25 November 2011, Volume 37 Issue 11
    

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    Food and Fermentation Industries
  • He Ren-yan~, Cai Yu-jie~, Liao Xiang-ru~, Li Ting-ting~, Zhang Da-bing~
    Food and Fermentation Industries. 2011, 37(11): 1-5. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.026
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    In an experiment to optimize the culture conditions for the catalase(CAT) production by Serratia marcescens SYBCT02,an interesting phenomenon occurred that the addition of ctric acid to the culture medium significantly stimulated the accumulation of CAT.To explore the reason for this finding,an experiment was designed based on the previous reports.Three kinds of carbon source,i.e.glucose,ctric acid and a mixture constituted by glucose and citric acid,with the same amount of carbon element,were used in the culture medium for CAT production by Serratia marcescens SYBCT02.After determining the contents of cellular antioxidant substrates and the activities of cellular antioxidant enzymes,an occurrence of oxidative stress was found in the cells of Serratia marcescens SYBCT02 cultivated in the media with citric acid.Compared with the control,the increased amounts of hydrogen peroxide(H2O2) and hydroxyl radical(? OH ) induced the synthesis of CAT after an addition of citric acid to the culture medium. This conclusion was further confirmed by the addition of exogenous reactive oxygen(produced by menadione and paraquat) to the culture media.
  • Yang Huan-huan~, Gao Jian ~, Xu Hong~, Shao Rong~
    Food and Fermentation Industries. 2011, 37(11): 6-10. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.027
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    To increase the quality of R,R-2,3-Butanediol using inulin extract from Jerusalem artichoke tubers by Paenibacillus polymyxa ZJ-9 and decrease the costs of production,the fermentation conditions including cultural temperature, initial pH,inoculum concentration and agitation speed were optimized.The optimal conditions were confirmed as follows;cultural temperature 30℃,the optimization initial pH 6.0,inoculum concentration 8%(v/v).A two-stage agitation speed control strategy was proposed based on analyzing the microbiol physiological characteristics under different agitation speeds.During the first 24 h,agitation speed was controlled at 240 r/min to obtain high specific cell growth rate for cell growth,subsequently agitation speed was controlled at 160 r/min to maintain high specific R,R-2,3-BD formation rate for high R,R-2,3-BD accumulation.The concentration of the obtained R,R-2,3-BD was 27.83 g/L under this optimal conditions,increase 10.15%than that before optimization.
  • Dou Wen-fang~, Zhang Dan-dan~, Xu Hong-yu~, Jin Jian~, , Xu Zheng-hong~, , Shi Jin-song~, Lu Zhen-ming~
    Food and Fermentation Industries. 2011, 37(11): 11-15. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.001
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    To systematically study the relationship between foreign protein expression and relative cell viability caused by different modules,we investigated the recombinant human hIFNβ-HSA fusion protein in Pichia pastoris KM71/IH.The results showed that;(1) There were positive correlations between the expression of foreign protein and relative cell viability;(2) Cells on midanaphase of logarithmic growth were much more suitable for foreign protein expression,because of high cell viability;(3 ) Environmental factors of induction period directly influenced the relative cell viability.The methanol concentration,induction pH and temperature were optimized to promote expression of proteins.The optimal induction conditions to express hIFNβ-HAS is;21~24 h yeast cells,2%(V/V) methanol,pH 6.0,25℃.The relative cell viability could remain more than 98%under those conditions,and the yield of hIFNβ-HSA reached 45.6 mg/L,which was 62.3%higher than that of the control.
  • Tang Bin, Zhang Feng-qin, Tang Wen-jing, Ding Li-xia
    Food and Fermentation Industries. 2011, 37(11): 16-20. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.002
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    Xylose reductase and xylitol dehydrogenase from Candida shehatae amplified by PCR were ligated to yeast expression vector pYES2,to establish recombinant expression vectors pYES2-XYL1 and pYES2-XYL2,respectively. Recombinant expression vector pYES2-XYL1-XYL2 which was acquired by legating XYL1 harboring GAL1 promoter cloned from pYES2-XYL1 to pYES2-XYL2 downstream was transformed to Saccharomyces cerevisiae host strain INVSc1 by electroporation.The ethanol yield was 33.45 g/L under the conditions;initial pH5.5,temperature at 33℃,rotation speed at 50r/min after 150 r/min for 5 h.
  • Li Jing-jing, Li Li
    Food and Fermentation Industries. 2011, 37(11): 21-25. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.047
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    Proteolysis of soybean protein isolated(SPI) by A.elegans and R.oligosporus proteases,which were cultured in their optimal enzyme production conditions,under acidic conditions pH3.0~6.0 was investigated.The results showed that,A.elegans and R.oligosporus proteases were able to quickly catalytic soybean 7s and 11s protein degradation when pH was 3.0~3.5,but at the moment,water - soluble protein yield were not high,all under 35%. At pH5.0,5.5 and 6.0,A.elegans and R.oligosporus protease all had stronger catalytic ability of degradation of SPI,both the hydrolysis degree and water-soluble protein increased,but the water - soluble protein yield from A.elegans had always been about 10%higher.SDS - PAGE showed the catalytic rate of A.elegans protease to soybean protein isolated reduced,and appeared with new bands,especially basic subunits was not able to be degraded in four hours.The speed of R.oligosporus protease catalytic degradation of soybean protein isolated was still stronger at pH5.0 and 5.5,but declined obviously at pH6.0,The acidic subunits and basic subunits were not degraded in four hours.In a word,there had big differences between the two proteases on catalysis characteristics at acidic condition.
