25 May 2011, Volume 37 Issue 05
    

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    Food and Fermentation Industries
  • Liang Jiang, Pei Xin-rong, Wang Nan, Zhang Zhao-feng, Wang Jun-bo, Li Yong
    Food and Fermentation Industries. 2011, 37(05): 1-6. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.006
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    To observe the effect of long-term feeding marine collagen peptide on the incidence and development of the spontaneous tumor,Sprague-Dawley rats at the age of 4-week were divided into the control group and three experimental groups.Control rats were fed with standard rat diet and the MCP-treated rats were fed with 2.25%、4.5% and 9%(wt/wt) MCP throughout the lifespan.The results showed that the spontaneous tumor incidence of MCP-treated rats were obviously decreased compared with the control rats,with significance in the 9% MCP-treated female group and the 4.5% and 9% MCP-treated male groups;the long-term administration of MCP did not affect the palpable time and growth time of mammary gland tumor and the final volume of mammary gland tumor of the 2.25% and 4.5% MCP-treated groups were significantly decreased compared with the control group;the lifespan of tumor-bearing rats in the MCP-treated groups were prolonged,with significance in the 9% MCP-treated groups;compared to the control group,the incidence of death from tumors was decreased in MCP-treated groups in comparison with the control group of both sexes.In conclusion,the long-term administration of MCP might have some inhibitory effect on the incidence and the development of spontaneous tumor.
  • Zhao Wei, Yang Rui-jin, Zhang Wen-bin, Hua Xiao, Tang Ya-li, Tao Jing
    Food and Fermentation Industries. 2011, 37(05): 7-11. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.040
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    The effects of high pressure processing(HP) on microstructure,ingredients and protein denature of fresh oyster were evaluated in the present study.The muscle fiber structures disappeared and became more compact due to the protein denature induced by HP treatments.HP treatments had significant impact on the ingredients of oyster,characterized by the dissolving out of salt soluble protein and ash contents.HP treatment could activate some proteases and enhance the proteolysis in oyster,and increase the free amino acid.The higher HP pressure(600 MP) could induce severe protein denature.
  • Gu Long-jian, Yuan Bo-en, Zhao Qiang-zhong, Zhao Mou-ming
    Food and Fermentation Industries. 2011, 37(05): 12-16. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.041
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    The method of adjusting pH combining heat treatment was used to changing the property of Intermediate of Soybean Protein.The properties,such as solubility,emulsifying capacity at normal temperature,emulsifying capacity after freezing-unfreezing treatment.As a result,pH & heat treatment made obvious effect on the properties of Intermediate of Soybean Protein.Intermediate of Soybean Protein was heated by 90 ℃after adjusting pH to 10.0,the solubility increased by 40% at neutral situation.And the droplet size of emulsion was 2.1 μm,decreased by 3.7 μm compared with the control(pH=7.0,normal temperature).Intermediate of Soybean Protein was heated by 90 ℃after adjusting pH to 2.0,the droplet size of emulsion was 3.7μm,decreased by 2.1 μm compared with the control.The stability of emulsifying and the ability of anti-freezing were both improved.
  • Ji Tong, Wang Bing-bing, Xia Li-ming
    Food and Fermentation Industries. 2011, 37(05): 17-20. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.021
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    Cellobiase production was carried out by recombinant Pichia pastoris containing Aspergillus niger cellobiase gene,and the main fermentation parameters were optimized.The fermentation result in shaking flasks showed that the optimal concentrations of glycerol and corn steep powder were 2.0%(w/v) and 3.0%(w/v) respectively,and the optimal pH value was 6.0.Methanol as carbon source and inducer has obvious effect on cellobiase production by recombinant Pichia pastoris.The activity of cellobiase could reach 9.01 IU/mL after 8d fermentation by addition of 1.0%(v/v) methanol,0.2%(w/v) glycerol and 0.3%(w/v) corn steep powder every 24 hours.The result is valuable in improving the efficiency of cellulose hydrolysis by cellulase and promoting industrialization of ethanol production from cellulosic material.
  • Huang Jing, Shi Jian-ming, Huo Wen-ting, Xu Qing-yang, Xie Xi-xian, Ning Chen
    Food and Fermentation Industries. 2011, 37(05): 21-25. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.022
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    The effects of nitrogen sources on the fermentation of L-tryptophan were studied with L-tryptophan producing strain E.coli TRTH.The results showed that yeast extract and ammonium sulfate was the optimal organic and inorganic source by carrying out fed-batch fermentation of E.coli TRTH in 30-Liter fermentor.High L-tryptophan yield was obtained when 1.0 g/L yeast extract and 10 g/L ammonium sulfate were added to fermentation medium.By controlling the concentration of NH4+ less than 120 mmol/L,the biomass and production of L-tryptophan achieved 53.42 g/L and 32.6 g/L,which accessed high cell density cultivation.
  • Zhou Qing, Wei Chun, Ying Xiang-xian, Zhang Yin-jun, Wang Zhao
    Food and Fermentation Industries. 2011, 37(05): 26-31. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.023
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    Six γ-aminobutyric acid(GABA)-producing lactic acid bacteria(LAB) strains were screened from different kimchi samples.The strain A showed the highest GABA-producing ability of 1.261 g/L.Strain A was named as Lactobacillus plantarum WZ011 via 16S rDNA analysis.Fermentation medium was optimized using single factor test and orthogonal design methods.The optimal medium composition was glucose 13,yeast 5,monosodium glutamate 12,C8H11NO3·HCl 0.15,CH3COONa2,MgSO4·7H2O 0.02,MnSO4·4H2O 0.001,FeSO4·7H2O 0.001,NaCl 0.001,and water 1 000 mL.It was concluded from the curve of the fermentation kinetics that GABA fermentation process included two stages of cell growth and product formation.When nitrogen source was decreased and C8H11NO3·HCl was added,the utilization rate of monosodium glutamate rose to 99% and GABA production rate were increased more than twice.Under the optimum conditions,the highest GABA production reached 5.814 g/L,which was 79% higher than that of original fermentation condition.Furthermore,the duration of reaching the sationary phase of GABA production was decreased for 48h compared to the original fermentation conditions.
