15 June 2020, Volume 46 Issue 11
    

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  • MA Zhenfeng, XU Meijuan, YANG Taowei, ZHANG Xian, SHAO Minglong, NAKANISHI Hideki, RAO Zhiming
    Food and Fermentation Industries. 2020, 46(11): 1-8. https://doi.org/10.13995/j.cnki.11-1802/ts.023680
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    2-Pyrrolidone as a highly promising bio-based platform chemical, has received more attention due to their widely used in textile and pharmaceutical industries. 2-Pyrrolidone synthesis pathway was established in Corynebacterium glutamicum by exploring the function of the key enzyme, CoA transferase, thus providing guidance for sustainable industrial synthesis of 2-pyrrolidone. Firstly, argB gene was knocked out to block the L-arginine biosynthesis pathway and increase the carbon flux to the 2-pyrrolidone synthesis pathway, resulting in a highly efficient synthesis of L-glutamate in the chassis cells. Secondly, a glutamate decarboxylase (Gad) was expressed to convert L-glutamate to GABA. Finally, CoA transferase derived from Anaerotignum propionicum was optimized for the N-terminus RBS, then recombined and expressed in C. glutamicum to convert GABA to 2-pyrrolidone. The engineered C.g EAGAN2 strain was tested in a 5 L fermenter and after 72 h of fermentation, (8±0.3) g/L 2-pyrrolidone was accumulated. The 2-pyrrolidone synthesis pathway was for the first time established in C. glutamicum. The CoA transferase could convert GABA to 2-pyrrolidone, achieving optimum biosynthesis in fed-batch cultures using glucose as a cheap carbon source.
  • YAN Wenbin, ZHANG Xiaomei, SHI Jinsong, XU Zhenghong
    Food and Fermentation Industries. 2020, 46(11): 9-16. https://doi.org/10.13995/j.cnki.11-1802/ts.023625
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    L-serine is one of the 30 most important skeleton compounds in chemical and materials industries. In order to explore the mechanism of L-serine production by Corynebacterium glutamicum, the whole-genome sequencing and comparative genomics analysis were performed, and the relationship between genotype and phenotype was studied through reverse mutation, gene deletion and overexpression. The pK18mobsacB plasmid was used for reverse mutation of four genes (faS, folC, rhtA, and tyrP) related to transport or metabolism in the starting strain ΔSSAAI, and corresponding mutants Kf, Ko, Kr, and Kt were obtained. The fermentation of the mutants showed that, compared with the starting strain ΔSSAAI, the biomass of Kf, Ko, Kr, and Kt did not change significantly, and the L-serine titer of Kf and Ko did not change significantly, while that of Kr and Kt were 31.1 g/L and 31.04 g/L, increased by 18.5% and 18.3%, respectively. The deletion strains ΔrhtA and ΔtyrP were further constructed, and the fermentation results showed that there was no significant change in the L-serine titer. Then rhtA and tyrP were overexpressed in ΔSSAAI, L-serine titer improved slightly. rhtA and tyrP were overexpressed in themutantstrainA36,andtherecombinantstrainspD-RandpD-Twere obtained, resulting in L-serine titer of 32.55 g/L and 33.7 g/L, which was 6.5% and 10.3% higher than that of A36. At the same time, rhtA and tyrP overexpression resulted in a decrease of the biomass by 13.88% and 24.5%, respectively. The results indicated that rhtA and tyrP were related to the cell growth and L-serine production.
  • JIANG Cong, GUO Ailing, WEI Xuetuan
    Food and Fermentation Industries. 2020, 46(11): 17-22. https://doi.org/10.13995/j.cnki.11-1802/ts.023690
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    S-Adenosylmethionine (SAM) is highly important for human metabolism, and it can be used as a nutritional supplement to improve human health. During the de novo synthesis of SAM by Bacillus amyloliquefaciens, lipopeptide surfactants usually lead to energy loss and fermentation foaming, which may hinder the accumulation of SAM. In this study, the lipopeptide surfactants synthesis genes of ituD and srfAC were deleted and effects of these genes on SAM synthesis were investigated. The fermentation results showed that deletion of ituD had no significant effect on cell growth and SAM synthesis. On the other hand, deletion of srfAC promoted the SAM titer by 23%, and no significant effect was found on the cell growth. After double mutation of ituD and srfAC, the SAM titer was increased by 32%, and the cell growth was not affected significantly. This study confirms that blocked lipopeptide surfactants synthesis can significantly enhance the SAM production, which provides a new strategy for metabolic engineering breeding of SAM.
  • LI Leyun, LI Youran, XU Yinbiao, ZHANG Liang, GU Zhenghua, DING Zhongyang, SHI Guiyang
    Food and Fermentation Industries. 2020, 46(11): 23-31. https://doi.org/10.13995/j.cnki.11-1802/ts.023539
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    3,4-Dihydroxylphenylalanine (L-DOPA) is an analog of L-tyrosine (L-Tyr), which has broad application prospects in clinical medicine, cosmetic, health care and other fields. A gene (srth) encoding a tyrosine 3-monooxygenase from Streptosporangium roseum DSM 43021 was cloned and expressed in Bacillus licheniformis, and the recombinant srth was obtained. The intracellular and extracellular activities of the recombinant tyrosine 3-monooxygenase were 30.7 U/mL and 22.79 U/mL, respectively. The recombinant srth and a strain expressed sepiapterin reductase were efficiently used for production of L-DOPA from L-Tyr by whole-cell biotransformation. Tetrahydrobiopterin (BH4) was found a restrictive factor in the process and the ratio of the consumption of L-Tyr to the demand of BH4 was about 1∶2. The results provide a novel biotransformation routine for L-DOPA preparation.
  • LIU Jie, WANG Hongtao, QIAN He, XU Jianzhong, ZHANG Weiguo
    Food and Fermentation Industries. 2020, 46(11): 32-37. https://doi.org/10.13995/j.cnki.11-1802/ts.023242
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    In order to promote the industrial production of β-carotene, carotenoid-related genes were cloned from Sporidiobolus pararoseus and integrated into the genome of Pichia pastoris and a carotenoid-producing strain Pp-EYBI was constructed. Meanwhile, the gene copy number of the rate-limiting enzyme for β-carotene synthesis was optimized and the gene of MVA rate-limiting enzyme was over-expressed to enhance the supply of precursor materials and the flux to β-carotene synthesis. The strain Pp-EYBI+(YB)3H was finally obtained, which yielded as high as 3.7 mg/g (dry cell weight) β-carotene. This lays a foundation for the industrial production of β-carotene by P. pastoris.
  • ZHU Ruyi, HANG Feng, ZHANG Hao, LI Yuankun, ZHAO Jianxin, CHEN Wei, LU Wenwei
    Food and Fermentation Industries. 2020, 46(11): 38-45. https://doi.org/10.13995/j.cnki.11-1802/ts.023570
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    To explore the regularity and characteristics of biofilm formation of Bifidobacterium, the biofilm-forming ability of 114 strains distributing in six species of Bifidobacterium, including B. longum, B. breve, B. animalis, B. bifidum, B. adolescentis, and B. pseudocatenulatum, was analyzed by microplate assay. The results showed that according to the absorbance, Bifidobacterium was divided into three types: non-biofilm formers (OD600 nm≤1), weak biofilm formers (1<OD600 nm<3) and strong biofilm formers (OD600 nm≥ 3). All strains of B. bifidum were strong biofilm formers, while B.longum strains were not strong biofilm formers, 57.90% of which were non-biofilm formers. The biofilm-forming ability of other Bifidobacterium was different, including all three types of strains. It was found that the biofilm structures of B.animalis and B.bifidum were mushroom-shaped and all of other Bifidobacterium were flat using scanning electron microscopy, and the biofilms of B.longum and B.bifidum contained a higher proportion of viable bacteria using confocal laser scanning microscopy. The effect of surface properties of Bifidobacterium on the biofilm formation was performed using the adhesion of bacteria to the solvent. The results showed that the biofilm-forming ability of B. pseudocatenulatum was only related to the Lewis acid properties, B. breve, B. animalis and B. adolescentis were positively correlated with the surface hydrophobicity and Lewis acid properties, but there was no correlation between surface properties and biofilm formation for B. longum. The strains of B. bifidum with strong biofilm-forming ability, which exhibited high hydrophobicity and Lewis acid properties, had no relation between its surface properties to its biofilm-forming ability. In summary, the biofilm-forming characteristics of Bifidobacterium exerted species-specific and strain-specific effects and related to the surface characteristics.
