25 July 2017, Volume 43 Issue 7
    

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    Food and Fermentation Industries. 2017, 43(7): .
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  • WANG Kui,SU Ling-qia,WU Jing,CHEN Sheng
    Food and Fermentation Industries. 2017, 43(7): 1.
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    The production of trehalose from starch is an effective method under the combined action of maltosyltrehalose synthase (MTSase) and maltosyltrehalose hydrolase (MTHase).The optimum temperature of MTSase was 45℃,the optimal pH was 7.0,and the optimal temperature of MTHase was 55℃,the optimum pH was 6.0.Then trehalose was produced by the two enzymes together,and the reaction conditions were optimized.The effects of reaction temperature,initial pH,substrate DE value,enzymes amounts and substrate concentration on trehalose yield were investigated.The optimal conditions were as follows:the reaction temperature was 45 ℃,the initial pH was 8.5,the substrate DE value was 8.5 and the amount of MTSase and MTHase was 15.75 U/g and 7.5 U/g,respectively.
  • FU Xu-dong,WU Jian-rong,JIANG Yun,MA Xu,ZHENG Zhi-yong,ZHANG Li-min,ZHAN Xiao-bei
    Food and Fermentation Industries. 2017, 43(7): 7.
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    Polysialic acid (PSA) belongs to group II extracellular polysaccharide produced by Escherichia coli K235.Previous studies have shown that the accumulation of organic acids in the fermentation broth,pH control mode and K2HPO4 have a great influence on the biosynthesis and molecular weight of polysialic acid.In this study,wefound that the accumulation of organic acids and exogenous acetic acid addition reduced the production and molecular weight of polysialic acid.Compared with the 100 mmol/L K2HPO4 required for shake flask cultivation,20 mmol/L K2HPO4 could obtain higher molecular weight and yield of polysialic acid in fermenter.After 16 h of fermentation,the ammoniawater was switched to KOH feeding to control the pH,and the appropriate amount of tryptone was added as the organic nitrogen source.As a result,the production of polysialic acid was increased and the molecular weight and yield of PSA remained stable.Results from this study can provide a good hint for industrial scale production of PSA with stable molecular weight.
  • JIA Dong-xu,ZHOU Lin,WANG Teng,YU Chun-an,LIAO Cheng-jun,CHEN De-shui,WANG Hong-yan,LIAO Xiao-ying,JIN Li-qun
    Food and Fermentation Industries. 2017, 43(7): 13.
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    Thermophilic glucose isomerase (GI) can catalyze conversion of D-glucose to D-fructose at elevated temperature.The obtained high fructose corn syrup served as sweetener,is widely used in various food fields.Herein the fermentation and conversion conditions of E.coli BL21 (DE3)/pET28b/ToGI were studied.The results showed that the optimum fermentation should be performed at 28 ℃ by adding 0.1 mmol/L Isopropyl β-D-1-thiogalactopyranoside (IPTG) and 5 mmol/L Mn2+ Then,the amplified biotransformation reaction was conducted at the condition of 20 g/L wet cell,10 mmol/L Mn2+,200 g/L D-glucose and pH 7.5.After bioconversion at 95 ℃ for 4 h,the yield of D-fructose reached 57.34%.One-step production process of F55 high fructose corn syrup (HFCS) using this craft has the advantage of simplifying the subsequent step of enriching D-fructose,and it provided an important reference for industrial application of HFCS production using food safety expression system.
  • WANG Kun,LIANG Peng-juan,LI Shang-yong,WANG Wei,SUN Jing-jing,LIU Jun-zhong,SUN Mi,HAO Jian-hua
    Food and Fermentation Industries. 2017, 43(7): 20.
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    The alkaline metalloproteases MP is extracted from the strain YS-80-122 and belongs to the family of serralysin.Similarly to the other enzymes of this family reported before,there is an inhibitor gene lupI downstream of the MP gene and LupI can completely inhibit the activity of MP.On the basis of the wild-type inhibitor gene,four amino acids were added to the N-terminus of LupI,named LupI-MSSS.This work aims to construct a prokaryotic expression vector for LupI-MSSS,the recombinant plasmid was transformed to BL21 (DE3),and the expression was induced by IPTG.The recombination LupI-MSSS was separated using SDS-PAGE and the size of expressed LupI-MSSS was consistent with the prediction.Single factor experiment were used to optimize the fermentation condition and the optimal conditions were:the inoculation amount is 2 %,IPTG as the induced agent was added at 3 h after inoculating,and it~ final concentration is 0.5 mmol/L,the recombined E.coli need inducing 8 hours at 37 ℃.Consecutive steps were used to achieve the purified protein as follows:ultrafiltration,Q Sepharose ion exchange,Superdex 200 gel filtration,and the purity of LupI-MSSS is up to 99%.
  • LIU Dong-dong,ZHANG Wei-guo,XU Jian-zhong,TIAN Cheng-fu
    Food and Fermentation Industries. 2017, 43(7): 27.
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    Corynebacterium glutamicum (C.glutamicum) ZL-8 is an L-lysine producing strain of mutation breeding with low rate of glucose uptake and phosphorylation.Phosphoenolpyruvate-dependent glucosephospho-transferase system (PTSGGlc) and PTSGlc-independent play an important role in the process of glucose uptake and phosphorylation.In this paper,the roles of EIIGlc,IolT1,glucokinase in glucose metabolic rate,L-lysine yield and by-products accumulation were investigated by overexpressing EIIGLc (ptsG),IolT1 (iolTl),glucokinase (ppgk)coding genes in L-lysine producing strain Corynebacterium glutamicum ZL-8.Compared with the original strain,the L-lysine yield and glucose metabolic rate of the recombinant strain C.glutamicum ZL-8/pDXW-8-ptsG (increased by 27.3% and 16.4%,respectively) and C.glutamicurn ZL-8/pECXK-99E-ppgk-iolT1(increased by 16.7% and 24.7%,respectively) were increased by overexpressing the genes in glucose transport system.In addition,the activity of PTSGlc system in C.glutamicum ZL-8/pDXW-8-ptsG was increased by 62.7%,whereas the activity of glucokinase in C.glutamicum ZL-8/pECXK-99E-ppgk-iolT1 was increased by 768%.Meanwhile,results indicated that the accumulation of organic acids (pyruvic acid,lactic acid and acetic acid) and amino acids (valine,alanine,aspartic acid) changed more obviously during overexpression of the different genes involved in glucose transport system.
