25 January 2012, Volume 38 Issue 01
    

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    Food and Fermentation Industries
  • Zhang Bo, Guan Zheng-bing, Xie Guang-fa, Lu Jian, Yu Pei-bin
    Food and Fermentation Industries. 2012, 38(01): 1-7. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.026
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    During the process of wheat Qu-making,a series of metabolic reactions originated from microorganisms inoculated naturally occur to provide various enzymes and flavour compounds precursors for fermentation of Shaoxing Rice Wine.According to metaproteomics,two-dimensional electrophoresis(2-DE) and tandem mass spectrometry(MS/MS) methods were used to analyze the extracts of "wheat Qu" during the six making-stages(including starting period,early-fermentation period,warming period,high-temperature period,cooling period and mature period).By comparative analysis of the 2-DE maps by using the PDQuest software,12 spots with dramatic variations of expression level were found and subsequently identified by MALDI-TOF/TOF MS.Among the 7 protein spots which were identified successfully,there were 4 spots from microbes,including the coagulation factor with 5/8 type domain protein(Amycolatopsis mediterranei U32),the malate dehydrogenase(Micromons sp.RCC299),carboxypeptidase T precursor and glucose-6-phosphate isomerase(Saccharopolyspora erythraea NRRL 2338).The other 3 protein spots were the hypothetical protein(Ectocarpus siliculosus),xylanase inhibitor protein and class II chitinase(Triticum aestivum).
  • Wang Tao, You Ling, Zhao Dong, Feng Rui-zhang, Wang Song, Feng Xue-yu, Zhang Yun
    Food and Fermentation Industries. 2012, 38(01): 8-14. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.027
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    In order to better understand the correlation between bacterial community of high-temperature DaQu and air of Qu workshop of multi-grain strong-flavor liquor factories,and to collect potential microbial resources,we employed modified nutrient agar medium and Gogan-I medium for isolation and cultivation.Then 16S rRNA gene sequences of 217 non-redundant bacterial isolates were be analyzed,and abilities of lignocellulose-degrading,saccharifying,7 % ethanol and pH 4.5 tolerance of these isolates were be screened,too.Phylogenetic analysis based on 16S rRNA gene sequences showed that Bacillus is the most dominant genera of two bacterial community,and Staphylococcus and Streptomyces is following dominant genera of high-temperature DaQu and air of Qu workshop,respectively.These strains with abilities of lignocellulose-degrading,saccharifying,ethanol tolerance and structure of two bacterial communities were comparatively analyzed.The results indicated that Qu workshop bacterial community and climatic factors remarkably influenced the structure and function of high-temperature DaQu bacterial community.
  • Feng Jie, Zhan Xiao-bei, Ji Xin-yue, Zheng Zhi-yong, Wang Dong, Zhang Li-min
    Food and Fermentation Industries. 2012, 38(01): 15-19. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.021
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    In the studies of the effects of various levels of NaCl plus addition of amino acids on HEMF production by Candida etchellsii,we used the 2-step process with initial 200 g/L NaCl and subsequently 220 g/L NaCl after 40 h of fermentation with the addition of alanine,arginine and glycine(both 1.67 g/L) in the medium.The shake flask fermentation results indicated that 110.74 mg/L of HEMF was produced by the C.etchellsii yeast.Results from the 7 L fermentor experiments showed that HEMF production reached 121.51 mg/L,a 21.2-fold increase above the control which contained 200 g/L sodium chloride and no amino acid.The 2-step,2-level NaCl with various amino acid additives process in the fermentation medium significantly improved the production of HEMF by the C.etchellsii yeast.
  • Sun Jia-kai, Wu Xiao-jiao, Wang Jing, Xu Qing-yang, Xie Xi-xian, Chen Ning
    Food and Fermentation Industries. 2012, 38(01): 20-24. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.022
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    The effects of phosphate on the fermentation of L-isoleucine was studied with L-isoleucine producing strain E.coli TRFC.The effects of initial phosphate concentration in the fermentation process on biomass,the specific growth rate,yield of L-isoleucine,concentration of byproduct acetic acid and ammonium were studied by carrying out fed-batch fermentation of E.coli TRFC in 30-Liter fermentor.The results showed that maintaining the initial phosphate at the concentration of 2g/L and adding 2g/L phosphate into the broth in the late period of fermentation could effectively slow down bacterial recession.The biomass and production of L-isoleucine increased by 24.5% and 12.7%,while the amount of by-product acetic acid decreased significantly.
  • Zuo Zhi-han, Zhao Hai-long
    Food and Fermentation Industries. 2012, 38(01): 25-29. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.001
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    Clavulanic acid positive regulation gene claR of Streptomyces clavuligerus was cloned.claR expression plasmid pSET152-claR was constructed and transformed into S.clavuligerus by conjugation.The attP site in pSET152-claR could inserted into attB site of S.clavuligerus.Thus an extra copy of claR was inserted into the chromosome of S.clavuligerus.The obtained mutant S.clavuligerus: claR was cultured in the shaken flask,and the production of clavulanic acid was analyzed by HPLC,the yield of clavulanic acid was 1.86 times compared with S.clavuligerus.
