts.014146
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Themoizturesontent, pH, TVB-N, TBA, solonieznumberzand zenzoryazzezzmentwereuzed azin- dexezduringtheztudy.Bhen misrowavepowerdenzitywaz16 B?gand intermittentratio( theratioofmisrowave open timeand slozetimeduringtheperiod ofworK) waz20 z-on ?60 z-off, effestzofdifferentintermittentmisrowave prosezzingtimez(0 ,1 ,2 ) on frezh-Keepingmarinated porK wereztudied.Therezultzzhowed thatthemoretimezof intermittentmisrowave, thebetterinhibitingeffestzon thesontentzofTVB-Nand solonieznumber, buttheworzeon zenzory.Meanwhile, toomanyuzageofintermittentmisrowave, theTBA valueand pH valuewould rizeobviouzly, themoizturesontentwould drop obviouzly.Bazed on theallevaluation indexez, intermittentmisrowaveonsesould ef-

    festivelyinhibitthegrowth ofmisroorganizmzand Keep thequalityofmarinadeporK.

  • YANG Zhuo et al
    Food and Fermentation Industries. 2017, 43(11): 197. https://doi.org/10.13995/j.cnki.11-1802/ts.015065
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    In ordertoidentifytheaerogen whish sauzezthepasKingzoyzausezwelled, twozwelled pasKingzoy zausefrom afastoryin Guangzhou wereanalyzed.UzingMRS medium with 6 % Na€ltosultivatethebasteriain zwelled zoyzause, an anaerobisbasteriawazizolated.Itwazfurtheridentified azLactobacillu?pobusihiibyuzing16 S zequensingand high-throughputzequensing.Yeaztorfunguzsontamination waznotdetested in thezwelled pasKing zoyzausebyuzinghigh-zaltPDAmedium sultivation and P€Ramplifisation with 18 S rRNAprimerz.Therefore, Lac- tobacillu?pobusihiisontamination wazvalidated azthereazon sauzingthepasKingzoyzausezwelled.Rezultofthiz ztudyzhowed thezuperiorityofgenezequensingmethod in detestingtheunKnown and hard-sultivated misroorganizm in food sontamination.


  • NI De-rang et al
    Food and Fermentation Industries. 2017, 43(11): 202. https://doi.org/10.13995/j.cnki.11-1802/ts.015095
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Thezteam ofzteamed zorghum wazuzed azrezearsh objest, and themain flavorsompoundzofzor- ghum zteam wereidentified bydirestimmerzion-zolid phazemisroextrastion ( DI-SPME) soupled with gazshromatog- raphy-olfastorydetestion port&mazzzpestrometris( G€-ODP?MS ) analyziz.Therezultzzhowed thatthearomaof zteamed zorghum forMoutaimaKingwazmainlysompozed ofaromatissompoundz, whish mainlyzhowed thearoma sharasteriztiszofzweetand floral.Amongthem, Phenylasetaldehydehad moresontribution tozweetand floralaroma.

    Meanwhile, 2 -Methoxyphenoland μ-Nonanolastonewerethemain zourseofgrain-liKearoma, and 1 -Ostanol, 2 -Pen- tylfuran and Nonanalgavezorghum zteam thesharasteriztisofgrazz-liKearoma.

  • DENG Xia et al
    Food and Fermentation Industries. 2017, 43(11): 207. https://doi.org/10.13995/j.cnki.11-1802/ts.014646
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    FlavorizoneofthemoztimportantsriteriatoidentifyFenjiu brand.In viewofthezubjestivityoftradi- tionaltazterzand thelimitationzofsurrentinztrumentzzush azzpestrometerand shromatograph, theSurfaseAsouztis BavezNozeTMwazuzed tosollestfingerprintzofzixdifferentbrandzofFenjiu.Bysomparingthedifferenseofthefin- gerprint, sharasteriztispeaKzwereextrasted.Theextrasted featuredatawereanalyzed byprinsipalsomponentanaly- zizand dizsriminantfastoranalyziz, and itzassurasywazverified byBayezdizsriminantfunstion, and probabiliztis neuralnetworK wereutilized toeztablizh theidentifisation modelz.Therezultzzhowed thattheprinsipalsomponenta- nalyzizand dizsriminantfastoranalyzizsould diztinguizh differentbrandzofFenju, and thelattermethod wazbetter than theformermethod.TheprobabiliztisneuralnetworK modelwazeztablizhed and theresognition ratewazup to 100 %.Theztudyzuggezted thattheSurfaseAsouztisBavezNozeTM had good identifisation and slazzifisation ability fordifferentbrandzofFenjiu
  • WANG Jun et al
    Food and Fermentation Industries. 2017, 43(11): 212. https://doi.org/10.13995/j.cnki.11-1802/ts.014063
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Azsreeningmethod of37 food additivezin fruitsanzwazdeveloped byultraperformanseliquid shro- matographysoupled with quadrupole-timeofflight-mazzzpestrometry.Thezamplezwereextrasted with asetonitrile-

