15 November 2019, Volume 45 Issue 21
    

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  • CONG Shanzi, TIAN Kangming, ZHANG Xin, LU Fuping
    Food and Fermentation Industries. 2019, 45(21): 1-7. https://doi.org/10.13995/j.cnki.11-1802/ts.020595
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    The novel lipase gene (tglE) from Aspergillus niger was cloned and expressed in Pichia pastoris. The recombinant TglE had typical lipase activity with the maximal activity at 40 ℃ and pH 7.0. The enzyme retained over 60% activities at 30 ℃-60 ℃ or pH 6.5-9.0 and was stable at 20-30 ℃ or pH 6.0-8.0. The catalytic activity of TglE was significantly improved by Cu2+, Mn2+, Ca2+, K+, Mg2+, and Sn2+ but inhibited by Fe3+, Zn2+ and EDTA. The prefered substrates for tglE were chemically synthesized pNPB and natural olive oil. The Km and Vmax for pNPB were 14.40 mmol/L and 46.72 mmol/(mL·h), respectively. In addition to the hydrolysis activity, tglE exhibited significant esterification efficiency for ethyl caprylate synthesis from octanoic acid and ethanol.
  • DENG Xiaoying, YANG Yu, JIANG Yang, LIU Xiaoming, ZHAO Jianxin, ZHANG Hao, CHEN Wei
    Food and Fermentation Industries. 2019, 45(21): 8-14. https://doi.org/10.13995/j.cnki.11-1802/ts.020100
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    The effects of phosphate-β-galactosidase and β-galactosidase on lactose metabolism in Lactococcus lactis (L. lactis) were investigated. The growth, acid production, lactose metabolism, activities of β-galactosidase and phosphate-β-galactosidase of 12 strains were determined in MRS-lactose broth where lactose was the sole carbon source. In order to understand the differences between these two galactosidases, the galactose accumulation in the fermentation broth was determined. The results showed that the lactose metabolism of L. lactis had significant differences. Moreover, the galactose contents in the fermentation broth of strains with high β-galactosidase activities ranged from 270 mg/L to 4110 mg/L, while the galactose contents of strains with high phosphate-β-galactosidase activities were in the range of 40 mg/L to 900 mg/L. Combining L. lactis, which had high phosphate-β-galactosidase activities, with Streptococcus thermophilus could reduce the content of galactose. The experimental results showed that L. lactis with high phosphate-β-galactosidase activities contributed to the reduction of the galactose content in fermented dairy products.
  • WANG Jingchun, TIAN Kangming, MIAO Jia, WANG Caizhe, JIN Peng, WANG Zhengxiang
    Food and Fermentation Industries. 2019, 45(21): 15-21. https://doi.org/10.13995/j.cnki.11-1802/ts.020729
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    In order to improve the tolerance of ethanologenic Escherichia coli to substrate and ethanol and the performance of ethanol fermentation, the trehalose metabolic pathway of E. coli B0013-1031H was engineered and the mutants JC31 and JC41 were constructed. In strain JC31, the trehalose catabolism pathways were deleted, the trehalose synthesis pathway was further enhanced and resulted in strain JC41. The ability of trehalose synthesis and accumulation of JC31 and JC41 was higher than that of the original strain, in which, JC41 intracellularly accumulated trehalose up to 12-fold that of the original strain. The tolerance of JC31 and JC41 to glucose and ethanol stress was significantly improved. The ethanologenic recombinant E. coli JC31-PA obtained by engineering ethanol synthesis pathway fermented 120 g/L of glucose and yielded 50.6 g/L of ethanol, which was 5.42% higher than that of the control strain. The conversion rate of ethanol from glucose was 48.72 g/100 g glucose, which was 12.67% higher than that of the control.
  • YANG Shengyuan, LIN Qian, LAI Liping, HUANG Tingting
    Food and Fermentation Industries. 2019, 45(21): 22-30. https://doi.org/10.13995/j.cnki.11-1802/ts.020312
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    In order to develop high-quality glutamate decarboxylase (GAD, EC4.1.1.15) for efficient immobilization, the construction of cellulose-binding domain (CBD) GAD fusion enzyme (CBD-GAD) and its enzymatic properties were investigated using Enterococcus faecium GDMCC60203 as gadB donor. The results showed that the recombinant Escherichia coli GDMCC60445 carrying pRPOCB-EfagadB could efficiently express CBD-GAD, and the activity of the cellulose immobilized CBD-GAD prepared from 120 mL fermentation broth was 347.93±27.63 U/g. When continuously transferred five batches in LB medium without ampicillin, the capability of CBD-GAD expression in the recombinant E. coli GDMCC60445 was still stable. The CBD-GAD purified by one-step purification showed a single band on SDS-PAGE electrophoresis with a molecular weight of 74.02 kDa. The purified CBD-GAD showed maximum activities at pH 4.6 and 60 ℃, respectively, and was stable in the range of pH 4.8 to 5.6 and temperature from 4 to 40 ℃. CBD-GAD reacted only with L-glutamic acid with apparent Km and Vmax of 10.58 mmol/L and 3.83 μmol/(mL·min), respectively, and the reaction activities were not inhibited by substrate and product. Hence, this strain has the potential to be applied in industrial production of gamma-aminobutyric acid and D-glutamic acid due to its high stability and excellent enzymatic properties.
  • SUN Sijia, ZHAI Lei, BAI Xiubin, XU Ling, YU Panpan, YAO Su
    Food and Fermentation Industries. 2019, 45(21): 31-37. https://doi.org/10.13995/j.cnki.11-1802/ts.019982
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    This paper firstly studied the enzyme production conditions of a high amylase-producing Saccharomycopsis fibuligera screened from high-temperature Daqu of Sesame-flavor Baijiu. Using single factor test and response surface analysis, the optimal conditions were obtained: 20 g of raw material, 51% water content, 18% inoculum and fermented at 24 ℃. Under the optimal fermentation conditions, the amylase activity reached 12 297 U/g after 72 h of solid-state fermentation, which was 63.96% higher than that before optimization. Besides, the enzymatic properties of amylase from this strain were characterized. The optimal reaction temperature was 50 ℃ and the optimal pH was 4.0. The experimental results showed that the strain was effective in producing amylase and the amylase was adaptable to the acidic and high-temperature environment for Daqu fermentation. This strain has the potential in high-temperature Daqu production.
