25 February 2011, Volume 37 Issue 02
    

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    Food and Fermentation Industries
  • Dong Xiu-ping, Zhu Bei-wei, Qi Li-po, Zheng Jie, Zhang Shuang, Jiang Dan
    Food and Fermentation Industries. 2011, 37(02): 1-6. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.029
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    In order to study the changes of collagen from sea cucumber body wall during heating process,the AFM method was adopted to investigate the influence of sample concentration,solvents,heating processing temperature and heating time on the aggregation states of PSC from sea cucumber body wall.The results showed that in acetate buffer(pH 2.7),PBS buffer(pH 7.2) and ultra-pure water solution,collagen self-assembled gradually as the concentration increased and net-work of random coils or schistose structure were formed eventually.But the concentration of PSC needed to start self-assemble was different in the solutions mentioned above.The collagen self-assembled gradually to net-work as the heating temperature rised or the heating time prolonged.But,when the temperature was higher than 100 ℃ or the time was longer than 2 h,its crosslinking degree would decrease and the net-work structure disappeared gradually.
  • Xie Yin-zhu, Shen Wei, Wang Zheng-xiang
    Food and Fermentation Industries. 2011, 37(02): 7-10. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.001
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    According to the sequence of pullulanase gene from Bacillus deramificans(NCBI accession number: AX203843),the gene encoding mature peptide of pulluanase was synthesized and designated pulA.The pulA was amplified by the method of PCR and cloned into the expression vector pHY-WZX,yielding hybrid plasmid pHY-WZX-pulA.Subsequently,pHY-WZX-pulA was introduced into Bacillus licheniformis B60608.Active pullulanase was expressed by recombiant B.licheniformis and secreted into medium.Culture condition of recombinant B.licheniformis were optimized for production of pullulanase.The optimized medium consists of 2 % cotton seed protein and 8 % of glycerol.
  • Yao Jing, Ren Jing, Wu Zheng-jun, Wang Yin-yu, Guo Ben-heng
    Food and Fermentation Industries. 2011, 37(02): 11-16. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.002
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    Exopolysaccharides is produced by Lactic acid bacteria which is General Recognized As Safe status(GRAS).As its excellent functions,it is becoming a research hotspot recently.However,its industrial application is limited by the low production level.Therefore,it is significant for studying the genetics to improve the production.This paper mainly concerns about the classification and structure of exopolysaccharides,biosynthesis of homopolysaccharides and heteropolysaccharides,besides the discrepancies between these two exopolysaccharides.Genetic regulation and some effective regulation measures are closely discussed as well.This paper should provide a theoretical reference for further research.
  • Long Zhen, Ruan Qi-Jun, Kong Xiang-zhen, Zhang Cai-meng, Hua Yu-fei, Jiang Bo
    Food and Fermentation Industries. 2011, 37(02): 17-20. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.030
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    Amaranthus tricolor leaves were identified as a particularly rich source of HPL activity.Hydroperoxide lyase(HPL) was purified to electrophoretic homogeneity from high speed centrifigation,solubilized by Tritron X-100,ammonium sulfate precipitation,hydroxypatite chromatography and DEAE Toyopearl chromatography.The purified HPL preparation consisted of a single band with a molecular mass of about 55 ku in SDSPAGE.The HPL showed higher activity against 13-hydroperoxy-linolenic acid compared to 13-hydroxy-linoleic acid.Maximum HPL activity was observed at pH 6.0 and 25℃,and room temperature sustains high enzyme activity.
  • Chen Yuan-yuan, Shi Gui-yang, Wang Zheng-xiang
    Food and Fermentation Industries. 2011, 37(02): 21-23. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.003
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    D1/D2 domain of 26S rDNA sequence-based alignment and analysis is a widely used method for yeast identification and classification.Rapid and repeatable protocols to extract and prepare the chromosomal DNA of yeast are the basic needs to acquire the templates for following amplification of D1/D2 domain.However,the present methods to extract chromosomal DNA are time-consuming and unable to deal with large-scale samples at the same time.In the present study,a novel rapid and efficient method to identify a large amount of yeast isolates in the same batch was established.The chromosomal DNA required only 20 minutes to prepare and can be directly used as template for PCR amplification.Results of amplified ITS sequences were clear and easy to identify by sequencing analysis.
  • Jin Rong, Gu Zheng-biao, Hong Yan, Cheng Li, Li Zhao-feng
    Food and Fermentation Industries. 2011, 37(02): 24-30. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.031
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    The molecular weight distribution of maltodextrins and its relation to the synthesis of cyclodextrinsCyclodextrins(CDs) are cyclic oligosaccharides with a wide range of application in food,pharmaceutical,cosmetic and agricultural industries.In this work,we studied the influence of molecular weight distribution of starch degradations on the synthesis of β-CD.The molecular weight distribution of starch degradations were analyzed by GPC on both Sephacryl 200-HR and Sephacryl 400-HR column.The oligosaccharides in starch degradation were analyzed by HPLC.With the increasing of DE,the contents of low weight polysaccharides with higher multiple branching degree increased.Maltodextrins,which were prepared by different methods,had significantly different molecular weight distribution even if they had similar dextrose equivalent value(DE).In general,the conversion rate of β-CD decreased with DE increasing.The best condition for the substrate is the average molecular weight between 43 053~18 793 g/mol and less branch Maltodextrains.Similar DE with different processing methods can shift molecular weight distribution greatly.The different component of Maltodextrains can affect β-CD production.Starch degradations with appropriate molecular weight and higher linear degree are better for the synthesis of β-CD.