  • CHENG Xiu-li,QIN Hai-bin,XIONG Tao,NIU Kun
    Food and Fermentation Industries. 2017, 43(7): 35.
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    3-Hydroxypropionic acid (3-HP) is an important platform chemical that can be used to synthesize a range of chemical compounds.To improve 3-HP production,the E.coli W3110△yqhD (pCDFDuet-tac-gpdl-TUkgsadh/pACYCDuet-tac-dhaB1-4) was genetically modified by homologous recombination technology to knockout acetaldehyde dehydrogenase (yqhD).The recombinant strain produce 2.53 g/L 3-HP of flask culture,the production was increased 5.8 times compared with original one.On the other hand,the E.coli W3110△glpR (pCDFDuet-tac-gpdlTUkgsadh/pACYCDuet-tac-dhaB1-4) was genetically modified to knockout glycerol regulatory factor (named glpR).The recombinant strain produces 2.86 g/L 3-HP of flask culture,which was 6.7 times higher than that of the original strain.Finally,the strain of E.coli W3110? glpR (pCDFDuet-tac-gpd1-TUkgsadh/pACYCDuet-tac-dhaB1-4) could produce 15.4 g/L 3-HP in a 5-L fermentor.The results provide research basis for 3-HP production.
  • WANG Kang,LIU Liang-sen,CHEN Lei,ZHANG Wei-wen
    Food and Fermentation Industries. 2017, 43(7): 42.
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    Laboratory-based adaptive evolution was applied to increase the tolerance to ACCase inhibitor sethoxydimin Schizochytrium sp.S31.Through an experimental evolution process of continuous 65 passages for 200 days,the sethoxydim tolerance concentration in Schizochytrium sp.was increased from 200 pμmol/L to 500 μmol/L.After 96 h fermentation in shake flasks,a comparative analysis of wild type and evolved strains showed that the biomass of the final evolved strain ALE500 reached 14.7 g/L at 72 h,which was 20.2% higher than that of wild type.In addition,docosahexaenoic acid (DHA) yield was increased by 30.4% in the evolved strain compared with the wild type.Moreover,the unsaturation degree of total fatty acids was also significantly increased in the evolved strain ALE500 and reached 1.11.To further verify the domestication effect of evolved strain,fed-batch strategy was applied and 19.74% increase of biomass and 29.38% increase of DHA yield were observed.This study demonstrated that the application of the sethoxydim-tolerance increased the biomass accumulation and DHA production in Schizochytrium sp.S31.
  • YAN Yi-jiang,XIAO Yi-wen,WANG Ya,WU Wen-ting,CHANG Jun,HE Qiao-xuan,ZHU Du
    Food and Fermentation Industries. 2017, 43(7): 49.
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    A high-selectivity β-D-glucuronidase from Endophytic Fungus Chaetomium globosum S108 was immobilized into matrix with sodium alginate as embedding material and glutaraldehyde as crosslinking agent.The optimal immobilized conditions were achieved with 1.5% sodium alginate,1.5% glutaraldehyde,2% CaCl2 and 3 500 U of enzyme load of per milliliter matrix for 30 min.In addition,some immobilized enzymology properties were studied.Compared with free enzyme,the optimum temperature and pH of immobilized enzyme were higher than those of free form,and temperature stability and pH stability of immobilized enzyme were enhanced obviously.After immobilization of β-D-glucuronidase,the Michaelis constant (Km) was increased,and the maximum reaction velocity (Vmax) was decreased respectively.Immobilized β-D-glucuronicdase enzyme activity still retained 71% of its initial activity after consecutive 15 cycles.
  • QIU Ran,LU Jian
    Food and Fermentation Industries. 2017, 43(7): 55.
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    A total of 11 strains of beer-spoilage bacteria were isolated from draft beer.The homology of 16S rRNA was analyzed and phylogenetic tree was structured for these 11 beer-spoilage bacteria.These 11 beer-spoilage bacteria were all classified into Lactobacillus genus,including Lactobacillus plantarum,Lactobacillus parabuchneri and Lactobacillus brevis.Variation of beer-spoilage bacteria membrane lipid composition was consistent with the result of 16S rRNA.The contents of straight chain unsaturated fatty acids and C18 and C20 fatty acids of BN01,BN02 and BN03 were increased with increase of beer content.These results not only classified and identified beer-spoilage bacteria but also expound tolerance mechanism of beer environmental stress based on regulation of fatty acids of beer bacteria membrane.
  • BAO Jia-wei,XU Jian,SU Xian-feng,ZHANG Hong-jian,MAO Zhong-gui
    Food and Fermentation Industries. 2017, 43(7): 62.
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    To solve the potential extraction wastewater pollution in citric acid production,an integrated citric acid-methane fermentation system was proposed.In this system,the extraction wastewater was treated through mesophilic anaerobic digestion (AD) and then the anaerobic digestion effluent was further treated and recycled to make mash for the next batch of citric acid fermentation,thus the wastewater discharge was eliminated.Excessive Na + contained in AD effluent was confirmed to be the major inhibitor for citric acid fermentation and its concentration could accumulate to 1 000 mg/L in recycling.AD effluent was treated with electrodialysis system to remove sodium and then recycled to make mash for the next batch of the citric acid fermentation.The recycling was performed for 10 batches andthe average citric acid production was 142.4 ± 2.1 g/L,which was comparable to that of the fermentation with tap water (141.5 g/L).Effect of high concentration of sodium on the citric acid fermentation was investigated and the results indicated that 1 000 mg/L Na + contained in citric acid fermentation could decline the medium pH sharply at initial stage,which decreased the activity of isomaltase and glucoamylase secreted by Aspergillus niger,and influenced the breakdown of isomaltose and part of other dextrins,thus caused the decrease of citric acid production.18 g/L calcium carbonate was added to medium at the start of the citric acid fermentation,the decline rate of the medium pH slowed down,which could promote the breakdown of isomaltose and part of other dextrins,and thus could increase available sugar concentration and relieve the inhibition caused by 1 000 mg/L Na +.However,as the medium pH maintained at the high level because of the calcium carbonate addition,part of available sugar was converted to by-product oxalic acid and the citric acid production was 139.2 g/L,which was still lower than that of the fermentation with tap water.
