25 July 2014, Volume 40 Issue 07
    

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    Food and Fermentation Industries
  • XU Jun, YONG Xiao-yu, FEI Wen-bin, ZHOU Jun, WANG Shu-ya, CHEN Yi-lu, ZHENG Tao
    Food and Fermentation Industries. 2014, 40(07): 1-5. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.012
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    A quantitative screening method for yeast with high activity was established based on the TTC( Triphenyl- tetrazolium chloride) staining method and 96-well plate. The optimal test conditions were 85%( V /V) dimethyl sulfoxide,0. 4% TTC,pH 8,and testing wave length 486nm. This assay was performed in 96-well microtitre plate.Twenty seven strains were cultured in the 96-well microtitre plate with 0%,10%,and 14%( V /V) ethanol. The TTF in cell was detected by microplate reader. Strains with higher TTF absorption values had higher ethanol producing ability. The research presented a novel rapid,high throughput method to screen yeast with high activity and high ethanol yield. As results,6 strains with high TTF absorption value were obtained. This screening method was also very useful to screen all kinds of microorganism and plant cells.
  • XU Sha, LIU Li-ming
    Food and Fermentation Industries. 2014, 40(07): 6-10. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.013
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    During the pyruvate production by Torulopsis glabrata,the osmotic stress in culture broth gradually increased. As a result,cell growth and acid production slowed. The proteome of the T. glabrata under hyperosmotic stress was investigated by two-dimensional polyacrylamide gel electrophoresis( 2D-PAGE) and isobaric tag for relative and absolute quantitation( iTRAQ). Compared to normal condition( 860 mOsmol /kg),125,91,109 proteins were upregulated and 94,89,78 proteins were downregulated under osmolarity of 1765 mOsmol /kg,2603 mOsmol /kg,and 3324 mOsmol /kg. The protein expression involved in central metabolic pathways and energy metabolism showed apparently upregulation. Furthermore,the expression levels of superoxide dismutase and verprolin increased under hyperosmotic conditions,of which the former might due to the accumulation of reactive oxygen species( ROS),and the latter might be related to the accumulation of proline. The results presented here will facilitate the improvement of T.glabrata under hyperosmotic stress.
  • REN Xiao-pu, LI Ming-yang, REN Shao-dong, WANG Qun-xia
    Food and Fermentation Industries. 2014, 40(07): 11-15. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.021
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    As one kind of important lactic acid bacteria,Lactobacillus plantarum has attracted more and more attentions in the fields of food fermentation,industrial malolactic fermentation and healthcare. Semi-quantitative assay was used to explore the environmental factors which induced the biofilm formation by L. plantarum. Several different physical and chemical factors,and food additives were investigated and the results showed that strong positive biofilm formation by L. plantarum was obviously induced by 6% sodium chloride. Weak positive biofilm formation was induced by the weak acidic( pH 4. 8 ~ 5. 8) conditions,citrate and phosphate at the concentrations of 0. 2% and0. 4%,respectively,and the addition of gelatin. In conclusion,the biofilm formation by L. plantarum was greatly affected by some environmental factors.
  • HOU Qiang-chuan, GUO Zhuang, ZHANG Jia-chao, SUN Tian-song
    Food and Fermentation Industries. 2014, 40(07): 16-22. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.022
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    In this study,the lactic acid bacteria of two fermented vegetable samples collected from the Russian Republic of Kalmykia Elista were isolated and identified by a combinatory approach of laboratory cultivation and 16S rRNA sequence analysis. Besides,phylogenetic dendrograms were constructed based on these isolates. A total of 19strains of lactic acid bacteria were identified from these two samples. These bacteria belonged to the genera Lactobacillus( 14 strains),Pediococcus( 3 strains) and Leuconostoc( 2 strains),including Lactobacillus plantarum( 7strains),Lactobacillus brevis( 1 strain),Lactobacillus delbrueckii( 2 strains),Lactobacillus helveticus( 1 strain),Lactobacillus kefiranofaciens( 1 strain),Lactobacillus kefiri( 2 strains),Leuconostoc lactis( 1 strain),Leuconostoc mesenteroides( 1 strain),Pediococcus ethanolidurans( 2 strains),and Pediococcus pentosaceus( 1 strain). In addition,the bacterial abundance and diversity at the metagenomics level were analyzed using barcoded pyrosequencing targeting at the V1-V3 variable regions of 16S rRNA. A total of 10869 and 12204 unique sequences were analyzed from these two samples. After comparing with the Ribosomal Database,Lactobacillus and Lactococcus,which belonged to the phylum Firmicutes,were the dominant genera in the samples and accounted for 91. 66% and 3. 81% of the total number of bacteria,respectively. In addition,other genera such as Vibrio,Pediococcus,Enterococcus and Kluyveromyces in the samples were also identified herein.
  • FAN Jin-bo, XIA Lin-jing, ZHOU Su-zhen, LV Chang-xin, FENG Xu-qiao
    Food and Fermentation Industries. 2014, 40(07): 23-28. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.023
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    Pressing is the last procedure and the least understood step of semi-hard and hard cheese making. The aim of this paper was to assess the effect of the pressing time and pressure on cheese properties. The chemical composition,the texture and the rheological properties of cheeses made with different pressing parameters were investigated.And their microstructure was also studied by confocal laser microscopy. The results showed that as time and pressure were increased,moisture content of cheese samples decreased,while fat and protein content increased significantly.As the time and pressure increased,texture testing showed that the hardness and chewiness of cheese were significantly increased,while the flexibility was significantly decreased. Rheometer testing showed that the elasticity modulus of cheese increased,while the melting of cheese weakened. Cheese microstructure pictures showed that,with the extension of the pressing time,the protein network was more compact and orderly. With the increase of pressing pressure,a large number of fat globules aggregated. The mechanism of the effect of pressing on the cheese properties was also discussed.
