HUANG Gen-shu,XU Mei-juan,YANG Tao-wei,ZHANG Xian,RAO Zhi-ming,XU Zheng-hong
Food and Fermentation Industries. 2017, 43(6): 1.
Compared with the conventional fixed bed ion exchange process,continuous ion exchange process is better in continuity,stability and efficiency.Corynebacterium crenatum,a excellent L-arginine producer with domestic autonomous intellectual property right,is able to produce L-arginine with a production of 74.5 g/L.In this study,D155 was proved to be the most competent resin to recover the L-arginine in fermentation by static adsorption and desorption tests.Adsorption equilibrium could be achieved within 30 min with the equilibrium capacity of 121.31 mg/g.The optimal feed flow rate obtained from the fixed bed tests was 6 mL/min,at which the breakthrough time,subsaturation time,saturation was 29,59,86 min,respectively.The corresponding breakthrough capacity,subsaturation capacity and saturation capacity was 134.7,193.8,261.2 mg/g,respectively.The optimal eluent was 2 mol/L NH3 ·H2O,which could result in elution rate of 97.14％.Based on the optimal conditions obtained in fixed bed experiments,continuous ion exchange system consisted of 30 columns was designed,and the optimal flow rates of adsorption zone,rinsing zone after adsorption,desorption zone,rinsing zone after desorption,excluding water zone were 6,6,5,10,3 mL/min,respectively.The designed continuous ion exchange system could operate steady at the optimal flow rates,and the outlet concentration of each zone changed periodically.A more scientific recovery process of L-arginine in industrial fermentation was obtained herein,which not only improved the process continuity,stability and efficiency,but also reduced the consumption of water,base and labour.
