25 April 2012, Volume 38 Issue 04
    

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    Food and Fermentation Industries
  • Wen Ming-xian~, Wang Cheng-tao~, Yang Xue-lian~, Zhao Lei~, Liu Guo-rong~, Tong Jun-mao~
    Food and Fermentation Industries. 2012, 38(04): 1-5. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.032
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    In this study,the influence of the decarboxylase(EC 4.1.1.72) gene ARO10 cloning and overexpression on the metabolic flux analysis of 3-(methylthio)-1-propanol biosynthesis in Saccharomyces cerevisiae INVSc1 was investigated.Results showed that the introduction of the recombinant plasmid pYES2-ARO10 containing ARO10 gene into S.cerevisiae INVSc1 by using the lithium acetate method resulted in the overexpression of decarboxylase.And the level of 3-(methylthio)-1-propanol biosynthesis in the recombinant strain SC10-1 was significantly enhanced.The maximum yield of 0.90 g/L was achieved when the recombinant strain was incubated for 120 h.Compared with the control strain,the yield increased by 55.2%.These results clearly indicated the decarboxylase from S.cerevisiae s288c is a key rate-limiting enzyme in the biosynthesis of 3-(methylthio)-1-propanol,and the overexpression of ARO10 gene is helpful to the 3-(methylthio)-1-propanol biosynthesis.
  • Yang Hai-rong, Zhao Gui-ming, Wang Ping, Zhao Yong-sheng, Yan Fei, Hu Yue, Chen Ying
    Food and Fermentation Industries. 2012, 38(04): 6-10. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.033
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    To provide an effective means for species identification and epidemiological tracing for Cronobacter spp.strains by studying molecular characteristic of reference strains and strains isolated from samples,38 Cronobacter spp.strains were identified by genotyping test-Genomic DNA was extracted by methods of Randomly Amplified Polymorphic DNA analysis(RAPD).One effective primer was selected with which each strain could be amplified 1 to 7 DNA fragments of 400~2 000bp.8 reference strains were identified into separate species groups.Similar to 50% for the standard,38 strains could be classified into 8 genotyping groups by genetic differences.RAPD method in this study can be applied to species identification and epidemiological tracing for Cronobacter spp.strains.
  • Chen Yuan-yuan, Chen Hao, Shi Gui-yang, Wang Zheng-xiang
    Food and Fermentation Industries. 2012, 38(04): 11-14. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.025
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    The internal transcribed spacer(ITS) region of nuclear DNA is one of the most used locus for yeast identification with growing sequence data deposited in public-access databases.This study extensively evaluated the feasibility of ITS sequence analysis for systemic classification of yeast isolates.The ITS regions of 623 yeast strains deposited in CICIM-CU were amplified by PCR and sequenced,the sequences were compared to reference data available at the GenBank database by using BLAST.Most of fungal isolates(93.3%) were assigned to species level successfully.40 strains(6.4%) were only can be assigned to genus level.2 strains cannot be identified based on ITS sequences analysis.This study shown ITS sequences were sufficiently sensitive for identifying most of yeasts,it can be powerfully and ordinarily used as first-round barcoding gene to systemic identification of bulk yeast isolates.
  • Wu Dong-mei~, He Cui-rong~, Niu Mei-can~, Peng Yu-wen~, Zheng Jia~, Jin Yang~, Huang Jun~, Zhou Rong-qing~,
    Food and Fermentation Industries. 2012, 38(04): 15-19. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.034
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    In order to investigate the diversity of microbial community in pit mud by fluorescence in situ hybridization,detection of pure culture and samples were carried out.The influenced factors on FISH were studied based on five pure culture strains including Escherichia coli,Bacillus subtilis,Lactobacillus planetarium,Acetobacter rancens,Clostridium acetobytylicum.Then the microbial community in pit mud was further investigated by the modified method.The results showed that the quantitative characterization was influenced by growth phase.The detection rate was above 82% in logarithmic and stationary phases for E.coli and L.planetarium whereas was significantly less in death phase for L.planetarium.The detected fluorescentce signal of dead cells was rather weak than live cells.For E.coli added in pit mud samples,the resolution of FISH was improved significantly through removing humic acid by aluminum sulfate,the recovery was 46.89% of the direct microscopic somatic cell count.FISH could effectively characterize the bacteria and archaea community.Detection of bacteria and archaea visualized quantitatively by FISH is beneficial to study microbial community in cellular level.
  • Xu Ze-jiang~, Meng Zhen~, Zhong Qi-ding~, Xiong Zheng-he~, Sheng Qing~
    Food and Fermentation Industries. 2012, 38(04): 20-24. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.035
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    Microbial biomass and community structure were investigated during stacking fermentation in the production of Sesame-flavor Liquor using the phospholipid fatty acid technique.The results indicated that the types and number of microorganisms PLFAs shows certain variation rules,and that can be a reflection of changes in microbial community structure.Straight chain saturated fatty acids and even-carbon unsaturated fatty acids were the predominant PLFAs.The impact of stack temperature and time on microbial biomass was obvious.Besides,the predominant microorganism is bacteria and G~-is the predominant bacteria during stacking fermentation.The difference between anaerobic and aerobic bacteria is quite visible in different samples.
  • Chen Chen~, Zhao Wei~, Yang Rui-jin~, Li Chun-yang~, Gu Yan-jie~
    Food and Fermentation Industries. 2012, 38(04): 25-28. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.037
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    The effects of pulsed electric fields(PEF) on the structures of intracellular proteins of E.coli were investigated by SDS-PAGE analysis and FTIR.The original spectrum was dissolved by Fourier self-deconvolution and curve-fitting to show the peaks of the α-helix,β-turn,random coil and β-sheet in combination with CD spectrum.The results showed PEF treatments(30 kV/cm,400μs)can induce the formation of aggregates.The contents of β-sheet decreased from 45.40% to 21.50% and the contents of β-turn decreased from 23.80% to 8.81%,while the contents of random coil increased from 0.13% to 36.95%.The contents of α-helix changes insignificantly.
