25 August 2015, Volume 41 Issue 8
    

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  • REN Duan-ping,XI Wen-jing,REN Bo
    Food and Fermentation Industries. 2015, 41(8): 1.
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    The new version of the Food Safety Law was reviewed and approved by the Fourteenth Session of the Standing Committee of the Twelfth National People's Congress on April 24,2014.The new law has 154 provisions totally,with 50 provisions being added up and 70 percent of the content modified substantially.Thenew Food Safety Law,on the basis of summarizing national experiences and learning from international good practices,increases the fundamental principles of food safety,consolidates and deepens the responsibilities of the food safety supervision and management organ,reforms and innovates the food safety management system,strengthens the source treatment of food safety,and regulates the obligations that should be undertaken by food manufacturers and operators,local people' s governments and the governmental supervision and management organs respectively.By perfecting the social multigovernance,embodying the law idea of combining punishment with leniency and responding to the wishes and value appeals of the mass in a concentrated way,the Food Safety Law witnesses and implements a series of key policies and decisions of the State Council as well as the CPC Central Committee concerning the food safety issue.
  • LI Zi-jie,HE Bei-bei,GAO Xiao-dong
    Food and Fermentation Industries. 2015, 41(8): 7.
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    fda gene encoding FruAS.car from Staphylococcus carnosus and yqaB gene encoding YqaB phosphatase from Escherichia coli were inserted into the expression vector CDFDuet-1 to obtain the recombinant plasmid CDF-fda-yqaB.The plasmid CDF-fda-yqaB was transformed into E.coli BL21Star (DE3).The engineered E.coli BL21Star (DE3)/CDFDuet-1-fda-yqaB with capacity of co-expression of aldolase FruAS.car and phosphatase YqaB was used as the fermentation strain.The rare ketoses were synthesized with dihydroxyacetone phosphate (DHAP) generated in cells via glycolytic pathway as the the donor molecular and the propionaldehyde (butyraldehyde) as the acceptors.The ketose products were analyzed by HPLC and confirmed with 1H NMR after purification.In addition,the isotope experiment was performed using [U-13C6] labeled glucose as the sole carbon source with propionaldehyde as the acceptor.The result showed that C1,2,3 of the product derived from DHAP were ultimately from glucose.
  • LIU Li,WANG Fa-song,LI Yan-jun,XIE Xi-xian,CHEN Ning
    Food and Fermentation Industries. 2015, 41(8): 12.
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    Bacillus amyloliquefaciens TA208-LM is a guanosine producer with adenine auxotrophy.The adenine concentration in the fermentation broth is a critical factor because too little adenine will affect the growth of bacteria,while too much will restrain the synthesis of keyenzymes.Firstly the type of adenine donor and its appropriate concentration were optimized.The results indicated that Springer 2506 was the best adenine donor,and 15 g/L was optimal for ribavirin accumulation.However,the concentration of adenine released from this dose of Springer 2506 was too high at the end of fermentation.Therefore,the initial quantity of Springer 2506 was reduced and extra adenine was added.The results indicated that the activity of PRPP amidotransferase and the titer of ribavirin were both highest when the optimal initial adenine concentration was 47.95 mg/L and accordingly the adenine in the fermentation liquid was maintained at 40 ~ 50 mg/L.This data indicated that controlling the initial addition of yeast extract and further feeding appropriate quantity of yeast extract was likely a feasible way to control adenine concentration in the fermentation.Springer 2506 fed-batch fermentation in 7.5 L fermentation tank was studied.When 7.5 g/L Springer 2506 was used as substrate and another 7.5 g/L Springer 2506 was fed into the fermentation broth,the ribavirin production increased by 11.29% compared to one-time addition of 15 g/L Springer 2506.The study on limited control of adenine would provide some useful guidance for industrial production of ribavirin.
  • WANG Tao,HU Xian-qiang,YOU Ling,WANG Song,YU Yu-you,JIAN Xiao-pin
    Food and Fermentation Industries. 2015, 41(8): 18.
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    20 yeast strains isolated from strong-flavored liquor at several stages of the fermentation were inoculated in special medium containing fermentative grain and yellow water and fermented for 50 days in dark.Then the contents of acid,alcohol and ester in fermented grain were detected.The fermenting grain without yeast inoculation was taken as the blank control and the fermenting grain in the pit was used as the normal control.The result showed that the contents of acetic acid,butyric acid,ethanol and ethyl lactate in all fermentation systems outside the pit (including the blank) were higher than those in the normal control.The contents of ethyl caproate,ethyl acetate and ethyl butyrate were lower than those in the normal control.Significant linear positive correlations were observed between acetic acid and ethyl acetate,and between butyric acid and ethyl butyrate,while irregular relationship existed between caproic acid and ethyl caproate.However,promotion of ethanol production (up to 9.70 mL/100 g) with 16 yeast strains and increase of esters content (up to 11.52 g/L) with 12 strains in fermentative grain were observed when compared with the blank control.Especially the strain Z8Y-15 which isolated from the air of workshop and identified as Pichia sp.showed positive prospect in promoting the forming of ethanol and ethyl caproate in fermentative grain.
  • XIONG Tao,LIAO Liang-kun,HUANG Tao,DENG Yao-jun
    Food and Fermentation Industries. 2015, 41(8): 23.
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    To investigate the application of spray drying on L.plantarum NCU116 starter culture,it was found that the best formulation of protective agent was 5% (w/w) skim milk,5% (w/w) trehalose and 3% (w/w) monosodium glutamate through orthogonal experiment.Meanwhile,the response surface methodology was used to optimize the spray drying process.The recommended optimum spray drying conditions were inlet temperature of 125 ℃,material flow of 320 mL/h and microbial contents of,10.25 lg CFU/mL.Under this condition,the survival rate of NCU116 was 89.95 % and the viable counts were 10.96 lg CFU/g.The impact of protective agent on the membrane integrity was investigated using flow cytometry.Cells spray drying with protective agent significantly reduced membrane damage and 49.75 % cells were intact after addition of protective agent,which was only 1.12 % before it.All microcapsules showed a spherical shape with concavities and free of fissures and particle sizes between 10 and 18 μm through scanning electron microscope.The glass translation temperature of this starter culture was 79.50 ℃ as determined by differential scanning calorimetry.The storage stability of dried powder showed significant difference at different storage temperature.Storage of starter culture at-20 ℃ led to slightly viability loss after 60 d,while storage at 25 ℃ for 60 d led to 2.31 Log loss of viable cells.The outcome of this study provided a basis for production of starter culture using spray drying.