  • Sang Min~, Liu Jian-hui~, Zhang Cheng-wu~, Li Ai-fen~
    Food and Fermentation Industries. 2011, 37(11): 26-29. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.003
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    In this paper,the effects of five kinds of nitrogen source including NaNO_3,NH_4Cl,NaNO_3 and NH_4Cl,NH_4NO_3 and NH2CONH2 on the growth,fatty acid composition and EPA content of P.pyrenoidosus CCMP 2078 was studied.The results showed that the nitrogen source had significant influence on the growth of P.pyrenoidosus CCMP 2078.The highest cell density 8×10~6 cells/mL was obtained when cultured with NH2CONH2,while the cell density in the mixture of NaNO_3 and NH_4Cl was higher than that in NH_4NO_3.With very low cell density,the growth was very slow either in NaNO_3 or NH_4Cl.The fatty acid composition was different under different nitrogen source conditions.However,the majority fatty acid were C14:0,C16:0 and EPA(C20:5).The highest PUFAs content was 24.27%when used NaNO_3 as nitrogen source,while the highest EPA content obtained was 12.93%when used NH_4NO_3 as nitrogen source.
  • Tang Ya-li~, Lu Li-xin~, Zhao Wei~;
    . , Li Chun-yang~
    Food and Fermentation Industries. 2011, 37(11): 30-32. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.048
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    The effects of high - intensity pulsed electric field on inactivation and cell membrane of E.coli were investigated.The results showed that when the applied electric intensity was higher than 20 kV/cm for 200μs,the number of survivable E.coli significantly decreased.The flow cytometry analysis of E.coli cells stained with PI showed high - intensity pulsed electric field could destroy the cell membrane and increase the permeability.When the applied electric intensity was higher than 20 kV/cm for 200μs,the PI stained cells percentage by flow cytometry sharply increases.
  • Lv Meng-sha~, Mei Can-hui~, Li Bian-sheng~, Ruan Zheng~, Zeng Xian-ze~
    Food and Fermentation Industries. 2011, 37(11): 33-38. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.049
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    In order to study the effects of pre-drying process on color and texture characteristics of ready-to-eat fermented yellow croaker before and after thermal sterilizing,the desalted samples were dried to different moisture content and then sterilized.The changes of chromatic attributes,texture attributes and liquid loss rate were investigated, with un-dried samples as control.Results showed as follows;The color and texture data of different sampling points had significant difference(P < 0.05 ).During the drying process,in general,the L* value decreased,a~* and b~* values both increased;the hardness and cohesiveness increased initially and decreased subsequently;springiness, cohesiveness and resilience decreased;the shear force and toughness increased.After thermal sterilizing,in general, the L~* value decreased,a~* and b~* values both increased;the hardness,springiness,cohesiveness,chewiness and resilience all decreased,while the shear force and toughness both increased.The color changed to a less extent for a range of moisture content between 57%and 54%,as the texture did when moisture content was between 48%and 45%.Besides,the samples had low liquid loss when moisture content was less than 51%.In conclusion,the pre-drying process has relatively great influence on the color and texture characteristics of ready-to-eat fermented yellow croaker before and after thermal sterilizing.Proper drying degree is beneficial to reduce the damage of thermal sterilizing. Considering the color,texture and liquid loss,drying to 51%moisture content is suitable.
  • Zhao Jian-xin, Wang Miao, Mao Bing-yong, Zhang Hao, Chen Wei, Tian Feng-wei, Tang Jian
    Food and Fermentation Industries. 2011, 37(11): 39-45. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.050
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    Lactobacilli and yeast play important roles in the fermentation of soybean paste.Contents of total acids,pH,alcohol content and quantities of lactobacillus and yeasts were investigated during fermentation of soybean paste with inoculation of Lactobacillus plantarum and Zygosaccharomyces rouxii.Response surface methodology was applied in order to optimize the procedures.The stationary point obtained from response surface model was the saddle point and the ridge analysis was developed to further study the results.Under the optimal condition of temperature 32.4℃,2.87×10~6 CFU/g Lactobacillus plantarum inoculation,4.55×10~5 CFU/g Zygosaccharomyces rouxii inoculation and pH 5.3 at which Zygosaccharomyces rouxii was inoculated,the maximum sensory evaluation score of 91.2 was obtained and the quality of soybean paste was noticeably improved.
  • Zeng Zhe-ling~, Zhou Xing~, Zou Qiang~, Wang Hui-lin~
    Food and Fermentation Industries. 2011, 37(11): 46-50. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.051
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    Standing stability,centrifugal stability and thermal stability were tested to evaluate emulsifying property of Polyglycerol Caprate when the oil/water ratio was 1:1.The antimicrobial activity of Polyglycerol Caprate against food spoilage and foodborne pathogens were tested by the agar diffusion method and tube double dilution method.The results showed that;the type of emulsion is Oil in Water(O/W) and the emulsion has the best indexes of emulsifying property when the amount of Polyglycerol Caprate was 4%.Antibacterial experiment indicated that Polyglycerol Caprate presented significant antimicrobial activity against the indicator fungi and bacteria.The minimum inhibition concentration (MIC) of Polyglycerol Caprate against fungi and bacteria are as follow;A.niger 0.16 mg/mL,S.cerevisae 0.16 mg/ mL,S.aureus 0.32 mg/mL,E.coil 2.5mg/mL and B.subtilis 2.5 mg/mL.
