Xue Xiao-feng, Zhao Jing, Chen Lan-zhen, Wu Li-ming, Zhou Jin-hui, Li Ren-yong, Li Yi
An ion chromatography method was developed for determination of sugars in bee pollen and royal jelly.Sugars were extracted with hot water and cleaned up by solid phase extraction after protein precipitation by acetonitrile.Sugars were separated on a Carbora PA10 column with NaOH/ NaOAc solution as mobile phase.The limit detection of glucose,fructose,sucrose and maltose were 0.12,0.14,0.21,0.33 mg/L,respectively.The relative standard deviations of bee pollen were 4.4%~6.3% and the recoveries are 93.5%~102.2%,the relative standard deviations of royal jelly were 6.7%~10.2% and the recoveries were 95.0%~103.5%.The method was applied for the analysis of 20 bee pollen samples and 20 royal jelly samples.The average concentrations of glucose,fructose,sucrose and maltose in bee pollen samples were 9.9~20.6%,14.2~26.9%,1.8%~8.3%,0.33%~1.4%,respectively.The average concentrations of glucose,fructose,sucrose and maltose in royal jelly samples were 6.2~8.3%,7.0~8.7%,0.38%~3.6%,0.27%~0.83%,respectively.The method is simple and suitable for a routine quality sugar control of bee pollen and royal jelly.