  • CHEN Xi, WANG Jin-jing, YANG Han, LI Qi
    Food and Fermentation Industries. 2014, 40(07): 29-33. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.025
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    A total 26 kinds of commercial beer were chosen as models to investigate the effect of beer components on beer foam retention. The foam stability,total protein content,total saccharide content,total polyphenol content,viscosity,pH and alcohol were determined by the national standard,high performance liquid chromatography( HPLC) method,Bradford method and ASBC method. The results showed that total saccharide,total polyphenol,total protein content and viscosity had higher correlations with beer foam stability while pH and alcohol had lower correlations.
  • ZHANG Jing-jing, ZHENG Hui-na, ZHANG Chao-hua, HAO Ji-ming, ZHANG Ji, FAN Chao-yong
    Food and Fermentation Industries. 2014, 40(07): 34-39. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.026
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    The water-soluble protein,salt-soluble protein and insoluble protein were separated from Crassostrea rivularis Gould,and the three protein fractions were hydrolyzed by trypsin and alkaline protease. The degree of hydrolysis( DH) under different time was determined. The solubility and emulsifying capacity of protein hydrolysates with different degree of hydrolysis were investigated. The results showed that the solubility of protein hydrolysates prepared by alkaline protease was better than that by trypsin. The degree of hydrolysis was higher and the solubility was better as the hydrolysis time was longer. But protein emulsification and emulsion stability showed a rise at first followed by a decline. When the DH reached 7%,the emulsification and emulsion stability of water-soluble protein reached maximum,and the emulsifying capacity of salt soluble protein and the insoluble protein were better at the degree of hydrolysis of 5. 8% and 5. 7%,respectively. Limited hydrolysis could improve the solubility of oyster protein and improved the emulsification to a certain extent. These results will provide theoretical basis for the further use of oyster protein resource.
  • SU Wu, ZHENG Xiao-fen, XU Rui-xuan, TU Ting, JIANG Li-wen
    Food and Fermentation Industries. 2014, 40(07): 40-45. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.027
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    Dansyl chloride was used as derivative reagent and 1,7 amino heptyl amine was used as internal standard to verify the " GB /T5009. 208-2008". The soybean was chosen as raw material to find the change rule of amino nitrogen and biogenic amine of Douchi in the process of natural fermentation. The results showed that there were eight kinds of biogenic amines in natural fermentation process as follows: histamine,tryptamine,beta phenethylamine,putrescine,cadaverine,tyramine,spermine and Spermidine. After 72 h of fermentation,the content of tyramine rose to the highest( 488 mg /kg),the content of tyramine and amino acid nitrogen were greatly increased first and then slowed down gradually. Refer to the relevant provisions,it was found that the contents of histamine and tyramine which had the main toxic effect were not higher than the country maximum allowable range,so in theory there was no potential safety hazard in bacteria-fermented Douchi. And it should be noticed that the rising trend of histamine content may cause potential safety hazard,so the natural fermentation time of bacteria-fermented Douchi should be controlled.
  • HAO Rui-fang, JING Hao
    Food and Fermentation Industries. 2014, 40(07): 46-50. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.028
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    We established an asparagine /glucose( asn /glc) model reaction system and studied inhibitory effect of high and low molecular weight-lysine /glucose Maillard reaction products( H-LGP and L-LGP) on acrylamide formation and its antioxidant activity in the model reaction system. High performance liquid chromatography and radical scavenging rate method were applied. The results showed that H-LGP and L-LGP inhibited acrylamide contents effectively in the asn /glc model system at 150 ℃,with the maximum inhibition rate of 32. 0 % and 31. 4 % at 10 mg /mL,respectively. Moreover,H-LGP and L-LGP at 10 mg /mL could also increase DPPH and ABTS radical scavenging rate in asn /glc model system. In conclusion: lysine /glucose Maillard reaction products could effectively inhibit acrylamide formation and increase antioxidant activity in asn /glc model system.
  • ZHANG Xiao-li, JIANG Yu-jian, LI Feng
    Food and Fermentation Industries. 2014, 40(07): 51-55. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.029
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    The bulging of soy sauce bags always happened in spring and summer,which is mostly caused by microorganisms. In this paper,high glucose medium,nutrient agar,MRS medium,and potato sucrose medium were used to isolate microorganisms from the explosion soy sauce samples produced from Shaoxing. Only 7 kinds of bacterial strains named as k1,k2,k3,k4,k5,k6,k7were purified from nutrient agar. The results form gas production experiments confirmed that only k2strain can produce gas in soy sauce. Based on the systematic analysis of its colony morphology and characteristics,physiological and biochemical characteristics according to Bergey's Manual of Determinate Bactertology,strains k2was identified as Bacillus megaterium.
  • LIAO Yun-li, XIA Jin-mei, LIU Rong-li, XU Jian-zhong, XU Xun, XU Chen
    Food and Fermentation Industries. 2014, 40(07): 56-61. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.014
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    The optimum flocculation were screened from the different kinds of flocculations through comparison of610nm light-transmittance,the filtration rate,the removal ratio of protein and polysaccharose. Using Al2( SO4)3and poly-AlCl3 as flocculation,their effects on the extraction of the secondary metabolites from marine bacterias were investigated by HPLC( high-performance-liquid-chromatograhy). The effects of four kinds of solid-liquid separation ways on the fermentation borth of marine bacteria were compared. Results showed that the coupling method of flocculation and membrane ultrafiltration was adaptable to highly active and rapid pretreatment of different types of marine bacteria.