  • Zhang Yan, Zhang Cui-ying, Liu Yu-lan, Wang Dong-sheng, Xiao Dong-guang
    Food and Fermentation Industries. 2011, 37(11): 51-55. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.052
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    Spore-producing and haploid matching(a/α) experiments were carried out to obtain 5 haploids with mating type a,and 7 haploids with mating type a from the parental diploid BY-14.The fermentation capability of the haploid strains were studied,and two haploids(17αand 70a) with high maltose fermentation ability and low glucose effect degree were identified to investigate the fermentation kinetics in maltose-free,glucose-free and mixed LSMLD medium respectively.Both the haploids had short lagging phases of maltose metabolism after glucose exhaustion, which provide a good base for breeding more excellent yeast strain with higher speed of maltose metabolism.
  • Zhou Hong-lu, Li Meng-qin, Feng Lei
    Food and Fermentation Industries. 2011, 37(11): 56-59. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.053
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    In this paper,the fermentation ability of 4 kinds of active dry yeast were studied.As a result,differences were shown when these yeasts were used in fermenting low-sugar dough and plain dough.The fermentation ability of Y1 was the same for low-sugar dough and plain dough.The fermentation ability of Y2,Y5 and Y4 for low-sugar dough was higher than that on plain dough in the analysis on the relative value of fermentation ability after frozen for 7 days in low-sugar dough and plain dough,respectively.Y1,Y3 and Y4 have higher relative value of fermentation ability in plain dough;Y3 and Y4 have higher relative value of fermentation ability in low-sugar dough.The sensory evaluation and texture profile analysis of frozen steamed bread showed that the Y3 has a better quality on frozen steamed bread.
  • Hu Bo-ran~, Yin Shu-zhen~, Wen Wen~, Zhu Yong~, Sun Teng-fei~, Li Fu-dong~
    Food and Fermentation Industries. 2011, 37(11): 60-65. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.054
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    The purpose of this study include the assessment of the scavenging effect(on DPPH) of dry white wine samples by ESRS and the analysis on the relation between the scavenging effect and phenolic composition.The conditions for ESRS determination indicated by the result are reaction time 10min and dilution times 30 times.The Origin 8.0 was used to integrate the samples spectroscopic of ESR.Their correlation of the scavenging activity and total polyphenol content of different varieties' dry white wines was achieved.In Sauvignon blanc,the ability of scavenge free radicals was the best since it attained to 68%and its total phenols content was 311 mg/L;the scavenging effect of dry white wines on DPPH free radicals was positively correlated with their total phenols content.
  • Yu Ying, Li Ji-ming, Jiang Wen-guang, Zhao Rong-hua
    Food and Fermentation Industries. 2011, 37(11): 66-70. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.028
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    The effects of different vintage technologies(pectases,yeasts,malolactic bacterias and fermentation temperature) on the formation of biogenic amines(histamine,2-phenylethylamine,tyramine,tryptamine,putrescine, cadaverine,spermidine and spermine) in Cabernet Gernischt wines during vinification were studied using high pressure liquid chromatography.The results showed that biogenic amines were generated mainly from alcoholic fermentation and malo-lactic fermentation during winemaking,and the former had low concentrations of biogenic amines.The concentrations of putrescine and spermine produced during alcoholic fermentation could be controlled by pectases,inoculum concentration of yeast,fermentation temperature.The biogenic amines in dry red wines were produced mostly by malolactic bacterias,and histamine and tryptamine were largely from malo-lactic fermentation.
  • Zhao Meng, Dong Hai-zhou, Liu Peng-fei, Luo Fei-fei, Yan Qian-qian
    Food and Fermentation Industries. 2011, 37(11): 71-74. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.029
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    The property of starch film improved by montmorillonite was studied.The result showed that;the water resistance of the films was improved effectively and the retrogradation of the films was delayed by adding montmorillonite .With the addition of montmorillonite increased,the tensile strength and water dissolution time gradually increased, while the elongation gradually decreased.The optical microscopy analysis of the film surface suggested that; the films surface became more flat and smooth by adding montmorillonite.The addition of montmorillonite was better less than 3%,otherwise the membrane became more brittle and the mobility of the thermoplastic starch was lower.
  • Wei Ya-jun~, Yang Li~, Ma Hui-ling~
    Food and Fermentation Industries. 2011, 37(11): 75-80. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.030
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    In order to enhance the degradation of cell wall and increase the juice yield through easy-operated pretreatment, 'Red Fuji' apple were treated with two sets of selected conditions;500 mg/L Ethylene(E1 ) and 100 mg/L Ethylene plus 60℃hot water(E2).Cell wall metabolism related enzymes,cell wall compositions and juice yield were measured.The results showed that E1 had stronger effect on ' Red Fuji' apple.The activ peak of pectin methylesterase(PME) and cellulase(CS) of ' Red Fuji' apple appeared 6d and 3d earlier than the control,respectively. The activity of polygalacturonase(PG) increased obviously than control till the 15th day.The activity of xylanase (Xyl) was essentially the same.After 12d later,the contents of pectin polysaccharides,hemicelluloses polysaccharides and cell wall material residue polysaccharides decreased to 80.1%,70.4%and 75.7%of the control and juice yield from ' Red Fuji' apple was 2.3~4.0 percentage higher than the control after 9~18 days.E2 inhibited PME activity,enhanced PG,Xyl,CS activity and increased degradation in most cell walls than the control.E2 gained 3.2%~7.1%higher of juice yield than the control during 0~9 days.
  • Ge Qing-feng~, Cui Bao-wei~, Yu Hai ~, Wu Man-gang~, Wang Jie~, Wang Zhi-jun~
    Food and Fermentation Industries. 2011, 37(11): 81-85,90. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.031
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    Three type of antioxidant with different concentrations were individually applied in the study of fat oxidation and color property of nature casings.The result showed;0.005%BHT,0.02%VE and 0.005%sodium erythorbate had a good effect to inhibit fat oxidation and to protect color fading than other testing groups.Mixture design methodology was used to optimize antioxidant composites formula,the proportion of every antioxidant were limited in the range of 0.005%to 0.01%.The regression model of relationship between proportion and color rate of K_(δE) was also established.The result were that treated with the combination of 0.005%BHT,0.01%VE and 0.005%sodium erythorbate,K_(δE) was the smallest and casing's color was the best.
