25 March 2011, Volume 37 Issue 03
    

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    Food and Fermentation Industries
  • Feng Yong-wei, Xia Wen-shui, Zhang Wei
    Food and Fermentation Industries. 2011, 37(03): 1-5. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.022
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    An efficient procedure to prepare chitosan sorbate was established by using one two-step reaction.Firstly,sorbyl chloride was prepared by reflux of sorbic acid in thionyl chloride.Secondly,chitosan was selectively esterified with sorbyl chloride in MeSO3H.The chemical structure of the chitosan derivative was characterized by FT-IR and 1H-NMR analysis.The results showed sorbyl chloride mainly substituted with the hydroxyl group rather than amino group of chitsoan.The derivatives with DS of 0.44,1.21,1.93 were prepared with different molar ratio of chitosan and sorbyl chloride.The derivative(DS =1.21) manifested the strongest antimicrobial activity with the MIC of 0.125%,0.125% and 0.5(w/v) against Escherichia coli,Staphylococcus aureus and Aspergillus niger.
  • Xie Li-si, Zhang Hong-mei, Liu Xue-lu, Zhang Wen-yan, Huang Bao-wei, Xu Jia-jing, Zheng Tian-xin, Liu Yan-lan
    Food and Fermentation Industries. 2011, 37(03): 6-8. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.001
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    To isolate and identify lactic acid bacteria(LAB) from spoiled food and investigate the effect of different factors on biofilm formation.The strains were identified by the biochemistry methods.Biofilm formation was tested by microtiter-plate method under the different nutrient conditions,temperature and pH.The strains' biofilm was detected by microtiter-plate method.37℃,42℃and pH4 suboptimal for growth increased the production of biofilm.Low concentration of sodium chloride enhanced the biofilm formation(BF),but above a certain level,BFs were restrained.Different concentrations of glucose have different effect on strains' biofilm formation at different temperatures.BFs were spread widely among lactic acid bacteria from spoiled food.Preventing the formation of BFs has its significance in food preservation.
  • Lv Ning, Jin Feng-xie, Yu Hong-shan
    Food and Fermentation Industries. 2011, 37(03): 9-12. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.009
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    The Ophiopogon japonicus polysaccharide was hydrolyzed by bio-enzyme and the products were analyzed.The relative molecular weight of the Ophiopogon japonicus polysaccharide was 48347,which was purified by Sephadex G-100.The Ophiopogon japonicus polysaccharide was hydrolyzed by enzyme of Absidia sp.O8s,and the products were then isolated using Sephadex G-100 and Sephadex G-50 to generate three components: OP-1,OP-2 and OP-3.Their relative molecular weights were 32452,9231,1354,respectively.From HPLC,the purified Ophiopogon japonicus polysaccharide was mainly composed of glucose and fructose,then,OP-1,OP-2,OP-3 respectively consisted of fructose,fructose,glucose and fructose.
  • Zhang Bo, Yin Yan
    Food and Fermentation Industries. 2011, 37(03): 13-17. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.002
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    A xylose isomerase produced by thermophilic bacterium Anoxybacillus flavithermus was purified using Sephadex G-75 gel filtration,DEAE-cellulose anion exchange chromatography and Q Sepharose Fast Flow anion exchange chromatography.The estimated molecular mass of the purified xylose isomerase was 181 ku and was made up of four sub-groups.The optima temperature and pH of enzymatic reaction were 80°C and pH 7.0,respectively.This xylose isomerase is alkali-tolerant and thermo-stable: the enzyme activity keep about 80% in 70℃ for 1h and keep about 80% in pH5.0~8.0 for 1 h,even keep about 40% in pH12.0 for 1 h.The enzyme was mainly activated by Mn2+ and Co2+ but was mainly restrained by Zn2+,Cu2+ and Al3+.
  • Wang Li, Zhu Li-dan, Sheng Hui-ming, Chen Zheng-xing
    Food and Fermentation Industries. 2011, 37(03): 18-22. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.010
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    A novel sulfated polysaccharide SRBPS2a with anti-tumor activity was derived from defatted rice bran by chlorosulfonic acid-pyridine(CSA-Pyr) method.The average molecular weight of SRBPS2a was 3.5×105 u and the degree of sulfation(DS) was 1.29.The Fourier-Transform Infrared Spectra(FT-IR) and 13C NMR spectroscopy analysis revealed that SRBPS2a was mainly consist of β-(1→3)-D-galactopyranosyl residues,the sulfate substitution site was on C-2 and C-4 while the side chains were cut off during the sulfated reaction.Furthermore,SRBPS2a exhibited evident growth inhibition on mouse mammary tumor EMT-6 cells both in vitro and in vivo.
  • Yin Feng, Zhou Ai-mei, Zhang Xiang-gang, Cao Yong, Liu Xin, Chen Yong-quan
    Food and Fermentation Industries. 2011, 37(03): 23-26. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.004
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    With hydrolysis test as an orientation,the major autoenzyme was purified from the head of white shrimp(Penaeus vannamei) by sulfate precipitation,gel filtration on Sephadex G-100 followed by ion-exchange chromatography DEAE-DE52.The autoenzyme was then identified by inhibitor tests and SDS-PAGE analysis.The results showed the major autoenzyme from the head of white shrimp had a specific activity of 2.2×106 U/g with a 96.90-fold increase over that of the crude enzyme extract.This autoenzyme was inhibited by serine protease inhibitor phenyl methyl sulfonyl fluoride(PMSF) with the inhibition rate being 91.3%,and illustrated as a single band in SDS-PAGE with the same distance as that of standard trypsin.These results indicated that trypsin was the major autoenzyme in the head of white shrimp.