  • LIU Lu, AI Lianzhong, XIA Yongjun, XIONG Zhiqiang, GUAN Tong, SONG Xin
    Food and Fermentation Industries. 2020, 46(11): 46-51. https://doi.org/10.13995/j.cnki.11-1802/ts.023272
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    The convenience and high efficiency make the enhanced green fluorescent protein (eGFP) widely used as a reporter in genetic manipulation. In order to screen strong promoters with high transcriptional activity in Lactococcus lactis NZ9000, a report system based on eGFP was constructed. Four promoters, including P11, P23, P32 and P44 were compared. The fragments of promoters P11, P32 and P44 were amplified by PCR using pLpCas9-0537, pMG36e or pNZ44 as template and cloned into pIB184-eGFP, respectively. The transcriptional activity of different promoters was compared by quantitative assay of eGFP using microplate reader. Quantitative assay of the fluorescence intensity showed the transcription activity of P11 was weak, and that of P23, P32 and P44 was 23.9, 7.8 and 4.1 times higher, respectively. Taken together, promoters with different transcriptional activity were screened out using the eGFP-based reporting system, which could be used as a basic tool for heterogenous gene expression in L. lactis.
  • HU Zicong, FANG Cuilan, LI Sheng, YE Fayin, ZHAO Guohua
    Food and Fermentation Industries. 2020, 46(11): 52-60. https://doi.org/10.13995/j.cnki.11-1802/ts.023531
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    This study aimed to evaluate the adsorption capacity and potential adsorption mechanisms of Cr (VI) by bamboo shoot shell acetic acid lignin (BSS-AL). The effects of pH, addition amount of BSS-AL, contact time and initial Cr(VI) concentration were studied through batch adsorption experiments. And the adsorption models were also been established. Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used to detect the functional groups and element composition of BSS-AL before and after adsorption in order to explore the adsorption mechanisms. The results showed that BSS-AL exhibited high-efficiency adsorption capacity of Cr (VI) and the removal rate of Cr (VI) could reach 97% in four hours. Furthermore, the pseudo-second-order kinetic model could be matched with the adsorption behavior over the whole range of adsorption process. Moreover, the adsorption of Cr (VI) by BSS-AL could be described well by Langmuir model and the maximum adsorption capacity reached 14.908 mg/g. The adsorption mechanisms could be explained by FTIR and XPS spectra: electrostatic interaction, ion exchange and re-adsorption after reduction. And BSS-AL has great potential to remove toxic pollutant Cr(VI) from food.
  • CHU Qianqian, AI Jian, FANG Jiaqi, HAN Qiuyu, BAO Bin, SUN Yongjun
    Food and Fermentation Industries. 2020, 46(11): 61-68. https://doi.org/10.13995/j.cnki.11-1802/ts.022733
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    This study aimed to investigate the interaction mechanism between phenolic compounds and proteins. The o-dihydroxybenzene (OP) and m-trihydroxybenzene (MP) were oxidized under alkaline conditions in the presence of oxygen to obtain oxidized o-dihydroxybenzene (OOP) and oxidized m-trihydroxybenzene (OMP), which were respectively reacted with oxidized and unoxidized OP, MP and BSA at room temperature. The changes of free amino, sulfhydryl, non-covalent interaction and surface hydrophobicity between BSA and OP, MP, OOP and OMP were analyzed. Changes were characterized by Fourier transform infrared spectroscopy (FTIR), circular dichroism (CD) and differential scanning calorimeter (DSC). The results showed that the content of free amino and sulfhydryl groups did not change significantly between BSA and OP and MP, but OOP and OMP could significantly reduce the content of free amino and sulfhydryl groups in BSA.AndthereductionofOOP-BSA was greater than that of OMP-BSA. Moreover, MP can form hydrogen bonds with BSA, but the ionic bond did not change significantly and the increase of MP-BSA hydrogen bond was greater than that of OP-BSA. After the reaction between OP and BSA, the hydrophobic interaction increased significantly. While MP caused the hydrophobic interaction to decrease significantly. Furthermore, OP, MP, OOP, OMP could significantly reduce the hydrophobicity of BSA surface and the reduction of OP-BSA, OOP-BSA was greater than MP-BSA, OMP-BSA. FTIR and CD results showed that OP, MP, OOP, OMP could change the secondary structure of BSA which was characterized by the decreased α-helix content and increased β-sheet, β-turn and random coil. The effects of OP and OOP were greater than those of MP and OMP. DSC results showed that the introduction of OP, MP, OOP and OMP increased the thermal denaturation temperature of BSA. These results indicated that catechol and phloroglucinol undergo oxidative interaction with BSA and unoxidized catechol and phloroglucinol could interact non-covalently with bovine serum albumin. These interactions may alter the functional properties of the protein, thereby affecting the application of the protein in food.
  • YU Fengsheng, SUN Zhigao, FANG Ming, MA Yaqin, SHENG Ran
    Food and Fermentation Industries. 2020, 46(11): 69-76. https://doi.org/10.13995/j.cnki.11-1802/ts.023658
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    The β-carotene was used to represent carotenoids, and its aqueous phase simulation system was constructed. The effects of endogenous factors such as sugar, acid, Vc and exogenous factors such as ultrasonic power, ultrasonic temperature on the degradation were investigated. The results showed that both Vc and citric acid could promote the degradation of carotenoids during ultrasound. And they had a synergistic effect when coexisting, sugar had a certain protective effect on carotenoids which could prevent the destruction of carotenoids by Vc and acid to varying degrees. The degradation kinetics of β-carotene under different ultrasound temperatures and different ultrasound powers were established respectively. It was found that the zero-order kinetic model was consistent only at 30 ℃, and the first-order kinetic model was consistent under other conditions. However, under different ultrasonic temperatures and ultrasonic power, the degradation rates were caused by thermal effects and ultrasonic cavitation effects, respectively. The β-carotene isomers were identified by HPLC-DAD and according to the Q value, the three isomers were determined to be 15-cis, 13-cis and 9-cis β-carotene. Fourier transform infrared spectroscopy showed that C—O and CO stretching vibration existed in the samples when treated by ultrasound. It could be preliminarily inferred that might be carotene epoxide, apocarotene aldehyde or apocarotene ketone. The volatile products produced by the degradation were analyzed by GC-MS which mainly were β-ionone, isophorone, 2,2,6-trimethyl cyclohexanone and the like.
  • HU Xiaolong, WANG Kangli, YU Miao, TIAN Ruijie, FAN Haibao, SUN Jixiang, ZHANG Jun, YANG Xu MA Geli, WEI Tao
    Food and Fermentation Industries. 2020, 46(11): 77-84. https://doi.org/10.13995/j.cnki.11-1802/ts.023541
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    Based on the high-throughput sequencing technology, the community diversity and its spatial heterogeneity of the prokaryotes harbored in pit muds locating at the top (PW-T), middle (PW-M), under layers (PW-U) of the pit wall and the bottom (PB), were analyzed. Fermentation pit muds were collected from a strong-flavor Baijiu company in Henan province. Firmicutes (71.1%) and Bacteroidetes (16.3%) were the dominant microorganisms, and a total of 78.1% (32 in total) of the dominant genera classified into classes of Clostridia or Bacteroidia, among which the Caproiciproducens was the predominant genus (27.3%) and there were a large number of unidentified genera (17.5%). The α- and β-diversity analysis showed that: (i) there were some spatial differences in pit-mud prokaryotic community diversity and species-abundance though not significant (P>0.05); (ii) there were spatial heterogeneities of pit mud prokaryotic community composition at the levels of phylum,class,genus and operational taxonomic units(OTU).Furthermore, redundancy analysis(RDA) showed that the pH and available phosphorus may be the main physicochemical factors affecting the spatial distribution of prokaryotic community in pit mud. The spatial distribution of pit mud prokaryotic community diversity and its influencing factors were systematically studied in this work, which could provide a theoretical basis for exploring the specific effects and contribution of pit muds locating in different zones of fermentation pit for the strong-flavor Baijiu production, as well as enriched the content of pit mud prokaryotic community diversity in Henan Baijiu production area.