  • Ke Tao, Xiong Lan, Zhang Nai-qun, Ma Xiang-dong, Hui Feng-li, Niu Qiu-hong, Yang Guo
    Food and Fermentation Industries. 2012, 38(01): 30-35. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.002
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    Based on the codon bias of Pichia pastoris,the gene encoding a thermoacidophilic α-amylase BD5088 was modified for the optimal expression in P.pastoris.The gene was cloned into the vector of pPIC9K to produce the recombinant vector pPIC9K-BD5088 and then transformed into chromosome of P.pastoris GS115 strain.Regulated by the α-factor,promoter of AOX1 gene and termination signal of yeast genomic,the recombinant α-amylase PrBD5088 was expressed and secreted into the culture with a max yield of 200 mg/L after 5 days induction.Comparing to the recombinant amylase ErBD5088 expressed by E.coli,the optimum temperature of PrBD5088 increased from 80℃ to 90℃.Although the optimum pH of PrBD5088 was same to ErBD5088,but PrBD5088 have more broad stable range of pH than ErBD5088.More than 50% of its maximal activity was retained in the pH 4.0~8.0 of PrBD5088 and pH 5.0~7.0 of ErBD5088.The PrBD5088 is so thermostable that over 80 % of its activities was retained after disposed at 100℃ for 1 h.But the half-life of ErBD5088 at 100℃,pH5.6 was only 20 min.High concentration of Zn2+ and Ca2+ slightly increased its activity,while Cu2+ partially inhibited its activity at high concentration.SDS-PAGE result showed the molecular weight of PrBD5088 was about 56 ku,which was bigger than the calculated molecular mass 49 ku of ErBD5088.PrBD5088 is not modified by N-linked glycosylation but by O-linked glycosylation.The O-linked glycosylation had influence on thermostability,the optimal temperature and optimal pH range of PrBD5088.
  • Zhang Yan-ying, Xiao Dong-guang, Zhang Cui-ying, Wang Wen-yang, Wang Dong-sheng, Li Yue-qiang
    Food and Fermentation Industries. 2012, 38(01): 36-40. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.028
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    In order to obtain an engineering strain lacking antibiotic resistant gene with low-yield of higher alcohols,the plasmid pGAPZA with the gene encoding the Cre recombinase was transformed into the BAT2 deletion haploid mutants A8-B and C22-B.Then the G418 resistance gene(KanMX) was wiped off by Cre/loxp system from the mutants A8-B and C22-B,and Saccharomyces cerevisiae haploid mutants δBAT2a and δBAT2α were obtained.These two haploids were mated into a diploid δBAT2,which was verified to be genetically stable.Moreover,at the end of the liquor fermentation,the main fermentation profiles of the wild-type strain AY15,the parental strains A8-B and C22-B and the engineering strain δBAT2 were determined.The experiment results showed that the engineering strain δBAT2 was compared with the parental strains,weight loss of CO2 was higher,the residual total sugar was lower,ethanol was higher,yet the engineering strain was compared with the wild-type strain,the amount of isobutyl alcohol,isoamyl alcohol and the total higher alcohols were respectively reduced by 50.00%,33.40% and 30.57%.The engineering strain lacking antibiotic resistant genes with low-yield of higher alcohols was used as an excellent strain in the liquor industry.
  • Zhang Hui-juan, Wally Yokoyama, Zhang Hui
    Food and Fermentation Industries. 2012, 38(01): 41-45. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.003
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    This study investigated the effect of brown rice protein(BRP) and BRP hydrolysates(BRPH)on lipoprotein metabolism in Syrian Golden hamsters fed high-fat diets compared to casein.The supplements of brown rice protein and its hydrolysates reduced hepatic total lipid,total cholesterol and free cholesterol content of hamster.Meanwhile,the BRP and BRPH diet increased the fecal total lipid,total cholesterol and free cholesterol content.Moreover,BRPH significantly reduced the hamster body weight compared to control.
  • Wu Wei-wei, Long Xiao-hua, Liu Zhao-pu, Gao Ying-ying, Wang Chang-hai
    Food and Fermentation Industries. 2012, 38(01): 46-50. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.004
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    The effects of nitrogen in different format on the growth,biochemical composition,fatty acid composition and content of Phaeodactylum tricornutum and Tetraselmis Chui was investigated.The results showed that: with urea as nitrogen source,the biomass,growth rate,chlorophyll content,soluble sugar and soluble protein contents of Phaeodactylum tricornutum were the highest,followed by sodium nitrate and ammonium chloride as nitrogen source.However,with ammonium chloride as nitrogen source,total lipid content was the highest,and types of fatty acids were the most.Tetraselmis Chui with ammonium chloride as nitrogen source,the biomass,growth rate,chlorophyll content,soluble sugar and soluble protein contents were the highest,followed by urea,sodium nitrate.However,the total lipid content and fatty acid species reached their maximum with sodium nitrate as nitrogen source.
  • Bai Feng-ling, Zhang Bo-lin, Zhao Hong-fei
    Food and Fermentation Industries. 2012, 38(01): 51-56. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.029
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    Due to the lack of various biosynthetic pathways,lactic acid bacteria(LAB) are nutritionally fastidious and unable to synthesize some essential amino acids and vitamins,so that the high-density LAB cultures must depend on exogenous essential amino acids,vitamins and other growth factors.The growth kinetics of LAB were carried on the chemically defined media(CDM) of three kinds of nitrogen sources of minimal amino acids,all amino acids and soy protein hydrolysates(SPH).Results showed that: the growth μmax of Lactobacillus bulgaricus and Streptococcus thermophilus in the molecular weight less than 5ku SPH media were 0.215 h-1 and 0.262 h-1,respectively,were about 2 twice all amino acids.The cell density of two LAB in the SPH media were 4 times of all amino acids media.The media of all amino acids were low efficiency for L.bulgaricus and S.thermophilus,which growth maximum rates were 0.10 h-1 and 0.12 h-1,respectively,and the pH values of two kinds of cultures decreased only 4.5 to 5.0.Essential amino acids minimal medium can just meet basic requirements for growth of LAB,less than 5ku soy protein hydrolyzate played a significant role for promoting the proliferation of LAB than all amino acids.The oligopeptides in the SPH was the best nitrogen resource for LAB growth,rather than amino acids.Thus SPH can be applied as a kind of additional nitrogen resource ingredients to enhance growth rate of LAB.