  • YANG Hui, DANG Cui-hong
    Food and Fermentation Industries. 2014, 40(07): 62-66. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.030
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    In order to solve the problem of immediately stopping fermentation for a low-alcohol Malus micromalus Makino wine brewed by sugar-remaining fermentation technology,the concentrated Malus micromalus Makino juice was used as a raw material. After the thick juice was diluted into thin juice,the fermentation was conducted at the temperature of 20 ℃. When alcohol content reached 5% vol,the fermentation was stopped immediately through following methods: ① SO2aqueous solution was added directly into the brewing liquor and the concentration of SO2in liquor was 360mg /L or more. ②Fermenting wine was pasteurized at 68 ℃ for 10 min. ③ The yeast in fermenting liquor was separated at a speed of 4 000 r /min by cool centrifugation at- 1 ℃ for 10 min and then cooled down to 4 ℃or SO2aqueous solution was added into the obtained wine to the final content of 60 mg /L or more. Results showed that all of the fermentation-stop methods mentioned above could stop the fermentation of wine efficiently. Took the quality of the wine into consideration,centrifugal separation of yeast and then cooling of the obtained wine or addition of 60mg /L SO2was a more appropriate method.
  • HUA Xue-yan, LI Shuo, TANG Ke-hua, LIU Wen-zhu
    Food and Fermentation Industries. 2014, 40(07): 67-71. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.031
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    The leaves residual and bark of Eucommia ulmoides Oliver was used to culture Hericium,Flammulina velutipes and Pleurotus ostreatus,some rules of the degradation of hemicellulose,cellulose and lignin had been got. The mass fraction of hemicellulose,cellulose and lignin was 9. 834 mg /g,38. 997 mg /g and 164. 667 mg /g in the leaves residual,32. 297 mg /g,108. 313 mg /g and 44. 667 mg /g in the bark of Eucommia ulmoides Oliver. When Hericium was cultured 68 days,the mass fraction of hemicellulose,cellulose and lignin was dropped 61. 0%,80. 3% and 12. 5%.When Flammulina velutipes was cultured 61 days,the mass fraction was dropped 37. 3%,82. 5% and 23. 1%. When Pleurotus ostreatus was cultured 58 days,the mass fraction was dropped 30. 3%,40. 7% and 57. 1%. The results of solid state fermentation of the Eucommia ulmoides bark showed that the full culturing time of liquid spawn was 4 days in advance than that of solid spawn when Hericium was cultured,while there was only 2 days when the Flammulina velutipes and Pleurotus ostreatus were cultured. On the degradation of lignin,the solid spawn of Hericium and Pleurotus ostreatus were better,while the two types of spawns of Flammulina velutipes had little difference and the effects were poor.On the degradation of cellulose,the liquid spawn of Hericium and Flammulina velutipes were better,but the two types of spawns of Pleurotus ostreatus had little difference and the effects were poor. When the degradation of hemicelluloses was mentioned,two types of spawns of the three edible fungi had almost the same capabilities.
  • KANG Lian-hu, LI Lü-mu, SI Xiong-yuan, LI Bin, GUO Wen-jie, MU Hua, DING Xiao-ling, XU Fa-zhi
    Food and Fermentation Industries. 2014, 40(07): 72-76. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.015
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    Screening of the strains for solid-state aerobic fermentation of the residue from wheat alcohol progressing and the antioxidant activity of small peptide from the fermentation were studied. Using Bacillus licheniformis D-1and Bacillus subtilis KG109 as strains to ferment and compare with koji used by the company at present,the amount of inoculum and levels of moisture content were tested. The result with higher moisture and more small peptides was optimum combination. Then DPPH scavenging activity,hydroxyl radical scavenging activity and the total reducing capacity of its small peptides were measured. The results showed that D-1 was the best when inoculums was 3% and moisture content was 70%,and the content of acid soluble proteins and crude proteins in the product were both significantly higher than those in the control( P < 0. 01). Five peptides with 8,7,5,4 and 3 amino acids respectively were separated from the acid soluble proteins. The other four peptides all had DPPH radical scavenging activity and hydroxyl radical scavenging activity except peptide with 8 amino acid residues,and the total reducing capacity of 5 mg /mL of peptide with 4 acid residues was equivalent to 0. 5 mg /mL glutathione. These results implied that D-1 was an efficient strain to improve the forage quality of the residue from wheat alcohol progressing and most of small peptides had strong antioxidant activity.
  • FAN Jin-bo, CAI Xi-tong, FENG Xu-qiao, HOU Yu, YU Xiao-feng, JIANG Mo
    Food and Fermentation Industries. 2014, 40(07): 77-83. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.002
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    The antioxidant activity of caffeic acid,rutin,resveratrol,phloretin,and phlorizin were evaluated compared with VC,BHT and EDTA. Five areas were tested: the total antioxidant capacity,DPPH free radicals scavenging activity,ABTS free radicals scavenging activity,ferric reducing ability and ferrous ions chelating activity. The results indicated that caffeic acid showed strong total antioxidant capacity,DPPH radical scavenging activity( IC500. 023 ± 0. 004 mg /mL) and ferric reducing ability,the total antioxidant capacity reached 1. 873 ± 0. 022 mg VCeq /mg at a concentration of 0. 1 mg /mL; Resveratrol showed a strong ABTS radical scavenging activity( IC500. 056 ±0. 006 mg /mL),significantly higher than that of BHT and VC( P < 0.01). Rutin showed the strongest ferrous ions chelating activity,followed by resveratrol. Other phenolic compounds showed little ferrous ions chelating activity. All the above tests showed 5 compounds have the potential natural antioxidants.
  • CHEN Hong-mei, XIE Ling
    Food and Fermentation Industries. 2014, 40(07): 84-87. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.003
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    The enzyme activity of polyphenol oxidase( PPO) from Poria cocos was detected by spectrophotometry. The effects of pH value,temperature,substrate concentration and inhibitors on PPO activity were studied. The results indicated that the optimum pH value,temperature,and substrate concentration for PPO were 6. 5,35 ℃,and0. 06 mol /L,respectively. The kinetics of enzyme-catalyzed reaction of PPO complied with the law of Michaclis equation. The Kmand Vmaxwere 0. 024 4 mol /L and 121. 95 U /min,respectively. The activity of PPO was inhibited by sodium chloride,ascorbic acid,L-cysteine and citric acid. The inhibitory effects against PPO activity were increasing with the increasing concentrations. The order of inhibitors showing effects from strong to weak was as follows: L-L-cysteine,ascorbic acid,citric acid and sodium chloride.