  • WANG Ruyue, YU Xun, XU Jingjing, ZHU Li, ZHAN Xiaobei, ZHANG Hongtao
    Food and Fermentation Industries. 2020, 46(11): 85-91. https://doi.org/10.13995/j.cnki.11-1802/ts.023556
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    Oat β-glucans (OGs) and the resultant oligosaccharides (oats β-glucan oligosaccharides, OGOs) are considered to be functional components with potential prebiotic properties. OGs with a molecular weight of 1.34×105 Da was extracted from oat bran, and OGOs with polymerization degree ranging from 3 to 11, were obtained from acid hydrolysis of OGs. Using OGs and OGOs respectively as the carbon source, in vitro anaerobic fermentation was conducted using fecal flora from healthy people or type 2 diabetic patients. The effects of OGs and OGOs on composition and metabolism of intestinal flora from different sources were studied. The results showed that both fecal floras could make good use of OGs and OGOs, and the utilization rate reached over 93% after 48 h fermentation. The pH value of the fermentation broth significantly decreased, and the content of acetic acid and propionic acid significantly increased after 48 h fermentation. Compared with OGOs, the ratio of butyrate-producing bacteria significantly increased (P<0.05) using OGs as carbon source, leading to a significantly increased (P<0.05) butyric acid content in the fermentation broth. Compared with OGs, using OGOs as a carbon source significantly increased the relative contents of Lactobacillus spp. and Bifidobacterium spp. in the fecal flora (P<0.05). Also, the content of acetic acid and propionic acid was significantly increased in the fermentation broth. Based on the results, OGs and OGOs have different effects on the composition and metabolism of the intestinal flora. And OGs and OGOs may specifically improve the composition and related metabolism of the intestinal flora in type 2 diabetic individuals.
  • XU Hui, JIA Lina, SU Jingyi, XU Lili, ZUO Shuna, ZHANG Wei, YANG Qian
    Food and Fermentation Industries. 2020, 46(11): 92-97. https://doi.org/10.13995/j.cnki.11-1802/ts.023441
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    The toxicity mechanism of patulin (PAT) on human gastric epithelial cells (GES-1) was studied in vitro from the aspects of cell proliferation, apoptosis, oxidative damage and DNA damage. When the concentration of PAT was above 1.0 μmol/L, the inhibition rate of PAT on GES-1 cells was greater than 20%. Meanwhile, the obvious apoptosis phenomenon was observed under fluorescence microscope. The relative expression level of Bax mRNA showed a significant up-regulation, whereas the relative expression level of Bcl-2 mRNA was distinctly down-regulated. At the same time, the activity of SOD and CAT decreased at most by 53.76% and the content of GSH reduced at most by 56.55%. The content of MDA increased at least by 139.59%. The expression of γ-H2AX was elevated with the increasing concentration of PAT. The results showed that PAT could significantly inhibit the proliferation and induce the apoptosis of GES-1 cells, with significant dose-effect relationship. It is concluded that PAT may induce apoptosis of GES-1 cells through oxidative damage and DNA damage.
  • WU Meitong, ZHANG Haixin , ZHANG Meng, BI Yuan, XU Haoran, LING Kong, JIN Qiguan, LIU Wenying
    Food and Fermentation Industries. 2020, 46(11): 98-104. https://doi.org/10.13995/j.cnki.11-1802/ts.023063
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    To investigate the inhibitory effect and mechanism of oyster enzymatic hydrolysate (OEH) on the growth of Lewis lung cancer, 48 male C57BL/6J mice were randomly divided into four groups: C, LOEH, MOEH and HOEH. Subcutaneous Lewis lung cancer model was established in mice and OEH was supplemented at low, medium and high doses. Five weeks later, the volume and weight of the tumor were measured, the structure of tumor tissue was observed, the rate of apoptosis and necrosis of lung cancer cells in lung cancer tissue, the percentage of CD3+, CD4+, CD8+ T-lymphocyte, natural killer cells (NK), and regulatory T lymphocyte(Treg) in blood also were measured. The results showed that compared with group C, the weight of tumors in MOEH and HOEH groups decreased significantly (P=0.007, P=0.013); the apoptosis rate of lung cancer cells and the percentage of CD3+ increased significantly (P=0.001, P=0.024) and the percentage of Treg in LOEH group decreased significantly (P=0.000); the rate of necrosis (P=0.009, P=0.014) and apoptosis (P=0.010, P=0.001) of lung cancer cells increased significantly and the percentage of Treg in MOEH and HOEH groups decreased significantly (P=0.000, P=0.008); the percentage of CD4+, CD4+/CD8+ ratio, NK cells in MOEH group increased significantly (P=0.000, P=0.016, P=0.05), and CD4+, NK cells in HOEH group increased significantly (P=0.024, P=0.044). OEH can significantly inhibit the growth of lung cancer by improving the function of T-lymphocytes and NK cells to increase the apoptosis and necrosis of lung cancer cells in tumor bearing mice.
  • LI Guanlong, LIU Xiaolan, ZHENG Xiqun
    Food and Fermentation Industries. 2020, 46(11): 105-111. https://doi.org/10.13995/j.cnki.11-1802/ts.023633
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    In recent years, the increasing incidence of thrombotic diseases has seriously threatened human life and health. Some functional compounds in foods can increase the activity of human fibrinolytic system, thus gradually restore human's normal physiological state and reduce the tendency of thrombophilia. A novel fibrinolytic enzyme was purified from the fruiting body of Agrocybe aegerita by ammonium sulfate precipitation, Sephadex G-25 gel filtration, CM-Sepharose FF ion exchange, Source 15PHE hydrophobic chromatography and Mono S ion exchange. The fibrinolytic enzyme had a specific activity of 1 731.05 U/mg, with the purification fold of 246.94. The enzyme was named ACase and comprised two subunits. The molecular weights of the two subunits were estimated to be approximately 31.4 kDa and 21.2 kDa, respectively, by SDS-PAGE. Only one band appeared on Native-PAGE and it was found to show fibrinolytic activity. The analysis by Edman degradation method showed that the N-terminal sequences of the two subunits were AIVTQTNAPWGL and SNADGNGHGTHV, respectively. The enzyme was identified as a newfibrinolyticenzymebysequencecomparisoninGenBank. ACase could degrade the three chains (α, β and γ) of fibrinogen in order and exhibited the functionality of anticoagulant in vitro. Agrocybe aegerita has the potential application as functional food ingredient in food industry.
  • ZHANG Dongxia, ZHANG Qi, DENG Liling, ZHONG Geng
    Food and Fermentation Industries. 2020, 46(11): 112-118. https://doi.org/10.13995/j.cnki.11-1802/ts.023678
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    The possibility of intestinal prebiotics of konjac glucomannan (KGM) and konjac oligo-glucomannan (KOGM) in treatment of lactose intolerance was studied both in vivo and in vitro. According to the in vitro fermentation of fecal flora from healthy and lactose intolerant subjects, compared to the control, pH value of fermentation broth with KGM and KOGM addition decreased by 1.46-1.56, whereas the output of short-chain fat acids (SCFA) increased significantly (P<0.05). Meanwhile, five weeks old KM mice were given KGM and KOGM for four weeks; the pH value of mice feces decreased while the water content and SCFA concentrations increased significantly (P<0.05). The effect of polysaccharide on lactose fermentation in vitro showed that the pH value in fermentation cultures with KGM and KOGM addition decreased by 2.70-2.83 compared to the control after 24 h. However, the content of acetic acid, butyric acid and lactase activity were increased significantly (P<0.05) by 9.3-17.6 mmol/L, 2.1-2.9 mmol/L and 0.001-0.002 U/mg, respectively. These results indicated that KGM and KOGM could provide a suitable acidic environment that could effectively improve the metabolism of intestinal microflora in mice to produce SCFA. As natural and safe ingredients, KGM and KOGM could increase the activity of lactase, and had a potential usage to treat lactose intolerance.