  • Lin Shao-ling, Tang Shu-ze, Tang Shu-shu, Wu Xi-yang, Chen Zhen-qiang
    Food and Fermentation Industries. 2011, 37(03): 27-31. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.003
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    Anti-microbial photodynamic technology(APDT) on L.monocytogenes was conducted using photo sensitizer hematoporyrin monomethyl ether(HMME) followed by tungsten-halogen lamp(power density 200 mW/cm2).The bactericidal effects were measured by counting the reduction of colony forming unit(CFU) after APDT-HMME treatment.The degradation of protein was observed under sodium dodecyl sulphate polyacrylamide gel electrophoresis(SDS-PAGE),and the damage of DNA was investigated by polymerase chain reaction(PCR).Approximately 99.9999% of L.monocytogenes were photo inactivated by illumination with 30 min visible light in the presence of 25 μg/mL HMME.The cell killing effect to L.monocytogenes with illumination was much greater than in the dark at the same concentration of HMME.The protein of L.monocytogeneirradiated with HMME and visible light for 30 min was degraded obviously shown by SDS-PAGE graph and the DNA damage shown by PCR graph was greater in APDT-treated groups compared with the control groups.The significant cell killing effect of APDT-HMME on L.monocytogenes may be explained by the bactericidal mechanism through the degradation of the protein and the damage of DNA.
  • Yan Liang-zhen, Li Xiao-ran, Quan Zhe-xue, Ma En-bo
    Food and Fermentation Industries. 2011, 37(03): 32-36. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.023
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    In this paper,different Fenjiu fermentation samples,Fen Daqu starter and environment samples were used for DNA extraction.Two different methods,the enzymatic-physical-chemical method and physical-chemical method,were implemented to accomplish the stated goal,and the former method led to quality DNA with high purity.The follow-up experiment showed that the DNA obtained through the enzymatic-physical-chemical method allowed full reflection of the diversity of the microbial community from every different sample during the production and fermentation of Fenjiu.
  • Liang Zhi-zhou, Xu Min-jun, Hu Hai-yan, Zhou Shi-ning
    Food and Fermentation Industries. 2011, 37(03): 37-40. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.024
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    The production of red pigment was greatly improved by Monascus incubated with Saccharomyces cerevisiae lysate.Yeast lysate was added at different time points to the different flasks which contained media inoculated with Monascus and the difference of the red pigment yield was investigated.The effect of the amount of the yeast lysate added on the red pigment yield was also analyzed.The results shows that 48 hours after inoculating with Monascus is the most suitable time point to add yeast lysate and 1.5 mL is the most suitable amount of yeast lysae for 150 mL culture mix to increase the pigment yield.Under the most suitable condition mentioned above,the rates of increase in yield of Monacus mycelium,hydrophobic pigment and hydrophilic pigment were 47%,120% and 190%,respectively,as compared with control.
  • Li Li, Gu Xin, Cui Jie, Meng A-hui, Hou Ya-kun, Wang Jian-zhong
    Food and Fermentation Industries. 2011, 37(03): 41-45. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.011
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    The antioxidant activity of the brown pigment extracted from chestnut shells by two different solutions was compared by IR spectra.The extraction was done by 1% sodium hydroxide and 40% ethyl alcohol respectively.The evaluation was measured by the total antioxidant activity,DPPH radical scavenging ability,hydroxyl radical scavenging capability and superoxide radical scavenging capability.The results show that the samples antioxidant activity increased with the amount of the pigment.Compared with Vc and BHT,Vitamin C showed the highest antioxidant activity.The second is brown pigment extracted by ethyl alcohol.The brown pigment extracted with sodium hydroxide is close to the BHT.The order for DPPH radical scavenging ability is:Vitamin C,brown pigment extracted with ethyl alcohol,brown pigment extracted with sodium hydroxide and BHT.The IC50 of the brown pigment extracted with ethyl alcohol is about 0.02 mg/mL.The IC50 of the brown pigment extracted with sodium hydroxide is about 0.09 mg/mL.For hydroxyl radical scavenging capability,the samples exhibit higher scavenging capability with the increasing of the amount of the samples and the four samples were all very close.The superoxide radical scavenging capability result showed that alkali extraction IC50 was 0.71 mg/mL,the alcohol extraction is about 0.83 mg/mL,both weaker than Vitamin C and BHT.The IR spectra showed the difference between two method extracting pigments.
  • Yang Xing, Zeng Xin'an
    Food and Fermentation Industries. 2011, 37(03): 46-49. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.025
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    The interaction between hydrogen bonding in 40% and 60% volume fraction of ethanol-water spirit simulation and temperature ranging from 18℃ to 58℃ was investigated by the application of 1H NMR.It was found that not only the strength of hydrogen bonding weakened but also the varying frequency of associative structure and proton exchange speeded up with the temperature increasing.Furthermore,it was testified there was a linear relationship between temperature and the chemical shifts of hydroxyl in related solution,which proved that strong hydrogen bonding predominate in this system.The related concludes partly demonstrated that temperature change could have an effect on the taste of spirit and provided some data support for the process of wine production from spectrum analysis.
