25 August 2018, Volume 44 Issue 8
    

  • Select all
    |
  • PENG Feng et al
    Food and Fermentation Industries. 2018, 44(8): 1-7. https://doi.org/10.13995/j.cnki.11-1802/ts.016910
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    For researching the expression model of B-type natriuretic peptide (BNP) in Corynebacterium glutamicum, a fast protein expression system was constructed using GFP as a model protein. The results showed that constitutive intracellular patterns were the best way for BNP expression compared with inducible intracellular, constitutive secretory and inducible secretory patterns. The BNP was purified and its production was about 235.5 mg/L with high purity (>90%). Conclusively, a fast protein expression system with different expression patterns was constructed in C. glutamicum, and BNP was successfully expressed using this system. This will greatly facilitate the application of this important platform organism.
  • CUI Juan et al
    Food and Fermentation Industries. 2018, 44(8): 8-13. https://doi.org/10.13995/j.cnki.11-1802/ts.016879
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Endo-β-N-acetylglucosaminidase (ENGase) belonging to the glycoside hydrolase family 85 (GH85) is a series of dual-functional enzymes that not only hydrolyze the glycosidic bonds of the N,N′-diacetylchitobiose moieties of N-glycan cores but also transfer chemically synthesized-homogeneous glycans onto glycoproteins. This unique transglycosylation activity enables them to modify proteins with a defined N-glycan structure. In this study, we identified ENGase homolog in Trypanosoma brucei genome by database search. The gene encoding ENGase was cloned into an expression vector with an N-terminal Nus tag, and expressed in Escherichia coli cells. The recombinant ENGase (Nus-Endo Tb) exhibited hydrolytic activity for high-mannose, bi-antennary N-linked oligosaccharides. Such hydrolytic activity can be applied to hydrolyze N-glycans attached to RNase B and human transferrin. Moreover, Nus-Endo Tb also can transfer a sialobiantennary type complex oligosaccharide onto MU-GlcNA, which indicated its transglycosylation activity.
  • WANG Zhen-dong et al
    Food and Fermentation Industries. 2018, 44(8): 14-19. https://doi.org/10.13995/j.cnki.11-1802/ts.016962
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Maltooligosyltrehalose trehalohydrolase (MTHase), which is derived from the Sulfolobus acidocaldarius ATCC 33909, is a key enzyme in the couple enzymatic production of trehalose. The mutant strain L202P/L218D/Y323G, which has a 1.9-fold increase in protein expression, was used for the determination of enzymatic properties and trehalose conversion. The optimum temperature,optimum pH,pH stability and 60 ℃ half-life of mutant strain L202P/L218D/Y323G SaMTHase were determined. The results showed that there was no significant change in the enzymatic properties of the mutant strain. The kinetic parameters of the mutant strain L202P/L218D/Y323G SaMTHase were determined using maltotetraosyl trehalose as substrate. Using Maltodextrin (DE 5-7) as substrate, maltooligosyl trehalose synthase (MTSase) was mixed with the mutant L202P/L218D/Y323G SaMTHase at different ratios. The trehalose conversion results showed that trehalose conversion ratio was up to 76.0%.
  • YU Long et al
    Food and Fermentation Industries. 2018, 44(8): 20-26. https://doi.org/10.13995/j.cnki.11-1802/ts.016966
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    As a staple amino acid product, L-aspartic acid has been widely used in food, medicine and chemical industries. Six different maleate cis-trans isomerase and L-aspartic acid amino lyase co-expression systems have been built in E. coli BL21 (DE3) ΔfumAC in this study. By comparing their co-expression results and catalytic efficiency, the optimal co-expression system E. coli BL21 (DE3) ΔfumAC/pRSFDuet-1-maiA-aspA was obtained. 3.2 mol/L of maleic acid was completely transformed into L-aspartic acid ammonium in 390 minute with OD600 of 8. The concentration of L-aspartic acid ammonium reached 3.14 mol/L, conversion rate was above 98%, and the intermediate fumaric acid had almost no accumulation overall the reaction progress. Furthermore, to solve the problem that is difficult to recycle cells in high concentration of viscous reaction liquid, cell immobilization was studied. After 8 recycles of the immobilized cells, the relative enzyme activity was still 81%. The result suggested that the utilization rate of cells were greatly improved and the production cost reduced, which laid the foundation for the future industrial production of L-aspartic acid.
  • WANG Yun-long et al
    Food and Fermentation Industries. 2018, 44(8): 27-33. https://doi.org/10.13995/j.cnki.11-1802/ts.017175
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    In order to improve the yield of L-asparaginase (EC 3.5.1.1, L-ASNase) produced by recombinant Bacillus subtilis, the fermentation medium was optimized by using artificial neural network algorithm. First, the significant factors were screened through single factor experiment and Plackett-Burman experiment. Then the central composite experiment was used to establish the experimental data samples. Finally, a neural network model was established by using JMP10.0 to optimize the composition of fermentation medium. After optimization, the best medium was obtained as follows: sucrose 65 g/L, yeast peptone 28 g/L, corn syrup 11 g/L, KH2PO4 11.5 g/L, NaCl 3.3 g/L, ammonium sulfate 4 g/L, K2HPO4·3H2O 22.5 g/L, MgSO4·7H2O 1 g/L, and L-asparagine 2 g/L. L-ASNase activity reached 515.6 U/mL in the optimal culture medium, which was 90.9% higher than that before optimization. These results presented a basic data for jar fermentation optimization of L-ASNase.
  • LIU Ying et al
    Food and Fermentation Industries. 2018, 44(8): 34-40. https://doi.org/10.13995/j.cnki.11-1802/ts.016987
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    In order to delay the sporulation and improve the growth efficiency of Clostridium butyricum, the effects of guanine (0.2 g/L) and Ile (0.5 g/L) on the growth and acids metabolism of C. butyricum in 20 L bioreactor were investigated. The results showed that the maximum biomass with the addition of guanine or Ile reached 5.31×108 CFU/mL and 4.65×108 CFU/mL, respectively, which was increased by 22.91% and 7.64% compared with the control (4.32×108 CFU/mL). The coefficient of butyric acid to maximum biomass (Yp/x) was decreased by 48.64% and 40.54% compared with the control (0.37 g/g), respectively. Gunanine and/or Ile addition could reduce the specific butyrate productivity, enhance the growth efficiency and delay the sporulation of C. butyricum. The biomass reached 5.4×108 CFU/mL when guanine (0.2 g/L) and Ile (0.5 g/L) were simultaneously added to the fermentation medium, which was 25% higher than that of the control. This research provides new methods for industrial fermentation of C. butyricum.