  • LIU Ying, ZHAO Jie, XU Lin
    Food and Fermentation Industries. 2014, 40(07): 88-91. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.032
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    In this paper,taking Flammulina velutipes as the material,the polysaccharide is extracted by microwave-assisted method,and acetylated polysaccharide derivatives are prepared,and then its oxidation resistance test is carried out. A variety of system of free radical scavenging experiment results show that the acetylated Flammulina velutipes polysaccharide with different concentrations have scavenging effects on ·O2-,·OH,DPPH·. Compared with the free radical scavenging capability of the sample of Vc,the clearance rate of polysaccharides on ·O2-is almost the same as that of Vc. The reducing power and the ability to clear ·OH and DPPH· of the acetylated Flammulina velutipes polysaccharides are slightly weaker than Vc. Experimental studies have shown that acetylated flammulina velutipes polysaccharides have strong antioxidant activity,and it can be considering to be used in food and drugs production together with other natural antioxidants.
  • GUO Li-qiong, WU Hou-jiu, DOU Hua-ting
    Food and Fermentation Industries. 2014, 40(07): 92-96. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.004
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    The 5-methyltetrahydrofolate( 5-MTHF) content in different fruit tissues of nine citrus varieties were determined by Reversed-phase High Performance Liquid Fluorescence Detection method( RP-HPLC-FLD). Results indicated that 5-MTHF content in the pulp of sweet oranges( Citrus Sinensis) was the highest,especially that in the Hamlin pulp was up to 29. 28 μg /100g; followed by pummelo varieties( C. grandis),the content of 5-MTHF in Shatian pummelo pulp was 23. 45μg /100g,liangping pummelo 15. 01μg /100g; Mandarin fruits had lower content of5-MTHF,Satsuma Mandarin pulp had 15. 58μg /100g,Taiwan Ponkan only 8. 92μg /100g; the lowest content of 5-MTHF was in Eureka Lemon pulp,only 4. 1μg /100g. In general,the content of 5-MTHF in different fruit tissues from high to low was: seed > pulp > segment wall > peel.
  • LI Qiang, LI Xiao-lin, HUANG Wen-li, XIONG Chuan, YANG Zhi-rong, ZHENG Lin-yong
    Food and Fermentation Industries. 2014, 40(07): 97-100. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.033
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    In order to understand the differences in nutrition of Tricholoma matsutake from seven different places in Sichuan Province,China,we measured the content of protein,fat,fiber,mineral elements,vitamin B,and amino acids of T. matsutake fruit bodies according to the national food sanitation standard. The clustering analysis of these T.matsutake were completed by the software SPSS. Furthermore,the nutrition of T. matsutake in Sichuan was compared with the others from Congqing,Yunnan,Tibet and Heilongjiang. The results indicated that the average protein of T.matsutake from Sichuan was 21. 09%. In addition,all of the T. matsutake had low fat content with average 3. 28%.The average content of fiber was 18. 28%. The content of calcium and iron was relatively high( average content with249. 21 mg /kg and 880 mg /kg,respectively). The mineral elements content of T. matsutake varied greatly in different region. For example,the calcium content of fruit bodies from Mianning was more than 700% of which from Huidong. While,the iron content of fruit body from Yanbian was more than 600% of which from Huidong. The T. matsutake in all regions could be divided into two groups. The T. matsutake from Huidong,Mianning,Muli and Yanyuan were clustered in one group indicting they had high content of protein and fat. While the T. matsutake from Xiaojin,Yanbian and Yajiang were clustered into another group indicting that they had high content of fiber and mineral elements. The results showed the quality of T. matsutake in Sichuan was better than which in other places. In addition,the nutrients and mineral elements content of T. matsutake could be affected by environmental and soil conditions.
  • LI Song-tao, YIN Zhi-feng, GAO Yang, HAO Ting, WANG Liang-you
    Food and Fermentation Industries. 2014, 40(07): 101-105. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.005
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    Konjac glucomannan and gallic acid were used as the raw materials to prepare gallic acid konjac glucomannan. The effects of the amounts of N,N'-Diisopropylcarbodiimide and 4-dimethylaminopyridine,mass rate of triacetyl gallic acid and konjac glucomannan,reaction temperature and reaction time on the esterification reaction were investigated through single factor and orthogonal experiments. The results showed that optimal reaction conditions were determined as follows: mole rate of triacetyl gallic acid,N,N'-Diisopropylcarbodiimide and 4-dimethylaminopyridine was 1∶ 1. 5∶ 0. 1; mass rate of triacetyl gallic acid and konjac glucomannan was 2∶ 1; reaction temperature and reaction time were 100 ℃ and 4 h respectively; and the average degree of esterification of triacetyl gallic acid konjac glucomannan was 0. 593. In contrast with konjac glucomannan,both viscosity and stability of the hydrosol of modified konjac glucomannan product were remarkably improved.
  • FAN Fang-yu, CHANG Yan-qiong, DONG Xuan-gang, KAN Huan
    Food and Fermentation Industries. 2014, 40(07): 106-110. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.016
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    Characteristic of microencapsulated camellia seed oil was studied. The oil was produced by spray drying,and used maltodextrin and soybean protein isolate( SPI) as the wall material. Fine surface of microcapsules were observed under scanning electron microscopy( SEM),and shriveled microcapsules were observed in 30 day under 40℃ and 60℃. This indicated that microcapsules cannot store up in long time and high temperature. The value of Tmof microencapsulated camellia seed oil was 157. 2 ℃ determined by differential scanning calorimetry( DSC). This showed good stability and broad application range. The value of decomposion of microencapsulated camellia seed oil was 280 ℃ determined by TG,which indicated the processing temperature can not exceed 280℃. The retention ration of microencapsulated camellia seed oil was measured at different temperature and relative humidity using Avrami's equation and result showed microencapsules should store up in lower temperature and lower relative humidity.
