15 February 2021, Volume 47 Issue 3
    

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  • ZOU Renying, ZHU Huiyue, XU Mengshu, TIAN Peijun, ZHANG Hao, ZHAO Jianxin, CHEN Wei, WANG Gang
    Food and Fermentation Industries. 2021, 47(3): 1-9. https://doi.org/10.13995/j.cnki.11-1802/ts.025503
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    “Psychobiotics” are a class of probiotics that have regulatory effects on neurobehavioral functions. With the maturity of the “microbiota-gut-brain axis” theory, psychobiotics have been widely used in the prevention and treatment of psychiatric diseases. In this study, mice under the chronic unpredictable mild stimulus (CUMS) were treated with multiple probiotics (Bifidobacterium breve CCFM1025, Bifidobacterium infantis CCFM687, and Pediococcus lactis CCFM6432) for four weeks. Multiple probiotics intervention improved the sucrose preference, reduced the immobile time in forced swim test, and increased the activity in open arms of elevated plus maze of the chronically stressed mice. The mechanism of action correlated with the down-regulation of serum corticosterone and pro-inflammatory cytokines (TNF-α and IL-1β) in the prefrontal cortex. Chronically stressed mice also showed significant constipation symptoms. Probiotics treatment increased the defecation speed which was possibly through the elevation of cecum short-chain fatty acids, 5-hydroxytryptamine (5-HT) and Htr4 gene expression. Besides, significantly positive correlations were observed between the intestinal L-tryptophan (L-Trp) and brain L-Trp/5-HT levels in the mice treated with probiotics, which indicates that the regulation of intestinal and brain L-Trp/5-HT synthesis may contribute to both constipation and depression. This study confirms the psychobiotic potential of the multiple probiotics, expands the application of probiotics, and provides novel insight for the treatment of depression and accompanied constipation.
  • LI Chenchen, LI Mengli, JIANG Bo, ZHANG Tao
    Food and Fermentation Industries. 2021, 47(3): 10-17. https://doi.org/10.13995/j.cnki.11-1802/ts.025160
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    As a kind of prebiotics, 2'-fucosyllactose (2'-FL) can be added to infant formula, and is essential for the development of the intestinal flora and immune system of infants. The synthetic pathway of 2'-FL was established by heterologously co-expressing L-fucose kinase/GDP-L-fucose pyrophosphorylase gene fkp and α-1,2 fucosyltransferase gene futC in Escherichia coli BL21star (DE3), which were derived from Bacteroides fragilis and Helicobacter pylori respectively. Secondly, the β-galactosidase and UDP-glucose lipid carrier transferase genes lacZ and wcaJ were deleted through the CRISPR/Cas9 system to block the decomposition of intermediate metabolites and explore the influence of these genes on 2'-FL synthesis. The fermentation results showed that single deletion of lacZ gene promoted the yield of 2'-FL by 1.88 times, which had no significant effect on cell growth. After double mutation of genes lacZ and wcaJ, the yield of 2'-FL increased by 4.89 times, and the cell growth was not affected significantly. Under optimized shake flask fermentation, the fermentation conditions were determined as follows: a defined medium was used, the induction concentration of IPTG was 0.2 mmol/L, the induction temperature was 25 ℃, and the inoculation amount was 5%. Under these conditions, the yield of 2'-FL reached 1.44 g/L. This study confirmed that deletion of β-galactosidase and UDP-glucose lipid carrier transferase genes could significantly increase the yield of 2'-FL, which provides a reference for expanding its industrial production.
  • SONG Ting, WANG Shuaijing, WANG Chen, LYU Yucai, LUO Huajun, GUO Jingling, GONG Dachun
    Food and Fermentation Industries. 2021, 47(3): 18-24. https://doi.org/10.13995/j.cnki.11-1802/ts.025002
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    Carbonyl reductases from Candida parapsilosis are valuable to the synthesis of hydroxyl compounds. By constructing recombinant E. coli BL21 (DE3)/pACYCDuet-1-cpcr, the carbonyl reductase CpCR gene (cpcr) from C. parapsilosis. ATCC 7330 was expressed. The recombinant CpCR was separated and purified by Ni-Agarose affinity chromatography from His-tag on the pACYCDuet-1 and its enzymatic properties were investigated. The results showed that the full length of the carbonyl reductase gene cpcr was 1 107 bp,containing 368 amino acids with molecular weight around 41 kDa and specific enzyme activity of 20 U/mg. The recombinant CpCR was stable in the temperature range of 4-33 ℃ with the relative enzyme activity above 80%, and the T50 value was 37 ℃. The suitable pH range was 6.2-7.5, and optimal stability was obtained under neutral conditions. Cu2+ had a strong inhibition on the recombinant CpCR, and lowered the relative enzyme activity by 30% at 1 mmol/L, and by 50% at a 5 mmol/L. The affinity of the enzyme to benzaldehyde and n-butyraldehyde was stronger than that to 4-chlor-3-keto- butyrate-ethyl ester (COBE). The catalytic efficiency for benzaldehyde or n-butyraldehyde was about 20 times of that for COBE. The recombinant CpCR was an NADPH-dependent carbonyl reductase. This study provided basic data for the molecular modification and catalytic application of carbonyl reductase CpCR.
  • YE Dexiao, HUANG Jiajun, LU Yujing, LIN Yucheng, LI Huiling, TAN Jinghang, ZHOU Jinlin
    Food and Fermentation Industries. 2021, 47(3): 25-30. https://doi.org/10.13995/j.cnki.11-1802/ts.025498
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    The α-L-rhamnosidase plays an important role in the conversion of natural products, and Pichia pastoris was used to express α-L-rhamnosidase AnRhaE. The pPIC9K-AnRhaE expression vector was constructed and transformed into Pichia pastoris GS115 and KM71H. After induced expression, SDS-PAGE electrophoresis showed that GS115 was more conducive to the production of AnRhaE. High AnRhaE activity was obtained at 45-55 ℃, and the optimum temperature was 55 ℃. AnRhaE was stable at pH 4.5-8, and the optimum pH was 5. AnRhaE could hydrolyze α-1,2, α-1,6 and rhamnosyl-rhamnosyl glycosidic bond, including naringin dihydrogen chalcone to trilobatin, naringin to prunin, rutin to isoquercetin, neohesperidin to hesperetin-7-O-glucoside, hesperidin to hesperetin-7-O-glucoside and epimedin C to icariin. AnRhaE had high activity to α-1,2 glycosidic bond than the α-1,6 glycosidic bond, and could complete hydrolysis epimedin C to icariin. Therefore, AnRhaE is potentially applicable in enzymatic conversion of natural products.
  • YU Jie, XU Qinqian, LI Ziyuan, LIU Hongyang, HAO Zaibin, LI Haiyun
    Food and Fermentation Industries. 2021, 47(3): 31-35. https://doi.org/10.13995/j.cnki.11-1802/ts.025689
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    Polydatin is abundant in the traditional Chinese medicine Polygonum cuspidatum, and the conversion of polydatin to resveratrol by biotransformation technology is an effective method to prepare natural resveratrol products. The cells of endophytic fungus Aspergillus aculeatus HZ001, which isolated from P. cuspidatum, can catalyze polydatin to resveratrol. The enzymatic characteristics of intracellular β-glucosidase produced by this strain were studied. The results showed that the optimum reaction temperature was 60 ℃, good stability was obtained below 60 ℃; and the optimum reaction pH was 4.8 with good stability at pH 3.6-4.0. The β-glucosidase could be activated by Fe2+ with a relative enzyme activity of 120% and significantly inhibited by Ca2+, Co2+, and Mg2+. SDS, mercaptoethanol, and dimethyl sulfoxide also inhibited the enzyme activity, and the highest inhibitory rate of 90% was obtained with EDTA. Under the optimal catalytic conditions, the kinetic parameters of the enzymatic reaction were established with the Km of 2.571 mmol/L and the Vmax of 0.594 μmol/(mL·h).