  • Chen Gan-lin, Lin Bo, Sun Jian, Li Yang-rui
    Food and Fermentation Industries. 2011, 37(05): 32-34. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.042
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    A semi-product of sugarcane shrub,an alcoholic drink,was brewed from sugarcane juice by submerged fermentation using fruit wine yeast at room temperature.The characteristic parameter variations of sugarcane juice in the process of alcohol fermentation were investigated to determine the action mechanism of fruit wine yeast.The results showed that the alcohol fermentation cycle of sugarcane juice with 0.15 to 0.30 g/L fruit wine yeast ranged from 14 to 20 days,the alcohol content was 4.5%,the acidity ranged from 1.8% to 2.4%,and the total sugar content decreased to 0.1%.The fermented juice was delightful,clearer and bright with yellowish-brown color.It exhibited full-bodied vinosity and soft taste.The present study has provided a reference for producing fruit drinks and vinegar drinks by the submerged fermentation of sugarcane juice.
  • Zhu Li-ping, Huang Hui-li
    Food and Fermentation Industries. 2011, 37(05): 35-39. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.024
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    A chitosanase-producing marine strain was isolated by our laboratory.Identification by rDNA-ITS sequence analysis,and it is Penicillium oxalicum.This is the first time that Penicillium oxalicum's the ability of producing chitosanase is reported.For the purpose of fermentation chitosanase in industrial,we studied oligotrophic medium components,such as source of carbon,nitrogen source,saltresistance,acidresistance and rotary speed.We explored different kinds of cheap medium for production of chitosanase.We found the soybean powder was the best,and the medium of soybean powder was as follow(g/L seawater): soybean powder 25 g,and the optimal enzyme activity was 8.5 U/mL.This medium was insufficient 5% of the cost of original.It provided direct theory and experiment foundation on industrialized production of chitosanase.
  • Wamg Bin, Jiang He-yuan, Zhang Jiang-yon, Wang Wei-wei, Wang Yan, Huang Yong-dong
    Food and Fermentation Industries. 2011, 37(05): 40-44. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.014
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    Continuous preparation of theaflavins by immobilized polyphenol enzyme in packed bed bioreactor were performed in this paper and The apparatus of continuous preparation system was designed and maded by ourself.In this study,we used D152(macroreticular weak acidic acrylic acid cation exchange resin) as a matrix to immobolize laccase extracted from Trametes spp.Then we utilized this bio-materials to transformat tea catechins into theaflavins consecutively in a packed bed reactor.Four mainly facts were considerated in this experiment as following: temperature,substrate flow ratio,oxygen flow rate,pH and subsrate concentration.As a results,we selected the temperature at 28℃,substrate flow ratio 2.0 mL/min,oxygen flow rate 55 mL/min,pH 5.7 and substrate concentration 10mg/ mL as a optimized condition to biosynthesize theaflavins at a highly efficient.This experiment conditions will give a reference for large scale producing theaflavins basically.
  • Wang Jian-zhong, Ke Li-xia
    Food and Fermentation Industries. 2011, 37(05): 45-49. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.007
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    Compared to the single-strain fermented of Pleurotus ostreatus,the crude protein content of the corn straw substrate was increased from 8.13% to 13.41% when it was fermented by mixed-strain of P.ostreatus and Candida utilis.Different concentrations bean dregs powder(2%,3%,4%),(NH4)2SO4(1%,2%,3%),CuSO4·5H2O(0.04,0.4,4 mmol/L) and MnSO4(0.001,0.01and 0.1 mmol/L) were added to the basic medium for the further increasing of the crude protein content and the activity of ligninolytic enzyme system.The results showed that: bean dregs powder and(NH4)2SO4 at the all experimental concentrations,and 0.01 mmol/L MnSO4 increased significantly the crude protein content of the corn straw substrate,and the increasing effects were best when the concentration of bean dregs powder and(NH4)2SO4 were 2% and 3% respectively;bean dregs powder,(NH4)2SO4 and MnSO4 at the all experimental concentrations increased significantly the activities of lactase(Lac) and manganese peroxidase(MnP),0.04,0.4,4 mmol/L CuSO4·5H2O and 0.4,4 mmol/L CuSO4·5H2O increased significantly the activities of Lac and MnP respectively.The activity of Lac was at its peak when the concentration of bean dregs powder,(NH4)2SO4,CuSO4·5H2O,MnSO4 were 2%,3%,0.4 mmol/L and 0.01 mmol/L respectively,and the activity of MnP was the highest when the concentration of bean dregs powder,(NH4)2SO4,CuSO4·5H2O,MnSO4 were 3%,3%,4 mmol/L and 0.01mmol/L respectively.The correlation between the activity of Lac and the crude protein content of the fermented corn straw substrate was probably positive.
  • Jing Wei-xin, He Ye-qin, Wan Qian, Wan Lan, Han Jian-shu, Du Xiao-wei Li Zhen-he, Li Xiao-yan, Fu Xue-dong, Li Xiao-min
    Food and Fermentation Industries. 2011, 37(05): 50-55. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.033
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    The 24 kinds of metal contents and varying trends were analyzed in Fen liquor from three kinds of aspects which contained one-year original Fen Liquor in three different containers(red pottery,black pottery and stainless steel containers).Specifically,one-year commodity Fen Liquor in two kinds of bottles(porcelain bottles and vitreous bottles),old liquor from one year to thirty years in red pottery containers.Meanwhile,the metal's effect on liquor quality was discussed intensively.The results indicated that,among the three kinds of containers,contents of useful elements for liquor quality were the highest in the red pottery,lower in the black ones,and the lowest the stainless steel cylinder.Red pottery was the best for the liquors' aging and ripe effects;black pottery was the most useful for long-term storage;stainless steel cylinder was suitable for being a turnover and blending container.The two kinds of bottles had specific effects on the contents of metal elements in one-year commodity Fen Liquor.Vitreous bottles were more favorable to speed liquor's aging and especially suitable for new liquor storage;potteries were more suitable for old liquor storage.In addition,it was proved that the variation of the metal-element contents in old liquor was in connection with storage periods,that is,with the increase in the storage period,the majority contents of the metal elements increased totally.5~10 years is the best time frame for long-term storage,3 years for short-term storage,and it was applicable to choose different storage containers in different storage stages in order to accelerate the aging process and gain the old liquor effects within short time period.
