25 September 2019, Volume 45 Issue 18
    

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  • SUN Jinyuan, JIANG Yunsong, LIU Guoying, LI Hehe, WU Jihong, SUN Xiaotao, SUN Baoguo
    Food and Fermentation Industries. 2019, 45(18): 1-8. https://doi.org/10.13995/j.cnki.11-1802/ts.021032
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    In order to trace the source of oligopeptides in Baijiu, Jiupei from Gujinggong was used to isolate oligopeptides and their structures were identified by HPLC-quadrupole-time of flight-MS/MS spectrum (HPLC-Q-TOF-MS/MS). The antioxidant activity and angiotensin-converting enzyme (ACE) inhibitory activity of the oligopeptides were determined in vitro. Two polypeptides, Lys-Gly-Pro (KGP) and Val-Pro-Asp (VPD), were firstly identified and showed strong ABTS radical scavenging ability (1.12 μmol TE/μmol KGP and 1.36 μmol TE/μmol VPD) and oxidative radical absorption capacity (ORAC) (1.11 μmol TE/μmol KGP and 1.17 μmol TE/μmol VPD) compared with standard antioxidant Trolox. KGP and VPD also possessed ACE inhibitory activity with IC50 of 2.91 mmol/L and 2.11 mmol/L, respectively. This study provides a reference for both exploration of bioactive components in Jiupei and improvement of the health function of Baijiu by ameliorating distillation process.
  • XIE Ying, LI Xiaomin, CAI Guolin, LU Jian
    Food and Fermentation Industries. 2019, 45(18): 9-14. https://doi.org/10.13995/j.cnki.11-1802/ts.021293
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    The induction condition and binding activity of N-terminal of Lg-Flo1 protein with mannose were studied. The recombinant expression vector pETFL1 with N-terminal sequence of Lg-FLO1 gene from Pasteurella pastoris was digested, identified and transformed into Escherichia. coli BL21 (DE3). The positive transformants were identified and Lg-FLO1 gene was expressed and the effects of induction condition on expressing target protein were studied, followed by determining the activity of target protein. The expression level of N-lg-Flo1 protein in LB medium was higher than that in TB medium. Compared with IPTG induction, the target protein was expressed at a higher level with lactose. The highest expression level of the target protein was obtained after 6 h induction with 0.2 g/L lactose at 37℃. Besides, the fluorescence spectrum analysis revealed mannose-binding activity of the renatured N-Lg-Flo1 protein. This research establishes a base for large-scale production of flocculant proteins that can be used as raw materials to prepare specific adsorbents for carbohydrates.
  • HUANG Zhengchao,SONG Lianjun,HUANG Xianqing,QIAO Mingwu, ZHAO Qiuyan,ZHANG Pingan,LIU Xi
    Food and Fermentation Industries. 2019, 45(18): 15-21. https://doi.org/10.13995/j.cnki.11-1802/ts.020776
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    In order to improve the utility of microbial resources for fermented sausages and to evaluate their safety, high-throughput sequencing and subsequent bacterial diversity analysis were conducted for detecting the bacterial community in different traditional fermented sausages collected from Chengdu (SC), Shenzhen (GD), Changsha (HN) and Harbin (HB). The results indicated that Firmicutes, Cyanobacteria and Proteobacteria were dominant bacteria in all four regions. Larger bacterial communities were revealed in sausages from SC and HB. Lactobacillus sp. and Staphylococcus sp. were dominant bacteria in SC sausages while Lactococcus sp., Acinetobacter sp. and Macrococcus sp. were dominant in GD sausages, Lactococcus sp., Tetragenoccus sp. and Lactobacillus sp. were dominant in HN sausages with unidentified bacterial genera being dominant in HB sausages. In all sausages except those from HB, lactic acid bacteria were dominant and the presence of Macrococcus caseolyticus promoted the decomposition of fat and protein in fermented sausages. Therefore, M. caseolyticus can be considered as a good candidate for sausage fermentation while its safety needs to be investigated in further.
  • ZHENG Wen, YANG Xingbian, KANG Jichuan, LI Hongqing
    Food and Fermentation Industries. 2019, 45(18): 22-26. https://doi.org/10.13995/j.cnki.11-1802/ts.020905
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    The antifungal activity of fermentation broth of Lysinibacillus fusiformis He14 was analyzed, and eight pathogenic fungi were selected in this study. The stabilities of the antifungal components during extraction, precipitation and macroporous resin adsorption processes were investigated, respectively. It was found that the fermentation broth of strain He14 had inhibitory effects on all the eight pathogenic fungi and the bioactive substances were stable under extreme conditions of temperature, pH and UV. Moreover, LX-60 macroporous resin showed the best capability to adsorb and desorb bioactive components and three mixed components obtained through silica gel column chromatography showed inhibitory effects against Botrytis cinerea, Phytophthora infestans, Alternaria solani, Thanatephorus cucumeris and Trichophyton mentagrophytes. Component Ⅱ exhibited the highest inhibition effect on P. infestans. It was concluded that L. fusiformis He14 has excellent traits in antipathogenic fungus, which provides an experimental basis for the application of L. fusiformis in the fields of biological control and medicine.
  • LIANG Yexing, ZHANG Ling, GAO Feihu, ZHANG Xuemei, LI Xue, ZHANG Huanhuan, YANG Shixiong, XIONG Jiayan
    Food and Fermentation Industries. 2019, 45(18): 27-34. https://doi.org/10.13995/j.cnki.11-1802/ts.021057
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    Factors that influence the unique taste of Chongqing Shuidouchi were explored in this study. Chongqing local soybean was fermented by traditional Shuidouchi fermentation process. The contents of NaCl, reducing sugar, total acid and free amino acids were determined during fermentation, followed by sensory evaluation and correlation analysis. The results showed that the taste was mainly originated from post-fermentation. The free amino acid content increased by 11.25 times at the end of post-fermentation. In which, the umami amino acids that were responsible for umami taste, accounted for 13.45%, and the sweet amino acids accounted for 22.25%. Reducing sugars and sweet amino acids together contributed to the sweet aftertaste. The salty taste came from increased NaCl content and total acid attributed the weak sour taste of Shuidouchi. Upon these factors mixed with pepper and ginger, the unique taste of Chongqing Shuidouchi was formed. These results provide references for research of condiments in Chongqing district.