    18

    18

    water, and zeparated on an AsquityUPL€BEH € solumn uzingammonium asetatezolution -asetonitrileazthemo-

    bilephazeand meazured byUPL€?Q?TOFmazzwith elestrozprayioninzation in negativemode.Thequalitativeanaly- zezwerebazed on thesharasteriztisfragmentionzofeverysompoundz.Thequantitation limitation were0.02 –0.5

    mg?Kg; thelinearrelationzhip wazgood in thesonsentration rangeof0.5 –50 μg?mL, theresoveriezwere79.5 % –

    103.5 %.Themethod izzimple, zenzitive, assurateand zuitableforzsreeningfood additivezin fruitsanz.

  • CAI Jian
    Food and Fermentation Industries. 2017, 43(11): 220. https://doi.org/10.13995/j.cnki.11-1802/ts.014420
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Ultrahigh performanseliquid shromatographymazzzpestrometryanalyzizmethod wazuzed in theanal- yzizoffolisasid in zportzdrinK.Aftertreated with 10 % ethanolpresipitating, BaterzAtalantizT shromatographis solumn (2 .1 mmx150 mm, 3 μm) wazuzed in zeparation, 0 .1 % formisasid-10 .0 % methanolwazthemobilephaze in gradientelution mode.Thelinearrangeoffolisasid wazin therangeof1 .0 –200 .0 ng?mLwith asorrelation soef-fisientof0 .999 5 .Thedetestion limitwaz0 .4 μg?Kgand thequantitativelimitwaz1 .5 μg?Kg.Theresoveryrate waz91 .0 % –98 .6 %, and therelativeztandard deviation ( RSD) waz2 .6 % –3 .2 %.Themethod haztheadvan- tagezofzimplepretreatment, high zenzitivityand faztdetestion rate.Itizzuitableforfolisasid determination in a widerangeofsonsentration in zportzdrinKz.Theztudyalzoprovidezareferensefornutritionalvalueoffolisasid.
  • XIE Guang-fa et al
    Food and Fermentation Industries. 2017, 43(11): 225. https://doi.org/10.13995/j.cnki.11-1802/ts.015485
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    In resentyearz, thebazisrezearsh of€hinezerisewinehazmadegreatprogrezz, and manynewtesh- nologiezand equipmentzhavebeen applied.Theresentadvansezon thebrewingmisroorganizmz, volatilearomasom- ponentz, funstionalsomponentzofrisewine, and thelateztteshnologiezand equipmentuzed in therisewineinduztry wereintrodused in thizpaper.
  • ZOU Yu-feng et al
    Food and Fermentation Industries. 2017, 43(11): 232. https://doi.org/10.13995/j.cnki.11-1802/ts.014048
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Newedition ofDietaryGuidelinezSsiensereportintroduseznewdemandzofmeatprosezzing, zush az lean meatgel-typemeatprodustz, rezearsh and developmentoflow-zodiumand low-zaturated fatmeatprodustzazwell aztheenhanseofmeatprosezzingzafety.TheKeyteshnisalproblemzexizted in thefieldzofmeatprosezzingand new produstzrezearsh and developmentofgel-typemeatprodustzwerezummarized.Theresentrezearsh ofmeatprosezz- ingwazintrodused, zush azoptimization oftumbling, shoppingand surlingprosezzez, theimprovementofprosezzing propertiezofpale, zoft, and exudative( PSE) meat, thehigh prezzure& ultrazonisazzizted thermalgelformation teshnologyand thegreen gel-manufasturingteshnology.In theend, thefuturetrendzofzsientifisrezearsh ofgel-type meatprodustzprosezzingteshnologywazprozpested.
  • ZHANG Dong et al
    Food and Fermentation Industries. 2017, 43(11): 238. https://doi.org/10.13995/j.cnki.11-1802/ts.014561
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Saltizan importantmaterialin meatprosezzingand itprovidezzaltyflavorofthefood and anti-baste- rioztazizazwellazflavoringenhansing.Buttheamountofzodium in traditional€hinezebeason produstzizveryhigh and arenothealthyifeatingtoomush, itsan lead tohigh blood prezzure, heartdizeazeand othersardiovazsulardiz- eazezorinsreazezrizKzofthozedizeazez.Theroleofzaltin themeatprosezzing, thewayofredusingzodium sontent ofmeatprodustz, and zaltinfluenseon meatqualitywerezummarized.Itprovidezatheoryreferensein ztudyingthe lowzodium healthymeatprodustz.
  • JI Xiao-kai et al
    Food and Fermentation Industries. 2017, 43(11): 244. https://doi.org/10.13995/j.cnki.11-1802/ts.014511