  • CHEN Senyi, LIU Zhenmin, JIAO Jingkai, BAO Yi, PANG Jiakun, DANG Huijie
    Food and Fermentation Industries. 2020, 46(11): 119-124. https://doi.org/10.13995/j.cnki.11-1802/ts.022997
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    Lactic acid bacteria combinations were screened for fresh cheese preparation by evaluating the quality of the product during 4 ℃ storage. Lactobacillus lactis E11 and C44, Lactobacillus helveticus G12, Lactobacillus casei D41, Lactobacillus paracasei B1, Enterococcus faecium F22 and N were used in six different combinations. Combination-1 (E11∶C44∶F22=1∶1∶1) in milk fermentation revealed the cheese with the fastest acid production, moderate viscosity elevation and relatively higher water holding capacity. The yield of the cheese was as high as 31.42% with Combination-1, and the L* value is 91.70±0.02, significantly higher than the control group. Moreover, its total score in sensory evaluation was higher than that of the control group with improved textural and viscoelastic properties. Combination-1 starter has potential application for fresh cheese production.
  • YU Zhicheng, HUANG Fuqi, XU Zhou, CHENG Yunhui
    Food and Fermentation Industries. 2020, 46(11): 125-130. https://doi.org/10.13995/j.cnki.11-1802/ts.023574
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    In order to effectively utilize the protein resources in soy sauce residue, hydrolysis was performed with alkaline protease followed by the assay of immune activity, and the preparation process were optimized through response surface test. The effect of enzymatic hydrolysate on NO release in cells of lipopolysaccharide (LPS) inflammation model was used as an index. The optimal conditions for enzymatic hydrolysis were obtained: substrate concentration 5%, pH 7.5, 61.31 ℃, enzymolysis time 2.86 h, and enzyme dosage 5 468 U/g, the inhibition rate of NO release in rat macrophages could reach 18.92%. Based on the industrial production, the adjusted process conditions are: substrate concentration 5%, pH 7.5, 60 ℃, enzymatic hydrolysis time 2.9 h, and enzyme dosage 5 500 U/g. Soy sauce residue protein alkaline protease hydrolysate has better immune activity, and the optimized process conditions are feasible.
  • YIN Lebin, ZHOU Juan, HE Ping, LIAO Cong, YANG Ailian, LIU Dan, LI Licai
    Food and Fermentation Industries. 2020, 46(11): 131-137. https://doi.org/10.13995/j.cnki.11-1802/ts.023857
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    Using the supernatant from the waste-water produced in soybean product processing as raw material, the soybean active peptide was prepared by lactic acid bacteria fermentation and its antioxidant activity was studied. Taking peptide yield as evaluation index, based on the results from single factor test, a response surface software was used to establish a mathematical model for the process of preparing peptide from fermented soybean supernatant by lactic acid bacteria. The EC50 for peptide scavenging of ·OH, ABTS and DPPH radical activity was determined. ANOVA results showed that the order of affecting the peptide yield was: bacteria addition amount> fermentation temperature> initial pH> fermentation time. Response surface model regression analysis showed that the optimal fermentation conditions were as follows: inoculation 4.71% (4.71×108 CFU/100 mL), initial pH 6.44, 40.21 ℃, and fermented for 11.66 h. The predicted peptide yield was (56.97±0.27)%. Adjusting the fermentation parameters in combination with the specific conditions of the experiment, then the verified peptide yield was (57.33 ± 0.32) %, which was not significantly different from the predicted value (P> 0.05). The results of in vitro antioxidant tests showed that the EC50 of the ·OH, ABTS, and DPPH free radical scavenging by the peptide was 17.84, 1.14 and 7.61 mg/mL, respectively, which were 1.2, 4.75 and 2.16 times higher than the value before fermentation. The research provided experimental basis for the high value development and utilization of soybean supernatant.
  • WANG Bixiang, HE Yang, JIANG Haiqin, WEN Liankui
    Food and Fermentation Industries. 2020, 46(11): 138-145. https://doi.org/10.13995/j.cnki.11-1802/ts.023479
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    To investigate the production of short-chain fatty acids (SCFAs) from soybean insoluble fiber (SIDF) in fermentation, SIDF was fermented by Lactobacillus sp., Bifidobacterium bifidum, Enterococcus faecalis, and Escherichia coli, respectively. The pH value of the fermentation broth was measured, and the content of SCFAs were determined by gas chromatography at different time points. In fermentation of SIDF by 4 exogenous intestinal bacteria, the pH value of the fermentation broth first decreased, and then remained stable after 24 h fermentation. During the fermentation by 4 exogenous intestinal bacteria, the yield of acetic acid was the highest , followed by propionic acid and butyric acid. The content of acetic acid and propionic acid produced by B. bifidum each reached its maxima after 24 h fermentation, which was (4.05±0.024) g/L and (0.13±0.014) g/L, respectively. The content of butyric acid produced by Lactobacillus sp. was the maxima of (0.082±0.001) g/L after 24 h fermentation. The total SCFAs produced by B. bifidum fermentation was the highest. SIDF was fermented by exogenous intestinal bacteria to produce SCFAs, and the content of SCFAs produced varied with the fermentation time. B. bifidum had the strongest ability to produce SCFAs by fermenting SIDF.
  • XING Hongbo, XU Ganrong, NI Dongjiao, ZENG Li, MO Jinling, ZOU Xinhua
    Food and Fermentation Industries. 2020, 46(11): 146-150. https://doi.org/10.13995/j.cnki.11-1802/ts.023224
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    The stability of Monascus monascorubrin and monascin from Monascus sp. was studied by spectral scanning and thin layer chromatography (TLC). It was showed that monascorubrin and monascin were sensitive to light and their color value decreased with time. TLC results revealed the conversion of some monascorubrin into monascin during the process. When monascorubrin and monascin fermentation broth was mixed directly with food ingredients, the color of the protein-containing materials gradually changed from orange/yellow to red, while that of the protein-free materials not. When protein-containing materials were mixed with the monascorubrin and monascin fermentation broth, spectral scanning analysis revealed deviation of the wavelength scanning spectrum and TLC showed the disappearance of monascorubrin and the formation of new red pigment. Monascorubrin and monascin showed good thermal stability.
  • LI Shuai, AI Lianzhong, ZHANG Hui, SONG Zibo, LAI Phoency
    Food and Fermentation Industries. 2020, 46(11): 151-156. https://doi.org/10.13995/j.cnki.11-1802/ts.023879
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    The aim of this study was to increase the value of valorize passion fruit peels(PFP). PFP was extracted by acid and then modified with octenyl succinic anhydride (OSA) to yield pectin octenyl succinate anhydride (POSA). The zeta potential, particle size, rheological properties, and microstructure of PFP and POSA were examined and compared with commercial citrus pectin (CP) to illustrate the stabilizing effects of PFP and POSA on acidified milk drink (AMD). The results show that the addition of PFP, POSA, or CP at 2.0 g/L could increase the absolute value of the ζ-potential at pH 4.68, reduce the effective particle size and prevent the formation of milk protein aggregates. The stability effect was POSA>PFP>CP. Besides, PFP and POSA also could improve the shear resistance, viscoelastic moduli (G′ and G″) of the AMD emulsion, accompanying with changes in gel properties. These results provided a theoretical basis and a perspective for passion fruit pectin. And its OSA derivative could be potentially used as stabilizers in food industries in the future.