  • ZHAO Zhong-sheng,ZHU De-qiang,WANG Yong-yuan,ZHENG Zhi-yong,ZHAN Xiao-bei
    Food and Fermentation Industries. 2015, 41(8): 30.
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    To investigate the effect of exoR gene on the cell growth and curdlan production of A.grobacterium sp.ATCC 31749,a suicide plasmid pJQ-exoR-kan containing two homologous sequences of exoR gene was constructed and transformed it into wild type strain by triparental conjugation method.An exoR gene knockout strain was obtained.Compared with the wild type strain,the cell growth of mutant strain was inhibited.Meanwhile,the curdlan production level and the substrate conversion rate were increased by 16.8% and 19%,respectively.The results indicated that exoR was involved and played a positive role in curdlan biosynthesis,which further improved the study of the metabolic pathway and regulatory network of curdlan in Agrobacterium sp.ATCC 31749.
  • ZHANG Fu-sheng,HU Guo-zhou,TIAN Mei-ling,LI Man-shan,KAN Jian-quan
    Food and Fermentation Industries. 2015, 41(8): 36.
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    The purpose of this work was to explore an efficient application of microwave treatment in enzymatic modification.The effects of microwave treatment on the enzymatic activity,property and kinetic parameters of immobilized glucose isomerase (GI) were investigated.Enzyme activity was 156.13U/g when 400 W and 70 ℃ were applied,which was increased by 24.50% compared to control,indicating that microwave had an positive effect on enhancing activity of immobilized GI.Moreover,microwave treatment increased the Vmax while decreased the Km,and had barely effect on the optimum pH and ion concentration of immobilized GI.In addition,microwave treatment slightly decreased the optimum temperature and high temperature stability of immobilized GI,but these parameters were still good enough to meet requirements of practical application.
  • YU Chun,YU Ji-cheng,ZHANG Chun-hong,LIU Qiu,CHEN Jiao
    Food and Fermentation Industries. 2015, 41(8): 42.
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    Exo-inulinase was purified from the fermentation broth of Streptomyces griseoplanus S501 using the ammonium sulfate fractionation combined with Sephadex G-75 gel filtration chromatography and DEAE-sepharose CL-6B ion-exchange.The purification index of this enzyme was 10.42 times and its specific activity was 249.5 U/mg.The molecular weight of the purified exo-inulinase identified by SDS-PAGE was about 28.184 kDa.The optimal temperature and pH for its reaction were 50 ℃ and 5.0,respectively.Under this condition,its Km and vmax for soluble inulin were 3.83 mg/mL and 4.64 mg/(mL · min).The effects of different metal ions on enzyme activity showed that the enzyme activity was strongly activated by Cu2+,Ca2+ and Ag +.However,Mg2+,I-,Li +,Fe3+,Al3+ and K + inhibited the enzyme activity at various extents.
  • GAO Lei,XIE Jing,YE Zao,ZHANG Ning,WANG Yi-lin,GAO Chen-yue-yang
    Food and Fermentation Industries. 2015, 41(8): 48.
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    In order to control microbial contaminant of chilled chicken in targeted way,dominant spoilage bacteria isolated from deteriorated chilled chicken leg were screened using selective medium and sensory evaluation.Using the structure of phylogenetic tree,they were identified by traditional physio-biochemical tests combining with 16S rDNA sequence analysis.The sterilized chicken legs were stored at 4 ℃ after inoculated with each identified strain.The bacterial population and total volatile basic nitrogen (TVB-N) value in the 1 st,2nd,4th,6th and 8th day were determined respectively.YTVB-N,the yield factor of microbial metabolites,was selected to evaluate the spoilage capability of strains.The results showed that five strains of dominant spoilage bacteria,designated as J3,S1,M3,V1 and J1,were obtained and identified as Pseudomonas pseudoalcaligenes,Brochothrix thermosphacta,Staphylococcus saprophyticus,Achromobacter xylosoxidans,and Serratia grimesii,respectively.By calculating YTVB-N,it could be found that spoilage capability of J3 was the strongest among the spoilage bacteria under 4 ℃ cold storage,and V1 was the weakest,however.
  • WAN Hui-da,LI Dan,ZHANG Jue
    Food and Fermentation Industries. 2015, 41(8): 54.
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    Biotransformation of ginsenoside Rg3 using a β-galactosidase from Aspergillus sp.was evaluated preliminarily.To improve the aqueous solubility of Rg3,an inclusion complex with hydroxypropyl-β-cyclodextrin was prepared before enzymatic hydrolysis.The solubility of Rg3 increased 74.6 folds and the phase solubility curve displayed a typical AL type indicating the formation of 1 ∶ 1 inclusion complex.The β-galactosidase from Aspergillus sp.presented specific hydrolytic activity on β-1,2 glucosidic linkage at C3 of ginsenoside Rg3,and converted Rg3 to Rh2.Using an enzyme loading of 500 U/g Rg3,the highest conversion of 90.6% and yield of 88.5% were obtained after reaction at at 60 ℃ for 24 h.
  • WEI Yu-jie,FU Fang-yuan,WU Shun,ZOU Wan,XUE Jie,YAN Yin-zhuo,WU Yun
    Food and Fermentation Industries. 2015, 41(8): 59.
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    Urea produced by arginine metabolites from Saccharomyces cerevisiae has very important effect on the content of ethyl carbamate in wine.In this paper,the fermentation performance of 18 S.cerevisiae strains and the correlation between the expression level of car1 gene and the content of EC and arginine were analyzed.Real-time fluorescence PCR showed that the expression of car1 gene increased at the beginning of fermentation and decreased two days after fermentation.The expression level of car1 gene was significantly negatively related with the content of arginine with the correlation coefficient between 0.990 3 ~ 0.997 7,while it was positively related with the content of carbamate ethyl with the correlation coefficient between 0.624 9 ~ 0.995 8,which was obviously different between different yeasts.Selection of S.cerevisiae strains containing arginase with low activity could effectively reduce the content of Ethyl carbamate in wine.