  • Li Hai-long, Fu Xiao-yan, Chen Peng, Cherif.I K Diop, Xie Bi-jun, Sun Zhi-da
    Food and Fermentation Industries. 2011, 37(02): 31-36. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.032
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    The effect of volume ratio between acetic acid and acetic anhydride on the degree of substitution(DS) was investigated for the synthesis of starch acetates by microwave assistance.Chemical structure、granule morphology、crystalline、thermal properties and molecular weight distribution of starch acetates were all determined separately by infrared spectrum(IR)、scanning electronic microscope(SEM)、X-Ray Diffractomer(XRD)、differential scanning calorimeter(DSC)and gel permeation chromatography(GPC).The physicochemical characteristics for starch acetates synthesized under different acetic acid to acetic anhydride ratio were also compared on the basis of those results.The results were obtained as follows:the ratio between acetic acid and acetic anhydride had a great effect on DS;DS was reached to the highest of 2.92 at the ratio of 1∶ 1(volumn ratio);and then,DS decreased along with the increase of acetic anhydride.Moreover,the chemical structures and physical-chemical properties had a great changes after the esterification.The hydroxyl groups of each glucose unit were replaced by acetyl groups gradually as the increase of DS.As it is shown from SEM results,the surface of starch acetate became rough and porous.Meanwhile some of the starch particles have been cracked into tiny parts;After modification,new crystal formed and old intrinsic crystalline structure destroyed.As a result of that,the degree of crystallization decreased.The thermal properties of starch acetates have also changed greatly with the decline of glass transition temperature(Tg) and melting temperature(Tm).Therefore,starch acetates are expected to exhibit better thermoplasticity than native starch.The molecular weight of starch acetates were decreased and its distribution became wider,this indicated that starch chain was broken during esterification.
  • Liu Peng-fei, Dong Hai-zhou, Hou Han-xue, Liu Chuan-fu, Ren Hong-tin
    Food and Fermentation Industries. 2011, 37(02): 37-40. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.033
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    The property improvement of starch-based edible film by pullulan was studied.The surface of the film was observed with the optical microscope.The result showed that the tensile strength of the starch film was improved effectively by adding pullulan;water dissolution time was shortened and oxygen permeability of the starch film was reduced.When pollulan was 5%~9% in the solution,water permeability was also decreased;the whiteness value of the film was increased and the yellowness value was lower.The optical microscopy analysis of the film surface suggested that the pullulan and the starch were fused better at 9% of pullulan.The conclusion was: the best properties of the starch film was when pullulan's concentration less than 9%.
  • Wen Yan, Zhao Xin-huai
    Food and Fermentation Industries. 2011, 37(02): 41-45. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.034
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    Bovine milk was treated with horseradish peroxidase or/and ferulic acid,and used to prepare set-yoghurt.The textural and rheological properties of the yoghurt samples prepared were measured with Texture Profile Analyzer and Rheometer and compared to that of control.The results showed that treatment of bovine milk with horseradish peroxidase or/and ferulic acid did not impact the main compositions of the yoghurt samples,but clear influence on the texture and rheology.Compared to that of control,the hardness or the adhesiveness of the yoghurt samples with gelatin addition,horseradish peroxidase treatment,or horseradish peroxidase plus ferulic acid treatment had an increase of 38.03,34.07 and 17.08%,or 45.13,40.85 and 28.36%,respectively,while the syneresis of these samples had a decrease of 12.02,6.28 and 3.41%,respectively.Also,the apparent viscosities,thixotropy and viscoelastic behaviors of these samples were in the order of the yoghurt with gelatin addition,the yoghurt with horseradish peroxidase plus ferulic acid treatment,the yoghurt samples with horseradish peroxidase treatment,control.Our results stated that treatment of bovine milk with horseradish peroxidase or/and ferulic acid had a beneficial effect on the quality of set-yoghurt prepared.
  • Hu Jing-rong
    Food and Fermentation Industries. 2011, 37(02): 46-49. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.019
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    The original strain Pythium was mutated by UV combined with LiCl.After preliminary screening by low-temperature culturing and microscope observation after Sudan Ⅱ staining,and re-screening by shake flask fermentation,a high-yielding eicosapentaenoic acid-producing strain Pythium H6 was obtained.Its biomass,oil and EPA content was 23.5 g /L,17.54 % and 15.36 % respectively after two stages of 15~20℃ culture for 168h.In addition,the EPA production of H6 was 633.17 mg/L,which was 140.01 % higher than the original strain,and the product character was stable.The study of the lipid synthesis of Pythium H6 exposed to different concentrations of exogenous citric acid indicated that adding 1.5 g/L citric acid to fermentation medium can significant increase its glucose utilization,lipid and EPA content with EPA yield up to 890.49 mg/L,40.64 % higher compared to the control.Fermentation experiments suggested that Pythium H6 has the potential to be developed into a highly industrial production strain.
  • Wu Wei-ling, Yang Heng, Zhu Wen-liang, Zhou Yong, Xiong Juan, Zheng Jian-xian
    Food and Fermentation Industries. 2011, 37(02): 50-56,61. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.020
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    The liquid-liquid extraction process of modified lipid prepared by lipase catalyzed transesterification was investigated.The effects of extraction solution concentration,material ratio,extraction time on free fatty acid extraction ratio and modified lipid yield were investigated by single factor experiment and response surface methodology.The results show that free fatty acid extraction ratio is increasing with extraction solution concentration,material ratio,extraction time increasing,while yield is decreasing with these three process conditions increasing.The regression model from response surface methodology can highly reflect the relationship of different factor levels and free fatty acid extraction ratio,yield.The sequence of two factors influenced on free fatty acid extraction ratio of modified lipid is extraction solution concentration>material ratio,while the sequence of two factors influenced on yield of modified lipid is reversed,material ratio>extraction solution concentration.The optimal process is obtained by overlaying two contour lines of free fatty acid extraction ratio and modified lipid yield,and the optimal process is enthanol aqueous solution concentration 85.00%,material ratio 1∶ 1.