  • Yuan Yue, Wang De-liang, Guo Li-yun, Lin Zhi-ping, Jiang Wei
    Food and Fermentation Industries. 2012, 38(01): 57-61. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.030
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    A strain which can produce antifungal protein was isolated from soil and was identified as Aspergillus giganteus,numbered AF-11,through morphologic observation and molecular biology experiments.It has been proven that the high activity and purity antifungal protein(AFP) which can obviously inhibit the growth of filamentous fungi such as Penicillium,Aspergillus and Fusarium,can be separated from the fermentation supernatant by using cellulose cation exchange column chromatography and gelatin column chromatography.
  • Wang Yuan-yuan, Cao Chun-hong, Yang Xu, Xiao Dong-guang
    Food and Fermentation Industries. 2012, 38(01): 62-65. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.031
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    Sugar molasses was used to culture the High nuclear Saccharomyces.The fermentation condition was studied.The optimum medium ingredients and culture condition were obtained as followed:the initial pH 5.0,the complex nitrogen sources of yeast powder and urea;by increasing the rate of the hydrolyzation of the sucrose,the logarithm period of NY-1 is prolonged and the cell density was also increased.The cell dry weight was up to 15.4g/L when the yeast was cultured in baffled flasks.
  • Fang Jian, Luo Xiao-xue, Zou Yu-lei
    Food and Fermentation Industries. 2012, 38(01): 66-71. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.032
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    The influence of different cellulose derivatives on properties of starch-chitosan edible films was studied.The structure properties of edible films were characterized by XRD,SEM and TGA.The results showed that the ensile strength of films decreased slowly and tensile elongation increased greatly as the CMCNa content increased.As MC was blended into the films,the ensile strength of complex films increased greatly and tensile elongation changed very little.Antibacterial properties of edible films on Staphylococcus aureus were better than on Escherichia coli.The results of XRD and TGA indicated that strong interaction existed among cellulose derivatives,starch and chitosan.
  • Tang Xiao-dan, Zhao Jin-liang, Yun Da, Hang Hai-yang
    Food and Fermentation Industries. 2012, 38(01): 72-76. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.013
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    The adsorptive thermodynamics and dynamics characteristics of Gynostemma polysaccharide on macroporous resin was studied.UV-visible spectrophotometry was used to detect the adsorptive equilibrium curve and adsorptive dynamic curve at different temperatures.The results showed that the adsorptive balance data were correlated well to Langmuir equation,second-order kinetic equation,Boyd film diffusion equation and kannan-sundaram equation.The relativities among them were all good.This adsorptive procedure was a non-spontaneous endothermic process of increased entropy.The rate of adsorption was mainly controlled by the intra-particle diffusion.
  • Xu Hong-xian, Duan Gang
    Food and Fermentation Industries. 2012, 38(01): 77-82. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.033
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    Viscosity is one key bottleneck of high gravity fermentation.The viscosity profiles of both conventional process and no-cook process are investigated using whole ground corn as the raw material.At very high dry solid concentration,it is found that during conventional hot cooking process,the gelatinization and liquefaction become very hard to handle while there is no viscosity issue for no cook process.In addition,the no cook process generate more ethanol than that from conventional process during high gravity fermentation;with the help of raw starch hydrolyzing enzymes,together with commercial dry yeast and temperature staging strategy,ethanol concentration was able to reach up to 20%(v/v) within 98h.
  • Liu Jian, Lin Ji, Lei Zheng-he, Lv Li-hua, Zhao Liang-qi
    Food and Fermentation Industries. 2012, 38(01): 83-87. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.034
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    The composition of organic acids in summer lees of fen liquor were analyzed via high performance liquid chromatography in this paper.Acidogenic bacteria were isolated from summer normal fermented grains and high acid fermented grains by flat crossed method.The influence factors of lees acidity were studied and fen liquor brewing process was optimized.Conclusion was: acetic acid and lactic acid were primarily organic acids in fermented grains;23 strains acetic acid bacteria and 5 strains lactic acid bacteria were isolated from normal fermented grains;23 strains acetic acid bacteria and 29 strains lactic acid bacteria were isolated from high acid fermented grains.Environmental temperature,moisture of fermented grains and amount of each urn packed greatly influenced the acidity of fermented grains.On this basis,suitable acid brewing conditions were obtained using the response surface method: with the environment temperature of 20 ℃,moisture of fermented grains of 51% and amount of each urn packed of 0.83 kg.The total acidity of fermented grains was 2.12(0.1 mol NaOH mL/g fermented grains),which was consistent with the model prediction of 2.07.The results provided scientific basis to solve the problem of high acid lees of fen liquor in summer.
  • Zhu Qiang, Xia Yan-qiu, Liu Shan-shan
    Food and Fermentation Industries. 2012, 38(01): 88-91. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.035
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    Effect of malt on Chinese rice wine production was studied by partially replacing wheat Qu with malt in Chinese rice wine fermentation.The results showed that the optimal ratio of malt and wheat Qu was 1∶ 1 and the total usage was 10% of raw material.The finished product was orange,aroma and light taste Chinese rice wine.It is also discovered that perlite and diatomite could effectively reduce the Chinese rice wine precipitation and the appropriate total usage was 2~3 g/L.In conclusion,adding malt in fermenting mash could reduce wheat Qu usage and improve Chinese rice wine quality and flavor.