  • SHAO Shu-ping,ZHANG Cun-sheng,WANG Zhen-bin,REN Xiao-feng,CUI Feng-jie,ZHAO Peng-xiang,ZHANG Jun-hong
    Food and Fermentation Industries. 2015, 41(8): 65.
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    Effects of nitrogen sources addition on mesophilic anaerobic fermentation of vinegar residue were investigated in batch tests.Urea,NH4Cl and KNO3 were used as organic,ammonium and nitrate nitrogen respectively.Under the optimum C/N ratio,the variation of hydrogen yield,fermentation style and nitrogen form were studied.Results indicated that the optimum C/N ratio was 40 for vinegar residue fermentation.The hydrogen yield increased from 54 mL/g-VS to 65,85 and 78 mL/g-VS by addition of urea,NH4Cl and KNO3,corresponding to 46%,57% and 21%,respectively.The fermentation type was changed from butyrate-type to ethanol-type as a result of NH4Cl and urea addition.Ammonium was the main form of nitrogen in the fermentation liquid.The total nitrogen increased at the end of fermentation,while the nitrate nitrogen decreased significantly.
  • DU Wei,PIAO Yong-zhe,ZHOU Yan-pin,WANG Xiao-yu,ZHAO Chang-xin
    Food and Fermentation Industries. 2015, 41(8): 70.
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    In order to expound the secretion protein changes during yeast aging,the yeast was cultivated in saline and its extracellular protein content and activities of antioxidant enzymes and protease were determined.The effect of long term culture on the senescence of these cells was evaluated.Total extracellular proteins were extracted and separated by two-dimensional electrophoresis,and compared with extracellular protein of normal (YNB) culture.Then the different points between them were identified by MALDI-TOF-MS.Under the condition of continuous aging the intracellular antioxidant enzyme activity was reduced,while the activity of protease and the amount extracellular protein increased at the same time.The results from MALDI-TOF-MS showed that extracellular protein contains some intracellular protein fragments.It might be related to the increasing activities of protease in cells.Intracellular " useless proteins" were hydrolyzed into pieces to extracellular environment.
  • LIU Bo,YUAN Li-peng,LIN Dan-qiong,XIONG Bo,GAO Xiu-jie
    Food and Fermentation Industries. 2015, 41(8): 75.
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    Preparation and identification of polyclonal antibody against Isocarbophos (ICP) was studied.The hapten H-ICP was designed and synthesized on characteristics of ICP pesticide,and artificial antigens H-ICP-BSA (immmue antigen) and H-ICP-OVA (coating antigen) were prepared by coupling with the carrier proteins using active ester method.The polyclonal antibody (PAb) against ICP pesticide was produced by animal immunity.Indirect ELISA experiments showed that IC50 was 5.3 ng/mL,linearity range was from 1.77 to 10.06 ng/mL.There is no cross reaction against other structure analogous.The cloning of PAb against ICP was successfully obtained which could be used in pesticide residue detection in fruits and vegetables.
  • HE Nian-wu,LI Dan
    Food and Fermentation Industries. 2015, 41(8): 79.
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    In order to study the Shangluo green tea polysaccharide (SGTPs) in vitro antioxidant and antitumor activities,DEAE-52 fiber column chromatography is adopted to purify tea polysaccharides water extracts.Then,based on vitro antioxidant system,its scavenging ability to DPPH free radical,ABTS radical,hydroxyl radical,superoxide anion was tested.Meanwhile.Its inhibition effect on the growth of MCF-7 breast cancer cell was tested.The results showed that,the content of polysaccharide in SGTPs-1,SGTPs-2,SGTPs-3 obtained by DEAE-52 cellulose column chromatography were 83.5%,76.8% and 89.7% respectively.The above three components showed good concentration-dependent of scavenging ability to different free radical.Among them,the strongest component was SGTPs-3,followed by SGTPs-1,finally SGTPs-2;the three components of SGTPs have certain inhibition ability to the growth of MCF-7,and the inhibition rate is positively correlated with concentration.Thus,polysaccharide in Shangluo green tea have the antioxidant and the antitumor activities in vitro.
  • DUAN Wei,WANG Li,QIAN Hai-feng,ZHANG Hui,QI Xi-guang
    Food and Fermentation Industries. 2015, 41(8): 84.
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    The producing process of brown rice pasta was optimized.The basic materials were brown japonica rice and brown indica rice with a ratio of 1∶1 and 8% soy protein isolate.Double screw extruder was used in making pasta.The best formula for raw material is:brown japonica rice flour 50 g,brown indica rice flour 50 g,soy protein isolate 8 g,guar gum 0.4%,sodium alginate 0.4%,xanthan gum 0.4%,monoglyceride 0.8%.And the best extrusion condition is:moisture 28% (The initial moisture content of raw materials is adjusted to 12%),screw speed 120 r/min,and temperature 120 ℃.The brown rice pasta has the similar properties with commercial pasta.
  • LIN Xiao-jie,WENG Bo-qi,LIN Xiao-zi
    Food and Fermentation Industries. 2015, 41(8): 90.
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    Effects of different drying methods including 40℃,60 ℃ and 80 ℃ hot air-drying and freeze-drying on the content of nutrient and physical properties of Cordyceps militaris fruiting bodies were studied.In conclusion,low temperature drying method is good for SOD enzyme activity,SOD enzyme activity in C.milita by lyophilization was 36.73U/mg;high temperature (80℃) or long time (24h) drying process were not favorite to preserve cordycepin.Cordycepin content was 148.84 μg/g by 60℃ drying and 143.14μg/g by freeze-drying for 24 hours.High temperature and short time was better for higher adenosine content.Adenosine content was 592.01μg/g at 80℃ drying for 6 hours.Four drying methods had no obvious effect on swelling properties of super micro-milling powder.Lyophilization method had the lowest flow ability.
  • XU Yao,CHEN Xue-feng,LIU ning
    Food and Fermentation Industries. 2015, 41(8): 93.