  • ZHANG Wanjun, LAN Weiqing, LAI Qingyun, ZHANG Ju, QIU Weiqiang, XIE Jing
    Food and Fermentation Industries. 2019, 45(18): 35-42. https://doi.org/10.13995/j.cnki.11-1802/ts.020293
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    The effects of slurry ice (SI) and acidic electrolyzed water ice (AEWI) treatments on quality, ATP-related compounds and microorganisms in perch during ice storage were investigated. It was found that SI and AEWI treatments had positive effects on the texture properties and slowed down the increases in TVB-N value, K value as well as the growth of Pseudomonas sp. and Shewanella sp. In SI and AEWI treated samples, the content of ATP and inosine monophosphate (IMP) and Fr value were higher than that of control whereas the content of hypoxanthine (Hx) was significantly lower (P<0.05). Furthermore, there was a significant negative interaction between texture profile and microorganisms. Similar phenomenon was also observed between microorganism and IMP. While positive interactions were found between microorganisms and hypoxanthine nucleotide, as well as between microorganisms and Hx indexes. The interactions between microorganisms and variables could represent the changes in freshness of perch during ice storage. In conclusion, SI and AEWI as a replacement for crush ice can greatly prolong the shelf life, which provides a reference for optimizing the preservation techniques of aquatic products.
  • LI Mingjie, YANG Bo, ZHAO Jianxin, ZHANG Hao, CHEN Wei
    Food and Fermentation Industries. 2019, 45(18): 43-49. https://doi.org/10.13995/j.cnki.11-1802/ts.021111
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    The aim of the current research was to establish an effective, rapid and low-cost method to identify Bifidobacterium longum subsp. infantis. The subspecies of 13 genomes sequenced B. longum strains were confirmed according to the phylogenetic tree based on orthologous genes in 13 subspecies, seven strains were B. longum subsp. longum, five strains were B. longum subsp. infantis and one was B. longum subsp. suis. A method for rapidly distinguishing B. longum subsp. infantis was confirmed by analyzing their carbohydrate utilization capacity and amplification of specific genes. The results indicated that it was impossible to distinguish subspecies based on carbohydrate utilization capacity due to strains from each subspecies were different. As comparison, the sugar kinase genes in B. longum subsp. longum and sialidase genes in B. longum subsp. infantis could be efficiently used for distinguishing B. longum subsp. infantis. With this method, five strains of B. longum subsp. infantis were identified from 40 strains of B. longum. Four of the five strains of B. longum subsp. infantis were randomly selected and further confirmed as B. longum subsp. infantis. Therefore, this study verifies that B. longum subsp. infantis can be rapidly distinguished by amplifying specific genes.
  • ZHANG Zhijun, YUE Jie, YIN Hua
    Food and Fermentation Industries. 2019, 45(18): 50-57. https://doi.org/10.13995/j.cnki.11-1802/ts.020341
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    In order to improve the identification efficiency of malt varieties and purity, thus reduce the cost of detection, SSR (simple sequence repeats) fluorescent markers were used to perform core primer screening, multiplex PCR and fingerprint data analysis on 24 malt varieties. A suitable multiple fluorescence detection system for malt identification was constructed. 12 pairs of primers were selected from the polymorphic primers screened in the previous stage for combination design. The multiplex PCR system was constructed by optimizing the primer concentration and annealing temperature, and 3 primer combinations were successfully amplified. According to the principle of fingerprint identification, HVM68, EBmag0007 and Bmac755 were identified as the core primers for cultivar differentiation, with each pair producing an average of 8.3 alleles. Using 3 pairs of core primers to construct a malt species fingerprint database, fingerprint coding could achieve rapid differentiation of all 24 malt varieties. This study established an accurate, rapid and high-throughput method for the identification and detection of malt varieties, providing technical support and quality assurance for beer companies in malt procurement and brewing formulations.
  • LI Fengzhu, ZHANG Yuli, YANG Jixia
    Food and Fermentation Industries. 2019, 45(18): 58-64. https://doi.org/10.13995/j.cnki.11-1802/ts.020771
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    In order to fast determine the whole microbial community in a high salt environment during the curing process of Zhacai (cured Tuber Mustard), a method for accurate quantification of all bacteria and fungi was developed. The 16S rDNA of Lactobacillus plantarum and the ITS of Saccharomyces cerevisiae cloned in a plasmid were used as standard references and a real-time quantitative PCR (qPCR) assay was established to detect the quantity of bacteria and fungi in brines during three curing periods. Besides, the physical and chemical indices of brine were also determined. The linear coefficients of the qPCR standard curves were greater than 0.99 and the amplification efficiencies were within 95%-105%. The pH value of the brine continuously decreased during the curing process. Whereas, the concentration of NaCl and acidity initially increased and then subsequently decreased during the second and third periods. Meanwhile, bacteria and fungi changed in the ranges of 108.10-1010.43 and 105.29-107.75 copies/μL, respectively. In addition, bacteria showed significant quantitative multiplication during all three curing periods while the count of fungi only increased during the third period. This study provides a reference for microbial control during Zhacai curing process
  • SHAO Tingting, JIANG Xue, ZHANG Min, ZHAO Yuxuan, HAO Shuang, LI Jianguo
    Food and Fermentation Industries. 2019, 45(18): 65-72. https://doi.org/10.13995/j.cnki.11-1802/ts.020923
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    This research aimed to study the effects of forced convective hot air treatment on AsA-GSH cycle and the resistance of green pepper fruit to low temperature stress. The green pepper fruits were treated with 50°C forced convection hot air for 5, 10, 20, 30 and 40 min, while those without any treatment were used as control. It was found that forced convection hot air treatment at 50℃ for 20 min showed best results on delaying the occurrence of chilling injury of the frozen green pepper fruit and maintained significantly higher chlorophyll and titratable acid content than control group (P<0.05). At the same time, the electrolyte leakage rate, malondialdehyde (MDA), H2O2 and superoxide anion (O-2) of the green pepper fruits were significantly lower than those in the control group (P<0.05). The contents of ascorbic acid (AsA) and reduced glutathione (GSH) as well as the activities of ascorbic peroxidase (APX) and glutathione reductase (GR) were also significantly lower than those in the control group (P<0.05). The results revealed that forced convection hot air treatment could effectively reduce the damage of reactive oxygen species (ROS) to cells under low temperature stress and delay the chilling injury of green pepper fruits, which provides a theoretical basis for the storage and preservation of green pepper fruits and for the response mechanism to cold stress in post-harvest vegetables.