  • Kong Bao-hua, Li Ming-qing, Xia Xiu-fang
    Food and Fermentation Industries. 2011, 37(03): 50-55. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.026
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    Common carp(Cyprinus carpio) myofibrillar proteins are essential to the surimi products,and the influence of NaCl,CaCl2 and Na4P2O7 on myofibrillar proteins were examined.The isoelectric point carp myofibrillar proteins was 5.5.Salts effect on the carp myofibrillar proteins of molecular weight was not obvious in the SDS-PAGE,while with the increasing concentration of NaCl,CaCl2 and Na4P2O7,myofibrillar protein gel hardness,springiness,turbidity and surface hydrophobicity were all reduced.NaCl and Na4P2O7 modified the microstructure of myofibrillar gel,but CaCl2 caused gel microstructure to loose.Protein gel with CaCl2 salt solution had lower surimi gel hardness,flexibility and turbidity than others,and the difference was significant(P<0.05).The structure and gel properties of carp myofibrillar protein were changed when protein dissolved in salt(NaCl,CaCl2 and Na4P2O7) solutions.
  • Yin Chang-lin, Liu Jia, Zhao Zheng
    Food and Fermentation Industries. 2011, 37(03): 56-60. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.027
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    The experiment studied the influence of pH on the functional properties of processed cheese by using texture analyzer,rheometer and SEM,and it described that pH played an important role in the process of making processed cheese.The results indicated: pH influenced the meltability of processed cheese,but the results were not regular.With increasing of pH,the apparent viscosity of processed cheese increased.Among all the parameters of processed cheese's texture,both the hardness and chewiness declined as pH increased in processed cheese,springiness were influenced significantly by pH(P<0.05);pH affected the process of emulsification,emulsification became better at higher pH value.
  • Zheng Shuang, Zhu Xin-gui, Zeng Xiao-bo
    Food and Fermentation Industries. 2011, 37(03): 61-64. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.028
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    The soy sauce was separated by freezing.The freezing point of 35% soluble solid soy sauce was-20.3℃,and it was frozen at-30℃ for 5h.At the same time the physical and chemical indicators of the components was measured in two-phase soy sauce.The result showed that the concentration of some components have increased in two-phase as the temperature decreased.The mass components in ice phase increased as the temperature decreased but the mass distribution in liquid was on the contrary.The concentration of amino acid nitrogen which was the most important indicator increased from 0.88g/100mL to 1.168g/100mL.The distribution coefficient showed that amino acid nitrogen was easier concentrated in the liquid phase with the temperature decreased.The further study showed that amino nitrogen component Glutamic acid,Leucine,Aspartic acid were all more easily concentrated in liquid phase.
  • Pan Chun-mei, Xing Yan, Fan Yao-ting
    Food and Fermentation Industries. 2011, 37(03): 65-69. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.016
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    A two-phase process combined production of cellulosic ethanol and hydrogen from cornstalk was constructed in this paper.The effect of detoxification methods on the production of ethanol and hydrogen from acid-hydrolysis cornstalk were investigated.The results showed that using anion-exchange resins detoxification followed by evaporation,the maximal ethanol and hydrogen yields from cornstalk were 131.1 mg/g TS and 44.6 mL/g TS,respectively.Using acid hydrolysis method,the hemicellulose in cornstalk was degraded for ethanol production.The natural hydrogen-producing microorganism had the ability to directly degrade cellulose for hydrogen fermentation.
  • Feng Chong, Geng Tao, Wang Yan-ying, Liu Yan-li, Song An-dong
    Food and Fermentation Industries. 2011, 37(03): 70-74. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.015
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    Trichosporon fermentans(TF)was domesticated to improve adaptability in fiber hydrolysate.Fermentation system from fiber hydrolysate by the domesticated TF have been optimized.By the flask experiments of several single conditions which affect TF growth and fat synthesis,the optimal fermentation conditions were studied.After domestication,the transform rate of sugar increase 37.2%,the biomass increase 24.3%,lipid content increase18.3% and lipid concentration increase 45.6%.The optimum fermentation conditions which were determined on the basis of a single test to be as follows:concentration of sugar,60~80 g/L,pH 6.0,quality ratio of yeast +(NH4)2SO4 3∶1.On the optimum condition,the biomass,lipid content and lipid concentration is 22.6 g/L,46.3%,and 10.5 g/L,respectively.At the same time,the composition of bio-oil was analyzed.
  • Zhao Jia-min, Chen Zhong, Lin Wei-feng
    Food and Fermentation Industries. 2011, 37(03): 75-78. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.029
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    In this study,food-grade microemulsions were prepared with tween-80 as the surfactant phase(S),ethanol,propylene glycol and glycerol as the co-surfactant phase(CS) and soybean oil,medium chain triglycerides and ethyl butyrate as the oil phase(O).Stability of microemulsions was evaluated by particle size determination and visual observation of microemulsions affected by environment factors,including centrifugal effect,temperature,salinity and pH acidity.Stability comparison of different microemulsions was also estimated with the same method.The results indicated that stability of microemulsions was pretty good and microemulsions were thermostable systems.Stability of the microemulsion system composed of tween-80 as the surfactant phase,ethanol as the co-surfactant phase and ethyl butyrate as the oil phase was best.
  • Liu Hai-ying, He Lei, Guo Shi-dong
    Food and Fermentation Industries. 2011, 37(03): 79-83. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.030
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    In this study,channel catfish skins were dried with three different methods: air dry at room temperature,hot air dry and marinated with salt.The treated samples were preserved at room temperature for 10 days,30 days,60 days and 90 days.Channel catfish skin shrinkage temperature were measured by differential calorimetry(DSC) method.Collagens of those fish skins were extracted.The molecular weight distributions were determined by SDS-PAGE,and the Fourier Transform Infrared Spectroscopies of the collagens were also studied.The results showed that natural drying was the best treatment for fish skin preservation.