  • LIU Fei,DU Li-ping,XIAO Dong-guang
    Food and Fermentation Industries. 2017, 43(7): 70.
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    Headspace solid-phase microextraction (HS-SPME) was commonly used for the extraction of volatile compounds.To determine the SPME fiber to extract volatile compounds in soy sauce,the sensitivity of different fibers were examined on the basis of cumulative area normalization value,and their repeatability were examined by the average value and standard deviation of relative standard deviation (RSD) of peak area.65μm PDMS/DVB was then selected to extract volatile compounds in soy sauce.By comparing the peak area of all kinds of volatiles and the total peak area,the extraction conditions were optimized,and the extraction conditions were established as follows:the volume of soy sauce sample was 8 mL,extraction temperature was 60 ℃C and extraction time was 60 min.Using the optimized method for the extraction of volatile compounds in soy sauce,a total of 86 compounds were identified by GCMS,wherein the main compounds were esters and followed by alcohols,aldehydes and acids.The average RSD of the peak area of compounds was 8.14%,which showed that the repeatability of the method was well.
  • XU Yong-xia,ZHAO Jia-mei,LI Tao,ZHOU Xiao,LI Hao-yu,SUN Tong,LI Jian-rong
    Food and Fermentation Industries. 2017, 43(7): 76.
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    To investigate of controlled release of volatile flavor compounds in garlic oil in different gel complex,the changes of flavor compounds in garlic oil with different gel complex during storage were analyzed by solid-phase micro-extraction (SPME) combined with gas chromatography-mass spectrometry (GC-MS).The release effects of different gel complex on the flavor compounds in garlic oil were analyzed by flow test.The result showed that the major volatile flavor compounds in garlic oil-gel were sulfur-containing compounds,alkenes,alcohols,ethers and aldehydes.The relative content of diallyl disulfide was the highest among the identified sulfoether compounds.Gelatin/ pectin,gelatin/xanthan,gelatin/Arabic gum.Gelatin gel had significantly binding and slowly release effect on major volatile flavor compounds in garlic oil compared with the control.The complex gel was better than single gel in controlling the flavor release.Among all gel complex,Gelatin/Arabic gum was the best;followed by gelatin/pectin,gelatin/xanthan and the single gelatin was the least effective.The differences in viscosity of different gel complex caused the differences in releasing the volatile compounds.
  • ZHAO Hong-yu,XU Wei-zhen,YANG Guo-hua,LIU Yuan-fu,YUE Peng,ZHANG Liang
    Food and Fermentation Industries. 2017, 43(7): 81.
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    To understand the changes of sensory and physicochemical properties and nutritional quality of Pixian Soybean Paste(PSP) during late ripening,sensory,physical and chemical properties as well as nutrients,such as Ca、Mg、Cu、Mn、Zn、Fe six trace elements were tested in 5 different PSPs samples at the different time periods.The results showed that the sensory qualities and physicochemical properties of PSPs at different after-ripening periods were significantly different.The water contents,capsicum red pigment and pH value in PSP decreased,amino acid nitrogen and total acid kept increasing with the extending of the ripening time.All six trace elements in PSP continued to increase and remained stable after 2-3 years of post-fermentation.Post-fermentation from 2 to 3 years is an important transitional stage.The amino acid nitrogen and 6 trace elements had no significantly differences in the samples that had been post-fermented for more than 3 years.The sensory qualities were decreased with the extension of after-ripening fermentation.The results provided data for determining the time of after-ripening fermentation of PSP,and laid a foundation for the further improvment of traditional PSP industry.
  • SUN Li-jie,ZHANG Hui,WANG Li,QIAN Hai-feng,QI Xi-guang
    Food and Fermentation Industries. 2017, 43(7): 87.
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    In this study,the thermal hysteresis activity (THA) was used as the main parameter to determine the optimum protease and hydrolysis time for salmon skin gelatin hydrolysis.The effects of peptides on biological antifreeze activity and thermal properties of frozen dough were investigated.The results showed the antifreeze peptide with maximal THA was obtained from papain treated gelatin,and hydrolysis at pH 6.0 for 2 h at 40 ℃.The substrate concentration was 3% (w/v) and papain proportion was 3 000 U/g.The THA was the highest of 0.53 ℃ under the above conditions.Their molecular weight were mainly distributed below 3 000 Da.When the concentration of SaAFP was 20 mg/mL,the survival rate of Escherichia coli was up to 75.71%.Differential scanning calorimeter measurement showed that SaAFP did not decreased the freezing temperature (Ti),melting temperature (Tm) and the freezable water content (Fw),but narrowed down the melting temperature (Tm,δ) of fresh dough.The SaAFP significantly lowered Fw of frozen dough after freeze-thaw cycles.
  • KAN Wen-han,JI Xiao-wen,WANG Yu-ting,MEI Lin,WANG Zhi-geng
    Food and Fermentation Industries. 2017, 43(7): 93.
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    Casein Phosphopeptides (Casein Phosphopeptides,referred to as CPPs) is a class of polypeptides which has divalent metal chelating activity.The theory and practical significance of CPPs is its specific nutrient chelated.CPPs was obtained by enzymatic hydrolysis,and separated Q strong anion exchange chromatography and reverse phase preparative chromatography (RP-HPLC);it had highly active zinc chelate peptide(CPP-Zn).Its structural was analyzed by proportion of amino acids、UV-VIS spectra、infrared spectroscopy、13C NMR spectra、1H NMR spectra and 31p NMR spectra.Result:CPP purified from continuous chromatographic separation had strong zinc chelating ability of 88.68ug / mg.Structure analysis showed CPP structure and proportion of amino acids before and after chelation significantly changed.The phosphate groups are involved in the chelation,-COOH、-NH2 and-OH in P-OH were the primary binding site.