  • SHANG Xuejiao, FANG Sansheng, ZHU Yuanyuan, ZHAO Huijun, GUO Zhuang
    Food and Fermentation Industries. 2021, 47(3): 36-42. https://doi.org/10.13995/j.cnki.11-1802/ts.025193
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    As a characteristic fermented soybean product in Hubei, Hunan and Western Jiangxi, Meitauza is naturally fermented from soybean residue. However, there are few reports on its bacterial diversity. In this study, high throughput sequencing technology was used to analyze the bacterial diversity in Meitauza, and the gene function of bacterial community was further predicted by PICRUSt software. The results showed that the bacteria in the Meitauza were mainly subordinate to Proteobacteria (46.50%), Firmicutes (38.80%), Bacteroidetes (12.62%) and Actinobacteria (1.81%); the dominant bacteria genera were Acinetobacter (17.27%), Pseudomonas (10.38%), Brevundimonas (4.03%), Stenotrophomonas (3.05%), Comamonas (2.52%), Enterococcus (1.09%), Bacillus (11.60%), Brevibacillus (2.41%), Lysinibacillus (21.15%), Wautersiella (8.68%), Sphingobacterium (2.17%) and Nocardia (1.36%); eight operational taxonomic units(OTUs) were present in all samples of Meitauza, accounting for 0.59% of the total OTUs and 9.35% of the total sequences; 711 OTUs were only present in one Meitauza sample, accounting for 52.47% of the total OTUs and 6.34% of the total sequences, respectively. PICRUSt analysis revealed that bacterial groups in Meitauza had strong ability in transport and metabolism of carbohydrates and amino acids. Abundant microorganism communities were found in Meitauza and significant differences exist among bacterial communities from different samples.
  • ZHANG Meijuan, QIAN Pengzhi, DONG Liqing, HAN Yang, JI Guangxi, FENG Zhen
    Food and Fermentation Industries. 2021, 47(3): 43-48. https://doi.org/10.13995/j.cnki.11-1802/ts.026294
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    ZJ-01 strain isolated from contaminated fruiting body of oyster mushroom was used as the material,which was identified by morphological observation and homological analysis of ribosome 18S protein sequences,and the xylanase production conditions were optimized.The results showed that the morphology of ZJ-01 was similar to that of Trichoderma harzianum,and the phylogenetic tree analysis indicated that ZJ-01 strain had the closest genetic relation with T.harzianum BCS8A,then ZJ-01 strain was identified belonging to T.harzianum.The best carbon material and nitrogen material for xylanase production under solid-state fermentation of ZJ-01 were corn husk and corn alcohol distillate,respectively,and the most favorable mass ratio of nitrogen material and carbon material was 8∶2,the optimum fermentation time was 72 h.The xylanase activity was above 3 574 U/g under the optimum conditions.The results could lay foundation for further study of feasibility for producing xylo-oligosaccharide with xylanase.
  • CHEN Liang, WANG Yuqing, JI Feng, LI Yueqi, MA Yong, GU Ruizeng
    Food and Fermentation Industries. 2021, 47(3): 49-52. https://doi.org/10.13995/j.cnki.11-1802/ts.024969
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    The physical and chemical composition of corn oligopeptides was investigated in this study. Due to the unique amino acid sequence of corn protein which with high amount of glutamine and leucine and exist in adjacent positions, this study also focused on the pyroglutamyl-leucine (pyroGlu-Leu, pEL) dipeptide structure with antidepressant activity from corn oligopeptide. The results showed that corn oligopeptide had high protein content which reached 87.70%, and the peptide content was 82.76%. The average molecular weight was 461.20 Da and mainly in the range of 140-1 000 Da. The highest content of all amino acids was glutamic acid (glutamine) which reached 21.76%, followed by leucine with a content of 12.79% and leucine was the highest essential amino acid. The results of structural studies indicated that the content of pEL was more than 0.58% in corn oligopeptides, and it was stable in five batches of corn oligopeptide samples. This research provides a theoretical basis for developing corn oligopeptide as a new functional food.
  • XU Zhenzhen, YU Qiusheng, CHEN Tianxiang, PING Xiangli, ZENG Zhuqiang, CHENG Dan
    Food and Fermentation Industries. 2021, 47(3): 53-58. https://doi.org/10.13995/j.cnki.11-1802/ts.024745
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    An enzymatic hydrolysis of rice protein was studied by using alkaline protease and protease B in order to obtain rice protein peptides. And the process optimization was determined by single-enzyme- and double-enzyme-hydrolysis. The results showed that the protein recovery was as high as 43.9% and the peptides had high sensory score, low bitterness, and small molecular weight. Compared with the commercially available peptides from soybean protein and fish collagen, the moisture-, protein-, and fat-contents of the peptides from rice protein were similar. Moreover, the proportion of the peptides from rice protein and soybean protein less than 2000 Da were more than 80%, while the peptide from fish collagen was only 58.01%. The peptide from rice protein was the best in ACE inhibitory activity with the lowest IC50 of 4.94 g/mL. While it was 21.6% for soybean protein peptide and 14.1% for fish collagen peptide, respectively. Four rice protein peptide sequences were obtained by structural analysis, which were in line with the existing structure-activity analysis of ACE inhibitory activity. The ACE inhibitory activity of rice protein peptides produced in different batches showed that the ACE inhibitory rate and IC50 were basically stable.
  • LIN Nuoyi, CHENG Jian, WANG Qin, MA Lukai, LIANG Jiaxi, LI Sufen, YAO Wenqian, LIU Huifan
    Food and Fermentation Industries. 2021, 47(3): 59-65. https://doi.org/10.13995/j.cnki.11-1802/ts.024636
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    In order to make full use of pomelo resources and reduce environmental pollution, pomelo peel was used as the raw material and an improved Osborne method was used to extract proteins in stages. The structure of the protein was identified by scanning electron microscope, infrared spectrum and amino acid composition analysis. And the survival rate of mouse RAW 264.7 macrophages, NO content, and the expression level of IL-6 and TNF-α were detected by MTT, and ELISA detection, respectively. It was found that the extraction rates of albumin, globulin, gliadin, and glutenin were (15.47±0.17)%, (4.20±0.09)%, (0.18±0.02)% and (0.04±0.01)%, respectively. The results of scanning electron microscope showed that the albumin was in the form of a sheet and the globulin was in a porous form, while the gliadin was in a strip-like structure. Moreover, the conformations of the three proteins only changed at the functional groups of N—H, —OH, and —CH. Furthermore, the content of glycine and alanine were the most abundant which reached 125.49 g/100 g and 50.48 g/100 g respectively. Albumin had the strongest ability to stimulate RAW 264.7 cells to produce NO and TNF-α and IL-6 at 800 μg/mL, at 95.46 μmol/L, 9 528.05 pg/mL and 1 769.33 pg/mL, respectively. The experimental data showed that pomelo peel albumin had the strongest immune activity, suggesting its potential as a health food development and medical drug.