  • Zhang Sheng-ke, Yan Shi-liang, Huang Hui-ying, Shi Xiao-li, Li Lin, Zhu Teng-yue, Cui Feng-xia
    Food and Fermentation Industries. 2012, 38(01): 92-95. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.023
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    Starting time of D-ribose single-stage continuous fermentation was detected.The effects of dilution rates and influent glucose concentration on single-stage continuous fermentation were also investigated.The results indicated that optimal productivity reached more than 4.0 g/L/h under the conditions of starting time 24h,dilution rates 0.06 h-1 and influent glucose concentration 200 g/L.The steady stage was obtained effects 48h of continuous fermentation,and various parameters held stable for 160 h.
  • Zhang Qing-qing, Lv Wen-wen, Tang Wen-jing, Xu Peng
    Food and Fermentation Industries. 2012, 38(01): 96-100. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.024
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    In order to reduce the production cost of γ-aminobutyric acid and make the best of bean dregs,the fermentation conditions for Monascus ZL307 to produce γ-aminobutyric acid with bean dregs as raw material in solid-state fermentation were optimized.Based on the study of single factor tests,we used Plaekett-Burman design to evaluate the influence of six related factors.Rice flour,MgSO4,KH2PO4 were affirmed the important factors in the medium.After identifying three critical factors,the optimal region of γ-aminobutyric acid production was found by steepest ascent approach.Then response surface methodology was also used to develop a mathematical model to identify the optimum concentrations of the key nutrients for higher γ-aminobutyric acid production,and to confirm its experimental validity.The results showed that the optimal fermentation conditions were as follows: bean dregs 12.28g,rice flour 7.72 g,(NH4)2SO4 0.20%,MgSO4 0.23%,KH2PO4 0.37%,CaCl2 0.25%,sodium glutamate 0.45%,initial water content 60%,initial pH 5.5,temperature 32 ℃.Under the optimum conditions,the yield of γ-aminobutyric acid reached 0.417 mg/g,which was 13.4% higher than before.
  • Wang Feng, Li Bao-guo, Shen Jiang, Su Shu-qiang, Zhang Ming-rong
    Food and Fermentation Industries. 2012, 38(01): 101-104. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.036
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    Drying method is used for dehydrating food for long-term preservation.Hot-air drying is not suitable for drying heat-sensitive food,and the product qualities is poor;vacuum freeze-drying is able to overcome these disadvantages,but to much time and low efficiency.This paper provides a new drying technology,controlled freezing-point drying.Taken carrot as the experimental material,the controlled freezing-point dried processing is studied.The result was compared with the vacuum freeze-drying and hot-air drying.The result were : at the pressure of 150 Pa,after 4 hours of drying,water content of carrot was 4.8%.The results also showed that rehydration was better,Vc and carotene in carrots were merely lost,the carrots kept nice color and appearance.Compared with freeze-drying,drying time significantly reduced.
  • Meng Li-xia, Liang Xin-quan, Li Min, Lu Ji-xin, Li Xian-neng, Li Jian-bin
    Food and Fermentation Industries. 2012, 38(01): 105-108. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.037
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    This paper mainly studies the high-temperature steamed sugarcane leaves For preparing xylan.There were four factors influenced extraction: pre-soaking methods,liquid-solid ratio,boiling time and temperature.The results show that: when the boiling temperature rose,the total sugar content increased;when boiling time extend,it increased firstly,then reduced;When the proportion of water to the sugarcane leaves increased,it increased and then decreased.This study also provided the best production technology conditions: the sugarcane leaves soaking for 12 h with water at 60 ℃,for 60 min,the proportion of the sugarcane leaves with distilled water 1∶ 10,then cooking at 126 ℃ for 60minutes.Scanning electron microscope(SEM) analysis was used to observe residue morphology before and after cooking.The results indicated that high-temperature cooking helped the dissolution of xylan.This result provided a basis to prepare xylo-oligosaccharides from xylan by enzyme in future.
  • Li Xin-hua, Jin Sheng, Zhang Li-li
    Food and Fermentation Industries. 2012, 38(01): 109-111. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.038
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    In order to solve the problem of the traditional sour liquid producing methods in the extraction ratio and quality,the experiment uses the fermented liquid of lactobacillus paracasei which is separated from the sour liquid of Sweet Potato,to replace the traditional sour liquid to separate mung bean starch.Through orthogonal test,we find out that the major factors that influence the effect of Lactobacillus paracasei are pH,temperature,proportion of solid and liquid in the defibrination and the volume of the fermented liquid.The sequence of the primary and secondary factors is the proportion of solid and liquid during the defibrination,temperature,the volume of the fermented liquid and pH.And the optimum parameters are that the volume of the fermented liquid is 33 %,pH is 7.5,the temperature is 60℃ and the proportion of solid and liquid in the defibrination is 1∶ 3.
  • Wu Hai-yan, Zhong Zhen-sheng, Gai Chun-hui
    Food and Fermentation Industries. 2012, 38(01): 112-115. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.039
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    Potato residue was generated during the process of potato starch production.It contains a lot of starch and dietary fiber and small amounts of protein.It is a valuable resource.In this paper α-amylase and glucoamylase were used to treat potato residue.And then the liquid portion was separated and fermented into edible protein.The solid part was bleached,modified to dietary fiber.Thus the potato residue was fully used.This study designed the process,selected the enzymes and optimized the process conditions.The protein and dietary fiber's physical and chemical properties were determined.
