LI Na, WANG Xin-zhi, WU Hao, YI Rui-zao, CHENG Jian-ming, LIU Rui, WANG Ling-chong, XIE Dong-yun, CHEN Tao
The qualitative analysis of free fatty acids in four shellfish flesh in the coast of Jiangsu was performed using GC / MS method. Moreover,the HPLC method to determine cis-5,8,11,14,17-eicosapentaenoic acid( EPA)and cis-4,7,10,13,16,19-docosahexaenoic acid( DHA) in these shellfish flesh was first established; the dynamic accumulation law of polyunsaturated fatty acid EPA and DHA in four shellfish flesh was tested to find out the optimal harvest time. The Waters Sun Fire C18column( 2. 1 mm × 100 mm,3. 5 μm) was used at a flow rate of 0. 6 m L / min with acetonitrile( containing 0. 1% phosphoric acid)-water( containing 0. 1% phosphoric acid)( 76 ∶ 24) as the mobile phase. The eluent was detected at 205 nm. By the above-mentioned method,EPA-methyl ester and DHAmethyl ester exhibited good linear relationship within the range of 1. 005 0 ~ 80. 40 μg / m L and the range of 1. 106 2~ 88. 48 μg / m L respectively,with high sensitivity and good reproducibility. The total contents of Eicosapentaenoic acid and Docosahexaenoic acid in four shellfish flesh were significantly different in different harvest time,where June or July turned out to be the optimal harvest time. The total contents of EPA and DHA in Ruditapesphilippinarum ranged from 771. 56 μg / g to 4 565. 38 μg / g and,Meretrixmeretrix linnaeus,Cyclinasinensis and Mactraveneriformis reeve was 263. 11 μg / g to 3 115. 78 μg / g,5 907. 83 μg / g to 12 023. 06 μg / g and 422. 71 μg / g to 2 416. 27 μg / g,respectively. Based on the production and total contents of effective constituents( EPA and DHA),the best harvest time for Ruditapesphilippinarum,Cyclinasinensis,Mactraveneriformis reeveisin June,and Meretrixmeretrix linnaeusis in July.