  • Lin Ri-hui, Xu Li-li, Nong Mian, Zhang Lu-cheng
    Food and Fermentation Industries. 2011, 37(02): 57-61. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.021
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    Oxalate decarboxylase was expressed and characterized in this paper.After induced by IPTG,820 U/g(wet weight) of oxalate decarboxylase was obtained,and the enzyme was purified 2.07 fold by metal chelate affinity chromatography with a yield of 60.38%.The enzyme had an optimum temperature between 50 and 55 ℃,and optimum pH at 3.5.Addition of EDTA or Fe2+ would activate oxalate decarboxylase,but addition of Mn2+ would inhibit the enzyme.Under condition of pH4.0,37 ℃,the enzyme's Km was 14.53 mmol/l with Vmax=133.33 U/mg.
  • Yao Xing-cun, Jiang Dong-lei, Pan Sai-kun, Shu Liu-quan
    Food and Fermentation Industries. 2011, 37(02): 62-64,69. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.022
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    In the paper,the preparation of bioactive oligopeptides from Porphyra yezoensis proteins in the presence of papain was studied.The ACE inhibitory activity was used as response to optimize the enzymatic conditions.The molecular weight distribution of the hydrolysates was determined by gel filtration chromatography on SephadexG-15 column.The result of which showed that the optimum conditions were pH 7.5,temperature 50℃,substrate concentration 30mg/mL,addition amount of enzyme 600 U/g.Under such conditions,and hydrolyzing for 4 hours,the inhibitory activity of the hydrolysates reached up to 30%,with IC50 value reaching 4.48mg/mL.The hydrolysates of Porphyra yezoensis were then determined by chromatography,the result of which showed that the hydrolysates of antihypertensive activity from Porphyra yezoensis proteins was mainly oligopeptide with molecular weight less than 2000.
  • Zhao Xi-heng, Li Jin-cai
    Food and Fermentation Industries. 2011, 37(02): 65-69. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.006
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    In this work,relationship between the senescence of broccoli(Brassica oleracea L.Var.italica) florets and their changes in antioxidative level was investigated.Postharvest broccoli florets were stored at 20℃,and its indicators of senescence(chlorophyll,protein and malondialdehyde(MDA) contents) and antioxidative level(ascorbic acid and β-carotene contents,superoxide dismutase(SOD),catalase(CAT) peroxidase(POD) and ascorbate peroxidase(APX) activities) of flower buds were analyzed in continuous 4 days during the storage.With increasing of the storage time,chlorophyll and protein contents decreased,MDA content increased,ascorbic acid and β-carotene contents decreased,SOD,CAT and POD activities increased and APX activity decreased.These antioxidative level indicators showed linear correlation to senescence indicators at P=0.01.These results showed that senescence of postharvest broccoli florets is closely correlated to antioxidative level.
  • Li Zhi-ying, Cheng Li-li
    Food and Fermentation Industries. 2011, 37(02): 70-72. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.007
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    Microwave method was used to extract astragalus polysaccharides fromβ-cyclodextrin and its derivatives.Under simulated human gastric juice,the removing capability of astragalus polysaccharides on the nitrite was determined by spectrophotometry.The experimental results indicated that β-CD can improve the capability of scavenging sodium nitrite from 29.1% to 50.2%,and the optimum conditions for the extraction were as follows: microwave power 455w,extraction time 2 min and the removing capability of astragalus polysaccharides to the nitrite was 51.4%.
  • Liu Su-wen, Shi Peng-bao, Li Han-chen, Li Jun, Gao Hai-sheng
    Food and Fermentation Industries. 2011, 37(02): 73-77. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.035
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    The conditions for decolorization of apple dietary fiber were established for laboratory study.The microwave method was applied to the optimal conditions for decolorization of apple dietary fiber.In this study,the optimal conditions determined by response surface methodology(RSM) were: pH 12.5、microwave time 47.75s and H2O2 concentration 9.17%,material-solution ratio 1∶ 14 and microwave power 480W.Under the above conditions,the Whiteness was 57.34%(mean value) in the final product,while 27.54% before the decolorization.The effect was significant.The empirical model developed by response surface methodology(RSM) is adequate to describe the relationships between the factors and response value.The results of the curve by scanning electron and X-ray diffraction analysis showed that microstructure of the apple dietary fiber surface was changed by microwave.
  • Liu Huan, He Lian-bin, Wei Jing, Ma Zhong-su
    Food and Fermentation Industries. 2011, 37(02): 78-81. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.036
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    This paper studied the influence of cellulase and hemicellulase on the length,width,sugar content and polymerization degree of carrot pomace fiber.The mechanism of synergy of cellulase and hemicellulase modified carrot fiber was analyzed.The result indicated that the complex application of cellulase and hemicellulase was more obvious effect than the single enzyme.And the synergism of cellulase and hemicellulase could improve the thin fibrosis of the fiber surface,enhance the production rate of enzymolysis fiber,and reduce the polymerization degree of the fiber.