  • ZHANG Wenping, ZHAO Yingjie, LUO Sheng, CHENG Xin
    Food and Fermentation Industries. 2019, 45(21): 38-45. https://doi.org/10.13995/j.cnki.11-1802/ts.021589
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    In order to obtain the lactic acid bacteria (LAB) with high exopolysaccharide (EPS)-producing ability, a strain of LPC-1 was finally screened by observing whether they could form sticky colonies and sulfuric acid-phenol method, which was identified as Lactobacillus plantarum. Using the yield of extracellular polysaccharide as an indicator, the fermentation process was optimized by single factor and response surface tests. The results showed that the optimal culture conditions were: 30 g/L sucrose and 10 g/L soybean peptone, inoculation amount of 4%, fermented at 30 ℃ and pH 6.5 for 24 h. The production of EPS was significantly affected by temperature, pH and concentration of diammonium citrate as determined by the Plackett-Burman test. Combined with the central combination test and response surface analysis, the optimal fermentation conditions were 32 ℃, pH 6.7 and 3 g/L diammonium citrate. Under this condition, the EPS yield was 2 064.69 mg/L, which was consistent with the predicted value and increased by 48.64% compared with that before optimization. These results provided a basis for the scale production of EPS by LAB.
  • ZHAO Jiawei, AO Xiaolin, CAI Yimin, LIU Shuliang, CHEN Anjun,WAN Hu, XU Fei, WANG Fan, HE Jinyang
    Food and Fermentation Industries. 2019, 45(21): 46-52. https://doi.org/10.13995/j.cnki.11-1802/ts.021544
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    The most effective metal ion was selected and the optimal culture condition was determined for RS66CD biofilm formation. The tolerance of biofilm to temperature, pH, salt concentration and bile salt was also studied. After adding Na+, Mn2+, Ca2+, Mg2+ and Fe3+, biofilm formation was determined by the 96-well method and SEM and then the best metal ion was selected. Under different ion concentrations, growth temperatures, and growth times, the biofilm formation was also studied to identify the optimal condition. The result showed that NaCl concentration of 53 g/L, the culture time of 24 h, and the culture temperature of 40 ℃ were the optimal condition for RS66CD biofilm formation. The environmental tolerance of strains after biofilm formation was improved. Na+ can promote the biofilm formation of Lactobacillus plantarum. In industrial production, microorganisms can be kept active by biofilms under stress condition.
  • CAO Weihua, LUO Na, SUN Yixuan, TU Jingxia, LIU Jing, WANG Deliang, HAO Jianqin, LUAN Chunguang, BAO Yihong
    Food and Fermentation Industries. 2019, 45(21): 53-59. https://doi.org/10.13995/j.cnki.11-1802/ts.022197
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    A species-specific PCR detection system was established for beer spoilage bacteria Lactobacillus acetotolerans CN247. Three sets of species-specific primers were designed and optimized in SYBR GREEN real-time PCR detection system. A standard operating procedure (SOP) for qualitative and quantitative detection of Lactobacillus acetotolerans in beer samples was obtained. The results showed that the detection limit for Lactobacillus acetotolerans reached 102 CFU/mL and the detection accuracy reached 101 CFU/mL by using the detection system developed here. The results could be obtained within 48 h after pre-enrichment treatment by using NBB media. This research developed a Lactobacillus acetotolerans species-specific detection system which could meet the quality control requirements of the manufacturer in beer industry.
  • CUI Xiangxiang, BAI Feirong, YU Xuejian, BAI Xiubin, XU Ling, YU Panpan, YAO Su
    Food and Fermentation Industries. 2019, 45(21): 60-67. https://doi.org/10.13995/j.cnki.11-1802/ts.021012
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    Aroma-producing Eurotium chevalieri CICC 41584 isolated from Baijiu Daqu was applied in the production of aroma Baijiu Daqu in order to improve the flavor characteristics of Daqu. The volatile flavor compounds of liquid shaking flask fermentation medium and solid-state fermentation Daqu were analyzed by headspace solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) technology, combined with sensory threshold and relative odor activity value (ROAV). The results showed that the key flavor compounds (ROAV≥1)of the liquid fermentation broth of Eurotium chevalieri CICC 41584 were 1-octene-3-ol, linalool (linalol), decanal and citronellol, of which the ROAV of 1-octene-3-ol and linalool were 100 and 26.87, respectively, which contributed greatly to the overall flavor of the fermentation broth, presenting green grass or green tea flavor. 1-octen-3-ol, linalool, 3-methylbutyraldehyde, 4-ethylguaiacol, acetic acid, isovaleric acid were the key flavor substances in solid-state fermentation of daqu by CICC 41584, and the ROAV values were 100, 19.83, 19.38, 11.39, 6.88, 3.93, respectively, presenting the tea-flavor flavor dominated by 1-octene-3-ol and linalool. Eurotium chevalieri CICC 41584 can improve the flavor of Baijiu Daqu and has further application prospects.
  • HU Jinghui, CHANG Qiang, JIANG Chao, SONG Tao, CHEN Bin, JIANG Wei, HAN Xinglin, LI Nan
    Food and Fermentation Industries. 2019, 45(21): 68-72. https://doi.org/10.13995/j.cnki.11-1802/ts.021488
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    13 samples of Strong-flavor Baijiu raw liquor were analyzed by sensory evaluation method, and the samples were divided into 3 categories according to the level of different soft-sweet. By comparing the flavor compounds composition of the samples, it can be concluded that the flavor compounds of lactic acid and ethyl lactate are relatively high in the category A liquor samples with the most soft-sweet flavor. The ethyl lactate to ethyl hexanoate ratio was stably between 1.2 and 1.5, the ethyl acetate to ethyl lactate ratio was stably below 1.0, the acid to ethyl ratio was stable around 0.6, and the alcohol to ethyl ratio was controlled between 0.15 and 0.20. Multiple linear regression equation was used to establish the index model of soft-sweet’s sense in Strong-flavor Baijiu, in which the ethyl lactate to ethyl hexanoate ratio and the acid to ethyl ratio had a positive effect on the sense of soft-sweet in liquor, and the higher ratio had a positive effect on the improvement of soft- sweet’s level in liquor within limits.