  • SHI Fang,XIAO Xing-ning,LI Yao,YANG Ya-xuan,GUO Xiao-hui,WU Su-rui,MING Jian
    Food and Fermentation Industries. 2017, 43(7): 98.
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    The moisture distribution and change in seven kinds of edible fungi (Agrocybe aegerita,Pleurotus eryngii,Flammulina velutiper,Agaricus bisporus,Hypsizygus marmoreus,Lentinus edodes,Floral mushroom) during constant temperature drying process was analyzed by low-field nuclear magnetic resonance technology.The spin-spin relaxation test showed that the water states in edible fungi included free water,immobilized water and bound water.The free water and immobilized water was decreased significantly during drying process,while the bound water was not significant.Further found that the internal water distribution of seven kinds of edible fungi were uneven by using nuclear magnetic resonance imaging technique.The water loss rate of different edible fungi was not the same in the same stage.LF-NMR technology successfully realized the online monitoring of internal moisture of edible fungi during drying process,which provided theoretical basis for guiding the drying process of edible fungi.
  • XIAO Qin-lin,TAN Jiu-ming,QIN Xiao-li,ZHONG Jin-feng
    Food and Fermentation Industries. 2017, 43(7): 105.
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    This paper studied different factors such as ultrasonic temperature,ultrasonic time,liquid-to-material ratio and ultrasonic power on the extraction rate.A prediction model (E =1.023 × t0.596 × ln(13.553 × L × e 1027.514 + 1)) was established.The linear correlation coefficient between the experimental value and predicted value (R2) was 0.955 4,which suggested the model could effectively predict the changes in protein extraction rate.The results can provide some guidance for further research on protein extraction.
  • LIU Min,DAI Yao-yi,BI Jia-yu,ZHANG Fu-sheng,ZHENG Jiong
    Food and Fermentation Industries. 2017, 43(7): 109.
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    In order to explore the effect of hydrophilic colloid on properties of starch,the pasting,rheological,textural properties and microstructure of lotus root starch by adding konjac gum were investigated.The results showed that the viscosity,setback and breakdown values of lotus root starch were improved.The mixed system was pseudoplastic fluid,the consistency coefficient K was increased but the fluid index n was decreased,the mixed system exhibited a more significant thickening effect.The viscosity ratio was increased after adding konjac gum,the mixed systems had excellent visco-elastic characteristic,konjac gum accelerated the retrodegradation of lotus root starch.The hardness,elasticity and cohesion of starch mixed systems were decreased,but the adhesion of the mixed systems was increased;when the lotus root starch and konjac gum mass ratio was 8.5∶ 1.5,the gel was the softest.Meanwhile,a more uniform and compact network structure was observed by scanning electron microscopy.
  • ZHAO Tian-tian,ZHAO Dan,MA Xiao-han,DENG Jing,TIAN Jun-qing,LIU Xiong
    Food and Fermentation Industries. 2017, 43(7): 115.
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    different amount of inulin on the rheological properties of dough and bread baking quality were studied.The results showed farinograph properties,extensograph properties and other rheological properties of bread flour were changed significantly by inulin.With the increase of inulin,dough water absorption rate significantly dropped,dough development the time and dough stability were extended,and the dough weakening was reduced;the elastic and extensibility and maximum resistance of dough,were all increased;elastic modules and viscosity modules significantly increased,the dough network structure improved.However,the volume of dough after fermentation was decreased,bread specific volume rate reduced and initial hardness increased significantly.However,the hardness of the bread with 2.5% 、5% 、and 7.5% inulin was still much lower than that of without inulin during the storage time.The results showed that adding a certain amount of inulin can reduce the bread hardness,improve the dough internal structure,delay the aging speed and prolong the bread shelf life.
  • ZHAI Lei,LING Kong,SONG Zhen,YAO Su,CHENG Chi,YANG Yuxin
    Food and Fermentation Industries. 2017, 43(7): 122.
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    Eleven lactic acid bacteria were isolated from traditional fermentation foods including camel milk,mare milk and cheese in Kazak by culture-dependent methods.These isolates were identified by 16S rRNA gene and pheS gene sequences phylogenetic analysis,combined with morphology and physiological and biochemical characteristics.The abilities of acid-and salt-resistance,acid production,nitrite degradation,amino acids decarboxylases and antimicrobial properties were detected to screen lactic acid bacteria with potential applications.As a result,three lactic acid bacteria designated as L.paracasei KCH3 (CICC 6277),L.fermentum KM1 (CICC 6278) and L.fermentum KC3 (CICC 6290) were obtained and laid a foundation for vegetable fermentation application.
  • XIRINAY · Ablikim,MUYESSER · Yusup,NURGUL · Rahman
    Food and Fermentation Industries. 2017, 43(7): 128.
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    To explore the Lactic acid bacteria diversity and biofilm formation in traditional fermented milk from Xinjiang longevity region,the samples were analyzed using pure culture and 16S rRNA gene sequence analysis,96 well micro-plate semi-quantitative methods and scanning electron microscopy.Finally 57 strains were isolated from four samples.The micro-plate semi-quantitative method was used to investigate biofilm formation of strains.The resuits showed that 38 strains (73.68%) of the LAB showed biofilm formation ability,of which 16 strains (28.07%) strongly adhered to biofilm and 22strains (38.59%) were moderately adherent.The strains which can form biofilm were belonging to three species of six genera including Enterococcus durans (16 strains),Enterococcusffaecium (1 strains),Enterococcusthailandicus (7 strains),Enterococcus lactis (3 strains),Lactobacillus plantarum (6 strains),Streptococcus thermophilius (5strains),in which Enterococcus was the dominant species and was followed by Lactobacillus and Streptococcus.These provided foundation for the development and utilization of LAB resources and their biofilm characteristics.