  • Fan Wen-ya~, Wu Zheng-jun~, Ji Hong~, Guo Ben-heng~
    Food and Fermentation Industries. 2012, 38(04): 29-33. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.003
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    The α-glucosidase inhibitory activity of whey from fermented skim milk by 11 strains with milk curding ability which were selected from 14 Lactobacillus casei strains,assayed in vitro enrolling maltase(EC 3.2.1.20) as the targeting enzyme.It was showed that the α-glucosidase inhibitory activity of Lb.casei was strain specific,with a significant increase in inhibitory intensity with the fermentation elongated for those strains demonstrated α-glucosidase inhibitory activity.Among the tested Lb.casei strains,Lb.casei LC2W(CGMCC0828) demonstrated the strongest α-glucosidase inhibitory activity,with an inhibitory percentage of 37.36% for whey derived of 10%(w/w)skim milk fermented at 37℃ for 96 hours.These results suggested Lb.casei LC2W has the potentials of antihyperglycemia as well as anti-diabetes.
  • Zhang Gui-ying~, Zhang Cheng-ming~, Jiang Li~, Mao Zhong-gui~
    Food and Fermentation Industries. 2012, 38(04): 34-38. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.038
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    The effects on ethanol fermentation with cassava is studied,when the recycling proportion of distillery waste is 30%,50%,70% and 90% respectively.Up to eleven fermentation cycles were conducted.The results showed that,the slight influence were found in alcohol fermentation when recycling proportion is 30%,when recycling proportion is above 50%(include 50%) ethanol yield descended with the increasing of recycles.The accumulation of metabolic by-products such as organic acids,glycerin produced by Saccharomyces cerevisiae and other contamination bacteria in the distillery waste is the main reason,which Reduce the ethanol yield,The accumulation of ammonia nitrogen in the distillery waste fermentation also caused the color to deepen.
  • Lin Yan~, Zhang Wei~, Hua Xin-yi~, Li Jin-jun~, Liu Yan~, Ma Hai-yuan~
    Food and Fermentation Industries. 2012, 38(04): 39-43. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.024
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    Enzymatic hydrolysis of cellulose and straw was investigated under different hydrolysis conditions,such as reaction time,temperature and pH value.The structural change of the substrates during the process was also analyzed by scanning electron microscope(SEM).Results indicate that,the efficiency of enzymatic hydrolysis significantly varied with different pH values,and the optimal pH value was found to be in the range of 4.0~5.0.Moreover,enzymatic hydrolysis efficiency was sensitive to temperature and the optimal value of temperature was 40 ℃.Under this condition,the production rate of glucose by straw and cellulose reached 56.32% and 35.80%,respectively.Furthermore,hydrolysis process of both substrates was almost completed within 24 hours,and reached the highest efficiency after 48 hour.SEM results show that the substrate structure underwent obvious change during the first 6 hours.
  • Zhang Jie, Yang Guo-long, Bi Yan-lan, Sun Shang-de, Liu Wei, Ma Chuan-guo, Liang Shao-hua
    Food and Fermentation Industries. 2012, 38(04): 44-47. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.039
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    The porcine pancreas lipase(PPLipase) was modified by Tweens in the ethanol-water solution in this paper,the efficiency of the modification of the PPLipase was determined by incorporation of interesterification of oils catalyzed by them,and the thermal stability of the modified PPLipases and the original PPLipase was also studied.The results indicate that the modification could be finished in 30min at 40℃,however the optimum conditions for different Tweens were not the same.For Tween20,the optimum conditions were the ratio of ethanol to water 1∶2(v/v),the amount of Span20 5%.For Tween40 and Tween65,the optimum conditions were the ratio of ethanol to water 1∶2(v/v),the amount of them 1520%.For Tween65,the optimum conditions were the ratio of ethanol to water 1∶2(v/v),the amount of Tween 20%.For Tween80,the optimum conditions were the ratio of ethanol to water 1∶3(v/v),the amount of Tween80 5%.For Tween85,the optimum conditions were the ratio of ethanol to water 1∶3(v/v),the amount of Tween85 15%.The catalytic activity of the modified PPLipase was higher than that of the original PPLipase in the range of 40℃ to 80℃ and the modified lipases showed the better catalytic activity in the range of 40~70℃.The modification of PPLipase by Tweens could improve the catalytic activity and thermal stability dramatically.
  • Jiang Shou-kun, Gu Bin-tao, Xia Li-ming
    Food and Fermentation Industries. 2012, 38(04): 48-52. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.026
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    Neutral cellulase has important applications in denim biofinishing.A Pichia pastoris transformant with high activity of neutral cellulase was screened from 6 recombination transformants by fermentation experiment.The research on the neutral cellulase production by the P.pastoris recombinant was conducted under shaking flask condition,and the results were shown as follows: medium volume 30mL in 250mL Erlenmeyer flask,the initial pH 4.8 and uncontrolled during the fermentation process,inoculum volume 10% and methanol addition 1% every 24h which demonstrated an obvious enhancing effect.The above conditions allowed the activity of the neutral cellulase to reach 1144U/mL.The results from the study on enzymatic properties indicate that the appropriate catalysis conditions for the neutral cellulase from the P.pastoris transformant were pH 6.0 and temperature 50℃,and that Zn~2+ and Mg~2+ were activators of the enzyme but Fe~2+,Fe~3+ and Cu~2+ were inhibitors.The outcomes of denim biofinishing test reveal that the neutral cellulase from the P.pastoris recombinant could significantly reduce the backstaining of indigo onto denim and has a promising potential in industrial application.