  • LIU Shuang, ZHANG Qian, DU Chuan, ZHOU Tao, ZHAO Jiahao, SHI Lei, WANG Chunling
    Food and Fermentation Industries. 2021, 47(3): 66-71. https://doi.org/10.13995/j.cnki.11-1802/ts.025374
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    To study the safety of traditional soy sauce fermentation strain Zygosaccharomyces rouxii, GC-MS was used to explore the metabolites of strains in fermentation mash to ensure the metabolic safety of traditional strains; bile salts hydroxylase activity detection, hemolysis test and drug resistance test were used to evaluate the safety of the strain. The results showed that no harmful metabolites were detected and the sugars and amino acids were converted into alcohol and ester substances during fermentation. Z.rouxii did not produce bile salt hydroxylase or hemolytic strains. Z.rouxii was sensitive to antibiotics (voriconazole, ketoconazole, nystatin, and amphotericin B) and showed a dose-dependent effect to fluconazole. The minimum inhibitory concentration (MIC) of amphotericin B and fluconazole against Z.rouxii was 2 μg/mL and 8 μg/mL, respectively, which were lower than the drug resistance standard of US National Committee for Clinical Laboratory. This study laid a foundation for further understanding of the safety of Z.rouxii, and also provided a theoretical basis for the safety of traditional strains.
  • LU Juan, XIE Dongxue, HE Liuyang, WANG Yue, ZHENG Zhiyan
    Food and Fermentation Industries. 2021, 47(3): 72-78. https://doi.org/10.13995/j.cnki.11-1802/ts.024738
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    In this study, polysaccharides from Matricaria chamomilla, extracted by reflux were comprehensively investigated. Two homogenous polysaccharides (MCP-1-1 and MCP-2-1) were obtained through DEAE-Sepharose and Sephadex G-100 chromatography. The total polysaccharide contents of MCP-1-1 and MCP-2-1 were 82.23% and 84.09% respectivley. Moreover, the contents of uronic acid were 4.11% and 5.38% respectively. And the protein contents were 0.58% and 1.05%. Besides, the solubility of MCP-1-1 and MCP-2-1 were 98.36% and 97.44%. Furthermore, MCP-1-1 was composed of rhamnose, glucuronic acid, galactose, glucose, xylose and arabinose, meanwhile MCP-2-1 was composed of glucuronic acid, galactose, glucose, galacturonic acid, xylose and arabinose. The molecular weight of MCP-1-1 and MCP-2-1were 30 902 and 7 244 Da. Further study indicated that two polysaccharides had pyran ring and β- type glycosidic linkages and had no triple- helix structure, showed excellent thermal stability. Two homogenous polysaccharides had scavenging effect on both DPPH and hydroxyl free radical, and the scavenging ability was positively correlated with the concentration of homogeneous polysaccharides, the scavenging ability of MCP-2-1 was significantly higher than that of MCP-1-1. The research results revealed that MCP-1-1 and MCP-2-1 could be used as natural antioxidant in food and cosmetics industry, and this study could provide theoretical support for the development and utilization of chamomile polysaccharides.
  • LIU Yanxia, ZHANG Liping, ZHAO Gaiming, ZHU Chaozhi, LI Miaoyun, BEI Cuiping, CAN Muyou, ZHANG Jicai
    Food and Fermentation Industries. 2021, 47(3): 79-86. https://doi.org/10.13995/j.cnki.11-1802/ts.023327
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    In order to investigate the tenderness of Simmental hybrid cattle and Qinghai plateau yak, the rump, shoulder, silverside, topside, knuckle meat were used for the production of beef jerky respectively. And the shear force, water content, water activity, moisture migration patterns, scanning electron microscopy (SEM) were measured as index to explore their tenderness. The results showed that the shear force of beef jerky and yak jerky was the smallest, and the moisture content and water activity of yak jerky were significantly higher than that of other parts. Besides, the shoulder meat had higher moisture content and moisture activity. The beef jerky had better taste and was suitable for industrial production. Yak jerky made from topside had higher taste score than other parts. By combining correlation and linear regression, it was concluded that R2 was the highest in the equation y=0.984x+13.911 for the meat tenderness of Qinghai plateau yaks. Moreover, R2 was the highest in the equation y=-0.993x+14.234 for the meat tenderness of Simmental hybrid topside. And the principal component analysis (PCA) comprehensive evaluation model could be used to process topside into beef jerky and shoulder part into yak jerky.
  • WANG Cuntang, GAO Zengming, ZHANG Fujuan, ZHU Hongfei, ZHOU Qingwen, KONG Baohua
    Food and Fermentation Industries. 2021, 47(3): 87-94. https://doi.org/10.13995/j.cnki.11-1802/ts.024984
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    The effect of ethanol extract of onion skins (EEOS) on color browning, lipid and protein oxidative as well as infection degree of microorganisms of fresh pork was studied, and was compared with control (without any antioxidant) and ascorbic acid (VC). The objective was to determine the effect of color browning by brightness (L*), redness (a*), and the content of methemoglobin (MetMb). The inhibitory effect of EEOS on the oxidation of pork lipid and protein was studied based on thiobarbituric acid reacting substances (TBARS), hydrophobicity and carbonyl content of protein in samples at different time points. Total volatile basic nitrogen (TVB-N) content was used to characterize the infection degree of microorganisms to meat quality. Results showed that EEOS could reduce MetMb content and increase a* value of meat samples. On the 12th day, TBARS and carbonyl content of 2.0% EEOS treatment were decreased by 53.1% and 29.2% respectively than control, which were lower than those in VC treatments. In addition, TVB-N values and protein hydrophobicity of each EEOS treatment were lower than control, and showed a concentration dependent relationship (P<0.05). These results indicated that EEOS has a good research prospect as a natural antioxidant or preservative.
  • HAO Jingxiao, SHI Fulei, HUI Jingru, CHENG Yangyang, HUANG Zhanwang
    Food and Fermentation Industries. 2021, 47(3): 95-100. https://doi.org/10.13995/j.cnki.11-1802/ts.025078
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    In order to study the processing adaptability of Agrocybe cylindracea powder and explore the more suitable crushing method, three different varieties of A.cylindracea were used as raw materials in this experiment. First, the coarse powder of A.cylindracea was obtained through common grinding, and then the coarse powder was made into ultrafine powder through ultrafine grinding. And the processing physical characteristics of A.cylindracea coarse powder and ultrafine powder were compared. The results showed that the average value of the median diameter of coarse powder was 84.03 μm, while the average value of ultrafine powder was 24.57 μm. Compared with coarse powder, the particle size of ultrafine powder was significantly reduced. Moreover, with the reduction of particle size, the scanning electron microscope results revealed that the powder surface changed from a lamellar structure to a fluffy structure. Besides, the bulk density became smaller, and the filling ability was reduced. The slip angle and angle of repose became smaller, and the fluidity increased. Furthermore, the water holding capacity and expansion force became smaller and the hydration capacity was reduced. The water solubility index of coarse powder and ultrafine powder both rose first and then decreased and then tends to be gentle as the temperature increased. The water solubility index was highest at 60 ℃, and the water solubility index of ultrafine powder was greater than that of coarse powder. FT-IR analysis showed that there was no significant change in the functional group structure of A.cylindracea powder after ultrafine grinding. Above research shows that the Agrocybe aegerita ultra-fine powder has broad application prospects. Besides, the results of this study provide a theoretical basis for the further processing of A.cylindracea.