  • MA Wen-rui,WEI Yu-jie,ZOU Wan,WU Hao-tian,TIAN Ge,WANG De-liang,MA Jing,GAO Lin-long,WU Yun,XUE Jie
    Food and Fermentation Industries. 2017, 43(7): 134.
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    39 yeast strains from three major wine-producing regions in Xinjiang including Changji,Manasi and Fukang were screened out to determine culture medium formulation and sensitivity of organic acids high-yielding Saccharomyces cerevisiae.Through initial and repeated screening,t strain of Saccharomyces cerevisiae with high-yielding acids named as Y6 was screend out.The results showed that the volatile acid content of Saccharomyces cerevisiae Y6 was the same as that of commercial yeast strain 71B.Based on these single factor tests,response surface methodology with three variables was employed to optimize the fermentation conditions such as initial acidity,fermentation temperature and inoculum amount.Results showed the optimal fermentation conditions were grape juice initial acidity of 6.70 g/L,fermentation temperature of 20.35 ℃ and inoculum amount of 109 CFU/mL.Under these optimal conditions,the acid production capacity reached 1.766 4 g/L.Finally,the consistent results between the predicted value and actual value indicated that the established model in this study was feasible.In a small scale of wine fermentation experiment,compared with the commercial strain 71 B,the acid production of Y6 was increased by 1.84 g/L.
  • ZHOU Xiao-xin,LIU Peng,LI Chao,ZHUANG Ying-ping,CHU Ju,LI Liang,YANG Wen-jing,KUANG Jin-bao
    Food and Fermentation Industries. 2017, 43(7): 141.
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    In this paper,the trehalose accumulation conditions and cell yield of baker's yeast were studied.The results showed that the limitation of carbon source in the early stage of fermentation did not cause the accumulation of trehalose in advance.Dissolved oxygen limit in the later stage of fermentation could increase the cell yield without reducing the trehalose content.Based on the results of this study,a novel semi-continuous fermentation process was proposed.The average production efficiency was increased to 3.7 g/(L · h),which was 62.7% higher than that of the original process,which provided a new idea for efficient production of highly active yeast.
  • WU De-zhi,GUO Wei,GAN Gui-fang,WU Meng,LI Xiao-hong
    Food and Fermentation Industries. 2017, 43(7): 146.
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    Pueraria residue was fermented by Lactobacillus bugaicus and Streptococcus thermophilus (ratio 1 ∶ 1) to prepared dietary fiber (DF).On the basis of single factor experiment,effects of fermentation temperature,time and amount of inoculum were studied via Box-Behnken response surface methodology.And then the formula optimization of biscuit was determined by Box-Behnken response surface methodology.Results showed that the optimized extraction parameters were fermentation temperature 35 ℃,fermentation time 23.5 h and inoculum 5%.Under the conditions,the yield of prepared DF was 79.20.21%.The water holding capacity,swelling capacity and water binding capacity of the prepared DF were 7.260.13 g/g,5.790.06 mL/g,5.820.15 g/g,respectively.The optimized formula of biscuits was confirmed to be as followings:DF addition was 6%,oil addition was 30 % and ratio of baking soda to ammonium bicarbonate was 1.8.After P.praecox dregs fermentation,the purity and physicochemical properties of the DF were improved,thus indicated that this fermentation method was feasible.Moreover,DF could significantly enhance the hardness of biscuit.This study provided reference for the development of Pueraria residue.
  • YAN Yan-ying,WANG Xi,FAN Xue-hui,ZHANG Qing-an
    Food and Fermentation Industries. 2017, 43(7): 152.
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    Studies have shown that ultrasound can induce accelerated aging of wine,which is the most important problem for the black rice wine enterprise,but the effect of ultrasound on organic acids of black rice wine has not been reported.In this paper,the contents of 5 kinds of organic acids in black rice wine were measured by high performance liquid chromatography (HPLC) under different ultrasonic parameters (power,frequency and time).The results showed that the content of tartaric acid and citric acid decreased,and the content of oxalic acid,lactic acid and acetic acid increased under ultrasound irradiation.These results will provide a useful reference and theoretical basis for the further optimization of ultrasonic parameters in black rice wine,and for the early application of ultrasound in the process of black rice wine aging.
  • CHE Chang-yuan,DONG Xue,ZHANG Qin-fang,WANG Shu,HU Xiao,LI Jie,DONG Qing-ling,SUN Shu-yang,ZHAO Yu-ping
    Food and Fermentation Industries. 2017, 43(7): 157.
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    Malolactic fermentation (MLF) is vital for cherry wine production,since it can reduce the wine's acidity,improve the mouth feel,enhance the biological stability and improve the wine' s flavor complexity.In the present study,three commercial Oenococcus oeni strains were used as candidates to compare their evolutions during MLF and their influences on the compositional profile,volatile profile and biogenic amine contents of cherry wines.Obtained results demonstrated that all the MLF processes involving the three O.oeni strains were finished within 20 days,and no significant differences or only slight differences were observed in the basic composition and biogenic amine levels among these cherry wines.Moreover,Lalvin VP41 was found to significantly boost the production of several volatile compounds in cherry wines derived from this strain,such as 2-phenylethanol,ethyl butyrate,3-methylbutyl acetate and α-terpineol,and therefore reinforced the wine' s fruity and floral characters.Based on above findings,LalvinVP41 was selected as the most suitable O.oeni strain for the production of cherry wines.
  • LI Mei,GONG Jia,XIAO Mei,WU Yue,CUI Yang,BU Meng-jun,SU Man-wen,HU Yong-zheng,LI Ming-yuan,DING Wen-wu
    Food and Fermentation Industries. 2017, 43(7): 163.