  • Li Yan, Zheng Xiao-jie, Huang Xue-fei, Xu Jing
    Food and Fermentation Industries. 2012, 38(01): 116-119. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.040
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    Technical parameters of ceramic membrane microfiltration of waste liquid in marinating were studied.The effect of pretreatment method,membrane pore size,operation pressure,operation temperature on membrane flux were studied.The best parameters were determined by orthogonal experiment: pretreatment by 300mesh screen,membrane pore size 0.22 um,operation tempreature 50℃,operation pressure 0.075 MPa.The recovery percent of ceramic membrane was 94.1% after wash by 0.75% NaOH and 0.5% citric acid.
  • Sun Li-ping, Zhang Hui-lin, Yang Mei-zhizi, Liu Yang-ming, Zhuang Yong-liang
    Food and Fermentation Industries. 2012, 38(01): 120-122,127. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.005
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    The content and composition of the polyphenolics in crystal pomegranate peels from Yunnan province was investigated,and the antioxidant activities were determined.The results showed that the polyphenolic content in crystal pomegranate peels was 99.60 mg/g(DW).P-coumaric acid,(+) catechin and protocatechuic acid were the dominate components,and their contents were 4.80,3.96 and 1.96 mg/g DW,respectively.The polyphenolic extracts showed significant reducing power,DPPH-scavenging activity,lipid peroxidation inhibition ability and nitrite-scavenging capacity.The antioxidant activities of the extracts were significantly positive correlated with polyphenolic content(P<0.01),and the determination coefficient(R2) were in the range of 0.888 1~0.995 8.
  • Cui Jie, Gu Xin, Huang Kun, Wang Wen-jiang, Li Di, Wang Jian-zhong
    Food and Fermentation Industries. 2012, 38(01): 123-127. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.006
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    The antioxidant activity of apricots prepared by two different methods were compared.The antioxidant activity of apricot SDF was investigated by four antioxidant assays,which contained total reductive ability,scavenging ability for DPPH radical(DPPH·),hydroxyl radical(·OH),and superoxide anion radical(O-2·).The results show that the two different SDF had certain antioxidant activity,and their antioxidant ability increased with the growth of SDF content.There were some differences in their infrared spectrum because of the different hydrolyzing degrees.
  • Wan Hong-gui, Zhang Bo, Wang Wen-jin, Wang Wen-juan, Miao Ling-ling
    Food and Fermentation Industries. 2012, 38(01): 128-132. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.012
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    The principle of simulated moving bed(SMB) is introduced in details.The main applications of SMB chromatography in the area of petrochemical,bio-separation and chiral drugs,the designation and optimization methods of its model,new operating methods and new pattern SMB system are also reviewed.Meanwhile,the trends and future research priorities of SMB chromatography are prospected.
  • Shi Yan-qiu, Xin Xiu-lan, Yuan Qi-Peng
    Food and Fermentation Industries. 2012, 38(01): 133-137. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.041
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    Lycopene is a red-colored carotenoid with specific application as feed supplement and nutrient in human diets because of its potential in alleviating chronic diseases such as certain cancers and coronary heart disease,as such it is considered to be a potent anti-carcinogenic agent and antioxidant.Blakeslea trispora is convenient for large-scale production in fermentor due to its high growth rate and low nutritional needs.This paper mainly discussed various of fermentation accelerant used on lycopene production by Blakeslea trispora.Accelerants are classified into eight major types according to the mechanism.
  • Sheng Xiao-xiao, Zhou Ru-jin, Tang Yu-bin
    Food and Fermentation Industries. 2012, 38(01): 138-142. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.014
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    Cellulose is a renewable resource that is abundant on the earth.At present,fuel alcohol produced by pretreatment,saccharification and fermentation from cellulosic materials is more economical and environmentally friendly than gasoline or other traditional energy types.Therefore,cellulose alcohol is being developed into a new kind of renewable energy.The adoption of cellulose alcohol production technology makes the utilization of many cellulose materials from agriculture and forestry waste easy and comprehensive.It not only establishes a sustainable biological energy development model,eases the growing energy demand,but also benefits the bio-energy development,economic construction and environmental protection.This paper will present a review concerning the present technology stages of cellulose alcohol and the future development trends at home and abroad.
  • Hu Xiao-liang, Zhou Guo-yan, Wang Chun-xia, Zhan Bo
    Food and Fermentation Industries. 2012, 38(01): 143-146. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.007
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    Sodium alginate is a natural existed polysaccharides.It is non-toxic and odorless,biodegradable,biocompatible,low cost,excellent dispersing and moisturizing,good in film forming,and has the function of antimicrobial.It becomes the hot research spot in the study of fruits and vegetables preservation.The mechanism of sodium alginate and its derivatives for fresh fruits were introduced.The application in the preservation of fruits was reviewed.Suggestions of future development were presented.
  • Ma Zhen, Li Lv-mu
    Food and Fermentation Industries. 2012, 38(01): 147-151. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.042
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    Nnatural preservative were attracted by more and more people;apple polyphenol is one of natural plant polyphenols which have effects on reducing lipid oxidation and inhibiting the growth of harmful micro-organism in meat products.It is used as the antioxidant,bacteriostasis and color protective agent.This review summarizes the composition of apple polyphenols as well as the effect and application in meat preservation.