  • HUANG Di, LI Cuiyun, WANG Minhui, CHENG Qin, LUO Xiangyuan, ZHONG Jiliang, YE Jinsong
    Food and Fermentation Industries. 2021, 47(3): 101-106. https://doi.org/10.13995/j.cnki.11-1802/ts.024786
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    Transglutaminase (TGase) can effectively improve the properties of proteins through cross-linking, therefore improves the properties of foods. Egg yolk powder was obtained by spray drying with different amounts of TGase in egg yolk. The emulsifying and gelling properties of egg yolk powder were studied. The secondary structure of protein in egg yolk powder was determined by Fourier transform infrared spectroscopy (FTIR) technique to analyze the influence of enzymes on the emulsifying properties of yolk powder. The effect of TGase on the gelling function of egg yolk powder was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE)electrophoresis and determination of superficial thiol number. The results showed that with the addition of TGase, the gelling of egg yolk powder initially increased and then decreased. The gelling was maximized when the additive amount of TGase was 1.5 U/g egg yolk. According to superficial thiol number analysis, the cross-linking effect of TGase could change the superficial thiol number, hence change the gelling network state, so that the water holding capacity of the egg yolk powder was changed and its gelling properties improved,. The emulsification activity index (EAI) of egg yolk powder first increased and then decreased with the additive amount of TGase, and the highest EAI was obtained with 2.0 U/g TGase. According to FTIR, the α-helix changed to β-sheet, with the same trend as EAI. The emulsion stability index (ESI) of egg yolk powder first decreased and then increased with the additive amount of TGase, and the lowest ESI was obtained with 2.0 U/g TGase.
  • ZHANG Minqian, LIU Gongliang, FEI Yongtao, BAI Weidong, AI Lianzhong, YU Jianshen
    Food and Fermentation Industries. 2021, 47(3): 107-113. https://doi.org/10.13995/j.cnki.11-1802/ts.024655
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    At present, research on the synthesis of trehalose with food grade microorganisms has attracted much attention. Since Zygosaccharomyces mellis LGL-2 can tolerate high glucose concentration and synthesize trehalose through stress metabolism, the effects of fermentation time, inoculation amount, culture temperature, initial concentration of glucose and rotational speed on trehalose production by LGL-2 were studied. The optimal fermentation conditions were determined by response surface methodology. The optimal conditions were determined as follows: 96 h, 10% inoculation size, 300 g/L initial glucose concentration, 24 ℃ and 180 r/min. Under the optimized conditions, the yield of trehalose was 92.32 mg/g dry yeast, which was 29.34% higher than that before optimization. The results provide reference for the application of Z. mellis and the biosynthesis of trehalose.
  • LIN Bingjie, XUE Peng, JING Jinjin, ZHANG Ruoyu, JI Xiaoying, HAN Caijing, ZHANG Fengxiang
    Food and Fermentation Industries. 2021, 47(3): 114-119. https://doi.org/10.13995/j.cnki.11-1802/ts.025441
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    Quinoa husks protein was extracted by high concentration ethanol from quinoa husks, and the protein was hydrolyzed by four kinds of proteases (alkaline, neutral, compound and flavor protease) to produce polypeptides. The molecular weight distribution and amino acid composition of quinoa protein, the enzymatic hydrolysis ability of four proteases and the activities of the polypeptides were determined. The results showed that quinoa husks protein with molecular weight of 22.8 kDa, 39.1 kDa and 52.7 kDa contained 17 kinds of amino acids, and the ratios of essential amino acids and hydrophobic amino acids to total amino acids were 35.17% and 28.48%, respectively. The hydrolysis ability of alkaline protease for quinoa husks protein was the highest, with degree of hydrolysis reached 13% at 120 min, and the peptides yield was 88.88%. The peptides produced by flavor protease showed high α-glucosidase inhibitory activity and Fe2+ chelating ability, which were 81.67% and 89.03% respectively; the inhibition rate of alkaline protease produced peptides against tyrosinase was 73.17%, while neutral protease produced peptides had strong scavenging ability to DPPH free radicals, which was 84.91%. This study showed that quinoa peptides had good biological activities, which could provide a theoretical basis for further development and utilization of quinoa husks.
  • LIAO Ruoyu, ZHANG Chune, LIU Xinbao, SUN Yue, TIAN Jianwen
    Food and Fermentation Industries. 2021, 47(3): 120-127. https://doi.org/10.13995/j.cnki.11-1802/ts.024941
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    This paper established a method for the extraction and determination of bright blue in black rice. Sodium bicarbonate was used as an auxiliary extraction reagent. The sample was firstly extracted with 40% methanol-water solution. Then after the centrifugation, it was further purified with a solid phase extraction column Strata-X-AW and followed by analysis using HPLC. It was verified that, with the addition of 0.52 g sodium bicarbonate and 40% methanol-water as extraction solvent, then vortex treatment for 12 minutes with three repetitions, the bright blue added in the black rice could be completely extracted. The results showed that the quantification method had a good linear relationship (R≥0.999 9), with the detection limit of 0.02 mg/kg and the qualification limit of 0.05 mg/kg. The recovery rate of standard addition was between 81.70% and 91.52%, and the relative standard deviation (RSD) was between 0.39 % and 1.75%. Compared with the method in GB 5009.35—2016, the current method is more convenient, quicker, simpler and more accurate. The improved extraction method is more suitable for the rapid determination of bright blue in black rice and cereal flour products, which are easy to swell in water.
  • LI Qi, ZENG Fankun, HUA Rong, WANG Jifei
    Food and Fermentation Industries. 2021, 47(3): 128-134. https://doi.org/10.13995/j.cnki.11-1802/ts.025097
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    The insoluble dietary fiber (IDF) was extracted from chive root by ultrasound-assisted treatment, and the effects of solid-liquid ratio, ultrasonic temperature, ultrasonic power intensity and ultrasonic time on IDF yield were discussed. Then the Box-Behnken central combination principle was used to establish a mathematical model and the parameters of extraction was optimized by response surface design. Finally, the physical and chemical property detection, infrared spectrum analysis, thermogravimetric analysis and scanning electron microscope observation of IDF from chive root were explored. The results showed that the best extraction process was as follows: the ratio of liquid to material was 25 mL/g and the ultrasonic temperature at 56 ℃ for 48 min with 60% of ultrasonic power intensity. So the yield reached 32.92% under the optimal condition, and the relative error was 2.80% compared with the theoretical value of 33.87%. Moreover, the water holding capacity, swelling capacity and oil holding capacity of IDF from chive root were 11.90 g/g, 8.23 mL/g and 7.63 g/g, respectively. With good stability and typical network structure, the IDF from chive root has the typical dietary fiber properties. These results showed that chive root was a good source of high-quality dietary fiber, and it's effective to us response surface optimization to improve the yield of IDF. This study provides basis for the extraction, improvement, and application of IDF from chive root.
  • XIE Sandu, CHEN Huiqing, ZHUANG Peirong, HONG Jiarong, YOU Lijie
    Food and Fermentation Industries. 2021, 47(3): 135-142. https://doi.org/10.13995/j.cnki.11-1802/ts.025099
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    The portable Ganoderma lucidum and white tea which was made from white tea, white Ganoderma, Coriolus versicolor and black Ganoderma to explore the effect of different brewing time and different brewing times on anti-oxidation activity of the tea infusion. And in which tea polyphenol was used as positive control and the elimination rates of hydroxyl radical, DPPH, superoxide free radical and inhibitory rate on Fe2+-induced lipid peroxidation of vitellin were used as index. The results showed that the optimum composition of the portable Tea bags (3.75 g/bag) was: white tea 2.5 g, white Ganoderma 0.4 g, Coriolus versicolor 0.55 g, black Ganoderma 0.3 g. Moreover, immersion time, the times of immersion and their interaction influenced the anti-oxidation activity of the tea infusion were extremely significantly (P<0.01). When it was treated by 3.75 g of the portable Ganoderma lucidum and white tea in hot water (200 mL water, temperature>95℃, 3 min) and removing tea residue, the anti-oxidation activity of the tea infusion reached its peak value (P<0.05). Moreover, the tea infusion demonstrated an elimination (74.05±0.82)% of the hydroxyl free radical, (55.86±2.23)% superoxide free radical, which were equal to that of 0.45 mg/mL, 0.40 mg/mL tea polyphenol(P>0.05)respectively. And the radical scavenging rates of DPPH reached (63.07±2.37)%, which was higher than that of 0.45mg/mL tea polyphenol (P<0.05). Furthermore, the inhibitory rate of Fe2+-induced lipid peroxidation of vitellin reached (60.66±2.94)%, which was higher than that of 0.70 mg/mL tea polyphenol (P<0.05). Therefore, the brewing Ganoderma lucidum and white tea infusion had strong antioxidant activity.