  • Sun Man-man, Gu Zheng-biao, Hong Yan, Cheng Li
    Food and Fermentation Industries. 2011, 37(11): 86-90. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.032
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    This article researched the basic properties of three types of modified starch and their application in stirred yogurt.The three types of modified starch are acetylated waxy maize distarch phosphate(AWMDP),acetylated tapioco distarch phosphate(ATDP),and waxy potato distarch phosphate(WPDP).These three kinds of starch were remained in inflated granule state after heating at 95℃for 10 min,but the expansion of the average particle size and water absorption capacity were different.These may be associated with the source and the degree of cross-linking of starch.Adding 0.5%(w/w) modified starch in stirred yogurt increased the apparent viscosity,water holding capacity, and G'of the yogurt;that also reduced the shear thinning behavior of yogurt.The WPDP was better to improve physical properties of stirred yogurt than the other two kinds modified starch.
  • Pi Yu-zhen, Liu yan-ni, Yin Yi
    Food and Fermentation Industries. 2011, 37(11): 91-94. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.033
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    Corn germ protein is rich in albumin and globulin,and its amino acid composition is very close with egg white.By mixing a certain proportion of fresh milk with Corn germ protein,fresh cheese can be produced with the aroma of fresh vegetable oil.By analyzing the content of NSI cheese ripening process,the corn germ protein fresh cheese maturity is determined to be 12 days.HS-SPME combined with GC-MS method for the cheese ripening process of the analysis of volatile components is also identified.After HS-SPME/GC-MS analysis,matured corn germ protein cheese has 14.26%L-alanine ethyl ester,7.77%the Seventeenth acid and 7.60%2,3-butanedione,and ordinary fresh cream cheese has more flavor and better aroma of vegetable oil.Fresh cheese corn germ protein has only one bitterness content,which is only 3.63%,less than that of the ordinary fresh cheese.Add corn germ protein can therefore improve the flavor of fresh cheese and make fresh cheese to have more milky flavor and vegetable oil aroma.
  • Sun Yue-e~, Wang Wei-dong~, Wang Tao
    Food and Fermentation Industries. 2011, 37(11): 95-98. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.034
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    Sulfated inulin was synthesized by sulfur trioxide-pyridine method.The reaction temperature,time, reagent proportion,and the solvent volume were optimized with the degree of sulfate group(DS) as index.The better modified condition was determined,in which reaction temperature was 95℃,reaction time was 4h,reagent proportion of inulin to sulfur trioxide - pyridine was 1:4 and volume of pyridine was 25 mL.The DS was 2.81.The results of IR spectrum and UV-spectrum confirmed the structure of the sulfated inulin.
  • Sun Peng, Cheng Jian-jun, Liu Zhao
    Food and Fermentation Industries. 2011, 37(11): 99-104. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.035
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    In order to determine reaction conditions for degree of graft(DG) of soy protein isolate - maltodextrin (SPI-MD) polymerization,optimized technology for SPI-MD degree of graft was studied using the central composite rotatable design with four variables including reaction temperature,reaction time,SPI concentration and MD/SPI ratio. Response surface methodology was used to optimize graft reaction factors in order to get the different degrees of graft of the SPI-MD grafting copolymer.The results indicated that the effect order of four factors on the yield of degree of graft was as follows:reaction temperature,SPI concentration,MD/SPI ratio and reaction time.The performance conditions for 10%,20%,30%,40%degrees of graft was also performed.
  • Zhou Ming-qian, Liu Yun-he, Bao Liu-li, Lin Hai-xia
    Food and Fermentation Industries. 2011, 37(11): 105-107. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.021
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    The decoloration technics of blackberry seed oil was studied.It was found that the best condition is as follows;the use of floridin was at 9%,decoloration temperature was at 70℃and decoloration time was 15 minute. Under these conditions,the color of blackberry seed oil decreased to RO.8,Y10.0.GC/MS analysis of the fatty acid in blackberry seed oil shows that the major fatty acids were palmic acid,stearic acid,oleic acid,linoleic acid and gamma-linolenic acid,within which unsaturated fatty acid contents about 85%.The optimized decoloration technics had little effect on the component and content of fatty acid from blackberry seed oil.
  • Cai Xiu-yun~, Zhu Xiao-rong~, Bao Ru-sheng~, Zhou Li~, Wang Pu~
    Food and Fermentation Industries. 2011, 37(11): 108-112. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.022
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    Spores of Natamycin producing strain S.natalansis PM2 -5 were treated with nitrosoguanidine,and then were selected with cerulomycin which could inhibit Polyketide synthase of the macro - lactone and the growth of Natamycin that produces strain and its product synthesis.Anti-cerulomycin Natamycin high - producing strain PM4 - 18 was obtained.Its productivity was improved 1.67 times and could be inherited stably.