  • Yu Ying, Li Ji-ming, Shen Zhi-yi, Jiang Wen-guang
    Food and Fermentation Industries. 2012, 38(01): 152-155. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.043
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    We used solid phase extraction and gas chromatography-flame ionization detector(SPE-GC-FID).Effects of different vintage technologies(pectases,yeasts,malolactic bacterias and fermentation temperature) on the formation of ethyl carbamate in Cabernet Gernischt wines during vinification were studied.The results showed the concentration range of ethyl carbamate in dry red wines was 7.00~26.80 μg/L,and the average was 17.90 μg/L.In dry white wines,the concentration range was 6.40~21.58 μg/L and the average was 11.35 μg/L.The ethyl carbamate is formed mainly from yeasts and malolactic bacterias during winemaking.The lower temperatures of alcoholic fermentation and the supplements of pectases before alcoholic fermentation could contribute to minimize the production of ethyl carbamate in wine.
  • Li Jun-yan, Qiu Kai, Zhong Qi-ding, Yu Xiao-jin, Ding Fu-qiang, Xiong Zheng-he, Zhang Ze-sheng
    Food and Fermentation Industries. 2012, 38(01): 156-160. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.044
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    A method of determination of available lysine in infant formula powder with 2,4,6-trinitrobenzene sulfonic acid(TNBS) was established.The ether usage,hydrolysis time and incubation time with TNBS were studied.Good linearity was obtained in the range of 0.02~0.12 mg/mL(r2=0.999 3);the determination limit was 3.72 μg/mL;and quantitative limit was 10.23 μg/mL and the sample spiked recoveries were in range of 86%~115% with the reproducibility RSD of 1.55%~5.57%.
  • Gu Yu-xiang, Ge Yu, Yin Jie, Yu Cheng-hua, Lu Zhi-yun, Liu Meng-kun, Ma Xiao-fei
    Food and Fermentation Industries. 2012, 38(01): 161-164. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.045
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    Research developed a polyamide absorption-HPLC method to determine 20 kinds of water-soluble pigments in beverages and candy.Samples were extracted with water,then absorbed-desorbed by polyamide,finally analyzed by Eclipse XDB C18 column(250 mm ×4.6 mm,5 μm).The mobile phase was: 0.02 mol/L ammonium acetate – methanol-acetonitrile;wavelength was 450nm,520 nm and 620nm,respectively.A good linear relationship was obtained from 2.00 μg/mL to 50.0 μg/mL.The R.S.D.of samples reproducibility(n=6) was 0.51%~7.8%;the recovery of the method was in the range of 71.4%~109.2%;the limit of detection was 0.03~0.3 mg/kg.The method was accurate and reproducible,it was suitable for analysis of several water-soluble pigments in beverages and candy simultaneously.
  • Tang Yuan-jiang, Wu Xiao-yan, Cao Wen-jing
    Food and Fermentation Industries. 2012, 38(01): 165-168. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.008
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    Providing reference for the component analysis and development of acid tea.the contents of water extract,polyphenols and free amino acid were determined according to the national standard method of food.Seven trace elements,such as Fe,Zn,Cr,Cu,Mn and Ni,were determined by flame atomic absorption spectrometry(FAAS) after wet digesting.The contents of moisture,water extracts,polyphenols,free amino acid,soluble sugar,chlorophyll and total flavonoids in acid tea were determined as 7.16%,49.13%,26.29%,0.88%,19.90%,0.054% and 6.56% respectively.The sequence of trace element content in acid tea was Mn,Cu,Cr,Fe,Zn from high to low.However,Ni was not found.The recovery rate of various experimental elements was 98.70%~101.80%,RSD is 0.04%~2.35%,with the accuracy and precision according to the requirements.Polyphenols and soluble sugar were higher;FAAS can be used in determination of metal element contents in acid tea.
  • Zhou An-fei, Yang Yan-li
    Food and Fermentation Industries. 2012, 38(01): 169-171. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.046
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    A method for the simultaneous determination of Pb,As,Cu,Cd in master batch by ICP-MS microwave digestion was studied.This method has simple operation,rapid analysis,highly accurate,reliable,and can be applied in simultaneous determination of heavy metal elements in master batch.Recoveries of the elements from the spiked master batch sample were in the range of 95.2%~101.8%,and the RSD was less than 3%.The results obtained agreed quite well with the certified values.
  • Ye Fu-lin, Gu Sai-qi, Liu Yuan, Wang Xi-chang
    Food and Fermentation Industries. 2012, 38(01): 172-177. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.009
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    In order to explore the effect of freeze-thaw cycles on tuna meat,physicochemical properties,such as thawing loss,a* value,textural properties-Hardness,Cohesiveness,Springiness and Chewines,volatile odor(Electronic nose),total viable count(TVC) were carried out.Yellowfin tuna(stored at-18℃,-60℃,respectively) was subjected to a total of 4 freeze-thaw cycles.The results showed that with the number of freeze-thaw cycles increased,thawing loss increased and the highest was at-60℃.a* value decreased significantly after 4 freeze-thaw cycles at-18℃,and showed no significant change at-60℃.Hardness,Springiness and Chewiness decreased significantly,while Cohesiveness remained unchanged.Electronic nose analysis showed that there was a significant difference in volatile odor of tuna stored at-60℃when compared to-18℃.TVC showed no significantly change,and it was below Industry Standard Limits.Therefore,the temperature should be kept stable during processing,transportation and storage of tuna.The best temperature is-60℃.