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    The contents of main nutrients of bamboo shoots (Phyllostachys eduis) in the fermentation process with different treatments were analyzed and compared to provide theoretical basis for preservation of fresh bamboo shoots.The results showed that the effect of blanching was the best for preservation of bamboo shoots compared with the other three treatments.After 63 d of fermentation,the soluble sugar,cellulose and protein in the bamboo shoots were 0.13%,8.87% and 0.36/100 (g/g),respectively.The contents of potassium and calcium respectively increased 458.48mg/kg and 50.53mg/kg while the losses of iron and magnesium were the least.Compared with the other three methods,the treatment of blanching and fermentation could keep the contents of soluble sugar and protein of bamboo shoots better,reduce the rate of lignification and accelerate the element transfer between the bamboo shoot and the broth,which was beneficial for increasing the contents of elements in the bamboo shoots.These results indicated that the edible quality of bamboo shoots could be kept effectively while the bamboo shoots could be preserved longer by this method.
  • YU Yu-le,HE Zhi-fei,LI Hong-jun
    Food and Fermentation Industries. 2017, 43(7): 169.
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    The protein isolate extracted from Ira rabbit meat by pH-shifiing method was studied.The result showed that the optimum conditions for acid-soluble and alkali-soluble were pH 3.0 and pH 12.0.The optimum recovery condition was pH 5.5.The yield of rabbit protein isolate were 46.46% by acid method and 68.55% by alkali solution.The protein content (dry basis) of acid method and alkali method were both significantly higher than of rabbit meat,and fat content (dry basis) were both significantly lower than that of rabbit meat.Besides,a variety of essential amino acids of protein isolates were higher than FAO/WHO recommend value.SDS-PAGE pattern indicated no obvious protein degradation was found in the protein isolates extracted by pH-shifting method.
  • QI Ting,CHEN Xue-zhong,LIU Zhi-dong,HUANG Hong-liang,WANG Yi-hong,LIU Bao-lin,QU Ying-hong,WANG Wen-han,FENG Shi,SHI Yan-guo
    Food and Fermentation Industries. 2017, 43(7): 174.
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    The effect of ultrasonic technology on the protein properties of Euphausia superba was studied.Through single factor experiments,the effects of ultrasound power,ultrasound time,ultrasound temperature on protein water absorption,oil absorption and solubility were studied.The result showed that ultrasonic treatment time has obvious effect on water absorption and solubility,and certain degree to emulsification.Ultrasound can changed the protein properties.
  • GUO Sha-sha,CHEN Yi-lun,YAO Qiao-yun,PENG Xiao
    Food and Fermentation Industries. 2017, 43(7): 181.
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    Ascorbic acid,sodium ascorbate,isoascorbic acid and sodium isoascorbate were added into fresh pork before cooking.The changes of nitrite,N-nitrosamine,red value and lipid oxidation were studied.Effect of antioxidant on nitrite conversion pathway was measured by the contents of nitric oxide,nitrate and nitrite.The result showed that the antioxidants could significantly reduce residual content of nitrite and N-nitrosamine content,improve the red value and delay lipid oxidation.Sodium isoascorbate was the best.When the dosage of sodium isoascorbate was 500 mg/kg,the residual content of nitrite was 26.24 mg/kg,the degradation rate was 72.49%,N-nitrosamine content was 1.05 μg/kg,decreased 69.39%,a* value increased 13.49%,TBARs decreased 60.00%.After adding 500 mg/kg sodium isoascorbate,the nitrite content in the disproportionation reaction decreased from 27% to 17%,the nitrite content in the degradation reaction increased from 9% to 65%,and the residual content decreased from 64% to 18%.The results showed that sodium isoascorbate could inhibit nitrite disproportionation reaction,but significantly promote the degradation reaction,resulting in a significant decrease in the content of residual nitrite.
  • ZHANG Li,ZHANG Lei,LUO Li-yong,PAN Cong-fei,ZENG Liang
    Food and Fermentation Industries. 2017, 43(7): 186.
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    The effects of baking techniques on the volatile components and aroma characteristics of Wuyi mountain tea were studied.Two cultivars of named Shuixian and Rougui were processed by different baking techniques.Then aroma components of raw sample and baked samples were studied using gas chromatography-mass spectrometry (GC-MS) combined with tea sensory evaluation.The results showed that 88 kinds of aroma components were detected,including 14 kinds of alcohols,14 nitrogen compounds,7 hydrocarbon compounds,19 esters,18 aldehydes,12 ketones,1 acid,2 heterocyclic oxygen compounds and 1 sulfur compound.Alcohols,nitrogen compounds and aldehydes accounted are the most with the content of 35.58%,20.28% and 19.25%,respectively.Besides,as the baking degree increased,the alcohols decreased while the esters and aldehydes were increased.Notably,the substances with an aroma of flowery and fruity such as dehydrogenation linalool,cis-3-hexenyl hexanoate,hexyl hexanoate increased first then decreased,and substances with an aroma of baked and caramel of 1-ethyl-1 H-pyrrole showed an increasing trend while benzyl cyanide,2,5-dimethylpyrazine,2-ethyl-5-methyl pyrazine and 2-acetylfuran increased first then decreased.Additionally,the aroma components of Wuyi rock tea made from Shuixian and Rougui were significantly different.The aroma components of Shuixian were mainly alcohols and nitrogen compounds,while the main aroma in Rougui were alcohols and aldehydes.Moreover,with deep roasting,the changing pattern of the main aroma components,such as nitrogen compounds,aldehydes,nerolidol,linalool,geraniol,in Shuixian and Rougui were significantly different.According to the sensory evaluation,Shuixian at roasting degree 3 and Rougui at roasting degree 2 had better aroma quality,and a heavily-baked aroma were appeared in tea at roasting degree 4 and 5.
  • HUANG Xiao-li,XIAO Xu-lin,XU Wei,XIE Xing,GUO Yu-rong
    Food and Fermentation Industries. 2017, 43(7): 194.