  • WANG Yuan, JIA Ge, WANG Meng, CHENG Ni, CAO Wei, ZHAO Jing
    Food and Fermentation Industries. 2014, 40(07): 111-114. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.034
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    Honey is not only considered a part of traditional Chinese medicine,but is also valued as a functional food. Studies have shown that honey has both antibacterial and anti-inflammatory properties,useful in treatment of burn and wound healing as well as childhood cough etc.. These functions may be related to its antioxidant activity. In this paper,we reviewed the antioxidant activity of honey both in vitro and in vivo.
  • GUO Xi-juan, YANG Ming-duo, SHI Wen-hui, GUO Yan
    Food and Fermentation Industries. 2014, 40(07): 115-120. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.046
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    Extract: foreign countries traditional food culture and food industrialized features,as well as industrialization development status were discussed. Based on the analysis of the foreign countries traditional food development,the ideas of bringing the local traditional food characteristics into the industry as well as the consumer psychology research and food culture inheriting and carrying forward were proposed.
  • LI Qiang, LIU Wen, DAI Yue, LIU Peng, DUAN Min
    Food and Fermentation Industries. 2014, 40(07): 121-125. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.047
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    Risk classification and supervision in food enterprise is common in foreign countries. This paper analyzed the risk classification system of food enterprise in Germany,discussed the legislation,implementation,operation system and the risk classification methods. This paper gives advices on the establishment and implementation of the same system in China.
  • XU Li-jing, GAO Li-pu, WANG Qing, ZUO Jin-hua
    Food and Fermentation Industries. 2014, 40(07): 126-130. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.006
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    Natural polysaccharides are abundant,low price and simple preparation. It has a promising application. This article summarized the types of natural polysaccharides coatings and its preservation mechanism. The applications in edible fungi preservation are reviewed. Meanwhile,the problems and trends in research were proposed.
  • BAI Xiao-ming, WANG Hua, ZENG Xiao-feng, LONG Yong
    Food and Fermentation Industries. 2014, 40(07): 131-135. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.035
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    Recent development of juice concentration technology,including the application of vacuum evaporation,freeze concentration,membrane technology in concentrated fruit juice process was summarized as well as the development situation. Osmotic distillation,membrane distillation and integrated membrane technology are illustrated.And their advantage and disadvantages were compared. From the perspective of the juice market expansion and product quality,membrane technology application future was also proposed.
  • ZAHO Xiu-ling, WU Di-di, ZHU Qi-hang
    Food and Fermentation Industries. 2014, 40(07): 136-143. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.036
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    The paper summarized the latest research advances of its function components from Folium Nelum binis and its extraction,separation and purification. The paper focused on the application in the beverage industry and functional componets extraction,separation and purification. It is hope that the research can provide the basic information for the further research of food and medical functions of Folium Nelum binis.
  • WANG Li-na, HU Xue-lian
    Food and Fermentation Industries. 2014, 40(07): 144-148. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.007
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    A method for the determination of phenolic acids in beer based on solid-phase extraction( SPE) and high performance liquid chromatography( HPLC) was developed. The beer samples were purified with Oasis HLB solid phase extraction cartridges,and the separation of 15 phenolic acids compounds including gallic acid were performed on a ZORBAX ODS column( 4. 6 mm ×250 mm,5 μm) with the mobile phase of methanol( 0. 1% acetic acid) /water( 0. 1% acetic acid) in a gradient elution mode. The flow rate of the mobile phase was 1. 0 mL/min,and the detection wavelength was280nm. The recoveries were ranged from 90% ~118%,and the relative standard deviations( RSD) were less than 5. 0%.The method was simple,accurate,and sensitive and suitable for the determination of phenolic acids in beer.
  • WANG Lu, JIANG Wei, LIU Xiang, WANG De-liang, LIU Yu-mei
    Food and Fermentation Industries. 2014, 40(07): 149-155. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.008
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    In this study,a method developed by our lab based on gas chromatography – mass spectrometry( GC/MS) coupled with headspace solid-phase micro-extraction was used to trace the content changes of( R)-(-)-linaloo,( S)-( +)-linaloo,S-(-)-β- citronellol,R-( +)-β-citronellol,( +)-α-terpineol,(-)-α-terpineol,geraniol and nerolidol derived from hops. The influences of different hopping regimes were evaluated during the mash process. As a result,compared to the before the end of boiling 10 min- hopping,the late-hopping,whirlpool tankhopping and fermetation end-hopping could significantly enhanced the remaining content of terpenols in the beer. The contents of( R)-(-)-linaloo,geraniol,R-( +)-β-citronellol,and nerolidol were 1. 94 ~ 14. 39 μg /L,9. 83 ~12. 10 μg /L,2. 91 ~ 6. 79 μg /L,and 5. 34 ~ 6. 81 μg /L,respectively. Their OAV were found to be in the range of0. 29 ~ 6. 54. So,we further investigated the biotransformation of four alcohols during fermentation of hopping regimes. Combined with the sensory evaluation of 4 hopping regimes for flavor in beer,it showed that whirlpool tankhopping produced better hoppy aroma than others.