  • Zhang Qin-fa, Xu Xia, Zhi Ling-ling
    Food and Fermentation Industries. 2012, 38(01): 178-180. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.047
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    Fresh fish which was packed with packaging material having lower O2 permeability(material A) and higher O2 permeability(material D) under vacuum-packaging,custom packaging and CAP(Controlled Atmosphere Packaging)using mixture of O2 and CO2.Influence of packaging technics on quality of the packaged fish was studied.The results show that packaging technics had a remarkable influence on the quality of the packed fish.The higher content of O2 in package was,the steeper change of TVB-N value of fish packed was.The change rate of TVB-N value was 80%O2+20%CO2>custom package>50%O2+50%CO2>vacuum package when the fish is packed with material having lower O2 permeability while the change rate of TVB-N value was custom package>80%O2+20%CO2>vacuum package when the packing material had higher O2 permeability.The higher content of O2 in package was the steeper change rate of TBA value was.
  • Qiu Shu-bing, Zhang Yi-min, Luo Xin
    Food and Fermentation Industries. 2012, 38(01): 181-185. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.048
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    In order to study changes of the microbiology and meat quality of the vacuum-packaged beef and the correlation between their chemical index and microbiological index,we measured total viable count,lactic acid bacteria,Pseudomonas spp.,Enterobacteriaceae,Brochothrix thermosphacta,pH value,total volatile basic nitrogen(TVBN),water holding capacity,meat color of chilled beef and their correlations at 4℃ on the 0,7,13,21,28,and 38 days.The results showed that the change of the total viable count and the lactic acid bacteria were the same,that Pseudomonas spp.,Enterobacteriaceae,Brochothrix thermosphacta grew rapidly.The pH value decreased at first and then rose.The color increased at first then decreased little later.The TVBN value slowly rose in the early days and rapidly rose in the later days.The water holding capacity at 4℃ increased steadily.During the storage,there was a significant correlation between total viable counts and lactic acid bacteria(P<0.01) and there was also a correction between the total viable counts and TVBN value,a* value,b* value,C* ab and the water holding capacity(P<0.05),but there was little correction between total viable counts and pH value,L* value.Therefore,the meat quality changed because of the growth of the microbiology,and TVBN value,a* value,b* value,C* ab could be used as the indicator of meat spoilage.
  • Wang Zheng-yun, Li Zhi-fang, Shi Shuai
    Food and Fermentation Industries. 2012, 38(01): 186-189. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.049
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    In order to prolong the shelf life of chilled pork during cold storage,the preservation effects of different concentrations of tea polyphenols combined with chitosan on chilled pork were studied.The total bacteria count,TVB-N value,pH value,color and TBA value were used to evaluate the pork quality during cold storage.The results showed that the hair tail covered with 0.7% tea polyphenols combined with 1.0 % chitosan had the best quality,significantly better in quality than both uncoated one and the one only covered with chitosan.The shelf life of chilled pork treated with 0.7% tea polyphenols and 1.0 % chitosan was extended to 16 days.
  • Liang Xiao-lu, Chen Yi-lun
    Food and Fermentation Industries. 2012, 38(01): 190-194. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.010
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    With fresh yali pear as the raw material,different concentrations of calcium ascorbate solution to soak the fresh-cut Yali pear was performed to study the sensory and physical and chemical change on the storage at 4℃.The results showed that the calcium ascorbate treatment can inhibit the browning of fresh-cut Yali pear,reduce the degree of softening,delay the increase of membrane permeability and inhibit the activity of PPO,and maintain the appearance quality and nutritional value of the fresh-cut Yali pear.
  • Fu Lin, Cheng Shun-chang, Wei Bao-dong, Leng Jun-ying, Feng Xu-qiao
    Food and Fermentation Industries. 2012, 38(01): 195-198. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.011
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    Effect of Treatments with 1-MCP and its Structural Analogues on Post-harvest Storage of Tomato was investigated.The effect of three cyclopropene compounds were used as ethylene action inhibitors on postharvest storage and senescence of tomato fruits.Green mature tomato fruits were treated with 1-MCP(1-methylcyclopropene),or its structural analogue 1-PentCP(1-pentylcyclopropene) or 1-OCP(1-octylcyclopropene) and stored at ambient temperature.Physiological and biochemical analyses related to fruit ripening and senescence were conducted every three days during fruit storage.The results indicate that treatment with 1-MCP or its structural analogues inhibited the respiration rate and ethylene production rate of tomato fruits,restrained the decline of fruit firmness remarkably,delayed the raise of soluble solid contents.The activity of POD and CAT in after-ripening stage fruits were inhibited effectively as well.Therefore,the ripening and softening of tomato fruits were postponed by treatment with the compounds.However,the effect decreased when the side chain changed from methyl to pentyl and to octyl.
  • Ma Li-hua, Qin Wei-dong, Chen Xue-hong Tian Li
    Food and Fermentation Industries. 2012, 38(01): 199-204. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.050
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    In order to preserve the quality and anti-oxidative activity of garlic cloves and extend its shelf life,complex films treatment was studied.The effects of compound-coating on weight loss rate,amylose content,PODenzyme activity,MDA content and anti-oxidative activity during storage at 5℃ were investigated.The results showed that 1.5% chitosan+1.0% xanthan gum+0.8% sodium alginate reduced the weight loss rate and MDA content,inhibited PPO activities of garlic cloves,effectually prevented to amylase lose,and preserve anti-oxidative activity of garlic cloves during storage.
  • Yang Ming-jun, Wu Jing, Wang Yong-gang, Li Zhi-zhong
    Food and Fermentation Industries. 2012, 38(01): 205-208. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.015
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    To optimize the extraction processing of polysaccharide from Piteguo.Based on single-factor experiments and the principles of Box-Behnken central composite experimental design,response surface methodology(RSM) with 3 factors and 3 levels was used to explore the effect of each factor on extraction rate of polysaccharide.The optimal extraction processing conditions of polysaccharide were Ethanol concentration of 72%,water-material ratio of 15:1(mL/g) and extraction temperature of 83℃.Under the optimal extraction conditions,the extraction rate of polysaccharide was up to 7.49%.Conclusion: RSM can improve extraction rate of polysaccharide from Piteguo.