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    There are a series problems for conventional apple pomace drying,such as,long drying time,high energy consumption,severe browning.Combined drying method was used in this study.Superheated steam drying was used first when moisture content from 81% to 63%,and then hot air drying was applied to continue reduce the moisture content from about 63% to 7%.In order to study the superheated steam drying characteristics of apple pomace,two-factor,four-level orthogonal experimental scheme was designed using steam temperature and velocity.The hot air drying condition was set at 70℃,1.5m/s.Results showed that the drying process had condensation and constant drying periods,and the dehydration rate of apple pomace was increased with the increase of steam temperature.The velocity of superheated steam had little effect on the drying.With superheated steam,the hot air drying time was shortened remarkably.The color values a and b of the apple pomace powder dried with combined drying method were lower,which means the browning of dried apple pomace powder was getting better.The water holding capacity,oil holding capacity and average pectin yield ratio of dried apple pomace powder were 4.82 to 7.77,0.83 to 1.32 and 9.3%,respectively.Compared to single hot air drying,superheated steam combined with hot air drying can successfully reduce the drying time and the browning of apple pomace,and increase pectin yield ratio.These results can be used as a guide in reuse of apple pomace.
  • YANG Lin-xin,WANG Bing-bing,YIN Ya-lan,JIN Yin-zhe,CHENG Yu-dong
    Food and Fermentation Industries. 2017, 43(7): 201.
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    The breaking force,gel-formation rate,activation energy and absorbed energy of unit mass were chosen as indexes to analyze the gel formation mechanism of minced white shrimp (Penaeus vanmamei) by different heating processing.The effect of addition of soy protein isolate (SPI) emulsion on gel properties were investigated during heating.Results indicate that breaking force of minced shrimp and SPI compounds increased with the increase of heating temperature,and the addition of soy protein isolate emulsion could improve breaking force.Compared with waterbath and microwave heating processing,the gel-formation rate of single minced shrimp by microwave heating was much higher than that by water-bath heating,and the breaking force introduced by microwave heating was higher than water-bath heating when these samples absorbed the similar energy.Moreover,the activation energy of SPI compounds was significantly higher (P < 0.05) than the single minced shrimp,and the gel formation rate reduced slightly with the increase of SPI emulsions.These results indicated that the microwave heating was more appropriate in the industrial development of gelation foods.
  • CHEN Shuang,XU Yan
    Food and Fermentation Industries. 2017, 43(7): 207.
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    The volatile compounds in Chinese roasted sesame-like flavor type liquor were characterized by headspace solid-phase microextraction coupled with comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry (HS-SPME-GC × GC-OFMS).Ordered chromatograms of homologous series in sesame-like flavor type liquor are observed using the two-dimensional gas chromatography system.More than 1 000 peaks were separated and detected by HS-SPME-GC × GC-TOFMS.Among them,340 volatile compounds were further identified by different strategies,including 180 esters,26 alcohols,15 acids,88 aldehydes and ketones,16 nitrogen Containing Compounds,20 furans,25 sulfur Containing Compounds,14 terpenoid compounds and 6 others.Eleven sulfur Containing Compounds and 12 terpenoid compounds were identified in roasted sesame-like flavor type liquor for the first time.
  • ZHANG Yu-wei,JING Meng-lin,LI Xiao-ping,WEI Si-fan,HU Xin-zhong,MA Zhen,LIU Liu
    Food and Fermentation Industries. 2017, 43(7): 214.
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    In order to evaluate the comprehensive qualities of protein and amino acids in different varieties of buckwheat and their sprouts,and study the effects of germination on protein and amino acid of buckwheat,the protein and amino acids content of 10 varieties of buckwheat (5 kinds of sweet buckwheat,5 kinds of tartarian buckwheat) and their sprouts were determined by Kjeldahl Nitrogen Determination and automatic amino acid analyzer.The correlations were analyzed by principal component analysis and systematic cluster analysis.The results showed that buckwheat seeds and their sprouts were rich in glutamic acid,arginine,lysine,alanine and aspartate amino acids.Germination process can significantly improve protein content (average growth of 50.0%),total amino acid (average growth of 37.0%),total essential amino acid (average growth of 43.7%),and most of amino acids.Three principal components were extracted,and principal component analysis of amino acids of buckwheat and comprehensive evaluation model was established,which is F =F1 × 0.766 1 + F2 × 0.103 4 + F3 × 0.066 6.The comprehensive scores of amino acids were calculated by the model for the overall quality of amino acids of buckwheat.The correlation coefficient between the composite scores and the content of total amino acids and essential amino acid were 0.974 1 and 0.990 9 respectively.Combining with clustering analysis,comprehensive evaluation results showed that the sprouts of Chifeng 2,Haizige tartarian buckwheat and Hunan 7-2 had a better quality.
  • JIANG Hong,PANG Xiang-dong,QIN Ai,XIE Qiu-xia
    Food and Fermentation Industries. 2017, 43(7): 222.
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    A new acid fuschin resonance light scattering method for the determination of toxic heavy metal element mercury in canned meat was established.The spectrum characteristics of resonance light scattering and optimum reaction conditions and the effects of coexistent substances and reaction mechanism of ternary complex were discussed.In the Tris-Hydrochloric acid buffer medium of pH 6.56,cetylpyridine bromide sensitization acid fuschin were combined with mercury(Ⅱ) to form ternary complexes,which distinctly enhanced the resonance light scattering (RLS) of the system and created a new resonance light scattering spectrum.In the vicinity of the maximum resonance light scattering peak,the absolute value of resonance light scattering enhance intensity (| △IRLS |) was directly proportion to the mass concentration of mercury(Ⅱ) in the range of 0.005 to 0.40 mg/L with quantitative limitation of 0.032 mg/kg.The method was simple and sensitive,the standard addition recovery and RSD% (n =5) were in the ranges of 98.5%-102% and 2.0%-2.5% respectively.The method is suitable for the determination of mercury in canned meat.
  • MA Xin-ying,ZHU Qi,ZHANG He-ping
    Food and Fermentation Industries. 2017, 43(7): 227.