  • LI Ke, PAN Lihong, LUO Xiaohu, WANG Li, WANG Ren , DU Zhihong, LI Caiming, CHEN Zhengxing
    Food and Fermentation Industries. 2019, 45(21): 73-78. https://doi.org/10.13995/j.cnki.11-1802/ts.019694
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    Electron Beam Irradiation (EBI) was used to degrade zearalenone (ZEN) and deoxynivalenol (DON). The degradation effects of EBI on single and mixed toxins were investigated, and the regularities of degradation and interaction of toxins were analyzed. The results showed that the degradation rate of single toxin in standard solution increased with the increase of EBI dose in the range of 0-10 kGy, and low initial concentration of toxin contributed to high degradation rate. At 10 kGy, the degradation rates of ZEN and DON at 1 ìg/mL were 76.04% and 89.31%, respectively. Using single toxin group as control, with EBI treatment on DON and ZEN mixture, the degradation rates of ZEN in standard solution and corn syrup were significantly reduced, while DON degradation rates showed no obvious change. In addition, the degradation rates of ZEN and DON in the corn flour with 14.20% (w/w) moisture content were both lower than that of the control group. When the water content was 19.80% (w/w), the degradation rate of ZEN was lower than that of the control group and DON was higher than the control group. This study clarifies the effects of EBI on the simultaneous degradation of ZEN and DON in corn, which can provide a theoretical reference for the application of EBI in the degradation of food mycotoxins.
  • QIN Peng, LU Dengxue, KANG Hongmei, HAN Rongbing, GUO Rui,ZHAO Yuhui, WEI Jiaqian, WANG Zhiye
    Food and Fermentation Industries. 2019, 45(21): 79-85. https://doi.org/10.13995/j.cnki.11-1802/ts.021831
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    Taking Cordyceps militaris as an illustrative example, a novel method that opened up an optional way for the isolation of fungal single-spores based on semisolid methylcellulose medium was developed. Optimized methylcellulose medium and traditional methods (spread plate, micromanipulation, limited dilution, and agarose medium) were used to isolate single-spores of Cordyceps militaris spore suspension that theoretically contained 150 living spores. Comparing the number of partible single colonies, operation simplicity and the efficiency of single-spore isolation, the feasibility of this novel method to isolate fungal single-spores was investigated. The optimized concentration of methylcellulose in semisolid medium was 23 g/L. The number of partible single colonies obtained using the optimized methylcellulose medium, spread plate, micromanipulation, limited dilution and optimized agarose medium reached 96、70、37、35 and 26, respectively. This novel method was proved to be the simplest, most convenient and efficient among these methods. Fungal single-spores could be simply, efficiently and conveniently isolated using optimized methylcellulose medium. The applied potential of the novel method is superior to that of traditional methods.
  • TANG Xiaoman, TANG Yao, ZHANG Qisheng, WANG Dongdong, CHEN Gong, LI Heng, MING Jianying, YU Wenhua, LIU Qingbin
    Food and Fermentation Industries. 2019, 45(21): 86-92. https://doi.org/10.13995/j.cnki.11-1802/ts.021627
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    This study aimed to explore the relationship between the metabolic pathway of major biogenic amines in Sichuan industrial pickled cowpea and microorganisms and enzymes. The content of biogenic amines and amino acids in Sichuan industrial pickled cowpea was determined, and the metagenomics were sequenced on the Illumina HiSeq 4000 platform and annotated on the NR and KEGG databases. The results showed that the main biogenic amines in the sample were cadaverine, putrescine, histamine and tyramine. The relative abundance (>0.5%) of microbial species was mainly related to the formation and degradation of putrescine, cadaverine and tyramine. The species of bioamine formation and degradation capacity were as high as 51.05%, mainly lactic acid bacteria, yeast and spores. Microorganisms such as Sugiyamaella lignohabitans and Geotrichum candidum contained enzymes both for the formation and degradation of putrescine, and can participate in different metabolic pathways for the formation and degradation of different biogenic amines. This research comprehensively expounds the relationship between the formation and degradation of four major biogenic amines and the microbes and enzymes in the pickled cowpea system, which can lay foundation for the practical application of kimchi industry to provide data supporting and control of biogenic amines.
  • WANG Meng, LAN Weiqing, QIU Zehui, FU Zixin, GONG Taoshuo, XIE Jing
    Food and Fermentation Industries. 2019, 45(21): 93-101. https://doi.org/10.13995/j.cnki.11-1802/ts.021339
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    In order to investigate the effects of apple polyphenols (AP) treatment on the comprehensive quality changes of large yellow croaker (Pseudosciaena crocea) during ice storage, samples were treated with 0.25 g/L (AP1), 0.50 g/L (AP2) and 1.00 g/L (AP3) apple polyphenols respectively, and that of sterile water treatment was used as control group (CK). pH value, color difference, thiobarbituric acid (TBA), total volatile base nitrogen (TVB-N), total sulfhydryl content, total viable count (TVC), psychrophilic bacteria count (PBC) and sensory evaluation during storage were analyzed, and the change of water migration was evaluated by water holding capacity (WHC), low-field nuclear magnetic resonance (LF-NMR) and magnetic resonance imaging (MRI). The results showed that TBA value of CK group was increased to 1.94±0.236 mg MDA/kg at day 12, while that of AP3 group did not exceed 1.5 mg MDA/kg. After treated with apple polyphenol, the pH, TVB-N, TVC and PBC values of Pseudosciaena crocea were inhibited, the decrease of total sulfhydryl content slowed, the migration rate of free water in fish reduced, and the color change of samples was delayed, with benefitial effects on improving water retention and sensory properties. Therefore, 1.00 g/L apple polyphenols could prolong the shelf life of large yellow croaker (Pseudosciaena crocea) during ice storage for another 2-4 d. The research can provide a theoretical reference for the application of apple polyphenols in the preservation of aquatic products.
  • WU Yanyan, TAO Wenbin, HAO Zhiming, LIN Wanling, YANG Xianqing, YANG Shaoling, WEI Ya, PAN Chuang
    Food and Fermentation Industries. 2019, 45(21): 102-109. https://doi.org/10.13995/j.cnki.11-1802/ts.021273
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    In order to clarify the effect of salt on the quality of fish meat, the effects of different salinity (6%, 9%, 12%, 15%, g/g) on the protein, texture, pH and volatile flavor of large yellow croaker during pickling were studied. The results showed that the salt-soluble protein content and total sulfhydryl content were stable when salt content of fish was over 9%, while the effects on protein, pH and texture quality were significant (P<0.05). The total nitrogen content increased first and then decreased, while the non-protein nitrogen and protein hydrolyzed index were opposite. The pH value showed a downward trend, and the hardness as well as elasticity of fish meat showed an upward trend. The chewiness decreased first and increased afterwards. Moreover, the volatile flavor component of fish meat was mainly hydrocarbons with the salt content less than 6%. When salinity was over 9%, volatile flavors contributed the most, up to 65 species, mainly including alcohols, aldehydes, and ketones, etc. Therefore, the quality and flavor of large yellow croaker can be better maintained at salt concentration of 9%, which provides a technical support for the salting process of the large yellow croaker.