  • Jiang Jun-yun, Dong Hui-jun, Yan Tong-shun
    Food and Fermentation Industries. 2012, 38(04): 53-56. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.027
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    The kinetic models of the fed-batch fermentation of cyclosporin A in 30l bioreactor were studied.Based on the fed-batch fermentation data and Logistic model,the kinetics models of cyclosporin A fermentation for cell growth,cyclosporin production and substrate consumpsion were built up.The program of origin 7.5 version was used to fit the model.The analysis results showed that the good agreement of predicted values with the experimental values,and that the kinetic models could provide reasonable descriptions for the process of cyclosporin A fed-batch fermentation.The development of fed-batch fermentation kinetics of cyclosporine A would help to regular the fermentation of cyclosporine A and increase the fermentation titer.
  • Liu Xue, Xu Yang, Li Yan-ping, Tu Zhui, Lei Da
    Food and Fermentation Industries. 2012, 38(04): 57-61. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.001
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    Formation and regeneration conditions of protoplasts for two Aspergillus strains were optimized,the effect of material,cell age,osmotic stabilizer,enzyme proportion,enzyme acting time and acting temperature on the formation and regeneration of protoplasts from Aspergillus oryzae 3.951 and Asperigullus oryzae RIB40 were studied.The optimized conditions of A.oryzae 3.951 were: mycelial as the preparation material and its age was 14 h,0.8 mol/L NaCl as the osmotic stabilizer,enzyme solution has 1.0 %(w/v) cellulase,1.0 %(w/v) lysing enzyme and 0.1 %(w/v) snailase,enzyme acting time 3 h and enzyme acting temperature 35 ℃.The optimized conditions of A.oryzae RIB40 were the same as A.oryzae 3.951 but a better age of its mycelial was 12 h.Under optimized conditions,A.oryzae 3.951 released 6.43×10~7 protoplast/g with the regeneration rate of 23.5 %,and A.oryzae RIB40 released 4.24×10~7 protoplast/g with a regeneration rate of 22.41 %.
  • Jia Chun-li~, Huang Wei-ning~, Zou Qi-bo~, Gan Xiao-hong~, Rayas-Duarte Patricia~
    Food and Fermentation Industries. 2012, 38(04): 62-66. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.040
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    The effects of frozen storage condition(0,2,4 and 6w;0,1,2 and 3c),microwave power(40% and 100%) and storage time on the moisture content,hardness,specific volume and soluble starch content of microwave-baked frozen batter-making corn cakes were investigated.Results showed that a significant increase in moisture content and specific volume and a significant reduction of hardness of the cake with the increasing of frozen storage time;freeze-thaw cycles had a more significant effect on the properties of corn cakes.Higher microwave power lowered moisture content of the cake,increased specific volume and hardness.Moisture loss and hardness increased significantly and soluble starch content decreased with storage time.Higher microwave power increased hardness and moisture loss and reduced soluble starch content at a more rapidly speed.The changes of hardness was positive related to moisture loss and soluble starch content,and the relative coefficients were 0.93 and 0.86,respectively.
  • Yan Jie~, Qiu Tai-qiu~, Yang Ri-fu~, Yang Jing-lan~
    Food and Fermentation Industries. 2012, 38(04): 67-71. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.028
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    The effects of ultrasonic intensity,ultrasonic time,temperature,usage of catalyst and reaction time on the yield of conjugated linoleic acid were studied.The mathematic model of these factors to the yield was also established through uniform design.The product was characterized by ultraviolet spectroscopy and infrared spectroscopy.The results show that the factors of ultrasonic intensity,ultrasonic time,temperature,usage of catalyst and reaction time are all important to the yield of conjugated linoleic acid.Uniform experiments indicate that comparing with other factors,the ultrasonic intensity,in the range of 22.17~27.26 W/cm~2,doesn't play an important role in the reaction.Under the conditions of the experiments,the higher the temperature,the longer the reaction time and the more usage of catalyst,will yield higher production.To obtain desirable product,the mathematic model can be used in reality to select proper parameters.In comparison with the normal method,the process assisted by ultrasound can achieve higher yield under the same conditions;it can also reduce catalyst usage and shorten reaction time with the same yield.Spectrum analysis shows that the conjugation is reacted and the cis-trans configuration is the primary products.
  • Jiang Qing-jun, Xu Xi-lin, Ren Jiao-yan, Ruan Zheng
    Food and Fermentation Industries. 2012, 38(04): 72-76. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.007
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    In order to develop aluminum-free leavening agent to replace the traditional aluminum leavening in twisted fritters,orthogonal test was used to optimize the new leavening mixed by glucose Oxidase(GOD),Pentopan,Lipase,Fungamyl and baking soda.The methods of sensory score,volume expansion and texture analysis were used to evaluate the quality of twisted fritters.The results shows that by orthogonal analysis,considering the sensory evaluation and the impact of factors in the formula,the best formula is: baking soda,GOD,Pentopan,Lipase,and Fungal α-amylase is 1.9%,80 μg/g,80 μg/g,20 μg/g,and 80 μg/g respectively.But for the hardness and chewiness of the products,the best formula is: baking soda,GOD,Pentopan,Lipase,and Fungal α-amylase is 1.8%,80 μg/g,80 μg/g,30 μg/g,and 60 μg/g respectively.Based on the integrated sensory and texture characteristics of the orthogonal test results,the optimal formula of twisted fritter is: baking soda,GOD,Pentopan,Lipase,and Fungal α-amylase is 1.8% ~ 1.9%,80 μg/g,80 μg/g,20 ~ 30 μg/g,and 60 ~ 80 μg/g respectively.