  • Zeng Xiao-man, Liu Bei-yu, Zhong Geng, Peng Jia-ying, Xia Yu-hong
    Food and Fermentation Industries. 2011, 37(02): 82-86. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.013
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    In order to improve the physic-chemical properties of Konjac glucomannan and extend its application field,konjac glucomannan was modified with hydrophilic carboxymethyl group and hydrophobic stearate group by microwave.The effects of the amounts of the methyl stearate,sodium hydroxide,microwave reaction time,and the temperature on the degree of substitution(DS) were studied by single factor experiments.Based on these studies,the optimum reaction parameters of preparing SCMK were obtained by revolving orthogonal combinatorial design experiment,and its physical-chemical properties were investigated.The results showed that the amount of the methyl stearate,the amount of the sodium hydroxide,reaction temperature and time were about 14.0%~14.4%,5.85%~6.36%,85.9~87.5℃ and 10.1~10.4min,respectively.The percentage of SCMK with DS value of 0.020 was more than 95%.Compared with the carboxymethyl konjac glucomannan,the viscosity of the SCMK increased,the ability of anti-alkaline and emulsifying capacity and its stability improved.SCMK is a better emulsifier.
  • Liu Xiao-fei, Cheng Chun-sheng, Qin Yu-yue, Shun Sha, Zhang Zhi-hong
    Food and Fermentation Industries. 2011, 37(02): 87-90. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.008
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    The objective of this paper was to investigate the effect of plasticizer on properties of chitosan /nano-montmorillonite film.The results showed that plasticizer had significant effect on the properties of the film.It reduced the tensile strength and light transmittance,significantly increased the elongation at the break,and increased the water vapor permeability and water absorption.
  • Jin Hong-xing, Shi Jian-bo, Cheng Wen-yu, Ju Xia-yan
    Food and Fermentation Industries. 2011, 37(02): 91-93,98. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.023
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    The production of mannitol by Leuconostoc mesenteroides CGMCC 1.10327 using sucrose(2%,m/v) as the carbon source was investigated in batch culture fermentation.To optimize the production of mannitol,the effects of glucose(5 g/L),three salt nutrients(K2HPO4,NaAC,and ammonium citrate),various initial culture pHs,and 0.2% CaCO3 on the production of mannitol were investigated,respectively.The results showed that glucose contributed to improve the yield of mannitol.Three salt nutrients(K2HPO4,NaAC,and ammonium citrate) had significant effects on the production of mannitol,and the highest yield of mannitol was obtained when three salt nutrients were 2 g/L,5.0 g/L,and 2 g/L,respectively.The highest yield of mannitol was obtained at initial pH 6.0.Moreover,the yield of mannitol was significantly decreased by adding CaCO3.
  • Zhou Yu-heng, Chen Hai-shan, Cai Ai-hua, Tan Xiang-xiang, Zhang Hou-rui
    Food and Fermentation Industries. 2011, 37(02): 94-98. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.024
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    In this paper,steam explosion in various conditions was studied in order to reveal its impact on preparation of xylooligosaccharides from sugarcane leaves by enzymatic hydrolysis.The results demonstrated that steam explosion could effectively break down the surface of materials and make it easier for enzymetic hydrolysis.The addition of acid or alkli facilitated xylan's release.On one hand,acidic steam-explosion contributed to sugar reduction and was responsible for the poor proportion of xylooligosaccharides in the end-product.On the other hand,alkalic steam-explosion made xylan extraction easy in the form of large molecule and generating less reducing sugar.Higher rate of enzymatic hydrolysis was also attributed to alkalic steam-explosion.Finally,when the steam explosive substance was hydrolyzed by xylanase,it produced 78%~85% xylooligosaccharides including 40%~50% xylobinose.Steam-explosion assisted by alkali was a good method for manufacturing of xylooligosaccharides.
  • Qi Xiang-jun, Xu Ting-ting, Zhang Wen, Ran Deng-yuan
    Food and Fermentation Industries. 2011, 37(02): 99-102. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.004
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    In order to select a high-efficient oil-degrading bacteria,here an oil-degrading bacteria was selected from the lampblack exit of the restaurant,which was named QZX-A.Then,it was identified using the methods such as morphological,physiological and biochemical identification and 16S rRNA.At the same time,its growth performance was anaylzed by the turbidimetric and weighing methods.Furthmore,its ability to produce rhamnolipid was discussed by the method oil-spreading and anthrone-sulfuric acid.Results: On the basis of morphological,physiological and biochemical evidence,strain QZX-A had the characteristics of Pseudomonas aeruginosa bacteria.Phylogenetic analysis based on 16S rRNA gene sequences revealed that QZX-A belonged to a phyletic sub-lineage within the family Pseudomonas,with ≥98% 16S rRNA gene sequence similarity to the described species.In addition,strain QZX-A had a similar growth curve in beef extract peptone and fermentation medium,but the growth rate of the former was bigger than the latter.The result of TLC showed that the metabolic products of strain QZX-A belonged to glycolipid compounds.The oil-spreading round was up to 11cm,and its productivity was 567.75 mg /L.In sum,since strain QZX-A was genetically stable,it had some value of development in the degradation of oil and in the production of rhamnolipid.
  • Liu Nan-nan, Chen Xue-feng, Liu Jun-jie
    Food and Fermentation Industries. 2011, 37(02): 103-107. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.025
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    The manufacture of microencapsu1ation of onion oil flavor by complex coacervation using gelatin and gum arabic as the wall materials was investigated.The influence of the ratio of core to wall,pH and hardening time on the morphology of the microencapsu1ation was evaluated.Core to wall ratio 1∶ 2;pH4.15;the transglutaminase used as cross-linking agent to gelation ratio 25%;solidified time12h were found to be the optimum conditions.Finally,the microencapsulation powders were prepared by spray-drying(inlet temperature was 185℃,outlet temperature was 80℃ and feed temperature was 50℃).The moisture content and encapsulation efficiency of microencapsulation powders was 2.87% and 89.55% respectively.