  • LU Xiaochuan, ZHANG Yinong, PAN Chenglei, KANG Mengyao, LI Yixuan, SHANG Yongbiao
    Food and Fermentation Industries. 2019, 45(21): 110-117. https://doi.org/10.13995/j.cnki.11-1802/ts.021237
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    The purpose of this study was to investigate the antioxidant effects of cumin essential oil on myofibrillar protein (MP) of mutton during super-chilling storage (-3 ℃). Taking 2 mg/mL tea polyphenol (TP) and 0.15 mg/mL dibutylhydroxytoluene (BHT) treated mutton as positive control, the mutton was soaked with 8 mg/mL, 12 mg/mL and 16 mg/mL cumin essential oil, and the changes in MP structure and function indicators were examined during super-chilling storage. With the prolongation of storage time, the carbonyl content and surface hydrophobicity of MP in all groups increased, the content of sulfhydryl, solubility and emulsification decreased, and the elastic modulus (G′) showed a rise-decline-rise trend. Raman spectroscopy indicated that the relative content of -helical structure decreased, while the relative content of -sheet and random coil increased. The cumin essential oil could significantly delay above mentioned changes, and its effect was better than TP and BHT with the concentration above 12 mg/mL. The cumin essential oil can inhibit the oxidation of mutton MP during super-chilling storage, reduce the damage of secondary structure as well as maintain the functional properties of MP, which shows a good prospect to apply the cumin essential oil in the field of protein antioxidation.
  • LI Song, GUO Quanyou, LI Baoguo, JIANG Chaojun, YANG Xu, ZHANG Xiujie, ZHANG Yongxing
    Food and Fermentation Industries. 2019, 45(21): 118-125. https://doi.org/10.13995/j.cnki.11-1802/ts.021448
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    This paper was conducted to evaluate the effects of aquaculture model and diet on the quality of cultured Pseudosciaena crocea. With wild P.crocea as reference, the differences of body color, texture and nutritional components of cultured P.crocea (group R1 fed with fresh bait in raft cage, group R2 fed with fish feed in raft cage, group S1 fed with fresh bait in multi-connected cage, group S2 fed with fish feed in multi-connected cage) were analyzed. The results showed that with a certain regularity, the inner and outer color of the rear tail were similar, but significantly different from the whole. The body color of the abdomen and the back showed a shallow distribution on the edge and dark in the middle. The addition of Antarctic krill in the feed could significantly increase the yellowness value in abdomen. Moreover, group S possessed better texture, higher water holding capacity, polyunsaturated fatty acids content, essential amino acids content, protein content and lower crude fat content, while the feed group (R2, S2) had higher content of b*, fatty acids and amino acids. In conclusion, the body color, fish quality and nutritional components of P.crocea fed in multi-connected cage are better than those of wild fish, which can provide a basis for optimizing the breeding model and developing a high-efficiency and environmental-friendly feed formula to improve the quality of P.crocea.
  • XIA Luyang, LIU Zhaolong, XIONG Guoyuan, MENG Congjun, WEI Youbing, TAO Hongbin
    Food and Fermentation Industries. 2019, 45(21): 126-133. https://doi.org/10.13995/j.cnki.11-1802/ts.021135
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    In order to fully understand the processing characteristics of local pork in Anhui province, sensory evaluation, yield, texture, TBA and rheological properties of Weizhu pork (WP) and Chengling Black pork (CBP) were analyzed during cooking, frying, roasting and chopping. The results showed that the sensory evaluation scores of WP and CBP were superior to commercial pork (CP) among all four processing methods. In addition, the yield of boiled pork was the highest, followed by chopping & seasoning, while the hardness and cohesiveness were the lowest in cooking. In frying, the hardness and chewiness of WP and CBP were the most, while the value of TBA was significantly lower than that of CP (P<0.05), with CBP the lowest. The hardness, cohesiveness and chewiness of CP were the highest during four processing methods. After chopping, viscoelastic colloids were obtained, and the storage modulus of WP and CBP were significantly higher than that of CP (P<0.05), yet the loss modulus of CBP was significantly higher than that of the others. In conclusion, the quality of local pork (WP and CBP) was better than CP during processing. WP may be more suitable for boiling and chopping and CBP for frying. The results provide a reference and theoretical guidance for the indepth processing and utilization of WP and CBP.
  • WANG Dongkun, CHEN Ding, GUO Na, ZHANG Fangyan, ZHU Guilan
    Food and Fermentation Industries. 2019, 45(21): 134-138. https://doi.org/10.13995/j.cnki.11-1802/ts.021198
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    A new edible coating method was developed to solve the problem of water chestnuts spoilage and water loss during storage. The edible composite film was prepared by gellan gum, guar gum and different concentrations of Nisin, followed by antibacterial activity analysis using inhibition zone test. The fresh Chinese water chestnut was coated with edible film, and the changes of related indexes were determined during storage. The composite film exhibited effective inhibition against Bacillus subtilis, but showed a low inhibitory effect on Escherichia coli and Saccharomyces cerevisiae. Besides, the antimicrobial activity of the composite films was enhanced with the increase of Nisin concentration. At the same time, the results of coating experiment on water chestnuts showed that gellan-guar gum composite film could inhibit the deterioration during storage and 0.5 g/L Nisin-gellan-guar gum composite film had significant fresh-keeping effect. For example, after 25 d of storage, the good fruit rate was 51% and POD activity reached 0.83 U/g, which was significantly higher than that of control group (30%, 0.68 U/g). Though the relative conductivity of pulp was 3.5% lower than that of the control group, it could maintain good texture of water chestnuts. Nisin-gellan-guar gum composite film can be used for the preservation of water chestnuts.
  • YU Jie, ZHANG Yuchen, XIE Jing
    Food and Fermentation Industries. 2019, 45(21): 139-146. https://doi.org/10.13995/j.cnki.11-1802/ts.021379
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    This paper was conducted to investigate the irradiation effects of UV-A light on the fresh-cut spinach. Based on primary screening experiment, 420s (1.764 kJ/m2) was chosen as the best daily irradiation dose with the shelf life extended to 10 d. The fresh-cut spinach was irradiated for 24h (group A), 8h (group B) and 4h (group C) by UV-A. Physicochemical indexes including total viable counts, changes of water content and activities of antioxidant enzymes were determined to explore the effects of different irradiation frequencies on fresh-cut spinach. The results showed that the preservation effect was arranged in descending order of group A>group B>group C in the first 6 days. After 6 days, the content of each nutrient index and activities of antioxidant enzymes were the highest in group B on the 12th day, which indicated that group B was more conducive to long-term storage of spinach, followed by group C. UV-A light can significantly improve the antioxidant capacity of fresh-cut spinach, and different irradiation methods can be selected according to different fresh-keeping purposes, which provides a new improvement on the fresh-cut spinach preservation for supermarkets in the process of logistics and transportation.