  • SHAO Jianhui, MENG Feishuai, WANG Miao, ZHU Yifan, BAI Zhongbin, MA Chunhua
    Food and Fermentation Industries. 2021, 47(3): 143-149. https://doi.org/10.13995/j.cnki.11-1802/ts.024980
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    To explore the oenological potential of grapevine variety ‘Fa-guoye' from Yunnan, and recommend a best winemaking category and process, different pre-fermentation treatments were compared to make red and rosé wines. Skin maceration-fermentation (MF) and CMS-MF were used to make red wine, while cold pre-fermentation maceration and saignée (CMS) and direct press (DP) for rosé wine. Malolactic fermentation (MLF) was carried out in red winemaking after alcohol fermentation. After three-months aging, physical and chemical analysis were carried out as well as sensory evaluation. The results showed that CMS and CMS-MF significantly enhanced the extraction of tannin, anthocyanin, total extract and color of rosé and red wines compared with DP and MF separately. The aroma, taste and overall evaluation scores of rosé wines from CMS and DP treatments were significantly higher than those red wines from MF and CMS-MF. However, no statistically significant difference was found in the sensory quality of the same wine category (P<0.05). And the overall evaluation scores had significant positive correlations with alcohol and titratable acid contents, and significant negative correlations with anthocyanin, tannin, residual sugar and volatile acid contents. Therefore, both MF and MLF had adverse effects on the sensory quality of ‘Fa-guoye' wine. Compared with CMS, DP led to significant increases in reducing sugar of grape juice and alcohol content of the final wine, and significantly decreased tannin contents while maintaining the same sensory quality of rosé wine. Therefore, ‘Fa-guoye' grape is suggested to make quality rosé wine by DP meanwhile reducing the process and saving costs.
  • DAI Jingjing, LYU Kui, SHI Zhongping
    Food and Fermentation Industries. 2021, 47(3): 150-156. https://doi.org/10.13995/j.cnki.11-1802/ts.024716
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    In recent years, the shortcomings of traditional operation mode in fermentation industry have been gradually emerged, including the low level of automation, outdated management mode and low utilization of fermentation statistics. To solve these problems in actual fermentation process, the PC terminal of BioJN fermentation technology service system was designed and developed with Python 3.6. This PC terminal has a simple operation interface and a full-featured data visualization module. A complete database system for data storage, retrieval and management was built by MySQL, and the communication module could collect data through reserved communication interfaces, including dynamic data exchange (DDE), OLE for process control data access (OPC DA) and OPC unified architecture (OPC UA). The PC terminal can upload the local collected data to the server, which can be used together with the server to achieve the functions of user authority management, fermentation data management, remote monitoring and control of fermenters. The PC terminal was connected to different bioreactors for multiple fermentation experiments to fully validate its effectiveness and versatility. The results indicated that all function modules this system worked stably as expected. All function modules in this PC terminal have been running stably as expected.
  • LI Shuhui, LIU Wei, GUO Ting, ZHOU Hongyuan, ZHANG Yuhao, LIU Xiaozhu, MA Liang
    Food and Fermentation Industries. 2021, 47(3): 157-163. https://doi.org/10.13995/j.cnki.11-1802/ts.025087
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    In this paper, an electrochemical sensor technology based on brush-shaped nano-ZnO enrichment and aptamer specific recognition was established for high-sensitivity detection of patulin (PAT) in fruits. It was introduced to increase the load of PAT-specific aptamers, thereby enhancing the identification and capture capabilities of this sensing system for the target PAT, and realizing high-sensitivity detection of PAT. Results showed that the linear range of this method was 0.5-1×103 μg/L with the detection limit of 0.15 μg/L. And the average recovery of spiked apple juice and hawthorn tablets was between 94.75% and 110.84 % which has a good anti-interference ability for ochratoxin A. This method combined with the controllable morphology nanomaterials enriched the biorecognition ability of PAT aptamers, thereby could realize the rapid, accurate and high sensitivity detection of PAT, and provide a new idea for the construction of other mycotoxin sensors.
  • ZHANG Yuanyuan, MENG ZhenQIU Kai, DONG Siyuan, WU Zhuying, GUO Xingguang, ZHONG Qiding
    Food and Fermentation Industries. 2021, 47(3): 164-169. https://doi.org/10.13995/j.cnki.11-1802/ts.024477
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    A species-specific PCR identification method was established to identify five kinds of animal-derived components of pork, beef, mutton, chicken and duck in long-term high-temperature processed foods such as canned meat. The total DNA templates of five kinds of animals were amplified by PCR. Then the amplified products of 212 bp, 147 bp, 202 bp, 131 bp, and 201 bp were obtained. Moreover, the sequencing results were verified by using the BLAST comparison on NCBI to confirm the accuracy of the experiment, and the commercially available canned samples were also been tested. In this method, the five animal introduced species have good specificity, the detection sensitivity for derived components of pork, beef, mutton and duck could reach 1%, and the detection sensitivity for chicken-derived components was 2.5%. After testing the commercially available samples, it was clear that in 6.6% of the samples, the actual composition did not match the label. This method was easy to operate, low in cost, accurate, reliable, which could be widely used in the identification of five animal-derived components of pigs, cattle, sheep, chickens and ducks in canned meat and long-term high-temperature processed foods. Above all, it had extensive value in applications.
  • JU Xiaojun, SHAN Yanju, LIU Yifan, JI Gaige, ZHANG Ming, TU Yunjie, ZOU Jianmin, SHU Jingting
    Food and Fermentation Industries. 2021, 47(3): 170-175. https://doi.org/10.13995/j.cnki.11-1802/ts.025140
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    This paper analyzed the volatile organic compounds in the muscles of broiler breeds with different growth rates based on GC-IMS. The broiler breeds with different growth speeds were selected as slow-speed chickens (Langshan chicken), medium-speed chickens (3A, Huashan Ma chickens), and fast-type chickens (Rose 308). There were six broilers of each breed. GC-IMS measurement was performed on the same part of the pectoral muscle. The research results showed that 85 kinds of volatile organic compounds and 29 kinds of qualitative substances have been detected, mainly including aldehydes, ketones, alcohols and other compounds by GC-IMS. The content of aldehydes and alcohols in Langshan chicken of the slow-type chicken was significantly higher than that of the other three types (P<0.05).The main characteristic substances including trans-2-octenal, valeraldehyde, nonanal, hexanal, 1-butanol, ethanol, 1-pentanol, 1-octen-3-ol. The characteristics of 3A and Huashan of the medium-speed chicken were similar, the content of 3-hydroxy-2-butanone and ethyl acetate was extremely high. Moreover, rose 308 of the fast-type chicken had a low content of most substances, and the main characteristic substance was 3-pentanone. In summary, GC-IMS could analyze the difference in flavor of chicken meat quality at different growth rates. GC-IMS could intuitively and quickly detect the specificity and content difference and similarity of volatile organic compounds of broiler breeds with different growth speeds, and could clearly distinguish broiler breeds with different growth speeds.