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    The apple soluble dietary fiber was sulfated by using ultrasound-assisted amino acid-N,N-dimethylformamide.The degree of substitution was chosen as the indicator for the single-factor experiment and the orthogonal experiment to optimize sulfation conditions.The results showed that the optimal technology parameters were as follows:the mass ratio of soluble dietary fiber and amino acid (g∶ g) 1 ∶ 3,reaction temperature of 60 ℃,ultrasonic frequency 35 kHz,and the volume ratio of soluble dietary fiber and DMF (g∶ mL) 1 ∶ 70,reaction time 1.5h.Under the optimal conditions,the degree of SSDF reached to 0.97.From FTIR,the apple soluble dietary fiber after sulfated had the typical sulfated functional groups.
  • WANG Qiang-wei,SHI Xian-zhen,Md Ramim Tanver Rahman,WANG Hong-xin,SU Yi-hai
    Food and Fermentation Industries. 2015, 41(8): 97.
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    The content of zingerone,6-gingerol,8-gingerol,6-shogaol,10-gingerol in fresh ginger and dried ginger made by different drying methods were compared by HPLC.The HPLC separation was carried out with a Grace smart RP C 18 column (4.6mm × 250mm × 5 μm) using the mobile phases composed of acetonitrile (B) and 0.1% aqueous acetic acid (A) in gradient elution mode at a flow rate of 1.0 mL/min.The detection wavelength was 280 nm and the column temperature was set at 30℃.The total content of zingerone,6-gingerol,8-gingerol,6-shogaol,10-gingerol in dried ginger made by vacuum freeze drying was the highest with a large amount of energy within 48h.The content of gingerols in dried ginger made from infrared heating at 60 ℃ was the second one with a very high drying efficiency of 1.5h.Different drying methods have significant influence on the content the gingerols.The most proper infrared heating should be at 60℃.
  • WU Na,LIU Ling,ZHOU Ming,SUN Hui,LI Guo-ming,JIANG Zhong-jie
    Food and Fermentation Industries. 2015, 41(8): 101.
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    In this paper,we focused on the preliminary characters of recycling potato protein through membrane technology.We analyzed the molecular weight distribution,amino acid content,functional properties,and alkaloid content of potato protein through SDS-PAGE and HPLC.The results suggested that the potato retained patatin and protease inhibitors,which are the two major proteins of potatoes.Its nutritional value and functional properties were equivalent to soybean protein isolate;it had lower alkaloid content compared to the result of the thermal coagulation method.
  • SHI Hong-ling,JIAO Zhu-jin,TANG Cun-duo,WANG Rui,YAO Lun-guang,KAN Yun-chao,WU Min-chen
    Food and Fermentation Industries. 2015, 41(8): 105.
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    In order to explore the optimal process of producing mannooligosaccharide by complex enzyme,konjac powder was hydrolyzed by recombinant β-mannase (reAuMan5AN3C3) with high thermostability and different units of the recombinant β-endoglucanase (reAuCel12A) with high activity in this study based on synergistic hydrolysis of konjac powder with β-mannase and β-endoglucanase.Through the research on the factors of proportion of the two enzymes,hydrolysis pH value,hydrolysis time,hydrolysis temperature and substrate concentration,the optimum proportion and reaction conditions were determined.Also,the effects of preservatives on the stability of complex enzyme were studied.The results showed that the optimal process condition for producing mannooligosaccharide by complex enzyme was as follows:30 g/L konjac gum was hydrolyzed by reAuMan5AN3C3 and reAuCel12A with proportion of 1∶1.5 (former was 60 U/g for konjac powder)at 60 ℃ for 6 h.The hydrolysis rate could reach 65% under these conditions.The results from thin layer chromatography analysis showed that hydrolyzates of konjak gum mainly were oligosaccharides larger than biose,which were between biose and hexose.There was no monosaccharide.In the case of presence of preservative,the residual activities of complex enzyme were both above 85% after storage at room temperature for two months.This study provided a theoretical foundation for the industrial production of mannooligosaccharide by enzymatic method.
  • Food and Fermentation Industries. 2015, 41(8): 111.
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    Mustard Q123 (Brassica juncea) was used as raw material to make pickles by natural,direct vat set and aged brine fermentation.The quality differences on sensory,chemical,microbiological indicators and volatile flavor compounds among the three kinds of pickles were analyzed.Results showed that the change tendency of these indicators in the three kinds of pickles during fermentation process always kept consistent.The ripe pickle produced with direct vat set fermentation possessed suitable acidity,golden yellow color,crisp taste,low nitrite content,relatively high content of vitamin C,abundant volatile flavor compounds and short fermentation period,so its quality was better than that of the other two pickles produced with natural and aged brine fermentation.Furthermore,the content of lactic acid bacteria of the pickle with direct vat set fermentation was high,which could restrain growth of other organisms.
  • QIU Song-shan,WANG Yan-an,LIN Meng-hong,JIANG Cui-cui,Li Chun-hai
    Food and Fermentation Industries. 2015, 41(8): 117.
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    In this paper,litchi processing residues was fermented with the lipid-producing rhodotorula glutinis to produce microbial lipid.The optimal seeding time and volume of rhodotorula glutinis seed broth in flask were studied.Meanwhile,the process of microbial oil production was optimized by single factor experiment and orthogonal experiment to obtain the optimal fermentation conditions,and the physicochemical properties of the oil were analyzed.Results showed that the capacity of lipid production of 378.3 mg/L was achieved under the optimal conditions as follows:24 h of seeding time,40 mL/250 of liquid volume in flask,8°Brix of initial sugar concentration,5.5 of initial pH,10% of inoculum,and 96 h of fermentation time.Meanwhile,the biomass reached 15.670 g/L and the oil content was 2.414%.The microbial oil was yellow transparent liquid with average relative density of 1.104 4 g/L and refractive index of 1.459 0.Its acid value was 5.39 (mg/g),the iodine value was 101.8 (gI2/100 g),and the peroxide value was 9.44 (mmol/kg).The production of microbial oil with litchi residue reached the dual purpose of waste utilization and energy development and had the broad application prospect.
  • LI Chen,LI Jian-bin,Wei Juan,LIANG Chao-yu,ZHAO Yu
    Food and Fermentation Industries. 2015, 41(8): 123.
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    Using molasses alcohol distillation wastewater as substrate,the regeneration of copper-loaded activated carbon was investigated,and the optimum regeneration condition was established by orthogonal test.The optimal condition for catalyst regeneration was as follows:elution impregnation for 8 h,activation at 300 ℃ for 4 h,and ultrasonic treatment for 6 h.Use After the molasses alcohol distillation wastewater was treated with regenerative catalyst obtained under the optimum regeneration condition for 8 h,the removal rate of COD was 76.81%,and the decolorization rate was 71.44%.