  • Li Xiang, Wen Xing, Fang Yuan, Li Jin, Wu Hao
    Food and Fermentation Industries. 2012, 38(04): 77-81. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.041
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    The sodium iron chlorophyllin by the chlorophyl form Sonchus oleraceus L was obtained through the following successive steps: saponificatioan,acidification,replacement of sodium by iron,and neutalization.Its quality was evaluated by UV-spectrophotometer.Single factor experiment and response surface method in optimization of process test conditions: Firstly,chlorophyl is saponifieated for 90min at 59℃ in the system with pH≈12;Secondly,the saponificated production is acidificated for 60min at 65℃ in the system with pH≈2~3;And afterword replaces sodium with 42mL 10%FeSO_4 for 97min.Finally,the sodium iron chlorophyllin is obtained by neutralizing at pH≈12 with 10%NaOH-ethanol.
  • Zhu Xiu-qing~, Wang Ling~
    Food and Fermentation Industries. 2012, 38(04): 82-88. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.008
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    The soybean polypeptides prepared by microbial fermentation of high-temperature soybean meal have characteristic of low production cost and high hydrolysis.Using enzyme activity as index,high-yield proteinase producing strain was screened by UV mutagenesis and genetic stability test in this study.Using hydrolysis degree as surveillance index,the strain was used to ferment extrusion and expansion of high-temperature soybean meal,and the fermentation process was optimized.The conditions of UV mutagenesis were as follows: UV watt was 20,irradiant distance was 35cm,irradiant time was 4min.Optimation of medium was performed by single factor and L_9(3~4) orthogonal experiments.The composition of optimal medium was as follows: soluble starch 2.5%,magnesium sulfate 0.04%,soybean meal 10% and Tween-80 0.5%.The cultural condition determined by single factor experiment was as follows: temperature 30℃ for 44 h,inoculum 4%,culture-age 21 h,agitation 200r/min,medium volume 30 mL/250 mL,initial pH 7.5.Using the described optimum medium and condition,hydrolysis degree of high-temperature soybean meal could reach 22.86% which was 8.34% higher than before.
  • Li Yong-xia, Qing Li-kang
    Food and Fermentation Industries. 2012, 38(04): 89-93. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.042
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    To construct the method of determining global peptidase activity on gram-positive bacillus,a L_9(3~4) orthogonal test based on single-factor experiments was designed to optimize the condition of gram-positive bacillus disruption.The content of total free amino is the main evaluation index.As results,the optimum conditions were additive volume of lysozyme 200 μL,ultrasonic power 400W,ultrasonic time 4 min,time interval 3s.11 G+ bacillus with high global peptidase were obtained from 153 strains through this method,numbered DF2-1,ZM1-1,ZM2-1,ZM3-1,DF3-1,DF3-2-1,ZMJ-5,ZMJ-2-6,ZMJ-1-1,DFM-4,WCF-2.
  • Bao Yi-hong, Liang Xue, Qin Lei, Li Rui-da, Wang Wen-qiong
    Food and Fermentation Industries. 2012, 38(04): 94-98. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.043
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    In this research we separated the exopolysaccharide from fermentation medium of Issatchenkia oriental by ethanol precipitation,and pure exopolysaccharide(EPS-I) was obtained by DEAE-cellulose52 chromatography after removing protein and dialyses.The structure of pure exopolysaccharide were determined by UV scanning.Identification of IR showed that the exopo-lysaccharide had polysaccharides specific function group such as —OH,—CH,—CO etc.but they were not proteoglycan.The hydrolyzate of pure exopolysaccharide was analyzed by Gas chromatography and the result indicated that the exopolysaccharide was mainly consisted of L-mannose,D-xylose and D-rhamnose,and their content were 3.62%,11.47% and 84.91%,respectively.Preliminary research on the antioxidant activity in vitro of EPS-I was done.Total antioxidant capacity(TAC),the scavenging rate of DPPH·,hydroxyl radical and superoxide anion were investigated in vitro.The results demonstrated that EPS-I had favorable antioxidant activity,at the concentration of 20mg/mL,the scavenging activities of DPPH· and O~-_2· radicals of EPS-I could reach to 80% above.
  • Hou Ya-kun~, Wang Sheng~, Huang Kun~, Wang Wen-jiang~, Li Di~, Wang Jian-zhong~
    Food and Fermentation Industries. 2012, 38(04): 99-103,107. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.044
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    Total enzyme addition,pH,enzymatic hydrolysis temperature and ratio of sample to solution were selected to determine the factors and levels by single-factor test method and the orthogonal test method with the hydrolysis property.The optimized enzymatic hydrolysis conditions with the Alcalase were as follows: total enzyme addition 7%(w/w),pH 10.0,enzymatic hydrolysis temperature 60℃,sample to solution 1∶25,enzymatic hydrolysis time 2h.Under these conditions antioxidative activities of hydrolysates at different hydrolysis property as high、middle、low are analyzed.The results are as follows: hydrolysates at different hydrolysis property have good ability to eliminate the DPPH,·OH,O~-_2·,and the reducing power is strong.
  • Zhao Dan~, Zeng Xin-an~, , Xu Zhong-yue~
    Food and Fermentation Industries. 2012, 38(04): 104-107. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.009
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    Grape skin procyanidins as raw material were separated according to their polymerization degrees by solvent precipitation in this paper.The capability of scavenging sodium nitrite and DPPH· were determined by spectrophotometry,and compared with vitamin C,BHT.The results demonstrated that the scavenging rate of NaNO2 and DPPH· showed the positive correlation with the concentration of the grape skin proanthocyanidins,the capability of scavenging NaNO2 and DPPH· was 94.61% and 92.56%,respectively.It was explored that with the increase of polymerization degree,the capability of scavenging decreases.