  • CHEN Weifeng, CHEN Wei, CAI Ying, CUI Liwei, HAO Xiuzhen, WANG Xiaohong
    Food and Fermentation Industries. 2021, 47(3): 176-180. https://doi.org/10.13995/j.cnki.11-1802/ts.025010
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    A method for rapid detection of Listeria monocytogenes was established using color reaction of H2O2-oxidized 3,3',5,5'-tetramethylbenzidine (TMB) catalyzed by gold nano-enzyme, combined with high specific and affiliative aptamer. Under optimized experimental conditions, the linear regression equation describing the relation between concentration of L. monocytogenes and absorbance at a specific wavelength was determined and expressed as y=0.032 1x+0.024 8 (R2=0.996 3). The detection limit was calculated as 5 CFU/mL, the sample recovery rate was 93.5%-105.4%, and the relative standard deviation was less than 10%. In summary, the method has the advantage of strong specificity, simple operation, and low cost, and can be used for rapid quantification of L. monocytogenes in foods.
  • WEI Jie, YIN Yajie, JIANG Wei, SONG Cengye, WEI Ling, LIAO Zhili, HAN Xinglin
    Food and Fermentation Industries. 2021, 47(3): 181-187. https://doi.org/10.13995/j.cnki.11-1802/ts.024783
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    Bamboo leaves and bamboo leaf extracts were widely used in Chinese baijiu, Chinese yellow rice wine and health drinks. The volatile substances of seven kinds of bamboo leaves were studied based on head space solid phase microextraction (HS-SPME), gas chromatography mass spectrometry (GC-MS) and statistical analysis in order to support theoretical foundation for the flavor of bamboo liquor. One hundred and forty two volatile substances were accurately identified from seven kinds of bamboo leaves by the index retrieval and retention index validation which including esters, alcohols, aromatic compounds, aldehydes, ketones, heterocycles, acids, ethers and terpenes. And among which nine substances such as hexanal, 2-hexenal and methyl heptene were common volatile components in seven kinds of bamboo leaves. According to the classified statistics of seven kinds of bamboo leaf compounds, it was found that cis-2-hexenal was the main aldehydes and the relative content range from 26.14% to 62.26%. The content of methyl decanoate (23.84%-49.96%) in esters and lactones were relatively higher, and the content of α-ionone in ketones was higher which between 0% and 53.33%. The phenyl ethanol was the main compound of alcohols, ranged from 18.49% to 75.65%. Moreover, terpenoids were the main components of bamboo leaves, and the relative content of linalool (39.12%-100%) was the highest. Three principal components were extracted by PCA (the contribution rate was 73.52%), in which esters and lactones and aromatic substances were closer on the PC1 and made a greater positive contribution to PC1. The cluster analysis showed that Dendrocalamus membranceus Munro were quite different from with the other six varieties, and the flavors of Fargesia spathacea Franch, Pleioblastus amarus and Phyllostachys bambusoides f. lacrima-deae bamboo leaves were similar, meanwhile, endrocalamus latiflorus Munro, Lingnania intermedia and Pleioblastus amarus leaves had little difference.
  • JIN Youlan, HUANG Tian, JIANG Ronggang, HUANG Fangfang, LIU Zhonghua, HUANG Jianan, LI Qin
    Food and Fermentation Industries. 2021, 47(3): 188-196. https://doi.org/10.13995/j.cnki.11-1802/ts.025075
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    This research adopted sensory evaluation,solid phase microextraction/gas chromatography-mass spectrometry (GC-MS),odor activity value (OAV) method and chemometrics to analyze the characteristic aroma components of Fu-brick tea, fungal fermentation white brick tea and fungal fermentation black brick tea. The results showed that the three kinds of brick tea had their typical “fungus aroma”, but Fu-brick tea were fungus aroma with woody aroma, slightly muddy, fungal fermentation white brick tea were fungus aroma with medicinal aroma, fungal fermentation black brick tea were fungus aroma with floral aroma. Solid-phase microextraction-gas chromatography-mass spectrometry determined 75 volatile components in three fermentation brick tea samples,among which the main components were aldehydes, alcohols, ketones and hydrocarbons, with 27 kinds of volatile fragrant components. Based on OAV calculation and partial least squares discriminant analysis (OPLS -DA), 15 key different aroma components were identified. Moreover, Fu-brick tea had hexanal, nonanal, (E,E)-2,4-nonadienal, (E,E)-2,4-heptadienal, 2-n-pentylfuran, 3,5-octadien-2-one, methylheptenone, β-ionone and linalool oxide I with green and woody properties as the characteristic aroma component. Furthermore, fungal fermentation white brick tea had phenylacetaldehyde with green properties as the characteristic aroma component. Fungal fermentation black brick tea had 1-octen-3-ol with mushroom fragrance and earthy properties, cinnamyl alcohol, linalool and methyl salicylate with floral properties, cedar alcohol with sweet woody properties as the characteristic aroma component.
  • HU Jiayi, MOU Fangting, SHI Lilin, HUA Rong, WANG Jifei, ZENG Fankun
    Food and Fermentation Industries. 2021, 47(3): 197-204. https://doi.org/10.13995/j.cnki.11-1802/ts.025522
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    To explore the differences in nutrition and aroma components and the influences of seasons on hotbed chives and leeks in Puding county, the nutritional components were determined by national standard methods and the aroma components were determined by GC-MS. The results showed that there were significant differences in nutritional components between hotbed chives and leeks (P<0.05), both of which contained two principal components and their water content was as high as 90%. The contents of crude protein, total sugar, crude fiber and polyphenol were the highest in summer leeks, while the contents of crude fat, titrable acid and Vc were the highest in winter. The basic nutrition of hotbed chives was lower than that of leeks, and the contents of crude protein, total sugar, Vc and polyphenol were higher in summer. However, the contents of crude fat, titratable acid and crude fiber were slightly lower than that in winter. Besides, leeks had a higher amino acid value, but a lower TAV of savory and sweet amino acids. Moreover, the aroma components of hotbed chives were divided into three categories, and leeks were divided into two categories. There were 27 and 24 kinds of aroma components could be detected in summer Hotbed chives and leeks respectively, while in winter detected 16 and 18 kinds respectively with the main aroma component of organic sulfur compounds. The contents of allyl methyl disulfide (413.23-661.70 ng/g) and dimethyl trisulfide (478.41-726.30 ng/g) in hotbed chives were the highest, while the contents of allyl methyl sulfide (793.40-925.08 ng/g) and allyl methyl disulfide (785.28-837.60 ng/g) were the highest in leeks. In short, the nutritional value of winter leeks is higher, while the flavor of hotbed chives is better, which is bigger affected by seasons and beneficial to the seasonal development of resources.
  • XU Zheng, LIU Yazhi, PAN Senhao, LI Guangyao
    Food and Fermentation Industries. 2021, 47(3): 205-210. https://doi.org/10.13995/j.cnki.11-1802/ts.025128
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    L-Xylulose is a rarely L-form pentose, and is able to inhibit the activities of glycosidases for the purpose of controlling human blood sugar levels. It is also useful as a pharmaceutical intermediate. Currently, L-xylulose can only be obtained by artificial synthesis, such as enzymatic catalysis targeting xylitol or L-arabinitol by oxidoreductases. In recent years, bio-production procedure of L-xylulose has been extensively studied. This review focuses on investigations of key enzymes for L-xylulose production, as well as the production processes, to provide theoretical references for relative research in this field.