  • ZHANG Jiangtao, ZHANG Ruixue, FANG Lei, WEI Ying, LING Kong, ZHOU Ming, GU Ruizeng, LU Jun, LI Guoming
    Food and Fermentation Industries. 2019, 45(18): 73-79. https://doi.org/10.13995/j.cnki.11-1802/ts.020908
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    To establish and analyze an oyster-sensitized rat model, female brown Norwegian rats (BN rats) were sensitized by intragastric and intraperitoneal injection using the oyster allergen Cra g 1. The allergic reaction and changes of body weight, body temperature, organ index and blood index of each group of BN rats were observed. The changes of antibody and histamine were detected by ELISA, while the changes of tissues and organs were observed by hematoxylin-eosin (HE) and toluidine blue staining. The results showed that all the experimental BN rats had allergic reaction, while the control group had no obvious symptoms. The body weight of the experimental group was significantly lower than that of control group, the organ index was significantly higher than that of control group, and the number of cells in the blood changed. In addition, the levels of antibodies and histamines in the experimental group were higher than those in the control group during the sensitization period. The visceral lesions and serious degranulation of intestinal mast cells were observed in experimental rats. In this study, Cra g 1 protein sensitized serum was obtained, and BN rats were proved to be a good animal sensitization model, which provides a good basis for studying the allergen sensitization mechanism and immune regulation in vivo.
  • ZHOU Fang, GAO Li, CHEN Jia, YE Fayin, ZHAO Guohua
    Food and Fermentation Industries. 2019, 45(18): 80-86. https://doi.org/10.13995/j.cnki.11-1802/ts.021002
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    The aim of this study was to explore the relationship between mobile phone digital images and β-carotene content in sweet potato. A smart phone was used to collect the image of sweet potato slices. Moreover, the relationship between the RGB information of the images and the content of β-carotene in sweet potato was studied, and predict models were calibrated. The results showed that the content of β-carotene in sweet potato had a strong negative correlation with green (G) value, and a moderate negative correlation with blue (B) value. There was no correlation between β-carotene content and red (R) value of sweet potato slice images. The logarithmic function was used to fit the relationship between G value and β-carotene content. The coefficient of determination (R2) of the model reached 0.947, the predicted correlation coefficients (rp), root mean square error of prediction (RMSEP) and standard deviation ratio (SDR) of the model were 0.983, 0.819 and 5.269, respectively. Overall, digital images of mobile phone can reflect the differences of β -carotene content in sweet potato, and the RGB information of digital image can accurately estimate the β-carotene content of sweet potato, which provides a new idea for rapid detection of sweet potato quality.
  • QIU Xiaodong, TAO Ningping
    Food and Fermentation Industries. 2019, 45(18): 87-92. https://doi.org/10.13995/j.cnki.11-1802/ts.020972
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    In order to study the hypoglycemic effect of konjac cake containing chia seed oil, the physicochemical properties and fatty acid composition of spiral pressed chia seed oil were analyzed by mouse feeding experiment. The results showed that the physicochemical refraction coefficient of chia seed oil was 1.4806; the iodine value was 194g /100g; the saponification value was 190.1mgKOH/g; the insoluble impurity was less than 0.01%; the acid value was 0.55 mgKOH/g; and the peroxide value was 6mmol/kg. In terms of fatty acid composition of chia seed oil, alpha-linolenic acid and linoleic acid accounted for 59.35% and 19.59%, respectively, and PUFA/SFA was 7.07. By comparing the body weight and fasting blood glucose of mice under two different feeding conditions (the group of konjac cake containing chia seed oil, the group of konjac cake without chia seed oil), no significant difference in body weight regulation was found (P>0.05). However, there was a significant difference in fasting blood glucose regulation in mice (P<0.05). The fasting blood glucose values of the two groups were 5.98 mmol/L and 6.84 mmol/L(the model group was 7.99 mmol/L)after feeding, which showed that konjak cake containing chia seed oil had better effects on blood glucose regulation in mice. It provides theoretical basis for the preparation of new products of chia seed oil and konjac powder.
  • ZHENG Jiong, LI Wei, CHEN Guangjing, KAN Jianquan
    Food and Fermentation Industries. 2019, 45(18): 93-100. https://doi.org/10.13995/j.cnki.11-1802/ts.019895
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    The changes in flavor compounds of bamboo shoots during pickling process were studied. The content of volatile components, organic acids and amino acids in the pickling process was analyzed with the bamboo shoots (Dendrocalamus latiflorus) as raw materials. The results showed that 74 kinds of volatile flavor compounds, 8 kinds of organic acids and 17 kinds of amino acids were detected in 63 days of pickling. Among them, the relative contents of phenols, ketones and alkanes increased gradually with the prolongation of pickling time, the relative contents of aldehydes, alkenes and other substances decreased, and the contents of alcohols and esters increased first and then decreased. Besides, the volatile components with high relative contents were 4-methylphenol, 2-pentylfuran, hexanal, 4-hydroxybenzaldehyde, linalool, etc. The contents of lactic acid, acetic acid and propionic acid increased after pickling, while other 5 kinds of organic acids decreased significantly. The content of most amino acids showed a rapid decreasing after pickling. The results can provide a theoretical basis for the evaluation and control of flavor quality of bamboo shoots during pickling process.
  • HU Xiaoqian,LI Changjiang,WU Yongxiang,QI Lulu, WANG Yuqin,HUANG Hui, HAN Tingting
    Food and Fermentation Industries. 2019, 45(18): 101-106. https://doi.org/10.13995/j.cnki.11-1802/ts.020699
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    Nutritional components including pH value, moisture, ash, protein, total soluble sugar, Vc content of Chengkan Luo’s hairy tofu were analyzed, and the effects of different extraction temperature and solid-liquid ratio on antioxidant activity in vitro were studied. The results showed that Luo’s hairy tofu was rich in nutrients, with protein content 20.830 mg/mL, total soluble sugar 0.784 g/100 g and Vc content 1.038 mg/100 g, which were higher than those of common hairy tofu. Statistical analysis revealed that the total reducing power, scavenging ability of superoxide anion radicals, hydroxyl radicals, DPPH·,·OH and ABTS+· obtained at different conditions were significantly different. Moreover, the content of soluble components and the antioxidant activity were the highest under the conditions of extraction temperature 100℃ and solid-liquid ratio 1∶5. This study emphasizes the possibility of using Chengkan Luo’s hairy tofu as an antioxidant functional food, and provides a theoretical reference for its industrial processing.
