The effects of Astragalus on the content of active ingredients of mycelia and fruiting bodies of Lentinus edodes were studied. The contents of total polysaccharides, total phenolic acids, total flavonoids and total saponins were determined by ultraviolet spectrophotometry. The content of calycosin-7-O-β-D-glucoside and fomononetin-7-O-glucoside were determined by high performance liquid chromatography. The results showed that the contents of total polysaccharides, total phenolic acids, total flavonoids, total saponins, calycosin-7-O-β-D-
glucoside and fomononetin-7-O-glucoside in Astragalus waste were 46.71 mg/g, 2.62 mg/g, 10.24 mg/g, 10.03 mg/g, 13.56 μg/g, 3.78 μg/g. Compared with Astragalus, the multiple of the components was 3.91, 1.89, 1.77, 2.05, 5.21, 5.24, respectively. The aqueous extract of Astragalus can inhibit the growth of mycelia of Lentinus edodes. Inhibition rate increased with the higher concentration of aqueous extract of Astragalus. When the maximum concentration of aqueous extract of Astragalus membranaceus was 0.5 g/100 mL, Lentinus edodes mycelium’s growth is not significantly inhibited. In Lentinus edodes mycelium which is added 0.5 g/100mL of aqueous extract of Astragalus, the contents of total polysaccharides, total phenols, total saponins and total saponins were 52.52 mg/g, 0.97 mg/g, 0.35 mg/g, 11.11 mg/g, the conversion rates were 1.08 % , 2.20 %, 0.17 % and 13.56 %. In Lentinus edodes fruiting bodies which is added Astragalus waste, the contents of polysaccharides, total phenolic acids, total flavonoids and total saponins were 60.48 mg/g, 1.16 mg/g, 0.68 mg/g, 3.56 mg/g, the conversion rates were 29.43 %, 6.26 %, 0.63 % and 9.09 % respectively. In the experimental group, the mycelium and the fruiting bodies of Lentinus edodes were found calycosin-7-O-β-D-glucoside, the contents were 1.09 μg/g and 0.86 μg/g respectively.Astilbin is a dihydroflavonol that is widely distributed in many foods and plants. Our previous study showed astilbin could significantly decrease the body weight gain and intraperitoneal adipose tissue weight, as well as triglyceride content in serum. In this paper, we studied the inhibitory activity of astilbin on pancreatic lipase. The results showed that astilbin had inhibitory effect on pancreatic lipase with IC50 of 105 μM and maximum inhibition rate of 56.3%. The inhibitory effect increased with the interaction time between astilbin and pancreatic lipase. Enzyme kinetics study showed that astilbin is a noncompetitive inhibitor of pancreatic lipase, which does not change its affinity with the substrate, but decrease its maximum reaction rate. The fluorescence spectra showed that astilbin could quench the endogenous fluorescence of pancreatic lipase, and make its maximum emission wavelength red shift, their binding constant (lgKa) was 5.50 and the binding-site number was 1.17.