  • SU Xian-feng et al
    Food and Fermentation Industries. 2018, 44(8): 41-48. https://doi.org/10.13995/j.cnki.11-1802/ts.017057
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    To solve the problem of fermentation wastewater in citric acid industry, the laboratory had proposed integrated citric acid-methane production process. Previous research indicated that scale formation was observed in anaerobic digestion effluent with electrodialysis treatment, and air stripping consumed energy to heat anaerobic digestion effluent to 55 ℃. In order to solve the above problems, lime softening-ultrafiltration technology was introduced to eliminate scale and replace air stripping. The optimal lime dose was 2.5 g/L. The optimal operating pressure and crossflow velocity respectively was 0.20 MPa and 4.023 m/s. The Langelier saturation index (LSI) of concentrate was less than 0 and the scale was removed. The citric acid production with the dilute of electrodialysis (134.67±4.72 g/L) was comparable to production with freshwater.
  • DENG Wei-qin et al
    Food and Fermentation Industries. 2018, 44(8): 49-56. https://doi.org/10.13995/j.cnki.11-1802/ts.016817
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    In order to find out the dynamic changes of microbial diversity, physical and chemical indicators in the fermented Paocai, the microorganisms in the Paocai brine of each generation were isolated and identified by culturing method and the total acid, pH, nitrite, organic acids and other physical and chemical indicators were detected. The results showed that lactic acid bacterial and yeast were the dominant bacteria and fungi in the Paocai brine respectively. L.pentosus, L. plantarum, L. brevis and L. citreum were main lactic acid. K. exigua, P. membranifaciens, C. tropicalis and P. kudriavzevii were main yeast. The nitrite content of multi-generated fermented Paocai and brine decreased gradually, reaching 0.63-0.81 mg/kg in the later period, which was much lower than 20 mg/kg of nitrite in Chinese Paocai products. The total acid in the multi-generational fermented Paocai brine showed an increased trend from 0.58 g/dL to 0.9 g/dL in the last generation. There are abundant organic acids, organic acid salts in Paocai brine. Organic acids in Paocai brine mainly include tartaric acid, succinic acid, acetic acid and lactic acid. Lactic acid and acetic acid increased and accumulated with the increase of fermentation algebra. Finally they remained basically stable in the later period. The results revealed that the microorganisms and physicochemical basically remained steady in the later period in the multi-generational fermented paocai brine. The results of this study can provide data reference to guide the traditional Paocai industrialization.
  • ZHU Guang-su et al
    Food and Fermentation Industries. 2018, 44(8): 57-64. https://doi.org/10.13995/j.cnki.11-1802/ts.016993
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    To determine whether the probiotic strain, Bifidobacterium adolescentis Z25 and Lactobacillus reuteri 138-1, isolated from human feces with the function of metabolic syndrome alleviation are edible for subsequent product development, safety of these two strains were assessed. Acute toxicity test, 28-day feeding toxicity test, teratogenicity test and bacterial translocation test were performed. The general appearances, body weight, organ weight, biochemical and hematological indexes of blood, bacterial translocation, histopathological indexes, teratology index of pregnant rats and offsprings were investigated. Except for reduced appetite and body weight gain, no major changes were seen in organ index after probiotics treatment. Except for occasionally non-probiotics-related lung damage, no other tissue lesions and bacterial translocation were detected. No teratogenicity and mutagenicity to the pregnant rat and embryo rat were observed. B. adolescentis Z25 and L. reuteri 138-1 are safe strain without toxicity and side effects.
  • WANG Ying et al
    Food and Fermentation Industries. 2018, 44(8): 65-70. https://doi.org/10.13995/j.cnki.11-1802/ts.017045
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    In this experiment, 10 strains of Streptococcus thermophilus strain from Shenzhou-11 return spacecraft was isolated and purified. The growth curve, titration acidity, viscosity and extracellular polysaccharide content of wild strain G2 and test strains were measured, and scanning electron microscopy (SEM) and transmission electron microscopy (TEM) tests were performed. The results showed that OD600 value of two strains B2-1 and B2-4 (2.073±0.15, 2.06±0.11), titration acidity (106±0.23) °T, (98.5±0.25)°T, viscosity (3.27±0.04) Pa·s, (2.94±0.01)Pa·s, and Extracellular polysaccharides (409.35±1.38) mg/L, (398.80±5.38) mg/L were all higher or significantly higher than those of wild strain G2 with OD600 of (2.051±0.11 ), Titration acidity of (92±0.16) °T, viscosity of (2.66±0.07 )Pa·s and extracellular polysaccharide content of (385.11±3.074) mg/L. Finally, two strains of Streptococcus thermophilus with excellent characters of B2-1 and B2-4 were obtained. Therefore, space mutation breeding may change the Streptococcus thermophilus fermentation performance.
  • SHI Xiao-meng et al
    Food and Fermentation Industries. 2018, 44(8): 71-77. https://doi.org/10.13995/j.cnki.11-1802/ts.015076
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Lactobacillus plantarum CICC 6238 was screened out for γ-aminobutyric acid (GABA) production from 38 lactic acid bacteria deposited in the China Center of Industrial Culture Collection (CICC). The high-yield GABA strains were obtained by ultraviolet mutagenesis,and its production of GABA was (1.196±0.033) g/L,which was 2.33 times as much as original strain. CICC 6238 was studied extensively for its high tolerance to simulated gastric, intestine juices and bile salts with commercial probiotic strains (Lactobacillus acidophilus NCFM and Lactobacillus rhamnosus HN001) as positive control. After incubation in bile salt environment, the survival rate of CICC 6238 was (36.90±1.63)% significantly higher than NCFM [(15.16±1.73)%]. After digestion in artificial gastric juice for 3 hours, the survival rate of CICC6238 was (84.05±1.87)%, which was remarkably greater than that of HN001 [(61.97±2.45)%]. After incubation in artificial intestine juices for 6 hours, the survival rate of CICC 6238 was (61.50±1.94)%, which was slightly lower than that of HN001 [(77.44±3.08)%] and NCFM [(79.42±2.08)%]. And CICC 6238 also could inhibit the growth of four kinds of intestinal tract pathogenic bacteria, especially Shigella flexneri with the inhibition zone reaching to (28.31±0.27) mm. The results suggested that CICC 6238 has high-yield of GABA and potential probiotic properties, which showed good potential for application in functional foods and health-related products.