  • Meng Fan-bing, Li Yun-cheng, Zhang Shu-rong, Zeng Xiao-man, Zhong Geng, *
    Food and Fermentation Industries. 2012, 38(01): 209-213. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.016
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    This paper describes the optimization of extraction process of polysaccharides from Ganoderma lucidumspore by response surface analysis.The effects of three independent variables(namely,ratio of water to material,ultrasonic extraction time and microwave-assisted extraction time)on the yield of polysaccharides from Ganoderma lucidumspore were evaluated.Extraction ratio of water to material of 20:1,ultrasonic extraction time of 55 min,microwave-assisted extraction time of 20 min and were found optimum.Under these optimized conditions,the experimental value of Polysaccharides yield was 2.23%.
  • Jing Si-qun, Zhang Xiao-ming
    Food and Fermentation Industries. 2012, 38(01): 214-217. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.017
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    With yield and purity of crude polysaccharide as evaluation indexes,the optimal extraction process conditions of polysaccharides from Coreopsis basalis flowers by ultrasoound-assisted compound enzymolysis was studied.Effect of enzymatic hydrolysis and ultrasonic treatment on the structure of Coreopsis basalis flowers polysaccharides was evaluated through the near infrared spectral analysis,no enzyme and ultrasound-assisted treatment was used as control.The results showed that the optimal extraction process conditions were: compound enzyme 2.5%(cellulase 2%,acid protease 0.5%),temperature 55℃,pH 4.5,2.0 h.Under these conditions,the yield of crude polysaccharide was up to 9.83%,purity was 28.58%,and the purity was up to 64.57% after polyamide column purification.The result of near infrared spectral analysis showed that enzymatic hydrolysis and ultrasonic assisted extraction technology had no significant effect on the structure of Coreopsis basalis polysaccharides,but they can improve the extraction yield of polysaccharides.
  • Huang Jing, Hao Wen-fang
    Food and Fermentation Industries. 2012, 38(01): 218-223. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.018
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    Artemisia sacrorum Ledeb.was extracted by ultrasound and the processing was optimized.The influence of four extraction variables on extraction yields of total flavonoids was investigated,including ethanol concentration,the ratio of material to solvent,ultrasonic power and time.Based on the results of our investigations,orthogonal test design L9(34)was used to optimize the extraction yields.In addition,heat reflux and maceration were also employed to compare with ultrasonic extraction processes.The results indicated that the optimum ultrasonic extraction conditions were as follows: ethanol concentration 60%(v/v),the ratio of material to solvent 1∶ 20(w/v),ultrasonic power 350 W,time 60 min.Under the above conditions,the total ?avonoids yields could arrive 69.55 mg·g-1.Meanwhile,the optimized ultrasonic extraction processes was stable and feasible.In addition,it had several remarkable advantages,including time saving,high efficiency and easier-operation.
  • Tian Hui, Ruan Qi-ping, Lai En-yang, Ou Ping, Li Qiang
    Food and Fermentation Industries. 2012, 38(01): 224-229. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.019
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    To investigate the adsorption characteristics and purification process of the flavonoids from Kalimeris indica by macroreticular resin.The static capacity absorption,static capacity desorption,static adsorption dynamics(quasi-order kinetic equation of Lagergren),static adsorption isothermal(Langmuir and Freundich),dynamic absorption ratio and dynamic elution ratio of seven types of macroreticular resin were studied and compared in order to find the best one,and the adsorption and desorption performances of the best one were studied in detail.The type of D101 resin gave the best effect of separation with the sampling rate 3 BV /h and concentrations of 9.36 mg/mL in pH 4-5,and the concentration of elution was 75 % ethanol with eluting velocity 3 BV/h.The purity of total flavonoids in the product was up to 70%,and the purification process was feasible.
  • Mu Ting-ting, Han Ling
    Food and Fermentation Industries. 2012, 38(01): 230-232. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.020
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    With the tartary buckwheat bran as material,the effect of subcritical extraction time,extraction kettle temperature and system pressure on total flavonoids content were analyzed by combination of single factors experiment and orthogonal experiment.During the extraction of total flavonoids by subcriticaland ultrasonic,these indices affected the extracting efficiency in the following orders: extraction kettle temperature>subcritical extraction time>system pressure.The optimal extraction conditions for flavonoids from tartary buckwheat brans were extraction kettle temperature 20℃,subcritical extraction time 3h and system pressure0.8MPa.Under these optimum conditions,yield of total flavonoids reached 90.05%.
  • Zhang Jing, Diao Shu-pin
    Food and Fermentation Industries. 2012, 38(01): 233-237. https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.025
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    S.cumini fruit at eighty percent ripe stage was used as material for this research.The extraction of proanthocyanidins from the fruits was studied by butanol-hydrochloric acid technology.The yield was by measuring the absorbance of the product.According to single factors tests and orthogonal optimization,the results showed that the optimal conditions were: concentration of acetone were 60%(pH=3),the ratio of extraction solution to sample was 9,the extraction temperature was 60℃ and the extraction time was 2h.The proanthocyanidins yield was 91.52% after being extracted 2 times.The proanthocyanidins content in the S.cumini fruit at eight percent ripe stage was 5.96%,which was 133.04% times of fully ripen fruits.