  • DIAO Xiaoqin, GUAN Haining, LI Yang, LIU Limei
    Food and Fermentation Industries. 2019, 45(18): 107-112. https://doi.org/10.13995/j.cnki.11-1802/ts.020838
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    To investigate the effects of high pressure homogenization on the emulsification of myofibrillar protein (MP), the emulsifying and structure properties of MP treated with different homogeneous pressures (20 MPa and 40 MPa) once and twice were examined. The results showed that homogenization treatment could improve the solubility, emulsifying activity index (EAI) and emulsion stability index (ESI) of MP, enhance the absolute value of the zeta potential, and reduce the protein particle size. Meanwhile, 40 MPa homogenization treatment once had the most significant effect. With the increase of homogeneous pressure, the total sulfhydryl content decreased, while active sulfhydryl content increased first and then decreased, and the surface hydrophobicity showed an increasing trend. The infrared spectrum showed that homogenization treatment could change the secondary structure of MP by decreasing the content of α-helix and β-sheet, while increasing the content of β-angle and random crimp. In conclusion, high pressure homogenization can improve the emulsification characteristics of MP, which provides theoretical basis for the application of high pressure homogenization in the actual production of myofibrillar protein and for improving the quality of meat products.
  • WEI Guangqiang,CHEN Yue, ZHUO Jiazhen, ZHAO Cunchao, WANG Xuefeng, HUANG Aixiang
    Food and Fermentation Industries. 2019, 45(18): 113-119. https://doi.org/10.13995/j.cnki.11-1802/ts.020820
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    The qualitative characteristics and changes in the main flavor components of yogurt were analyzed during fermentation and storage. The physicochemical indexes (e.g. pH, acidity, water holding capacity and viscosity), the number of active lactic acid bacteria and the content of flavor components (e.g. amino acids and fatty acids) were determined. The results showed that both water holding capacity and viscosity of yogurt gradually increased at early stage of fermentation and cold storage, and then slowly decreased after this stage. The acidity of yogurt increased and the pH decreased significantly (P<0.05) during the fermentation process, and there were no significant changes during cold storage. During fermentation and storage, the number of lactic acid bacteria, total amino acids, essential and non-essential amino acids, as well as contents of umami amino acids gradually increased and subsequently decreased and then slowly increased. Furthermore, the content of fatty acids continually increased during fermentation and cold storage, polyunsaturated fatty acids gradually decreased, while short-, medium-and long-chain fatty acids gradually increased but all with no significant changes. This study provides a theoretical basis for developing and evaluating the quality of yogurt and other fermented dairy products.
  • LI Mingqi, HE Zhifei, LI Shaobo, LI Ranran, LI Hongjun
    Food and Fermentation Industries. 2019, 45(18): 120-129. https://doi.org/10.13995/j.cnki.11-1802/ts.021040
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    In order to improve the tenderness and water holding capability of rabbit meat, the hind leg meat from Yila rabbit was treated with a combination of CaCl2, ficin and kiwifruit protease as tenderizers. The optimal ratio of tenderizers and the optimal tenderizing condition of compound tenderizer were determined according to the analysis of the shear force, cooking loss, myofibrillar fragmentation index and texture profile of the meat. The results showed that the compound tenderizer had the best tenderizing effect with the optimal proportion (18 mg/L CaCl2, 11 mg/L ficin and 6 mg/L kiwifruit protease) under the optimized condition (tenderized at pH=8 and 30 ℃ for 90 min). Under this condition, the shear force and the cooking loss of the rabbit hind leg meat were 15.49 N and 25.73%, respectively, which reduced by 50% and 17%, respectively, compared to the control. In addition, the hardness of tenderized meat decreased by 65%, the myofibrillar fragmentation index and the elasticity increased by 54% and 40%, respectively. The results showed that the compound tenderizer can significantly improve the tenderness and water holding capability of rabbit meat, which has certain reference value for rabbit meat processing.
  • YAN Ran, YANG Xiaoying, FU Maorun, DU Yamin, SUN Fei, LIU Yamin, WANG Jinling, DOU Guanglei
    Food and Fermentation Industries. 2019, 45(18): 130-136. https://doi.org/10.13995/j.cnki.11-1802/ts.020902
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    This study was conducted to extend the shelf life of raspberry (Rubus idaeus L.) during storage and transportation. After treatment with CaCl2 or CaCl2 combined with exogenous pectin methylesterase (CaCl2-ePME), the Vc content, total acids and organic acids of raspberry were determined during 4 oC storage. Compared with two treatment methods, CaCl2-ePME was more effective for the stability of tartaric acid, malic acid, citric acid and succinic acid in raspberry. After 12 days of storage, the total acids of control group decreased by 37% contrary to 17% (P<0.05) in experimental group and the Vc content of experimental group was 1.67 times more than control. In conclusion, the combined treatment of CaCl2 and ePME could help maintain the quality and improve the flavor of raspberry, which provides a new preservation technology for raspberry and some other berry fruits.
  • XIANG Jinle, ZHAO Shengjuan, MA Liping, JIN Wengang, FEI Peng,FAN Jinling, ZHU Wenxue
    Food and Fermentation Industries. 2019, 45(18): 137-143. https://doi.org/10.13995/j.cnki.11-1802/ts.021093
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    Feasibility of fresh edible peony flower as raw material was explored in liquid functional food production. Juice from fresh ‘Fengdan Bai’ peony flower was obtained by enzymatic treatment followed by phytochemical profile investigated by high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry. The results showed that the yield of peony flower juice was improved by 22.3% through treatment with a combination of pectinase, cellulase and papain (in a ratio of 1∶2∶1). The optimized enzyme treatment condition was at 50 ℃ for 90 min with 0.15% enzyme. There were 20 components identified in peony flower juice, including three organic acids, eight phenolic acids, seven flavonoids and two monoglycosides. Phenolic acids and flavonoid glycosides were the main active components and were mainly in the forms of gallic acid, galloyl glucose, gallotannins, apigenin glucoside, apigenin neohesperidoside and kaempferol glucoside. The results provide a basis for processing technology of peony flower juice as well as its application in liquid functional foods.