  • Yang Li-li, Han Ling, Zhang Li
    Food and Fermentation Industries. 2011, 37(11): 113-116. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.036
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    In order to apply excellent lactic acid bacteria strains from pastoral area from Gansu province to production of direct vat set starter(DVS),Lactococcus lactis subspecies(HM 598683),Lactobacillus acidophilus(HM 598684) and Lactobacillus casei(HM 598685) from Gansu province were implemented in mixed fermentation by 1: 0:0,0:1:0,0:0:1,1:1:0,1:2:0,2:1:0,1:0:1,1:0:2,2:0:1,1:1:1,1:2:1,2:1:1,1:1:2,the formula of mixed fermentation was determined through sensory attributes and biochemical characteristics.And the enrichment medium of mixed fermentation was also optimized.The results showed that curd time was minimum with 8.5 h,the colony forming unit(CFU) was maximum with 7.90×10~8 CFU/mL and flavour and shape in sensory attributes was best when the ratio of strains was 1:1:1.According to methylene blue test,the activity of lactic acid bacteria was highest when trypsin was selected for hydrolyzing milk protein and the CFU was up to 2.93×10~8 CFU/mL.The optimum multiplication factors were determined by using L_9(3~4) orthogonal design with the CFU up to 2.11×10~9CFU/ mL,which were yeast powder 1%,fructose 0.5%,Tween 0.2%,sodium acetate 0.5%.The rank of effect of multiplication factors on CFU was yeast powder > sodium acetate > fructose > Tween.
  • Du Lei
    Food and Fermentation Industries. 2011, 37(11): 117-120. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.055
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    In order to provide a cheap and effective heavy metal ion adsorbent,carrot slag has been used to adsorb copper ions in aqueous solution.Results show that copper ion adsorption rate increases when using a smaller size of carrot slag.The adsorption rate for carrot slag can reach 76.35%at-100 mesh.Different quality carrot slag on different initial concentration copper ions adsorption experiments show that:under the same copper ions initial concentration, addition of more carrot slag results in a higher copper ion adsorption rate.At a constant carrot slag amount different concentrations of copper ions adsorption rate all appear a minimum value.When copper ions initial concentration is 10.0mg/L and the amount of carrot slag is 0.5g,3 factors 6 level orthogonal tests shows that;the optimum adsorption conditions for the wastewater pH is 2,the temperature is 40℃and soaking time is 3 hour.20℃and 40℃copper ion adsorption isotherm results show that:carrot slag on copper ions adsorption with single molecule layer absorption dominant,and adsorption isotherm is fit to Langmuir adsorption isotherm.The copper ions Dynamic adsorption curve results show that;second kinetic model can describe the adsorption kinetics of carrot slag.
  • Chen Yi-yong~, Dou Xiang-long~, Huang You-ru~, Zhu Dong-xing~, Yu Da~
    Food and Fermentation Industries. 2011, 37(11): 121-124. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.037
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    Crude green tea chang shu shajiabang was used as raw material to prepare tea polysaccharides.It then were purified by deproteinization and decolorization.The result showed that polyamide adsorption was the best purification (deproteinization and decolorization ) methods.The optimum decolorization technology was:The content of polyamide was 6 times of crude polysaccharides,and the polyamide column was eluted with 1.5 column volumes of deionized water at flow rate of 1.5mL/min.Under this technology,the protein removing rate was 95.8%.
  • Su Ya-li, Yang Ling
    Food and Fermentation Industries. 2011, 37(11): 125-129. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.023
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    The condition of purification of anthocyanins from Berberis kaschgarica by XAD-7HP macroporous resins was studied.The tests of screened resins and single-factor were used to determin the optimal technological parameters: 4 hours of adsorption time,60%ethanol as eluant,desorption time 100 min,pH 2.0,temperature 30℃.The concentration of anthocyanins is 750 mg /L and flow rate is 1.0 mL/min.Under the above optimum conditions,content of anthocyanins and colour value increased 3.15 and 2.21 times respectively in dark purple powder final product.
  • Wang Bi-ni~, Fan Ming-tao~, Cao Wei~, Gao Hui~, Cheng Ni~, Deng Jian-jun~
    Food and Fermentation Industries. 2011, 37(11): 130-133. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.038
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    The effects of different cooking methods(high-pressure cooking,steam and boiling under normal pressure) on the total phenolic contents and antioxidant activity of the extracts from jujube were investigated.The total phenolic contents were determined using the Folin-Ciocalteu method,and the antioxidant activity were evaluated by DPPH and FRAP assays.The results showed that the total phenolic content in jujube extracts treated by high-pressure cooking and steam is higher than that by pressure treatments,especially in the pulp of jujube.But the total phenolic acids contents in the peel and pulp of jujube treated by pressure cooking are higher.The peel of jujube treated by pressure steam exhibits the highest DPPH ? scavenging activity and total antioxidant activity.The DPPH ? scavenging activity of jujube pulp from high - pressure steam is stronger,while the total antioxidant activity of pulp from pressure cooking is higher.The total phenolics especially the phenolic acids play an important role in the antioxidant activity.If jujube is treated by high-pressure treatments,steam treatment can well preserve the phenolic compounds in jujube and their antioxidant activity.In normal pressure treatments,boiling is better.
  • Bao Yi-hong, Wang Wen-qiong, Chen Ying
    Food and Fermentation Industries. 2011, 37(11): 134-140. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.039
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    Characteristics and antioxidant property of the conjugates of whey protein and six types of saccharides under various conditions of pH were studied by means of the moist-heating glycosylated reaction.It was shown that the absorbance at 420nm and 294nm of the six glycosylated proteins were significantly different after glycosylated reaction under the pH3~9(P < 0.05 ).Meanwhile,In addition to conjugate of WPI-Scruose,the free amino group contents of other six glycosylated conjugates decreased significantly(P < 0.05 ).And the reducing power,DPPH radical scavenging activity,hydroxyl radicals scavenging and inhibition of lipid peroxidation were greatly improved with the increasing of pH,except for WPI-Scruose conjugates.The WPI-Xylose conjugate had the best antioxidant property among all the conjugates,and had stronger reducing power than Vc under the condition of pH 7~9.