  • Jiang Hui-yan, Sun Pei-long, Shao Ping
    Food and Fermentation Industries. 2011, 37(03): 84-89. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.017
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    Radial flow chromatography(RFC) was a new technique of chromatography purification,and has a great potential in biotechnology.The deproteinization and decoloration of polysaccharide from Ganoderma lucidium by radial flow chromatography were studied.The optimal conditions were determined by single factor experiments and response surface experiments: sample concentration 10 mg/mL,sample volume 100 mL,sample flow-rate 2 mL/min and elution flow-rate 40 mL/min.The deproteinization rate,decoloration rate and polysaccharide recovery rate under the optional chromatography condition were 80.35%,88.52% and 85.06%,respectively.The purification efficiency of RFC was compared with sevag method,trichloroacetic acid method,activated carbon method and hydrogen perioxide method.The comparative results indicated that the purification by RFC were superior to other methods.None organic reagent in RFC treatment shows its advantages of high-efficiency,more economic,safe,and environmental friendly.
  • Wei Jue, Wan Ping, Qin Fang, Xu Jie, Zhao Xiao-Long, Guo Yu-rong
    Food and Fermentation Industries. 2011, 37(03): 90-93. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.031
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    Optimization on the Process of Chemical Modification of Apple Dietary Fiber through Orthogonal Regression Design.Technology of modification of apple dietary fiber by acid,alkali process was optimized with orthogonal regression design.The orthogonal models were established to express the relationship between temperature(X1),pH(X2),time(X3) as shown in the acid and alkali process equations:y^=-5.907+0.174X1-0.377X2+0.946,y^=3.698-0.113X1+2.816X2-1.093X3.According to these models the best condition for acid method was 96℃,3h,in pH3.7,while the alkali method was at 63℃,0.4h at pH11.6.The highest yield of SDF by acid,alkali process was 12.2% and 28.8%,respectively.Experiment results were similar with theoretical prediction of 11.8% and 28.1%.The factor order on SDF yield by acid,alkali process was temperature>time>pH,pH> time>temperature respectively.Under the modified acid,alkali process,the average water holding capacity increased 363.1%,236.4% and expansibility of SDF increased 48.2% and 52.8% compared with previous method.
  • Jiao Long, Yu Hong-wei, Guo Run-fang, Bai Yu, Jia Ying-min
    Food and Fermentation Industries. 2011, 37(03): 94-98. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.018
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    The fermentation medium and conditions of the strain were studied via single factor test and orthogonal design.The optimal fermentation medium was determined as follows:glucose 1.5%,soypeptone 2.0%,KH2PO4 0.5%,MgSO4·7H2O 0.05%.The best fermentation conditions were:inoculating time 14 h,inoculating amount 1%,methanol supply 1.5%,medium volume 30 mL in 250 mL flask,initial pH 5.5.Under such conditions,the activity of fibrinolytic enzyme(corresponding to the unit of urokinase activity) was 429.06 U/mL,which was 8.88 times of the initial strain.
  • Zhang Min, Zhang Jin-ze, Duan Su-fang, Gao Jia-tao, Wang Xue
    Food and Fermentation Industries. 2011, 37(03): 99-103. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.019
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    By using Plackett-Burman and response surface methodology,we studied the medium optimization of purification of stachyose by fermentation.A Plackett-Burman design was first used to evaluate the influence of six related factors,and yeast extract,casein peptone,NaNO3 were affirmed the important factors in the medium,and then the path of steepest ascent and the Box-Behnken design were used for further optimization on these three factors.By solving the quardratic regression model equation,the optimal concentrations of the variables were determined as: yeast extract1.38%,casein peptone 0.82%,NaNO30.48%.Under the optimal culture condition,the purity of the stachyose increased from 85% to 91%.
  • Liu Quan-de, Liu En-qi, He Ju-ping, Zhang Jian-ping
    Food and Fermentation Industries. 2011, 37(03): 104-108. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.032
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    This study took the content of amino nitrogen as evaluation indicator,combined sensory scoring,and determined the optimum technology of primary fermentation following these steps: added 3% rice flour in cooked soybean,inoculated 3% lactic acid bacteria,fermented for 2.5 hours at 42℃(pH 5.0),then fermented further for 18h at 37℃ with additional 5% mix-culture of rhizopus oligosporus and sweet wine koji(ratio of 3∶2).The results showed that the contents of amino nitrogen were almost the same as the former fermented soybean fermented in 5% saline at 20℃ for 9 days,2% saline at 4℃ for 15 days after post-fermentation maturing.Nutritional facts and flavor of tempe were both improved after fermentation.By freeze-drying or frying,tempe can be developed as snack food,fermented seasoning and table food.
  • Zheng Lin, You Li-jun, Zhao Mou-ming
    Food and Fermentation Industries. 2011, 37(03): 109-112. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.033
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    The effect of pasteurization,boiling sterilization and high-pressure steam sterilization on the antioxidant activity of loach peptide were investigated.The results showed that the absorbance of loach peptide at 420 nm increased significantly,which was treated by boiling sterilization or high-pressure steam sterilization.Under this condition,the loach peptide could maintain a high ORAC value.Both the DPPH free radical scavenging activity and the reducing power of loach peptide decreased after thermal sterilization treatment,while high-pressure steam sterilization had the least influence on it.Pasteurization and high-pressure steam sterilization could enhance the Cu(Ⅱ) ion chelating activity of loach peptide.Based on the above results,we can conclude that loach peptide had good heat resistance and it could maintain stronger antioxidant activity by high-pressure steam sterilization,which may be related to the Maillard reaction.