  • YANG Jie, WANG Yuhua, MI Lan, LI Aixia, WANG Jing
    Food and Fermentation Industries. 2019, 45(18): 144-154. https://doi.org/10.13995/j.cnki.11-1802/ts.020961
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    The effects of Laschancea thermotolerans on enhancing the acidity and aroma of Chardonnay dry white wine were studied. Sequential inoculation of one of the three different strains of L. thermotolerans with Saccharomyces cerevisiae at 4 d interval was conducted. Yeast growth was monitored during fermentation and the main physicochemical indexes, aroma components and sensory quality of the wine were analyzed. The results showed that alcohol fermentation completed on the 11th day in experimental groups and the population of all three strains of L. thermotolerans reached 107 CFU/mL on the 4th day and died completely on the 8th to 9th day. The pH value and the contents of volatile acids of the experimental groups were significantly lower than that of the control while the amount of L-lactic acid produced significantly increased by 1.41-2.42 g/L. The composition and contents of aroma components were significantly different among experimental groups, and the contents of aldehydes, ketones, terpenoids and fatty acid ethyl esters (e.g. ethyl octanoate, ethyl decanoate and ethyl laurate) increased significantly compared against the control. Sensory analysis showed that the treated wine samples had higher acidity, rich fruity flavor and complex aroma. The results provide data support and theoretical reference for acid enhancement technology and quality improvement of dry white wine.
  • ZHANG Ji, MENG Guodong, PENG Guilan, ZHANG Xuefeng, YANG Ling
    Food and Fermentation Industries. 2019, 45(18): 155-161. https://doi.org/10.13995/j.cnki.11-1802/ts.020875
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    The study was conducted to improve the mechanical drying efficiency as well as the quality of dried rice, and reduce the energy consumption of drying. Taking average drying rate (r), crackle ratio (b) and unit energy consumption (e) as indicators, hot air temperature (X1), hot air speed (X2), moisture content at conversion point (X3) and vacuum temperature(X4) as test factors, a Box-Behnken Design (BBD) was designed to optimize the parameters of hot air and vacuum combined drying process for rice. The results were compared with the corresponding index values of single drying methods. The results showed that the optimum parameters of combined drying were: X1=40 ℃, X2=0.7 m/s, X3=20.7 %, X4=38.1 ℃. In addition, the average drying rate was 0.000 483 g/(g·min), the crackle ratio was 6.3%, and the unit energy consumption was 2 612 kJ/kg. The average drying rate of combined drying was 29.5% lower than hot air drying and 33.1% higher than vacuum drying. The crackle ratio was 10% lower than hot air drying and 13.7% lower than vacuum drying. The unit energy consumption was reduced by 60.1% compared to hot air drying and 12.6% to that of vacuum drying. Overall, hot air and vacuum combined drying method for rice has obvious advantages over single drying methods, which provides a theoretical basis for its practical application.
  • MA Fei, HUAN Yanjun, DIAO Xinyue, LIU Li
    Food and Fermentation Industries. 2019, 45(18): 162-167. https://doi.org/10.13995/j.cnki.11-1802/ts.020357
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    In order to shorten the seasoning time of the soy sauce flavored seasoned meat products, and improve the texture of the product without affecting the flavor characteristics of the product, the preparation process of sauced meat flavor liquid based on the injection-rolling process was optimized. The seasoned brine was prepared from halogen concentrate and Maillard reaction liquid in a certain proportion. Based on the sensory score, the concentration degree of halogen concentrate, the reaction time of Maillard reaction, and the mixing ratio were optimized. The results showed that the optimal parameters were 15% of concentration degree, 10 min of reaction time, and 9∶2 of the halogen concentrate than the Maillard reaction solution. Under these conditions, the sensory score of the sauced meat products reached 87.23, with 1.34% relative error compared with theoretical prediction. In addition, the texture score and the comprehensive sensory score of products using injection-rolling technique were improved, and there was no significant difference in flavor scores compared to the traditional sauced products.
  • QIAN Lei, ZHANG Yeni, LI Tao, ZHANG Zhijun
    Food and Fermentation Industries. 2019, 45(18): 168-175. https://doi.org/10.13995/j.cnki.11-1802/ts.021018
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    The fresh-keeping effects of carboxymethyl chitosan and Pholiota nameko peptides on Pleurotus eryngii was studied. P. eryngii was stored at 4 °C for 30 days after coated with carboxymethyl chitosan and P. nameko peptides. The changes in sensory quality, oxidative stress level, antioxidant enzymes activity and soluble solid content were determined. The results indicated that after 30d of storage, each coating treatment group could maintain color, hardness, superoxide dismutase and catalase activity, as well as reducing weight loss and respiratory intensity. Meanwhile, it inhibited the increase of malondialdehyde content and polyphenol oxidase activity, yet slow down the decrease of Vc and soluble sugar content, which was significantly different from the control group (P<0.05). Compared with carboxymethyl chitosan, the weight loss rate and respiratory intensity of complex coating reduced to 55.24% and 77.35% after 30d of storage, and the content of malondialdehyde and phenol oxidase activity decreased to 82.61% and 81.11%. In addition, superoxide dismutase and catalase activities increased by 20.83% and 44.97%, respectively, while Vc and soluble sugar content increased by 31.05% and 29.86%, respectively. Therefore, carboxymethyl chitosan and P. nameko peptides coating can inhibit the deterioration of sensory quality of Pleurotus eryngii during low temperature storage, thus delaying the aging process and reducing nutritional losses, which provides theoretical basis for the development of complex biological preservative and extending its shelf life.
  • WANG Ling, TIAN Bing, PENG Lin, KAN Jianquan, CHEN Hourong
    Food and Fermentation Industries. 2019, 45(18): 176-182. https://doi.org/10.13995/j.cnki.11-1802/ts.020536
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    The effects of hot air-microwave drying process on the quality and energy consumption of Zanthoxylum schinifolium were studied. Through single factor tests with chromatic aberration as the observation index, the suitable range of hot air temperature, converted moisture content and microwave power was determined. Subsequently, Box-Behnken central combination test was carried out, and response surface analysis was conducted with the comprehensive score of chromatic aberration, volatile oil content and unit energy consumption as the response values. Results showed that the optimum condition for hot air-microwave combined drying of Zanthoxylum schinifolium was: hot air temperature 64.56 ℃, conversion moisture content 41.59 %, and microwave power 345.20 W. Under this condition, the composite score of dry Zanthoxylum schinifolium was 0.194 522. The error between the test result and optimization result was less than 4%, indicating that the optimization result was reliable. Hot air-microwave combined drying of Zanthoxylum schinifolium is of great significance for improving quality and reducing energy consumption, which provides a theoretical basis for the industrial application.