  • ZHENG Xiao-jie,LIN Sheng-li,Nie Xiao-hua,XU Dan
    Food and Fermentation Industries. 2015, 41(8): 127.
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    Maillard reaction products from xylose-chicken bone hydrolysate system were prepared to investigate the effect of temperature on their spectral characteristics and volatile compounds.Along with the heating,the increases in the absorption and fluorescent intensity were observed.Compared with the control,the types and contents of aldehydes,ketones and esters in maillard reaction products were increased.The heterocyclic compounds were also detected in maillard reaction products.3-ethylfuran and 2,6-dimethylpyrazine might contribute to the flavour formation of the products.
  • GE Han-jing
    Food and Fermentation Industries. 2015, 41(8): 133.
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    135 yeast strains were isolated from all stages of spontaneous fermentation with No.8 Hutai grape,which is the unique species in Xi' an.And then,strain S37,which was suitable for the production of wine,was isolated after 3 screening cycles,and identified as Saccharomyces cerevisiae by phenotypic and molecular biological identification.Additionally,the fermentation conditions of strain S37 were studied.The results showed that the optimal fermentation condition was 24 ℃ and pH5.0.Furthermore,the aroma,taste and many other properties were closed to those of commercial yeast EC1118,some were even better.Therefore,strain S37 has application potential in wine brewing industry.
  • ZHANG Xuan,TANG Dao-bang,CHEN Zhi-yao,ZHANG You-sheng,Chen Jing-rong,LIU Xue-ming
    Food and Fermentation Industries. 2015, 41(8): 140.
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    In order to increase the degradation of chicken blood hemoglobin (Hb) by Aspergillus niger,the effects of sucrose,Hb,MgSO4,KH2PO4 and NaCl were optimized through orthogonal test.The results showed that the most important factor affecting the degradation rate of chicken blood hemoglobin was Hb concentration,followed by sugar,while the other three factors had less effect on the degradation of Hb by Aspergillus niger.The degradation rate of Hb reached the maximum when the culture medium was composed of sucrose 20 g/L,Hb 8 g/L,MgSO4 0.4 g/L,KH2PO4 2 g/L,and NaCl 5 g/L.On the basis of the optimized medium,the degradation rate of Hb attained its maximum after fermentation at 30 ℃ for 7 days with the inoculum size of 9% and loading volume of 30 mL/250 mL.After the optimization of fermentation process,the Hb degradation rate in fermentation medium increased from 28.2% to 46.8%.
  • XU Ju-fei,KANG Jiang,GU Fang-hong,WANG De-liang
    Food and Fermentation Industries. 2015, 41(8): 146.
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    Malt solubility is a significant determination index.The key malting parameters affecting malt solubility include barley steeping degree,germination temperature,searing temperature and drying temperature.Import barley was used as raw material,and single factor experiments were used to study barley malting.Fluorescent staining and ice emission scanning electron microscope were used to to observe malt endosperm dissolving conditions and thus determine malt solubility.Response surface design was used to optimize barley malting process.The experimental results showed that germination temperature was the main factor,and the grade of the factors influencing malt solubility was as follows:B(germination temperature) > D (drying temperature) > C (searing temperature) > A (steeping degree).Optimized barley malting conditions for the best malt solubility were as follows:the barley steeping degree was 46 %,germination temperature was 16 ℃,searing temperature was 48 ℃,drying temperature was 78 ℃.After optimization of malting process,malt solubility was increased from 81% to 87 %.
  • TANG Ke,WANG Bei,MA Yue,XU Yan,LI Ji-ming
    Food and Fermentation Industries. 2015, 41(8): 153.
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    Vidal ice wine samples fermented with 5 species of yeast strains at 3 different temperatures have been analyzed for organic acids by reversed-phase high performance liquid chromatography.The results showed that 10 organic acids were qualitatively and quantitatively determined in Vidal ice wines and as the most abundant amino acids,lactic acids accounted for 50% of the total organic acids content,followed by tartaric acid,citric acid,acetic acid and succinic acid.Concentration of maleic acid was the lowest,which was almost at trace levels.Low fermentation temperature was advantageous to the control of acetic acid,and the content of acetic acid was lower in different fermentation temperature by ST yeast.From the results of two-way analysis of variance,the acetic acid level was significantly influenced by both temperature and yeast species.
  • LI Yan-hua,WANG Wei-jun,MENG Yue-cheng,CHEN Jie
    Food and Fermentation Industries. 2015, 41(8): 159.
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    A method was developed for determining methyl 2-methylacrylate (MMA) in dairy product using solid-phase microextraction (SPME) with gas chromatography-mass spectrometry (GC-MS).The detecting parameters were optimized and internal standard (4-methyl-2-pentanone) method and the external standard calibration curve method were established.The best detecting conditions were:fiber type divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS),temperatures of equilibration and extraction were 80 ℃,extracting 30 min,and reconstituted milk volume 50 mL in a 100 mL vial.Good linear correlations were obtained.The linear range was 10 ~ 200 μg/ L.The average recoveries (%) ranged from 87.5% to 109.3% and their relative standard deviations were between 3.1 and 5.3%.The method can be used to determine the content of MMA in dairy product.
  • DING Hao-chen,Ruan Dong-na,JIANG Yin-mei,XU Gang,HUANG Jian-lian
    Food and Fermentation Industries. 2015, 41(8): 163.
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    The similarities and differences on volatile flavor of 4 kinds of cooked sea fish surimi including A.argentatus,P.edita,N.virgatus and Exocoetus were studied.The volatile components were identified by HS-SPME-GCMS and analyzed with ROAV.Overall odor was discriminated and evaluated by electronic nose and sensory odor evaluation.The key odor components of 4 kinds of cooked surimi were mainly aldehydes and sulfoether compounds.The key odor components of cooked P.edita surimi included dimethyl disulfide,3-methyl-butanal etc.The key odor components of cooked N.virgatus surimi and cooked Exocoetus surimi included dimethyl disulfide,dimethyl trisulfide and other aldehydes.But sulphur compounds were absent from the volatile flavor components of cooked A.argentatus surimi.Electronic nose analysis indicated that the response radar chart of cooked P.Edita surimi was distinctive.Furthermore,the response values of sensor array to cooked P.edita surimi were significant higher than the other samples.Results of PCA and LDA showed that there was similarity on cooked odor between A.argentatus surimi and N.virgatus surimi.The difference between P.edita surimi and Exocoetus surimi was significant.However,sensory odor evaluation showed that cooked P.edita surimi had a better odor,while the fat odor and unpleasant-fishy odor in cooked Exocoetus surimi were stronger.The intensity profiles on odor descriptions of A.argentatus surimi and N.virgatus surimi were similar.