  • Zhuang Hui-ting, Du Jin-hua, Guo Chun-bao, Wang Cong
    Food and Fermentation Industries. 2011, 37(03): 113-117. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.034
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    The changes of color during wine fermentation were affected by many factors.In this paper,three pomegranate(Punica granarum L.)cultivars named qingpi sweet,qingpi sour and taishan red were selected to study anthocyanins,chrome,tone changes during fermentation and compare color between pomegranate juices and pomegranate wines.The results showed that the maximum scanning wavelength of qingpi sweet,qingpi sour and taishan red pomegranate juice were 513nm,496 nm,514 nm respectively.Anthocyanins,chrome,tone of pomegranate juices were decreased at some degree during fermentation.Taishan red had highest anthocyanins content 82.26 mg/L,while,at the end of fermentation the anthocyanins content was 50.70 mg/L,we could see that there was significant difference between three pomegranate cultivars about the anthocyanins content(P< 0.01).When fermentation finished,the chrome value of qingpi sour(SO2 added was 1.323,the other was 1.328) were higher than qingpi sweet and taishan red,there was significant difference between qingpi sour and qingpi sweet,taishan red about chroma value(P< 0.01).At the same time,the tone value of qingpi sour and taishan red were higher,there was no significant difference between two cultivars about chrome value(P>0.05),but had significant difference between qingpi sweet(P< 0.01).In the study,we added 50mg/L SO2,but we found that during fermentation whether added SO2 or not had no significant difference on anthocyanins,chrome and tone value(P>0.05).Also,through correlation analysis between anthocyanins,chrome and tone,we concluded that there had no significant correlation between anthocyanins and chrome(Pearson correlation coefficient was 0.199,P=0.608>0.05),the regression equation was y=0.0029x+1.0905;while,there had significant correlation between anthocyanins and tone(Pearson correlation coefficient was 0.885,P=0.002<0.01),the regression equation was y=0.0029x+1.0905.
  • Liu Kun, Gao Ting-ting, Yang Liu
    Food and Fermentation Industries. 2011, 37(03): 118-121. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.035
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    The clarification of grape juice by means of chitosan was studied.The results from the test of single factor showed that the optimum chitosan amount,temperature,pH and clarification time was 0.6~0.8g/L,50~70℃,3.49 and 40~60min respectively for clarification of grape juice.The results from the orthogonal test showed that the optimum technological condition for clarification of grape juice was adding 0.8 g/L chitosan at 60℃for 50min and its transmittance of the clarified grape juice was up to 92.3%.Comparing with the original grape juice,the contents of soluble solids,pH were almost the same after clarification.Removing both the pectin and some proteins improved the stability of grape juice.
  • Wang Wen-xia, Song Chun-li, Zhang Xiao-jing, Wang Wei, Wang Li
    Food and Fermentation Industries. 2011, 37(03): 122-125. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.036
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    Soluble dietary fiber was obtained from insoluble dietary fiber of corn bran by treatment with a celluclast.Insoluble dietary fiber is subjected to a sodium hexametaphosphate solution or high pressure treatment in order to enhance enzymic access and soluble dietary fiber production.The results showed that high pressure treatment was most useful to increase the yield of soluble dietary fiber,and high pressure treatment condition was 121℃,3 h.The reaction parameters affecting the soluble dietary fiber,such as substrate concentration,enzyme loading,pH,reaction temperature and reaction time,have been investigated.The maximum soluble dietary fiber production was observed after 11 h incubation of enzyme loading 2%,substrate concentration 40 g/L,temperature 55 ℃,and pH 4.0.The obtained yield of soluble dietary fiber was 10.37%.
  • Huang Li
    Food and Fermentation Industries. 2011, 37(03): 126-129. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.037
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    With chayote as raw material,and glucose,jelly powder and citric acid as assistant material,the optimum processing technology and material formula was studied to develop chayote health jelly without any preservative and colorant.The final product was analyzed by sensory,chemical and microbiological inspection.Results showed that the optimum processing technology was that,choose glucose as sweet agent,the chayote juice temperature when adding jelly powder was 50℃,the continual boiling time was 1min,citric acid was added when pressing juice;the optimum material formula was that chayote juice 53.4%,water 30.5%,glucose 15.2%,citric acid 0.1%,jelly powder 0.8%.The above method could produce chayote health jelly with reseda,homogeneous and exquisite organization,delicious taste and good elasticity,rich nutrition and high edible safety.
  • Zhang Yu, Miao Ming, Jiang Bo, Zhang Tao
    Food and Fermentation Industries. 2011, 37(03): 130-135. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.038
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    As a novel non-thermal processing technology,high pressure processing(HPP)has been a study hotspot of manufacturing field that develop the modern healthy nutritional foods.This study briefly introduces the influence mechanisms of HPP upon the enzymes and basic research at home and abroad in resent years,and reviews the process of the catalytic characteristics of various food enzymes under the treatment of high pressure,and finally forecastes the development prospect.
  • Liu Zhong-yong, Wei Xiao-qun, Xie Li
    Food and Fermentation Industries. 2011, 37(03): 136-140. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.039
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    Currently,Codex Alimentarius Commission,European Union,American,Canada and Argentina published their act or standards related to "gluten free" foods labeling for protecting persons intolerant to Gluten.In this article,the definition,the requirement of labeling,quality controlling and the allergen inspection methods of gluten free food were introduced,followed by the countermeasure considering the management and development of gluten free food industry in China.
  • Lu Hong-jia, Zheng Long-hui, Liu Xiong
    Food and Fermentation Industries. 2011, 37(03): 141-145. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.040
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    Microcrystalline cellulose(MCC) is produced by acid hydrolysis of natural cellulose to a level off degrees of polymerization(LODP).It had wide applications in food,pharmaceuticals,and other industries.This paper mainly reviews its physicochemical properties,its application in food industry,and the prospect of its future development.It also provides theoretical references for researchers for further in-depth study.