  • Liu Zhi-fang~, Wang Wen-ke~, Wang Zhi-jiang~, Liu Yu-huan~
    Food and Fermentation Industries. 2012, 38(04): 108-111. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.045
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    A comparison experiment on the hot air drying,vacuum drying and vacuum-freeze drying of Coprinus comatus was done.The content of total soluble sugar,soluble protein,Vc,rehydrated ratio and sensory quality of the dired Coprinus comatus were analyzed and evaluated.The results showed that: the content of soluble total sugar,soluble protein and Vc of dried Coprinus comatus by vacuum-freeze drying were 31.39±1.17~Aa,82.96±2.05~Aa and 0.81±0.12~Aa,the Loss significantly lower than the other two drying methods.The rehydrated ratio was 7.15±0.12~Aa,the best of the three.
  • Tu Peng, Bian Hong-xia
    Food and Fermentation Industries. 2012, 38(04): 112-115. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.010
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    Using parallel plate capacitor with different electrode size,different number lily leaves were clamped between electrode plates to determine its dielectric properties.The result shows that in different electrode size and plate spacing,loading frequency are negatively related on relative permittivity and complex impedance,and are positively related on the electric conductance.When loading frequency is about 60 K,there were extreme values of loss factor,phase angle and the quality factor,which phase angle and the quality factor were the maximum value and loss factor was minimum value.With the increase of the electrode size,the relative permittivity and conductivity values showed an increasing trend,and the impedance value was decreasing.When the quantities of lily leaf increased in the plates,the relative permittivity,loss factor and electric conductance decreased,and the phase angle,the quality factor and the complex impedance values increased.This study is expected to provide some theoretical basis and test support on the dielectric properties' nondestructive testing of Lilium davidii Var.quality.
  • Cheng Jian-guo, Dong Liang, Fu Ying-ying, Zhang Li, Guo Ji-qiang, Zhao Chang-xin
    Food and Fermentation Industries. 2012, 38(04): 116-119. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.046
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    In this paper,Saccharomyces cerevisiae cell wall proteins were taken for the application of whole cell extracted from cell wall proteins,dilute alkaline buffer extracted proteins from purified cell wall and Lysozyme and β-glucanase enzyme solution hydrolysis of purified cell wall to extracted cell wall proteins all this three extraction methods,at the same time.The differences between different algebra yeast cell wall proteins has been identified and analyzed.The results show that:the method of lysozyme and β-glucanase enzyme solution hydrolysis of purified cell wall to extract cell wall proteins can obtain more cell wall protein bands and higher purity,therefore this method is determinted the best extraction method for yeast cell wall protein extraction.Withal,by SDS-PAGE electrophoresis analysis found different generation of yeast cell wall proteins with a large difference and identified molecular weight of 36 kDa,17 kDa and 12 kDa are the three main different cell wall proteins for different generation yeasts.Among that,the expression of 36 kDa,17 kDa protein bands decreased with the age of yeast increasing,whereas,12 kDa protein band expression increased with the age of yeast.
  • Wu Bing-bing, Hao Zhen-hua, Hu Yun-shuang, Chen Xiu-fang, Jin Li-qin
    Food and Fermentation Industries. 2012, 38(04): 120-122. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.047
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    Study on the stability of Anthocyanin energy drink.According to high performance liquid chromatography(HPLC) to determine the Anthocyanin content after different processing conditions.Anthocyanin is very sensitive to temperature and light,The stability of Anthocyanin is not significant effect to K~+,Ca~2+,Mg~2+,Zn~2+and Cu~2+,which is also different effects to different sterilization way,ultra high temperature short time sterilization as to it's affect the least.Anthocyanin energy drink can be conserved at room temperature for preventing light conditions,and can be appropriately added with some metal ions to increase its nutritional value;Ultra high temperature short time sterilization can be adopted to ensures that the nutrient composition is without loss.
  • Fu Liang~, Chen Si-qian~, Yi Jiu-long~, Wu Bing-hong~
    Food and Fermentation Industries. 2012, 38(04): 123-126. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.048
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    Isolated from a Guangdong rice vinegar factory,the RF4 Gluconacetobacter xylinus is used to produce rice vinegar by surface fermentation.The effect of intact bacterial cellulose pellicle on total acidity in fermentation,the comparison of ADH enzyme activity in the pellicle and liquor,and the influence of inoculation methods on acidity,viscosity and turbidity are researched.The results show that the morphology of bacterial cellulose pellicle is very important to total acidity produced,and the ADH enzyme activity in the bacterial cellulose pellicle is 2.26×10~-2U/g,8 times of that in the liquor.The highest acidity achieved when bacterial cellulose pellicle was inoculated with Gluconacetobacter xylinus,and was taken out during the fermentation,resulting in a high yielding of 4.86g/100mL after 12 days accompanying by low viscosity and turbidity.
  • Ma Lei~, Li Hong~, Du Jin-hua~, Zhang Wu-ju~
    Food and Fermentation Industries. 2012, 38(04): 127-130. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.050
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    Green beer from a traditional primary fermentation is centrifuged to remove the yeast prior to heat treatment,then the green beer is cooled before pumping through the immobilized yeast reactor,in which wood chip is used as yeast carrier,to continuous secondary fermentation.The optimized technological parameters were gained in these experiments.In addition,beer from continuous secondary fermentation was compared with that from traditional secondary fermentation.The results were as follows: immobilization time 16 hours in room temperature;secondary fermentation temperature 10℃,time 3.1h;the reducing rate of diacetyl was largely improved.The test results indicate that there was no distinct flavor defect in beer quality after volatile flavours determination and sensory evaluation.