  • WANG Jing, HONG Min, FENG Yu, HE Mingyang, WANG Rikui, YU Zuixin, ZHOU Lian
    Food and Fermentation Industries. 2019, 45(21): 147-154. https://doi.org/10.13995/j.cnki.11-1802/ts.021282
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    To explore the effects of exogenous melatonin treatment on postharvest flavor and fruit quality in citrus fruit, ‘Aiyuan 38’ citrus after harvest were immersed with 1.0 g/L melatonin to determine the changes of fruit quality and relevant indicators of ethanol metabolism during storage at low temperature (6~8 ℃). The results showed that compared with the control group, the freshness and the Vitamin C (Vc) content of citrus were significantly increased by 21.40% and 8.85% (P<0.05), and melatonin maintained the soluble contents(TSS), and inhibited the respiratory intensity. On the 60th day of storage, the pyruvate content of citrus treated with melatonin increased by 17.42%, while the ethanol and acetaldehyde content decreased by 52.21% and 36.85% (P<0.05), respectively. In the meantime, the gene expression and enzymatic activities of pyruvate decarboxylase (PDC) and alcohol dehydrogenase (ADH) showed a downtrend. Treatment with melatonin at the concentration of 1.0 g/L can effectively reduce the accumulation of ethanol in citrus fruits and improve the fruit quality, which provides a theoretical reference for postharvest regulation of fruit flavor quality.
  • HU Yuanran, CHEN Dixin, HU Lihong, DONG Haiqing, YANG Yingjun
    Food and Fermentation Industries. 2019, 45(21): 155-160. https://doi.org/10.13995/j.cnki.11-1802/ts.020987
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    In order to evaluate the fresh-keeping effect of disodium ethylenediamine tetraacetate (EDTA-2Na) solution on ‘Huangguan’, ‘Hongxiangsu’ and ‘Dangshan’ pears, three kinds of fresh-cut pears were immersed in different concentrations of EDTA-2Na solution for 3 min and stored at 4 ℃. Changes of physiological and biochemical indexes of fresh-cut pears during storage for 8 d were determined. The results showed that different concentrations of EDTA-2Na solution had different preservation effects on three varieties of pears. 30 g/L EDTA-2Na solution treatment could significantly delay the decrease of hardness and lost of soluble solid content, and inhibit the increase of relative conductivity and polyphenol oxidase (PPO) activity. In terms of inhibiting microbial growth, the total number of colonies of the three pears treated with this concentration decreased by 46.2%, 56.9% and 56.8%, respectively, on the 8th day compared with the control. The content of vitamin C (VC) in ‘Huangguan’ and ‘Dangshan’ pears was 61.6% and 70.9% higher than that in the control on the 8th day. In short, EDTA-2Na solution has a positive effect on the storage quality of fresh-cut pears, and in particular, 30 g/L EDTA-2Na solution has the optimum comprehensive effect.
  • ZENG Xiaofeng, SHANG Sang, CHEN Shuang, ZENG Shunde, GAO Lunjiang, YIN Xumin, DIAO Yuan
    Food and Fermentation Industries. 2019, 45(21): 161-166. https://doi.org/10.13995/j.cnki.11-1802/ts.020549
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    To explore the suitable sterilization method for blood orange juice, heat treatment (80 ℃,15 min) and microwave sterilization (800 W,90 s) were used to analyze the effects on quality and antioxidant activity of blood orange juice during storage. The results indicated that there was no significant difference in pH, titratable acid, soluble solid and reducing sugar between the two sterilization methods. The antioxidant vitamin C, anthocyanin, total phenols and total flavonoids showed a decreasing trend with the increase of storage time, but microwave sterilization could better retain the nutrients. The antioxidant capacity of DPPH+ and ABTS+ also decreased, and the content of anthocyanin and vitamin C were significantly correlated with the antioxidant capacity of DPPH+ and ABTS+, with the correlation coefficient between 0.910 and 0.965. The DPS analysis showed that the antioxidant effect of blood orange juice was mainly from the direct positive effect of anthocyanin, which contributed the most to the antioxidant capacity of blood orange juice. Therefore, microwave sterilization supply better quality assurance of blood orange juice, which provides a technical reference for the sterilization of blood orange juice in the future.
  • ZHANG Ying, WANG Yuxiang, ZHANG Jinjin, SUN Jing, CAO Wei
    Food and Fermentation Industries. 2019, 45(21): 167-172. https://doi.org/10.13995/j.cnki.11-1802/ts.021446
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    The removal of hazardous substances from honey is beneficial for the utilization in food processing or other fields, which will contribute to realize the resource reuse effectively. By virtue of macroporous resin adsorption technology, the effects of resin type and process conditions on the removal of 5-hydroxymethylfurfural (5-HMF) from 5-HMF positive honey sample were investigated, and the physicochemical indexes and phenolic ingredients of honey before and after resin adsorption were analyzed, as well as the macroporous resin applicability to different types of honey. The results showed that weak polar resin was suitable for removing 5-HMF from honey, among which LSI-3 resin had the best removal effect. Reducing temperature, increasing resin content and prolonging adsorption time contributed to 5-HMF removal, and the removal rate reached 62.49% under the optimum adsorption condition of 25 ℃, 44 g/kg resin content and 80 min for LSI-3 resin. In addition, LSI-3 resin adsorption did not significantly affect the conductivity, pH and total reducing sugar content of honey, but the amylase value decreased slightly, and phenolic components decreased obviously, showing a good applicability for removing 5-HMF from different kinds of honey. This provides a new approach for treatment of honey with high 5-HMF content.