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    Poly melamine film modified glassy carbon electrode (PMel/GCE) was fabricated by electroplymerization of melamine.The electrochemical behavior of antioxidants tert-butylhydroquinone (TBHQ) was investigated by cyclic voltammetry (CV),a couple of sensitive redox peaks obtained.It was found that the oxidation peak current (ipa) and the reductive peak (ipc) current at PMel/GCE were obviously stronger than those at the bare glassy carbon electrode(GCE),which indicated that the sensitivity for detection of TBHQ was improved.Experimental parameters,including electro-polymerized time of Mel,scan rate,voltage,cycles and pH value of buffer solution were optimized.Under the optimal conditions,good liner relationships between current intensities and TBHQ concentrations were obtained in the ranges of 4.00 × 10-7-1.00 × 10-4 mol/L,with correlation coefficients (R) of 0.997 5.The detection limit was 2.0 × 10-9 mol/L.The modified electrode can be applied to the analysis of TBHQ in food with recoveries of 96.9%-102.2%.
  • SHAN Zhan,FAN Jing-jing,MA Xiao-long,TANG Ning-li
    Food and Fermentation Industries. 2017, 43(7): 233.
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    In HAc solution,tetracycline(Tc) reacted with Cu2+ to form cation chelates,which further reacted with chromeazurol S to form a ternary ion-association complexes.This enhanced resonance scattering signals.A new resonance light scattering method was established for the determination of trace Tc.Under the optimal conditions,the increase of the intensity(△I) at 700nm was linear to concentration of Tc in the range of 0.2-22 μmol/L.Its regression equation was △I =1.021 × 107c + 1.437,with a correlation coefficient of 0.999 0 and a detection limit of 8.81 × 10-9 mol/L.This method was applied in determination of Tc in milk samples with satisfactory results.The recovery was within the range of 100.00%-101.20%.
  • GU Dong-chen,ZHANG Long,YUAN Kai,CHEN Xi-feng,WANG Xi-chang
    Food and Fermentation Industries. 2017, 43(7): 237.
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    To establish a rapid and quantitative detection method of formaldehyde in squid,electric nose was used for screening massive samples.The results showed that the identification for volatile compounds in squid with different formaldehyde content were mainly depended on eight sensors of electronic nose.After PCA data analysis,DFA and SIMCA were used to establish a discrimination model for detecting formaldehyde in squid,and the feasibility of using the model in rapid determine formaldehyde was tested.Finally,a rapid quantitative prediction model by using PLS was established (R2 =0.926 6).To verify the accuracy of the quantitative model,t-test was employed and no significant difference between predicted values and true values.This study showed the method has a good potential in fast,nondestructive and quantitative detection of formaldehyde in food.
  • LIU Zhi-dong,WANG Lu-min,CHEN Xue-zhong,WANG Yi-hong,HUANG Hong-liang,QU Ying-hong,WANG Wen-hang,MA Qing-bao,QI Ting,YAN Meng-ya
    Food and Fermentation Industries. 2017, 43(7): 242.
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    Antarctic krill (Euphausia superb Dana) plays a central role in the Antarctic waters ecosystem.Protein derived from Antarctic krill has attracted much attention to food,pharmaceutical,nutraceutical,cosmeceutical scientists due to its abundant resources,good nutrition properties,functional properties and potential applicable values.This paper reviews the chemical composition,extraction and isolation techniques,nutrition,safety and functional properties of protein derived from Antarctic krill;it also introduces the present situation and exiting main problems of the processing and utilization of protein derived from Antarctic krill in domestic and abroad.The advances of research were summarized.
  • LI Shuang-hong,YE Fa-yin,LEI Lin,ZHAO Guo-hua
    Food and Fermentation Industries. 2017, 43(7): 252.
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    Amphiphilic polysaccharides is a class of semisynthetic polymer with low toxicity,good biocompatibility and degradable.It can form self-assembled hydrophobic-hydrophilic core-shell structure in aqueous.Recently,this kind of micelles got more attention in the field of food science,medicine,biomedical engineering and even material science.The polysaccharide-based micelles research is mainly focused on improving the bioactivity of hydrophobic compounds.Based on the literatures review of the last five years,this paper reviews the applications of amphiphilic polysaccharide-based micelles on targeted delivery,scattered solubilization,delayed release,as well as bioavailability of hydrophobic substances.Finally,the existing problems and future development in this field are also discussed.
  • ZHAO Sheng-ming,ZHAO Yan-yan,MA Han-jun,BIE Xiao-mei
    Food and Fermentation Industries. 2017, 43(7): 259.
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    Since microorganism is one of the most primary factors to cause food spoilage and foodborne disease,the inhibition of the growth of microorganism is an important research direction in food and medical field.Therefore,the study on antimicrobial mechanism is a hot topic in domestic and aboard academe.The utilization of transcriptomics could reveal the molecular mechanism of various antimicrobial substances against microorganism.The main research methods for transcriptomics,especially the technical features of the most widelyused RNA sequencing technology were reviewed in this paper.The research situations of transcriptomics for antimicrobial mechanism at home and aboard were introduced.With the development of high-throughput sequencing technology,transcriptomics will have extensive application prospects in research of antimicrobial mechanism.
  • WANG Xin-yue,LI Hong-jun,LI Shao-bo,GAN Xiao,HE Zhi-fei
    Food and Fermentation Industries. 2017, 43(7): 265.
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    Acrylamide is a potentially harmful chemical to human health which often formed during high-temperature Maillard reaction.However,Maillard reaction has an important role on food color and flavor.The inhibition of acrylamide in foods got many attentions in domestic and abroad.Microorganism which is widely used in the food industry was found to be able to inhibit the acrylamide formation in foods.The acrylamides in foods and the pathways of inhibiting formation of acrylamide are introduced.The paper is mainly focused on the inhibition formation of acrylamide in food processing by microorganism pretreatment and hope the study can provide a reference for further study on reducing acrylamides in foods.
  • ZHANG Chen-xi,HE Zhi-fei,LI Hong-jun
    Food and Fermentation Industries. 2017, 43(7): 271.
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    Bacteriocins from lactic acid bacteria are natural biological preservatives,which can inhibit or kill pathogenic microorganisms and spoilage microorganisms in foodstuffs and ensure food safety.The classification,action mechanism,separation and purification of bacteriocins from lactic acid bacteria and their applications in the preservation of meat products were summarized in this paper.The research directions in future were also prospected.