  • Shi Qi-long, Zhao Ya, Yang Xiao-li, Wang Xi-hai, Zheng Ya-qin
    Food and Fermentation Industries. 2011, 37(02): 108-112. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.037
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    The ratio of raw material to water,the temperature and the proportion of enzyme in coix seed hydrolysis were determined according to the values of DE.The ratio of raw material to water,the temperature and the time duration in buckwheat extracts were determined according to the soluble solid contents.With coix seed juice,buckwheat juice,high fructose syrup,citric acid,and water as experimental factors,with sensory quality evaluation as index,the best formula of the mixed juice was determined by mixture design.With guar gum,xanthan gum,sodium alginate and monoglyceride as experimental factors,with viscosity,centrifugal separation percentage,and standing layered percentage as indexes,the optimum compound stabilizers were also determined through orthogonal experiment.The results showed that the optimal parameters of hydrolysis of coix seed were: the ratio of raw material to water 1∶ 10,temperature 500°C and the proportion of enzyme of 0.06g/10g.The optimal parameters of extraction of buckwheat were: the ratio of raw material to water 1∶ 10,800°C for 60min.The best formula of the compound juice was coix seed juice 30%,buckwheat juice 5%,high fructose syrup 5%,and water 60%.Guar gum,xanthan gum and sodium alginate were compound stabilizer with monoglyceride as emulsifier at the concentration of 0.06%,0.05%,0.06%,0.30%,respectively.
  • Chen Juan, Kan Jian-quan, Zhang Rong, Tang Jun-ni
    Food and Fermentation Industries. 2011, 37(02): 113-119. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.038
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    The fundamental components of honey-mulberry wines with different mulberry varieties were analyzed,and the results were as follows: there were a few differences in the content of sugar,total acid,and alcohol,but many differences in protein content.The amount of each mineral element varied with different wines,while they all share the outstanding characteristic of high K and low Na,and Mg content was also high.With respect to the amount of total amino acid and essential amino acid,wine fermented from Hongguo1 mulberry were the highest,and wine fermented from agricultural mulberry were the lowest.Within seven phenolic acids and five flavonols determined,there were certain content differences among wines,while the content of protocatechuie acid、caffeic acid、quercetin and morin were higher and possessed by all wines.Furthermore,1-Butanol,3-methyl-,1-Propanol,2-methyl-,Benzeneethanol,Benzeneethanol,4-hydroxy-,2,3-Butyleneglycol,Ethyl acetate,Monoethyl succinate,S-Ethyl lactate,Acetic acid and Decanoic acid were basic aroma components of honey-mulberry wine.
  • Ma Rong-shan, Lu Yuan, Yan An-ting
    Food and Fermentation Industries. 2011, 37(02): 120-124. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.039
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    By determining the physical and chemical properties of high temperature Daqu and raw materials,we chose the appropriate ratio of the special accessories and obtained distillation processes fermentation product through two kinds of fermentations,solid state fermentation(SSF) and submerged fermentation(SMF).Main aroma components in fermented products were determined and quantified by gas chromatography.Eventually flavoring fermentation conditions for high temperature Daqu were confirmed by comparing the above results with that of the control experiment.
  • Zhu Dao-chen, Lv Zheng-feng, Liu Xing-rong, Wang Cheng-fu
    Food and Fermentation Industries. 2011, 37(02): 125-129. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.009
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    The recent advances in the mechanism of L-arabinose to inhibit the intestinal absorption of sugar have been reviewed.The overview on the application of L-arabinose in the different field,including food industry,medicine,and flavor industry was summarized.The potential values,various challenges,the opportunities,as well as future research directions are also presented.
  • Chen Hai-gui, Gu Nan, Liu Mei-yan, Zhao Guo-hua
    Food and Fermentation Industries. 2011, 37(02): 130-133. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.014
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    Biotransformation of citrus peels for making fuel ethanol production is a safe renewable method.With citrus peels as materials by enzymes or acid hydrolysing into fermentable sugar,microorganisms by the glycolytic pathway ferment sugar into ethanol,and extract concentration of 99.5% dehydrated ethanol which are fuel ethanol.This is designed to address a large number of by-products of citrus industry that caused environmental pollution,while producing ethanol as the depletion of oil energy alternatives.D-limonene inhibits microbial fermentation when biotransformation of citrus peels,but the reasonable control of various factors will reach a high ethanol production.Citrus peels by biotransformation for making fuel ethanol and affecting factors were reviewed,which would provide valuable references for utilization of citrus peels and bio-ethanol production.
  • Liu Mei-yan, Song Hui-ge, Chen Hai-gui, Zhao Guo-hua
    Food and Fermentation Industries. 2011, 37(02): 134-138. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.040
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    With the development of spectroscopic techniques,infrared spectroscopy,as a new means of testing geographical indication food,the role is becoming increasingly prominent.Compared with traditional methods,infrared spectroscopy technique has a long way to develop.Based on the introduction of geographical indication products and the principle of infrared spectroscopy technique,this article summarizes the application of near infrared spectroscopy,middle infrared spectroscopy and fourier transform infrared spectroscopy with chemometrics methods in testing geographical indication food such as wine,cheese,olive oil and so on,in order to provide basis for further research.
  • Qi Shi-peng, Xu Er-ni, Luo Yu-fen, Wu Xiao-dan
    Food and Fermentation Industries. 2011, 37(02): 139-143. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.005
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    High cell density culture refers to the simulation of artificial environment of human body in bioreactor for high-density cell growth and the yield of great amount of cells and their metabolites.This technique can reduce the cost of the equipment,production,shorten the production cycle,and dramatically improve productivity to meet the market demand.This paper reviews the application research of this technique and the research condition of the key culture methods.