  • XU Yong-xia,ZHANG Chao-min,ZHANG Ying,LI Xue-peng,SUN Hong,LI Jian-rong
    Food and Fermentation Industries. 2015, 41(8): 170.
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    The volatile odor changes in turbot during refrigerated storage at different temperatures were detected by electronic nose.The electronic nose technology for evaluating the turbot freshness was established by principal component analysis (PCA),loading analysis (LA) and cluster analysis (CA) method.The analysis also included total number of bacterial colony and TVB-N content.The results showed that smell changes during refrigerated storage at 0 ℃ and 4 ℃ could be well distinguished by PCA,LA and CA.Inflection points were 20 d and 16 d respectively.The predict shelf life by electronic nose was consistent with total bacterial count and TVB-N analysis prediction.Therefore,electronic nose technology can be used in identifying the freshness of turbot during refrigeration.
  • LI Ya-shuang,LIU Jie,BAO Ying,LIU Chun-lan
    Food and Fermentation Industries. 2015, 41(8): 175.
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    Crude polysaccharide was extracted from Brassica rapa L.After acidic ethanol fractionation,DEAE-52 and Sephadex G-100 gel filtration,WJdp4-b was obtained.Its homogeneity is confirmed by chromatography on Sepharose CL-4B and HPLC.By means of HIO4 oxidation,Smith degradation,methylation,partial acid hydrolysis,paper chromatography,GC,IR,NMR,and GC-MS analysis,the structure of WJdp4-b was identified.Its average molecular weight is 10 840.WJdp4-b was composed of rhamose (Rha),arabinose (Ara),mannose (Man),glucose (Glc) and its molar ratio is 1∶1∶ 10∶ 9.Its main chain is mainly made up of(1→4) Glc;Glc are substituted at 6-O and on average there were 12 branches among 17 main chain residues.The side chain was composed of(1→4) or(1→5)-Ara,(1 →3),(1→6)-Man.The terminal residue was composed of Rha,Glc.
  • GUO Jin-ying,LI Tong-hui,ZHU Bei-ru,REN Guo-yan,CUI Guo-ting,LI Zheng-jun,FENG Hui-min
    Food and Fermentation Industries. 2015, 41(8): 181.
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    This study aimed to compare and analyze the nutrient contents of cultured and wild Nostoc flagelliforme.Crude protein,fat,polysaccharide were measured in the cultured and wild Nostocflagelliforme were analyzed.The contents of amino acids,Ca,Mg,Cu,Zn Sr,Fe and Se were determined using HPLC and ICP-OES.The results indicated that crude protein and fat contents accounted for 26.17% and 0.23% (w/w) in cultured Nostoc flagelliforme,while 23.05% and 0.31% in the wild Nostoc flagelliforme.Exopolysaccharide contents reached 36.95 mg/L under cultivated conditions.Capsular polysaccharide contents in cultured and wild Nostoc flagelliforme were 15.03 mg/kg and 13.84 mg/kg,respectively.The total amino acids contents were 21.99 mg/g and 21.20 mg/g,essential amino acids contents were 11.73 mg/g and 7.97 mg/g.The elements of Ca,Mg,Cu,Zn,Sr,Fe,Se in the cultured Nostoc flagelliforme were rich,and Mg,Cu,Zn,Se were 2.9,3.4,3.8,and 1.6 times higher than that of in wild one.In conclusion,the cultured Nostocflagelliforme can secrete more exopolysaccharide.The contents of crude protein,fat,protein,amino acid,Ca,Sr,Fe were similar with wild Nostoc flagelliforme.Cultured Nostoc flagelliforme has great prospects for development.
  • WAN Yu-wei,NIU Li-ying,CHEN Ji-luan
    Food and Fermentation Industries. 2015, 41(8): 186.
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    In order to research the influence on purple potato juice' s quality during processing,the fresh purple potatoes were sliced,color fixated,blanched,enzymolysis,and pasteurized or autoclaving sterilized.Free amino acids,free sugars,total anthocyanidin and soluble protein content were analyzed after squeezing.Results showed that free amino acids,free sugars and anthocyanidin reduced significantly after color fixing and blanching treatment.However enzymolysis increased the contents of them in juice.In addition,both pasteurization and autoclaving sterilization could reduce the content of free amino acids,free sugars and anthocyanidin in purple sweet potato juice,while more compositions were lost by autoclaving sterilization than pasteurization.Moreover the water-soluble protein decreased during the processing,and only 20 % of fixed color remained after hot blanching.
  • XIE Jing,YANG Xi
    Food and Fermentation Industries. 2015, 41(8): 192.
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    In order to improve the quality characteristics of Trichiurus lepturus during chilled storage,Trichiurus lepturus during 4 ℃ cold storage were selected as controls,HHP (290 MPa,6 min),HHP (290 MPa,6 min) combined with three different compound antioxidants (2.5 % Carboxymethyl chitosan/0.05 % Vitamin E,2.5 % Carboxymethyl chitosan/0.25 % water-solubility rosemary extract,0.05 % Vitamin E/0.25 % water-solubility rosemary extract) to pretreated Trichiurus lepturus.To evaluate the preservation effects of Trichiurus lepturus during 20 days at 4℃,sensory evaluation,total bacteria count,physicochemical (total volatile basic nitrogen,the thiobarbituric acid,acid value and water holding capability) were periodically analyzed.Results showed that the shelf-life of the controls were 6 days,the shelf-life of Trichiurus lepturus pretreated by HHP were 12 days,the shelf-life of Trichiurus lepturus pretreated by HHP conbined with natural antioxidants were about 18days.The total bacteria count,TVB-N,TBA,acid value of samples pretreated by natural antioxidants and HHP which were lower than those of HHP groups were significantly lower than those of the controls.Among them,the preservation effects of the samples dipped in 0.25% water-solubility rosemary extract +0.05% Vitamin E had the best preservation effects,which could suppressed the growth of spoilage microorganisms effectively,slowed down the decomposition of protein,inhibited lipid oxidation and hydrolysis significantly,and maintained the good quality of Trichiurus lepturus effectively.