  • Shang Chun-liang, Wang Zhi, Chen Xiong, Wang Jin-hua, Huang Ya-nan
    Food and Fermentation Industries. 2011, 37(05): 56-60. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.012
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    Effects of soybean oil and alanine on rifamycin SV biosynthesis in 250 mL flask were studied in this paper.When 3 mL/L soybean oil and 0.8 g/L alanine was added into the medium at 72 h,rifamycin SV production was increased by 5.3% and 6.9% over the control,respectively.Response surface methodology was used to study the interaction between soybean oil and alanine.Result showed that their optimal amount was 2.57 mL/L and 0.89 g/L,respectively.Under the optimal conditions,rifamycin SV production reached 5179 U/mL that was 12.3% higher than control.The results of organic acids analysis demonstrated that utilization rate of a-ketoglutarate and citriate was 0.86 mg/(L·h) and 0.77 mg/(L·h),respectively,which was 115.7% and 23.3% higher than that of control in the fermentation process from 72 h to120 h.Meanwhile,succinate concentration reached 1.01 g/L that was 16.1% higher than the control.The result indicated that the conversion efficiency of a-ketoglutarate and citrate to succinate in the TCA cycle was greatly improved with the addition of oil and alanine,which led to enhanced synthesis of succinyl-CoA and methylmalony-CoA and the facilitation of rifamycin SV biosynthesis.
  • Zhang Yong-wei, Hu Hong, Liu Liang-wei, Chen Hong-ge
    Food and Fermentation Industries. 2011, 37(05): 61-65. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.020
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    The xylanase produced from Picha pastoris was modified with different chemical reagents.The results indicated that the thermostability of the xylanase modified with diamino-PEG was improved.The modification condition of the xylanase with diamino-PEG was optimized.The molar ration of xylanase/ diamino-PEG/ EDAC/NHS was 1/1000/100.The relative residual activity of modified xylanase by using this condition was 61.6% after incubated 10 min at 55℃,over 29.1% than unmodified xylanase.The analysis of the characteristics of enzymological assay of the modified xylanase found that only the pH stability and thermostability changed.
  • Wang Xiang-yang, Jin Fei, Shi Qing-hong, Li Gang College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 00 , China)
    Food and Fermentation Industries. 2011, 37(05): 66-69. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.034
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    Monosodium glutamate(MSG),disodium 5'-Inosinate(IMP)and guanosine 5'-monophosphate disodium salt(GMP)are main components that enhance flavour.I+G is composed of 50% IMP and 50% GMP.Study on processing stability of MSG and I+G has food production and processing significance.This experiment mainly used HPLC to measure the content change of monosodium glutamate and I+G which were treated with different sterilization temperature,sterilization time and acidity.The results showed that when sterilization temperature was 85℃,100℃,121℃ for 30 min,monosodium glutamate lost 3.16%,13.21%,30.10% respectively,and I+G lost 8.18%,12.91%,22.97% respectively.When sterilization time was 5min,15min,30minutes at 100℃,monosodium glutamate lost 1.43%,7.25%,8.63% respectively,and I+G lost 6.08%,8.78%,10.12% respectively.When acidity declined from pH 7 to pH 3(pH 7,pH 6,pH 5,pH 4,pH 3) at 100℃ for 15min,monosodium glutamate lost 2.2%,10.30%,22.4%,39.09%,52.50% respectively,and I+G lost 7.59%,9.30%,10.60%,12.89%,26.33% respectively.
  • Wang Ting-ting, Li Da-peng, Xu Xiao-yan, Xu Yan, Han Zhi-nan
    Food and Fermentation Industries. 2011, 37(05): 70-72. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.035
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    The inhibitory effect of Coptis chinensis and Forsythia suspensa on six kinds of common microorganisms causing food contamination was detected,and the formula of synergistic effect was optimized.The inhibitory ability of Coptis chinensis and Forsythia suspensa on E.coli,S.aureus,B.subtilis,M.luteus,H.anomala and Aspergillus niger was measured based on Inhibition zone diameter and MIC,and their combined antimicrobial activity was judged based on FIC.For M.luteus,the vitro synergistic effect of Coptis chinensis and Forsythia suspensa was both optimized by formula test.The combined inhibitory ability of Coptis chinensis and Forsythia suspensa on these six microorganisms resulted in either synergy or activity effect.The best ratio of Coptis chinensis and Forsythia suspensa which is 4.523∶ 5.477,shows the greatest in vitro synergy effect on M.Luteus.
  • Guo Guang-hui, Wang Jin-peng, Tang Tao, Xu Xue-ming, Jin Zheng-yu
    Food and Fermentation Industries. 2011, 37(05): 73-78. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.013
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    Plackett-Burman design was used to select reaction conditions for large ring cyclodextrin(LR-CD) preparation,and the significant factors were optimized by Box-Behnken design.Results showed that,enzyme,temperature and time were three significant factors,t-test showed that model of Box-Behnken design was significant(P≤0.05),and lack of fit was no significant(P≥0.05).The regression coefficient R2 was 0.9404,which was showed a good regression result.The predicted yield of LR-CD was 56.58%,and the predicted conditions were enzyme 91.34 U/g,temperature 71.7°C for 6.8 h.Validation test showed LR-CD yield of 55%,2.3 folds higher than initial yield.Identification results showed that the degree of polymerization was ranged from 20 to 40,the ratio of cyclic component was 47%.
  • Wang Xue-rong, Zhou Ling, Xie You-hui, Peng Xiang-wei
    Food and Fermentation Industries. 2011, 37(05): 79-82. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.036
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    Goose bone mud was used as raw material in order to provide substrate for preparing hot reaction meat flavor.Hydrolysate enriched amino acid was prepared through directional hydrolysis after pretreatment.Effects of pretreatment temperature and time on goose bone mud enzymatic hydrolysis were studied in the article.Enzymatic hydrolysis Conditions were optimized through orthogonal test.Free amino acids of goose bone mud hydrolysate were determined.The results showed that the best pretreatment temperature and time of goose bone mud were 80℃,40min.The best enzymatic hydrolysis condition was: trypsin 10 000 IU/g,temperature 45℃,pH 7.5,ratio of the material and extraction liquid 1∶ 30,5h.In the best enzymatic hydrolysis,degree of hydrolysis of goose bone mud was 63.09%,average peptide chain length 1.59%,Nitrogen rate of recovery 56.78%.Goose bone mud hydrolysate contained 16 free amino acids,7 essential amino acids,content of essential amino acids was 61.35%.
