ZHAO Qi,ZANG Xiao-yun,HU Hai-feng
Food and Fermentation Industries. 2015, 41(1): 234.
To establish an analytical method of simultaneous determining of neochlorogenic acid,chlorogenicacid,isochlorogenic acid A,isochlorogenic acid C-four bioactive compounds from Mate Tea(llex paraguariensis) usingpCEC (pressurized capillary electrochromatogaphy).The mobile phase was composed of methanol and acetonitrilecontaining phosphoricacid (pH 2.5,2 mmol/L) with the flow rate of 0.07 mL/min.Chromatography was monitored at wavelength 325 nm and the separation voltage was-10 kV.The contents of four phenolic components in 3 batches of samples collected from different typeswere determined and compared.The correlation coefficients between concentrations and chromatographic peak areas of neochlorogenic acid,chlorogenic acid,isochlorogenic acid A,isochlorogenic acid C wereover 0.999 and the linage ranges were 0.072 ~ 1.2,0.048 ~ 0.8,0.072 ~ 1.2,0.17 ~ 2.8mg/mL,respectively.The precisionwas 1.10%,2.12%,2.98% and 2.32%,respectively.The repeatabilitywas 1.10%,2.12%,2.98%,2.20%,respectively.The average recoveries of the four compounds were 98.86%,99.03%,99.48% and 99.21%,respectively.Themethod is sensitive,accurate,and can be used for the analysis of phenolic compounds from Mate tea(Ilex paraguariensis).Compared with HPLC,organic reagents used in the experiment,the dosage were much lower than normal dosage of HPLC detection.The method has great feasibility in the quality control of polyphenols and their mass composition detection.