  • BAI Jie et al
    Food and Fermentation Industries. 2018, 44(8): 78-85. https://doi.org/10.13995/j.cnki.11-1802/ts.016032
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    As a kind of potential antibiotic alternatives, antimicrobial peptides possesses great development potential. In this paper, an effective method for extracting and purifying antimicrobial peptide from a Bacillus subtilis B3 strain isolated from the saline soil of Gansu was established to provide a reference for the preparation of antimicrobial peptides and analysis of product. In this experiment, a chromatography system composed of cation exchange chromatography, hydrophobic chromatography and size exclusion chromatography unit was used to extract antibacterial substance from the fermentation broth of Bacillus subtilis B3. The antibacterial experiments were carried out to evaluate the effect of step-by-step chromatography, and then the antibacterial active peptide was further purified by RP-HPLC and analyzed by ABI-4800 chromatography to obtain the molecular weight and structure information. The antimicrobial active substance isolated by tandem chromatography was a kind of small proline-rich antimicrobial peptide. The amino acid sequence was Pro-Tyr-Ile-Pro-Gln-Pro-Arg-Pro-Pro-His-Pro-Arg-Leu, its molecular weight was 1 567.86 Da, and its isoelectric point was 10.84. By NCBI database comparison, it was found to be an unreported peptide. It was proved that this peptide had good antibacterial activity against Escherichia coli and Salmonella enterica subsp. eterica, and the minimum inhibitory concentration was 0.020 312 μg/mL. Meanwhile, the antibacterial peptide exhibited strong heat resistance, acid and alkali resistance and light stability. We have obtained a novel peptide with antibacterial activity against Escherichia coli and Salmonella enterica subsp. eterica from Bacillus subtilis B3 strain.
  • LI Zhen et al
    Food and Fermentation Industries. 2018, 44(8): 86-92. https://doi.org/10.13995/j.cnki.11-1802/ts.016176
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Malve nut gum (formed by cortex of Sterculia lychnophora expansion in the water) application in noodle products was studied. The effects of malva nut gum amounts at 0%、0.2%、0.4%、0.6%、0.8%、1.0% (w/w) on thermal mechanical properties, pasting properties, and texture, cooking loss, color of fresh noodles were studied. The results showed that appropriate amount of malva nut gum could enhance the thermal stability of starch, and inhibit starch staling. The malva nut gum had a significant influence on increasing water absorption and reducing cooking loss of noodles. Smooth sensory feeling of noodles was also improved. Color analysis showed that malva nut gum reduced the noodles brightness and became coffee color. In summary, the best overall quality of fresh noodles were obtained by adding 0.4%-0.6% malva nut gum.
  • ZHU Pan-hong et al
    Food and Fermentation Industries. 2018, 44(8): 93-98. https://doi.org/10.13995/j.cnki.11-1802/ts.016129
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Myosin was extracted from tilapia muscle, the changes of turbidity, solubility, surface hydrophobicity and sulfhydryl content of myosin under different salt concentration (1, 50,150, 600 mmol/L NaCl) were investigated. The heat-induced myosin gel was prepared and the formation and mechanism of thermal gel was discussed. Results showed that with the increasing of ionic strength, solubility of myosin gradually increased and turbidity decreased with the increasing of the surface hydrophobicity and -SH content. The solubility showed a significant downward (p<0.05), while the surface hydrophobicity was increased (p<0.05) and sulfhydryl content was decreased with heat treatment from 20 to 80 ℃ at 1 ℃/min., which indicated thermal denaturation and aggregation of myosin happened. Heat-induced dynamic elastic modulus of myosin dispersion increased with the increasing of salt concentration. Main force of three-dimensional network structure stabilized gel structure was hydrophobic interaction, disulfide bonds and non-disulfide covalent bond. Myosin gelation network structure is more stable under high ionic strength condition.
  • LIANG Di et al
    Food and Fermentation Industries. 2018, 44(8): 99-106. https://doi.org/10.13995/j.cnki.11-1802/ts.016142
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    The edible coating was made up of 5% apple pectin solution as the substrate and different concentration of apple polyphenol as the additive. The effects of the amount of apple polyphenol on the rheological properties and antioxidant activities of the edible coating were investigated. The results suggested that the control (pectin solution) and the mixture liquids all exhibited a shear-thinning behavior, indicating that they are typically non-Newtonian fluids. The increase of polyphenol addition amounts caused the significant increase of viscosity of the liquids: when the polyphenol concentration increased to 10 g/L, the viscosity reached to approximately 220 Pa, significantly higher than the control (108 Pa). Moreover, as the polyphenol addition increased, the antioxidant activities of the mixture liquids including the inhibition capacity against lipid oxidation, DPPH scavenging capacity and total reducing capability were significantly enhanced. At 10 g/L of the polyphenol concentration, the prepared mixture liquid had a higher antioxidant activity than that of BHT. However, the continuous increase of the polyphenol concentration did not lead to significant enhancement of the viscosity and antioxidant activity of the mixture liquid. In conclusion, a high viscous mixture liquid can be obtained by adding 10 g/L polyphenol into 5% pectin solution, and it can be used as a potential edible coating material in food preservation due to its good antioxidant activities.
  • TAN Jiu-ming et al
    Food and Fermentation Industries. 2018, 44(8): 107-113. https://doi.org/10.13995/j.cnki.11-1802/ts.016007
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    The effect of sodium alginate amount on the stability of whey protein solution was studied. The result showed that with the increase of sodium alginate amount, the ζ-potential of the whey protein-based solution decreased gradually, and the particle size and turbidity of the whey protein-based solution increased first and then decreased, and the microstructure of microscopy and fluorescence microscopy changed from disorder to uniform order. When pH value is close to the innate point of the whey protein isolate, the stability of the whey protein-based solution was better than that of whey protein solution. When NaCl concentration increased to 0.20 mol/L, the stability of the whey protein-based solution was weakened obviously. In the microscope image, the complexes flocculate and the fluorescence appears to accumulate. When the mass ratio of whey protein and sodium alginate was 3∶2.5, the stability of the whey protein-based solution was the best. The results showed that sodium alginate improved the stability of the whey protein solution significantly, which can expand the use of whey protein isolate and provide technical reference for the application of whey protein-based solution in food industries.