  • Geng Wei-tao~, Song Cun-jiang~, Xie Hui~, Feng Jun~, Xie Chen-geng~, Wang Shu-fang~
    Food and Fermentation Industries. 2012, 38(04): 131-136. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.029
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    This review summarizes the advances in the research and production of ε-poly-L-lysine(ε-PL).The biosynthetic mechanisms of the ε-PL are introduced,especially the structure of the ε-PL synthetase(Pls) and the catalytic model for ε-PL synthesis.Furthermore,the author adopts his own work to project the tendency of the ε-PL research.
  • Yu Xuan~, Li Zhao-feng~, Gu Zheng-biao~, Li Cai-ming~, Liu Wen-jing~
    Food and Fermentation Industries. 2012, 38(04): 137-140. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.051
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    Starch varies greatly in molecular and granular structures between and within botanical species,which results in the obvious differences in enzymatic hydrolysis performance.The effect of amylose,amylopectin,starch-lipid complexes,phosphate content,starch granule size and shape,granular crystalline structure,granular composite structure on enzymatic hydrolysis performance of starch were introduced.The problems and prospects of the research in this field were discussed.
  • Ran Sheng, Cui Gui-you, Wu Ting-ting, Zhu Shuang-liang, Yan Yun-qiao, Jiang Jia-jian
    Food and Fermentation Industries. 2012, 38(04): 141-146. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.012
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    With a hydroxyl group in the A-ring of daidzein C8 position,a novel isoflavone derivative which has a variety of biological activities is named 8-hydroxydaidzein.In this article,we reviewed its natural bio-source which contained exogenous and endogenous source and summarized the capable strains for biotransfomation of daidzein to 8-hydroxydaidzein.Aspergillus spp.is the big family of the capable strains.
  • Meng Liang-yu~, Huang Xiao-jie~, Xu Fang-xu~, Tang Lin~, Feng Xu-qiao~
    Food and Fermentation Industries. 2012, 38(04): 147-151. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.013
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    Wheat is one of the world's main grain crops and is also one of the eight major food allergens.Some allergens contained in wheat may have adverse effects on human health,and all of the proteins in wheat have been reported as possible allergens that exert different pathogeneses and cause various clinical symptoms.The main sensitization proteins contained in wheat include gliadins,glutenins and non-prolamins proteins.The latest research advances on physical and chemical processing properties,structural and immunological characteristics,and desensitization methods of wheat allergens are reviewed in this paper with an emphasis on its immunological aspect.
  • Dang Wan-li, Jin Li-qun, Zheng Yu-guo, Sheng Yin-chu
    Food and Fermentation Industries. 2012, 38(04): 152-158. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.030
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    Methionine is used extensively in pharmaceutical,health and fitness products,in which most of the methionine is added to feed addition.The chemical and biological technology of methionine synthesis were introduced,especially those used by the world's leading methionine production manufacturers.The main characteristics of production process were briefed;the development of methionine production was discussed.
  • Nie Qing-qing, Xu Yan, Fan Wen-lai
    Food and Fermentation Industries. 2012, 38(04): 159-164. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.022
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    γ-Lactones is a kind of important aroma compound existing in several food products(such as fruit,wine and baijiu).On the base of the important role in the aroma and flavor of food,8 γ-lactones in Chinese liquors(baijiu) were identified and quantified using the method of liquid-liquid extraction(LLE) and solid phase extraction(SPE) coupled with gas chromatography mass spectrometry(GC-MS),respectively.This method was shown to be rapid,selective and with high correlation coefficient(R~2≥0.9908,γ-dodecalactone).The detection limits of the method range from 0.22μg/L(γ-decalactone) to 4.30μg/L(γ-nonalatone),and well below the olfactory thresholds of compounds.The relative standard deviation(RSD) is less than 15%.This method can applied to γ-lactones quantification in Chinese liquors.
  • Liu Jian~, Wang Yan~, Zhang Yan Qing~, Su Hong-xia~
    Food and Fermentation Industries. 2012, 38(04): 165-170. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.052
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    In order to determine pentosan in the malt,wort and beer accurately,orcinol-hydrochloric acid and phloroglucinol methods were used to compare accuracy and recovery.The results showed that Phloroglucdinol method could determine the content of pentosan in malt accurately and several varieties of malt identified are quite different from each other.Both methods do not directly measure wort,but when fermented by yeast,orcinol-hydrochloric acid method could determinate pentosan accurately.Orcinol-hydrochloric acid method can determine pentosan in beer accurately and big differences among several varieties of beer were also determined.
  • Yu Xiao-qin~, Zhang Li-ping~, Kan Jian-quan~
    Food and Fermentation Industries. 2012, 38(04): 171-174. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.014
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    HPLC fingerprint of Pericarpium Zanthoxyli were established by fingerprinting technology.Platisil ODS C18 Column(250 mm×4.6 mm,5 μm)was used with acetonitrile-water(50∶50) as mobile phase at a flow rate of 0.8 mL/min.The detection wavelength was at 254 nm.The column temperature was at 22 ℃.The characteristic of Pericarpium Zanthoxyli fingerprint was established by《Evaluation Systems of similarity of the chromatographic fingerprint of traditional Chinese medicine,version A》(researchful version).Fingerprint of Zanthoxylum Bungeanum Maxim has eleven common peaks,fingerprint of Zanthoxylum Schinifolium Sieb.et Zucc has ten common peaks.Referring to common peaks,similarity value of Zanthoxylum Bungeanum Maxim and Zanthoxylum Schinifolium Sieb.et Zucc was calculated.The results showed fingerprint similarity of Zanthoxylum Bungeanum Maxim was high(amount similarity>0.97),but fingerprint of Zanthoxylum Schinifolium Sieb.et Zucc was low similarity(0.52~0.67).Quality of Zanthoxylum Bungeanum Maxim from different origins was more consistent,while Zanthoxylum Schinifolium Sieb.et Zucc has big difference.Characteristic difference between peak of HPLC fingerprint of Zanthoxylum Bungeanum Maxim and Zanthoxylum.Schinifolium Sieb.et Zucc was identified by TOF/MS,and was inferred to hydroxyl-γ-sanshool or 2'-hydroxyl-N-isobutyl-2,4,8,10,12-tetradepentaenoateamide or their structural isomer.