  • LI Binbin,ZHAO Xiaomin,ZHOU He,WU Min,ZHANG Yafei,YAO Yao, QU Yiming,LI Xuewen
    Food and Fermentation Industries. 2019, 45(18): 183-188. https://doi.org/10.13995/j.cnki.11-1802/ts.021295
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    The brewing test of Merlot was carried out by different cold maceration (CM) time treatments (3, 5, 7 d) before fermentation, and the effects of CM time on phenolic substances and color of wine, as well as their correlations were studied. The results indicated that CM caused pH reduction and total acid content enhancement of wine. The content of phenols in dry red wine significantly increased after CM treatment (P<0.05). The contents of total phenols, total tannins, total anthocyanins and total flavonoids increased as elongation of CM time, while the content of total flavanols was not affected. Moreover, different CM time caused the increase in the red color hue (P<0.05) and the enhancement of color saturation. Principal component analysis showed that prolonged CM time was beneficial to extract and polymerize anthocyanins and other phenolic substances, consequently improved the color stability of wine. In summary, 7 d was an ideal period of CM treatment to produce high quality dry red wine as both content of phenolic substances and color improved.
  • XING Shijun, SHI Ling, LIU Guangjuan, ZHOU Ye, GONG Caixia, WANG Zirong
    Food and Fermentation Industries. 2019, 45(18): 189-194. https://doi.org/10.13995/j.cnki.11-1802/ts.021311
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    In order to select suitable fungicides for wine grapes and ensure the quality and safety of wine, 5 mg/L thiophanate methyl, 2 mg/L metalaxyl and 5 mg/L azoxystrobin were added to Cabernet Sauvignon grape mash for brewing, respectively. The physical and chemical indexes and sensory quality of grape wines were compared. Whereas the residual pesticides were determined at different stages of production. The results showed that all three pesticides had negative effects on the quality of wine at various degrees. The azoxystrobin had a greater impact on the quality of wine and prolonged the fermentation time significantly, while thiophanate methyl significantly decreased the aroma quality, and metalaxyl had less impact. The three pesticides decreased by around 70% at the end of brewing. It was found that the amounts of pesticides removed at different stages of brewing were in the following order: alcohol fermentation > lactic acid fermentation > aging. Besides, pesticides with weak solid relative polarity had significant adsorption capacity. In conclusion, the fungicides affected the quality of wine, and the less polar pesticides the easier to be removed, which provides some references for fungicides application in wine grapes.
  • LI Zhen, XIAO Yang, ZHEN Tianyuan, PIAO Meizi
    Food and Fermentation Industries. 2019, 45(18): 195-201. https://doi.org/10.13995/j.cnki.11-1802/ts.020979
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    A protease-producing bacterium from sea cucumber intestinal tract was isolated and identified. Through morphological, physiological, biochemical characterization and 16S rDNA sequence homology analysis, the isolated strain was identified as Thalassobacillus sp. QDHF-1. Sea cucumber intestine waste was fermented by strain QDHF-1, and the fermentation conditions were optimized and the antioxidant activities of produced peptides were determined. The optimal fermentation condition to produce peptides was 6% inoculum, 20% raw materials and 36 h fermentation. The polypeptides could scavenge ·OH, DPPH·, and O-2·, and the highest scavenging rate of 93.94% was obtained on ·OH using 50 g/L polypeptides. The results indicate the potential applications of strain QDHF-1 in sea cucumber offal fermentation to produce bioactive peptides.
  • YANG Sen, TU Zongcai, WANG Hui, HU Yueming
    Food and Fermentation Industries. 2019, 45(18): 202-208. https://doi.org/10.13995/j.cnki.11-1802/ts.021168
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    The fresh grass carp was used as raw material to prepare superfine fish bone paste by ultrafine comminution. A new type of canned luncheon fish meat with high calcium was developed. Based on single factor experiment, according to Box-Behnken central design principle, the technological formula was optimized by sensory evaluation combined with texture analysis. The optimum additions of starch, soybean protein isolate, compound phosphate and glutamine transaminase (Tg) were 8.0%, 2.0%, 0.25% and 0.6%, respectively. Furthermore, the nutrient testing results showed that the content of protein, fat and calcium were 15.7g/100g, 3.0 g/100 g and 336 mg/100 g, respectively, meeting high calcium standard. Canned luncheon fish meat is considered a high-calcium product, suitable for all age groups. It provides a new idea for the utilization of freshwater fish processing by-products and a technical reference for the development of novel high-calcium luncheon fish.
  • CEN Jianwei,YU Futian,YANG Xianqing,LI Laihao, HUANG Hui,WEI Ya, ZHAO Yongqiang,LIN Zhi
    Food and Fermentation Industries. 2019, 45(18): 209-214. https://doi.org/10.13995/j.cnki.11-1802/ts.021022
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    In order to explore the application prospects of slightly acidic electrolytic water in the processing of tilapia, the fresh-keeping effects on tilapia fillets was studied. The tilapia fillets were soaked in slightly acidic electrolytic water with an available chlorine concentration of (31.39 ±1.48)mg/L for 10 min, followed by drained into a polyethylene bag and stored at 4 ℃. Sensory evaluation, total volatile base nitrogen (TVB-N) value, k value, total colony count and pH value were determined. The results show that during storage, the TVB-N value of the control group exceeded 20 mg/100 g on the 2nd or 3rd day, while that of the experimental group was just over 20 mg/100 g on the 6th day. In addition, the k value of the control group was already more than 60% on the 2nd or 3rd day, yet that of the experimental group reached 60% on the 5th or 6th day. The total number of colonies of the control group was more than 6 lg CFU/g on the 2nd or 3rd day, while that of the experimental group was on the 6th day. Moreover, the increase of pH in the experimental group was slower than that in the control group, and the sensory score of the experimental group was always higher than that of the control group. Therefore, the shelf life of tilapia fillets treated with slightly acidic electrolytic water was extended by 2 to 3 days compared with the control group, which provides a technical reference and basis for the application of slightly acidic electrolytic water in fresh-keeping of aquatic products.
  • GAO Yuanyuan,SHA Xiaomei,ZHANG Luzheng,GUO Debin
    Food and Fermentation Industries. 2019, 45(18): 215-221. https://doi.org/10.13995/j.cnki.11-1802/ts.020976
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    To break through the limitation of traditional cream that must be refrigerated,whipping cream powder was prepared by microencapsulation technology with spray drying. The cocoa butter substitute, dehydrated butter, and food emulsifiers were used as the core materials. Moreover, glucose syrup, sodium caseinate, dipotassium phosphate, and colloid were chosen as the wall materials. Results showed that the optimum prescription was 35% cocoa butter substitute (mass fraction of total solid, the same below), 5% dehydrated butter, 2% distilled molecular glyceride, 5% lactic and fatty acid esters of glycerol, 0.6% propylene glycol esters of fatty acid, 5% sodium caseinate, 1% dipotassium phosphate, 0.2% agar, and 46.2% glucose syrup. Whipping cream with expansion rate of 3.3 could be produced by whipping the powder for 3 minutes with double water. Furthermore, manual ice cream was prepared with 24% whipping cream powder, 30% whole milk powder, 45%white sugar, and 1%stabilizer blends. Compared with the commercial products, it was qualified in physicochemical and sensory indexes. The study of formulation and application of whipping cream powder greatly improves the convenience of whipping cream and expands its application.
