DONG Hao, LUO Dong-hui, WU Yu-luan, XIAN Yan-ping, LUO Hai-ying, Guo Xin-dong, Han Wan-qing, Xu Li-zhu
In order to strengthen the monitoring of honey authenticity,51 honey samples with δ13CHvalue( δ13C value of honey sample) less than δ13CPvalue( δ13C value of protein in honey sample) were chosen from 124 specimens from food market. In the mean time,in order to have a comprehensive assessment of these honey samples, means of element analyzer-isotope ratio mass spectrometry( EA-IRMS),liquid chromatography-isotope ratio mass spectrometry( LC-IRMS) and liquid chromatography( LC) were used to detect the δ13C values,sucrose contents,C-4 plant sugar contents and reducing sugar contents. The results indicated that the δ13CHvalues in 51 honey samples were all less than-23. 5‰,reducing sugar contents of 2 honey samples were less than 60 g /100 g and sucrose content of 1 sample exceed limit of sucrose,which was 7 g /100 g. However,it is difficult to effectively assess the authenticity of these honey samples with δ13CHvalue less than δ13CPvalue by conventional detection methods. In our work,evaluation indexes,such as δ13CP-H,△δ13CF-G,△δ13Cmaxwere used for a comprehensive assessment. The results showed that 47 of 51 honey samples were considered to be adulterated honey,with the ratio of more than 92%. The samples were found to be adulterated by adding C-3 plant hydrolyzate,the adulterated material may be the compound of several carbohydrates.