  • Liu Yi-juan, Wang Fang-bing, Tang Ling, Yan Ting, Lin Shao-ling, Tang Shu-ze
    Food and Fermentation Industries. 2011, 37(11): 141-145. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.040
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    The thiobarbituric acid value(TBA),peroxide value(POV),acid value(AV),and total volatile nitrogen(TV-N) of chilled pork were determined with the aim to investigate antioxidant activity of grape seed extract (GSE).Results showed that GSE effectively inhibited POV,TBA,and TV-N of the chilled pork.Treatment with 0. 05%GSE reduced POV by 62%,TBA by 68%,and TV-N by 31%compared with the blank control after 8 days, indicating the perfect antioxidant effect.GSE didn't show significant effects on inhibition of AV.The capability of inhibitory action by treating with 0.05%GSE was equivalent to that of catechin.It was greater than that of vitamin C and propolis.At the same added level,the free radical elimination ability of GSE was greater than that of vitamin C and propolis.
  • Li Zhi-ying, Guo Cheng-ye
    Food and Fermentation Industries. 2011, 37(11): 146-149. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.041
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    Microwave method was used to extract Radix astragali extracts form p-cyclodextrin derivative.The capability of removing effect on nitrite with Radix astragali was determined.The optimum experiment conditions were studied.The capability of removing nitrite with Radix astragali flavone was determined by spectrophotometry under simulated human gastric juice.The optimum conditions was as follows:volume of 40%ethanol,microwave 260w,microwave extraction time 2 min.The capability of removing Radix astragali on nitrite was improved by HP-β-CD derivative and the clearance effect was increased from 43.9%to 60.6%.
  • Gao Hui, Cheng Ni, Deng Jian-jun, Wang Bi-ni, Cao Wei
    Food and Fermentation Industries. 2011, 37(11): 150-153. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.004
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    A comparison was made on the extraction of phenolic compounds from dates pulp(Diospyros lotus L.) with different concentrations of ethanol.The result showed that ethanol with a concentration of 80%was a suitable solvent to extract phenolic compounds.Antioxidant activities of the extract with 80%ethanol concentration were measured by DPPH,hydroxyl radical scavenging activities,reducing power and chelating activity assays.The results showed that DPPH and OH radical scavenging activities and reducing power of the extracts was concentration-dependent. Chelating activity of the extract in its Na_2EDTA equivalent was 5.64mg.
  • Guo Yuan-xin~, Yang Run-qiang~, Gu Zhen-xin~, Han Yong-bin~
    Food and Fermentation Industries. 2011, 37(11): 154-158. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.024
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    Soybean has high nutritional value and health care functions because of its many active components. Development of enrichment GABA soybean food has a wide foreground.The latest research on soybean GABA accumulation methods and pathways as well as their applications in biotechnology were reviewed in this study.
  • Zhang Zhong-yuan~, Liu Jing~, Guan Xiao~, Yang Yong-jian~, Yang Cai-xia~
    Food and Fermentation Industries. 2011, 37(11): 159-165. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.012
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    In recent 30 years,near-infrared spectroscopy analysis,which is a kind of high-efficient and low-cost technology widely used for control of food quality,has been improved rapidly.Combining with novel chemometrics methods,it could be used for the analysis of food component,food classification and adulteration detection and so on. In this paper,the application of near infrared spectroscopy combined with chemometrics methods in food analysis in recent years are reviewed.
  • Zhang Qing-an~, Fan Xue-hui~
    Food and Fermentation Industries. 2011, 37(11): 169-172. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.005
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    Progress of the polyphenols antioxidant active evaluation and its measurement methods were reviewed in this paper.The future development for the evaluation methods was also predicted and suggested.
  • Zhu Lin, Ling Jian-gang
    Food and Fermentation Industries. 2011, 37(11): 173-176. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.043
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    In this paper,the main development of preservation technology of blueberry in the world were reviewed, including cold storage,modified atmosphere packaging,edible coating,1-MCP and ultraviolet radiation,etc. The details of modified atmosphere packaging technology which is the most popular and easy-to-industrialization was introduced.Further more,the trend of the development in fresh blueberry storage technologies in our country were also prospected.
  • Yan Li~, Sun Xiu-lan~, Zhang Yin-zhi~, Zhao Jian-xin~, Wang Miao~
    Food and Fermentation Industries. 2011, 37(11): 177-182. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.013
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    This research developed a TaqMan real-time PCR method to detect aflatoxigenic Aspergilli in fermented bean sauce.We designed specific primers and TaqMan probe targeted nor-l gene,a structure gene related to aflatoxin synthetic.The standard curve was established using nor-1 gene plasmid as template and applied to monitoring nor-1 gene copies in the fermentation process of bean sauce.Then the copy numbers of the nor-l gene were compared to colony -forming unit data obtained from the same set of samples by conventional count(CC) method.A correlation between RT-PCR results and CC data was observed(Pearson,r = 0.808;P = 0.098 ).Sensitivity and specificity detection results showed that the limited detection was 10 CFU/mL,and the system was specific for nor-l containing Aspergilli. The entire detection time was only six hours and had great advantages compared with the traditional CC method. This was the first time a quick,sensitive and accurate RT-PCR detection was established to detect the nor-1 gene in fermented bean sauce.This method can monitor poisoned aflatoxigenic Aspergilli before aflatoxins producing,give better control of aflatoxins and improve food security.