  • HU Zhong-hai,SUN Qian,MA Ya-qin,HAN Zhi,ZHU Pan-pan,WU Hou-jiu
    Food and Fermentation Industries. 2015, 41(8): 198.
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    This study aimed to investigate the effect of freezing rates on nutrition quality,color and texture of Satsuma mandarin segments.The comprehensive assessment data was carried out using grey correlative degree analysis.The results showed that citrus segments quality was affected by freezing rates to different extents.The most suitable rate for Satsuma mandarin is 0.36℃/min (freezing 91min under-50 ℃).Under this condition,grey correlative degree between the observed and desired quality was the highest.It indicated that citrus segments after freezing for 91min under-50 ℃ had higher soluble solids,higher vc,higher reducing sugar,lower titratable acid and minimal color and texture change.
  • MA Xuan,ZHU Xuan,ZHAO Ya-ting,DING Lan,FANG Chen-lu
    Food and Fermentation Industries. 2015, 41(8): 203.
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    Xinjiang " Saimaiti" apricot fruits were used as experimental material.They were divided into three different maturity based on the yellowish:Ⅰ (colored area < 50%),Ⅱ (colored area 50% ~ 80%) and Ⅲ (colored area > 80%).The fruit were stored at 4 ℃,RH 90% ~95%.The relationship between the tissue structure and wooly breakdown at different maturity of apricot fruits were studied by extracting the juice and evaluating the fruit firmness,soluble solids,titratable acidity every 7 days.The changes of tissue structure at the seventh day,the twentyfirst day and the thirty-fifth day of cold storage of apricot fruit were observed by paraffin section.The results showed that epidermal cell of maturity in group Ⅲ apricot fruit was broken,the pulp cell began to disintegrate,intercellular space expanded,and tissue structure was decomposed finally after the twenty-first day of cold storage.Group Ⅲ apricot fruit was the earliest and the most serious showing wooly breakdown,group Ⅱ was the second,group Ⅰ was the most trivial.It is suggested that maturity is one of the important factors affecting the occurrence of breakdown in apricot fruit.
  • ZENG Yuan,JIA Xing,WEI Liang-dong,WAN Nai-fu
    Food and Fermentation Industries. 2015, 41(8): 208.
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    The purpose of this article is to select spice oils that can reduce content of acrylamide generated in fried beef.In addition,the relationship between antioxidant activity and the content of acrylamide as well as oxidation inhibition mechanism were discussed.Finally,response surface methodology was applied to optimize fried beef processing.The results showed that:rosemary essential oil was chosen as the antioxidant and it reduced acrylamide content.The correlation between antioxidant activity and content of acrylamide was very significant,especially the scavenging rate of superoxide anion radicals,the correlation coefficient was 0.909.The mechanism of rosemary oil in reducing antioxidation activity is by 3 ways:competing with asparagine,capturing carbonyl compounds and its oxidation products,and reacting with acrylamide directly.The optimum conditions were as follows:soaking solution pH 6.90,the frying temperature 198 ℃,the frying time 1.6 min,acrylamide levels was 48.5 μg/kg.The color,flavor and the sense evaluation of dried beef were not affected significantly.
  • Food and Fermentation Industries. 2015, 41(8): 215.
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    A method using HPLC to detect the content of biogenic amines including tryptamine,phenylethylamine,putrescine,cadaverine,histamine,tyramine and spermidine in Sichuan-style sausage from traditional natural fermentation during processing and storage was developed.The results showed that only tryptamine,phenylethylamine and spermidine were found in meat raw materials,but 7 kinds of biogenic amines were found after storage for 120 days,Except spermidine,the content of 6 kinds of biogenic amines including tryptamine,phenylethylamine,putrescine,cadaverine,histamine and tyramine were significantly increased during processing(P < 0.01).The mainly biogenic amines in traditional natural fermentation Sichuan-style sausage were tyramine,histamine,cadaverine and putrescine with contents of 153.06,127.90,132.46 and 92.83 mg/kg respectively.These four kinds of biogenic amine accounted for 89.66% of the total biogenic amines.The contents of biogenic amines including tryptamine,phenylethylamine,histamine and tyramine in traditional natural fermentation Sichuan-style sausage were less than 20 mg/100 g.Amount of biogenic amines changed significantly during processing and storage (P < 0.01),wherein the total amount was less than 1 000 mg/kg.
  • GU Xia,YAN Tian-long,DENG Wei-ze,LI Ming-yuan
    Food and Fermentation Industries. 2015, 41(8): 220.
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    In this study,changes in moisture content,acid value,peroxide value,resiniferatoxin,compositions and relative amount of fatty acids of butter hotpot seasoning were investigated during cold storage at 4 ℃.The results showed that the water content,acid value,peroxide value increased with the cold storage and the oxidation speed of oil was accelerated.The content of resiniferatoxin decreased during cold storage and its degradation rate gradually slowed down.The contents of the unsaturated fatty acid decreased while the contents of lower fatty acid increased.These results indicate that control the fatty acids change during cold storage can help the quality and shelf life of butter hotpot seasoning.
  • Food and Fermentation Industries. 2015, 41(8): 224.
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    In order to improve the extraction rate of polysaccharides,the response surface method was used to optimize the extraction of polysaccharides from Collybia radicata.Extraction temperature,time,extraction times and ratio of water to material were used as factors in single factor experiments.Based on the single factor experiment,threevariable and three-level Box-Benhnken experiments were used to obtain optimum extraction conditions of polysaccharides.Results show that response surface method for the optimization of the extraction of polysaccharides from Collybia radicata is realistic and practical.The optimum conditions of extraction technology are three times extraction,84 ℃ extraction temperature,2.3 h extraction time,and a water:material ratio of 20∶1 (mL∶ g).Under optimum conditions,the extraction rate of polysaccharide reached 5.85%.The study provides basic information for its purification and industrial production.