  • LI Cong-hu
    Food and Fermentation Industries. 2018, 44(8): 114-119. https://doi.org/10.13995/j.cnki.11-1802/ts.015495
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    The functional properties including water binding capacity, foam capacity and emulsion capacity for catfish skin gelatin were investigated in this paper. The results showed that the water binding capacity of gelatin firstly increased and then decreased with the increase of ionic strength. Due to the macromolecule and the hydrophilicity of gelatin, the foam capacity was worse than that of tween 80. The foam capacity of gelatin increased with the increase of concentration, while decreased with the time. Meanwhile, the gelatin emulsion was O/W type, and the emulsion capacity was the same with tween 80 according to the results of micro-morphology. With the increase of concentration, the emulsion activity index (EAI) of gelatin increased from 29.32 to 39.33 m2/g, while emulsion stability index (ESI) decreased to 19.27 min. Salting-in effect benefits the increase of EAI in the gelatin, while salting-out effect and electrostatic interaction decreased ESI. Meanwhile, both EAI and ESI of gelatin decreased to the minimum value when pH was close to pI. Due to desorption and aggregation, EAI and ESI of gelatin decreased gradually when the temperature exceeding 30 ℃. The functional properties of catfish skin gelatin could provide the theoretical guidance for the application in food industry.
  • ZHONG Li et al
    Food and Fermentation Industries. 2018, 44(8): 120-126. https://doi.org/10.13995/j.cnki.11-1802/ts.015108
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    To select good Chinese herbal medicine extracts as chilled pork preservative. We extracted 15 Chinese herbal medicines (such as Artemisia argyi, Hedyotis diffusa and Eriobotrya japonica) effective component through ultra-wave soaking. With potassium sorbate as control group, we coped with the chilled pork by coating preservation. Next to, we counted the changes of colony number (in 6, 9, 12, 15 days) and determined the total antioxidant capacity of different extracts. Finally, we analyzed 15 Chinese herbal medicine extracts effective components by GC-MS. The different antioxidant capacity and inhibitory effect of different extracts of Chinese herbal medicines. There is no correlation both the aforementioned. According to the GC-MS analysis, we can found that most of Chinese herbal medicine extracts containing organic acids, alcohols, furan, chemicals, etc. Comprehensive consideration antibacterial effect and oxidation resistance, we preliminary screening the Eriobotrya japonica extract can be used as chilled pork antistaling agent priority. Nevertheless, Lepidium apetalum and Salvia miltiorrhiza extracts can be used as a short-term antistaling agent for further utilization.
  • PENG Yuan-yuan
    Food and Fermentation Industries. 2018, 44(8): 127-134.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    The results showed that 0, 0.3, 0.6, 0.9, 1.2, 1.5 g/100mL of low methoxyl pectin were added to sodium caseinate in the presence of sodium caseinate as the raw material. Effects of low methoxyl pectin on rheological properties and microstructure of sodium caseinate solution were studied. The results showed that low methoxyl pectin had a great influence on the rheological properties of sodium caseinate solution. With the increase of the amount of low methoxyl pectin, the fluid index n of the composite system decreases and the consistency coefficient K increases gradually, which shows the characteristics of the typical pseudoplastic fluid. The viscoelasticity of the system was the best at 15 g/100mL of the pectin. The Zeta potential and particle size showed that the average particle size of the pectin was increased with the increase of the electrostatic attraction between polysaccharide and protein. Microstructure observation revealed that with the increase of pectin concentration, the structure of the protein system changed from globular to branched network structure. When the pectin content reached 15 g/100mL, the solidification characteristics of the system were the most obvious.
  • ZHANG Min-jia et al
    Food and Fermentation Industries. 2018, 44(8): 135-140. https://doi.org/10.13995/j.cnki.11-1802/ts.015585
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    The aim of this study was to investigate the immuno-regulatory effects of pea peptides on immunosuppressive mice. Mice injected with cyclophosphamide(CTX)was the model group and normal saline was the control group. Different dosages of pea peptide(0.4 g/(kg·bw),0.8 g/(kg·bw),1.6 g/(kg·bw)were given to low,middle and high groups for 15 days. The body weight, spleen, and thymus weight of mice,morphologic variations of immune organs,white blood cell count,T lymphocyte subsets of spleen,immunoglobulin content,cytokine levels, bone marrow nucleated cell count and DNA content were tested. The morphological changes of spleen and thymus were observed under microscope in the model group. The number of white blood cell,proportion of CD3+,CD4+,CD8+ T lymphocyte,concentration of immunoglobulin IgG and IgM, and nucleated cell counts and DNA density in bone marrow were significantly lower than the control group (p<0.01). The concentration of IFN-γ was significantly lower than that of the control group (p<0.05). Compared with the model group, pea peptide groups showed different degrees of improvement in the above indicators. Pea peptide can improve the immune function of immunosuppressive mice induced by CTX.
  • HOU Yan-jie et al
    Food and Fermentation Industries. 2018, 44(8): 141-148. https://doi.org/10.13995/j.cnki.11-1802/ts.015799
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Based on the Xin-Jiang red-fleshed apple, the drying characteristics of apple slices were studied in terms of hot-air temperature, slice thickness and hot-air velocity. Origin 8.0 software was used to analyze data and optimize the mathematical model of red-fresh apple slices drying. The result showed that there factors affected the drying characteristics, and hot-air temperature had the greatest impact. Increasing hot-air temperature and hot-air velocity, or decreasing the thickness of slice, the drying rate(DR) of red-fleshed apple slices increased. When hot-air temperature was 80 ℃, the slice thickness was 2mm, hot-air velocity was 1.5 m/s, drying rate of red-fleshed apple slices was the highest. It was showed that all 6 drying models could describe the moisture changes of red-fleshed apple slices during hot-air and thin-layer drying. Page model had the highest R2, the lowest root mean square error (RMSE) and χ2. Therefore, it was the optimum model.
  • MA Yu et al
    Food and Fermentation Industries. 2018, 44(8): 149-156. https://doi.org/10.13995/j.cnki.11-1802/ts.016792
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    An integrated fermentation-separation process for the production of ε-poly-L-lysine (ε-PL) was investigated. Through the screening of several aqueous two-phase systems, the extraction of ε -PL by ethanol/ammonium sulfate aqueous two-phase system was investigated in detail. The results indicated that the highest partition coefficient (18.33) and recovery (98.02%) of ε-PL were obtained with an aqueous two-phase system composed of 20% (w/w) ethanol and 20% (w/w) ammonium sulfate, pH9.5. The effect of multi-stage extraction was investigated. It was found that the effect of extraction for three times was the best, the removal rate of protein and pigment were 85.66% and 60.00%, respectively, and the yield of ε -PL was 96.15%. Finally, the top phase after three times of extraction was desalted and concentrated by ultrafiltration membrane, and the yield and purity of the product reached 68.28% and 92.39%, respectively.