  • Fan Wen-lai~, Xu Yan~, Li Ji-ming~, Yin Jian-bang~, Yu Ying~
    Food and Fermentation Industries. 2011, 37(11): 183-188. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.014
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    The potentially important aroma-active compounds of Cabernet Gernischt wine were studied in this work by gas chromatography-olfactometry(GC-O) coupled with gas chromatography-mass spectrometry(GC-MS). The aroma compounds were extracted by liquid-liquid extraction.The aromas were distilled using solvent-assisted flavour evaporation(SAFE) and separated into two fractions,acidic/water soluble fraction and neutral/basic fraction.A total of 74 aroma-active compounds were identified in the Cabernet Gernischt wines,including esters,aldehydes,ketones, alcohols,acids,terpenes,phenols,methoxypyrazines,norisoprenoids,and sulfides.Of which,3-methylbutanol andβ-damascenone could be very important aroma compounds.
  • Jin Zhen-tao, Ma Yong-qing, Liu Yan, Ma Tao, Yi Wei-xue, Cai Mu-yi
    Food and Fermentation Industries. 2011, 37(11): 189-193. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.006
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    An effective method for determining glutamine in wheat oligo - peptide products with pre - column derivatization by BTI and AQC subsequently was developed.Great linear relationship existed(r =0.999 9) at the glutamine concentration range of 2~100μmol/L and the detection limits was 2μmol/L,relative standard deviation (RSD) of peak area for all standards concentration in 3 duplicates was below 1%.Recovery of standard dipeptide AQ (Ala-Gin ) was(95.94±0.22 )%.Glutamine content in wheat oligo - peptide powder was 23.54±0.49%under the optimum condition.The glutamine content in wheat oligo-peptide powder was much greater than other food-derived oligo-peptide products.Besides of the high glutamine content,the advantage of high water solubility,strong stability, strong digestivity and absorptivity,high protein content and balanced amino acid components of glutamine in wheat oligo - peptide powder will provide its widely usage in the futrue.
  • Wang Li-li, Zhao Yu, Tang Hui-lin, He Yan-ling, Lin Song
    Food and Fermentation Industries. 2011, 37(11): 194-197. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.007
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    This article presents a new Polymerase Chain Reaction-Nucleic Acid Lateral Flow(PCR-NALF) method for the rapid detection of Listeria monocytogenes(LM) in food.Following isolation of DNA from the samples, PCR with two specific primers,one labeled with biotin and the other with digoxigenin,respectively,was performed. After applying the amplified product into a lateral flow strip that is coated with avidin and anti-digoxigenin antibodies, the test result can be interpreted according to the visible lines on the strip.The specifics were studied by testing a range of Listeria strains and other food relevant microorganisms.PCR products from other nonpathogenic Listeria,other microorganisms and the primer control(PCR without template DNA) were all negative,so this method can exactly differentiate LM from other common bacteria.And there is no observed difference(x~2 =0,P >0.05) when compared with classical microbiological detection method(GB/T 4789.30-2008).As a rapid,convenient,sensitive and specific assay method,the PCR-NALF can provide an effective means for LM detection in food samples.
  • Ding Bao-mei, Fu Yan-li, Zhang Xiao-li, Rong Shao-feng
    Food and Fermentation Industries. 2011, 37(11): 198-202. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.044
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    2-phenylethanol(2-PE) was fermented with an in situ product adsorption technique(ISPR) by Sac-charomyces cerevisiae AS 2.1182.After being isolated and purified,the product was detected by gas chromatography-mass spectrometry(GC-MS),and the purity of 2-PE achieved 99.49%.The odor detection threshold of 2-PE synthesized by different ways was tested.Results indicated that recognition threshold(RT) of 2-PE produced by fermentation was moderate and differed 0.33 ag/L from that of 2-PE extracted from rose essential oil.Additionally,odoriferous quality of 2-PE acquired from different sources was identified by electronic nose.The odoriferous quality of 2-PE derived from fermentation was close enough to that extracted from rose with a similarity of 95.88%.
  • Xiang Ying~, Chen Jian~, Jin Xin~
    Food and Fermentation Industries. 2011, 37(11): 203-207,212. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.008
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    The moisture,ash,protein and amino acid,crude fat,crude fiber,total sugar,polysaccharides,and the molecular weight of the needle mushroom at the first,the second,and the third tides have been studied,which provide meaningful data for production and consumption.
  • Su Hong-xia~, Wang Yan~, Xue Jie~ . , Liu Jian~, Jiang Wei~, Cai Xu-dong~
    Food and Fermentation Industries. 2011, 37(11): 208-212. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.045
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    The aroma composition of different roses with different treatments was studied by SPME-GC-MS(solid phase microextraction-gas chromatography-Mass spectrometry chromatography).We were able to confirm that the aroma compositions quality evaluation of Edible rose are methyl eugenol,citronellol,2-phenyl ethyl alcohol and geraniol. At the same time,the varieties of rose influence the quality of rose aroma,and in this regard chongban is better than fenghua.As for the kind rose,the later the harvest time,the better the quality of rose aroma.In the process of raw material treatment,high temperature has a great deal of impact on the quality of rose aroma.Sulfur treatment helps preserve the rose aroma to certain degree.
  • Wang Feng
    Food and Fermentation Industries. 2011, 37(11): 213-215. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.015
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    To establish an UPLC method for the determination of caffeine in tea drinks.Methods;The UPLC system consisted of CAPCELL PAK MGⅡ-C_(18) column,acetonitrile - water(10:90) as a mobile phase,with a detection wavelength at 273 nm.The flow rate was 0.5mL/min and column temperature was 30℃.Results:The linear range was between 0 and 120μg/mL,R > 0.999 47.The average recovery was between 89%and 100%.The RSD was less than 10%.Conclusion;The method is simple,accurate,and reliable and can be used in the detection of tea drink.