  • Duan Bin-wu, Xu Xia, Sun Cheng-xiao, Fan Chang-yu, Guo Wang-mo, Zhu Zhi-wei
    Food and Fermentation Industries. 2012, 38(04): 175-179. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.015
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    A spectrophotometry method for determination of sulfite contents in edible fungus was successfully established and compared with the published National Standard Method(NSM).The method involves nitrogen distillation followed by formaldehyde absorbing and parasosaniline colorimetry.The effects of distillation duration,nitrogen presence and flow rate on testing results were also investigated.The linear range of the calibration curve for sulfite was 0-20 μg.The sulfite detection limit was 0.08 mg/kg with 2.0 g of samples.The relative standard deviation of the proposed method ranged from 1.2 % to 7.8 %.The recovery rate was between 83.1% ~ 99.2%.The most suitable distillation duration was 25 min.Use of nitrogen could improve detection sensitivity by 9-19 %,and the optimal nitrogen flow rate was 0.6-0.6 L/min.This newly established method was validated by 5 licensed laboratories for accuracy.The reported new method has advantages of higher sensitivity,less interference from sample colors and environmental hazards when compared to the NSM.
  • Chen Yue-jiao, Chen Hai-guang, Bai Wei-dong, Ouyang Chu-jie
    Food and Fermentation Industries. 2012, 38(04): 180-183. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.031
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    In order to explore the distinguishing indexes of the Camellia oil between the purity and the adulteration,the sensory and fatty acid composition of the Camellia oil were studied in this paper.At the same time,the same indexes of peanut oil,rapeseed oil,corn oil,sunflower oil which the Camellia oil which maybe adulterated with were analyzed and compared.Then the Camellia oil was mixed with four kinds of oil by different ratio,and characteristics changes were studied.The sensory evaluation results indicated that the purity of Camellia oil appeared as light yellow with a little green,with a little tea aroma.It was not sticky.Analyzing by GC-MS,the results indicated that the main component of fatty acids was oleic acid,its composition was upon to 80%,and a small amount of hexadecanoic acid,linoleic acid,stearic acid and trace 11-eicosenoic acid.The characteristic indexes changed with different proportion of four kinds of oil mixed.The results could be applied to identify the adulteration of Camellia oil from the market.
  • Chen Mei-feng
    Food and Fermentation Industries. 2012, 38(04): 184-187. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.023
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    A silver doped polyglycine modified electrode was prepared to yield thin and stable polymeric films by cyclic voltammetry(CV).The electrochemical behavior of vitamin C at the modified electrode was studied.A novel method for determination of vitamin C using cyclic voltammetry was established.The modified electrode showed an excellent electrocatalytical effect on the oxidation of vitamin C.In pH5.0 phosphate buffer solution(PBS),the oxidation peak current versus the concentration of vitamin C showed a linear relations over the range of 1.6×10~-6~3.0×10~-3 mol/L with a correlation coefficient as follows:i_pa=-8.48×10~-7+0.023c(mol/L),R=0.999 9;the detection limit was 1.2×10~-7 mol/L.The recovery was 95.0%~101.0%.Major interferences such as vitamin A,citric acid and malic acid were tested at this silver and polyglycine compound modified electrode and found that selective detection of can be achieved.This new method has been successfully applied for the determination of vitamin C in cayenne with satisfactory results in this test.
  • Long Yue~, Huang Xian-zhi~, Ding Xiao-wen~, Luo Jin-feng~, Ren Mei-yan~, Wang Jian~
    Food and Fermentation Industries. 2012, 38(04): 188-191. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.016
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    To analyze of fatty acid compositions in eri silkworm pupa oil and to provide scientific basics in the comprehensive utilization of eri silkworm pupa.Method: Crude lipids were extracted by organic solvent,then gas chromatography-mass spectrogrophy(GC-MS) and gas chromatography(GC) were used in the analysis of fatty acid.Result: The lipid of eri silkworm pupa is relative less(19.16%).The fatty acid compositions are C18∶3(43.41%),C16∶0(21.15%),C18∶1(11.59%),C18∶2(10.28%),C18∶0(8.08%),C16∶1(1.88%),C14∶0(0.93%),C15∶0(0.31%),C12∶0(0.16%)and cyclopropaneoctanonic acid(0.16%).The most abundant fatty acid is C18∶3(57.63%),and C16∶0(20.62%),C18∶1(7.68%),C18∶2(6.28%) and C18∶0(3.38%) are less.
  • Wang Gui-ying~, Yin Hong~, Cao Jin-xuan~, Shi Zhong-fen~, Liao Guo-zhou~
    Food and Fermentation Industries. 2012, 38(04): 192-195. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.053
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    Experimental Xuanwei hams were processed according to traditional processing technology with 40 green hams weighted 9~11kg.Approximately 100 g of muscle from the Biceps femoris and the around the muscles from 5 hams each time was obtained on 24 h,18 d,125 d,250 d and 360 d and then minced.Then biogenic amines content were determined with HPLC.The results showed that tryptamine,phenylethylamine,putrescine,tyramine,cadaverine,spermidine and spermine were detected in Xuanwei ham.The content of spermine was the highest,followed by that of tyramine and spermidine during the processing period.There were no significant difference in the content of tyramine,spermine and spermine among the various processing stages(P>0.05).The content of phenylethylamine and putrescine were increasing during the processing(P<0.05),at the mid-ripening(250d) stage,the content of phenylethylamine and putrescine were up to the highest level,and then decreasing(P>0.05).The total content of biogenic amines was increasing during the processing period.In the mid-ripening(250d) stage,the content of biogenic amines was up to 11.51 mg/100g.After the mid-ripening stage,the total content of biogenic amines started to decrease(P>0.05).