  • JIA Dongxu, SUN Chenyi, PENG Chen, LIU Zhiqiang, LI Mian, WANG Hongyan, CHEN Kaixi, CHENG Xinping, CHEN Deshui
    Food and Fermentation Industries. 2021, 47(3): 211-217. https://doi.org/10.13995/j.cnki.11-1802/ts.025236
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    D-psicose is a kind of hexoketose with specific health care function, which is usually applied to prevent diabetes, maintain normal blood lipid level and avoid the negative effect of traditional sweeteners on human body. Thus, it shows important application value. In recent years, a variety of methods regarding synthesis of D-psicose have emerged with the aim to solve the problem of its rare content in nature. In this paper, the aspects including physiological function, chemical synthesis and enzymatic isomerization process of D-psicose were reviewed. Among them, enzymatic resources and properties, crystal structure and corresponding catalytic mechanism, molecular modification, multi enzyme co-expression, expression in food grade hosts are systematically elucidated. This review may shed light on improving the industrial preparation level of D-psicose via biotransformation method and provide new ideas for maintaining public nutrition and health care.
  • BAO Yi, HU Youming, ZHU Linjiang, LU Yuele, CHEN Xiaolong
    Food and Fermentation Industries. 2021, 47(3): 218-223. https://doi.org/10.13995/j.cnki.11-1802/ts.025204
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    Hexose oxidase (HOX) has a wide substrate spectrum, which makes it quite different from glucose oxidase. HOX catalyzes the oxidation of mono- and disaccharides to their corresponding lactones, including D-glucose, D-fructose, D-galactose, maltose, and lactose, with concomitant reduction of molecular oxygen to hydrogen peroxide. In recent years, HOX had been shown to have great application potential as a novel green additive in food, feeding and other industries due to its function of deoxygenation, acidification and improvement of protein cross-linking. HOX is primarily derived from red algae species. Current production of HOX is mainly dependent upon the isolation from the natural source. Studies have shown active expression and fermentation production in genetic engineered yeast. This review focused on the separation, purification, enzymatic properties, and heterologous expression of HOX, as well as some application cases, as to provide references for its development and application.
  • HU Guanhua, WANG Debao, SU Lin, ZHAO Lihua, TIAN Jianjun, ZHANG Zhonghai, ZHU Haotian, JIN Ye
    Food and Fermentation Industries. 2021, 47(3): 224-229. https://doi.org/10.13995/j.cnki.11-1802/ts.024344
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    Food-derived chelating peptides have chelating activity on mineral elements and can improve the bioavailability of mineral elements. Peptides with calcium binding activity can effectively transport calcium ions through intestinal epithelial cells and then be absorbed by body. The preparation, isolation, purification, structure characteristics, physiological activity and bioavailability of food-derived calcium chelating peptides were summarized to provide reference for research and development of calcium supplement products and for efficient, high-value and sustainable development and utilization of the calcium resources in China.
  • FENG Lin, CHEN Xuelan
    Food and Fermentation Industries. 2021, 47(3): 230-236. https://doi.org/10.13995/j.cnki.11-1802/ts.024842
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    Mycotoxins are toxic secondary metabolites produced by some fungi during growth and reproduction. They have carcinogenic, teratogenic and mutagenic effects, which seriously endanger human health. Therefore, their detection and control are of great importance. However, the existing detection methods generally have the disadvantages of complex sample pretreatment, high cost and cumbersome operation, and may cause potential secondary hazards to operators and the environment. The detection sensitivity of mycotoxins can be improved with new nanomaterials, and the mimic epitopes of mycotoxins and the recombinant antibodies against mycotoxins can be screened through phage display technology, hence reducing the detection cost and achieving the purpose of non-toxic detection. The recent application of different nanomaterials and phage display technology and their combination in mycotoxin detection were reviewed, and the future development is prospected.
  • FANG Jingyu, XIE Hualing, FENG Simin, LI Zhenhao, LI Mingyan, XU Jing, SHAO Ping
    Food and Fermentation Industries. 2021, 47(3): 237-244. https://doi.org/10.13995/j.cnki.11-1802/ts.024475
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    Dendrobium is currently been widely used in supplements and precious foods. It contains a variety of bioactive ingredients such as polysaccharides, alkaloids, amino acids and trace elements. Due to the high incidence of metabolic-related diseases, the bioactivities of Dendrobium in the prevention and treatment of diabetes have been gradually tapped, and also received more and more attention. Modern pharmacological studies have confirmed that Dendrobium polysaccharide, as the main bioactive ingredient in Dendrobium, had a significant effect on lowering blood sugar, and could improve the complications of diabetes. And thus had the potential to be developed as a health food and medicine. This article described the pharmacological effects of Dendrobium polysaccharides in diabetes (lowering blood sugar, preventing and treating diabetes complications) and summarized the factors and main mechanisms about the pharmacological activity of Dendrobium polysaccharides. So it provides guide for the research and application in the prevention and treatment of diabetes.
  • JU Ning, GOU Meng, ZHANG Tongtong
    Food and Fermentation Industries. 2021, 47(3): 245-251. https://doi.org/10.13995/j.cnki.11-1802/ts.024032
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    Proteomics technology is an important tool for life science research. It has unique advantages in the fields of medicine, animal and plant research. Proteomics technology can further clarify the expression and their dynamic changes of proteins in milk and dairy products and which has become a research hotspot currently. This paper summarizes the concept, common methods and application fields of proteomics. The application of proteomics in the field of milk and dairy products are introduced emphatically, especially in milk fat globule membrane proteins, whey proteins, milk and dairy product processing, and cheese. And the current problems and limitations are also been discussed. The prospect of proteomics, as well as its application in milk and dairy products, are forecasted which provide a theoretical basis for the in-depth study of milk and dairy products by proteomics technology.
  • TIAN Jie, ZHANG Chuanbo, SUN Yunzi
    Food and Fermentation Industries. 2021, 47(3): 252-258. https://doi.org/10.13995/j.cnki.11-1802/ts.024835
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    Flavonoid is a kind of compound whose parent structure is 2-phenyl chromone, and its basic carbon skeleton is C6-C3-C6. There are many kinds of flavonoids, most of which have biological activity. They are widely used in many fields, such as food and health products, and they are mainly extracted from plants. However, the development and utilization of flavonoids have been restricted due to its low bioavailability, difficulty in purification, the low content in plants, the massive waste and pollution caused by the traditional extraction process. This paper reviews the biosynthetic pathway, genetic engineering synthesis, microbial transformation, chemical synthesis and chemical structure modification of flavonoids, etc., which aims to provide references for further research, and the development and utilization of flavonoids.
  • JIANG Man
    Food and Fermentation Industries. 2021, 47(3): 259-264. https://doi.org/10.13995/j.cnki.11-1802/ts.025230
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    Due to the excellent environmental-friendly, stability against coalescence and potential applications in the delivery of bioactive drugs of Pickering emulsions, great progress has been made in cosmetics, industry, food and other fields. Compared with traditional emulsions, Pickering emulsion does not contain any surfactants, and it is safe and stable. The application of inorganic particles and synthetic particles are also been greatly restricted when considering its toxicity and food safety issues. In terms of stable emulsion, the adsorption of Pickering particles at the interface is almost irreversible. Therefore, it is necessary to find more suitable food-grade particles as Pickering emulsion stabilizers. This article reviews the stability mechanism of Pickering emulsions, including particle wettability, three-phase contact angle, steric hindrance of particle formation. And this review also summarizes the latest developments in the production, characterization and stability of protein and protein-based Pickering particles stabilized Pickering emulsions in recent years, and point out its development direction. This article will lay a foundation for further expanding the application of protein nanoparticles in the food field.