  • ZHOU Yan, ZHAO Cunchao, SHI Chongying, TAO Liang, TIAN Yang
    Food and Fermentation Industries. 2019, 45(21): 173-179. https://doi.org/10.13995/j.cnki.11-1802/ts.021188
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    To develop a walnut milk beverage enriched with organic calcium, peeled walnut and Moringa oleifera leaf were fermented to extract organic calcium, and on the basis of single factor experiment, the processing technology was optimized by response surface methodology. The optimal technological process was peeling, soaking, beating with solid-liquid ratio of 1∶1 (1min for 1 kg walnut colloid mill), milling with solid-liquid ratio of 1∶3 (grinding wheel), filtering (200 mesh), blending (Moringa oleifera calcium powder of 40 g/kg, sucrose of 42 g/kg, vanillin of 0.15 g/kg, pH 7.6-7.8), preheating (50-60 ℃), homogenizing (primary homogenization of 20-25 MPa, secondary homogenization of 35-40 MPa), vacuum degassing, filling and sterilization (121 ℃, 15 min). Besides, the optimum addition of glycerol monostearate and sucrose fatty acid ester mixture (mass ratio of 7∶3) was 0.1% (volume fraction), and the addition of pectin and sodium alginate mixture (mass ratio of 4∶6) was 0.15% (volume fraction). The high-calcium walnut milk had unique flavor and high stability, with organic calcium content over 120 g/100 mL. The study provides a technical support for the development of new walnut products.
  • ZHANG Qing, YUAN Yuan, DENG Yanglong, ZHOU Xiangyu, FAN Guiling, LI Yufeng
    Food and Fermentation Industries. 2019, 45(21): 180-186. https://doi.org/10.13995/j.cnki.11-1802/ts.021119
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    Using thin-shelled walnut as raw material, the effects of enzymatic hydrolysis on the concentration of walnut peptides were studied. By single factor experiment, taking walnut peptides concentration as the monitoring index, the suitable ranges of hydrolysis temperature, pH, time and enzyme dosage were determined. On this basis, the response surface analysis was carried out with the concentration of walnut peptides as the response value, and the technological conditions of alkaline protease hydrolysis were optimized by Box-Behnken central combination test. The results showed that the optimum conditions of enzymatic hydrolysis were enzymatic hydrolysis temperature of 50.24 ℃, enzyme dosage of 2.03%, pH 7.13, and hydrolysis time of 4.2h. Under these conditions, the concentration of peptides reached 2.55 mg/mL, more than two times higher than before. The error between the test results and the optimization results was less than 2%, indicating a reliable optimization result. The optimization of enzymatic hydrolysis process is of great significance to improve the utilization of walnut protein, and provides a theoretical basis for the industrial application of walnut peptides.
  • HU Xiaobo, LI Mengyuan, LIU Yong, WANG Junhui
    Food and Fermentation Industries. 2019, 45(21): 187-194. https://doi.org/10.13995/j.cnki.11-1802/ts.021341
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    The emulsification properties of pectins extracted from orange peel by different methods were investigated. Sequential extraction (thermal buffer solution, chelating agent solution, dilute alkali solution and concentrated alkali solution), acid extraction and enzyme extraction were used to obtain 6 different pectins from orange peel (HBOP, CHOP, DAOP, CAOP, AEOP and EEOP). The effects of pectin concentrations, pH, Ca2+ concentrations and temperature on emulsification properties of 6 pectins were studied. The emulsification activity and stability of CAOP was enhanced with the increase of pectin concentration, reaching the maximum at the concentration of 10 mg/mL, surpassed only by HBOP and CHOP. Besides, the emulsification activity of CAOP was always higher than 0.05 at any pH or Ca2+ concentration, and the maximal emulsion stability was 108 min at pH 5. The 6 different pectins showed good emulsifying activity and stability at room temperature. CAOP had lower flocculation index (FI) compared with other pectins (FI≤279.09), and had small droplet size, with the maximum of 33.44 ìm. In conclusion, CAOP exhibited ideal emulsification property, which can be used as a potential substitute for food emulsifier.
  • CHEN Ju, CHEN Lin, HU Tao, ZHANG Xiaofeng, SONG Jia, ZHENG Yu, SHI Lei, WAN Shoupeng, WANG Min
    Food and Fermentation Industries. 2019, 45(21): 195-200. https://doi.org/10.13995/j.cnki.11-1802/ts.021724
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    For identifying the critical sites to prevent microbial contamination, the microorganisms composition of apple vinegar beverage production and their characteristic were analyzed. High-throughput sequencing was used to reveal the bacterial composition of the samples. The main microorganisms were separated, and then the characteristics of potentially contaminating bacteria were analyzed. Six genera, including Pediococcus, Gluconacetobacter, Lactobacillus, Paracoccus, Lysobacter and Aquatobacterium were detected in the raw apple vinegar flocculent that can cause the turbidity of apple vinegar beverage and result in the increase of lactic acid concentration by 2.3-fold. According to the production process, 11 critical sites of apple vinegar beverage production were sampled and analyzed. Especially, the heatproof microorganisms were purified and identified. The results showed that the potential contaminating microorganisms for the apple vinegar beverage were mainly consisted of 15 species of bacteria belonging to 7 genera. The connecting pipes between each production section are the critical sites to prevent microbial contamination for apple vinegar beverage. The main sources of potential contamination microorganism were identified according to these results, which will help improving management to ensure the quality and safety of apple vinegar beverage.
  • ZHANG Yudan, LI Li, LIU Jun, LI Yuqin, LIU Qing
    Food and Fermentation Industries. 2019, 45(21): 201-206. https://doi.org/10.13995/j.cnki.11-1802/ts.021421
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    Sea-buckthorn sparkling wine was fermented by yeast using fresh Sea-buckthorn juice and rice saccharification solution as raw material. The fermentation parameters of Sea-buckthorn sparkling wine were optimized by single factor experiment and orthogonal test. During the single factor experiment, fermentation time, yeast inoculation, addition of rice saccharification solution, initial sugar content and fermentation temperature were investigated. The results showed that the best fermentation conditions were 20 g/L inoculation amount of yeast, adding 100 mL/L rice saccharification liquid, 18% initial sugar content and fermented at 25 ℃ for 80 h. The obtained sparkling wine had sour and sweet tastes, golden color, exquisite foam, flavor and aroma of Sea-buckthorn with an alcohol content of (5±0.5)% vol. Furthermore, volatile flavor components were analyzed by GC-MS/MS, twelve alcohols and ten esters were detected in Sea-buckthorn juice and Sea-buckthorn sparkling wine, among which ethanol, 3-methyl-1-butanol, benzyl alcohol and ethyl acetate, isopentyl acetate were detected in both samples. Ethyl acetate and ethyl caprylate were the main aroma component in juice and wine, respectively. This research lay the foundation for the development of sparkling wine products in the future.