  • Guo Rui, Peng Yi-kai, Zhou Guang-hong, Xu Xing-lian, Jiang Bing, Wu Ju-qing
    Food and Fermentation Industries. 2012, 38(04): 196-201. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.054
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    Freeze-thaw Cycle can destroy pork's quality.This study is designed to investigate effects of kappa-carrageenan and compound-phosphates on pork sausages' quality.The sausages were made of frozen-and-thawed pork meat.The results indicate that for limited frozen-and-thawed pork,kappa-carrageenan can improve edible quality of sausages,increase the yield and enhance the TPA;compound-phosphates can improve the water holding capacity and water distribution in the sausages.
  • Wu Wen-fei, Yu Xiao-lin, Hu Zhou-yan
    Food and Fermentation Industries. 2012, 38(04): 202-207. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.055
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    Effects of nisin and lysozyme on improving the quality of fresh-cut litchi during ice-temperature storage were evaluated.Results showed that comparing to the control,both nisin(0.05%) and lysozyme(0.05%) treatment could decrease colony count and total combined molds and yeasts count of fresh-cut litchi significantly.Nisin exhibited higher inhibitory effect on bacteria and lower inhibitory effect on molds and yeasts compared with lysozyme,meanwhile lysozyme could protect the color of fresh-cut litchi.The mixture of biological preservatives made better effect for keeping quality of fresh-cut litchi during ice-temperature storage than single biological preservative.The fresh-cut litchi treated by biological preservative mixture asnisin 0.025% and lysozyme 0.025% showed less microbe quantity and better color.
  • Chen Yi-zhao~, Wang Yi-jia~, Gang Cheng-cheng~, Li Jian-long~, Gao Jian-gang~, Qian Wei-dong~, Wang Ke-feng~, Yu Zhong~
    Food and Fermentation Industries. 2012, 38(04): 208-211. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.017
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    The "baihua" peaches were treated with 1%,2% chitosan oligosaccharide(COS) and 1% chitosan(CTS) respectively at(3±1)℃.The results shows that 1% CTS coating was the most effective to reduce respiratory rate of fruits.No significant respiration peak was detected during the storage and it was lower compared with others method after 10 days of preservation.COS with a 2% coating effectively reduced the decrease of firmness and TSS.This treatment also had good effects on maintaining firmness,inhibiting the increase of relative conductivity,MDA and PPO.The result also indicated that the higher concentration of COS coating,the better effect on the perseveration.However,the best dosage of COS for preservation of peach fruit still needs further research.
  • Wang Kai-tuo~, Zheng Yong-hua~, Shang Hai-tao~, Zhang Liang~, Tang Wen-cai~
    Food and Fermentation Industries. 2012, 38(04): 212-218. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.018
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    The present study was to investigate the effect of the MeJA treatment on grapes fallen from the stems during the storage at 1 ℃ and its mechanism.The results showed that the 10 μmol·L~-1 MeJA treatment could significantly reduced the fallen rate and decay incidence during the storage,and delayed the increase of ABA content,ethylene release and ABA/GA ratio,and inhibited the decrease in PME activity and increased in PG activity.Meanwhile,the MeJA-treated bunch exhibited significantly higher AIR and Na_2CO_3-soluble pectin contents compared with the controls.The water-and CDTA-soluble pectin contents in the MeJA-treated zone were lower than the controls.Therefore,these results suggested that MeJA treatment significant reduced the fallen rate in harvested grapes and improved fruit quality.The mechanism may be by the regulation of endogenous hormone level and the balance of the activities of cell wall hydrolases PME and PG,which inhibited the hydrolysis in cell wall and maintained the complete cell wall structure.
  • Xie Shu-hui~, Wang Wei-qin~, Ye Wei-dong~, Huang Shao-bo~, Shao Xing-feng~
    Food and Fermentation Industries. 2012, 38(04): 219-223. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.020
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    The natural rot Shatang orange fruits were used to isolate and identify the pathogen.,The effects of nano TiO2-chitosan coating on the quality of Shatang orange and inhibition of fungistatic ability in vitro experiment were investigated.The results showed that the main pathogenic fungi on Shatang orange was Penicillium digitatum.The coating contained with 2% chitosan and 2% nano TiO2 can maintain higher firmness,soluble solids and titratable acid and Vc content,significantly reduce the growth of P.digitatum and the lesion diameter.It was suggested that the nano TiO2-chitosan coating can maintain the natural quality and inhibit the postharvest diseases of Shatang orange.
  • Pang Ling-yun, Zhan Li-juan, Li Yu, Zhu Mei-yun, Chen Yao-hua
    Food and Fermentation Industries. 2012, 38(04): 224-227. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.04.021
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    Cherry tomatoes were stored under different levels of pressure(101.3 kPa,80 kPa,50 kPa,20 kPa).A series of parameters of cherry tomatoes were measured at intervals of 4d to evaluate the preservation effect by hypobaric treatment.Results showed that hypobaric treatment could reduce the weight loss rate,respiratory intensity of cherry tomatoes,maintain Vc content,titration acidity,soluble solid content and protopectin content,restrain the rising of cytoplasm membrane permeability and anthocyanin content in some extent,and hold the better storage quality.Cherry tomatoes treated with 50 kPa preserved the best quality during storage.