  • Hu Xiao-liang, Zhou Guo-yan
    Food and Fermentation Industries. 2011, 37(03): 146-150. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.005
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    Chitosan,the only biological large existence polysaccharides,non-toxic and odorless,biodegradable,biocompatible,low cost,excellent dispersion,moisturizing,film forming,antimicrobial,is becoming a hot research topic on preservation of fruits and vegetables.The mechanism of chitosan and its derivatives on fresh keeping fruits and vegetables are introduced.The application of chitosan and its derivatives for the preservation of fruits and vegetables are reviewed.In addition,some suggestions of the development are also presented.
  • Peng Zhen, Zhao Guo-hua
    Food and Fermentation Industries. 2011, 37(03): 151-157. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.041
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    Recently,ultra-high pressure processing has been introduced and suggested as a food preservation technology,and it could maintain the nutritional attributes and authentic sensory properties.However,some studies have shown that as a result of the strong mechanical force formed during the high-pressure treatment,the reducing of volume and increasing of temperature of the system may have impact on the structure and permeability of the plastic packaging films,and then affect the self-life and quality of the packaged food.In the light of published literatures,the effect of UHP on structure and physicochemical properties of plastic packaging films will be reviewed.
  • Yang Xiao, Rui Guang-wei, Zhong Zhi-chao, Gan Ping, Sheng Jia-wu
    Food and Fermentation Industries. 2011, 37(03): 158-162. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.006
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    Nine species Zanthoxylum bungeanum maxim of different habitat and grade from Sichuan and Chongqing were used as raw materials.Aroma constituents and tingling principle extracted by the method of supercritical CO2 simultaneously were analyzed by GC/MS/AMDIS.There were total 66 chemical components identified from extractant of Zanthoxylum bungeanum maxim,and the content of aroma constituents and tingling principle differed in Zanthoxylum bungeanum maxim by varieties of habitat and grade.The content of major aroma constituents including linalool,α-terpineol,1,8-cineole,myrcene,and limonene was higher in Zanthoxylum schinifolium(green huajiao) than Zanthoxylum bungeanum(red huajiao).The content of tingling principle constituted of alkylamide was higher in Zanthoxylum bungeanum(red huajiao) than Zanthoxylum schinifolium(green huajiao).
  • Xu Chun-hua, Xiao Zuo-bing, Niu Yun-wei, Yu Hai-yan
    Food and Fermentation Industries. 2011, 37(03): 163-167. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.042
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    Comparison analysis of smell and taste between cherry wine(dry red & dry white) and grape wine(ChangCheng dry red wine & Changyu dry white wine) was conducted using an electronic nose,an electronic tongue and sensory evaluation.Principal component analysis(PCA) and discriminant factorial analysis(DFA) were used for data analysis.The results of pattern recognition showed that the aroma between dry white cherry wine and dry white grape wine had little differences,but the taste difference between cherry wine and grape wine was significant.The results of electronic nose and electronic tongue were in accordance with that of sensory analysis.The conclusion is that electronic nose together with electronic tongue with pattern recognition fit for flavor analysis of fruit wine.
  • Ju Yun-dong, Du Jin-hua, Wang Xiu-ju, He Gui-fen
    Food and Fermentation Industries. 2011, 37(03): 168-171. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.043
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    The critical components of hop pellets of three cultivars,Qingdao,Zhayi,Makeboluo were determined and the potential value in beer brewing and storage characteristics of them were studied.The results showed that HSI value of all the pellet products was higher than 0.35;the content of hop oil and α-acid of Makeboluo was the highest in three with the amount as 1.04 mL/100 g and 9.67%±0.15% respectively.However,Zhayi was considered to have the highest brewing value in accordance with the ratio of humulene to carypohyllene,myrcene to the sum of carypohyllene and humulene,and α-acid to β-acid,while the brewing value of Makeboluo was the lowest in three.With nitrogen gas packing,α-acid and hop oil of the pellets lost gradually during storage and the loss rate decreased.β-acid,water,and total phenol content had no significant changing trend.
  • Jiang Bao, Zhang Zhen-wen, Wang Li-na, Liu Ling, Guo Yan
    Food and Fermentation Industries. 2011, 37(03): 172-176. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.044
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    Chemical constituents of the volatile compounds from the Cabernet sauvignon,Cabernet gernischt,Chardonnay varieties of wine-grape berries from xiangning County Shanxi province of Loess Plateau region were analyzed and compared by headspace solid-phase micro-extraction(HS-SPME) followed by gas chromatography-mass spectrometry(GC-MS).The result showed 24,20,23 and 27 compounds from Cabernet sauvignon of slope land,Cabernet sauvignon of flat land,Cabernet gernischt and Chardonnay varieties grapes.These volatile compounds belong to five classes whereby their contents decrease in descending order: alcohol,aldehyde,acid and cyclic olefin;as far as single compound concerned,the contents of 1-butanol,hexanal,acetic acid and hexanoic acid were higher.Besides,terrains of vineyards could indeed affect the composition and content of the volatile compounds,although their aromatic profiles were basically consistent.
  • Zhou Ning, Liu Bao-lin, Wang Xin, Wang Hong-zhi, Yang Pei-qiang, Zhou Hang
    Food and Fermentation Industries. 2011, 37(03): 177-181. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.020
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    The relaxation maps of three edible vegetable oils(peanut oil,sunflower seed oil and sunflower seed oil) and rice bran crude oil were detected by low-field nuclear magnetic resonance technology(NMR).An obvious characteristic peak has been determined in the relaxation maps of rice bran crude oil at 10ms,which can not be detected in the other three kinds of pure edible oil.And the area proportion increased with the increase of rice bran crude oil content.Therefore,the detecting of the characteristic peak area can judge the vegetable oil adulterated with rice bran crude oil.