  • ZHU Xiajian, TAN Anqun, FAN Jiaying, LI Zeling, ZHOU Qi, YI Xin, LI Guijie, GUO LI, TAN Xiang, HUANG Linhua, WANG Hua
    Food and Fermentation Industries. 2019, 45(21): 207-214. https://doi.org/10.13995/j.cnki.11-1802/ts.020813
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    This study was aimed to take full advantage of citrus resource with the increase of added value and reduced environment pollution. The fermenting condition of sweet orange pulp wine was optimized using the pulp residue from sweet orange after juicing, and the quality of fermented wine was compared with fruit juice. Sensory score, flavonoids and total phenols content were taken as the response values. The response surface analysis was carried out using Box-Behnken central combination design to establish a mathematical model and determine the optimal fermentation conditions. The optimal fermentation condition of 0.19% yeast inoculation at 30 ℃ and 23.5% initial sugar content resulted in 119.10 mg/L flavonoids content, 383.54 mg/L phenols content and sensory score of 80.12. Overall, wines fermented with sweet orange pulp residue had higher contents of phenol than those produced from juice, as well as strong flavor, good color and taste. It not only had typical citrus flavor, but also retained flavonoids and other nutrients in the pulp residue. The research has shown that pulp residue fermented wine can help improve the quality of sweet orange fruit wine and lay the foundation of industrial production.
  • TIAN Xin, ZHANG Huining, QI Xinchun, XU Yan, WANG Dong , TANG Ke
    Food and Fermentation Industries. 2019, 45(21): 215-220. https://doi.org/10.13995/j.cnki.11-1802/ts.018601
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    Twelve Cabernet Sauvignon dry red wines in four regions were evaluated by rapid sensory analysis technology-Napping® and Ultra Flash Profiling (UFP). The sensory data was processed by three multi-variate statistical analysis (Procrustes multiple factor analysis, Indscal model and cluster analysis). The results showed that: wines from different regions had obviously regional and similar aroma characteristics. Three kinds of multi-variate statistical analysis revealed that the Cabernet Sauvignon wines from Jinxi and Yantai had obviously regional characteristics. The wines from Jinxi presented black currant, red fruits and hawthorn odors. The wines from Yantai had significant smoky aroma characteristic. The sensory characteristic for wines from Ningxia and Xinjiang regions exhibited slight differences by different analytical methods. Procrustes multiple factor analysis (PMFA) and cluster analysis suggested that the difference between these two was small, while the Indscal model could distinguish better. In addition, the wines from Ningxia presented the odors of green peppers and sweet spices, while the wines from Xinjiang had more prominent baking, prune and floral aroma. The results indicated that the rapid sensory analysis technology can perform well on sensory evaluation of wine samples.
  • WU Yating, DU Muying, HE Huanhuan, KAN Jianquan, CHENG Fangfang, YIN Na, LIU Weibing, DING Chengyan, YIN Xiaoqing, WU Yun
    Food and Fermentation Industries. 2019, 45(21): 221-228. https://doi.org/10.13995/j.cnki.11-1802/ts.021646
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    This study was aimed to analyze the structural diverseness of bacterial and fungal communities in naturally fermented chili sauce from Hami (A), Yili (B), Changji (C), Aksu (D) and Urumqi (E) regions in Xinjiang by IlluminaMiseq high-throughput sequencing. 1 562 species of bacteria and 396 species of fungi were identified through operational taxonomic units (OUT). Among them, Streptophyta and Lactobacillus were the dominant bacteria in regions A, B, C and E. Streptococcus and Enterococcus were the dominant bacteria in region D. Furthermore. Pichia, Hanseniaspora, Wickerhammyces were the dominant fungi in regions A, B and C. The dominant fungus in regions D and E was Aspergillus. The specific strain of region A, C and E was Torulaspora, Clonostachys, Alternaria, respectively. This research reveals the microbial community divergence of natural fermented chili sauce from different regions in Xinjiang and also lay foundation for screening suitable fermented microorganisms of chili sauce.
  • BI Siyuan, CAO Jiantao, LI Baoling, ZHU Zhiqiang, CAO Tao, LI Sen
    Food and Fermentation Industries. 2019, 45(21): 229-235. https://doi.org/10.13995/j.cnki.11-1802/ts.020240
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    With 10 fish species in Xiamen area as the research objects, the residue dose and characteristics of organochlorine pesticides (OCPs) in fish were analyzed by QuEChERS-gas chromatography, and their possible health risks were discussed. Fish muscles were extracted with hexane and acetone (1∶1) homogenate and purified with 400 mg PSA, 150 mg C18 and 900 mg magnesium sulfate, followed by GC-ECD and GC-MS analysis. The results showed that the calibration curves were linear in the range of 1-50 ìg/L for each OCP, with correlation coefficients all above 0.998 8, the average recoveries of 83.2%-103.5%, the RSDs of 4.8-12.1%, and the detection limits of 0.01-0.09 μg/kg. Meanwhile, OCPs were detected in all 10 fish species, with the concentrations ranging from 5.532 μg/kg to 22.174 μg/kg. The concentrations of OCPs in Trichiurus lepturus and Ilisha elongata Bennett were the highest, reaching 22.174 μg/kg and 19.910 μg/kg, respectively. P′P′-DDD, P′P′-DDE and â-HCH were the main pollutants. The contents and composition of the isomers indicated that most of the OCPs in Xiamen area were come from early pesticide residues, but there was a suspected contamination of DDT caused by the degradation of dicofol. However, the EDI was far lower than daily food intake from the national standard. Therefore, QuEChERS can be applied to the detection of OCPs in marine fish, and the water environment in Xiamen sea area has been less polluted by OCPs recently, indicating low risk of OCPs by fish consumption.
  • CHENG Chuanxiang, WANG Pengxu, JIA Meng, ZHOU Xinzhi, MA Yaqin
    Food and Fermentation Industries. 2019, 45(21): 236-243. https://doi.org/10.13995/j.cnki.11-1802/ts.021429
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    The aim of this study was to analyze and identify the characteristic aroma substances of citrus juice from different citrus varieties and by different pressing methods as well as their contribution to the flavor, so as to provide a scientific-based reference for the evaluation and processing of citrus fruit quality. The aroma compounds in citrus juice were determined by gas chromatography-mass spectrometry(GC-MS) combined with multivariate statistical method. A total of 67 aroma substances were detected in 13 kinds of citrus juice, and 37 characteristic aroma substances were screened out. Among them, the aroma substances in loose-skinned citrus juice were lower than most of others, and the aroma substances in machine-pressed citrus juice were significantly higher than those in hand-pressed citrus juice. Multivariate statistical method could effectively distinguish citrus varieties and pressing methods. The correlation analysis showed that sweet aroma was positively correlated with ethyl octanoate and crotonate, green aroma with n-octanol and ethyl butyrate, and flower aroma with citronellol and valencene. GC-MS combined with multivariate statistical method is a reliable and effective method for identifying citrus varieties, evaluating the quality of citrus juice and distinguishing processing methods.