  • HAN Jianxun,SUN Ruixue,CHEN Ying,SUN Chongde,WEN Zhigang
    Food and Fermentation Industries. 2019, 45(18): 222-227. https://doi.org/10.13995/j.cnki.11-1802/ts.020347
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    The aim of our research was not only to qualitatively identify camellia oil, soya bean oil, rapeseed oil and corn oil, but also to quantitatively detect the soya bean oil in adulterated camellia oil. In this study, a FTIR method was investigated for discriminating camellia oil, soya bean oil, corn oil and rapeseed oil by comparison of two characteristic peaks height (1 122 cm-1, 1 096 cm-1) among those four edible oils. And it could identify pure camellia oil and camellia oil adulterated with soya bean oil(≥30%, w/w). In conjunction with PCA algorithm, a discrimination model was also developed for qualitative detecting camellia oils and other edible oils based on analyzing the FTIR fingerprint spectra in the range of 1 464~722 cm-1. After that, for quantitative determination of soya bean oil in adulterated camellia oil, a PLSR model with the detection limit of 1% (w/w) was provided. And it obtained RMSECV value of 0.032 0 for calibration sets and RMSEP value of 0.029 7 for validation sets. R2 of both sets could reach 0.99. In conclusion, with the characteristic of convenience, sensitivity, and accuracy, the FTIR spectroscopic methods established above has the capability for rapid verification of camellia oil adulteration in the edible oil market.
  • LIU Yiqi, HU Changying, SHANG Guiqin, ZHANG Hong, ZENG Ying, ZHU Lei, ZHONG Huaining
    Food and Fermentation Industries. 2019, 45(18): 228-233. https://doi.org/10.13995/j.cnki.11-1802/ts.021013
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    A determining method for octamethylcyclotetrasiloxane (D4), decamethylcyclopentasiloxane (D5) and dodecamethylcyclohexasiloxane (D6) in cake trans-migrated from silicone baking mold was established and their safety was evaluated. Under the ultrasonic time of 20 min and repeat extraction for 3 times with acetonitrile, the gas chromatography-mass spectrometry (GC-MS) was performed to detect the contents of D4-D6 in cake baked by silicon rubber mold, and threshold of toxicological concern (TTC) was used to evaluate the safety of D4-D6. The linear range of D4-D6 was 0.12-3.00 μg/mL with the correlation coefficients (R2) greater than 0.996 9. The average recoveries of D4-D6 were 86.10%-104.62% and the relative standard deviation (RSD) were 3.16%-7.53% (n=6). The detection limits were all less than 6.86 ng/mL and quantitative limits less than 23.20 ng/mL. In addition, the total contents of D4-D6 in the cake baked by silicone rubber mold was 8.56 mg/kg. Total estimated daily intake (EDI) of D4-D6, classified as Cramer Ⅲ, was below the TTC threshold (90 μg/d). The method can simply detect the contents of D4-D6 in cake, and the safety issue of D4-D6 in the silicone rubber migrated into the food during processing cannot be ignored.
  • ZHU Cheng,TONG Guoqiang,YI Xiang,LIU Mingzhi, WAN Zhen,LUO Gaojian,YANG Qiang
    Food and Fermentation Industries. 2019, 45(18): 234-238. https://doi.org/10.13995/j.cnki.11-1802/ts.020501
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    In order to evaluate the sensory characteristics of Maopu Tartary Buckwheat Liquor quantitatively and achieve a better control of the product quality, 36 samples (with different grades, styles and production date) were evaluated according to the flavor wheel combined with existing researches on Chinese liquor flavor. The terms that were applicable to describe sensory characteristics of Maopu Tartary Buckwheat Liquor were screened, including 16 aroma description terms, four taste description terms and six mouthfeel description terms. From the aspects of color, brightness, aroma, taste, mouthfeel and style, the flavor wheel of Maopu Tartary Buckwheat Liquor was drawn. Through the flavor profile, the flavor differences among liquor samples of three different grades and styles were clearly and intuitively expressed. The results indicated that the sensory feature descriptors screened based on the flavor wheel could accurately represent the sensory characteristics of the liquor. Overall, flavor wheel can make the sensory evaluation of Maopu Tartary Buckwheat Liquor more direct and quantitative and can consequently guide better quality control.
  • CHENG Yuehong,REN Xuemei,WANG Jian, YU Yanyan,FENG Wei,LI Yuting,BAO Lianyan
    Food and Fermentation Industries. 2019, 45(18): 239-242. https://doi.org/10.13995/j.cnki.11-1802/ts.019781
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    The aim of this study was to develop an efficient method for determination of carbohydrates in soy sauce, which defined as the sum of total sugar and starch. In this study, carbohydrates content from different grades of fermented soy sauce were assayed. The accuracy and recovery rate of the method for total sugar were investigated. The established assay method for total sugar shown high accuracy, with the recovery rate of 91% to 96%. The results indicated the estimation of carbohydrate content in soy sauce through detecting total sugar and starch content had fulfilled the criteria of national standard.
  • GU Lijun, LIN Zhenhua, WU Shiyu, ZHENG Yanjie, ZHOU Xiaowen, YUAN Fuding, JIANG Peichun, LIN Changhong
    Food and Fermentation Industries. 2019, 45(18): 243-247. https://doi.org/10.13995/j.cnki.11-1802/ts.019999
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    Near-infrared spectroscopy combined with chemometrics has been used to establish identification methods for different brands of vinegar. The data of 103 groups of vinegar samples were obtained by near-infrared spectrometer, and the second derivative, standard treatment, T-text test and principal component analysis (PCA) were used to process the data, and the last linear discriminant analysis (LDA) model was constructed by using the method of one remaining method. The results indicated that compared with the original infrared spectrum, the processed data showed the clustering trend of the main components of the same brand of vinegar. The results of cross-validation demonstrated that the accuracy rate of identifying different brands of vinegar was 85.57% using PCA-LDA model, which was feasible and applicable. Overall, the method of combining near-infrared spectroscopy with PCA-LDA model has provided a rapid identification technique for different brands of vinegar, which has the potential to process near-infrared spectroscopy data to study the main components of different matter.