  • Li Fei-yan, Liang Rong-rong, Zhang Yi-min, Luo Xin
    Food and Fermentation Industries. 2011, 37(03): 182-186. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.045
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    In order to provide the basis for improving the quality of beef,the changes of quality indexes on chilled beef during storage were analyzed and the correlations between physicochemical properties and microbial indexes were investigated.The changes of quality indexes including total plate count(TPC),psychrophilic count,pH values,total volatile basic nitrogen(TVB-N),water holding capacity,muscle color and the correlations of chilled beef during 4℃ were studied.The experiments were carried out at 0,2,4,6,8,10,12 and 14 days of storage.The results showed that during storage,the pH was first declined and then raised,TVBN increased,the water holding capacity and the redness a* value and DE(difference error) values of beef were gradually declined with the preservation time.There was a positive correlation between the logarithm of total number of microorganisms and psychrophilic count and the pH value with a significant difference(P<0.05),while the logarithm of total number of micro-organisms was negative correlated with the redness of chromatic aberration and the water holding capacity(P<0.05).TVBN and redness a* value showed the best correlation with TPC.Therefore,microorganism's activities can affect the changes of physicochemical index.TVBN and redness a* value could potentially be used as indicators in predicting the microbial quality of chilled beef objectively.
  • Li Xiao-ping, Chen Jin-ping, Zhang Fu-xin
    Food and Fermentation Industries. 2011, 37(03): 187-190. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.046
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    The effect of tea polyphenols(TP) on color stability and lipid oxidation of ostrich meats with vacuum-package and(4±1)℃ storage was investigated.TP-treated groups can consistently restrain the lipid oxidation of ostrich meat.TP at levels of 200 mg/kg(T200) and 400 mg/kg(T400) can decrease the form of metmyoglobin and improve the content of total pigment and the color a*.The results obtained showed that TP not only improved lipid stability but also improved color stability in ostrich meat.
  • Zhu Jing, Shi Jun-ling, Liu Yan-lin
    Food and Fermentation Industries. 2011, 37(03): 191-196. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.047
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    In this paper,different extracting methods of Hexanol degradating enzyme from apple were investigated for purpose of comparison,and the best extracting method was obtained.Then crude enzyme extracted with above suitable method was separated and purified for further purification.Results showed that: enzyme extraction with acetone was the best;the SDS-PAGE indicated the electrophoretic bands and molecular weight(MW) of the enzyme obtained through different measures were different from each other.The suitable concentration for ammonium sulphate fractional salting out is 30%~70%,and the Hexanol conversion rate is up to(88.56±0.984 7) % by means of gradient salting out.
  • Yang Qing-li, Qin Song
    Food and Fermentation Industries. 2011, 37(03): 197-201. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.021
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    Kosteletzkya virginica seeds were taken as raw materials and the aqueous enzymatic extraction technology was used to extract seed oil.The single factor experiment and the response surface methodology were adopted to study the effects of solid to water ratio,extraction temperature,the ratio of enzyme to seeds,extraction time and pH on oil extraction rate.The order of these factors in terms of the influence on the oil extraction rate within the experimental range was as follows: the ratio of enzyme to seeds>extraction temperature >the ratio of solid to water>extraction time.The optimal technological parameters of oil extraction from Kosteletzkya virginica seeds should be as follows: the ratio of enzyme to seeds 0.024 mL/g,extraction temperature 63℃,the ratio of solid to water 1/6 and extraction time 230 min.Under such conditions,total oil extraction rate is 24.281%.
  • Jiang Zhi-guo, Chen Wen-xue, Liu Si-xin
    Food and Fermentation Industries. 2011, 37(03): 202-206. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.007
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    Three monoterpenes were prepared,isolated and purified from Piper nigrum L..by high-speed counter-current chromatography(HSCCC).A two-phase solvent system composed of TBME-methanol-water(2∶1∶1,v/v/v) was used.The upper phase was stationary phase and lower phase was mobile phase.By one-step elution,under the conditions of a flow rate of 2.5 mL/min and 900 r/min,35 mg of 3,7-dimethyloct-2E-en-1,6,7-triol,55 mg of p-menthane-1,2,8-triol-2-O-β-D-glucopyranoside and 40 mg of 5-hydroxy bornyl-2-O-β-D-glucopyranoside was obtained from 2.76 mg of the butanol extract of Piper nigrum L.,the purities was over 95.0% detected by high performance liquid chromatography with evaporative light scattering detection.The obtained fractions were analyzed by high performance liquid chromatography(HPLC),and identified by mass spectrometry(MS),1H-nuclear magnetic resonance(NMR) and 13C-NMR.The results indicated that HSCCC is a powerful technique for the purification of monoterpenes from Piper nigrum L.with high productive capacity and high efficiency.
  • Qian Shi-quan, Shi Ya-zhong, Wu Ya-hua, Ma Long, Zhang Bin, Zhao Da-qing, Tao Zhi-jie, Xie Hai-wei, Sun Lan-ping, Xu Hui
    Food and Fermentation Industries. 2011, 37(03): 207-209. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.012
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    In order to discuss the impact of cellulase on the extraction of resveratrol from amur grape residue,the research was investigated with the content of resveratrol as the guide and cellulose dosage,pH,temperature and enzymolysis time were determined.The enzymolysis conditions based on single factor experiment were optimized by orthogonal design.The optimum enzymolysis conditions were as follows:the cellulose dosage was 1.5 mg/g,the pH was 5.5,the temperature was 55℃ and the enzymolysis time was 1.5 h.Under these optimized conditions,the content of resveratrol was finalized at 0.680 mg/g.