  • Food and Fermentation Industries. 2015, 41(8): 229.
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    Citrus seeds contain 38.9% ~ 58.5% oil has great nutritional value and wide application potential after refining.This paper compared the qualities of extracted method of supercritical CO2 fluid,ultrasonic assisted solvent,cold-pressed and hot-pressed,respectively.Several properties were compared including the initial physical and chemical indicators,self-oxidative capacity,limonoids content and fatty acid composition.All oil extracts were satisfied hygiene standards of edible vegetable oils.Acid value by supercritical CO2 fluid was higher than others (P < 0.05).The limonoids content extracted by supercritical CO2 fluid was much higher than by other methods.The main components of fatty acids were palmitic acid,stearic acid,oleic acid,linoleic acid,etc.higher content of dohomo-γ-linolenic acid was found in oil extracted by supercritical CO2 fluid.Based on storage capacity test,we predicted that cold-pressed oil can be stored for 92.4 d and oil extracted with supercritical CO2 fluid can be stored for 41.7 d under 20 ℃.According to our comprehensive analysis,supercritical CO2 fluid extraction is the best,but it needs deacidification to improve the storage capacity.
  • LEI Song,DOU Jian-zhou,MEI Zhi-fang,YANG Pin-hong,WANG Bo-hua
    Food and Fermentation Industries. 2015, 41(8): 234.
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    Silver carp,one of the four famous freshwater fish,has limited commercial value due to its strong earthy taste and lots of fish bones.However,its salt soluble proteins can be extracted and has higher economic value.Therefore,optimization of extraction conditions of silver carp salt soluble protein and their processing properties are studied in this paper,The best extraction conditions are 0.8 mol/L NaCl concentration,pH 6.0,extraction time 30 h and solid-liquid ratio 1∶5.Under the above conditions,salt soluble protein concentration is 2.602 4 g/100 g and extraction yield is about 80%.Silver carp thermal induced salt soluble protein gel has good water retention,water holding capacity can be up to 98.24%,the best inducing temperature is 80 ℃.Phosphate can help water retention of the gel.Silver carp salt soluble protein has good emulsification ability and its emulsifying activity (EAI) is 19.47 m2/g,emulsion stability (ESI) is 89.45%.Therefore,the gel can effectively improve the quality of meat products.The study provides a new application of silver carp.
  • Food and Fermentation Industries. 2015, 41(8): 239.
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    Silkworm chrysalis was selected as the raw material and chrysalis oil yield as the research indicator.The technology of chrysalis oil extraction was studied by single factor experiment and orthogonal test.The acid value,iodine value and color of refined oil were tested and the fatty acid composition was analyzed.Results showed that the optimum conditions were n-hexane 8 mL/g,extract temperature 40 ℃,extract time 120 min,oscillation frequency 120 r/min.Under these conditions,chrysalis oil yield and refined oil were 24.49% and 72.33%,respectively.The color of chrysalis oil was transparent and light yellow color.The content of unsaturated fatty acid and α-linolenic acid were 75.40% and 54.51%,respectively.
  • LI Xue-peng,CHEN Gui-fang,YI Shu-min,ZHU Jun-li,LI Ting-ting,LI Chun,LI Jian-rong
    Food and Fermentation Industries. 2015, 41(8): 244.
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    Microbial activities is one of the major factors of food deterioration.The studies showed that bacterial quorum sensing plays an important role on food spoilage.This article summarized the mechanism for generation of quorum sensing system and its related researches.In particularly,the effect of bacterial quorum sensing on spoilage of foods,such as milk and milk products,meat and meat products,aquatic products,fruits and vegetables,was analyzed.Furthermore,the new strategy for food preservation based on quorum sensing inhibitors was briefly discussed.This paper will provide the theoretical basis for the study of food deterioration mechanism and the development of preservation methods derived from quorum sensing.
  • Food and Fermentation Industries. 2015, 41(8): 251.
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    This paper describes the intelligent sensory applications such as electronic nose and electronic tongue,electronic teeth,electronic eyes and other equipment anthropomorphic equipment in engineering rice research and manufacture.Intelligent sensory evaluation of R&D personnel can assist the development of improving engineering rice direction,optimizing engineering rice recipe,controlling engineering rice security and authenticity of ingredient and testing engineering rice product shelf life.The novel intelligent sensory evaluation promote the development and innovation of engineering rice.
  • LIU Xiao,XIE Jing,HUANG Shuo-lin
    Food and Fermentation Industries. 2015, 41(8): 255.
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    Recently,with constantly grew of live fish market,more and more attention had been paid on the research of keeping fish alive transportation.Current fish transportation techniques such as water purification,aerobic,drop temperature and anesthesia were reviewed.The main factors which influenced transportation,such as aquatic species,water quality,temperature,and forcing stress were introduced in the paper.The stress response and relevant research methods in the process of fish transportation through behavioral and physiological aspect were expounded.Finally,existing problems of the transportation were analyzed in order to put forward research direction.The purpose of this paper was to provide reference for research and improvement in transport alive fish.
  • LIU Ke-wei,FU Mao-run,LIU Tao,ZHANG Xiao-hui,XU Min-hui
    Food and Fermentation Industries. 2015, 41(8): 261.
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    Due to its excellent properties of broad spectrum,high efficiency,low toxicity and little drug resistance,nano materials used as antimicrobial agents in post-harvest fruits and vegetables has become a hot research topic.Different aspects including microbial morphology damage,permeability of microbial cell membrane,and cellular respiration influenced by nano materials were summarized in the paper.Then,two mechanisms including reactive oxide stress and ions dissolution effect were summarized.Furthermore,the prospects of research trend in mechanism of nano material inhibiting microbial at molecular level was prospected.
  • YE Yong-li,HU Xiao-peng,CHEN Shi-en,HE Xin-rui,MA Zhong-ren
    Food and Fermentation Industries. 2015, 41(8): 267.
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    As one of the contaminants during the food processing,how to improve the detection sensitivity of acrylamide and reduce its production in foods has become one of the hot spots in the field of food safety.In recent years,remarkable progresses have been made in new methods and technologies for determining acrylamide in many industries.In this paper,mainly used methods and applications are reviewed,and hope it can provide a new train of thought for the analysis of acrylamide in foods.