  • Zhang Xin-jun, Yue Hai-mei, Zhang Fu-jun, Fan Li-qing
    Food and Fermentation Industries. 2011, 37(05): 83-87. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.001
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    Traditional purification of chitinase from the fermentation supernatant had some weaknesses such as complicated steps,difficult in controlling and low recovery ratio.Then we took experimental exploration and find a simple,fast and convenient method in the process of chitinase purification.This method may also be used in Batch processing.Detailed method is: fermentation supernatant which containing chitinase was first mixed with colloidal chitin,after centrifugation,the chitinase and the colloidal chitin precipitated and separated with other components in the supernatant.Then the precipitated was washed by distilled water for several times,the precipitate was then resuspended in distilled water and then sterilized at 30 ℃for 72h.After the colloidal chitin was completely hydrolyzed,chitin enzyme was purified and Abstracted.This method requires simple,mild purification conditions,ensuring the purified enzyme maintain its biological activity.SDS-PAGE and colorimetry detecting methods indicated that the recovery rate of chitinase using this method can reach 88.53%.
  • Liu Li, Chi Yu-jie, Xia Ning, Mao Wen-ying, Zhang Yan-yan, Yao Wan-ying, Sun Bo
    Food and Fermentation Industries. 2011, 37(05): 88-91. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.037
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    Microwave technology is applied in sterilization of soybean paste on three main parameters as power,time and net weight.The effects of these parameters are compared by colony forming unit to obtain the optimum microwave sterilization process.Sensory evaluation and color difference of the soybean paste that is microwave sterilized and pasteurized are also tested.The evaluation indicated that three parameters,power,time and net weight all have obvious influence on colony forming unit in the soybean paste.Technical parameters for optimization are microwave power of 3400 W,microwave time of 120 seconds and net weight of 100 gram per package.After the process,colony forming unit of sterilized samples decreases by 99.9%,Coli form bacteria is less than 3.0 MPN/g and has good quality according to the Hygienic standard for soybean paste in China.Change in quality of the soybean paste,especially the color,is even less noticeable after pasteurization.
  • Guo Meng-meng, Ren Ling-ling, Du Jin-hua, Xiao Lin, Qin Shu-lin, Wang Cong
    Food and Fermentation Industries. 2011, 37(05): 92-96. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.038
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    Xylose was added into flour as a substitute of sucrose in the direct fermentation method to make the bread.Rheological properties of the dough before and after adding xylose were determined with a Farinograph and a Extensograph.The study showed that the higher the xylose content,the lower the farinograph quality number(FQN),the shorter the stability and breakdown time,and the higher the softening and common difference index dough.Xylose also can cut down the dough water absorption,but had no effect on the dough developing time.The dough tensile energy,tensile resistance and extensile rate were decreased with the xylose dosage up,but the extension was increased.The correlation coefficients between xylose dosage and the indexes of tensile properities were decreased with the fermentating time extending,which implied that the effect of xylose on tensile prosperities was decreased.It was found that adding 1.5% of sucrose into the bread formula was enough for yeast growing.Xylose had little effect on the bread regular scale,but decrease the loaf volume,the specific volume and the sensory evaluation.The bread firmness reached its minimum value when xylose addition was 3.0%.The loaf volume,the specific volume and the sensory evaluation was reduced dramatically with xylose above 3.0%.The water-holding capacity(WHC) and the oxidation resistance of the bread were enhanced by xylose,while the acidity and acid value had little change.
  • Fu Cheng-cheng, Guo Yu-rong, Dong Shou-li, Li Jin-yun, Zheng Zhan-wei, Chi Xia-wei
    Food and Fermentation Industries. 2011, 37(05): 97-100. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.043
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    China's annually apple wet pomace is about 1million tons,which is rich in dietary fiber.In order to utilize the apple pomace,the paper studied to use it as a food additive in dietary fiber bread.The flour blended with 0%,5%,10% and 15% apple pomace powder to make apple dietary fiber bread.Through sensory analysis,specific volume,moisture retention and texture characteristic analysis,the main factors affected apple pomace dietary fiber bread quality were analyzed.The optimum formulations were determined.The results showed that with the increasing of apple pomace powder,the specific gravity and the hardness of the bread decreased,the capacity of water-holding and the springiness of the bread increased.The bread quality was the best with 5% apple pomace powder.
  • Xu Wei, Shi Hai-ying
    Food and Fermentation Industries. 2011, 37(05): 101-104. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.025
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    The fermentation conditions of producing lipase by Aureobasidium pullulans were studied and optimized.Methods: Temperature,initial medium pH and amount of inoculation were investigated by central composite design after the preliminary experiment was finished.Results: Temperature,initial medium pH and amount of inoculation were the significant effective factors on lipase activity in crude enzyme liquid.There was no interactive influence on lipase activity among temperature,initial medium pH and amount of inoculation.The regression model equation was obtained(R2= 0.9556).The optimum value of temperature,initial medium pH and amount of inoculation were 26.02℃,5.87 and 6.38% especially.Conclusion: The relationship could be explained by the regression model equation between lipase action and the temperature,initial medium pH and amount of inoculation.It could predicate and guide better the process of producing lipase by Aureobasidium pullulans.
  • Hu Qi-jie, Qi Min, Yao Zhao-min
    Food and Fermentation Industries. 2011, 37(05): 105-109. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.026
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    D-Mannitol production from fructose by lactic acid bacteria(LAB,Lactobacillus plantarum HSC 235) and propionate acid bacteria(PAB,Propionibacterium freudenreichii HZP-35) was investigated.Fructose can be converted into mannitol and sorbitol.Three kinds of reaction systems of growing cells,resting cells,and free cell extract were compared in mannitol formation using fructose as substrate.The results showed that the growing cells reaction process gave out a higher mannitol yield at 38 g/L,the Yp/s at 27%,while the last two reaction processes had a similar low mannitol yield.This suggested that mannitol formation was coincided with the cell growth of LAB and PAB.Initial fructose concentration hadn't appear effect on mannitol formation between 50-150 g/L where the mannitol Yp/s was at 27%,but high up to 200 g/L fructose had a inhibition on the conversion process.Although LAB and PAB can grow together as in many food,but the mixed cultivation of the two bacteria would not produce more mannitol compared with any single pure strain fermentation.In the ability of mannitol production,LAB showed a high level to PAB.