  • Hu Xiao-qian, Zhu Yang-yang, Chen Le, Wu Yong-xiang
    Food and Fermentation Industries. 2011, 37(03): 210-214. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.013
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    The volatile oil extraction yield of the different parts in Gongju was compared and extraction process from fresh leaves was optimized.The volatile oil was extracted by water distillation from roots,stems,leaves,flowers and dried leaves,dried flowers of fresh Gongju.The extracting conditions from fresh leaves were optimized by orthogonal design.It showed that the extraction yield from fresh leaves was the highest,there was no significant variation in volatile oil yield from fresh leaves when pH valued at 5~9,the extraction yield was affected by particle size,soaking time,solid-liquid ratio,distillation time.The optimum extraction conditions were : particle size 4mm,soaking time 12 h,solid-liquid ratio 1∶3,the distillation time 4 h,the extraction yield 0.0020 mL/g.The optimum extraction process was stable and feasible.The results shows that the study of deep processing of Gongju leaves is necessary and it may have good application in medical and health products industries.
  • Luo Zhang, Sun Shu-guo, Fang Jun, Huang Qun, Ciren Qu-ji, Luosang Qu-pei, Dawa Ze-ren
    Food and Fermentation Industries. 2011, 37(03): 215-219. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.048
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    Myrica rubra was the material,continuous extraction method for both water soluble dietary fiber and water insoluble dietary fiber was established.The extraction technology was optimized by orthogonal test.The optimal conditions for extracting water soluble dietary fiber were:using citric acid as diffusion solvent,Myrica rubra to liquor ratio 1∶10,pH 2.0 under 90℃ for 75 min.The extraction yield reached up to 58.62%.The optimal conditions for extracting water insoluble dietary fiber were: Myrica rubra to liquor ratio 1∶12.5,pH 2.5 under 60℃ for 90min,the extraction yield was up to 61.25%.The water holding capability and hydration capability of water insoluble dietary fiber obtained in the experiment was 570.6% and 6.5 mL/g respectively.The products had better functional properties and excellent physiological activities.
  • Wang Li-wei, Zheng Yan-shan, Zhang Xiao-yu, Yu Tao, Wu Xiang-yun, Kong Tao
    Food and Fermentation Industries. 2011, 37(03): 220-224. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.049
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    The extraction of red pigment from purple Jerusalem artichoke by microwave-assisted process(MAP) was studied.Ultraviolet spectrum of red pigment in pH0.98 ethanol solution indicated that maximum absorbance was 533nm.The effect of ethanol content,ratio of material and solvent,microwave power and extraction time on extraction yield of pigment were studied through single and orthogonal design.The results showed that all factors had significant effect on extraction yield.The optimized parameters were 2% HCl-60%ethanol-water solvent,ratio of material and solvent 1∶30,microwave power 480W,50s,and extraction yield was up to 29.84 mg/g.Purple Jerusalem artichoke pigment was stable under natural sunlight,but unstable under 60℃ or heat treatment.
  • Li Ren-jie, Chen Jin-ping, Zhang Na, Yan Jing, Wang Heng-chao
    Food and Fermentation Industries. 2011, 37(03): 225-229. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.014
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    The optimum ultrasonic extraction process of proanthocyanidins was studied.Optimal extracting condition was confirmed as follows: the rate of power of ultrasonic was 100%,alcohol content was 70%,the ratio of liquid to material was 1∶12,the extraction time was 15mins and the extraction temperature was 45℃.Choose LS300 resin as the material for the purification.The absorbability of LS300 resin was studied.The results of static adsorption showed that the adsorption isotherm could be correlated with Freundlich.The adsorption equilibrium time was about 5h.The experiment of concentration gradient elution by ethanol solution showed that the most proanthocyanidins were concentrated on two parts: the 20% and 40%.The mass fractions of two parts were 86.5% and 92.3%.
  • Zhou Chun-mei, Wang Xin, Liu Bao-lin, Ren Zhi-chao, Lei Lei, Yin Xiao-mei
    Food and Fermentation Industries. 2011, 37(03): 230-234. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.050
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    The effects of different UV-C irradiation duration(10min,20min or 30min) on several quality indexes of white Hypsizygus marmoreus during passive MAP were studied,while those without UV-C treatment was taken as control.The results indicated that the shelf-life quality of postharvest white Hypsizygus marmoreus during passive MAP can be improved,e.g.,the respiration rate and the activity of PPO could be depressed,the better sensory index,rigidity,and higher total soluble sugar content can be maintained,when the UV-C irradiation duration was chosen as 20 min.
  • Ma Yan-ping, Ma Hui-ling, Liu Xing-hua, Li Shun-feng
    Food and Fermentation Industries. 2011, 37(03): 235-238. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.008
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    In order to provide the basic information and theoretical basis for storage and processing of fresh walnut,the storage physiology and nutritional quality of 'Liaohe4' dry and fresh walnut were studied,which were stored at the temperature of(0±1)℃.The results showed that,during storage,the respiration intensity and MDA content of fresh walnut were very significantly higher than that of dry walnut(P<0.01)and the POD activity was significantly higher than that of dry walnut(P<0.05),which was unfavorable to the storage and preservation of walnut.But the loss of nutrients of fresh walnut were relatively small,and especially the preservation of the amino acid and γ-VE content were high,which were very significant(P<0.01) higher than that of dry walnut.Meanwhile,the differences of content of fat,protein,VE,and the total content of δ-VE between fresh walnut and dry walnut were not significant(P>0.05).