  • WANG Yu-xia et al
    Food and Fermentation Industries. 2018, 44(8): 157-165. https://doi.org/10.13995/j.cnki.11-1802/ts.016657
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    The influences of sugar and rose concentrations on quality of citrus wines were studied. The changes of total sugar and acid during the fermentation were assayed and the differences of flavonoid, polyphenol, tannin, and antioxidant activity between different wines were compared. All htese will provide theoretical basis for the abundant production of citrus-rose wine. The results showed that higher rose amount promoted the sugar consumption and total acid production during fermentation. The contents of polyphenol and tannin coupled with antioxidant activity were also significantly increased with the addition of rose. The amount of rose showed positive correlation with the contents of flavonoid, polyphenol, tannin and antioxidant activity, which all were fitted very well by polynomials of second-degree. Moreover, the significant positive correlations were revealed between polyphenol content and antioxidant activity in all kinds of wines, which implied that polyphenol exerted more influence on antioxidant activity compared with flavonoid and tannin in this study. The result of sensory evaluation exhibited wines with rose had higher scores than those wines only from citrus.
  • CHEN Bin et al
    Food and Fermentation Industries. 2018, 44(8): 166-171. https://doi.org/10.13995/j.cnki.11-1802/ts.016650
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    In this paper, the influence of the Daqu, Xiaoqu and the fermentation way on Fen-flavor Baijiu flavor was studied. Through the traditional Baijiu fermentation method, it was found that the content of ethyl lactate, beta-phenol and acetaldehyde was high in Xiaoqu Baijiu. Liquid-state fermentation can produce a large number of ethyl lactate, ethyl formate, n-propyl alcohol, isobutanol, reactive pentyl alcohol, isoamyl alcohol, beta-benzyl alcohol, etc, but the wheat and corn liquid-state fermentation can produce more acetaldehyde and aldehyde acetal. Overall, the important degree of influence of each factor on Baijiu flavor from high to low was as follows: the fermentation way, Daqu and Xiaoqu, sorghum from the different origin.
  • WANG Gen-jie et al
    Food and Fermentation Industries. 2018, 44(8): 172-176. https://doi.org/10.13995/j.cnki.11-1802/ts.017069
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    To explore the effect of EC content in Cabernet Sauvignon wine fermented by different treatment, three yeasts (ST, BO213, F15) and different dosages of yeast nutrients (0, 0.3, 0.6 g/L) were selected for the wine fermentation. The results showed that EC content in Cabernet Sauvignon wine fermented by different yeasts and different dosages of yeast nutrients were significantly different. The EC content order in the wine fermented by different yeasts was BO213>F15>ST, while the EC content order in the wine fermented by different dosages of yeast nutrients was YN0.6>YN0.3>YNCK. During the wine fermentation, the EC content was less both at the beginning and middle of fermentation, while the EC content accumulated at the end of wine fermentation. The results showed that the EC production in Cabernet Sauvignon wine ranged from 1.9 to 8.0 μg/L and the average concentration of 4.0 μg/L did not exceed the EC limit in wines set by the United States or the European Union.
  • MEI Jian-feng et al
    Food and Fermentation Industries. 2018, 44(8): 177-180. https://doi.org/10.13995/j.cnki.11-1802/ts.016153
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Serrapeptase is a kind of alkaline protease from Serratia marcescens. Due to its excellent anti-inflammatory and anti-swelling function, serrapeptase has been applied to treat some diseases in clinical for many years. In order to obtain strains with the characteristics of fine hereditary stability and prodigiosin negative for high-yield production of serrapeptase, a strain isolated from the intestine of silkworm (Bombyx mori), S. marcescens LL-413 was mutated by ultraviolet and microwave continuously. As results, 2 strains, designated as MW-9 and MW-14, were selected out. Their productivity of serrapeptase was 982 U/mL and 956 U/mL, which respectively was 1.98 and 1.92 times higher than that of the original strain.
  • LIU Li-li et al
    Food and Fermentation Industries. 2018, 44(8): 181-187. https://doi.org/10.13995/j.cnki.11-1802/ts.016057
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    In order to improve the processing characteristics of egg albumin, effects of the synergistic modification of enzymatic hydrolysis and glycosylation on the structure properties of ovalbumin were studied. Glycosylation process of ovalbumin hydrolysates was optimized by using response surface methodology. The optimal process conditions were obtained as follows: pH 9; protein concentration 8 g/100mL; OVA peptides/saccharide ratio 1∶1.5; reaction time 4.5 h; reaction temperature 65 ℃. Under the above conditions, the maximum degree of grafting of (35.67±0.74)% was obtained. The results showed that the emulsifying activity index and the emulsion stability increased by 51.20 m2/g and 38.14%, respectively. The foaming ability and the foam stability increased by 121% and 31.60%, respectively. The ovalbumin before and after the synergistic modification were characterized by SDS-PAGE, FT-IR, fluorescence spectroscopy. Results showed that, after synergistic modification, the molecular weight of ovalbumin was changed from 44.3 kDa to 45.0~97.2 kDa, FTIR result showed that secondary structure was damaged, Dextran was connected to the VOA, some wave length increased. Scanning electron microscopy (SEM) analysis showed that the microstructure of OVA modified by synergistic modification changed from spherical to lamellar structure, and was more compact.
  • DING Ping et al
    Food and Fermentation Industries. 2018, 44(8): 188-195. https://doi.org/10.13995/j.cnki.11-1802/ts.016104
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Methoxylation of commercial citrus pectin in anhydrous methanol catalyzed by HCl was conducted. Reaction conditions including reaction time, temperature, solid-to-liquid ratio (SLR) and HCl concentration were investigated and optimized. High methoxyl pectins with various degree of methoxylation (DM) of >90% were obtained. Results of molecular weight distribution showed that DM was improved with the increase of reaction temperature, reaction time and acid concentration, but the number-average molecular weight was decreased. Particularly, HMP with DM of (91.20±0.13)% was obtained under SLR 1∶50 (w/v) at 60 ℃ with 0.1 mol/L HCl for 12 h, and the Mw was decreased to 15.00 kDa. Furthermore, extremely high DM emulsification properties were studied due to its amphiphilic property (by hydrophobic methoxyl groups and hydrophilic hydroxyl groups) and short molecular length. Nano-emulsions with φ (oil)= 10%-30% were prepared by high energy method (high speed shearing) and their 7-day stability, Zeta-potential and particle size were characterized. Emulsion of 3 500 nm with good stability was produced by high methoxyl pectin with DM of 91.20%.