  • WANG Hongwei, SUO Huayi, ZHANG Yu, KAN Jianquan
    Food and Fermentation Industries. 2019, 45(21): 244-249. https://doi.org/10.13995/j.cnki.11-1802/ts.021083
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    The purpose of this study was to explore the effects of phenolic compounds on the smoked flavor and identify the key smoked odorants in Chinese bacon. Quantitative descriptive analysis was used to evaluate the odor characteristics of 6 kinds of smoked Chinese bacon and 2 kinds of non-smoked Chinese bacon. The volatile compounds of all bacon samples were identified by solid phase microextraction-gas chromatography-mass spectrometer (SPME-GC-MS). 4 Smoked flavor characteristics of 6 kinds of smoked Chinese bacon is indentified into smoky, woody, ashy and harsh. The smoked flavor characteristics were not found in non-smoked Chinese bacon. Six alkylphenols, such as phenol and six guaiacols were detected in smoked bacon samples, one smoked bacon possessed 3-10 kinds of phenolic compounds, but not found in non-smoked Chinese bacon. Partial least squares regression (PLSR) showed that all identified phenolic compounds except for 4-methoxyphenol, contributed to the smoked flavor characteristics of Chinese bacon. These results indicate that phenols are key indicator for the smoked flavor of Chinese bacon, which can provide theoretical basis for improving smoked flavor of food and developing smoke liquid.
  • CHEN Xiyu, JIAO Bining, ZHANG Yaohai
    Food and Fermentation Industries. 2019, 45(21): 250-257. https://doi.org/10.13995/j.cnki.11-1802/ts.020161
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    At present, the main international researches of carotenoids in citrus focus on orange juice, and lack of study on the detection and difference analysis of carotenoids in different kinds of loose-skin mandarins. The extraction conditions of carotenoids in citrus were optimized, followed by rapid analysis by ultra-performance liquid chromatography (UPLC). The varieties and contents of carotenoid compounds in pulps and peels were compared and discussed. The results showed that â-cryptoxanthin was the major carotenoid of the tested loose-skin mandarins, with total average content of 8.44 mg/kg FW in pulps and 23.73 mg/kg FW in peels. There was a notable difference of carotenoid contents and composition between 34 varieties, with the average content in pulps descending as follows: tangerines (14.73 mg/kg FW)>mandarins (13.51 mg/kg FW)>hybrids (8.11 mg/kg FW), while the order in peels was tangerines (47.94 mg/kg FW)>hybrids (28.71mg/kg FW)>mandarins (21.80 mg/kg FW). It is significant to make full use of the functional components in loose-skin mandarins.
  • FENG Xiya, HUANG Wei, SUO Huayi, WANG Hongwei, ZHANG Yu
    Food and Fermentation Industries. 2019, 45(21): 258-263. https://doi.org/10.13995/j.cnki.11-1802/ts.021299
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    In order to analyze the triglyceride composition and the physicochemical properties of peony seed oil, the crude oil of peony seeds was extracted by pressing method, and the basic physical and chemical indexes were determined by national standard method. The triglyceride of peony seed oil was purified by column chromatography, the fatty acid composition and triglyceride structure of peony seed oil were analyzed by gas chromatography and bovine pancreatic lipase hydrolysis, and the functional group structure was analyzed by Raman spectrum. The results showed that the basic physical and chemical indexes of peony seed crude oil met the requirements of national standard. There were 12 kinds of fatty acids in the crude oil of peony seed, mainly including linolenic acid (40.33±1.01 g/100 g), linoleic acid (21.08±0.61 g/100 g), oleic acid (15.67±0.59 g/100 g), palmic acid (7.59±0.46 g/100 g), and stearic acid (4.11±0.41 g/100 g). The total unsaturated fatty acid content reached 77.51±0.68 g/100 g. Furthermore, the relative content of triunsaturated fatty acid esters of triglycerides in the crude oil of peony seeds was the highest (79.68%), among which Sn-LOLn contributed the most (15.64%), followed by Sn-LnOLn (14.77%). In addition, Raman spectrum analysis showed that peony seed oil was an extremely unsaturated edible vegetable oil with almost no trans fatty acids. The results will provide a reference for the development and utilization of peony seed oil.
  • WANG Gang, ZHU Huiyue, YU Yunxia, ZHAO Jianxin, ZHANG Hao, CHEN Wei
    Food and Fermentation Industries. 2019, 45(21): 264-271. https://doi.org/10.13995/j.cnki.11-1802/ts.021579
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    Bacteriocin is a kind of antibacterial peptide synthesized by bacterial ribosomes, which is a hotspot in many fields such as food and medicine. Lactic acid bacteria (LAB) has been used in food for a long time and is generally considered to be a kind of safe bacteria promoting intestinal health. Therefore, bacteriocin, which can be synthesized by LAB, has great potential for practical application. In recent years, people's attention to intestinal flora has been increasing. With the wide application of LAB and the continuous exploration of its probiotic mechanism, the research on the influence of bacteriocins synthesized by LAB on gut microbiota shows great significance. Based on the relevant research progress, this review expounds the classification, characteristic, application of bacteriocin in LAB and its' influence on gut microbiota, in order to lay the foundation for the development and utilization of functional LAB and bacteriocin.
  • LI Hongjun, WANG Junpeng, HE Zhifei, LI Shaobo
    Food and Fermentation Industries. 2019, 45(21): 272-279. https://doi.org/10.13995/j.cnki.11-1802/ts.021194
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    The paper reviews theory of improving the quality and safety of animal derived foods as well as promoting the commercial development of intelligent packaging in the food processing industry. Based on extensive researches of domestic and international reports, the functional characteristics, operating principle, advantages and disadvantages of different types of intelligent packaging as well as the latest researches in the quality and safety monitoring of animal derived foods were systematically summarized. As a carrier of multi-nutritional factors, animal derived food is prone to deteriorate during storage and sale, which threatens the health and safety of consumers. Intelligent packaging can monitor the quality of food at different stages of the supply chain in real time, show the real shelf life, and ensure the safety and traceability. With the gradual maturity of intelligent packaging technology, intelligent packaging shows a promising prospect of development in improving the quality and safety of animal derived food, quantifying product quality, and reducing food waste in the future.