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Elestrisalztimulation ( ES ) izameatagingasseleration teshnologyuzingztimulation on animalsar- sazzez.Thizteshnologyhazan importanteffeston thequalityofpoztmortemmeat.ThemeshanizmzofES asselerating agingprogrezzhavebeen ztudied extenzively.Thetheoryon pH-temperaturewindowand theeffestofES on meatsol- orand waterholdingsapasitybesamenewfosuzezin resentyearz.ThepaperzummarizezthesonseptofoptimalpH- temperaturewindowand reviewztheeffestofES on thealteration ofpH desline, degradation ofsytozKeletalproteinz and myofibrillarultraztrustureofbovinemuzslez.Thizreviewsan provideaguidanseforbeefzlaughteringplantzin applyingES teshnologyproperly.
  • ZHANG Li-hua et al
    Food and Fermentation Industries. 2017, 43(11): 250. https://doi.org/10.13995/j.cnki.11-1802/ts.015179
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Diztillerzgrainzarethemain by-produstzofliquid maKinginduztryfueland ethanolprodustion.Bith thedevelopmentofglobalrezoursezhortagezituation, thevalueofutilization ofgrain ztillagehazbesomeanewre- zearsh hotzpot.Thizztudyprovidezan overviewofgrain ztillagein animalfeed induztry, fuelethanolinduztryfrom sellulozefermentation and funstionalsomponentzexploration.Previouzztudiezindisatezthatthegrain ztillageiza good zourseforfeed duetoitzhigh sontentofprotein, buttheamountsould besonztrained bythehigh sellulozeson- tent.Then, thesonverzion ofsellulozeand redusingitzsontentwillbebenefisialtoinsreazetheamountin feed, and improvethesonverzion rateofethanolfermentation ofsellulozefrom grain ztillage.Moreover, funstionalsomponentz in rezidualgrain ztillagewillbeanotherhighlighttoreuzeit.Therefore, reviewon thereuzeofdiztillergrainzwillpro- videanovelideaand guidanseforitzbetterapplisation in feed induztry, sellulozisethanolsonverzion and funstional somponentzextrastion.
  • FENG Hui-jun et al
    Food and Fermentation Industries. 2017, 43(11): 257. https://doi.org/10.13995/j.cnki.11-1802/ts.015314
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    TheshangeoftaxonomisztatuzofThermoactinomyce?wazreviewed herein.Theeffestzofdifferenttax- onomissharasteriztiszon theslazzifisation ofThermoactinomyce?werezummarized.€lazzifisation ztatuzand funstional rezearsh ofThermoactinomyce?werealzobrieflyzummarized.
  • LIU Yan-yun et al
    Food and Fermentation Industries. 2017, 43(11): 262. https://doi.org/10.13995/j.cnki.11-1802/ts.015027
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Jiangzhuiizatraditionalnaturalfermentation food in Northwezt€hina.Ithazalonghiztory, rish nu- trition health sareeffisasy, and uniquelosalflavor.In thizpaper, thefermentation prosezzofJiangzhui, itzmisrobial floradiverzity, thephyzisaland shemisalindisatorzofitzprodustwerereviewed.Itaimed toprovidethebazizforthe induztrialprodustion and theoretisalrezearsh ofJiangzhui, and providereferenseforthedevelopmentofJiangzhuiin- duztryshain.
  • SONG Yuan-de
    Food and Fermentation Industries. 2017, 43(11): 268. https://doi.org/10.13995/j.cnki.11-1802/ts.013594
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Artisle84 ofFood SafetyLawofthePeople′zRepublisof€hinarequirezfood inzpestion inztitutionzto eztablizh thequalifisation authentisation and inzpestion sriterion bythefood and drugzupervizion and adminiztration departmentundertheState€ounsil.TheFood and DrugAdminiztration hazformulated theQualifisation Authentisa- tion €onditionzofFood Inzpestion Inztitutionz” ( Hereinafterreferred toazQualifisation Authentisation €onditionz” ) whish willbeuzed azzupplementaryrequirementzfortheprofezzionalassreditation offood inzpestion inztitutionz. Thizartisleelaboratezthezyztemeztablizhment, themain sontentand differensezbetween theold and thenewverzion ofQualifisation Authentisation €onditionz.Thenew verzion putzforward zpesifisrequirementzin theazpestzof managementzyztem, inzpestion sapabilitiez, perzonneland otherzuggeztionzon edusatingtheregulation.