  • Jiang Wei, Lan Yu-qian, Huang Yi, Xue Jie, Zhang Wu-jiu
    Food and Fermentation Industries. 2011, 37(02): 144-150. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.041
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    34 flavor compounds,including 8 alcohols,14 esters,4 aldehydes,5 acids,1 phenol,1 lactone,and 1 heterocycle,were determined in rice wine by solid-phase micro extraction and gas chromatograph-mass spectrometer.The precisions of the method ranged from 4.42% to 13.44%.The recoveries were 78.44%~118.92% with the detection limits in the range of 0.002~ 13.92 μg/L.All data above-mentioned demonstrated it was a good method.Rice wine with different ages determined by this method.The total content of flavor compounds ranged from 726.233 mg/L to 1290.769 mg/L.The main compounds in rice wine were alcohols,esters and aldehydes,accounting for 63.68%~ 71.10%,27.84%~ 34.09%,lower than 2%,respectively.Compounds with high content in rice wine in order were β-phenylethanol,ethyl lactate,iso-pentyl alcohol,isobutanol,ethyl acetate,diethyl succinate,2,3-butanediol and so on.The alcohols during aging decreased due to the oxidation,while the esters during the later aging increased because of the esterification and transesterification reaction.In addition,aldehydes during aging also increased by oxidation and maillard reaction.5-methyl-2-furaldehyde,furfural and benzaldehyde increased by 10~ 40 times during aging demonstrated those three compounds could be used as aging indicators of rice wine.
  • Li Fen-fen, Zhang Ben-shan
    Food and Fermentation Industries. 2011, 37(02): 151-154. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.042
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    The change of starch granule during gelatinization was determined by Focus Beam Reflectance Measurement(FBRM),which was an online monitor of the change of starch granule.Besides,a method to determine gelatinization degree by the total number of starch granules was proposed.The method simplifies previous method and provides useful reference model for the research of pasting property of starch.The results showed that: the number of corn starch measured by FBRM was first increased and then decreased,and the number decreased sharply at 70℃;the number of potato starch measured by FBRM showed a steadily decreased,and also had sharpest decline at 70℃;the number of tapioca starch measured by FBRM showed steadily decreasing with the sharpest decline at 65℃.The highest gelatinization degree for corn starch was 60.05% at70℃ and,potato starch was 38.83% at 70℃ and tapioca starch was 54.66% at 65℃;When the temperature reached 75℃,the total gelatinization degree of corn starch,potato starch and tapioca starch came to 93.55%,90.19% and 85.85%,respectively.None of them reached to 100%.
  • Chen Xun, Zhong Xiao-li, Liu Shu-liang
    Food and Fermentation Industries. 2011, 37(02): 155-159. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.043
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    According to the 16S rDNA and hip O gene sequences of C.jejuni in GenBank,two pairs of specific primers were designed and used in multiplex PCR for detection of C.jejuni in animal origin food.Multiplex PCR was developed and applied to the sample testing.The results indicated that two specific fragments(699bp and 366bp) were detected after amplification of the DNA template of C.jejuni,while other bacteria strains(11 species tested) were not detected.Meanwhile,the 16S rDNA and hip O gene sequence of C.jejuni ATCC33560 exhibited a high similarity with those of some strains of C.jejuni presented in GenBank.The total assay could be completed in 27h with a detection limit of 2.4~16CFU/mL.The chicken,pork,beef and milk from Ya'an markets in Sichuan province were detected by the multiplex PCR,and 38.0%,28.3%,17.1% and 8.6% of them were found respectively to be positive for C.jejuni.Multiplex PCR assay was specific,sensitive and time-saving,which provided reference for detection of C.jejuni in animal origin food.
  • Sun Xiao-xia, Zhao Zhan-min, Liu Dao-liang, Zhang Jun-feng
    Food and Fermentation Industries. 2011, 37(02): 160-164. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.044
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    In this research we distinguish the live cells,dead cells and other large particles accurately in the UHT milk samples using a new Fluorescence Labeling Technique basing on single live cell,and quickly detect microorganism quantitatively by using flow cytometry.Comparison with traditional plate counting method shows that the detection range of FCM is 101~107 CFU/mL,far above that of plate counting method.Correlation analysis of the results of the two methods shows that within certain concentration range of bacteria solution,the results of FCM and plate counting method are linear,and FCM is a more accurate and rapid detection method for different types of microorganism.
  • Chen Chang-song, Zhang Ying-gen, Zhong Qiu-sheng, Chen Rong-bing
    Food and Fermentation Industries. 2011, 37(02): 165-171. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.010
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    Aroma components of four Taiwang Oolong tea samples(Jinxuan Tea,Cuiyu tea,Ruanzhiwulong tea and Sijichun tea)which obtained from the same procedure of Taiwang Oolong tea manufacturing process were extracted by Simultaneous Distillation Extraction(SDE) and analyzed by gas chromatography-mass spectrometry(GC-MS).Comparision of the aroma components between the teas was conducted.And,the Result showed that the main kinds of aroma components in Oolong Tea were alcohols,esters and hydrocarbon compounds,but alcohols in Jinxuan Oolong tea was lower than in other teas.The major aroma compounds of Oolong tea are nerolidol,β-linalool,linalool oxides,phytol,α-farnesene,indole,hexadecanoic acid,hexadecanoic acid methyl ester,9,12-Octadecadienoic acid(Z,Z)-,methyl ester,9,12,15-Octadecatrienoic acid,methyl ester,2,2,4,6,6-pentamethyl-heptane,2,6-dimethyl-6-(4-methyl-3-pentenyl)-bicyclohept-2-ene,(Z,Z,Z)-,trienol,neophytadiene,docosane and pentacosane.By sensory evaluation,we found that the scores of Jinxuan,Cuiyu,Ruanzhiwulong and Sijichun Oolong tea were higher than Huangjingui(CK).Jinxuan Tea.And,the tasting quality of all the testing samples was higher than the control,even reached significant level.