  • ZHOU Xin-li et al
    Food and Fermentation Industries. 2018, 44(8): 196-202. https://doi.org/10.13995/j.cnki.11-1802/ts.014987
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Freeze-dried fruits and vegetables can greatly preserve the original nutritional value and sensory quality of the food. However, freeze-dried food are not popular in food consumption field due to low drying rate, long drying time and high energy consumption. In this study, carrots were processed by three kinds of method: UHA pretreatment combined with ultrasonic assisted freeze drying, blanching pretreatment with hot air drying and blanching pretreatment combined with freeze drying. The qualities of carrots after three processes were compared. After UHA pretreatment and ultrasonic assisted freeze drying, the volume of carrots sample was decreased by 17.78%; puncture force in external and core decreased, and the change of color were relatively small; carotene content was (69.51±0.20) mg/100g, Vc content was (48.02±0.62) mg/100g and rehydration rate was 6.93, which were the highest in all treatments. The ultrasonic group had better performance in terms of product's texture, carotene content, Vc content and rehydration rate, which showed the superiority of ultrasonic technology in the freeze drying of fruits and vegetables.
  • WEI Yong-hua et al
    Food and Fermentation Industries. 2018, 44(8): 203-210. https://doi.org/10.13995/j.cnki.11-1802/ts.015822
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    The effects of applied pressure, holding time, protein concentration, CaCl2 and pH on properties of whey protein isolated (WPI) gel processed by ultra-high pressure (UHP) were investigated. Only treated by UHP on the condition of applied pressure≥300 MPa, holding time ≥10 min and protein concentration ≥120 g/L, would WPI pressure-induced gel be formed. And the disulfide bond was increased with time, pressure and WPI concentration. These conditions provide gels with finer structure, higher strength and yield and better water-holding capacity. Above the isoelectric point, especial for neutral environment, pressure-induced WPI gels had better gel properties, higher disulfide bond and finer structure. The CaCl2 had no changes on disulfide bond content, but can increase the gel strength by the bridge bond. Yield had negative correlation with CaCl2 concentrations. When the concentration of CaCl2 was 0.06 mol/L, water-holding capacity of WPI gel had a maximum value, but it will decrease with higher concentration of CaCl2.
  • HU Dai-fang et al
    Food and Fermentation Industries. 2018, 44(8): 211-217. https://doi.org/10.13995/j.cnki.11-1802/ts.014916
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    The lye macerating process of beef tripe was optimized by the response surface methodology. Based on the single factor test, response surface methodology was used to optimize the lye macerating processing parameters using concentration of NaOH, lye macerating time and temperature as variables. The producing rate and the water shrinkage rate were selected as evaluating indexse. A quadratic polynomial mathematical model was built between the parameters and the evaluating indexes. Response surface results show that the effect of concentration of NaOH was the most significant, and the optimal lye macerating process was obtained at concentration of NaOH of 5.2 g/L, lye macerating time of 33 min, and lye macerating temperature of 47 ℃. The sensory evaluation is 8.3, the producing rate was 261.5% and the water shrinkage rate was 12.1%,which was consistent with the predicted value.
  • PENG Lin et al
    Food and Fermentation Industries. 2018, 44(8): 218-223. https://doi.org/10.13995/j.cnki.11-1802/ts.016059
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Fresh "jiu ye qing" peppercorn was sterilized by steam and dried by microwave drying. The microwave drying process was optimized by using the response surface method. The influence of microwave power, volume volume and microwave time on the content of hemp flavor was studied. A single factor test was used to select the best condition range. Based on the single factor test, according to the Box-behnken Center combination test design principle, the optimum test design is carried out, and the microwave drying regression model is established by using design-expert.v8.0.6 software, and the maximum value is set by using the content of hemp flavor substance as the index. The results show that the optimum condition of microwave drying index for green pepper is: microwave power 357.78 W, 203.38 g, microwave time 49.14 s. Under the above conditions, the content of hemp flavor is 24.328 7 mg/g. The test results are verified and the error of the optimization results is 1.18%, which indicates that the optimization is reliable.
  • YAN Min et al
    Food and Fermentation Industries. 2018, 44(8): 224-230. https://doi.org/10.13995/j.cnki.11-1802/ts.016031
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    In this paper, red lemons were used as the main material, the clarification effects of pectinase, complex enzyme, chitosan, bentonite and diatomite on the red lemon juice were studied with light transmittance as the index. The best clarifying agent for the fruit juice was selected. According to single-factor experiments and Box-Behnken experimental design, the optimized clarification process of red lemon juice was obtained. The results showed enzyme and chitosan were better in the clarifying process of the juice. In contrast, bentonite and diatomaceous earth had little effects. Considering the economy, convenience and clarification of the quality of red lemon juice, pectinase was selected a clarifying agent for red lemon juice. The optimum conditions of clarified red lemon juice were: 0.22 g/100mL pectinase, at 50.3 ℃ with pH 4.3 for 62 min, the red lemon juice transmittance was as high as 93.8%.
  • DING Jing et al
    Food and Fermentation Industries. 2018, 44(8): 231-238. https://doi.org/10.13995/j.cnki.11-1802/ts.016072
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    The effects of different treatments including clove extract, phystic acid, rapeseed, BHT and VE on the functional properties of myofibrillar protein were studied using fresh loach as raw material. The results showed that with the prolongation of storage time, the functions in all loach MP were gradually weakened, but compared with the control group, the carbonyl content of antioxidants and surface hydrophobicity were increased in the treatment group, and the decreased of sulphur content, solubility, EAI and ESI, thermal induced gel hardness, elasticity, water retention, and browning were all controlled to a certain degree. The addition of antioxidants can significantly inhibit the oxidation of myofibrillar protein, and better maintain the functional properties of myofibrillar protein. The antioxidant ability of clove extract, phytic acid and synthetic antioxidant BHT was better than rapeseed and VE.
  • GONG Shu-bei et al
    Food and Fermentation Industries. 2018, 44(8): 239-245. https://doi.org/10.13995/j.cnki.11-1802/ts.017270
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    In order to explore differences of volatile and non-volatile compounds between traditional and mechanical raw Baijiu of roasted-sesame-like aroma type Baijiu(Chinese liquor), the liquid-liquid microextraction (LLME) and hexamethyldisilazane (HMDS) derivatization coupled with gas chromatography-mass spectrometry (GC-MS) and other methods were employed. 94 kinds of compounds were detected both in traditional raw Baijiu and mechanical raw Baijiu. The results showed that the total concentrations of compounds in traditional raw Baijiu were higher than mechanical raw Baijiu. Except acids, phenols and lactones, the total concentrations of the other compounds in traditional raw Baijiu were higher than those in mechanical raw Baijiu. By comparing the concentrations of the components in the two crafts of Baijius, 12 compounds were significantly different, including 4-ethylphenol, 4-ethylguaiacol, 1,1,3-triethoxypropane, 2-hydroxybutyric acid, butyl lactate, 3-hydroxybutyric acid, 1-butanol, 2-furanmethanol, furfural, isoamyl butyrate, 2-phenylethyl butyrate, and 3-hydroxypropionic acid.
