SU Fei-ya,GU Xi-rong,DENG Xue-mei,LIU Yin-ni,ZENG Qing-ping,LIU Yan-jun,ZHAO Tian-qi
Food and Fermentation Industries. 2015, 41(5): 99.
In order to find an optimal medium to have more biomass and more mineral nutrition of Lactarius deliciosus in vitro,L9 (34) orthogonal experiments were arrayed on carbon sources,nitrogen sources and phosphorus concentrations.Results showed that nine media were significantly different on biomass and mineral nutrition content in L.deliciosus.Among the three factors,carbon sources have the most significant effect on the growth,the contents of K and Ca;nitrogen sources have the most significant effect on the contents of Fe and Mn;phosphorus concentrations have the most significant effect on the contents of P,Mg and Zn.Soluble starch was the best of carbon sources.Compared with glucose,soluble starch increased the biomass,the contents of P,K,Zn by 32.20％,16.94％,182.81％,and 59.09％,respectively.Among nitrogen sources,ammonium tartrate was the best on the growth,urea was the best on increasing the contents of P and K,and glutamic acid was the best on abosrbing Mg,Fe,Mn.Among phosphorus concentrations,0.5 g/L KH2PO4 was the best on the growth,P,K,Mg and Zn contents.The optimized medium for the growth and mineral nutrition of L.deliciosus was 18.0 g/L soluble starch,0.5 g/L ammonium tartrate and 0.5 g/L KH2PO4.