  • MA Yaqin, JIA Meng, ZHANG Chen
    Food and Fermentation Industries. 2021, 47(3): 265-273. https://doi.org/10.13995/j.cnki.11-1802/ts.025247
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    How to improve the sensory and nutritional quality of fruit juice products and guarantee of their safety in related processing fields have been the key challenges and research hot-spots in recent years, whereas non-thermal processing technology might be the potential thermal alternative due to its advantage preserving the original flavor and nutrition of fruit juice to the utmost extent under sterilizing. Among them, the high-pressure homogenization technology with developing from a traditional homogenization process to a non-thermal processing technology has been attracted attention. In this paper, the development history of high-pressure homogenization technology and equipment principles were introduced, the effects of high pressure homogenization treatment on key indicators such as juice cloud stability, nutritional quality, the number of microorganisms and enzyme activity were reviewed. And the development trends in the future was all been discussed in order to provide the theoretical support for the application of high pressure homogenization technology to the industrialization of fruit juice.
  • ZHANG Lan, XU Yongjian
    Food and Fermentation Industries. 2021, 47(3): 274-280. https://doi.org/10.13995/j.cnki.11-1802/ts.024994
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    As a natural antibacterial and antioxidant agent, plant essential oils had been studied and applied in the preservation of fruits and vegetables. Due to the long-term requirement for fresh-keeping, the microcapsule technology, which could effectively control the slow release of plant essential oils, has been aroused interests of researches. This paper mainly reviewed the structure, preparation and wall materials of plant essential oil microcapsules, as well as plant essential oil microcapsules application in fresh-keeping packaging of fruits and vegetables. This review aimed at providing research directions for plant essential oil microcapsules and had great significances for expanding the application of plant essential oils.
  • LIU Huifan, LIN Nuoyi, CHENG Jian, MA Lukai, CHEN Naiyi, FENG Haokai, WANG Qin
    Food and Fermentation Industries. 2021, 47(3): 281-287. https://doi.org/10.13995/j.cnki.11-1802/ts.026004
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    Active polysaccharides are safe, non-toxic, with physiological functions such as immune regulation, anti-oxidation, antibacterial, and blood sugar reduction. They are widely used in medicine and food industry, as well as in development of hygiene and health products. Microbial fermentation can make full use of the raw materials in preparing active polysaccharides. During fermentation, high-molecular substances that cannot be directly absorbed by the human body can be decomposed into small molecules, new active substances may be produced, and active ingredients might be more effective. Its advantages of reducing toxic and side effects have attracted much attention. In this review, methods for preparation of active polysaccharide by microbial fermentation were systematically summarized from the following aspects: fermentation strains, fermentation processes, polysaccharide synthesis pathways, and the structure and activity of fermented polysaccharides. Furthermore, the development prospects of active polysaccharides was proposed, to provide a reference for the in-depth development and application.
  • GE Yuzi, SUN Jingxian, HUANG Gang, JIN Gang
    Food and Fermentation Industries. 2021, 47(3): 288-293. https://doi.org/10.13995/j.cnki.11-1802/ts.026005
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    Malolactic fermentation (MLF) caused by lactic acid bacteria (LAB) is a necessary process in high-quality wine production. In this process, ethanol is the main factor that inhibit the activity of LAB. With the increase of sugar content in grapes caused by global warming, focus has been placed on the inhibitory effect of ethanol on LAB. Ethanol stress changes the cell structure of LAB and affects the MLF. Therefore, the growth and metabolism of LAB under ethanol stress are very important for wine production. The response mechanism of common LAB under ethanol stress during MLF is described, which provides a reference for the ethanol stress tolerance mechanism of LAB, as well as the screening of strains.
  • LI Jingzhu, HU Mengjun, ZHANG Jianhua
    Food and Fermentation Industries. 2021, 47(3): 294-301. https://doi.org/10.13995/j.cnki.11-1802/ts.026006
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    Protein-glutaminase (PG) has an efficient deamidation effect on plant proteins, which can increase the solubility of the protein and improve its functional properties, such as foaming and gelation.Therefore, it has huge application prospects in the industrial production of plant foods.However, due to the low fermentation yield of the original strain of PG-producing Chryseobacterium proteolyticum, heterologous recombinant expression is required to increase its yield.In this study, the PG gene was recombined into the plasmid pHT43, and the inducible promoter Pgrac of pHT43 was replaced by a strong constitutive promoter P43.Then, the recombinant plasmid was transformed into E.coli BL21 (DE3) strain to obtain an expression strain-pHT43:pro-mPG (P43)/BL21 (DE3).After cultured for 16 hours, the cells were collected and ultrasonically disrupted to release PG with a leader peptide.After centrifugation, the supernatant was purified by a nickel column, after desalination and concentration, the suspension was digested with 0.3 mg/mL trypsin to be catalyzed into a mature PG.The results of SDS-PAGE showed that the molecular weights of PG before and after trypsin hydrolysis were 38 kDa and 20 kDa, respectively.Western-blot results showed that both of them could bind to his-tag antibody.The enzyme activity of mature PG was 0.250 U/mL, and the optimal conditions for enzyme activity determination were 45 ℃ and pH 6.5.The final optimized fermentation conditions were as follows:4 g/L glutamine, 4 g/L sucrose, 1 g/L yeast extract, 8 g/L yeast extract, 2 g/L tryptone, 10 g/L sodium chloride, 3 g/L calcium chloride.Under above fermentation conditions for 16 h at 37 ℃ and pH 6.5, a final enzyme activity of 0.761 U/mL was achieved.
  • LIU Zhendong, LIU Hongli, JIANG Jingyu, LUO Zhang, LI Liang, XUE Bei
    Food and Fermentation Industries. 2021, 47(3): 302-308. https://doi.org/10.13995/j.cnki.11-1802/ts.026067
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    The effect of drying methods including natural drying, hot-air drying, vacuum drying and freeze drying on flavor of Tricholoma matsutake fruiting bodies were analyzed by electronic nose combined with GC-MS method. The result showed that the response profiles of radar sensors of T. matsutake fruiting bodies processed by different methods were roughly the same, however, the response intensity was different. The radar response values of the samples were vacuum drying > natural drying > freeze drying > hot-air drying, wherein the response value of vacuum drying sample was the highest, indicating that vacuum drying had the best effect on preserving the flavor of T. matsutake. The principal component analysis and discriminant function analysis diagrams showed that the electronic nose technology could well cover the flavor information of the four samples without interference, and thus can be used to distinguish and identify different samples. The results of GC-MS showed that 137 compounds were found in T. matsutake fruiting bodies processed by different methods, among which 109 were found in naturally dried sample and one was peculiar; 112 in hot-air dried sample and six were peculiar; 108 in freeze-dried sample and one was peculiar; 109 were vacuum-dried and seven were peculiar.
  • LIU Huifan, LIN Yingyu, ZHANG Huanyou, LI Yanfu, XIAO Gengsheng, WANG Qin
    Food and Fermentation Industries. 2021, 47(3): 309-316. https://doi.org/10.13995/j.cnki.11-1802/ts.026298
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    In order to optimize and control the processing conditions of black garlic, a fermented garlic product, single garlic and multi-clove garlic were used as raw materials to ferment black garlic via an ultrasonic assisted microwave technique and three stage variable temperature fermentation method. The physical and chemical properties of black garlic were determined by low field nuclear magnetic resonance (LF-NMR). The results showed that T2 free water activity decreased and gradually transferred to the surface of garlic cloves with the increase of processing time. The correlation analysis of physical and chemical properties showed that the characteristics of black garlic were related to its chemical properties. It was found that the properties of single black garlic changed regularly. The three-stage fermentation process improved the taste and quality of single black garlic, but the effect on multi-clove black garlic was inconspicuous. Finally, a simple grading model of black garlic quality was established, and garlic brightness was used as the main factor to evaluate the black garlic products, which provided a theoretical basis for the subsequent optimization and control of black garlic processing technology.