  • WANG Cheng-cheng et al
    Food and Fermentation Industries. 2018, 44(8): 246-251. https://doi.org/10.13995/j.cnki.11-1802/ts.016909
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    A methodology for rapid determination of key "caramel aroma" compound (sotolon) in Chinese rice wine by liquid-liquid microextraction (LLME) combined with gas chromatography-mass spectrometry (GC-MS) was developed. The detective and quantitative limits of the method were 0.66 μg/L and 1.02 μg/L respectively, the recoveries varied from 80% and 120%, the intraday and inter-day precision were within 10%, which suggested that the method could meet the needs for rapid testing. The methodology was successfully applied to determine the content of sotolon in 24 typical rice wine samples from different regions, and the results showed that sotolon was a common aroma compound in rice wine with the concentration ranging from 42.05 to 244.34 μg/L. The odor threshold of sotolon (11 μg/L) in rice wine was determined for the first time. The aroma contribution of sotolon in rice wine was studied based on the odor activity value (OAV), and the results showed that the contents of sotolon in all the samples were higher than the odor threshold, and thus indicated that sotolon played an important role in the overall aroma of rice wine. In addition, the contents of sotolon in rice wine from Zhejiang and Shanghai regions were significantly higher than those from other areas, which further indicated that it is a potential compound for distinguishing rice wines from different regions.
  • ZONG Jie et al
    Food and Fermentation Industries. 2018, 44(8): 252-257. https://doi.org/10.13995/j.cnki.11-1802/ts.016579
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    In this study, the rapid detection method based on quartz crystal microbalance gas sensor (QCM sensor) using amine-functionalized mesoporous silica nanoparticles as a sensing material was constructed for determination of formaldehyde in beers. The morphology structure and physicochemical property of materials were characterized by scanning electron microscopy, transmission electron microscopy and nitrogen adsorption-desorption. The formaldehyde sensing performance of NH2-MSN-based sensor and the detection of formaldehyde in beers were systematically investigated. The results showed that mesoporous silica nanoparticles had an average diameter of 100 nm and pore diameter of 3.5 nm, and the NH2-MSN-based sensors exhibited a low detection limit of 0.5 mg/L, a rapid response (7 s) and recovery (12 s), as well as excellent sensitivity, selectivity and stability. Furthermore, the as-fabricated sensors were utilized for determination of formaldehyde in beers. Each sample could be detected within 30 s and showed a good linear relationship. This method was fast, sensitive, and high efficiency, which had wide application prospect for the detection of formaldehyde in beers.
  • ZHANG Pei-min, et al
    Food and Fermentation Industries. 2018, 44(8): 258-264. https://doi.org/10.13995/j.cnki.11-1802/ts.016434
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Because of the restriction of stable carbon isotope ratio techniques, the database construction has become the premise of the application of stable isotope technique on evaluation of wine. Thus, stable isotope internal standardization has been a research focus of establishing isotope fingerprint in food field. In this study, the δ13CVPDB-values of tartaric acid and sugar in musts and the δ13CVPDB-values of tartaric acid and alcohol of corresponding wine have been measured. The δ13CVPDB values of tartaric acid in musts from four regions ranged from -24.48‰ to -20.02‰, the δ13CVPDB values of sugar in musts ranged from -28.13‰ to -23.25‰, the δ13CVPDB values of alcohol in alcohol fermentation ranged from -29.93‰ to -24.23‰. Meanwhile, good correlation (R2=0.94) were obtained between tartaric acid and sugar in musts. Also in alcohol fermentation, good correlation (R2=0.96) were obtained between tartaric acid and alcohol. Adding different proportions of C4 sugar to musts and alcohol to wines, the δ13CVPDB of sugar and alcohol displayed positive distribution, but δ13CVPDB of tartaric acid remained unchanged. The δ13CVPDB values of tartaric acid remained unchanged in alcohol fermentation(|Δ13CVPDB|<0.1‰). Based on this point, tartaric acid as internal standardization could be applied to identify the authenticity of must and "no-additive" wine. The recognition rate obtained by ratio model was higher than that by different model.
  • YANG Cheng-cong, et al
    Food and Fermentation Industries. 2018, 44(8): 265-270. https://doi.org/10.13995/j.cnki.11-1802/ts.017059
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    In the present study, the effect of socking time of glutinous rice on the flavor quality of Chinese rice wine was explored by GC-MS and electronic nose using volatile components as the indicators. The results showed that alcohol and ester compounds were the main volatile flavor in Chinese rice wine. Cluster analysis showed the flavor quality of Chinese rice wine might change significantly when the socking time was more than 11 d. Mann-Whiney test also indicated that the aromatic substance of volatile components of Chinese rice wine decreased significantly when the socking time was more than 11 d (p<0.05). Thus, we concluded that the soaking time of less than 11 d was benefit for the fermentation of Chinese rice wine.
  • CHENG Xin et al
    Food and Fermentation Industries. 2018, 44(8): 271-276. https://doi.org/10.13995/j.cnki.11-1802/ts.015240
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Fruiting body components of Agrocybe cylindracea between the first three tides were analyzed and compared by the national standard(GB) test method according to FAO and WHO evaluation criteria. The results showed that crude protein contents in the fruiting body of first tide were higher than those in the second and third tides, but the crude fat content were not significant difference among three tides. Amino acid composition of the fruiting body of three tides were identical in terms of category but different in the content. The contents of total amino acids of the third tide were significantly higher than that in the other two tides. Heavy metals (Pb, Cd, Hg and As) contents in fruiting body of the third tide were significantly higher than the other tides, but the contents in all of the three tides did not exceed national food safety standards, and met the requirements of green food (edible mushrooms). By evaluation of these indicators, we know that quality of the third tide of Agrocybe cylindracea was better on protein and amino acids concentrations, but the heavy metals contents was also higher.