  • XIE Guangfa, CAI Jihao, QIAN bin, WANG lan, LU Zhendong, LYU Fei, DING Yuting
    Food and Fermentation Industries. 2020, 46(11): 157-164. https://doi.org/10.13995/j.cnki.11-1802/ts.023469
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    The changes of volatile components, organic acids and free amino acids during brewing process of dry Huangjiu were studied, and the characteristic aroma compounds in different stages of the brewing process were determined by principal component analysis. A total of 48 volatile components, 7 organic acids and 16 free amino acids were detected. Among them, the volatile aroma compounds increased rapidly in the first 4 days and became stable in the later period, reaching 344.63 mg/L. The contents of organic acids and free amino acids increased throughout the brewing process, reached 4 583.2 mg/L and 2 234.4 mg/L, respectively, in 18 days. In the early stage, the characteristic aroma compounds were mainly ethyl benzoate, isoamyl alcohol, phenylethyl acetate, phenylethanol, aspartic acid, threonine, etc. In the later stage, they were mainly ethyl acetate, ethyl lactate, lactic acid and arginine.
  • YAN Hejing, ZHANG Mingyu, SUN Kang, YANG Xiaokuan, GE Chao, ZHOU Jiefang, LIU Chang, ZOU Jing
    Food and Fermentation Industries. 2020, 46(11): 165-171. https://doi.org/10.13995/j.cnki.11-1802/ts.023634
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    Fermentation with pure active dry yeast easily causes homogenization of the wine. Controlled fermentation with selected non-Saccharomyces yeast and S. cerevisiae can highlight the varietal and regional characteristics of the wine. The objective of this study is to evaluate the effects of the co-fermentation using indigenous S. cerevisiae HBKS-Y1 and H. uvarum HBKS-Y3, which were isolated from Muscat grape spontaneous fermentation, on the fermentation process and aroma of Muscat wine. Four types of fermentations were performed, pure fermentation was inoculated with only one strain (active dry yeast FX10, HBKS-Y1 or HBKS-Y3), and mixed fermentation was simultaneously inoculated with HBKS-Y1 and HBKS-Y3. All the fermentations were performed in sterilized Muscat grape juice. During fermentation, the samples were taken for composition analyses and yeast population calculation. The population of S. cerevisiae and H. uvarum were calculated with yeast extract peptone dextrose medium (YPD) and lysine agar plate, respectively. The volatile components of the four wine samples were measured by GC-MS, and sensory analysis of the wine was conducted by 10 special panelists. The results indicated that wild yeast HBKS-Y1 and HBKS-Y3 could complete the fermentation independently, and there was interaction between HBKS-Y1 and HBKS-Y3 in the mixed fermentation process, which reduced the amount of higher alcohols from HBKS-Y1 fermentation and inhibited the production of esters and acids by HBKS-Y3. More importantly, the mixed fermentation increased the content of 2-phenylethanol, terpene and monoterpene alcohols, which are typical aroma components of Muscat wine. According to sensory evaluation, the total acceptability of mixed fermented wine was similar to that fermented by FX10, while the score of flower fragrance and rose fragrance were both the highest. Mixed fermentation of wild yeast HBKS-Y1 and HBKS-Y3 revealed positive effect in highlighting the varietal characteristics of Muscat wine.
  • ZHANG Guangen, CHEN Pengcheng, ZHENG Pu, WU Dan, QIAO Hui, ZHANG Wenyi, JIANG Sufei
    Food and Fermentation Industries. 2020, 46(11): 172-177. https://doi.org/10.13995/j.cnki.11-1802/ts.023249
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    A strain for efficient removal of amine nitrogen was isolated from the sludge using 96-well plate screening and shake flask fermentation. The strain was identified as Bacillus amyloliquefaciens by morphology, physiology and 16s rDNA identification. The nitrogen-removing properties were investigated. It was able to remove ammonia and nitrite by assimilation with the removal rate for ammonia of 92.32% when the ammonia concentration was 100 mg/L, and the removal rate for nitrite of 100% when the nitrite concentration was 50 mg/L. The strain removed ammonia at higher speed under higher ammonia concentration and could effectively remove ammonia under as high as 3.2 g/L organic nitrogen. Optimization of spore-producing fermentation was carried out in shake flask, and a spore concentration of 2.3×109 CFU/mL was obtained when C:N (mass ratio) was 1.32. In a 3 L fermenter, the spore concentration reached 1.12×1010 CFU/mL in 24 h fed with 10 mg/(L·h) of glucose. The fermentation broth was mixed with corn cob powder and could remove ammonia fast and efficiently under different initial concentrations of ammonia in simulated aquaculture water. The average removal rate of ammonia was 3.92 mg/(L·h), outstanding ability to improve the water quality in aquaculture.
  • ZHAO Xueping, WEN Yajiao, LI Zhengying, ZHENG Haiwu, HUANG Haiying, LI Xiaojuan
    Food and Fermentation Industries. 2020, 46(11): 178-183. https://doi.org/10.13995/j.cnki.11-1802/ts.022065
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    In order to select excellent local yeasts for wine brewing in Wuhai area of Inner Mongolia, 13 yeast strains were isolated and purified from local orchard soil, grape skins as well as the fermentation mash. Finally, a strain named KX3 was obtained and it was identified as Saccharomyces cerevisiae in Saccharomyces spp. after 26S rDNA D1/D2 screening and sequencing. Afterwards, the tolerance and sensory tests were conducted on the fermented broth(wine) using KX3 as the starter. The results indicated that the alcohol tolerance of the strain KX3 was 16%, SO2 tolerance was 400 mg/L, sugar tolerance was 500 g/L, and pH tolerance was 2.5. In comparison with commercial Saccharomyces cerevisiae CSM and CECA, the KX3 obtained better growth characteristics. Therefore, it was confirmed that KX3 could be used for wine brewing since it simultaneously possesses good growth characteristics and harmonious fruit aroma.
  • WANG Xuesong, XIE Jing
    Food and Fermentation Industries. 2020, 46(11): 184-190. https://doi.org/10.13995/j.cnki.11-1802/ts.023622
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    The effects of different freezing methods on the quality of horse mackerel were studied. Four different quick-freezing methods (flat freezing, spiral freezing, quick-freezing refrigerator freezing, and salt solution immersion freezing) were used to freeze fresh horse mackerel. The changes of physical and chemical indicators were analyzed to evaluate the quality change of fish meat including water retention, conductivity, texture, high-speed iron, dialdehyde content, sulfhydryl content and protein tertiary structure. Furthermore, the water migration and microstructural changes in fish meat were also detected by low-field nuclear magnetic resonance and optical microscope. The results showed that the time for salt immersion freezing, spiral freezing, quick-freezing refrigerator freezing, and plate freezing to pass through the maximum ice crystal formation zone were 6 min, 39 min, 51 min, and 78 min respectively. Salt solution immersion freezing needed the shortest time, but protein denaturation and fat oxidation were longer. The qualities of horse mackerel by spiral freezer frozen were closest to those of fresh fish in terms of water retention, conductivity, texture, protein and fat quality, and water migration, and the muscle fiber gap was compact. Therefore, compared with other freezing methods, spiral freezing was beneficial to maintain the quality of horse mackerel.