  • WU Zhen, LI Hong, CHEN Gang, YANG Yong, WANG Min, TIAN Yuan, LIU Yuan
    Food and Fermentation Industries. 2019, 45(18): 248-253. https://doi.org/10.13995/j.cnki.11-1802/ts.020276
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    This study investigated the application of partial least-squares discriminant analysis (PLS-DA) and clustering analysis (CA) techniques associated with inorganic element concentrations for evaluation of different varieties of pomelo (Citrus grandis L. Osbeck). The contents of 17 inorganic elements of 67 pomelo samples from 5 different varieties (Shatianyu, Yuhuanyou, Cuixiangtianyou, Guanximiyou and Liangpingyou) were determined by inductively coupled plasma-atomic emission spectrometry (ICP-AES). The results showed that Cd, Pb, As and Hg were not detected in all pomelo pulp samples and pomelo pulp was rich in Ca, K, Fe, Mg and Mn. PLS-DA could distinguish 5 different varieties and its cumulative variance contribution rate of three-factor (F1-F3) was 99.065%, revealing that F2 was related to B and V, F3 related to Mg and Mn, and F1 related to all others. 17 elements were clustered to 4 classes by correlation analysis and CA, indicating internal relations of inorganic elements in pomelo fruits. The elements coupling chemometrics could be successfully used to identify and distinguish these 5 kinds of pomelo in China. This study provides a basis for the comparison of variety characteristics, germplasm resources investigation and comprehensive utilization of pomelo.
  • WANG Xin, LIU Hebing, TAO Xiaoqi
    Food and Fermentation Industries. 2019, 45(18): 254-262. https://doi.org/10.13995/j.cnki.11-1802/ts.020946
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    As one of the broad spectrum antibacterial antibiotics, the rapid detection of chloramphenicol(CAP) is crucial because it was banned from food animals. Aptamer is a novel biological recognized element with good stability and facile synthesis. Developing sensitive, accurate and rapid detection of CAP with aptamers becomes a new breakthrough and has good development prospects compared with traditional antibody detection. Therefore, the main methods in CAP residues detection with aptamers have been reviewed in recent five years, including colorimetric, fluorescence, chemiluminescence, surface-enhanced Raman scattering and electrochemical biosensor. However, lack of systematic in-depth research, related detection applications are difficult to take into account both rapid and accurate detection requirements. It is hereby to compare different analytical methods and, moreover, analyze the advantages, disadvantages and development trends, which provides a useful reference for the efficient regulation of CAP residues in animal-derived foods.
  • JI Yaru, HU Wenzhong, LIAO Jia, JIANG Aili, XIU Zhilong
    Food and Fermentation Industries. 2019, 45(18): 263-269. https://doi.org/10.13995/j.cnki.11-1802/ts.019816
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    Blueberry, one of the berries favored by consumers, has unique flavor, and is rich in antioxidants such as anthocyanins and Vc, contributing extremely high nutritional and health care value. However, fresh blueberry, with thin peel and high water content, is highly susceptible to microbial infection at the stalk, which can cause blueberry decay, greatly shorten the shelf life of blueberry, and restrict the healthy development of blueberry industry. Therefore, it’s a hot issue in the postharvest research of blueberry to explore an efficient and safe fresh-keeping technology. The research status of postharvest physiology and pathology of blueberry and its preservation technology at home and abroad were reviewed. It was found that the senescence mechanism, microbial infection mechanism and regulation mechanism of preservation technology were still unclear. Consequently, in the future, the mechanism of blueberry postharvest senescence should be further studied at both cellular and molecular level, which can provide a theoretical basis for the research on prevention and control of blueberry postharvest diseases and preservation technology.
  • FAN Xinguang, LIANG Changchang, GUO Fengjun, JIANG Weibo, YANG Yanqing, LIU Hui, ZHANG Aidi, GONG Hansheng
    Food and Fermentation Industries. 2019, 45(18): 270-276. https://doi.org/10.13995/j.cnki.11-1802/ts.019610
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    Fruits and vegetables are prone to deterioration after postharvest. Since temperature is a key factor affecting quality, near freezing temperature (NFT) storage has become a research hotspot, and been widely used in the field of fruit and vegetable preservation. It is a precise temperature-controlled and fresh-keeping technology by storing foods near biological freezing points, which can effectively delay the postharvest senescence process, and significantly extend shelf-life of fruits and vegetables. This review summarized the researches on NFT storage, and analyzed the postharvest biological metabolic process of fruits and vegetables, including ethylene production and respiration ration, loss of nutrition content, texture and softening process, antioxidant capacity and membrane lipid peroxidation, sensory quality and microbial growth. This study can be useful for the application of NFT storage of fruits and vegetables.
  • JI Xiaojiao, YAN Wenjie, ZHANG Jingjie, HAN Di, REN Guangxu, WANG Jing
    Food and Fermentation Industries. 2019, 45(18): 277-283. https://doi.org/10.13995/j.cnki.11-1802/ts.021242
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    Silkworm pupa protein is a high-quality natural nutritious animal protein with a broad market prospect, due to its properties of anti-tumor, anti-oxidation, blood pressure-lowering and anti-fatigue. The nutrient index, extraction, refining, and functional properties of silkworm pupa protein, as well as its applications in foods were summarized. This review provides some references for further researches and development of silkworm pupa protein.
  • JIANG Bao
    Food and Fermentation Industries. 2019, 45(18): 284-292. https://doi.org/10.13995/j.cnki.11-1802/ts.021291
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    Fruit heterogeneity is an important factor that affects grape composition and wine quality. Research methods of fruit heterogeneity as well as its effects on wine grapes and wines were reviewed. Four main classification methods for fruit heterogeneity were summarized from the aspects of fruit weight, diameter, berry density and soluble solids content with harvest time. The latest research regarding the effects of fruit heterogeneity on physicochemical composition, nutritional characteristics and aroma compounds of wine grapes and corresponding wines were illustrated. Besides, the existing problems were analyzed and future research directions were emphasized. It was found that fruit heterogeneity has effects on the physicochemical composition of wines, such as reducing the content of malic acid and pH value, deepening the color of wines and changing the content of titratable acids. It also increases the content of phenolic substances in small fruits, changes some individual phenolics concentration in berries and increases the antioxidant capacity of high-density berries. Additionally, the content of some aroma substances in wines as well as the overall aroma vary with fruit heterogeneity. Therefore, grape fruit heterogeneity can significantly affect wine style, which is worth for further research.