  • ZHANG Jie, et al
    Food and Fermentation Industries. 2018, 44(8): 277-283. https://doi.org/10.13995/j.cnki.11-1802/ts.016113
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    The physical properties and chemical composition of Sichuan white goose at different age and different body part were determined. The results showed that age was the main factor influencing the quality of Sichuan white goose among three factors (age, location and sex). With age increased (70-days-old to 2-years-old), the meat pH decreased first and then increased, the lowest was found in 120-days-old goose; L* and a* showed an increasing trend, which significantly increased at 1-year-old (p<0.05), and b* showed a steady decreasing trend. The cooking loss rate showed a trend of increasing first and then decreasing, the highest was in 90-days-old goose. The shear force value increased, and the chest muscle was significantly increased in 150-days-old googse (p<0.05). The leg muscle increased very significantly at 1-year-old goose (p<0.01). The water content showed a tendency to decreased first and then increased, and 90-days-old goose showed the highest, however, it decreased sharply at 120-days-old goose (p<0.01), and increased significantly at 1-year-old goose(p<0.01). The overall change in ash content is very small between 1.1% and 1.5%. The content of crude protein increased first and then decreased, and the chest and leg muscle reached the highest at 150-and 120-days-old goose, respectively. Intramuscular fat content was increase and then increased: the chest and leg muscle reached the highest at 120-and 90-days-old goose, respectively. These results showed that it was difficult to judge the quality of Sichuan white goose from different ages. Overall, the quality of Sichuan white goose was good at from 90-days-old to 150-days-old. Therefore, Sichuan white geese were better slaughtered in the above age range.
  • LI Huan-huan et al
    Food and Fermentation Industries. 2018, 44(8): 284-290. https://doi.org/10.13995/j.cnki.11-1802/ts.016116
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    A 10-member sensory panel was formed after training to evaluate Fuzhuan brick-tea. In order to optimize the sensory evaluation of Fuzhuan brick-tea, a market brick-tea was selected and 18 samples with different tea-water ratio, brewing time and inlet temperature were made according to the response surface method. After collecting the characteristics of aroma and flavor of 18 Fuzhuan brick-teas, 11 sensory attributes were confirmed. Based on the data of quantitative description analysis, principal component analysis and variance analysis showed that the overall flavor, acid, sweet, bitter, astringent, overall aroma and bitter butyl were important attributes to distinguish the sensory differences between samples. The result of response surface analysis showed that the stability of the sensory evaluation was better under low ratio of tea-water and short brewing time. Considering the intensity response values of flavor and aroma, the optimal sensory evaluation conditions for Fuzhuan brick-tea was: tea-water ratio 1∶100, brewing time 5.00 min and drinking temperature 50 ℃.
  • WANG Hui, et al
    Food and Fermentation Industries. 2018, 44(8): 291-297. https://doi.org/10.13995/j.cnki.11-1802/ts.016960
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Yarrowia lipolytica is known as a kind of unconventional yeast, it has been widely used in various industries due to its unique physical and chemical properties, and yeast metabolic characteristics. This yeast is widely used in food industry because it belongs to GRAS microorganisms. The application of Yarrowia lipolytica in the food industry was reviewed in this article. Yarrowia lipolytica mainly can be used in production of dairy and meat products, aromatic compounds, polyols and organic acid, and surfactants and emulsifiers. Furthermore, it can cause food corruption, production of be used as a single cell lipid and single cell protein, and be involved in the degradation of food waste and transformation. This paper clarified the importance and broad application prospect of Yarrowia lipolytica in food industry.
  • HAO Jun-guang, et al
    Food and Fermentation Industries. 2018, 44(8): 298-307. https://doi.org/10.13995/j.cnki.11-1802/ts.016631
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    It is common limiting technological issue and research focus of whole world brewing industry to improve flavor stability, including bitterness stability, with an aim to keep refreshing character of beer as long as possible to attract consumers in the shelf life of beer. Beer bitterness is mainly endowed by soft resin (i.e. α/β-acids), hard resin as well as their derivatives. With the help of modern purification technologies, nuclear magnetic resonance spectroscopy and high resolution HPLC MS, the overseas researchers have took some breakthroughs in identifying the compounds of α/β-acids derivatives and complicated hard resin. In this paper, the highlights of the newly identified bitter compounds originated from hop β-acid and hard resin were reviewed in detail, especially the relevant proposed reaction mechanisms, thresholds and elution of them during brewing and storage were introduced, in order to let beer brewing peers in China to enlarge knowledge of the complicated beer bitter quality in depth.
  • ZHANG Hong-ru, et al
    Food and Fermentation Industries. 2018, 44(8): 308-313. https://doi.org/10.13995/j.cnki.11-1802/ts.016330
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Although new soybean yogurt is gradually popular as a new nutrition health food for people, it is lack in China. There are some deficiencies in the flavor and fermentation technology. Based on domestic and international latest researches, the progresses on removal of beany flavor, fermentation strains and other aspects were reviewed in this paper. Some physiological functions, such as enhancing immunity, anti-cancer, lowering cholesterol by soybean yogurt was introduced in this paper. The prospect of the new soybean yogurt was expected in this paper.
  • LIU Zhen-ya, et al
    Food and Fermentation Industries. 2018, 44(8): 314-321. https://doi.org/10.13995/j.cnki.11-1802/ts.015993
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Potato tuber is known as a nutritious food because of its rich in protein, dietary fiber, vitamins and minerals the human body needs. Potato flour is a kind of granular or powder product made by potato tuber through multiple processes and retains the nutrients and flavor of potatoes to the greatest extent and is easier to store and transportation. Therefore, it has become an important product in potato deep processing industry. In this paper, the classification, characteristics and processing technologies of potato flour are briefly introduced and the key technologies such as the selection of raw materials, peeling, color protection, cooking, drying and reduction of cell damage rate are discussed. The recommendations on promoting production of high-quality potato flour are put forward aiming to boost the healthy and sustainable development of potato flour processing industry and accelerate the strategy of using potato as the main food source.
  • WANG Xuan, et al
    Food and Fermentation Industries. 2018, 44(8): 322-327. https://doi.org/10.13995/j.cnki.11-1802/ts.015186
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    卵磷脂广泛存在于自然界,是重要的功能性成分之一,对其进行高效分析具有重大意义。食品中卵磷脂含量的检测方法主要有紫外可见分光光度法、薄层色谱法、高效液相色谱法、核磁共振波谱法、红外光谱法和质谱分析法等6种方法,分别对以上6种方法进行概述,并对各自方法的优缺点进行了初步探讨,旨在为从事食品中卵磷脂检测技术研究提供参考。