  • LIANG Hui, XUE Zheng-lian, ZHOU Yang, WANG Dong-dong, YE Yang
    Food and Fermentation Industries. 2014, 40(07): 156-160. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.009
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    In view of the mutants of lactic acid bacteria obtained by mutagenesis were massive and the traditional determination of GABA content in 96-well plates through spectrophotometer was low efficiency and high cost,microplate reader was used to determine the content of GABA quickly and efficiently herein and the main influencing factors of the method was optimized based on the Berthelot reaction. The optimum condition for the GABA determination was as follows: 0. 75mL 0. 2mol /L pH10. 0 borate buffer,2. 5 mL 6% redistilled phenol,and 2mL NaClO solution with5. 2% available chlorine. Under this condition,the higher concentration of GABA could be detected by microplate reader. The precision test was implemented simultaneously. Finally,in order to verify the feasibility of this method,75 mutant strains were fermented in 96-well plates and then respectively measured by spectrophotometer and microplate reader. The data regression showed a significant relationship between these two methods,and the correlation was0. 9493. The experimental results showed that microplate reader can simply,rapidly and accurately measure the content of GABA. It also laid the foundation for high-throughput selecting and breeding of high-yield GABA from lactic acid bacteria in future.
  • MENG Ling-shuan, XU Xin, LIU Qian-ying, SONG Xue-fei, ZHOU Ting-ting, WU Ri-na
    Food and Fermentation Industries. 2014, 40(07): 161-165. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.037
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    pH,acidity,mass concentration of free amino nitrogen and viable count of lactic acid bacteria of yogurt were tested under refrigerated conditions. The changes during the shelf life were compared. The results showed that during the yogurt shelf life,pH decreased,acidity increased,viable count of lactic acid bacteria was declined,nitrogen concentration of free amino acid increased a little in the beginning of refrigerated storage and then decreased with the frozen time. Sensory evaluation of decreased,but all indicators met national standards.
  • ZHANG Huai-yu, WANG Jun-jie, CHEN Yuan-fan, WEI Jin-mei, LI Zhen-zi, Fang Shou
    Food and Fermentation Industries. 2014, 40(07): 166-172. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.017
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    Extraction of steam distillation for WU-du Zanthoxylum bungeanum( cv. Dahongpao) essential oil was optimized by single factors analysis and central composite design( CCD). The results indicated that the optimistic parameters of essential oil extraction were : the material-water ratio 0. 11( m /v),the vapor volume 73 mL and the distillation time 96 min. Under these conditions the extraction yield of essential oil was 6. 71%. Essential oil from the optimum technology was analyzed by gas chromatography-mass spectrometry( GC-MS). Seventy-three volatile components were separated,and 31 of them were identified,accounting for 95. 16% of the total volatile components. The main ingredients for the fragrance were limonene 20. 204%,linalool 16. 645% and linalyl anthranilate 19. 733%,and the relative content of linalyl anthranilate was higher in WU-du Zanthoxylum bungeanum( cv. Dahongpao).
  • LI Hui, YAO Su, LIU Yang, BAI Fei-rong, ZAHI Nagi, CHENG Chi
    Food and Fermentation Industries. 2014, 40(07): 173-176. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.020
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    The isotopic composition of the elements N and C of twelve Ophicordyceps sinensis samples and three Cordyceps hawkesii samples was determined by isotope ratio mass spectrometry. The statistical analysis results showed that the δ13C value from different regions were overlap,but the δ15N value ranges were different and distinguished in almost all of the samples( except sample from Hainan of Qinghai). The δ15N value from yushu and guoluo were significantly different from other regions( P < 0. 009). the δ15N and δ13C values between Ophicordyceps sinensis and Cordyceps hawkesii were significantly different. Therefore,Nitrogen stable isotope ratio analysis is useful in rapidly differentiating the geographical origin of Ophicordyceps sinensis. The method will have a wide application in tracing the origin of Ophicordyceps sinensis.
  • ZHANG Ru-jiao, HE La-ping, LI Cui-qin, ZHANG Ling, WANG Meng, ZHU Qiu-jin
    Food and Fermentation Industries. 2014, 40(07): 177-181. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.001
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    In order to measure the degradation rate of cholesterol of five strains in vitro,o-phthalalehpyde( OPA)method and High performance liquid chromatography( HPLC) method were used to determine the cholesterol of the medium respectively. Linearity,precision and recovery between the two methods were compared. The results were as follows:the degradation rate of cholesterol of five strains are among 12. 34% ~ 28. 57%,two methods both had good linearity in the rang of 0 ~ 0. 20 mg /mL and 0 ~ 0. 70 mg /mL respectively,precision of relative standard deviation was 0. 32%( n =5) and 0. 96%( n = 5),the spike recoveries were both between 99. 33% and 100. 18%. Overall comparison indicated that both the OPA method and HPLC method can measure the degradation rate of cholesterol of bifidobacteria.
  • WANG Wei, ZHANG Yong-jing, JIANG Zhong-hui, GU Zheng-fen, LIU Wei, YE Chuan, LIN Lin, JIANG Shao-tong, LU Jian-feng
    Food and Fermentation Industries. 2014, 40(07): 182-188. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.038
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    Shenxian( fairy) bean is a traditional fermented soybean product especially popular in the north Anhui region. To study the nutrition and volatile components of Shenxian bean,the national standard method,head-space solid phase micro-extraction( HS-SPME) and GC-MS analysis were applied. The results indicated that the contents of basic nutrition components of Shenxian bean are very close to natto. A total of 72 compounds were identified and they were classified into nine major groups. Major classes of compounds included alcohols( 16),nitrogenous( 8),esters( 2),aldehydes( 14),hydrocarbons( 18),ketones( 4),acids( 3),phenols( 4) and ethers( 3). Furthermore,total free amino acids( FAA) of Shenxian bean reached 4. 70 mg g-1 with the highest glutamic acid concentration. In conclusion,Shenxian soybean product has a good flavor and higher nutritive values.