  • WANG Debao, MA Wenshu, WANG Bohui, ZHANG Junying, KANG Lianhe, WANG Limei, ZHANG Yuanyuan, QIAO Jianmin, LI Xingyun, WANG Xiaodong, GUO Tianlong
    Food and Fermentation Industries. 2020, 46(11): 191-198. https://doi.org/10.13995/j.cnki.11-1802/ts.023187
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    To investigate the effects of different ripening time on the edible quality, lipid antioxidant and the formation of flavor substances of oat mutton sausage. Fermented oat lamb sausage was prepared by inoculating Lactobacillus sakei and Staphylococcus xylosus. The results showed that the relative content of volatile flavor substances in sausage increased first and then decreased. The composition and content of flavor substances were aldehydes, alcohols, terpenes, esters and acids, among which aldehydes accounted for more than 50% of the total flavor substances. When processing for 4 days, the relative content of flavor substances in sausage was significantly higher than that at the end of ripening (day 7), fermentation (day 1), and pickling (day 0). The content of caproate and caprylate, which presented fruit fragrance, also increased slightly at day 3 ripening. At 3 days' maturity, the value of lipid peroxidation decreased to 0.39 mg MDA/kg, with no significant difference from day 0. The redness value of sausage (a*) first rose and then decreased, and reached the peak at the end of fermentation, then it decreased to 45.47 after 3 days of maturation, with no significant difference from fermentation end point .The pH value of sausage was 4.89 at 3 days' maturity, which was significantly lower than the safe acidity(5.30) of fermented meat products. At this time, the water activity (aw) decreased to 0.83, which was lower than that required by the growth of most microorganisms and the expression of enzyme activity. After 3 days′ maturity, the relative contents of caproaldehyde, heptyl aldehyde, caproate, caprylate and terpene in sausage increased, lipid oxidation reduced, and the redness value of sausage improved. It was concluded that the maturity of three days after fermentation is the best process selection end point to improve the quality of fermented oats sausage.
  • GU Wenjing, JIN Yingshan, YERIM Jung, CHENG Yudong, JIN Yinzhe
    Food and Fermentation Industries. 2020, 46(11): 199-209. https://doi.org/10.13995/j.cnki.11-1802/ts.023555
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    The changes in the electrical conductivity (EC) and taste nucleotides of Litopenaeus vannamei shrimp with the treatment of ohmic heating (OH) were investigated in order to investigate the effect of OH on the taste of Litopenaeus vannamei. The EC (50 Hz~20 kHz) and the contents of nucleotides in the shrimp under different temperatures (25, 40, 55, 65, 75, 85, and 95 ℃) and metal ion additives (1, 5, and 10 mmol/L Zn2+, Cu2+, Al3+, Fe3+) were measured. The results show that the EC of minced shrimp has a logarithmic relationship with frequency and a linear relationship with temperature (lower than 65 ℃) (P<0.05). The EC of shrimp with added metal ions was higher than that of pure shrimp and 10 mmol/L Fe3+ was the best conductor for shrimp. In the treatment of OH, the guanosine monophosphate(GMP) content was always at a high level (more than 60 mg/100 g), while the Hypoxanthine(Hx) and Inosine(HxR) contents were always at a low level (less than 7 mg/100 g). Adenosine(AdR) was accumulated at high temperatures. The best temperature of heating for pure shrimp by OH was between 55~65 ℃, which was conducive to the production of umami taste. The shrimp treated with metal ions produced a small amount of Hx and a large amount of inosine monophosphate(IMP) at different temperatures. There was no significant correlation between IMP production and metal ion concentration (P>0.05). The IMP content in shrimp was the highest (201.39±3.83 mg/100 g) when 10 mmol/L Fe3+ was added and ohmic heated to 85 ℃. This study provided the optimized treatment condition for Litopenaeus vannamei using OH.
  • LUO Wentao, WANG Ziyi, PENG Binqian, SHEN Xiaofang
    Food and Fermentation Industries. 2020, 46(11): 210-215. https://doi.org/10.13995/j.cnki.11-1802/ts.023552
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    In order to reduce the oxidation of unsaturated fatty acids in chia seed oil and enhance its antioxidant properties, herein, chia seed oil microcapsule was prepared by spray drying technology. Soy protein isolate and maltodextrin, chia seed oil, glyceryl monostearate and sucrose fatty acid ester were used as wall, core material and emulsifiers respectively. The embedding conditions were optimized using microcapsule encapsulation efficiency as an indicator. The results showed that the optimized process conditions including mass ratio of core-to-wall material, mass ratio of soy protein isolate to maltodextrin, emulsifier addition, and solid content were 1∶3, 1∶2, 1.0%, 30%, respectively. The as-prepared microcapsule was spherical and uniformly distributed. Moreover, the encapsulation efficiency was as high as 93.79% and it had moisture content of 1.33%. Chia seed oil microcapsule had certain thermal stability, which could cope with the conventional food processing processes. Thus, the application scenario of chia seed oil is widened; it could be further developed and applied in yogurt, baked products, or functional food.
  • HUANG Jianzhao, LI Pan, XU Jinwei, LIN Huaina, XIE Lanhua, DU Bing
    Food and Fermentation Industries. 2020, 46(11): 216-222. https://doi.org/10.13995/j.cnki.11-1802/ts.023470
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    In order to improve the stability of sacha inchi oil in the food and reduce the quality risk of oxidative deterioration, sacha inchi oil nanoemulsions were prepared by high pressure homogenization method. Based on average particle size, polydispersity index (PDI), zeta potential, and split ratio index (SRI), the optimum process parameters were determined as follows: homogenization pressure 90 MPa with homogenization for five times; and concentration of emulsifier and sacha inchi oil were 2.5% and 5% respectively. With the treatment of the optimized conditions, the average particle size of nanoemulsion was (169.33±7.60) nm and PDI was (0.115±0.02). Moreover, zeta potential was (-56.8±0.56) mV and SRI was (0.12±0.02), respectively. Furthermore, the storage stability analysis at 4 ℃ and room temperature for 90 days showed that 5% and 10% sacha inchi oil nanoemulsions showed excellent physical and storage stability. And after 90 days of storage, the average particle size only increased by 18.67 nm. These results provided ideas for new product development of sacha inchi oil nanoemulsions and promoted the application of sacha inchi oil in food processing.
  • HOU Yaling, ZHU Wenxue, SUN Guofeng, BAI Xiting, MA Yitong
    Food and Fermentation Industries. 2020, 46(11): 223-230. https://doi.org/10.13995/j.cnki.11-1802/ts.023291
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    In order to study the strengthening effect of ultrasound on vacuum-dried whole egg liquid, the ultrasonic and vacuum drying methods were used to investigate the drying characteristics and quality of egg powder obtained after drying under different drying temperature, ultrasonic sound energy density and ultrasonic time. The results showed that with the increase of drying temperature, ultrasonic sound energy density and ultrasonic time, the vacuum drying rate of whole egg liquid increased. Except for emulsification stability, the effect of drying temperature resulted in other properties first increased and then decreased. And the optimal drying temperature was 50 ℃. With the increase of ultrasonic acoustic energy density and ultrasonic time, the preservation rate, stability coefficient, solubility and foam stability of soluble protein in egg powder gradually increased, while the foaming, emulsification and emulsification stability increased initially and then decreased. The best ultrasonic sound energy density and ultrasonic action time were 1.2-1.6 W/g and 2-2.5 h, respectively. This result could provide a theoretical basis for the production and quality control of ultrasonic vacuum drying of whole egg powder.
  • ZHANG Fangming, TENG Haihui, JIN Yongxue, LI Huimin, ZHENG Tao, ZENG Yiqiong, ZHENG Hui, YANG Yong
    Food and Fermentation Industries. 2020, 46(11): 231-237. https://doi.org/10.13995/j.cnki.11-1802/ts.023520
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    In order to study and evaluate the polyphenols and antioxidant activity in brown sugar of traditional technology, the extraction methods of total polyphenols and their antioxidative effects in vitro were investigated. Based on the Folin phenol method, this research applied response surface analysis method to optimize the optimal extraction conditions of total polyphenols in brown sugar with the concentration of ethanol, the ratio of liquid to material, the extraction temperature and the extraction time as factors. Moreover, the total polyphenol content and antioxidant capacity of different batches of brown sugar were determined and the differences of polyphenol compounds were qualitatively analyzed by high performance liquid chromatography (HPLC). Results showed that the optimized extraction conditions were as follows: the concentration of ethanol and the ratio of material to liquid were 52.78% and 25.54∶1(mL∶g) respectively. Furthermore, the optimized extraction temperature was 40 ℃ for 44.82 min. Besides, the antioxidant capacity of different batches of brown sugar polyphenols was positively correlated with the total polyphenol content while the types of polyphenols of different batches of brown sugar were the same, but there were significant differences in specific polyphenol content among different batches.In conclusion,the extraction conditions of polyphenols were optimized. The brown sugar of traditional technology contains certain polyphenols and has strong antioxidant capacity. Polyphenols should be one of the important functional components of brown sugar of traditional technology.