  • Wu Hai-bin, Liu Zun-ying, Zeng Ming-yong, Zhao Yuan-hui, Dong Shi-yuan
    Food and Fermentation Industries. 2011, 37(05): 110-114. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.044
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    The peptides was obtained by submerged fermentation of cod fish skin after which was cracked with Aspergillus oryzae utilized,The optimization was started from 8 aspects-the carbon and nitrogen ratio,liquid volume,substrate concentration,rotation speed,carbon resource,surfactants,different initial pH,inoculation rate,after which the most important factors was chosen-carbon and nitrogen ratio,inoculum,substrate concentration to do the response surface experiment.The optimum fermentation conditions were as follows: 0.29 ratio of carbon and nitrogen,7.0%substrate concentration,inoculum size 4×107,then the ratio of hydrolysis could come up to 36%,the molecular weight below 1000 took up about 71.5% of fementation peptide.This paper also monitored the activity of antioxidation as well as antihypertense of the peptides,which proved that the IC50 of DPPH could be 1.3 mg/mL,The IC50 of anti-ACE of peptides was 0.88 mg/mL,the peptide of this paper was in low molecular weight with high activity of antioxidation and antihypertense.
  • Yi Xing, Zhong Qiu-chen, Jiao Shuang, Zhang Jian-ping, Xiao Xiao-nian
    Food and Fermentation Industries. 2011, 37(05): 115-117. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.015
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    The sequential extractions using petroleum ether,ethyl acetate and n-butanol were applied to water extract of Alisma orientale.The aqueous phase was added on a column of macroporous resin and was washed with distilled water and 95%ethanol,successively.In addition,the inhibitory activity against α-glucosidase of each fraction was determined and the inhibitory kinetics of the fraction with the strongest inhibitory activities was investigated.The results showed that the fraction with the strongest inhibitory activities was n-butanol fraction,a kind of non-competition inhibitor with inhibitory ratio of 92.30%.
  • Cheng Ni, Gao Hui, Wang Bini, Deng Jian-jun, Liu Yuan-yuan, Cao Wei
    Food and Fermentation Industries. 2011, 37(05): 118-122. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.008
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    Antioxidant activities of the pine pollen extracts was studied.Phenolics were extracted from the pine pollen using water and 80% ethanol as solvents.The extracts are analyzed by HPLC-ECD.And the scavenging effects of extracts on hydroxyl radical-mediated 2-deoxyribose degradation were studied.The results revealed that protocatechuic acid,caffeic acid,p-coumaric acid,ferulic acid and galangin could be identified and quantified in both extracts.Rutin,quercetin and kaempferol were identified and quantified in ethanol extracts.The total phenolic content of ethanol extracts was significantly higher than water extracts(P<0.05).Reducing power and chelating activity of water extracts were significantly higher than ethanol extracts(P<0.05).The both extracts performed better in the nonsite-specific assay than in the site-assay(P<0.05).It was also found that pine pollen extracts had higher scavenging activity than iron chelation ability for hydroxyl radicals.This may due to the high level of phenolic compounds in the pine pollen.
  • Zhang Xiao-li, Zhao Chun-yan, Wu Xing-zhuang, Zhang Hua, Lu Ming, Wang Xiao-he
    Food and Fermentation Industries. 2011, 37(05): 123-126. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.027
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    peaches was fermented by lactobacillus to make the peach block.then the peach block was cooked by sugar,soaked at normal temperature and dried.The result showed that the technique of lactobacillus fermented peaches was: peach block boiling in 45% sugar for 10 min until the peaches totally cooked,the soak in sugar for 24 h at normal temperature,then put the peach block into the oven and bake at 65℃,20 h.The preserved peaches taste sour and sweet,and not only have the peach flavor,but also the peculiar flavor that lactobacillus fermentation added.
  • Pang Xue-li, Hu Xiao-song, Liao Xiao-jun, Zhang Ming-wei, Wu Ji-hong
    Food and Fermentation Industries. 2011, 37(05): 127-131,138. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.028
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    The characteristic aroma of thermo-sensitive vegetables and fruits is easily deteriorated during traditional thermal processing.Based on comprehensive comparison between thermo-sensitive and nonthermo-sensitive representative fruits and vegetables,this article summarized the compositional features of thermo-sensitive fruits and vegetables,characteristic aroma,pointed out the reason for their aroma deterioration during thermal processing;Taking Hami melon as representative,,this paper furtherly overviewed the up-to-date technologies suitable for aroma analysis of temperature-sensitive fruits and vegetables,compared and analyzed the influence of different non-thermo processing technologies on aroma profile,providing theoretical foundation for development of analysis method and aroma profile quality control for aroma characteristic of thermo-sensitive fruits and vegetables.
  • Zhang Tan, Tan Tian-wei, Ling Peixue
    Food and Fermentation Industries. 2011, 37(05): 132-138. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.029
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    The article introduced the characteristics of microcapsule wall materials in food industry.Its detecting method and the advantages and disadvantages of the common wall materials in practical applications are also discussed.Especially,the latest research progress of carbohydrates,natural plant gum,proteins wall materials and novel wall materials were summarized.
  • Ying Li-ya, Su Ping
    Food and Fermentation Industries. 2011, 37(05): 142-145. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.017
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    Essential oils from natural plants contain a variety of bioactive ingredients,which are widely used in medicine,food,tobacco and cosmetic industries.Subcritical water extraction(SWE) technology,a new type of green extraction technology,is getting more and more attention.Its application in the extraction of plant essential oil is of great importance for improving the quality of the essential oils.This paper introduces the principle of the research progress,the recent application and research on the plant essential oil extraction of SWE.Finally,its potential application and development prospect in this field are also explored.
  • Wang Li, Xu Zhen-bo, Zhao Xi-hong, Zhong Qing-ping
    Food and Fermentation Industries. 2011, 37(05): 146-150. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.003
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    The specificity and sensitivity of a loop-mediated isothermal amplification(LAMP) method was developed and evaluated for rapid detection of the food-borne Escherichia coli O157 strains.Six primers,including outer primers,inner primers and loop primers,were specially designed for recognizing eight distinct sequences on three targets,which were rfbE,stx1 and stx2.The detection limits were found to be 100,100 and 10 fg DNA/tube for rfbE,stx1 and stx2,respectively.Application of LAMP assays were performed on 417 food-borne E.coli strains,the sensitivity of LAMP assays for the rfbE,stx1 and stx2 was 100%,95.3% and 96.3%,and the negative predictive value(NPV) was 100%,96.7% and 97.1% respectively;with a 100% specificity and positive predictive value(PPV) for all three targets.The results showed that the LAMP assay has proven high specificity,sensitivity and easy operation features in the detection of Escherichia coli O157.It also implied that the assay owned good application prospect in food safety detection.