  • FAN Zhen-jiang, TAO Yan-juan, ZHOU Kun
    Food and Fermentation Industries. 2014, 40(07): 189-192. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.010
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    The purpose of this article was to detect the selenium content of eggs and to explore the possibility of eggs as selenium source or selenium enriched foods. A method of hydride Generation-Atomic Fluorescence Spectrometry( HG—AFS) for the determination of selenium in eggs was proposed. Amount of selenium in eggs was determined by HG—AFS after the samples were microwaved digestion. The detection limits of this method was 1. 6 μg /100g,and the recoveries were in the range from 91. 3% to 104. 1% with precision( n = 7) of 1. 6% to 3. 8%. The amount of selenium in eggs were in the range from 13. 3 μg /100g to 23. 6 μg /100g,and the amount of selenium in yolks were in the range from 26. 6 μg /100g to 32. 5 μg /100g. The amount of selenium in yolks was fourfold amount of selenium in albumen. The sequence of amount of selenium in four eggs was goose egg,quail egg,duck egg and hen egg. Ducks egg and hen egg with selenium Nutrition Reference Values%( NRV%) greater than 15%,and is a selenium source or selenium contained food. Gooses egg and quails egg were rich in selenium with selenium NRV% greater than 30%.Yolks of four eggs were all rich in selenium with NRV% greater than 30%.
  • WU Man-gang, WU Xue-yan, YU Hai, GE Qing-feng, WANG Zhi-jun
    Food and Fermentation Industries. 2014, 40(07): 193-198. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.039
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    Sarcoplasmic proteins and myofibrillar proteins were extracted from Chinese sausage. Protein carbonyl,sulfydryl group contents and protein turbidity,protein degradation and solubility were measured and the effect of protein oxidation on aggregation,proteolysis and solubility during drying process was studied. The protein carbonyl content was increased and SH group number was decreased. Protein turbidity and Surface hydrophobicity( H0) increased,which was associated with the degree of protein oxidation. Proteolytic susceptibility was influenced by the degree of protein oxidation. The protein solubility determinations suggested that hydrogen bonds,hydrophobic interactions,disulphide bonds and ionic bonds are the main bonds in forming three-dimensional network structure of sausage proteins.
  • DONG Qing-li, YAO Yuan, ZHANG Yan, Aclaoha E C Ihekwaba
    Food and Fermentation Industries. 2014, 40(07): 199-204. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.040
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    In order to verify the effect of sodium lactate( NaL) amount on the pork's proteins expression and stability,the pork's longissimusdorsi muscle was soaked for 5 h in different concentrations of NaL solution( 1%,2%,3%,4%,5%,10%,20% and 30%,respectively). Sodium dodecyl sulfate polyacrylamide gel electropheresis( SDS-PAGE) and differential scanning calorimetry( DSC) analysis were then applied. Results showed that,compared with the control treatment,NaL had little effects on myogen of longissimusdorsi muscle. However,NaLsolutions with high concentration( 10%,20% and 30%) affected the expression of actin( 43 kDa) and myosin heavy chain( 190 kDa),respectively. Meanwhile,the denaturation temperature of actin and myosin was decreased in high-concentration of NaL solutions. In a conclusion,protein expression in pork's longissimusdorsi muscle might be affected by NaL amount,but low-concentration of NaL used as meat preservative does not have big effect on meat quality.
  • ZHANG Hu-bin, HE Jian-ye, WANG Zhi-geng, MEI Lin, XUE Xiu-heng
    Food and Fermentation Industries. 2014, 40(07): 205-211. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.011
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    In our study,electronic nose was used to measure the flavor finger-printing changing of Chinese fermented sausage in the process of fermentation( Day 0,2,6,11,15). The relevance between chemometry quality indicator( Total volatile Basic Nitrogen( TVB-N),Peroxide Value( POV)) and flavor finger-printing was studied by the way of principal component analysis( PCA),linear discriminant analysis( LDA) and model analysis which based on partial least squares method( PLS). The sensor array of PEN3 electronic nose showed the most outstanding capability distinguishing the flavor changing during the sausage fermentation under the conditions as follows: temperature35℃,headspace volume 100ml,accumulation time 30min,data-collection interval 55-60s. In addition,the relationship between flavor finger-printing changing and chemometry modification was also identified. Our study provided a novel PLS prediction model for sensitive and precise detection of chemometry indexes of Chinese fermented sausage by flavor finger-printing identification. In a mock test for 25 samples,the standard errors of TVB-N and POV measured by this new method were 0. 1557 and 0. 0030 respectively.
  • SUN Xiao-mei, DAI Jun, CHEN Shang-wei, ZHU Song
    Food and Fermentation Industries. 2014, 40(07): 212-217. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.041
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    We investigated the optimal extraction condition of Ganoderma lucidum( G. lucidum) fruiting bodies polysaccharides by comparing the four extraction methods of ultrasonic-assisted extraction,microwave-assisted extraction,accelerated solvent extraction and water bath reflux extraction. The simultaneously molecular weight distribution of polysaccharides extracted under different extraction conditions were analyzed by high performance size exclusion chromatography. Results showed that the yield of polysaccharides extracted under ultrasonic assisted condition was higher,and the structure of polysaccharides was less damaged. Moreover,molecular weight distributions of polysaccharides from different G. lucidum fruiting bodies by ultrasonic-assisted extraction were determined and compared.The results showed that the differences were obvious,and the strains,cultivation and geographical regions all had influences on molecular weight distribution.