  • Liu Yu-qin~, Zheng Zhen-jia~, Du Jin-hua~ , Wang Dai-jie~, Wang Xiao~, Liu Jian-hua~
    Food and Fermentation Industries. 2011, 37(11): 216-219. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.025
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    An efficient method was developed for the separation and purification of monacolin K acid from the crude extract of red yeast rice by pH-zone-refining counter-current chromatography(pH-ZRCCC).Monacolin K was first extracted from red yeast rice by 95%ethanol,then it was transformed from lactone to acid form by alkalization method and separated by pH-ZRCCC with the two-phase solvent system composed of petroleum ether/ethyl acetate/ methanol/water(5:5:4:6,V/V),10 mmol/L HCl in organic phase used as the stationary phase and 10 mmol/L NH_3 ? H2O in aqueous phase used as the stationary phase.The purity of monacolin K acid form from separation fraction was analyzed by HPLC and identified by UV,ESI-MS and ~1H-NMR.From 2.0 g of crude extract,147 mg of monacolin K acid form was obtained at 93.4%purity in one-step separation.
  • Zhang Min, Du Wei-na, Bi Hua, Liu Jian
    Food and Fermentation Industries. 2011, 37(11): 220-223. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.046
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    The purpose of this study is to set up purification procedure of Cangshan garlic oligosaccharide(GOS) and determination its molecular weight.The purification effects of polyamide chromatography,DEAE-cellulose (OH~- ) chromatography,ethanol precipitation method and Sephadex G-25 chromatography on GOS was explored,respectively. The molecular weight of GOS was determined by HPLC method.The results showed that polyamide column chromatography could remove 66.8%of the protein and the GOS recovery ratio was 98.9%.DEAE-cellulose(OH~- ) chromatography separated GOS into 3 groups.Ethanol precipitation method separated GOS into 8 groups.After purified by Sephadex G-25,the purification of GOS reached to 99.1%.The Mw/Mn was 1.31 and Mn was 1770.
  • Jiao Yan-li, Du Bing, Guo Xiao-ming, Ni Wan-na, Li Ming-yu, Yang Gong-ming
    Food and Fermentation Industries. 2011, 37(11): 224-228. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.009
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    In order to timely extract protein compounds from Sheep Placenta,investigation of ultrasonic -assisted extraction was carried.The entire experiment was carefully designed and optimized with response surface method (RSM).A second quadratic equation for protein extraction was finally obtained.Factors,such as extraction temperature, solid-liquid ratio,extraction time and their corresponding relationships were investigated.Results showed that the effectiveness order of four factors is as follows:solid-liquid ratio > extraction time > extraction temperature.The optimized extraction conditions were solid-liquid ratio > extraction time > extraction temperature.Under the optimal conditions,the extraction rate of protein from Sheep Placenta reached to 53.17%.
  • Tang Xu-chong~, Ye Jing~, Xu Wei~, Chen xian-wen~
    Food and Fermentation Industries. 2011, 37(11): 229-234. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.017
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    Objective:To maximize flavonid extraction yield,optimization of the extraction of total flavonoid from clerodendranthus spicatus under ultrasound assistance was achieved by response methodology.Methods:On the basis of single -factor experiments,Box-Behnken design(BBD) involving three variables at three levels was utilized to set up a quadratic polynomial regression equation.Extraction temperature,ethanol concentration and liquid/material ration, and pair-wise interactive effects of above three variables on their function were investigated by response surface analysis.Results;The optimum condition were;30min ultrasound treatment at 100%power,ethanol concentration 54%,liquid and solid ratio was 20:1,extracting temperature 70℃.Conclusions:Under these optimum conditions, the yield of total flavonoid was 7.6%.
  • Li Fen-ling, Cai Han-quan, Zhu Zi-wen
    Food and Fermentation Industries. 2011, 37(11): 235-238. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.018
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    Microwave was applied to extract polysaccharides from Phoenix tea.Single-factor test and L_9(3~4) orthogonal experimental was used in study the influence factors of the solid-liquid ratio,microwave extraction time,microwave power,times of extraction and temperature on the content of polysaccharide.Experimental results showed that:the best extraction of Phoenix tea's polysaccharides conditions were 1:40 solid-liquid ratio,extraction time 120s,microwave power of 80%(640W),2 times,and 75℃.Microwave power is the most important factor,followed by the solid-liquid ratio and times of extraction,extraction time and temperature.
  • Yang Ji~, Lu Zhi-ming~, Lu Guo-quan~
    Food and Fermentation Industries. 2011, 37(11): 239-242. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.019
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    Through single factor and multi-factor experiments,and response surface experimental design method, the best extraction process of polysaccharides by water extraction of the Citrus bergamot is studied.The result shows that:for polysaccharides,the optimal extraction conditions is 247 minutes of extracting time,91℃of extracting temperature, and the ratio of sample to extracting reagent with 1:64.Polysaccharides yield in Citrus bergamot was 4.542 and 1.776 g/100 g,respectively.Reliable experiment verified the reliability of the above optimized condition.
  • Dong Shu-guo, Lu Zhao, Cao Hong-mei
    Food and Fermentation Industries. 2011, 37(11): 243-245. https://doi.org/10.13995/j.cnki.11-1802/ts.2011.11.020
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    Ultrasound-surfactant extraction technology of total flavones from Mulbery leaves was studied.Based on the results of single factor test,orthogonal test was used to optimize the extraction conditions.The optimum extraction conditions of extracting was;mass fraction of Tween 80 solution 1.5%,the solid-liquid ratio 1:30,time of extraction 45min,temperature of extraction 30℃.The ultrasound-surfactant extraction is a fast and an efficient extraction method.