  • Mei Hui, Dai Jun, Liu Wen-wei, Song Peng-fei, Wu Jing, Zhao Zhi-jun
    Food and Fermentation Industries. 2011, 37(05): 151-156. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.004
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    An high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS) method was established for the determination of 24 intracellular metabolites in E.coli.After quenching,washing and extraction,the samples were centrifuged to remove impurities,and then separated on a Waters Atlantis HILIC silica column.The mobile phases were acetonitrile and water(containing 10 mmol/L ammonium acetate) at a flow rate of 250 μL/min.The electrospray was operated in the negative ionization mode and the 24 metabolites were identified by multiple reaction monitoring(MRM) mode.The method of matrix-matched standard solution was adopted as the quantitative method.The calibration curves showed good linearity within the concentrations of 0.10~100 μmol/L with the correlation coefficients r >0.99.The limits of detection of the 24 metabolites were 0.05~0.34 μmol/L.The recoveries of the 24 metabolites were 83.7%~108.1% at the 3 spiked levels.The relative standard deviations were less than 10%.This method is simple,sensitive and accurate in the determination of 24 metabolites.
  • Lin Song, He Yan-ling, Wang Li-zhe, Zhao Yu, Yuan Fei, Yang Hai-rong, Chen Ying Chinese Academy of Inspection and Quarantine, Beijing , China)
    Food and Fermentation Industries. 2011, 37(05): 157-159,165. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.039
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    To establish rapid assays for individual detection of Staphylococal Enterotoxin A,B and C(SEA,SEB,SEC) in raw milk samples,Lateral Flow Immunoassay test strips were studied.The models of nitrocellulosemembrane and the pretreatment methods of raw milk were chosen and optimized,and the membrane of HF09002 and the method of 2 times dilution showed the best result for the field detection of raw milk samples.The Sensitivties of Staphylococcal Enterotoxin A,B and C test strips were 10ng/ mL,4ng/ mL and 8ng/ mL,and there is no observe difference objected that compared with 3M(tm) Tecra(tm) Staph Enterotoxin ID Test method.
  • Lu Jiang, Xu Yang, Chu Jin-shen, Xie Lin
    Food and Fermentation Industries. 2011, 37(05): 160-165. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.030
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    A sensitive indirect competitive chemiluminescence enzyme immunoassay(CLEIA) for zearalenone in corn has been developed.The optimized CLEIA gave a limit detection of 0.09 ng/mL and a detection range of 0.104-1.69 ng/mL,with an IC50 of 0.438 1 ng/mL and it was about 3 times more sensitive than that of colorimetric ELISA.It has been validated on cereals samples in terms of precision(intra-and interassay coefficient variations of less than 9.1﹪ and 14.7﹪,respectively) and accuracy(mean recovery 79.6﹪~97.6﹪).These assay performances indicated that CLEIA was capable of being applied for the specific detection and routine monitoring of ZEN in corn samples.
  • Chen Liang, Xin Xiulan, Wang Yanhui, Yuan Qipeng
    Food and Fermentation Industries. 2011, 37(05): 166-169. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.018
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    To establish a method to determine anthocyanin content and composition in red raspberry by HPLC-DAD-ESI-MS.Methods: The analysis was carried on Zorbax SB-C18 column(250 mm×4.6 mm,5 μm).The mobile phase was methanol-5% formic acid-water at the flow-rate of 1.0 mL/min,the detection wavelength was 520 nm and the scan wavelength was from 200 nm to 600 nm.Results and conclusion: The content of gross anthocyanins was 105.69 mg/100 g by HPLC calculated as cyanidin 3-glucoside equivalent.Four anthocyanins in red raspberry were identified by HPLC-DAD-ESI-MS,which are cyanidin 3-sophroside,cyanidin 3-sophroside 5-rhamnoside,cyanidin 3-glucoside and cyanidin 3-rutinoside.Their relative content was 22.05%,13.83%,33.74% and 30.38%,respectively.
  • Zhu Wei-ping, Wei Qing-yi, Ning Zheng-xiang
    Food and Fermentation Industries. 2011, 37(05): 170-174. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.019
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    GC-MS method was used to detect the variation of flavor and aroma substances from different milk flavor samples.There are 18,22,30,15,30,14 kinds of components were identified in artificial milk flavor A,artificial milk flavor B,artificial milk flavor C,artificial milk flavor D,ferment milk flavor E and self-made hydrolysis milk F.The components were divided into acids,lactones,esters,aromatics,ketones,alcohols and hydrocarbons.The analysis of different milk flavors showed that the major compounds in each sample were different.Acids,lactones,esters are the main compounds in artificial milk flavors,and the proportion were varied.Foreign ferment milk flavor E contains aromatics besides acids,lactones and esters.Acids were the key odorant compounds in self-made hydrolysis milk F.The balance among the varieties of compounds can improve the flavors quality in certain degree.
  • Chen Hui, Zhang Yan-qing, Zhang Han, Tong Jun-mao
    Food and Fermentation Industries. 2011, 37(05): 175-180. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.031
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    The individual and group thresholds of nine organic acids were measured in control and degassed beer using the ASTM method 679 protocol.The results show that the group threshold of malic acid in control beer had the highest value in the 9 organic acids,followed by fumaric acid,lactic acid,citric acid,pyruvic acid,succinic acid,acetic acid,oxalic acid and tartaric acid in rank.The panelists have relative small differences in taste of malic acid,succinic acid,fumaric acid,oxalic acid and citric acid in the control beer,and the great discrepancy in mouth-feel of tartaric acid,acetic acid,pyruvic acid and lactic acid.The group thresholds of nine organic acids in degassed beer followed by pyruvic acid,lactic acid,acetic acid,fumaric acid,succinic acid,malic acid,tartaric acid,oxalic acid and citric acid in rank.The panelists have relative small differences in taste of citric acid,malic acid and lactic acid,and the great discrepancy in mouth-feel of pyruvic acid,oxalic acid,tartaric acid,fumaric acid,succinic acid and acetic acid.Besides,we have found significant difference in the group thresholds of nine organic acids between degassed and control beers.Triprotic acid has more significant difference than other acids,and monoprotic acid was the smallest among these acids,while binary acid lie in the middle.