  • ZHENG Xiaojie, LIN Shengli, WU Congcong, Nie Xiaohua
    Food and Fermentation Industries. 2020, 46(11): 238-243. https://doi.org/10.13995/j.cnki.11-1802/ts.023297
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    In order to explore the appropriate harvest time, the variation of antioxidant capacity and phenolic compounds in the leaves from 5-year-old C. paliurus trees of the same cultivar across two cultivation sites (Wencheng, WC; Zhangjiajie, ZJJ) were investigated. DPPH and ABTS scavenging ability, reducing power, total phenolic and flavonoid contents of WC leaves increased initially then decreased as the season progressed. Whereas antioxidant capacity and total phenolic content showed a distinct bimodal pattern, along with a slight variation in total flavonoid content except for September. Two phenolic acid and ten flavonoids were qualitatively identified in the extracts of C. paliurus leaves. Among them, (+)-catechin was detected in WC leaves, but not in ZJJ leaves. And the content of 2,3-O-dicaffeoyl tartaric acid was highly positively correlated with the tested antioxidant capacities (r=0.917-0.941). The results suggested that the appropriate harvest time of C. paliurus leaves, despite the same cultivar, differed across the cultivation site, and 2,3-O-dicaffeoyl tartaric acid might act as an important phenolics for antioxidant capacity of the eaves. It would provide the basis for quality control and product development of C. paliurus leaves.
  • GU Saiqi, ZHANG Chenchao, ZHANG Yueting, BAO Rongbin, LIN Zhaoyong, ZHOU Xuxia, DING Yuting, ZHOU Zhenyi, YANG Jing
    Food and Fermentation Industries. 2020, 46(11): 244-251. https://doi.org/10.13995/j.cnki.11-1802/ts.023706
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    Quality and flavor changes of Pneumatophorus japonicus, Collichthys lucidus, and Harpadon nehereus during ice storage were examined. The results showed that the total number of colonies, total volatile base nitrogen value, and thiobarbituric acid value of the three kinds of marine fish increased continuously with the increase of ice storage time. The growth rate of Pneumatophorus japonicus was faster than the other two kinds of marine fish, and Harpadon nehereus was the slowest. Moreover, the odor scores of three kinds of marine fish were lower than 60 by the 8th day of storage, which indicates that the overall smell of the fish samples was unacceptable. The pH value decreased at first and then increased. And pH values of Pneumatophorus japonicus<Collichthys lucidus<Harpadon nehereus in the same storage time. A total of 32 volatiles were detected using GC-MS and 15 odor-active-compounds, such as isovaleraldehyde, were identified. Based on principal component analysis, hexanal and 1-octene-3-ol were the characteristic volatiles of "fresh" marine fish, whereas isovaleraldehyde, glutaraldehyde, nonanal, styrene, trimethylamine, and dimethyltrisulfide were identified as the characteristic volatiles of "deterioration" fish. This study could provide a basis for the relevant enterprises or departments to develop a rapid evaluation method of aquatic product quality in the future.
  • LI Wei, ZHANG Xiaoying, CHEN Rong, YE Jiayi, MIAO Jianyin, CHEN Yunjiao, CAO Yong
    Food and Fermentation Industries. 2020, 46(11): 252-257. https://doi.org/10.13995/j.cnki.11-1802/ts.022781
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    Preservative effects of chilled pork treated with polyphenols in Eucalyptus robusta leaves polyphenols extact (EPE) were studied. The chilled pork was treated with EPE at different concentrations. Meat color, MetMb content, MDA, TVB-N content, drip loss and sensory index of pork at 0, 3, 6, 9 and 12 d were determined respectively. The results showed that compared with the control group, EPE groups significantly increased a* value and sensory evaluation score of the chilled pork (P<0.01). Meanwhile, the MetMb content, TVB-N content, MDA value, pH value and drip loss of chilled pork during storage (P<0.01) were decreased, which effectively improved the quality of chilled pork. This indicated that PE could have a good antioxidant effect on chilled pork, which was beneficial to maintain the stability of meat color during storage.
  • ZHAO Yazhu, HAO Xiaoxiu, MENG Jie, ZHANG Ran, LIU Guangfa
    Food and Fermentation Industries. 2020, 46(11): 258-263. https://doi.org/10.13995/j.cnki.11-1802/ts.023399
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    In order to explore the antimicrobial activity of different components in thyme essential oils, three thyme essential oils were selected and analyzed by GC-MS, and their effects on Botrytis cinerea, Penicillium sp., Alternaria sp., and Rhizopus sp. were evaluated. Antimicrobial packaging cartons were prepared by A corrugated cardboard coated with nano-emulsion made of selected thyme essential oils, soy protein isolate, and Tween 80. The strawberries were stored in the cartons at (26±2) ℃, RH (45±5)%. The preservation effect was evaluated by examining the sensory quality, decay rate, total number of colonies, weight loss rate, and soluble solids of strawberries. The results showed that the total percentages of thymol and carvacrol in thyme essential oils were 16.03%, 27.09%, and 75.62% for samples No. 1, 2, and 3, respectively. The antimicrobial activity of No. 3 thyme essential oil against 4 tested molds was stronger than that of No. 1 and 2. At room temperature, the antimicrobial packaging cartons with No. 3 thyme essential oil at a volumn concentration of 4% significantly decreased the rot rate and the total number of colonies of strawberries, and also showed a positive effect on maintaining the sensory quality, which effectively extended the shelf life of the strawberries by approximately 1-2 d.
  • SUN Yanqin, ZHANG Fangfang, SHEN Juan, WU Xinyi, LI Chunbao, CHEN Ying, WANG Wei
    Food and Fermentation Industries. 2020, 46(11): 264-268. https://doi.org/10.13995/j.cnki.11-1802/ts.023361
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    Based on the optimized pretreatment method and instrument detection conditions, an inhibitory ion chromatography method was established to simultaneously detect the contents of nitrate and nitrite in livestock, poultry and their products. The samples were homogenized, extracted by ultrasound, and then separated by static separation. After removing protein, fat, and other substances by dichloromethane extraction, the supernatant was filtered and purified. And then the purified samples were analyzed by ion chromatography (IC) using KOH solution as eluent and detected by a suppressed conductivity detector. The results showed that the separation degree of nitrate and nitrite was good, the quantitation limit were 0.3 and 0.2 mg/kg and the detection limit were 0.09 and 0.06 mg/kg. Nitrate and nitrite showed good linear relationship in the range of 0.2-2.0 mg/kg and 0.02-0.2 mg/kg, respectively. Three concentration levels, low, medium and high, were labeled and measured six times in parallel. The average nominal recovery rates of nitrate and nitrite in pig, cattle, sheep, chicken and duck were between 77.0%-106.1%, 77.3%-101.0%, 65%-97.5%, 80.0%-103.3% and 90.5%-99.2%, respectively. And the average relative standard deviation (RSD) was less than 8.75%. The method established in this study is simple and fast, with accurate and reliable and can be used for simultaneous detection of nitrate and nitrite in livestock, poultry and their products.
  • MA Shuang, HAO Shuxian, LI Laihao, YANG Xianqing, HUANG Hui, CEN Jianwei
    Food and Fermentation Industries. 2020, 46(11): 269-276. https://doi.org/10.13995/j.cnki.11-1802/ts.023507
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    To explore the differences in the volatile compounds in six common caviar, the volatile compounds were identified and analyzed by solid phase micro-extraction-gas chromatography-mass spectrometry. The main flavor of different kinds of caviar was evaluated by relative odor activity value (ROAV). The results showed that a total of seven categories and sixty-three volatile aroma components were identified by GC-MS. There were some differences in the species and proportion of volatile compounds of different kinds of caviar. The total volatile component area of large yellow croaker caviar was the largest and that of salmon caviar was the least. According to ROAV, aldehydes were the main contributing components of caviar flavor. After the principal component analysis, eight substances such as diisodecyl phthalate and alpha-cedrene could be used as the characteristic components to distinguish the six caviars. The results provided reference value for the study of flavor composition of different kinds of caviar and which also could be used for distinguishing caviar species.