  • Zhou Min, Yan Ting, Teng Jiu-wei, Tang Shu-ze
    Food and Fermentation Industries. 2011, 37(02): 172-175. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.011
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    Determination of Chromium(CrⅢ) in Drinking Water by Potassium bromide-Luminol-H2O2 Chemiluminescence System with Flow InjectionAbstract:Determination of Chromium(CrⅢ) in Drinking Water by Potassium bromide-Luminol-H2O2 Chemiluminescence System with Flow InjectionAbstract: Based on the obvious sensitization of potassium bromide on the chemiluminescent reaction in the system of luminol-H2O2–chromium(CrⅢ),a sensitive chemiluminescent method for determination of CrⅢ with flow injection technique was developed.The method is simple,rapid and effective to determine CrⅢ with the range of(1×10-6~1×10-3) mg/ mL and detection limit of 2.06 ×10-7 mg/ mL.The relative standard deviation(RSD) is 2.39% for the determination of 2.0 ×10-6 mg/mL of Cr(Ⅲ)(n=11).Ethylenediaminetetra acetic acid disodium salt(EDTA-2Na) is effectively eliminated the interference of some metal ions such as Fe2+,Fe3+,Cu2+,Zn2+.Therefore,it is a rapid method for determination of CrⅢ sudden changes in drinking water and a good monitor in giving an early alarm in the emergency contamination by heavy metals.The recoverie of CrⅢ was 91.7%~97.5%.
  • Yao Jun, Sun Rong-bin, Wei Qin-jun, Cao Xin, Lu Ya-jie
    Food and Fermentation Industries. 2011, 37(02): 176-178. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.026
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    Abstract The viscosity of γ-polyglutamic acid(γ-PGA) solution at different concentration was determined by ubbelohde viscometer.The intrinsic viscosity [η] of γ-PGA was 7.830 L/g calculated from Huggins & Kraemer equation.Meanwhile,the correlation of relative viscosity(ηr),specific viscosity(ηsp) and the γ-PGA concentration in aqueous solution was deduced.It was expected that the γ-PGA concentration could be facilely and precisely attained by the viscosity-assaying method.
  • Xu Li, Pan Li, Xuan Kai-zhi
    Food and Fermentation Industries. 2011, 37(02): 179-181. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.045
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    An ion chromatography method was developed for determination of galacto-oligosaccharide in syrup.The components of syrup was eluted with water,250 mmol/L sodium hydroxide solution and 1mol/L sodium acetate solution in gradient,and using the CarboPac PA10(2 mm×250 mm) column and amperometric detector.The method presented linear correlation coefficient between 0.999 0 and 0.999 7,relative standard deviation(RSD) 1.54 % and 3.42 %.The recoveries of the lactose in syrup sample ranged from 91.7 % to 5.7 %.The method has many advantages,such as simple operation,fast determination,accurate and highly sensitive.
  • Yang Jun, Wang Wen-hui, Li Yi, Wang Qi, Sun Xiao-dong, Zhu Yan-qin
    Food and Fermentation Industries. 2011, 37(02): 182-185. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.046
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    To establish an UPLC method in determination the amounts of Amino Acids in Walnuts.A Waters Acquity UPLC BEH C18 column(2.1 mm×150 mm,1.7μm)was applied,using AccQ.Tag Ultraa eluent A and AccQ.Tag Ultraa eluent B as the mobile phase,with a flow rate of 0.7 mL/min,and the column temperature 55℃.The calibration curve was linear in the range of 0.003~0.18 mg/mL of Amino Acids(r>0.999 4).The average recovery of Amino Acids was 98.5%.It has the advantage of simple,accuracy,and repeatable in the determination Amino Acids.
  • Wang Chun-xia, Lu Fu-ping, Liu Yi-han, Du Lian-xiang, Li Ming-run
    Food and Fermentation Industries. 2011, 37(02): 186-190. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.027
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    Nanofiltration was used in the extraction of cAMP from Jujube.Jujube was first soaked,then through puree,enzyme hydrolysis,filtration,resin adsorption and formic acid elution.The results showed: Nanofiltration fraction have a peak at a wavelength of 258nm by UV which was the same as the cyclic adenosine monophosphate(cAMP) standard;the Nanofiltration discard part has no peak at 258nm;HPLC showed that Nanofiltration interception fraction has the same retention time as the cyclic adenosine monophosphate(cAMP) reference,while the Nanofiltration discard liquid do not have the peak at the same time point.This indicated that nanofiltration intercepted the target product of cyclic adenosine monophosphate(cAMP),successfully separated molecular,purified and concentrated cAMP.
  • Wei Tao, He Pei-xin, Feng Sheng-xue, Wei Dong-zhi
    Food and Fermentation Industries. 2011, 37(02): 191-194. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.015
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    The ultrasonic-assisted extraction technology of crude polysaccharide from Achyranthes bidentata Blume and its antioxidant activities of the polysaccharide were investigated.On the basis of single factor test,extraction technology condition was optimized by orthogonal test.Our results showed the best extracting condition was as follows: ultrasonic power 1 000W,extracting temperature 60℃,extracting time 60 min,and material-water ratio 1∶ 30.Under the above conditions,the extraction rate of the crude polysaccharide can reach to 6.1%.Meanwhile,the scavenging activity of the polysaccharide on H2O2,O-2 · and ·OH free radicals were both significant.The clearance rates was up to 75.1%,90.3% and 61.3% respectively.