  • Wang Dan, Guo Meng-meng, Du Jin-hua
    Food and Fermentation Industries. 2011, 37(05): 181-185. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.032
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    Taking ethylferulate as substrate,the optimal enzymatic hydrolysis conditions of feruloyl esterase were confirmed as hydrolysis time 100min,the volume ratio of enzymes and substrate 0.75 mL/mL,the pH value of extract 6.2.The study on the characteristic of feruloyl esterase exhibited that the optimal temperature was 60℃,and it had a better thermal stability between 30~35℃,remaining over 80% of enzyme activity after insulation;the optimal pH value was 5.0,and the destruction was relatively weaker,remaining over 80% of enzyme activity after insulation;The activity was inhibited by Cu2+,Zn2+,and strongly activated by EDTA.The activity of feruloyl esterase was determined for five kinds of wheats and malts,and it was found that all of the activity of the enzyme was raised after germination in which the increase of Yan 2415,Lu Mai 21 and Zheng Mai 004 was maximum.
  • Cui Heng-wei, Li Bo, Du Qi-zhen
    Food and Fermentation Industries. 2011, 37(05): 186-188. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.005
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    The present study describes the separation of syringaresinol from the legume of Mucuna sempcrvircns by high-speed countercurrent chromatography(HSCCC) with a solvent system of methyl tert-butyl ether-methanol-water(2∶ 1∶ 1,v/v/v).Crude syringaresinol of 1.28 g with a purity of 89.5% was obtained from a separation of 5 g crude extracts.The crude syringaresinol was re-crystallized to more than 98% purity of syringaresinol.The chemical structure of the substance was identified by ESI-MS,1H NMR and 13C NMR.The syringaresinol content in the legume samples collected in different districts in Hangzhou were all more than 3.0 g/kg.This shows that the legume is an excellent resource for preparation of natural syringaresino which can be used for research and development of health food and medicine.
  • Gao Yun-tao, Jiang Jin-chao, Zhang Bao-ton
    Food and Fermentation Industries. 2011, 37(05): 189-193. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.016
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    Ultrasound-assisted propyl alcohol ammonium sulfate aqueous two-phase extraction of polyphenols from Phyllanthus urinaria L.was investigated.The best condition for the single factor experiment was: propyl-alcohol concentration 60.0%,liquid/solid ratio B 25.0,extraction time C 30min.A Response surface methodology,with a five levels,four variables central composite designs was applied based on the single factor optimization experiment.The best condition was: propyl-alcohol concentration 53.65%,liquid/solid ratio 23.97,extraction time 28.19 min,and ammonium sulfate concentration 0.40 g/ml.An experimental extraction yield of 8.72 %(n=5)was achieved under the optimal extraction which was very close to the optimal prediction extraction yield(8.67 %),and was higher than that of single factor optimization experiment(8.61 %,n=5).
  • Yin Xiu-lian, You Qing-hong, Jiang Zhong-hai, Zheng Shang-yong
    Food and Fermentation Industries. 2011, 37(05): 194-198. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.009
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    The antioxidant activity of different polar solvents extracts and the effective substance of Tricholoma matsutake were studied.Dried Tricholoma matsutake Sing was sequentially extracted with petroleum ether,chloroform,ethanol and water,the ethanol extract was fractionated into ethyl acetate and n-butyl alcohol-soluble fractions.Using BHT,Vc as reference,the antioxidant activity of the extracts was evaluated by DPPH radical scavenging and pyrogallol auto-oxidation assay,then quantity of the total polysaccharide,total flavonoids,total phenols of different fraction were analyzed.Results show that all the extracts exhibited some antioxidant abilities in a dose-dependent manner.The ethyl acetate fraction and the water fraction showed the highest antioxidant activity,and the antioxidant activity has correlation with the content of total polysaccharide,total flavonoids,total phenols.
  • Sun Yan-yu, Zhou Guang-hong, Xu Xing-lian
    Food and Fermentation Industries. 2011, 37(05): 199-203. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.045
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    This study was designed to identify the critical control point during slaughter by exploring the microbial diversity in the produce of broiler using polymerase chain reaction ·denaturing gradient gel elec-trophoresis(PCR-DGGE).In stage of slaughter,the contamination microorganism was sampled from broiler carcasses surface,the surface of workers' hand,the water in the pre-cooling pool,the air and broiler longissimus muscles of postmortem broiler carcasses,which was vacuum packaging at 4℃ for 7 day.The results indicated that the processing of different treatment in the process of broiler slaughter will have different effects on microbial contamination of carcass surface,the type and number of microorganisms have increased after the broiler carcass to the visceral.However,the type and number of microorganisms have reduced after pre-cooling,microorganisms in the air increase in production.These operating points are also into areas of cross-contamination,in particular,the surface of workers'hand is the main reason causing cross-contamination.
  • Xie Nan, He Wei-jia, Chen Xi
    Food and Fermentation Industries. 2011, 37(05): 204-208. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.046
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    The protective culture(HOLDBACTM-YM-C) was applied in the making of a new yoghurt brand that features inhibitory function for yeast and mould growth control.Using the orthogonal test on the inoculations of protective culture and yoghurt starter culture,fermentation temperature and sugar adding amounts,we have determined the best fermentation condition.The best fermentation parameter combinations,based on the evaluation of fermentation parameters,change of pH value,number of viable lactic acid bacteria,and texture characters during the storage,were: protective culture 10DCU/100L,yoghurt starter culture 7.5DCU/100L,fermentation temperature 37℃,sugar adding 8%,and yoghurt had very good antimicrobial property.
  • Zhang Chun-hong, Sun Jing, Xu Jiao, Liu Chang-jiang
    Food and Fermentation Industries. 2011, 37(05): 209-212. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.047
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    The optimum production conditions of stirred actinidia arguta fruit yogurt adding immobilized TG was studied.The results showed that the best indicators of quality and sensory are the inoculation concentration 3.5%,fermentation temperature 42℃,fermentation time 3.5 h,the immobilized enzyme addition 30 g/L and fruit 35 g/L.Under the said condition,yogurt syneres,apparent viscosity and storage viscosity is up 139.2%,32.9% and 42.9%,water holding capacity is down 35.8%.Compared to nature enzyme,the immobilized TG has relatively high stability,can be reused for more than 8 times and maintain more than 80% enzyme activities.