  • LI Weiqin, JIANG Feng, CAO Weiwei, WU Ting, PAN Siyi, XU Xiaoyun
    Food and Fermentation Industries. 2020, 46(11): 277-282. https://doi.org/10.13995/j.cnki.11-1802/ts.023619
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    An electrochemical method for the determination of capsaicin was established using an unmodified screen-printed electrode. The effects of ethanol ratio, electrolyte solution variety, pH value and KCl concentration on the determination of capsaicin by cyclic voltammetry using the screen-printed electrode were studied. The feasibility of the method was tested by stability, precision, detection limit and recovery. Results showed that the optimal parameters for the determination of capsaicin by using this unmodified screen-printed electrode were as follows: 10% ethanol, pH 1 and 80 mmol/L KCl, and Britton-Robison, CH3COOH—CH3COONa and NaH2PO4—Na2HPO4 electrolyte solutions were all suitable for the determination of capsaicin. Linear calibration curve for capsaicin in the range of 0.80~107.72 μmol/L was obtained with a detection limit of 0.13 μmol/L. In addition, the method was of good stability, precision and recovery, which was consistent with the determination of capsaicin by HPLC. Therefore, this method could be used for the rapid determination of capsaicin.
  • LI Qing, LAN Mengzhe, SONG Guanglin, WANG Daxia, SHANG Guanjia
    Food and Fermentation Industries. 2020, 46(11): 283-287. https://doi.org/10.13995/j.cnki.11-1802/ts.022113
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    This study analyzed eight preservatives in baked foods using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The procedure was as follows: the grease and organic layers were removed using hexane then followed by sample extraction, centrifugation, and resolution using the mixture of protein precipitant and absolute ethanol. The sample solution was further purified using HLB solid phase extraction column and a high concentration of nitrogen was blown over the sample. Moreover, the residue was dissolved in 1 mL of 50% methanol solution and the organic membrane was passed through a 0.22 μm filter. Acetonitrile and 5 mmol/L ammonium acetate solution were detected by liquid chromatography mass spectrometer and used as mobile phase. The results showed that eight preservatives including benzoic acid, sorbic acid, dehydroacetic acid, natamycin, methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, propyl p-hydroxybenzoate, and butyl p-hydroxybenzoate showed good linearity in the range of 0.01-50 mg/kg. And the correlation coefficient, limit of detection, limit of quantitation, recovery, and relative standard deviation were 0.998 67-0.999 84, 0.01-0.1 mg/kg, 0.04-0.4 mg/kg, 82.5%-10.5%, and 4.7%-9.6%, respectively. The LC-MS could test above eight preservatives with good recovery, steady precision, and accurate detection limit. This is suitable for high-throughput detection of multiple kinds of preservatives in food.
  • WU Xunzhong, ZHI Jinglei, KAN Jianquan, WU Yun, DU Muying
    Food and Fermentation Industries. 2020, 46(11): 288-294. https://doi.org/10.13995/j.cnki.11-1802/ts.022760
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    In order to evaluate the comprehensive quality of fermented chili, the high-performance liquid chromatography (HPLC) was used to detect the contents of eight biogenic amines in the fermented chili samples from Yunnan, Guizhou, Sichuan, Chongqing and other places in China. The contents of reducing sugar, total acid, and amino nitrogen were measured and the sensory analysis was performed. The total biogenic amines were between 3.281 and 398.831 mg/kg. Tyramine was detected in all the samples, and its content in Zao pepper was significantly higher than any other samples, while in pickled peppers was generally between 3 and 8 mg/kg. The content of reducing sugar, total acid and amino acid nitrogen in the samples was in the range of 0.06%-10.21%, 0.18-1.85 g/100 g and 0.06-0.45 g/100 g, respectively. Sensory analysis showed that the acceptance of Zao pepper and pickled pepper was higher than others. The significant difference in contents of biogenic amines may be related to factors such as the type of raw materials, manufacturing process and fermentation time.
  • CAO Xi, WANG Dan, YUAN Yongjun
    Food and Fermentation Industries. 2020, 46(11): 295-301. https://doi.org/10.13995/j.cnki.11-1802/ts.023932
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    Various selectivities make lipases become one of the most widely used biocatalysts, besides common advantages of enzymes including mild reaction conditions and biodegradability. Since positional distribution of fatty acids can significantly affect the nutritive value and physicochemical properties of triacylglycerols, lipase regioselectivity has become the major concern in the field of synthesis of functional structured triacylglycerols. This paper reviewed the progress of lipase regioselectivity in the aspects of classification, analysis methods and influence factors. Moreover, applications of regioselectivity in synthesis of functional structured triacylglycerols were particularly introduced, such as cocoa butter equivalents and improvers, human milk fat substitutes and MLM type structured lipids.
  • JIN Siyuan, XIE Jing
    Food and Fermentation Industries. 2020, 46(11): 302-306. https://doi.org/10.13995/j.cnki.11-1802/ts.023613
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    Fresh-cut fruits and vegetables are full of nutrition, but due to juice outflow after fresh-cut and their own metabolism mechanism, postharvest decay frequently occurrs, resulting in economic losses and potential health hazards. In this paper, the main rot-causing bacteria species in fresh-cut fruits and vegetables, such as Pseudomonas and Erwinia, were reviewed. The effective sterilization technology, such as biological (plant natural extract and antagonistic bacteria), physical (ultraviolet bacteriostatic technology, pulse light bacteriostatic technology, and high voltage pulse electric field bacteriostatic technology etc.) and chemical sterilization as well as their sterilization mechanism were summarized. Based on overall consideration of various sterilization methods, the existing problems and the trends for future development were proposed.
  • CAI Xiaoxia, YUAN Jing, LIN Tong, Guo Xujing, XIONG Yonghua, WU Kesheng, GUO Liang
    Food and Fermentation Industries. 2020, 46(11): 307-314. https://doi.org/10.13995/j.cnki.11-1802/ts.023443
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    Due to the high toxicity and wide distribution of (ochratoxin A,OTA), it's essential to establish facile and ultrasensitive methods for the detection of OTA in complex matrices. Benefiting from the high stability of aptamer and easy magnetic handling of magnetic material, the magnetic material-based aptasensor could achieve a rapid magnetic separation and enrichment of analytes or detection probes to improve the detection sensitivity and shorten the detection duration effectively which made it extremely suitable for the detection of trace OTA in a food sample. Herein, the recent progress in magnetic material-based OTA aptasensor using different output signals was summarized, with special emphasis on the applications of magnetic materials, novel nanomaterials and biotechnology, as well as their mechanisms in amplifying detection signals and improving the detection sensitivities, aiming at providing a reference for the design of aptasensor and ultratrace detection of mycotoxin.
  • SUN Honglai, BI Yunjie, SHI Deyou, ZHU Xuetong, LI Lili, N.V.Zaimenko, N.V.Skrypchenko, LIU Dejiang
    Food and Fermentation Industries. 2020, 46(11): 315-320. https://doi.org/10.13995/j.cnki.11-1802/ts.023715
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    The aim of this paper was tried to investigate the problems of restricting the industrial development of soft jujube Actinidia arguta products. This paper systematically summarizes the advantages and disadvantages of processing technology of all kinds of processing products of soft jujube Actinidia arguta. And it also analyzed the current situation and the solutions for storage and preservation problems. As a medicinal and edible plant, which is rich in many kinds of vitamins, minerals, dietary fiber and other nutrients, the novel product development and the improvement of the storage and preservation technology have become crucial to research. It is also critical to simplify the production process and keep its nutritional value, good physiological quality and longer shelf life. The research on soft jujube Actinidia arguta has a good prospective future.