  • Qiao De-liang, Chen Nai-fu, Zhang Li, Chen Ke
    Food and Fermentation Industries. 2011, 37(02): 195-199. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.016
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    Polysaccharides from sporocarp of Agaricus bisporus was extracted by using methods of hot water lixiviation,ethanol precipitation,Sevag's deproteination and ethanol precipitation again.Extraction temperature,extraction time,ratio of water to raw material and extraction times were selected in single-factor tests.Based on the single-factor tests,combination of the extraction parameters was optimized by using four-factor-three-level orthogonal test.The optimum conditions were extracting temperature 80 ℃,extracting time 4 h,ratio of water to raw material 50 mL/g and extraction times 3.Practicing this optimal condition,extraction yield of polysaccharides from sporocarp of Agaricus bisporus was 3.510 5%.In crude polysaccharides of Agaricus bisporus sporocarp,carbohydrates content,determined by applying the phenol-sulfuric acid method,was 71.84%.Relative viscosity(to deionized water),detected by using Ubbelohde viscosimeter,was 1.109 6 at temperature of 25 ℃ and concentration of 10 mg/mL.In FTIR spectrum obtained from KBr pellet method,characteristic absorptions(3 700~3 100 cm-1 and 3 000~2 800 cm-1) of polysaccharides were observed clearly.
  • Liu Qi, Liu Ai-hong, Sun Mei-ling, Li Fu-jie
    Food and Fermentation Industries. 2011, 37(02): 200-204. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.017
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    Lactobacillus acidophilus,which is recognized as a safe fermentation creature,is probiotics which can stay in human intestinal wall.Many strains have been the stars in the food industry for a long time.The extro-polysacchrides of bacteria are macromolecule polymers which are secreted during the incubation or in the cell wall to protect the bacteria during incubation.The polysacchrides are diluted in the water.Its perfect characteristics in rheology and physics has put it on the stage for research and production.The extro-polysacchrides of lactobacii are not only important for flavor and quality of dairy,but also a secure source of polysaccharides,because of its capability of stabilizing and emulsifying the humidity of diary product.In this article,extraction of the extro-polysacchrides of lactobacillus acidophilus is optimized with the SAS software.The yield of polysaccharides is 1.91 g/L by extraction 80% ethanol(1:1.8) for 12hours.
  • Sun Zhao-yuan, Hou Hui-rong
    Food and Fermentation Industries. 2011, 37(02): 205-209. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.028
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    Studies were made on extracting polyphenol from pre-germinated brown rice.The effects of ultrasonic extraction time,ethanol concentration and proportion of material and solution on yield were discussed.Box-Behnken center-united experiment design and response surface methodology were used to optimize the extraction technology of polyphenol of pre-germinated brown rice.The optimal conditions of extraction were concluded as follows: ultrasonic extraction time 11.08min,ethanol concentration 80%,proportion of material and solution 1∶ 17.62.the actual detection value of polyphenol extraction rate was 60.13 μg/g,that is almost equal to the predictable value.
  • Liu Shu-xing, Hou Yi, Li Xiang, Liu Yu-cheng
    Food and Fermentation Industries. 2011, 37(02): 210-213. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.018
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    The produce technology of diosgenin extraction by sulfuric acid hydrolysis was studied with Dioscorea zingiberensis C H Wright as the raw material.Effects of sulfuric acid hydrolysis time,usage of sulfuric acid,Soxhlet extraction time and concentration of sulfuric acid on the sulfuric acid hydrolysis of dioscin were investigated.Optimized process by orthogonal experiments was obtained as: sulfuric acid hydrolysis 20mL for 4h,Soxhlet extraction 6h,sulfuric acid concentration at 1.5mol/L.Under the above conditions,the yield of diosgenin was up to 3.762%,acid pollution was reduced.The result provides a method to solve the existing problems in the diosgenin industrial.
  • Wang Hong-hai, Dai Zhi-yuan, Weng Li-ping, Yang Rong-hua, Zhang Yan-ping, Lu Yan-bin
    Food and Fermentation Industries. 2011, 37(02): 214-217. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.047
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    At different storage temperature,the smoked squid′s color change under vacuum packaging has been detected and analyzed by ColorQuest XE(chromatic meter).The results showed that the lower the storage temperature is lower,the little the color changed.All the color changes are happened during the early storage.No color changes if Semi-finished products stored at-18 ℃.Final smoked squid products also showed no obvious change at room temperature.
  • Xu Li, Yin Jing-yuan, Yao Liang-hui, He Hui-xia, Fang Yi, Gao Hai-yan
    Food and Fermentation Industries. 2011, 37(02): 218-223. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.012
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    The species of pathogen fungi of honey peaches("Hujinmilu" of Shanghai Nanhui District) as well as the effects of Thymol on the mycelial growth were investigated,and the effects of post-harvest treatment with modified atmosphere packaging and thymol on the physiology and fresh-keeping of honey peaches were studied under 3℃ storage.The results showed that the mainly pathogen fungi which caused the postharvest diseases of peaches were Alternaria,Monilinia fructicola,Cephalothecium,Rhizoctonia;and the thymol dramatically changed the physiological of the peach.It decreased the loss of fruit firmness and titrable acidity,increased membrane permeability.Thymol treatment significantly enhanced POD activity,and inhibited PPO activity.After 28 days,the good fruits rates was 79.1% by using 500 mg/L thymol.The results demonstrate that thymol treatment can increase the quality during storage.