  • LIU Lu-ping, XIE Cui-pin, YUE Li, JING Si-qun, CAI Yuan-qiang, TONG Lian-hua
    Food and Fermentation Industries. 2014, 40(07): 218-222. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.018
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    In order to improve the extraction rate and quality of Portulaca oleracea seeds oil,the extraction rate and physicochemical properties of Portulaca oleracea seeds oil were selected as the indexes,ultrasound-assisted enzyme hydrolysis method was used in the pretreatment of Portulaca oleracea seeds and Soxhlet extraction method was applied on the oil extraction. The optimum conditions of enzyme hydrolysis pretreatment were determined using orthogonal trial based on single-factor experiments. gas chromatography /mass spectrometry( GC-MS) was used in the analysis of the fatty acid components of the oil extracts with the new method and compared with the tradition extraction way.The results showed that the optimum conditions of enzyme hydrolysis pretreatment were: enzyme dosage 2%,hydrolysis temperature 50 ℃,pH6. 0,liquid to solid ratio 6∶ 1. Under these conditions,the extraction rate with the combination method was up to 86. 8%,increased by 15. 43% compared to Soxhlet extraction method. The seed oils extracted by two methods both contained variety of unsaturated fatty acids. And the content of unsaturated fatty acids was over85%,content of linolenic acid was 39%.
  • CUI Hui-ling, CHEN An-jun, LUO Chan, XIA Jiao, DONG Wei, SUN Meng-yuan
    Food and Fermentation Industries. 2014, 40(07): 223-229. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.042
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    The objective of this study was to analyze the dominant spoilage bacteria of fresh-cut lettuce stored at4 ℃ for 10 days. Based on the surface bacterial species of raw lettuce combined with sensory changes and microbial count and morphology,the possible dominant spoilage bacteria were found preliminarily. In the vitro experiments,10mL broth cultured about 18 h was dissolved in 990 mL physiological salinewith solid-liquid ratio 1∶ 10,in which the processed fresh-cut lettuce were suspended 15 s,and the samples was respectively stored at 4 ℃ for further determination of the dominant spoilage bacteria typically based on physiological and biochemical experiments and molecular identification. The results indicated that Exiguobacterium( M1-1 and P1),Enterobacteriaceae( V4 and M1-4),Bacillus( M3) isolated from the raw lettuce,and Exiguobacterium( M1-1),Acinetobacter( M1-2 and P2),Aeromonas( M1-3),Pantoea( N1 and V3),Enterobacteriaceae( V2) isolated from the fresh-cut lettuce stored at 4℃ were dominant spoilage bacteria. Furthermore,Exiguobacterium( M1-1) was the specific spoilage bacteria. Therefore,it was very important to analyze species and living conditions about the initial bacteria of raw lettuce and dominant spoilage bacteria of fresh-cut lettuce,to control the quality of fresh-cut lettuce and shelf life.
  • CHEN Shuang-ying, ZHAO Xi-heng, LIU Hong-zhu, LI Jin-cai
    Food and Fermentation Industries. 2014, 40(07): 230-234. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.043
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    Preservation effect of gibberellic acid( GA) and 6-benzyladenine( 6-BA) treatments on fresh-cut broccoli was studied. Fresh-cut broccoli was dipped in 120 mg /L GA and 50 mg /L 6-BA for 3 and 5 min respectively. Antioxidative enzyme activities,content of antioxidative substance,active oxygen level during cold storage at 8 ℃were investigated. The results indicated that GA and 6-BA treatments effectively enhanced activities of superoxide dismutase,catalase and ascorbate peroxidase activities,and inhibited activity of peroxidase and delayed the decrease of ascorbic acid and carotenoid of fresh-cut broccoli florets. The GA and 6-BA treatments restrained superoxide anion and hydrogen peroxide accumulation and maintained chlorophyll and soluble protein content,and inhibited malondialdehyde accumulation of fresh-cut broccoli florets. The effect of 6-BA treatment was better than that of GA treatment.Physiological effects of inhibiting senescence of GA and 6-BA treatments in fresh-cut broccoli associated with improving the antioxidative properties and alleviating active oxygen damage to biological macromolecules.
  • MA Xiao-jun, JIA Ke-hua
    Food and Fermentation Industries. 2014, 40(07): 235-240. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.044
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    Barley beverage was prepared by thermostable amylase hydrolysis. In order to choose appropriate stabilizers,the influence of emulsifiers and hydrocolloids on the stability of barley emulsion was studied by single factor expreiment. Then the main emulsion components were studied by response surface methodology( RSM). The physicochemical properties considered as response variables were zeta potential,emulsion stability( ESI),polydispersity index( PDI). The overall optimum component is: 0. 2% Citrem,0. 1% SMS,0. 9% rice bran oil,0. 07% xanthan gum and 0. 3% carrageen.
  • LIU Meng-jia, ZHOU Qiang, LIN Ge-qun, HUANG Li-qing, LIN Li-ling, YANG Wen-kuan
    Food and Fermentation Industries. 2014, 40(07): 241-245. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.019
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    Using chestnut milk and milk as raw material,the processing of chestnut-Kefir fermented milk were studied in details in this essay. The optimal fermentation conditions were determined by L9( 34) orthogonal tests. The results showed that alcohol content was 0. 21% and acidity was 86. 98 ° T under the following conditions: chestnut milk content 15%,inoculation volume 8%,and fermented at 25 ℃ for 19 hours. The final product possessed smooth taste,mild acidity,gentle and specific flavor.
  • XU Su-yun, LUO Yu, YAO Min, GONG Pan, DING Zhu-hong
    Food and Fermentation Industries. 2014, 40(07): 246-250. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.07.045
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    This paper was studied to the producing technology of buckwheat low-fat low sugar walnut milk,taking walnut dregs and Buckwheat power as main raw material. The parameters of processing technology were determined through orthogonal tests and sensory evaluation. The results showed that the optimal formula of buckwheat low-fat low sugar walnut milk was with 28% walnut dregs juice,1. 5% Cooked buckwheat powder,3% sucrose and2. 8% erythritol. And the product with good taste and flavor was obtained,whose best stabilizer for the beverage was 0. 05% CMCNa,0. 12% xanthan gum and 0. 08% sodium alginate.