  • Wang Kaituo, Zheng Yonghua
    Food and Fermentation Industries. 2011, 37(05): 213-217. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.010
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    Response surface methodology(RSM) based on a two factors-three level central composite design was applied to optimize hot air treatments.The preserving quality parameters includes decay incidence,firmness,Vc content and TSS/TA in freshly harvested Chinese bayberry fruit were measured after store at 1 °C for 7 days followed by 20 °C for 1 day.According to the response surface analysis,the lowest incidence of decay(18.81 %) and highest values of firmness(3.31 N),Vc content(5.25 mg/kg(FW)) and TSS/TA(16.42) were achieved by a hot air treatment at the temperature of 48.5 ℃ and exposure time of 173 min.The data obtained provided the foundation for application of the hot air treatment in quality maintenance of Chinese bayberry fruit.
  • Zhang Bing-yun, Wang Le, Wang Yu-li, Wang Yong-gang Zheng Wei-bin, Wang Shou-yu
    Food and Fermentation Industries. 2011, 37(05): 218-220. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.048
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    In this paper,Malonate was used as vegetable fresh keeping agent for the first time,fresh leek sprouts was the raw material.The leek sprouts was treated with three different concentrations,100 mg/L、300 mg/L、500 mg/L.The changes of physiological and biochemical index during the shelf life were studied.The result showed that leek sprouts was treated with 300 mg/L concentration was kep more than 7 days.Moreover,the leek sprouts still maintain good shape and high nutritional value.
  • Bai Jie, Ma Yong-kun, Zhang Long, Yan Rui, Ma Shan-li, Wei Ben-xi
    Food and Fermentation Industries. 2011, 37(05): 221-225. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.049
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    The effects of high pressure processing(HPP) at 300~600 MPa for 15 min on organic acids and VC in blackberry(Rubus Hull.) juice were detected by using reversed-phase high performance liquid chromatograp method and 2,4-Dinitrophenylhydrazine colorimetry method respectively.Their synergistic antioxidant capacities were also evaluated.In blackberry juice,the dominant acid was L-malic acid,which constituted 87.98% of total acids.While α-ketoglutaric acid,acetic acid,citric acid,and butanedioic acid were detected in low amounts.After application of pressure,L-malic acid and butanedioic acid were significantly changed(P<0.05).However,no significant changes in other organic acids(P>0.05).L-malic acid in juice decreased by 12.2% after 300 MPa processing for 15 min,while increased by 8.53%,6.08% and 22.35% respectively at 400~600 MPa processing for 15min.Compared with the control juice,the contents of VC were well retained and ranged from 90.10% to 97.92% at 300~600 MPa.L-malic acid enhanced the scavenging activity of VC on DPPH· with its concentration increasing,which indicated that L-malic acid synergistically enhanced the antioxidant capacity of VC.
  • Wang Ping, Chen Yi-lun
    Food and Fermentation Industries. 2011, 37(05): 226-229. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.011
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    Using Mopan,Niuxin and Jingmian persimmons as raw materials,the changing of oxidative stability on processing of different varieties of persimmon juice was studied by the methods of DPPH scavenging,·OH scavenging and O2· scavenging,and compare the changes of the total phenol,total flavone,Vitamin C,carotenoid content during processing.The results showed that: persimmon juice of different varieties all had certain antioxidant capacity,and all excelled cider.But the antioxidant capacity of Niuxin persimmon juice as always the strongest,and the antioxidant capacity was more stationary during processing.The antioxidant capacity and the content of total phenol,total flavone,Vitamin C,carotenoid of persimmon juice were reducing trend.The content of total sugar,total phenol and total flavone in Niuxin persimmon juice was the highest,and had more nutritional value and functional characteristics
  • Wang Yang, Zhou Guo-ping
    Food and Fermentation Industries. 2011, 37(05): 230-233. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.050
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    One lot of tea drinks were found deteriorated,with pH decrease,gas production,swell can,visible deposition and discoloration.Over 104 CFU/mL bacteria were detected in 3 samples.Four main bacteria were isolated and identified by 16S rDNA sequence blast as Pseudomonas sp.,Enterobacter sp.,Bacillus sp.,and Brevibacillus sp.A few fungi were also detected in all 3 samples with amount of 101~102 CFU/mL.They were identified primarily as Alternaria spp.,Cladosporium spp.,Aspergillus spp.,and Penicillium spp.In this case,although fungi were not the main putrefactive organisms,but its unique flora were just the same as those of air,indicating that air was probably the pollutant source.And the bacterial flora also supported this deduction.In practice,the pollution was solved by replaced the air filter to confirm the conclusion.
  • Jiang Pei, Kang Jian, Wang Ling, Ma Fang-fang, Wu Tong, Zhang Li-xia, Xie Cui-pin, Liu Lu-ping
    Food and Fermentation Industries. 2011, 37(05): 234-238. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.051
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    Extraction of oil from bu er jin seabuchthorn pulp and its process technology of beverages were investigated.Through the single factor experiment and the orthogonal experiment,the optimal extraction conditions were determined: the amount of Pectinase was 0.50%,Pepsin was 0.08%;temperature was 55℃;and the extracting time was 6 h.Under these conditions,the oil extraction rate is up to 1.9875%.And the residue was used to product clear juice or turbid juice.The optimal formula of turbid juice were as follows: seabuckthorn puree 30%,sugar 14%,CMC 0.3%,Xanthan gum 0.03%.And the optimal ingredient of the clear juice were: seabuckthorn puree 60mL,sugar 14%,water 150mL,Vc 0.2%.
  • Zhou Zhi-de, Li Gui-yin, Huang Yan-ping
    Food and Fermentation Industries. 2011, 37(05): 239-242. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.052
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    A process of Aloe and honey compound beverage was studied.The main ingredients are aloe and honey.Citric acid,sugar,sodium iso-VC are used as food additives.Through a scientific study,a health benefits natural beverage was produced.In this paper,the production process and formulation was studied and a reasonable processing parameters were investigated.Orthogonal experimental results showed that the best formula was a combination of 20% of aloe vera juice,12% of honey,3% of sucrose,0.15% of citric acid.The best Stabilizers formula were 0.20% CMC with 0.10% xanthan gum.